CN103272477B - Remove complex microorganism active filler and the preparation and application thereof of sulfur-containing foul material - Google Patents
Remove complex microorganism active filler and the preparation and application thereof of sulfur-containing foul material Download PDFInfo
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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Abstract
The present invention discloses a kind of the complex microorganism active filler and the Synthesis and applications thereof that remove sulfur-containing foul material.Human pallid bacillus (Ochrobactrum involved in the present invention? anthropi) SL1 and water germ (Aquamicrobium? defluvii) SU1, for containing H from Ecological Environment Research Center, Chinese Academy of Sciences's water pollution control laboratory treatment
2obtain isolate in the reactor of the odorants such as S, above-mentioned bacterial strains is preserved in China General Microbiological culture presevation administration committee's common micro-organisms center, does is deposit number respectively CGMCC? No.7400 and CGMCC? No.7399.Described complex microorganism active filler specific area is large, and porosity is high, good permeability, and resistance is little; Bacterial cell appendix amount is high, not easily runs off; Appendix has human pallid bacillus SL1 and water germ SU1, not perishable distortion in the bioreactor of purifying exhaust air, can Long-Time Service and the treatment conditions adapting to complexity.
Description
Technical field
The invention belongs to field of environmental improvement, specifically a kind of complex microorganism active filler removing sulfur-containing foul material and preparation method thereof and application.
Background technology
Can produce a large amount of odorous gas in the processes such as sewage, Treatment of Sludge, garbage disposal and compost, the sulphur-containing substances such as hydrogen sulfide, mercaptan, thioether, carbon disulfide are malodoring substance common in odorous gas.Odorant spills in air, along with the diffuses flow of air is to everywhere, pollutes surrounding environment, produces harm to health.At present, the improvement of air pollution and foul smell causes people more and more to pay close attention to, and many countries have all formulated strict discharge standard.The discharge of pollutant in strict control waste gas, and require that enterprise is before discharging pollutants, take corresponding control measures.China has made strict regulation to the discharge of volatile organic contaminant and odorant in country " emission standard for odor pollutants " (GB14554-93) within 1993, to come into effect.
Compared with other physical chemistry waste gas processing method, bioremediation has the advantages such as cost is low, easy and simple to handle, technology clean, non-secondary pollution because of it, becomes the focus of research after the nineties.Biological ratio juris utilizes the biological oxidation of microorganism that the odorant of sulfur-bearing is converted into the process of sulfate or sulphur simple substance.
But, the usual apposition growth of microorganism at carrier surface, need when running bioreactor regularly to carrier spraying nutrient solution to maintain nutrient needed for microbial growth and moisture.In the process of spray, microorganism very easily comes off from carrier surface, flows out, cause loss with unnecessary nutrient solution from the bioreactor of purifying exhaust air, causes the problem that the treatment effect of sulfur-containing foul material reduces.Further, carrier adopts composting material, wood chip, bark usually, Raschig ring, haydite etc.The specific area of the carriers such as composting material, wood chip, bark is little, and packed density is large, and the pressure loss is large, and perishable, service life is short; Raschig ring, haydite smooth surface, microorganism not easily adheres to, and more easily runs off.The inoculum of the bioreactor of purifying sulfur-containing foul material mostly is the activated sludge of sewage treatment plant, and the microorganism laundering period is long, and bioreactor starts slow.
Summary of the invention
The object of this invention is to provide a kind of the complex microorganism active filler and the Synthesis and applications thereof that remove sulfur-containing foul material, this complex microorganism active filler specific area is large, and porosity is high, good permeability, and resistance is little; Bacterial cell appendix amount is high, not easily runs off; Appendix has human pallid bacillus (Ochrobactrumanthropi) SL1 and water germ (Aquamicrobiumdefluvii) SU1, has the efficiency of higher degraded hydrogen sulfide; Packed density in the bioreactor of purifying exhaust air is low, and pressure is low, not perishable distortion, can Long-Time Service and the treatment conditions adapting to complexity.
In order to achieve the above object, the present invention is by the following technical solutions:
Remove the complex microorganism active filler of sulfur-containing foul material, described filler appendix has human pallid bacillus (Ochrobactrumanthropi) SL1 and water germ (Aquamicrobiumdefluvii) SU1 bacteria suspension; Described human pallid bacillus (Ochrobactrumanthropi) SL1, deposit number is CGMCCNo.7400, and water germ (Aquamicrobiumdefluvii) SU1 deposit number is CGMCCNo.7399.
Described filler is one or more materials in light porous polyurethane foamed blocks, granular activated carbon, volcanic rock, perlite.
Two kinds of described microbial bacteria suspensions and the mass ratio of light porous material are 50-300: 10.
