CN102697871A - Chinese herbal preparation with liver protecting and enzyme reducing effects and production method of same - Google Patents
Chinese herbal preparation with liver protecting and enzyme reducing effects and production method of same Download PDFInfo
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- CN102697871A CN102697871A CN2012101839927A CN201210183992A CN102697871A CN 102697871 A CN102697871 A CN 102697871A CN 2012101839927 A CN2012101839927 A CN 2012101839927A CN 201210183992 A CN201210183992 A CN 201210183992A CN 102697871 A CN102697871 A CN 102697871A
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Abstract
The invention belongs to the field of traditional Chinese medicine and relates to Chinese herbal preparation with hepatitis B virus resisting, liver protecting and enzyme reducing effects, and particularly relates to a Chinese herbal preparation which is made of extractive of Chinese knotweed (herb of polygonum Chinense L); and research results show that the Chinese herbal preparation can inhibit the secretion of HBsAg and HBeAg, and can remarkably lower blood serum ALT and MDA of an acute liver injured rat caused by carbon tetrachloride, and favorable liver protecting and enzyme reducing effects are displayed.
Description
Technical field
The invention belongs to tcm field, relating to Herb Polygoni Chinensis's (herb of polygonaceae plant Herb Polygoni Chinensis Polygonum chinenseL.) is the method for feedstock production Chinese medicine preparation, and the anti-hepatitis virus of this Chinese medicine preparation and the liver protecting and ALT lowering results of pharmacodynamic test.
Background technology
The Herb Polygoni Chinensis is the herb of polygonaceae plant Herb Polygoni Chinensis Polygonum chinense L..Its acrid in the mouth, hardship, cool in nature, poisonous, have clearing away heat-damp and promoting diuresis, removing pathogenic heat from blood and toxic substance from the body, suppressing the hyperactive liver makes eye bright, the effect of relaxing muscles and tendons to promote blood circulation; Cure mainly dysentery, have loose bowels, laryngopharynx swelling and pain, diphtheria; Pertussis, hepatitis, leukorrhagia, carbuncle; Eczema, corneal nebula, traumatic injury etc. [the herbal editorial board of State Administration of Traditional Chinese Medicine China. China's book on Chinese herbal medicine. the 2nd volume. Shanghai: Shanghai science tech publishing house, 1999:647-649].Herb Polygoni Chinensis's herb resource is abundant, and China central and east and southwestern each province all produce, and is one of Chinese herbal medicine commonly used among the people.
Relevant Herb Polygoni Chinensis's pharmacological action has the few of research bibliographical information now.Mainly contain: antibacterial action [Hu Haibin. the research of " Resistant strain " Chinese herbal medicine screening test. Guangdong College of Medical Pharmacy's journal; 1990; 6 (1): 40-41], antitumor [screening study of .50 kind Guangxi such as Wei Jinyu Chinese herbal medicine commonly used, strong medicine antitumor action. Guangxi university of TCM journal, 2003,6 (4): 3-7] and antivirus action [experimentation of .60 kind Chinese herbal medicine anti-hepatitis B virus such as Zhang Zheng. journal of Beijing Medical University; 1988,3 (20): 211-213].
To on April 26th, 2012, disclosed, relevant with Herb Polygoni Chinensis patent documentation amounted to 45, and wherein patent of invention is 44, all is Chinese traditional compound medicine.With Herb Polygoni Chinensis is the Chinese medicine preparation of folk prescription exploitation, and Shang Weijian has the patent documentation report.
In sum, the present invention has sufficient novelty, creativeness and practicality.
Summary of the invention
The inventor prepares a kind of ethanol extraction position with function for protecting liver and reducing enzyme activity from the Herb Polygoni Chinensis, and this effective site is prepared into Chinese medicine preparation, and this does not see at home as yet has the bibliographical information mistake.
Technical scheme of the present invention has been addressed from the Herb Polygoni Chinensis method of extracting the effective site with function for protecting liver and reducing enzyme activity, this effective site is prepared into the method for Chinese medicine preparation and this Chinese medicine preparation is carried out zooperal method of the liver protecting and ALT lowering and result.
The method for distilling of Herb Polygoni Chinensis's the liver protecting and ALT lowering effective site, Shang Weijian has bibliographical information.The inventor finds that through scientific research the effective ingredient of Herb Polygoni Chinensis's the liver protecting and ALT lowering is dissolved in the ethanol more than 65%, therefore, adopts 65%～95% ethanol water reflux, extract, in the invention.Because of containing a large amount of pigments and chlorophyll in the alcohol extract, the present invention adopts D
301Resin removes.The effective site that extraction obtains can be prepared into and supply the oral traditional Chinese medicine preparation, and like tablet, oral liquid, granule etc., the method for making of the corresponding dosage form of its method for making and document record is general.The part by weight scope of effective site and pharmaceutic adjuvant is following: contain Herb Polygoni Chinensis's extract 0.1%～99.9% in the preparation, surplus is a medicament molding adjuvant.
Compared with prior art, the present invention's substantive distinguishing features and obvious improvement of giving prominence to is: disclose the method for from the Herb Polygoni Chinensis, extracting the liver protecting and ALT lowering effective site first, this effective site is prepared into the method for tablet, oral liquid, granule and this Chinese medicine preparation is carried out zooperal method of the liver protecting and ALT lowering and result.
