CN103211873B - Medicinal composition for treating hepatic fibrosis and preparation method thereof - Google Patents
Medicinal composition for treating hepatic fibrosis and preparation method thereof Download PDFInfo
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- CN103211873B CN103211873B CN201310159968.4A CN201310159968A CN103211873B CN 103211873 B CN103211873 B CN 103211873B CN 201310159968 A CN201310159968 A CN 201310159968A CN 103211873 B CN103211873 B CN 103211873B
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- marchantia
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Abstract
The invention discloses a Chinese medicament, which has the effects of clearing heat, promoting dieresis, protecting liver and resisting fibrosis and is used for anti-hepatic fibrosis treatment. The Chinese medicament comprises the following components in parts by mass: 3-30 parts of liverwort and 3-30 parts of Chinese knotweed herb. Pharmacological studies show that the medicament has the effects of protecting liver, reducing enzyme and resisting hepatic fibrosis, and is used for treating hepatic fibrosis clinically.
Description
Technical field
The present invention relates to a kind of Chinese patent medicine being used for the treatment of hepatic fibrosis, and production method.
Background technology
Hepatic fibrosis is commonly called as liver cirrhosis, refers to connective tissue paraplasm in liver caused by various virulence factor, causes the pathological process of diffusivity extracellular matrix excessive deposition in liver.It is not an independently disease, and many chronic hepatic diseases all can cause hepatic fibrosis.In China, how by drinking, viral hepatitis, bad life habits and bring out hepatic fibrosis, possess case load every year more than 3,000 ten thousand people.
Moss Marchantiaceae marchantia marchantia Marchantia polymorpha L., with all herbal medicine, the four seasons can adopt, and clean, using fresh herb or dry use.Have another name called ground Herba Spirodelae, a cloud etc., be Guangxi medical materials traditionally used in certain regions, have heat clearing away, draw out pus by applying a plaster to the affected part, granulation promoting effect, burn and scald is controlled in external, fracture, venom, sore and toxic, ecthyma, tinea.
Polygonaceae arsesmart Herb Polygoni Chinensis Polygunum chinense L., have another name called meal rattan, Linesless charcoal rattan, Herba Polygoni cymosi, river seven, wing land productivity, Linesless charcoal star, Linesless charcoal rattan, plain rice grass, plain rice rattan, letter meal rattan clearly, with all herbal medicine, the four seasons can adopt, cleaning and dry or using fresh herb, is Guangxi medical materials traditionally used in certain regions.Herb Polygoni Chinensis is sour, sweet, cold, returns liver, spleen channel, has heat-clearing and toxic substances removing, dampness removing disappears stagnant, blood-cooling itch-relieving, and improving acuity of vision and removing nebula effect, for dysentery, enteritis, dyspepsia, hepatitis, flu, tonsillitis, pharyngolaryngitis, diphtheria, pertussis, corneal nebula, colpitis mycotica, leucorrhea, mastitis, furuncle and phyma, children's's impetigo, eczema, venom.
In existing document, there is not yet the report of the prescription that to be used as medicine with marchantia and Herb Polygoni Chinensis.According to the inventors knowledge, this 2 taste medicine is only applied to some extent among the people, or clinically, uses when doctor opens magistral prescription.
Summary of the invention
Prescription of the present invention is made up of 2 taste medical materials, is Guangxi traditional herbal medicine, is respectively marchantia, Herb Polygoni Chinensis.The object of this invention is to provide a kind of Chinese medicine preparation that there is clearing away heat-damp and promoting diuresis, protect the liver anti-fine effect, be used for the treatment of hepatic fibrosis; Another object of the present invention is to provide the preparation method of this drug regimen.
Drug regimen of the present invention is made up of following component (mass parts): marchantia 3 ~ 30, and Herb Polygoni Chinensis 3 ~ 30.
The present invention preferably combines: marchantia 10, and Herb Polygoni Chinensis 12.