In described complex microorganism active filler, human pallid bacillus SL1 cell concentration is greater than 10
6cFU/g, the cell concentration of water germ SU1 is greater than 10
6cFU/g.
Described complex microorganism active filler preparation process is as follows:
(1) inclined-plane is cultivated: cultivate respectively and prepare human pallid bacillus SL1 and water germ SU1 slant strains;
Described human pallid bacillus SL1 slant medium consists of: glucose 0.5g, KH
2pO
42.0g, K
2hPO
42.0g, KNO
32.0g, NH
4cl0.5g, MgCl
26H
2o0.2g, FeSO
47H
2o0.01g, Na
2s
2o
35H
2o5.0g, agar 20g, water 1000ml, pH value 6.8-7.2,121 DEG C of sterilizing 20min;
Described water germ SU1 slant medium consists of: glucose 0.5g, dusty yeast 0.2g, KH
2pO
42.0g, KNO
32.0g, NH
4cl0.5g, MgCl
26H
2o0.5g, FeSO
47H
2o0.01g, Na
2s
2o
35H
2o5.0g, NaHCO
31.0g, agar 15g, water 1000ml, pH value 6.0-6.5,121 DEG C of sterilizing 20min.
(2) preparation of bacteria suspension: human pallid bacillus SL1 inclined-plane thalline step (1) prepared respectively and water germ SU1 inclined-plane thalline are by 5-20% inoculum concentration fermented and cultured step by step, and separation acquisition cell concentration is 0.4-0.8g/L human pallid bacillus SL1 bacteria suspension and cell concentration is 0.4-0.8g/L water germ SU1 bacteria suspension;
The condition of described fermented and cultured is: cultivation temperature 36 DEG C, incubation time 48h, ventilation 5-200L/min, dissolved oxygen > 2.0mg/L, tank interior pressure 0.03-0.10Mpa.
Consisting of of described human pallid bacillus SL1 liquid fermentation medium: glucose 0.5g, KH
2pO
42.0g, K
2hPO
42.0g, KNO
32.0g, NH
4cl0.5g, MgCl
26H
2o0.2g, FeSO
47H
2o0.01g, Na
2s
2o
35H
2o5.0g, water 1000ml, pH value 6.8-7.2,121 DEG C of sterilizing 20min;
Consisting of of described water germ SU1 liquid fermentation medium: glucose 0.5g, dusty yeast 0.2g, KH
2pO
42.0g, KNO
32.0g, NH
4cl0.5g, MgCl
26H
2o0.5g, FeSO
47H
2o0.01g, Na
2s
2o
35H
2o5.0g, NaHCO
31.0g, water 1000ml, pH value 6.0-6.5,121 DEG C of sterilizing 20min;
After having fermented, zymotic fluid is placed in refrigerated centrifuge, in 4 DEG C, 2000-8000rpm, centrifugal concentrating 5-20min, abandoning supernatant, precipitation is respectively with sterile nutrient solution dilution, and acquisition cell concentration is 0.4-0.8g/L human pallid bacillus SL1 bacteria suspension and cell concentration is respectively 0.4-0.8g/L water germ SU1 bacteria suspension;
Consisting of of described human pallid bacillus SL1 sterile nutrient solution: glucose 0.5g, KH
2pO
43.0g, K
2hPO
43.0g, NH
4h
2pO
40.5g, MgCl
26H
2o0.2g, FeCl
30.01g, water 1000ml, pH value 6.8-7.2; 121 DEG C of sterilizing 20min;
Consisting of of described water germ SU1 sterile nutrient solution: glucose 0.5g, dusty yeast 0.2g, KH
2pO
42.0g, KNO
32.0g, NH
4cl0.5g, MgCl
26H
2o0.5g, FeCl
30.01g, NaHCO
31.0g, water 1000ml, pH value 6.0-6.5,121 DEG C of sterilizing 20min;
(3) preparation of complex microorganism bacteria suspension: human pallid bacillus SL1 bacteria suspension step (2) prepared mixes by 1: 1 volume ratio with water germ SUl bacteria suspension, obtains complex microorganism bacteria suspension; In described complex microorganism bacteria suspension, thalline total concentration is 0.4-0.8g/L;
(4) preparation of complex microorganism active filler:
A complex microorganism bacteria suspension prepared by step (3) is sprayed the surface of the light porous material of packet or grain shape by (); B () rotates mix putting into impeller with human pallid bacillus SL1 and the porous material block (or particle) of water germ SU1 bacterial cell; C (), again to light porous material surface spraying complex microorganism bacteria suspension, obtain complex microorganism active filler, 4 DEG C save backup;
Preferred repetition step (a)-(c) 2-3 time, makes equal appendix in material surface and micropore have bacterial cell, obtains complex microorganism active filler;
Described light porous material is one or more in polyurethane foamed blocks, granular activated carbon, volcanic rock, perlite;
Described light porous material density is 0.7-1.0kg/m
3, porosity is 50-96%, and particle diameter is 5-50mm;
Described complex microorganism with the form appendix of bacteria suspension on filler;
In described complex microorganism active filler, human pallid bacillus SL1 cell concentration is greater than 10
6cFU/g
(activity is filled out material), the cell concentration of water germ SU1 is greater than 10
6cFU/g
(active filler).
