CN103215206A - Blue-green alga-rice complex operation nitrogen-fixing and carbon-fixing method - Google Patents
Blue-green alga-rice complex operation nitrogen-fixing and carbon-fixing method Download PDFInfo
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Abstract
The invention relates to a blue-green alga-rice complex operation nitrogen-fixing and carbon-fixing method. Anabaena azollae, anabaena sphaerica and anabaena flosaguas are taken as initial materials, and after expanded culture of strains in a sterile chamber and scale production in a small pool of a greenhouse, the strains are processed with the help of a screen to enable the water content to be 40%-60%; then mixing, stirring and crushing are performed at the day according to the weight ratio of 2: 6: (2-3): 4: 3, and a mixture is uniformly sprayed into a watered rice field with the density of 2-5g/m<2>; or wet algae are placed in an oven at the temperature of 35-40 DEG C for 8h or placed under a fluorescent lamp of 30-50W in the sterile chamber for drying for 3-5 days to enable the water content to be 8%-12%, crushing is performed by using a crushing machine, dry alga powder is uniformly blended according to the weight ratio of 1: 8: (1-2): 6: 2 and mixed in 5-10 times of the weight of rice husk, and the dry alga powder is uniformly sprayed into the watered rice field with the density of 0.5g/m<2>. According to the method disclosed by the invention, nitrogen-fixing blue-green algae are cultivated and stocked in the rice field as a fertilizer source, CO2 is absorbed, organic matters are generated, soil is improved, the yield and quality of paddy rice are improved, and the method has the advantages of simple process, easiness in popularization, safety, high efficiency, no pollution and the like.
Description
Technical field
The present invention relates to preparation and the application process of a kind of agricultural, be specifically related to the method for fixed nitrogen with the solid carbon of the compound operation of a kind of blue-green algae-paddy rice, belong to biological technical field with bio-bacterial manure.
Background technology
Herba azollae imbricatae anabena (Anabaena azollae, numbering: FACHB-190), fixed nitrogen anabena (Anabaena azotica, numbering: FACHB-686), wawter bloom anabena (Anabaena flos-aquae, numbering: FACHB-245) provide by Inst. of Hydrobiology, Chinese Academy of Sciences algae kind storehouse, and at " Jianying Shen, Antonio DiTommaso, Mingquan Shen, Wei Lu, and Zhengming Li; Molecular basis for differential metabolic responses tomonosulfuron in three nitrogen-fixing cyanobacteria, Weed Science, 2009,57:178-188 ", " Jianying Shen, Jing Jiang, Peizhong Zheng; Effects of Light and Monosulfuron on growth and Photosynthetic Pigments of Anabaena flos-aquae Breb, Water Resource and Protection, 2009,1:408-413 ", " Zheng Peizhong, Shen Jianying; Organic solvent-acetone is to fixed nitrogen blue algae growth Study on Effect, Shanghai Agricultural journal, 2010.4:33-38 " go up openly.
Herba azollae imbricatae anabena, fixed nitrogen anabena and wawter bloom anabena are three kinds of fixed nitrogen blue-green algaes, the fixing nomadic nitrogen in the atmosphere, and its distinctive algae somatotropin can promote that crop grows vigorously, and is remarkable to the increasing production of rice effect, generally can reach 1-4; Simultaneously as autotrophic organism, the fixing CO in the atmosphere
2, the effect that increases the soil organism, improves the soil is arranged.Because therefore the production of agrochemicals fertilizer and agricultural chemicals and use and can bring a large amount of greenhouse gas emissions develop agro-ecology fixed nitrogen technology, reduces applying quantity of chemical fertilizer, is the important channel of current development low-carbon (LC) agricultural.Along with the fast development of agricultural science and technology, bio-bacterial manure will be exploited more and more.
At present both at home and abroad to the Herba azollae imbricatae anabena, the correlative study of fixed nitrogen anabena and wawter bloom anabena also seldom, the Chinese Academy of Sciences once mentioned the induction method of high spore content in " absorption spectrum and the fluorescence spectrum characteristic of dormancy of Herba azollae imbricatae anabena and sprouting spore ", aspect the preparation of the production of fixed nitrogen blue-green algae and dry algae powder, rarely seen Shen Jian English etc. are in " inductive technology of wawter bloom anabena chlamydospore and the preparation method of dry algae powder thereof ", " the quick active method and the mass production method of fixed nitrogen anabena dry algae powder ", " preparation method of Herba azollae imbricatae anabena dry algae powder " and Shen Yinwu etc. mention in " result and prospect that the fixed nitrogen blue-green algae is cultivated and uses ", and exploration is in other respects then rarely reported.