In order to said medicine combination is prepared as Chinese patent medicine, complete by following method: 1. marchantia 70% alcohol reflux; 2. Herb Polygoni Chinensis's decocting in water, water cooking liquid concentrates, and precipitate with ethanol, gets supernatant; 3. precipitate with ethanol supernatant and marchantia alcohol extract are merged; 4. be concentrated into thick paste, be roasted into dry cream; 5. dry cream breaks into fine powder; 6. add adjuvant, make required dosage form, as tablet, pill, capsule etc.; 7. also can directly by step 4. in thick paste, add suitable quantity of water and sugar, make oral liquid.
Compared with prior art, marked improvement of the present invention shows as:
1. adopt marchantia and Herb Polygoni Chinensis to form prescriptions of Chinese medicine first.
2. the medicine be made up of marchantia and Herb Polygoni Chinensis of Late Cambrian, has effect of anti hepatic fibrosis, can be used for the clinical treatment of hepatic fibrosis.
Detailed description of the invention
The following example is used for illustrating the present invention, is not any restriction to scope.
Embodiment one
Prescription: marchantia 1000g, Herb Polygoni Chinensis 1200g.
(1) marchantia 70% alcohol reflux, first time 15 times amount (v/w), 2 hours; Second time 8 times amount (v/w), 1 hour; Merge 2 alcohol extracts, filter, for subsequent use.
(2) Herb Polygoni Chinensis's water extraction, first time 12 times amount (v/w), 2 hours; Second time 8 times amount (v/w), 1 hour; Merge twice Aqueous extracts, filter, be evaporated to proportion 1.10 ~ 1.20 (80 DEG C), precipitate with ethanol process done by 95% ethanol adding 2 times amount, leaves standstill 72 hours, leaching supernatant.
(3) Herb Polygoni Chinensis's precipitate with ethanol supernatant and marchantia alcohol extract are merged, be evaporated to thick paste.Thick paste moves into pallet, and 80 ~ 100 DEG C are roasted into dry cream.
(4) dry cream breaks into fine powder, whole mistake 80 mesh sieve.
(5) add appropriate amount of starch, granulate with 85% ethanol water, be pressed into 900.
(6) usage and dosage: every day 3 times, each 3.
Embodiment two
Prescription: marchantia 1000g, Herb Polygoni Chinensis 1000g.
(1) marchantia 75% alcohol reflux, first time 12 times amount (v/w), 1 hour; Second time 8 times amount (v/w), 1 hour; Third time 8 times amount (v/w), 1 hour; Merge 3 alcohol extracts, filter, for subsequent use.
(2) Herb Polygoni Chinensis's water extraction, first time 15 times amount (v/w), 1.5 hours; Second time 10 times amount (v/w), 1 hour; Merge twice Aqueous extracts, filter, be evaporated to proportion 1.10 ~ 1.20 (80 DEG C), precipitate with ethanol process done by 95% ethanol adding 2.5 times amount, leaves standstill 60 hours, leaching supernatant.
(3) Herb Polygoni Chinensis's precipitate with ethanol supernatant and marchantia alcohol extract are merged, be evaporated to thick paste.Thick paste moves into pallet, and 80 ~ 100 DEG C are roasted into dry cream.
(4) dry cream breaks into fine powder, whole mistake 80 mesh sieve.
(5) add appropriate amount of starch, make binding agent with Mel, general ball, obtain 900 balls.
(6) usage and dosage: every day 3 times, each 3 balls.
Embodiment three
Prescription: marchantia 1000g, Herb Polygoni Chinensis 1500g.
(1) marchantia 65% alcohol reflux, first time 15 times amount (v/w), 1 hour; Second time 8 times amount (v/w), 1 hour; Third time 8 times amount (v/w), 1 hour; Merge 3 alcohol extracts, filter, for subsequent use.