The invention provides human pallid bacillus (Ochrobactrumanthropi) SL1 (CGMCCNo.7400) of a strain degraded sulfur-containing foul material, the microbial inoculum prepared by this bacterium is 1.0-90m at gas flow
3/ h, time of staying 1-3min, temperature 20-37 DEG C, concentration of hydrogen sulfide is 0.1-80mg/m3, methyl mercaptan 0.1-10mg/m3, and methyl sulfide 0.1-10mg/m3 can reach more than 90% to sulfur-containing foul mass degradation efficiency such as hydrogen sulfide, methyl mercaptan, methyl sulfides.Can be used for the gas of purifying sulfur-containing foul material, solve the odor pollution problem that the processes such as sewage, Treatment of Sludge, garbage disposal and compost produce.
Human pallid bacillus (Ochrobactrumanthropi) SL1 bacterial strain feature involved in the present invention is as follows:
This bacterial strain colony colour on LB solid plate is colourless, circular, smooth surface, and neat in edge is moistening.Thalline is shaft-like, and size is 0.5-0.8 × 3.0-3.5 μm, peritrichous, Gram-negative, catalase and oxidase positive.Indoles is negative.Glucose fermentation produces acid, can utilize nitrate, not be hydrolyzed aesculin and gelatin.Can extensively utilize carbohydrate, amino acid, organic acid for carbon source.Optimum growth temperature is 20-37 DEG C.
Full-automatic magnetic beads instrument for extracting nucleic acid SmartLabAssist-16 and respective environment sample DNA extraction purification kit (Taiwan round dot Nai meter technical concern Co., Ltd) is adopted to carry out Isolation and purification to object bacterial strain DNA respectively, select bacterial universal primers F16S-27 and R16S-1492 to carry out pcr amplification, primer sequence is respectively:
F16S-27(5`-AGAGTTTGATCCTGGCTCAG-3`)
R16S-1492(5`-CGGTTACCTTGTTACGACTTC-3`)
PCR reaction condition is set as: 94 DEG C of denaturation 5min; 94 DEG C of sex change 30sec subsequently, 56 DEG C of annealing 30sec, 72 DEG C extend 90sec, circulate 25 cycles; Then 72 DEG C extend 10min; Last 4 DEG C keep 10min.Then PCR primer is connected on PMD-19T carrier, is transformed into subsequently in bacillus coli DH 5 alpha competent cell.Competent cell 50 μ l after transforming is coated on the LB culture medium containing galactoside, isopropyl-β-d-thiogalactoside and ampicillin.Cultivate after 12 hours, carry out LB liquid medium cultivation from each dull and stereotyped picking 25 single bacterial plaques of white.Cultivate after 2 hours, nutrient solution is carried out sequencing analysis.First the sequence recorded adopts DNAMAN software to carry out similitude merger, sequence merger similitude being greater than 97% is an operating unit, the representative sequence chosen in each operating unit utilizes the 16SrDNA gene order in BLAST and GenBank database to carry out homology analysis, choose more than 1400bp length to compare (Clustelxl.83), adopt ortho position to connect (NeighbourJoining) method and carry out Phylogenetic Analysis (MEGA3.1).Phylogenetic tree as shown in Figure 1.
This microbial inoculum prepares by the production technology of test tube slant kind-shaking flask kind-seeding tank-production tank-product (packaging formulation is liquid bacterial agent or solid absorption microbial inoculum).
The invention provides water germ (Aquamicrobiumdefluvii) SU1 (CGMCCNo.7399) of a strain degraded sulfur-containing foul material, the microbial inoculum prepared by this bacterium is 1.0-50m at gas flow
3/ h, time of staying 1-3min, temperature 20-37 DEG C, concentration of hydrogen sulfide is 0.1-50mg/m
3, methyl mercaptan 0.1-20mg/m
3, can more than 85% be reached to the sulfur-containing foul such as hydrogen sulfide, methyl mercaptan mass degradation efficiency.Can be used for the gas of purifying sulfur-containing foul material, solve the odor pollution problem that the processes such as sewage, Treatment of Sludge, garbage disposal and compost produce.