Summary of the invention
The objective of the invention is to overcome the deficiency that prior art exists; the method of fixed nitrogen with the solid carbon of the compound operation of a kind of blue-green algae-paddy rice is provided; with three kinds of fixed nitrogen blue-green algaes is original material; after large-scale production; be processed into water content certain the algae kind; the algae kind that is mixed is in proportion also put in a suitable place to breed among the rice field, and this method has safety and characteristics easily.
The present invention is achieved by the following technical solutions:
The method of the fixed nitrogen of the compound operation of a kind of blue-green algae-paddy rice and solid carbon; with three kinds of fixed nitrogen blue-green algaes is original material; through sterilisable chamber algae kind spread cultivation and booth Xiao Chi large-scale production after, be processed into the certain algae kind of water content, these three kinds of algae kinds that are mixed in proportion and being sprinkled upon equably among the rice field of pouring water.
Described three kinds of fixed nitrogen blue-green algaes are Herba azollae imbricatae anabena, fixed nitrogen anabena and wawter bloom anabena.
The concrete steps of described method are as follows:
1) be original material with Herba azollae imbricatae anabena, fixed nitrogen anabena and wawter bloom anabena, through sterilisable chamber algae kind spread cultivation and booth Xiao Chi large-scale production after, by sieve control water, make it water content 40%～60%;
2) same day of obtaining wet algae with three kinds of fixed nitrogen blue-green algaes according to the Herba azollae imbricatae anabena: the fixed nitrogen anabena: wawter bloom anabena mass ratio is that 2:6:2～3:4:3 mixes and blends;
3) the fixed nitrogen blue-green algae after will being mixed is sprinkled upon in the rice field of pouring water equably with the density of every square metre of 2～5g.
The described same day of obtaining wet algae with three kinds of fixed nitrogen blue-green algaes according to the Herba azollae imbricatae anabena: the fixed nitrogen anabena: wawter bloom anabena mass ratio is that 2:6:2～3:4:3 mixes and blends and is meant, be blended in three kinds of wet algaes in the container that day of results blue-green algae, smashes with stirring rod.
The concrete steps of described method are as follows:
1) be original material with Herba azollae imbricatae anabena, fixed nitrogen anabena and wawter bloom anabena, through sterilisable chamber algae kind spread cultivation and booth Xiao Chi large-scale production after, by sieve control water, make it water content 40%～60%;
2) the wet algae that will obtain places 35～40 ℃ baking oven 8h, make it water content between 8%～12%, pulverize with pulverizer, with the dry algae powders of three kinds of fixed nitrogen blue-green algaes according to the Herba azollae imbricatae anabena: the fixed nitrogen anabena: wawter bloom anabena mass ratio is that 1:8:1～2:6:2 is mixed evenly;
3) dry algae powder of the fixed nitrogen blue-green algae after will being mixed is mixed in the rice husk of 5～10 times of quality, is sprinkled upon equably in the rice field of pouring water with the density of every square metre of 0.5g dry algae powder.
Described step 2) the contents are as follows:
2) the wet algae that will obtain places the fluorescent lamp of 30～50 watts of sterilisable chambers to descend dry 3～5 days, make it water content between 8%～12%, pulverize with pulverizer, with the dry algae powders of three kinds of fixed nitrogen blue-green algaes according to the Herba azollae imbricatae anabena: the fixed nitrogen anabena: wawter bloom anabena mass ratio is that 1:8:1～2:6:2 is mixed evenly.
The dry algae powder of fixed nitrogen blue-green algae after described will being mixed is mixed in the rice husk of 5～10 times of quality and is meant, rice husk as the carrier that supports described dry algae powder, is sneaked into this dry algae powder in the rice husk of 5～10 times of quality.
The processing condition that described sterilisable chamber algae kind spreads cultivation are: light intensity 3000 ± 200Lx, 30 ± 2 ℃ of temperature.
The processing condition of described booth Xiao Chi large-scale production are: Xiao Chi length * wide * high size 1～2m * 0.5～1m * 0.8～1.2m, and 30 ± 2 ℃ of temperature, natural light, each Xiao Chi add the algae kind 2～5g of water content 50%, cultured continuously 3～5 days.
