CN103211873A - Medicinal composition for treating hepatic fibrosis and preparation method thereof - Google Patents

Medicinal composition for treating hepatic fibrosis and preparation method thereof Download PDF

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CN103211873A
CN103211873A CN2013101599684A CN201310159968A CN103211873A CN 103211873 A CN103211873 A CN 103211873A CN 2013101599684 A CN2013101599684 A CN 2013101599684A CN 201310159968 A CN201310159968 A CN 201310159968A CN 103211873 A CN103211873 A CN 103211873A
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marchantia
hepatic fibrosis
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ethanol
polygoni chinensis
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CN103211873B (en
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朱华
高雅
梁东艳
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Guangxi University of Chinese Medicine
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Guangxi University of Chinese Medicine
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Abstract

The invention discloses a Chinese medicament, which has the effects of clearing heat, promoting dieresis, protecting liver and resisting fibrosis and is used for anti-hepatic fibrosis treatment. The Chinese medicament comprises the following components in parts by mass: 3-30 parts of liverwort and 3-30 parts of Chinese knotweed herb. Pharmacological studies show that the medicament has the effects of protecting liver, reducing enzyme and resisting hepatic fibrosis, and is used for treating hepatic fibrosis clinically.

Description

A kind of drug regimen for the treatment of hepatic fibrosis and preparation method thereof
Technical field
The present invention relates to a kind of Chinese patent medicine that is used for the treatment of hepatic fibrosis, and production method.
Background technology
Hepatic fibrosis is commonly called as liver cirrhosis, is meant by connective tissue paraplasm in the liver due to the various virulence factors, causes the excessive sedimentary pathological process of diffusivity extracellular matrix in the liver.It is not an independently disease, and many chronic hepatic diseases all can cause hepatic fibrosis.In China, how by drink, viral hepatitis, bad life habits and bring out hepatic fibrosis, possess case load every year and surpass 3,000 ten thousand people.
Moss Marchantiaceae marchantia marchantia Marchantia polymorpha L., with all herbal medicine, the four seasons can adopt, and clean using fresh herb or dry usefulness.Having another name called ground Herba Spirodelae, a cloud etc., is the Guangxi medical materials traditionally used in certain regions, has heat clearing away, draw out pus by applying a plaster to the affected part, and the granulation promoting effect, burn and scald is controlled in external, fracture, venom, sore and toxic, ecthyma, tinea.
Polygonaceae arsesmart Herb Polygoni Chinensis Polygunum chinense L., have another name called meal rattan, Linesless charcoal rattan, Herba Polygoni cymosi, river seven, wing land productivity, Linesless charcoal star, Linesless charcoal rattan, plain rice grass, plain rice rattan, letter meal rattan clearly, with all herbal medicine, the four seasons can adopt, cleaning and dry or using fresh herb, is the Guangxi medical materials traditionally used in certain regions.Herb Polygoni Chinensis's acid, sweet, cold, return liver, spleen channel, have heat-clearing and toxic substances removing, dampness removing disappears stagnant, blood-cooling itch-relieving, and the improving acuity of vision and removing nebula effect is used for dysentery, enteritis, dyspepsia, hepatitis, flu, tonsillitis, pharyngolaryngitis, diphtheria, pertussis, corneal nebula, colpitis mycotica, leucorrhea, mastitis, furuncle and phyma, children's's impetigo, eczema, venom.
In existing document, Shang Weijian has the be used as medicine report of prescription with marchantia and Herb Polygoni Chinensis.Known to the inventor, this 2 flavor medicine is only used to some extent among the people, perhaps clinically, uses when the doctor opens magistral prescription.
Summary of the invention
Prescription of the present invention is made up of 2 flavor medical materials, is the Guangxi traditional herbal medicine, is respectively marchantia, Herb Polygoni Chinensis.The purpose of this invention is to provide a kind of Chinese medicine preparation that has clearing away heat-damp and promoting diuresis, protects the liver anti-fine effect, be used for the treatment of hepatic fibrosis; Another object of the present invention provides the preparation method of this drug regimen.