Water germ SU1 bacterial strain feature involved in the present invention is as follows:
This bacterial strain colony colour on LB solid plate is white, opaque, circular, and the smooth of the edge is moistening.Thalline is rod-short, and size is 0.5-0.8 × 1.5-2.0 μm, and amphitrichous is movable, Gram-negative, catalase and oxidase positive.Glucose fermentation produces acid, can utilize nitrate, not be hydrolyzed lysine, arginine and gelatin.Utilize carbohydrate, organic acid for carbon source on a small quantity.Optimum growth temperature is 25-37 DEG C.
Full-automatic magnetic beads instrument for extracting nucleic acid SmartLabAssist-16 and respective environment sample DNA extraction purification kit is adopted to carry out Isolation and purification to object bacterial strain DNA respectively, select bacterial universal primers F16S-27 and R16S-1492 to carry out pcr amplification, primer sequence is respectively:
F16S-27(5`-AGAGTTTGATCCTGGCTCAG-3`)
R16S-1492(5`-CGGTTACCTTGTTACGACTTC-3`)
PCR reaction condition is set as: 94 DEG C of denaturation 5min; 94 DEG C of sex change 30sec subsequently, 56 DEG C of annealing 30sec, 72 DEG C extend 90sec, circulate 25 cycles; Then 72 DEG C extend 10min; Last 4 DEG C keep 10min.Then PCR primer is connected on PMD-19T carrier, is transformed into subsequently in bacillus coli DH 5 alpha competent cell.Competent cell 50 μ l after transforming is coated on the LB culture medium containing galactoside, isopropyl-β-d-thiogalactoside and ampicillin.Cultivate after 12 hours, carry out LB liquid medium cultivation from each dull and stereotyped picking 25 single bacterial plaques of white.Cultivate after 2 hours, nutrient solution is carried out sequencing analysis.First the sequence recorded adopts DNAMAN software to carry out similitude merger, sequence merger similitude being greater than 97% is an operating unit, the representative sequence chosen in each operating unit utilizes the 16SrDNA gene order in BLAST and GenBank database to carry out homology analysis, choose more than 1400bp length to compare (Clustelxl.83), adopt ortho position to connect (NeighbourJoining) method and carry out Phylogenetic Analysis (MEGA3.1).Phylogenetic tree as shown in Figure 2.
This microbial inoculum prepares by the production technology of test tube slant kind-shaking flask kind-seeding tank-production tank-product (packaging formulation is liquid bacterial agent or solid absorption microbial inoculum).
Another object of the present invention utilizes complex microorganism active filler to carry out odorous gas process, particularly at degraded H
2application in S gas, concrete steps are as follows:
(1) there is by appendix the complex microorganism active filler of human pallid bacillus SL1 and water germ SU1 to be placed in a container, pass into H
2s gas, with H
2s gas is sulphur source, the active filler of activation complex microorganism; Soak time is 1-14 days;
Described H
2the concentration of S is 50-200ppm;
(2) the complex microorganism active filler after activation is placed in bioreactor, pass into the gas of sulfur-containing foul material, with the sulfur-containing foul material in human pallid bacillus SL1 and water germ SU1 filler purifying exhaust air, complex microorganism active filler volume is 0.001-200m
3, the total concentration of sulfide is 50-200ppm, and gas flow rate is 0.05-10000m
3/ h, purification temperature is 10-35 DEG C; Regularly maintain the activity of complex microorganism active filler to complex microorganism active filler surface spraying nutrient solution, spray frequency is spray 1 time in 1-7 days, and each spray rate is 1.0-200L/min, and each spray time is 20-120min;
Consisting of of described nutrient solution: glucose 0.5g, dusty yeast 0.2g, KH
2pO
42.0g, K
2hPO
42.0g, KNO
32.0g, NH
4c10.5g, MgCl
26H
2o0.2g, FeSO
47H
2o0.01g, water 1000ml, pH6.8-7.2; The degradation capability of tracing detection sulfur-containing foul material.
The present invention adopts gas chromatography tracing detection sulfur-containing compound; Described gas chromatography is: adopt Agilent GC-6890N chromatographic to measure sulfide concentration, chromatographic column is DB-1701 capillary column (30m × 0.32mm × 0.25 μm), vaporizer, FPD detector, column temperature are set to 100 DEG C, 250 DEG C, 220 DEG C respectively, sample size 10 μ L, N2 flow velocity is 0.3mL/min, and total flow is 12mL/min.
Human pallid bacillus (Ochrobactrumanthropi) SLl involved in the present invention and water germ (Aquamicrobiumdefluvii) SU1, for containing H from Ecological Environment Research Center, Chinese Academy of Sciences's water pollution control laboratory treatment
2isolate is obtained in the reactor of the odorants such as S, oneself is preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center on April 02nd, 2013, deposit number is respectively CGMCCNo.7400 and CGMCCNo.7399, preservation address: No. 3, Yard 1, BeiChen xi Road, Chaoyang District, Beijing City, Institute of Microorganism, Academia Sinica, postcode 100101.