(2) Herb Polygoni Chinensis's water extraction, first time 15 times amount (v/w), 1.5 hours; Second time 10 times amount (v/w), 1 hour; Merge twice Aqueous extracts, filter, be evaporated to proportion 1.10 ~ 1.20 (80 DEG C), precipitate with ethanol process done by 95% ethanol adding 2 times amount, leaves standstill 96 hours, leaching supernatant.
(3) Herb Polygoni Chinensis's precipitate with ethanol supernatant and marchantia alcohol extract are merged, be evaporated to thick paste.Thick paste moves into pallet, and 80 ~ 100 DEG C are roasted into dry cream.
(4) dry cream breaks into fine powder, whole mistake 80 mesh sieve.
(5) add appropriate amount of starch, granulate.
(6) granule incapsulates, and obtains 600.
(7) usage and dosage: every day 3 times, each 2.
Embodiment four
Prescription: marchantia 1000g, Herb Polygoni Chinensis 1200g.
(1) marchantia 70% alcohol reflux, first time 15 times amount (v/w), 2 hours; Second time 8 times amount (v/w), 1 hour; Merge 2 alcohol extracts, filter, for subsequent use.
(2) Herb Polygoni Chinensis's water extraction, first time 12 times amount (v/w), 2 hours; Second time 8 times amount (v/w), 1 hour; Merge twice Aqueous extracts, filter, be evaporated to proportion 1.10 ~ 1.20 (80 DEG C), precipitate with ethanol process done by 95% ethanol adding 2 times amount, leaves standstill 96 hours, leaching supernatant.
(3) Herb Polygoni Chinensis's precipitate with ethanol supernatant and marchantia alcohol extract are merged, be evaporated to thick paste.
(4) a small amount of water heating of thick paste melts, and adds 1800g white sugar, heating for dissolving, then continues to add water, be settled to 3000ml, fully mix, obtain oral liquid.
(5) usage and dosage: every day 3 times, each 10ml.
Pharmacological experiment
The tablet (for convenience of description, be denoted as " master slice ") getting gained in above-described embodiment one carries out following pharmacological experiment.
(1) to CCl
4the protective effect of induced Acute liver injury model:
Get mice 60, be divided into Normal group (distilled water 20ml/kg), CCl at random
4model control group (distilled water, 20ml/kg), bifendate matched group (0.1g/kg), the big-and-middle small dose group of ground master slice (1.2,0.6,0.3g/kg).Mice gavage every day (ig) administration 1 time, continuous 15d.After last administration 1h, except Normal group, each ip1%CCl
4peanut oil solution 10ml/kg.Get blood from mouse orbit venous plexus after 20h, the centrifugal 20min of 2500rpm/min, separation of serum, measure the activity of alanine aminopherase in serum (ALT) and aspartate aminotransferase (AST), the results are shown in Table 1.Bifendate group and ground master slice heavy dose group obviously reduce the activity (p < 0.01) of ALT, AST, middle dosage takes second place (p < 0.05), small dose group also has the trend of reduction, but not obvious (p > 0.05).
Table 1 couple CCl
4the impact of induced mice acute hepatic injury model ALT activity (
n=10)
Note: through t inspection, with CCl
4model control group compares, * * p < 0.01, * p < 0.05
(2) on the impact of Liver Fibrosis
Get rat, be divided into Normal group (distilled water 20ml/kg), CCl at random
4model control group (distilled water, 20ml/kg), CCl
4+ bifendate matched group (0.1g/kg), CCl
4the big-and-middle small dose group of+ground master slice (1.2,0.6,0.3g/kg).Gavage every day (ig) is administered once, continuous 45d; Except Normal group, every each animal ipCCl of 3d
40.3ml/100g (40% soybean oil solution), initial dose 0.5ml/100g.After last administration 24h, sacrificed by decapitation animal, gets hepatic tissue and measures hydroxyproline regulating liver-QI total lipid content, the results are shown in Table 2, table 3.Do hepatic tissue pathology section, after dyeing, the fibrosis of basis of microscopic observation liver, compares with model group simultaneously.