The aperture of described sieve is 50～300 orders.
The method of the fixed nitrogen of the compound operation of blue-green algae-paddy rice of the present invention and solid carbon is put three main technique flow processs to cultivate the fixed nitrogen blue-green algae in a suitable place to breed by scale production-mixed algae kind-rice field, has improved the yield increase effect of paddy rice.
Beneficial effect of the present invention is: to cultivate the fixed nitrogen blue-green algae, it is put in a suitable place to breed in the rice field as the source of manure, absorbed greenhouse gases CO
2, generated organic matter, improved soil, improved rice yield and quality, have technology simple, be easy to promotion and implementation, fertilizer safe and efficient, can not be to advantages such as environment and food pollute, thereby have great theoretical value and application potential.
Tabulate down 1 is the change records of soil organic matter content after different treatment.As can be seen, the soil quality of handling through the method for the invention has had significantly raising than the soil quality of handling without the method for the invention.
Table 1 is through the soil organic matter content contrast table of different treatment
Fig. 1 and Fig. 2 be to use respectively the paddy rice that the method for the invention cultivates and be used to contrast without the conventional cultivation paddy rice of using the method for the invention in the total nitrogen content of the grain of rice and the contrast aspect the output per hectare, as can be seen, quality and the output of the paddy rice of handling through the present invention improve a lot more than the conventional cultivation paddy rice of contrast among the figure.
Description of drawings
Fig. 1 is the contrast through the paddy rice grain of rice total nitrogen content of different treatment.
Fig. 2 is the contrast through the paddy rice output per hectare of different treatment.
Fig. 3 is preparation technology's flow process of the present invention.
Embodiment
See also Fig. 3; the fixed nitrogen of the compound operation of blue-green algae-paddy rice of the present invention is original material with the method for solid carbon with Herba azollae imbricatae anabena, fixed nitrogen anabena and three kinds of fixed nitrogen blue-green algaes of wawter bloom anabena; through sterilisable chamber algae kind spread cultivation and booth Xiao Chi large-scale production after; be processed into the certain algae kind of water content, these three kinds of algae kinds that are mixed in proportion also are sprinkled upon among the rice field of pouring water equably.
Below in conjunction with embodiment the present invention is elaborated, this embodiment is that prerequisite has provided detailed embodiment and concrete operating process with the technical solution of the present invention, but protection scope of the present invention is not limited only to following embodiment.
Main raw and equipment: three kinds of fixed nitrogen blue-green algaes (Herba azollae imbricatae anabena, fixed nitrogen anabena, wawter bloom anabena), sieve, open container, stirring rod, baking oven, balance, booth Xiao Chi.
Concrete steps are:
1) is original material with Herba azollae imbricatae anabena, fixed nitrogen anabena and wawter bloom anabena, at first spreads cultivation that its processing condition are: light intensity 3000 ± 200Lx, 30 ± 2 ℃ of temperature through sterilisable chamber algae kind; The algae kind that sterilisable chamber is cultivated is seeded in large-scale production among the booth Xiao Chi (the long 2m of size * wide 0.5m * high 0.8m) then, 30 ± 2 ℃ of temperature, and natural light, every pond adds the algae kind 4g of water content 50%, cultured continuously 4 days; By sieve control water, fixed nitrogen anabena and Herba azollae imbricatae anabena are by 300 purpose sieves afterwards, and the wawter bloom anabena is gathered in the crops the wet algae of water content 50% by 50 purpose sieves.
2) that day of obtaining wet algae with the wet algaes of three kinds of fixed nitrogen blue-green algaes according to the Herba azollae imbricatae anabena: the fixed nitrogen anabena: wawter bloom anabena mass ratio is that 2:6:2 is blended in the container, and smashes with stirring rod.
3) the fixed nitrogen blue-green algae after will being mixed is sprinkled upon in the rice field of pouring water equably with the density of every square metre of 3g.
As calculated, the rice yield of putting the wet algae of fixed nitrogen blue-green algae in a suitable place to breed reaches the 15783.30kg/ hectare, and comparison is seen Fig. 2 according to exceeding 39.18%(); Grain of rice total nitrogen content reaches 8.03mg/g, and comparison is seen Fig. 1 according to many 9.20%(); Average every month newly-increased organic content of soil is 16.80g/kg, is 2.05 times (seeing Table 1) of contrast.