Beneficial effect:
1. complex microorganism active filler appendix of the present invention has superiority bacterium human pallid bacillus SL1 and water germ SU1, has the efficiency of higher degraded hydrogen sulfide; And the laundering period of dominant bacteria is short, degradation reaction can be started fast, be applied in the waste gas bioreactor of purifying sulfur-containing foul material, can the starting period be shortened.
Single bacterium only has higher removal effect to a kind of sulfur-containing foul material, and when being mixed with multiple sulfur-containing foul material in gas, clearance obviously declines.Two kinds of bacterium mix the mist that can effectively process containing multiple odorants such as hydrogen sulfide, mercaptan, thioethers.Further, when the concentration of sulfur-containing foul material is higher in gas, 100mg/m is greater than
3time, clearance still can keep more than 90%.
2. utilize the effect of sedimentation, attachment and the multiple mechanics such as centrifugal, by superior strain human pallid bacillus SL1 and water germ SU1 appendix on light porous material, form complex microorganism active filler, bacterial cell can not only be attached to filling surface equably, and can enter in the hole of filler inside, make the bacterial cell appendix amount of complex microorganism active filler high, not easily run off from bioreactor.
3. complex microorganism active filler uses front through overactivation, and bioreactor can be made to start fast.The bacterial cell enzyme of appendix is lived stable, can maintain the clean-up effect of sulfur-containing foul material steady in a long-term.
4., for rough surface, the porous of the carrier material of complex microorganism active filler, be suitable for bacterial cell attachment; Specific area is large, good permeability, and resistance is little, is conducive to bacterial cell and contacts with the sulfur-containing foul material in waste gas, improves mass-transfer efficiency.
5., for the carrier material light weight of complex microorganism active filler, be applied in the waste gas bioreactor of purifying sulfur-containing foul material, packed density is low, and the pressure loss is little.
6. the not perishable distortion of complex microorganism active filler, can Long-Time Service and the physical condition adapting to Various Complex.
Accompanying drawing explanation
Fig. 1 human pallid bacillus (Ochrobactrumanthropi) SL1 phylogenetic tree
Fig. 2 water germ (Aquamicrobiumdefluvii) SU1 phylogenetic tree
Detailed description of the invention
Example 1: a kind of microbial activity filler removing sulfur-containing foul material and preparation method thereof.
Remove a complex microorganism active filler for sulfur-containing foul material, described filler appendix has human pallid bacillus (Ochrobactrumanthropi) SL1 and water germ (Aquamicrobiumdefluvii) SU1; Described human pallid bacillus (Ochrobactrumanthropi) SL1, deposit number is CGMCCNo.7400, and water germ (Aquamicrobiumdefluvii) SU1 deposit number is CGMCCNo.7399;
Described filler is polyurethane foamed blocks;
Described packing density is 0.8kg/m
3, porosity is 86%, and particle diameter is 30mm;
Described complex microorganism with the form appendix of bacteria suspension on filler;
Described complex microorganism bacteria suspension and the mass ratio of polyurethane foamed blocks are 100: 10;
In described complex microorganism active filler, human pallid bacillus SL1 cell concentration is greater than 10
6cFU/g, the cell concentration of water germ SU1 is greater than 10
6cFU/g.
Described complex microorganism active filler preparation process is as follows:
(1) inclined-plane is cultivated: cultivate respectively and prepare human pallid bacillus SL1 and water germ SU1 slant strains;
Described human pallid bacillus SL1 slant medium consists of: glucose 0.5g, KH
2pO
42.0g, K
2hPO
42.0g, KNO
32.0g, NH
4cl0.5g, MgCl
26H
2o0.2g, FeSO
47H
2o0.01g, Na
2s
2o
35H
2o5.0g, agar 20g, water 1000ml, pH value 6.8-7.2,121 DEG C of sterilizing 20min;
Described water germ SU1 slant medium consists of: glucose 0.5g, dusty yeast 0.2g, KH
2pO
42.0g, KNO
32.0g, NH
4cl0.5g, MgCl
26H
2o0.5g, FeSO
47H
2o0.01g, Na
2s
2o
35H
2o5.0g, NaHCO
31.0g, agar 15g, water 1000ml, pH value 6.0-6.5,121 DEG C of sterilizing 20min.