Drug regimen of the present invention is made up of following component (mass parts): marchantia 3~30, the Herb Polygoni Chinensis 3~30.
The present invention preferably makes up: marchantia 10, the Herb Polygoni Chinensis 12.
In order to be Chinese patent medicine, can finish by the following method: 1. marchantia 70% alcohol reflux the said medicine combined preparation; 2. Herb Polygoni Chinensis's decocting in water, water cooking liquid concentrates, and precipitate with ethanol is got supernatant; 3. precipitate with ethanol supernatant and marchantia alcohol extract are merged; 4. be concentrated into thick paste, be roasted into dried cream; 5. dried cream breaks into fine powder; 6. add adjuvant, make required dosage form, as tablet, pill, capsule etc.; 7. also can be directly with the thick paste of step in 4., add suitable quantity of water and sugar, make oral liquid.
Compared with prior art, marked improvement of the present invention shows as:
1. adopt marchantia and Herb Polygoni Chinensis to form prescriptions of Chinese medicine first.
2. find the medicine made by marchantia and Herb Polygoni Chinensis to have effect of anti hepatic fibrosis first, can be used for the clinical treatment of hepatic fibrosis.
The specific embodiment
The following example is used to illustrate the present invention, is not any restriction to protection domain of the present invention.
Embodiment one
Prescription: marchantia 1000g, Herb Polygoni Chinensis 1200g.
(1) marchantia 70% alcohol reflux, 15 times of amounts (v/w) for the first time, 2 hours; 8 times of amounts (v/w) for the second time, 1 hour; Merge 2 times alcohol extract, filter, standby.
(2) Herb Polygoni Chinensis uses water extraction, 12 times of amounts (v/w) for the first time, 2 hours; 8 times of amounts (v/w) for the second time, 1 hour; Merge the water extract twice, filter, be evaporated to proportion 1.10~1.20 (80 ℃), 95% ethanol that adds 2 times of amounts is done the precipitate with ethanol processing, leaves standstill the leaching supernatant 72 hours.
(3) Herb Polygoni Chinensis's precipitate with ethanol supernatant and marchantia alcohol extract are merged, be evaporated to thick paste.Thick paste moves into pallet, and 80~100 ℃ are roasted into dried cream.
(4) dried cream breaks into fine powder, whole mistake 80 mesh sieves.
(5) add appropriate amount of starch, granulate, be pressed into 900 with 85% ethanol water.
(6) usage and dosage: every day 3 times, each 3.
Embodiment two
Prescription: marchantia 1000g, Herb Polygoni Chinensis 1000g.
(1) marchantia 75% alcohol reflux, 12 times of amounts (v/w) for the first time, 1 hour; 8 times of amounts (v/w) for the second time, 1 hour; 8 times of amounts (v/w) for the third time, 1 hour; Merge 3 times alcohol extract, filter, standby.
(2) Herb Polygoni Chinensis uses water extraction, 15 times of amounts (v/w) for the first time, 1.5 hours; 10 times of amounts (v/w) for the second time, 1 hour; Merge the water extract twice, filter, be evaporated to proportion 1.10~1.20 (80 ℃), 95% ethanol that adds 2.5 times of amounts is done the precipitate with ethanol processing, leaves standstill the leaching supernatant 60 hours.
(3) Herb Polygoni Chinensis's precipitate with ethanol supernatant and marchantia alcohol extract are merged, be evaporated to thick paste.Thick paste moves into pallet, and 80~100 ℃ are roasted into dried cream.
(4) dried cream breaks into fine powder, whole mistake 80 mesh sieves.
(5) add appropriate amount of starch, make binding agent with Mel, general ball gets 900 balls.
(6) usage and dosage: every day 3 times, each 3 balls.
Embodiment three
Prescription: marchantia 1000g, Herb Polygoni Chinensis 1500g.
(1) marchantia 65% alcohol reflux, 15 times of amounts (v/w) for the first time, 1 hour; 8 times of amounts (v/w) for the second time, 1 hour; 8 times of amounts (v/w) for the third time, 1 hour; Merge 3 times alcohol extract, filter, standby.
(2) Herb Polygoni Chinensis uses water extraction, 15 times of amounts (v/w) for the first time, 1.5 hours; 10 times of amounts (v/w) for the second time, 1 hour; Merge the water extract twice, filter, be evaporated to proportion 1.10~1.20 (80 ℃), 95% ethanol that adds 2 times of amounts is done the precipitate with ethanol processing, leaves standstill the leaching supernatant 96 hours.
(3) Herb Polygoni Chinensis's precipitate with ethanol supernatant and marchantia alcohol extract are merged, be evaporated to thick paste.Thick paste moves into pallet, and 80~100 ℃ are roasted into dried cream.
(4) dried cream breaks into fine powder, whole mistake 80 mesh sieves.
(5) add appropriate amount of starch, granulate.
(6) granule incapsulates, and gets 600.
(7) usage and dosage: every day 3 times, each 2.
