CN103269762B - 亲和色谱基质 - Google Patents

亲和色谱基质 Download PDF

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Publication number
CN103269762B
CN103269762B CN201180061115.6A CN201180061115A CN103269762B CN 103269762 B CN103269762 B CN 103269762B CN 201180061115 A CN201180061115 A CN 201180061115A CN 103269762 B CN103269762 B CN 103269762B
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China
Prior art keywords
protein
ligand
domain
elution
matrix
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CN201180061115.6A
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English (en)
Chinese (zh)
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CN103269762A (zh
Inventor
T.布杰克曼
B.诺伦
G.罗德里戈
J.瓦斯
P-M.阿伯格
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Cytiva Bioprocess R&D AB
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GE Healthcare Bio Sciences Corp
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Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/12—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from bacteria
    • C07K16/1267—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from bacteria from Gram-positive bacteria
    • C07K16/1271—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from bacteria from Gram-positive bacteria from Micrococcaceae (F), e.g. Staphylococcus
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/10—Selective adsorption, e.g. chromatography characterised by constructional or operational features
    • B01D15/16—Selective adsorption, e.g. chromatography characterised by constructional or operational features relating to the conditioning of the fluid carrier
    • B01D15/166—Fluid composition conditioning, e.g. gradient
    • B01D15/168—Fluid composition conditioning, e.g. gradient pH gradient or chromatofocusing, i.e. separation according to the isoelectric point pI
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/38—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving specific interaction not covered by one or more of groups B01D15/265 and B01D15/30 - B01D15/36, e.g. affinity, ligand exchange or chiral chromatography
    • B01D15/3804—Affinity chromatography
    • B01D15/3809—Affinity chromatography of the antigen-antibody type, e.g. protein A, G or L chromatography
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/281—Sorbents specially adapted for preparative, analytical or investigative chromatography
    • B01J20/286—Phases chemically bonded to a substrate, e.g. to silica or to polymers
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/281—Sorbents specially adapted for preparative, analytical or investigative chromatography
    • B01J20/286—Phases chemically bonded to a substrate, e.g. to silica or to polymers
    • B01J20/289—Phases chemically bonded to a substrate, e.g. to silica or to polymers bonded via a spacer
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/30—Processes for preparing, regenerating, or reactivating
    • B01J20/32—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating
    • B01J20/3202—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating characterised by the carrier, support or substrate used for impregnation or coating
    • B01J20/3206—Organic carriers, supports or substrates
    • B01J20/3208—Polymeric carriers, supports or substrates
    • B01J20/3212—Polymeric carriers, supports or substrates consisting of a polymer obtained by reactions otherwise than involving only carbon to carbon unsaturated bonds
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/30—Processes for preparing, regenerating, or reactivating
    • B01J20/32—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating
    • B01J20/3214—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating characterised by the method for obtaining this coating or impregnating
    • B01J20/3217—Resulting in a chemical bond between the coating or impregnating layer and the carrier, support or substrate, e.g. a covalent bond
    • B01J20/3219—Resulting in a chemical bond between the coating or impregnating layer and the carrier, support or substrate, e.g. a covalent bond involving a particular spacer or linking group, e.g. for attaching an active group
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/30—Processes for preparing, regenerating, or reactivating
    • B01J20/32—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating
    • B01J20/3231—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating characterised by the coating or impregnating layer
    • B01J20/3242—Layers with a functional group, e.g. an affinity material, a ligand, a reactant or a complexing group
    • B01J20/3268—Macromolecular compounds
    • B01J20/3272—Polymers obtained by reactions otherwise than involving only carbon to carbon unsaturated bonds
    • B01J20/3274—Proteins, nucleic acids, polysaccharides, antibodies or antigens
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/22—Affinity chromatography or related techniques based upon selective absorption processes
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/305—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Micrococcaceae (F)
    • C07K14/31—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Micrococcaceae (F) from Staphylococcus (G)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Molecular Biology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Immunology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Peptides Or Proteins (AREA)
CN201180061115.6A 2010-12-20 2011-12-19 亲和色谱基质 Active CN103269762B (zh)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US201061424698P 2010-12-20 2010-12-20
US61/424,698 2010-12-20
US61/424698 2010-12-20
PCT/SE2011/051537 WO2012087231A1 (en) 2010-12-20 2011-12-19 Affinity chromatography matrix

Publications (2)

Publication Number Publication Date
CN103269762A CN103269762A (zh) 2013-08-28
CN103269762B true CN103269762B (zh) 2016-01-27

Family

ID=46314246

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201180061115.6A Active CN103269762B (zh) 2010-12-20 2011-12-19 亲和色谱基质

Country Status (5)

Country Link
US (1) US9187555B2 (enExample)
EP (1) EP2654914B1 (enExample)
JP (1) JP5974343B2 (enExample)
CN (1) CN103269762B (enExample)
WO (1) WO2012087231A1 (enExample)