(2) preparation of bacteria suspension: human pallid bacillus SL1 inclined-plane thalline step (1) prepared respectively and water germ SU1 inclined-plane thalline are by 10% inoculum concentration fermented and cultured step by step, and separation acquisition cell concentration is 0.6g/L human pallid bacillus SL1 bacteria suspension and cell concentration is 0.5g/L water germ SU1 bacteria suspension;
Human pallid bacillus SL1 bacteria suspension and water germ SU1 collecting cells method as follows;
Human pallid bacillus SL1 inclined-plane thalline step (1) prepared respectively and water germ SU1 inclined-plane thalline are by 10% inoculum concentration fermented and cultured step by step, cultivation temperature 36 DEG C of incubation time 48h, ventilation 100L/min, dissolved oxygen > 2.0mg/L, tank pressure 0.06Mpa, zymotic fluid is placed in refrigerated centrifuge in 4 DEG C, 5000rpm, centrifugal concentrating 10min, abandoning supernatant, precipitation is respectively with sterile nutrient solution dilution, and acquisition cell concentration is 0.5g/L human pallid bacillus SL1 bacteria suspension and cell concentration is respectively 0.5g/L water germ SU1 bacteria suspension;
Consisting of of described human pallid bacillus SL1 liquid fermentation medium: glucose 0.5g, KH
2pO
42.0g, K
2hPO
42.0g, KNO
32.0g, NH
4cl0.5g, MgCl
26H
2o0.2g, FeSO
47H
2o0.01g, Na
2s
2o
35H
2o5.0g, water 1000ml, pH value 6.8-7.2,121 DEG C of sterilizing 20min;
Consisting of of described water germ SU1 liquid fermentation medium: glucose 0.5g, dusty yeast 0.2g, KH
2pO
42.0g, KNO
32.0g, NH
4cl0.5g, MgCl
26H
2o0.5g, FeSO
47H
2o0.01g, Na
2s
2o
35H
2o5.0g, NaHCO
31.0g, water 1000ml, pH value 6.0-6.5,121 DEG C of sterilizing 20min;
Consisting of of described human pallid bacillus SL1 sterile nutrient solution: glucose 0.5g, KH
2pO
43.0g, K
2hPO
43.0g, NH
4h
2pO
40.5g, MgCl
26H
2o0.2g, FeCl
30.01g, water 1000ml, pH value 6.8-7.2; 121 DEG C of sterilizing 20min;
Consisting of of described water germ SU1 sterile nutrient solution: glucose 0.5g, dusty yeast 0.2g, KH
2pO
42.0g, KNO
32.0g, NH
4cl0.5g, MgCl
26H
2o0.5g, FeCl
30.01g, NaHCO
31.0g, water 1000ml, pH value 6.0-6.5,121 DEG C of sterilizing 20min;
(3) preparation of complex microorganism bacteria suspension: human pallid bacillus SL1 bacteria suspension step (2) prepared mixes by 1: 1 volume ratio with water germ SU1 bacteria suspension, obtains complex microorganism bacteria suspension; In described complex microorganism bacteria suspension, thalline total concentration is 0.5g/L;
(4) preparation of complex microorganism active filler: the complex microorganism bacteria suspension that step (3) is prepared by (a) sprays the surface of the light porous material of packet or grain shape by pump and spray head; Spray rate is 10L/min, and spray time is 60min; Stir, mixing speed is 100rpm simultaneously, and mixing time is 100min; Bacteria suspension is fully contacted with light porous; B porous material block (or particle) with human pallid bacillus SL1 and water germ SU1 bacterial cell is put into impeller by (), rotate mixing 50min under 10rpm rotating speed; Along with the rotation of blender, the bacterial cell being attached to light porous material surface enters into pore interior due to the effect of centrifugal force; C () to light porous material surface spraying complex microorganism bacteria suspension, stirs again simultaneously; Obtain complex microorganism active filler, 4 DEG C save backup;
Repeat step (a)-(c) 3 times, make equal appendix in material surface and micropore have bacterial cell, obtain complex microorganism active filler;
Described rotary blender is the revolution of efficient fine container, agitating type mixing apparatus, for the dryness of different proportion or the Homogeneous phase mixing of moist particle, and in mixed process, does not produce melting, the volatilization or rotten of material.Rotary blender main body is that stainless steel material makes, and volume is 0.05-10m
3, inside and outside wall polishing, without dead angle, built-in stirring vane, external rotating adds that built-in blade stirs, light porous filler and bacteria suspension radial roller in impeller, simultaneously also can horizontal and upper and lower convection current, and mixing efficiency is high.In mixer rotary course, on the one hand each component substances is fully mixed, on the other hand, due to the effect of centrifugal force, bacterial cell can enter in the hole of porous material inside, not only make the bacterial cell appendix amount of complex microorganism active filler high, and bacterial cell not easily runs off.
Example 2: a kind of microbial activity filler is at degraded H
2application in S gas.