Embodiment four
Prescription: marchantia 1000g, Herb Polygoni Chinensis 1200g.
(1) marchantia 70% alcohol reflux, 15 times of amounts (v/w) for the first time, 2 hours; 8 times of amounts (v/w) for the second time, 1 hour; Merge 2 times alcohol extract, filter, standby.
(2) Herb Polygoni Chinensis uses water extraction, 12 times of amounts (v/w) for the first time, 2 hours; 8 times of amounts (v/w) for the second time, 1 hour; Merge the water extract twice, filter, be evaporated to proportion 1.10~1.20 (80 ℃), 95% ethanol that adds 2 times of amounts is done the precipitate with ethanol processing, leaves standstill the leaching supernatant 96 hours.
(3) Herb Polygoni Chinensis's precipitate with ethanol supernatant and marchantia alcohol extract are merged, be evaporated to thick paste.
(4) thick paste melts with the low amounts of water heating, adds the 1800g white sugar, and heating for dissolving continues to add water then, is settled to 3000ml, and fully mixing gets oral liquid.
(5) usage and dosage: every day 3 times, each 10ml.
Pharmacological experiment
The tablet (for convenience of description, note is done " master slice ") of getting gained in the foregoing description one carries out following pharmacological experiment.
(1) to CCl 4Due to the protective effect of acute liver damage model:
Get 60 of mices, be divided into normal control group (distilled water 20ml/kg), CCl at random 4Model control group (distilled water, 20ml/kg), bifendate matched group (0.1g/kg), the big-and-middle small dose group of ground master slice (1.2,0.6,0.3g/kg).Mice is irritated stomach (ig) administration 1 time every day, continuously 15d.Behind the last administration 1h, except that the normal control group, each ip1%CCl 4Peanut oil solution 10ml/kg.Get blood from the mouse orbit venous plexus behind the 20h, the centrifugal 20min of 2500rpm/min, separation of serum, the activity of alanine aminotransferase (ALT) and aspartate aminotransferase (AST) the results are shown in Table 1 in the mensuration serum.Bifendate group and the heavy dose of group of ground master slice obviously reduce the activity (p<0.01) of ALT, AST, and middle dosage takes second place (p<0.05), and small dose group also has the trend of reduction, but not obvious (p>0.05).
Table 1 couple CCl 4The active influence of induced mice acute liver damage model ALT (
Figure BSA00000887808900051
N=10)
Figure BSA00000887808900052
Annotate: through the t check, with CCl 4Model control group compares, * * p<0.01, * p<0.05
(2) to the influence of rat experiment hepatic fibrosis
Get rat, be divided into normal control group (distilled water 20ml/kg), CCl at random 4Model control group (distilled water, 20ml/kg), CCl 4+ bifendate matched group (0.1g/kg), CCl 4The big-and-middle small dose group of+ground master slice (1.2,0.6,0.3g/kg).Irritate stomach (ig) every day and be administered once, continuously 45d; Except that the normal control group, every each animal ipCCl of 3d 4(0.3ml/100g 40% soybean oil solution), initial dose 0.5ml/100g.Behind last administration 24h, the sacrificed by decapitation animal is got hepatic tissue and measures hydroxyproline regulating liver-QI total lipid content, the results are shown in Table 2, table 3.Do the hepatic tissue pathology section simultaneously, dyeing back microscopically is observed the fibrosis of liver, compares with model group.
The influence of hydroxyproline content in the table 2 pair rat liver tissue (
Figure BSA00000887808900053
N=10)
Figure BSA00000887808900054
Annotate: through the t check, with CCl 4Model control group compares, * * p<0.01, * p<0.05
The influence of total lipid content in the table 3 pair rat liver tissue (
Figure BSA00000887808900061
N=10)
Figure BSA00000887808900062
Wang: through the t check, with CCl 4Model control group compares, * * p<0.01, * p<0.05
Table 2 and table 3 show, bifendate group and the heavy dose of group of ground master slice all can significantly reduce hydroxyproline content and the total lipid content (p<0.01) in the hepatic tissue, middle dosage group take second place (p<0.05).The check pathological section result shows, the bifendate group, the hepatic tissue fibroplasia of the big or middle dosage group of master slice obviously reduce, do not see that the severe fibrosis takes place; The middle dosage group of degree of hepatic fibrosis of the heavy dose of group of ground master slice is light, and middle dosage group smaller dose group is light, show dose-effect relation.
Clinical practice
Drug regimen of the present invention is used for many years clinically as doctor's magistral prescription; Used case shows that this medicine can delay and alleviate the fibrosis of liver.