Main raw and equipment: three kinds of fixed nitrogen blue-green algaes (Herba azollae imbricatae anabena, fixed nitrogen anabena, wawter bloom anabena), sieve, open container, stirring rod, baking oven, balance, booth Xiao Chi.
The concrete steps of present embodiment are except that following processing condition, and all the other contents are identical with the foregoing description 1 with step process:
The long 1.5m of the little pool size of booth * wide 1m * high 1.2m, every pond adds the algae kind 5g of water content 50%, cultured continuously 5 days, by sieve control water, fixed nitrogen anabena and Herba azollae imbricatae anabena are by 250 purpose sieves, and the wawter bloom anabena is gathered in the crops the wet algae of water content 60% by 80 purpose sieves; The wet algae of three kinds of fixed nitrogen blue-green algaes is according to the Herba azollae imbricatae anabena: the fixed nitrogen anabena: wawter bloom anabena mass ratio is that 2.5:5:2.5 mixes; Fixed nitrogen blue-green algae after being mixed is sprinkled upon in the rice field of pouring water equably with the density of every square metre of 2g.
Main raw and equipment: three kinds of fixed nitrogen blue-green algaes (Herba azollae imbricatae anabena, fixed nitrogen anabena, wawter bloom anabena), sieve, open container, stirring rod, baking oven, balance, booth Xiao Chi.
The concrete steps of present embodiment are except that following processing condition, and all the other contents are identical with the foregoing description 1 with step process:
The long 1m of the little pool size of booth * wide 0.8m * high 1m, every pond adds the algae kind 2g of water content 50%, cultured continuously 3 days, by sieve control water, fixed nitrogen anabena and Herba azollae imbricatae anabena are by 200 purpose sieves, and the wawter bloom anabena is gathered in the crops the wet algae of water content 40% by 100 purpose sieves; The wet algae of three kinds of fixed nitrogen blue-green algaes is according to the Herba azollae imbricatae anabena: the fixed nitrogen anabena: wawter bloom anabena mass ratio is that 3:4:3 mixes; Fixed nitrogen blue-green algae after being mixed is sprinkled upon in the rice field of pouring water equably with the density of every square metre of 5g.
Main raw and equipment: three kinds of fixed nitrogen blue-green algaes (Herba azollae imbricatae anabena, fixed nitrogen anabena, wawter bloom anabena), sieve, open container, stirring rod, baking oven, balance, booth Xiao Chi, rice husk, pulverizer.
The concrete steps of present embodiment are except that following processing condition, and all the other contents are identical with the foregoing description 1 with step process:
The wet algae that obtains is placed 40 ℃ baking oven 8h oven dry, makes it water content and reach 10%, pulverize with pulverizer, with the dry algae powders of three kinds of fixed nitrogen blue-green algaes according to the Herba azollae imbricatae anabena: the fixed nitrogen anabena: wawter bloom anabena mass ratio is that 2:6:2 is mixed evenly; The dry algae powder of fixed nitrogen blue-green algae after will being mixed is again mixed in the rice husk of 7 times of quality, is sprinkled upon equably in the rice field of pouring water with the density of every square metre of 0.5g dry algae powder.
As calculated, the rice yield of putting dried algae in a suitable place to breed reaches the 14950.50kg/ hectare, and comparison is seen Fig. 2 according to exceeding 31.83%(); Grain of rice total nitrogen content reaches 8.04mg/g, and comparison is seen Fig. 1 according to many 9.33%(); Average every month newly-increased organic content of soil is 14.92g/kg, is 1.82 times (seeing Table 1) of contrast.
Embodiment 5
Main raw and equipment: three kinds of fixed nitrogen blue-green algaes (Herba azollae imbricatae anabena, fixed nitrogen anabena, wawter bloom anabena), sieve, open container, stirring rod, baking oven, balance, booth Xiao Chi, rice husk, pulverizer.