Another object of the present invention utilizes complex microorganism active filler to carry out odorous gas process, particularly at degraded H
2application in S gas, concrete steps are as follows:
(1) there is by appendix the complex microorganism active filler of human pallid bacillus SL1 and water germ SU1 to be placed in a container, pass into H
2s gas, with H
2s gas is sulphur source, the active filler of activation complex microorganism; Soak time is 7 days;
Described H
2the concentration of S is 100ppm;
(2) the complex microorganism active filler after activation is placed in bioreactor, pass into the gas of sulfur-containing foul material, with the sulfur-containing foul material in human pallid bacillus SL1 and water germ SU1 purifying exhaust air, complex microorganism active filler volume is 100m
3, the total concentration of sulfide is 100ppm, and gas flow rate is 1000m
3/ h, purification temperature is 25 DEG C; Regularly maintain the activity of complex microorganism active filler to complex microorganism active filler surface spraying nutrient solution, spray frequency is spray 1 time for 3 days, and each spray rate is 50L/min, and each spray time is 60min;
Consisting of of described nutrient solution: glucose 0.5g, dusty yeast 0.2g, KH
2pO
42.0g, K
2hPO
42.0g, KNO
32.0g, NH
4cl0.5g, MgCl
26H
2o0.2g, FeSO
47H
2o0.01g, water 1000ml, pH6.8-7.2; The degradation capability of tracing detection sulfur-containing foul material.
Example 3: result of use experiment (filler of preparation in example 1 is carried out three experiments below respectively).
(1) dispose of sewage the gases containing stench sulphureous gases of factory's mudpan loss, gas flow is 35m
3/ h, the time of staying is 1 ~ 3mins, operates under room temperature condition.The inlet gas concentration of hydrogen sulfide is: 14.86mg/m
3, concentration of giving vent to anger is 0.03mg/m
3, lower than the emission standard for odor pollutants that country formulates, clearance reaches 99%.
(2) for the treatment of the gases containing stench sulphureous gases that sludge composting produces, the concentration of hydrogen sulfide and ethyl mercaptan is respectively 18mg/m
3and 2.53mg/m
3, gas flow is 70m
3/ h, the time of staying is 1 ~ 3mins, operates under room temperature condition, and the clearance of hydrogen sulfide and ethyl mercaptan is all greater than 90%.
(3) refuse landfill, process is containing 162.1mg/m
3hydrogen sulfide, 3.82mg/m
3methyl mercaptan, and 8.2mg/m
3the mix waste gas of methyl sulfide, gas flow is 60m
3/ h, the time of staying is 1 ~ 3mins, temperature 18-37 DEG C, and the clearance of hydrogen sulfide, methyl mercaptan, methyl sulfide reaches 95.1%, 90.6% and 91% respectively.
Example 4 is basic with example 1
Remove the complex microorganism active filler of sulfur-containing foul material, described filler appendix has human pallid bacillus (Ochrobactrumanthropi) SL1 and water germ (Aquamicrobiumdefluvii) SU1 bacteria suspension; Described human pallid bacillus (Ochrobactrumanthropi) SL1, deposit number is CGMCCNo.7400, and water germ (Aquamicrobiumdefluvii) SU1 deposit number is CGMCCNo.7399.
Described filler is granular activated carbon.
Two kinds of described microbial bacteria suspensions and the mass ratio of light porous material are 200: 10.
In described complex microorganism active filler, human pallid bacillus SL1 cell concentration is greater than 10
6cFU/g, the cell concentration of water germ SU1 is greater than 10
6cFU/g.
Described complex microorganism active filler preparation process is with example 1:
In described complex microorganism bacteria suspension, thalline total concentration is 0.4g/L;
Example 5
Remove the complex microorganism active filler of sulfur-containing foul material, described filler appendix has human pallid bacillus (Ochrobactrumanthropi) SL1 and water germ (Aquamicrobiumdefluvii) SU1 bacteria suspension; Described human pallid bacillus (Ochrobactrumanthropi) SL1, deposit number is CGMCCNo.7400, and water germ (Aquamicrobiumdefluvii) SU1 deposit number is CGMCCNo.7399.
Described filler is perlite.
Two kinds of described microbial bacteria suspensions and the mass ratio of light porous material are 50: 10.
In described complex microorganism active filler, human pallid bacillus SL1 cell concentration is greater than 10
6cFU/g, the cell concentration of water germ SU1 is greater than 10
6cFU/g.
Described complex microorganism active filler preparation is with example 1:
In described complex microorganism bacteria suspension, thalline total concentration is 0.8g/L;
Described light porous material density is 0.7kg/m3, and porosity is 50%, and particle diameter is 5-20mm.
Claims (8)
1. remove a complex microorganism active filler for sulfur-containing foul material, described filler appendix has human pallid bacillus (Ochrobactrumanthropi) SL1 and water germ (Aquamicrobiumdefluvii) SU1 bacteria suspension; Described human pallid bacillus (Ochrobactrumanthropi) SL1, deposit number is CGMCCNo.7400, and water germ (Aquamicrobiumdefluvii) SU1 deposit number is CGMCCNo.7399.