Table 2 on the impact of hydroxyproline content in rat liver tissue (
n=10)
Note: through t inspection, with CCl
4model control group compares, * * p < 0.01, * p < 0.05
Table 3 on the impact of total lipid content in rat liver tissue (
n=10)
Wang: through t inspection, with CCl
4model control group compares, * * p < 0.01, * p < 0.05
Table 2 and table 3 show, bifendate group and ground master slice heavy dose organize the hydroxyproline content and total lipid content (p < 0.01) that all can significantly reduce in hepatic tissue, and middle dosage group takes second place (p < 0.05).Check pathological section result shows, bifendate group, the hepatic tissue fibroplasia of the big or middle dosage group of master slice obviously reduce, have no severe fibrosis and occur; The comparatively middle dosage group of degree of hepatic fibrosis of ground master slice heavy dose group is light, and middle dosage group smaller dose group is light, show dose-effect relation.
Clinical practice
Drug regimen of the present invention, applies for many years clinically as doctor's magistral prescription; Used case shows, this medicine can delay and alleviate the fibrosis of liver.
Claims (1)
1. the purposes of Chinese medicine preparation in the medicine of preparation treatment hepatic fibrosis, it is characterized in that: in mass, crude drug proportion of composing is: marchantia 3 ~ 30, and Herb Polygoni Chinensis 3 ~ 30; The preparation method of Chinese medicine preparation is as follows: 1. marchantia 70% alcohol reflux; 2. Herb Polygoni Chinensis's decocting in water, water cooking liquid concentrates, and precipitate with ethanol, gets supernatant; 3. precipitate with ethanol supernatant and marchantia alcohol extract are merged; 4. be concentrated into thick paste, be roasted into dry cream; 5. dry cream is broken into fine powder; 6. add adjuvant, make peroral dosage form.
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| CN201310159968.4A CN103211873B (en) | 2013-05-03 | 2013-05-03 | Medicinal composition for treating hepatic fibrosis and preparation method thereof |
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| CN103211873B true CN103211873B (en) | 2015-01-07 |
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101185661A (en) * | 2006-03-10 | 2008-05-28 | 徐�明 | Technique for preparing liverwort extract and application of it in preparing medicine for treating liver cancer and hepatitis B |
| CN102697871A (en) * | 2012-06-06 | 2012-10-03 | 广西中医药大学 | Chinese herbal preparation with liver protecting and enzyme reducing effects and production method of same |
| CN102772737A (en) * | 2012-05-09 | 2012-11-14 | 黄淮学院 | Traditional Chinese medicine composition for treating liver cirrhosis and fibrosis and preparation method thereof |
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2013
- 2013-05-03 CN CN201310159968.4A patent/CN103211873B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101185661A (en) * | 2006-03-10 | 2008-05-28 | 徐�明 | Technique for preparing liverwort extract and application of it in preparing medicine for treating liver cancer and hepatitis B |
| CN102772737A (en) * | 2012-05-09 | 2012-11-14 | 黄淮学院 | Traditional Chinese medicine composition for treating liver cirrhosis and fibrosis and preparation method thereof |
| CN102697871A (en) * | 2012-06-06 | 2012-10-03 | 广西中医药大学 | Chinese herbal preparation with liver protecting and enzyme reducing effects and production method of same |
Non-Patent Citations (2)
| Title |
|---|
| 拳卷地钱中黄酮类化合物的分离纯化、结构表征及生物活性研究;朱华;《中国优秀博士学位论文全文数据库 工程科技I辑》;20041215(第04期);摘要 * |
| 火炭母醇提物对大鼠急性肝损伤的保护作用研究;高雅、朱华;《华西药学杂志》;20121231;第27卷(第3期);283-284 * |
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