The concrete steps of present embodiment are except that following processing condition, and all the other contents are identical with the foregoing description 2 with step process:
The wet algae that obtains is placed 35 ℃ baking oven 8h oven dry, makes it water content and reach 12%, pulverize with pulverizer, with the dry algae powders of three kinds of fixed nitrogen blue-green algaes according to the Herba azollae imbricatae anabena: the fixed nitrogen anabena: wawter bloom anabena mass ratio is that 1:8:1 is mixed evenly; The dry algae powder of fixed nitrogen blue-green algae after will being mixed is again mixed in the rice husk of 10 times of quality, is sprinkled upon equably in the rice field of pouring water with the density of every square metre of 0.5g dry algae powder.
Main raw and equipment: three kinds of fixed nitrogen blue-green algaes (Herba azollae imbricatae anabena, fixed nitrogen anabena, wawter bloom anabena), sieve, open container, stirring rod, baking oven, balance, booth Xiao Chi, rice husk, pulverizer.
The concrete steps of present embodiment are except that following processing condition, and all the other contents are identical with the foregoing description 3 with step process:
Place 38 ℃ baking oven 8h to dry the wet algae that obtains, make it water content and reach 8%, pulverize with pulverizer, with the dry algae powders of three kinds of fixed nitrogen blue-green algaes according to the Herba azollae imbricatae anabena: the fixed nitrogen anabena: wawter bloom anabena mass ratio is that 1.5:7:1.5 is mixed evenly; The dry algae powder of fixed nitrogen blue-green algae after will being mixed is again mixed in the rice husk of 5 times of quality, is sprinkled upon equably in the rice field of pouring water with the density of every square metre of 0.5g dry algae powder.
Embodiment 7
Main raw and equipment: three kinds of fixed nitrogen blue-green algaes (Herba azollae imbricatae anabena, fixed nitrogen anabena, wawter bloom anabena), sieve, open container, stirring rod, baking oven, balance, booth Xiao Chi, rice husk, pulverizer.
The concrete steps of present embodiment are except that following processing condition, and all the other contents are identical with the foregoing description 4 with step process:
Place the fluorescent lamp of 50 watts of sterilisable chambers to descend dry 4 days the wet algae that obtains, make it water content and reach 8%, pulverize with pulverizer, with the dry algae powders of three kinds of fixed nitrogen blue-green algaes according to the Herba azollae imbricatae anabena: the fixed nitrogen anabena: wawter bloom anabena mass ratio is that 1:8:1 is mixed evenly.
Main raw and equipment: three kinds of fixed nitrogen blue-green algaes (Herba azollae imbricatae anabena, fixed nitrogen anabena, wawter bloom anabena), sieve, open container, stirring rod, baking oven, balance, booth Xiao Chi, rice husk, pulverizer.
The concrete steps of present embodiment are except that following processing condition, and all the other contents are identical with the foregoing description 5 with step process:
Place the fluorescent lamp of 30 watts of sterilisable chambers to descend dry 3 days the wet algae that obtains, make it water content and reach 12%, pulverize with pulverizer, with the dry algae powders of three kinds of fixed nitrogen blue-green algaes according to the Herba azollae imbricatae anabena: the fixed nitrogen anabena: wawter bloom anabena mass ratio is that 2:6:2 is mixed evenly.
Embodiment 9
Main raw and equipment: three kinds of fixed nitrogen blue-green algaes (Herba azollae imbricatae anabena, fixed nitrogen anabena, wawter bloom anabena), sieve, open container, stirring rod, baking oven, balance, booth Xiao Chi, rice husk, pulverizer.
The concrete steps of present embodiment are except that following processing condition, and all the other contents are identical with the foregoing description 6 with step process:
Place the fluorescent lamp of 40 watts of sterilisable chambers to descend dry 5 days the wet algae that obtains, make it water content and reach 10%, pulverize with pulverizer, with the dry algae powders of three kinds of fixed nitrogen blue-green algaes according to the Herba azollae imbricatae anabena: the fixed nitrogen anabena: wawter bloom anabena mass ratio is that 1.5:7:1.5 is mixed evenly.
Above embodiment is not a limitation of the present invention, can adjust proportioning of three kinds of fixed nitrogen blue-green algaes and the amount that put in a suitable place to breed in the rice field when specifically implementing.