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2013081540A1 (en) * 2011-11-30 2013-06-06 Ge Healthcare Bio-Sciences Ab Affinity chromatography matrix
EP2831096B1 (en) * 2012-03-28 2020-04-29 GE Healthcare BioProcess R&D AB Affinity chromatography matrix
BR112015005937A2 (pt) 2012-09-17 2017-07-04 Grace W R & Co meios e dispositivos cromatográficos
US9896486B2 (en) * 2013-07-10 2018-02-20 Ge Healthcare Bioprocess R&D Ab Mutated immunoglobulin-binding polypeptides
ES2887110T3 (es) 2014-01-16 2021-12-21 Grace W R & Co Medios para cromatografía de afinidad y dispositivos para cromatografía
US11389783B2 (en) 2014-05-02 2022-07-19 W.R. Grace & Co.-Conn. Functionalized support material and methods of making and using functionalized support material
US11566082B2 (en) * 2014-11-17 2023-01-31 Cytiva Bioprocess R&D Ab Mutated immunoglobulin-binding polypeptides
PL3302784T3 (pl) 2015-06-05 2022-01-17 W.R. Grace & Co.-Conn. Adsorbentowe środki klarujące do bioprzetwarzania oraz sposoby ich wytwarzania i stosowania
WO2017014259A1 (ja) * 2015-07-22 2017-01-26 株式会社カネカ 酸性ｐＨ領域での抗体結合能が低下した抗体結合性タンパク質
JP6512308B2 (ja) * 2015-12-28 2019-05-15 株式会社島津製作所 クロマトグラフ
US10730908B2 (en) 2016-05-11 2020-08-04 Ge Healthcare Bioprocess R&D Ab Separation method
US10654887B2 (en) 2016-05-11 2020-05-19 Ge Healthcare Bio-Process R&D Ab Separation matrix
US10703774B2 (en) 2016-09-30 2020-07-07 Ge Healthcare Bioprocess R&D Ab Separation method
ES2909833T3 (es) 2016-05-11 2022-05-10 Cytiva Bioprocess R & D Ab Método de limpieza y/o desinfección de una matriz de separación
US10889615B2 (en) 2016-05-11 2021-01-12 Cytiva Bioprocess R&D Ab Mutated immunoglobulin-binding polypeptides
US11708390B2 (en) 2016-05-11 2023-07-25 Cytiva Bioprocess R&D Ab Method of storing a separation matrix
JP7031934B2 (ja) 2016-05-11 2022-03-08 サイティバ・バイオプロセス・アールアンドディ・アクチボラグ 分離マトリックス
EP3455243B1 (en) 2016-05-11 2021-03-24 Cytiva BioProcess R&D AB Separation matrix
US12448411B2 (en) 2016-09-30 2025-10-21 Cytiva Bioprocess R&D Ab Separation method
CN106543274B (zh) * 2016-10-18 2020-10-27 天津大学 一种对抗体具有特异性结合作用的a蛋白结构域z的衍生物及其应用
WO2018108839A1 (en) 2016-12-12 2018-06-21 Hurrah S.À R.L. Compositions and methods for increasing the immunoglobulin binding capacities of immunoglobulin-binding polypeptides and oligopeptides
GB201703116D0 (en) * 2017-02-27 2017-04-12 Ge Healthcare Bioprocess R&D Ab A seperation matrix and a method of seperating antibodies
KR20230112616A (ko) 2020-10-13 2023-07-27 아비타이드 엘엘씨 3중-나선 번들 단백질들의 친화성 리간드 라이브러리 및 이의 용도
WO2022178396A1 (en) 2021-02-19 2022-08-25 Avitide LLC Aav2 affinity agents
GB202113626D0 (en) 2021-09-24 2021-11-10 Cytiva Bioprocess R & D Ab FC binding polypeptides
CN114939400B (zh) * 2022-06-28 2024-11-22 上海兆维科技发展有限公司 一种亲和层析填料及其制备方法以及亲和纯化工艺

Citations (3)

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Publication number Priority date Publication date Assignee Title
US6602977B1 (en) * 1999-04-19 2003-08-05 Biovitrum Ab Receptor structures
WO2005075507A1 (en) * 2004-02-09 2005-08-18 Affibody Ab Amyloid beta peptide binding polypeptide
CN1816563A (zh) * 2003-07-04 2006-08-09 阿菲博迪公司 对her2具有结合亲和力的多肽

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SE8505922D0 (sv) 1985-12-13 1985-12-13 Kabigen Ab Construction of an igg binding protein to facilitate downstream processing using protein engineering
SE0200943D0 (sv) * 2002-03-25 2002-03-25 Amersham Biosciences Ab Mutant protein
US7709209B2 (en) 2002-03-25 2010-05-04 Ge Healthcare Bio-Sciences Ab Protein ligands
DK1869071T3 (da) 2005-03-01 2011-02-28 Affibody Ab TNF-alfa-bindende polypeptid, anvendelser deraf og fremgangsmåder, der gør brug deraf
JP5229888B2 (ja) * 2008-09-30 2013-07-03 独立行政法人産業技術総合研究所 弱酸性域での易解離性を向上したプロテインａ変異型タンパク質及び抗体捕捉剤
JP2012515160A (ja) 2009-01-12 2012-07-05 ジーイー・ヘルスケア・バイオサイエンス・アクチボラグ アフィニティークロマトグラフィーマトリックス

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6602977B1 (en) * 1999-04-19 2003-08-05 Biovitrum Ab Receptor structures
CN1816563A (zh) * 2003-07-04 2006-08-09 阿菲博迪公司 对her2具有结合亲和力的多肽
WO2005075507A1 (en) * 2004-02-09 2005-08-18 Affibody Ab Amyloid beta peptide binding polypeptide

Also Published As

Publication number Publication date
CN103269762A (zh) 2013-08-28
US20130338339A1 (en) 2013-12-19
JP5974343B2 (ja) 2016-08-23
JP2014500311A (ja) 2014-01-09
EP2654914A4 (en) 2015-01-07
EP2654914B1 (en) 2018-05-30
WO2012087231A1 (en) 2012-06-28
US9187555B2 (en) 2015-11-17
EP2654914A1 (en) 2013-10-30

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Patentee after: GE HEALTHCARE BIO-SCIENCES AB

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Patentee before: GE Health Bio-science Co., Ltd.

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Patentee after: Situofan Biotechnology R & D Co., Ltd

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Patentee before: GE HEALTHCARE BIOPROCESS R&D AB