2. a kind of complex microorganism active filler removing sulfur-containing foul material according to claim 1, described filler is one or more materials in light porous polyurethane foamed blocks, granular activated carbon, volcanic rock, perlite.
3. a kind of complex microorganism active filler removing sulfur-containing foul material according to claim 1, two kinds of described microbial bacteria suspensions and the mass ratio of light porous material are 50-300: 10.
4. a kind of complex microorganism active filler removing sulfur-containing foul material according to claim 1, in described complex microorganism active filler, human pallid bacillus SL1 cell concentration is greater than 10
6cFU/g, the cell concentration of water germ SU1 is greater than 10
6cFU/g.
5. a kind of preparation method removing the complex microorganism active filler of sulfur-containing foul material according to claim 1, step is as follows:
(1) inclined-plane is cultivated: cultivate respectively and prepare human pallid bacillus (Ochrobactrumanthropi) SL1 and water germ (Aquamicrobiumdefluvii) SU1 slant strains;
(2) preparation of bacteria suspension: human pallid bacillus SL1 inclined-plane thalline step (1) prepared respectively and water germ SU1 inclined-plane thalline are by 5-20% inoculum concentration fermented and cultured step by step, and obtaining cell concentration with sterile nutrient solution dilution after separation be 0.4-0.8g/L human pallid bacillus SL1 bacteria suspension and cell concentration is 0.4-0.8g/L water germ SU1 bacteria suspension;
(3) preparation of complex microorganism bacteria suspension: human pallid bacillus SL1 bacteria suspension step (2) prepared mixes by 1:1 volume ratio with water germ SU1 bacteria suspension, obtains complex microorganism bacteria suspension; In described complex microorganism bacteria suspension, thalline total concentration is 0.4-0.8g/L;
(4) preparation of complex microorganism active filler:
A complex microorganism bacteria suspension prepared by step (3) is sprayed the surface of the light porous material of packet or grain shape by (); B () rotates mix putting into impeller with human pallid bacillus SL1 and the porous material block of water germ SU1 bacterial cell; C (), again to light porous material surface spraying complex microorganism bacteria suspension, obtain complex microorganism active filler, 4 DEG C save backup; Repeat step (a)-(c) 2-3 time, make equal appendix in material surface and micropore have bacterial cell, obtain complex microorganism active filler.
6. a kind of preparation method removing the complex microorganism active filler of sulfur-containing foul material according to claim 5, consisting of of described water germ SU1 sterile nutrient solution: glucose 0.5g, dusty yeast 0.2g, KH
2pO
42.0g, KNO
32.0g, NH
4cl0.5g, MgCl
26H
2o0.5g, FeCl
30.01g, NaHCO
31.0g, water 1000ml, pH value 6.0-6.5,121 DEG C of sterilizing 20min.
7. a kind of preparation method removing the complex microorganism active filler of sulfur-containing foul material according to claim 5, consisting of of described human pallid bacillus SL1 sterile nutrient solution: glucose 0.5g, KH
2pO
43.0g, K
2hPO
43.0g, NH
4h
2pO
40.5g, MgCl
26H
2o0.2g, FeCl
30.01g, water 1000ml, pH value 6.8-7.2; 121 DEG C of sterilizing 20min.
8. a kind of complex microorganism active filler removing sulfur-containing foul material according to claim 1-4 any one is removing the application in sulfur-containing foul material.
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| CN103432900B (en) * | 2013-09-07 | 2015-08-12 | 中蓝连海设计研究院 | The purposes of a kind of microbial bacterial agent in foul gas pollution control |
| CN103691305B (en) * | 2013-12-18 | 2016-03-30 | 中国科学院生态环境研究中心 | A kind of preparation method of the active filler containing the hot bacterium that dwells |
| CN104667475B (en) * | 2014-12-16 | 2017-09-26 | 华南农业大学 | A kind of method of utilization anthropi efficient degradation Fluoxastrobin |
| CN104531249A (en) * | 2014-12-31 | 2015-04-22 | 山东圣琪生物有限公司 | Methane desulfurizer |
| CN107011912B (en) * | 2017-04-12 | 2019-10-25 | 陕西省微生物研究所 | Pesticide carbendazim microbial inoculum for degrading and preparation method thereof |
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| CN102965294A (en) * | 2011-10-25 | 2013-03-13 | 北京农业生物技术研究中心 | Alcaligenes faecalis, method for preparation of desulfurization deodorant from the same and application |
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| CN102965294A (en) * | 2011-10-25 | 2013-03-13 | 北京农业生物技术研究中心 | Alcaligenes faecalis, method for preparation of desulfurization deodorant from the same and application |
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