The contriver has carried out a large amount of experiments, find out the optimum process parameter in the described technology, see also Fig. 1,2 and table 1, optimised process is as follows: the algae kind that sterilisable chamber is cultivated is seeded among the booth Xiao Chi (long 2 * wide by 0.5 * high 0.8m), the algae kind 4g of every pond water content 50%, cultured continuously 4 days, the fixed nitrogen anabena of volume production and Herba azollae imbricatae anabena are by 300 purpose sieves, the wawter bloom anabena is by 50 purpose sieves, the wet algae of results water content 50%, (Herba azollae imbricatae anabena: fixed nitrogen anabena: the wawter bloom anabena) 2:6:2 mixed and blended, and was sprinkled upon equably with every square metre of 3g in the rice field of pouring water by mass ratio with 3 kinds of wet algaes the same day; Perhaps blue-green algae is placed 40 ℃ baking oven to dry, make water content reach 10%, pulverize, press mass ratio 1:8:1, dry powder is mixed in the rice husk of 7 times of quality again, spill equably to the rice field of pouring water with every square metre of 5g with three kinds of dry algae powder mixings with pulverizer.
Claims (10)
1. the fixed nitrogen of the compound operation of blue-green algae-paddy rice and the method for carbon admittedly; it is characterized in that; with three kinds of fixed nitrogen blue-green algaes is original material; through sterilisable chamber algae kind spread cultivation and booth Xiao Chi large-scale production after; be processed into the certain algae kind of water content, these three kinds of algae kinds that are mixed in proportion also are sprinkled upon among the rice field of pouring water equably.
2. the method for the fixed nitrogen of the compound operation of blue-green algae-paddy rice according to claim 1 and solid carbon is characterized in that described three kinds of fixed nitrogen blue-green algaes are Herba azollae imbricatae anabena, fixed nitrogen anabena and wawter bloom anabena.
3. the method for the fixed nitrogen of the compound operation of blue-green algae-paddy rice according to claim 2 and solid carbon is characterized in that the concrete steps of described method are as follows:
1) be original material with Herba azollae imbricatae anabena, fixed nitrogen anabena and wawter bloom anabena, through sterilisable chamber algae kind spread cultivation and booth Xiao Chi large-scale production after, by sieve control water, make it water content 40%～60%;
2) same day of obtaining wet algae with three kinds of fixed nitrogen blue-green algaes according to the Herba azollae imbricatae anabena: the fixed nitrogen anabena: wawter bloom anabena mass ratio is that 2:6:2～3:4:3 mixes and blends;
3) the fixed nitrogen blue-green algae after will being mixed is sprinkled upon in the rice field of pouring water equably with the density of every square metre of 2～5g.
4. the method for the fixed nitrogen of the compound operation of blue-green algae-paddy rice according to claim 3 and solid carbon, it is characterized in that, the described same day of obtaining wet algae with three kinds of fixed nitrogen blue-green algaes according to the Herba azollae imbricatae anabena: the fixed nitrogen anabena: wawter bloom anabena mass ratio is that 2:6:2～3:4:3 mixes and blends and is meant, be blended in three kinds of wet algaes in the container that day of results blue-green algae, smashes with stirring rod.
5. the method for the fixed nitrogen of the compound operation of blue-green algae-paddy rice according to claim 2 and solid carbon is characterized in that the concrete steps of described method are as follows:
1) be original material with Herba azollae imbricatae anabena, fixed nitrogen anabena and wawter bloom anabena, through sterilisable chamber algae kind spread cultivation and booth Xiao Chi large-scale production after, by sieve control water, make it water content 40%～60%;
2) the wet algae that will obtain places 35～40 ℃ baking oven 8h, make it water content between 8%～12%, pulverize with pulverizer, with the dry algae powders of three kinds of fixed nitrogen blue-green algaes according to the Herba azollae imbricatae anabena: the fixed nitrogen anabena: wawter bloom anabena mass ratio is that 1:8:1～2:6:2 is mixed evenly;
3) dry algae powder of the fixed nitrogen blue-green algae after will being mixed is mixed in the rice husk of 5～10 times of quality, is sprinkled upon equably in the rice field of pouring water with the density of every square metre of 0.5g dry algae powder.
6. the method for the fixed nitrogen of the compound operation of blue-green algae-paddy rice according to claim 5 and solid carbon is characterized in that described step 2) the contents are as follows:
2) the wet algae that will obtain places the fluorescent lamp of 30～50 watts of sterilisable chambers to descend dry 3～5 days, make it water content between 8%～12%, pulverize with pulverizer, with the dry algae powders of three kinds of fixed nitrogen blue-green algaes according to the Herba azollae imbricatae anabena: the fixed nitrogen anabena: wawter bloom anabena mass ratio is that 1:8:1～2:6:2 is mixed evenly.