Claims (3)

1. pharmaceutical composition that is used for the treatment of hepatic fibrosis, it is characterized in that: in mass, the crude drug proportion of composing is: marchantia 3~30, the Herb Polygoni Chinensis 3~30.
2. pharmaceutical composition according to claim 1 is characterized in that: in mass, described crude drug proportion of composing comprises: marchantia 10, scorching charcoal mother 12.
3. pharmaceutical composition according to claim 1, the method that its preparation is become the medicine that is used for the treatment of hepatic fibrosis is as follows: 1. marchantia 70% alcohol reflux; 2. Herb Polygoni Chinensis's decocting in water, water cooking liquid concentrates, and precipitate with ethanol is got supernatant; 3. precipitate with ethanol supernatant and marchantia alcohol extract are merged; 4. be concentrated into thick paste, be roasted into dried cream; 5. dried cream is broken into fine powder; 6. add adjuvant, make peroral dosage form.
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Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101185661A (en) * 2006-03-10 2008-05-28 徐�明 Technique for preparing liverwort extract and application of it in preparing medicine for treating liver cancer and hepatitis B
CN102697871A (en) * 2012-06-06 2012-10-03 广西中医药大学 Chinese herbal preparation with liver protecting and enzyme reducing effects and production method of same
CN102772737A (en) * 2012-05-09 2012-11-14 黄淮学院 Traditional Chinese medicine composition for treating liver cirrhosis and fibrosis and preparation method thereof

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101185661A (en) * 2006-03-10 2008-05-28 徐�明 Technique for preparing liverwort extract and application of it in preparing medicine for treating liver cancer and hepatitis B
CN102772737A (en) * 2012-05-09 2012-11-14 黄淮学院 Traditional Chinese medicine composition for treating liver cirrhosis and fibrosis and preparation method thereof
CN102697871A (en) * 2012-06-06 2012-10-03 广西中医药大学 Chinese herbal preparation with liver protecting and enzyme reducing effects and production method of same

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
朱华: "拳卷地钱中黄酮类化合物的分离纯化、结构表征及生物活性研究", 《中国优秀博士学位论文全文数据库 工程科技I辑》 *
高雅、朱华: "火炭母醇提物对大鼠急性肝损伤的保护作用研究", 《华西药学杂志》 *

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