7. according to the fixed nitrogen of claim 5 or the compound operation of 6 described blue-green algae-paddy rice method with solid carbon, it is characterized in that, the dry algae powder of fixed nitrogen blue-green algae after described will being mixed is mixed in the rice husk of 5～10 times of quality and is meant, rice husk as the carrier that supports described dry algae powder, is sneaked into this dry algae powder in the rice husk of 5～10 times of quality.
8. according to the fixed nitrogen and the method for consolidating carbon of claim 3, the compound operation of 5 or 6 described blue-green algae-paddy rice, it is characterized in that the processing condition that described sterilisable chamber algae kind spreads cultivation are: light intensity 3000 ± 200Lx, 30 ± 2 ℃ of temperature.
9. according to the fixed nitrogen of claim 3, the compound operation of 5 or 6 described blue-green algae-paddy rice method with solid carbon; it is characterized in that; the processing condition of described booth Xiao Chi large-scale production are: Xiao Chi length * wide * high size 1～2m * 0.5～1m * 0.8～1.2m; 30 ± 2 ℃ of temperature; natural light; each Xiao Chi adds the algae kind 2～5g of water content 50%, cultured continuously 3～5 days.
10. according to the fixed nitrogen and the method for consolidating carbon of claim 3, the compound operation of 5 or 6 described blue-green algae-paddy rice, it is characterized in that the aperture of described sieve is 50～300 orders.
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| CN103907423A (en) * | 2014-03-21 | 2014-07-09 | 罗时词 | Cultivation method of chlorella organic rice |
| CN104604605A (en) * | 2015-01-08 | 2015-05-13 | 上海交通大学 | Composite control method for paddy field weeds |
| CN105985910A (en) * | 2015-03-05 | 2016-10-05 | 华东理工大学 | Novel method and process for carbon supplement during microalgae culture |
| CN106856921A (en) * | 2017-02-14 | 2017-06-20 | 中国水稻研究所 | A kind of utilization fine duckweed matches somebody with somebody the cultural method for applying regulation and control rice field nitrogen sustained release |
| CN107852884A (en) * | 2017-12-04 | 2018-03-30 | 中国科学院水生生物研究所 | A kind of method for improveing acidified soil using Azotica and lifting soil fertility |
| CN109220668A (en) * | 2017-11-28 | 2019-01-18 | 卢梅雅 | Azotica is improving the application in tuber crops yield |
| CN109220298A (en) * | 2017-11-28 | 2019-01-18 | 卢梅雅 | Activity In Anabaena Azotica is promoting growing of mulberry and is improving the application in mulberry tree leaf-making quantity |
| CN110122228A (en) * | 2019-06-03 | 2019-08-16 | 江苏省农业科学院 | A method of it reducing rice field ammonia volatilization and improves nitrogen fertilizer for paddy rice utilization rate |
| CN110205135A (en) * | 2019-07-08 | 2019-09-06 | 山东多芬农业有限公司 | A method of soil and fixed nitrogen are administered using cyanobacteria |
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| CN104604605A (en) * | 2015-01-08 | 2015-05-13 | 上海交通大学 | Composite control method for paddy field weeds |
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| CN105985910A (en) * | 2015-03-05 | 2016-10-05 | 华东理工大学 | Novel method and process for carbon supplement during microalgae culture |
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| CN109220668A (en) * | 2017-11-28 | 2019-01-18 | 卢梅雅 | Azotica is improving the application in tuber crops yield |
| CN109220298A (en) * | 2017-11-28 | 2019-01-18 | 卢梅雅 | Activity In Anabaena Azotica is promoting growing of mulberry and is improving the application in mulberry tree leaf-making quantity |
| CN107852884A (en) * | 2017-12-04 | 2018-03-30 | 中国科学院水生生物研究所 | A kind of method for improveing acidified soil using Azotica and lifting soil fertility |
| CN110122228A (en) * | 2019-06-03 | 2019-08-16 | 江苏省农业科学院 | A method of it reducing rice field ammonia volatilization and improves nitrogen fertilizer for paddy rice utilization rate |
| CN110205135A (en) * | 2019-07-08 | 2019-09-06 | 山东多芬农业有限公司 | A method of soil and fixed nitrogen are administered using cyanobacteria |
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