The specific embodiment
The following example is used to illustrate the present invention, is not any restriction to protection domain of the present invention.
One, be the examples of implementation that extract the liver protecting and ALT lowering effective site below:
Extract embodiment one: Herb Polygoni Chinensis's herb, dry, break into coarse powder, measure 70% ethanol water reflux, extract, 2 times, each 2 hours with 12 times; Merge alcohol extract, filter; Filtrating is passed through D
301Macroporous resin column (resin demand is 3 times of medical material weight) is collected effluent, be evaporated to rare cream (medical material: concentrated solution=2: 1, W/V).
Extract embodiment two: Herb Polygoni Chinensis's herb, dry, break into coarse powder, measure 85% ethanol water reflux, extract, 2 times, each 2 hours with 15 times; Merge alcohol extract, filter; Filtrating is passed through D
301Macroporous resin column (resin demand is 3 times of medical material weight) is collected effluent, and (medical material: concentrated solution=4: 1, W/V), the thick paste oven dry breaks into fine powder (60-80 order) to be evaporated to thick paste.
Two, be the examples of implementation that effective site are prepared into medicine below:
Pharmaceutical preparation embodiment one: the yield by effective site is calculated, and gets the dried cream fine powder of the effective site that is equivalent to 1000g Herb Polygoni Chinensis's crude drug, nearly weighs 24g, adds starch to total amount 100g, granulates, and is pressed into tablet, the heavy 0.3g of plain sheet, and every contains Herb Polygoni Chinensis's crude drug 3g.
Pharmaceutical preparation embodiment two: the yield by effective site is calculated, and gets the dried cream fine powder of the effective site that is equivalent to 1000g Herb Polygoni Chinensis's crude drug, nearly weighs 35g,, granulates to total amount 500g with Icing Sugar, gets granule, packing, and every parcel 5g, every parcel contain Herb Polygoni Chinensis's crude drug 10g.
Pharmaceutical preparation embodiment three: the yield by effective site is calculated, and gets the rare cream of the effective site that is equivalent to 1000g Herb Polygoni Chinensis's crude drug, and the about 500ml of volume is with white sugar 1200g; Heating for dissolving adds water at last to 2000ml, filters, and gets oral liquid; Packing, every 10ml, every contains Herb Polygoni Chinensis's crude drug 5g.
Three, be pharmacological action test method and result below:
The protective effect of a pair of acute liver damage of pharmacological evaluation
1.1 divide into groups and administration
The tablet (below be called " Linesless charcoal master slice ") of getting " pharmaceutical preparation embodiment one " gained is experimental subject.
Get 25 of SPF level healthy SD rats, the male and female dual-purpose, body weight is 200+20g, after laboratory rearing is stablized 2d, is divided into totally 5 groups of blank group, model control group, positive controls, Linesless charcoal master slice high dose group and Linesless charcoal master slice low dose group at random, 5 every group.Each organizes equal gastric infusion, every day 1 time, 7d continuously.Linesless charcoal master slice high dose group and low dose group are irritated stomach dosage and are respectively 2.0gkg
-1, 1.0gkg
-1(pressing the crude drug amount calculates), positive controls bifendate dosage is 0.05gkg
-1, blank group and model group give the equal-volume distilled water.
1.2 modeling and drawing materials
Behind the last administration 1h, blank group lumbar injection equal-volume Oleum Arachidis hypogaeae semen, all the other each groups subcutaneous injection 60% carbon tetrachloride Oleum Arachidis hypogaeae semen are respectively dissolved 5mLkg
-1, after water 24h is can't help in fasting, to extract eyeball and get blood, blood is placed 1h, 3000rmin
-1Centrifugal 3min gets upper serum, preserves in-4 ℃ of refrigerators, supplies the mensuration of biochemical indicators such as ALT, AST, MDA, SOD; It is fixing in 4% neutral buffered formalin solution to get hepatomegaly leaf texture simultaneously, supplies section to use.
1.3 date processing and statistical analysis
The experiments data are all represented with "
"; To relatively carrying out the independent sample t check between group, add up with the Excel2003 statistical software.
1.4 result
1.4.1 serum biochemistry index
Compare with the blank group, model group serum alt, AST and MDA activity all significantly raise, and SOD is active significantly to be reduced, and the modelling success is described; Compare with model group, Linesless charcoal master slice high and low dose group Serum ALT and MDA activity all obviously reduce than model group, and SOD all obviously raises than model group, and the active nothing of AST obviously changes.The serum biochemistry index is the result show, the Linesless charcoal master slice causes rats'liver damage to carbon tetrachloride and has protective effect, and prompting Linesless charcoal master slice possibly be through free radical resisting to hepatocellular protection, suppresses lipid peroxidation mechanism and plays a role.See table 1.
Table 1 Linesless charcoal master slice causes the influence of rats'liver damage serum biochemistry index to carbon tetrachloride
*: compare P＜0.05, * *: compare P＜0.01 with model group with model group.
1.4.2 histopathology is observed
The histopathologic slide microscopically is observed visible, blank group hepatic tissue lobules of liver clear in structure, hepatic cords is the center with the central vein, to around be radial, hepatocyte is a polygon, nuclear greatly, nuclear membrane is clear, kernel is obvious; The model group liver tissue injury is serious, and the lobules of liver structural fuzzy is unclear, and hepatocyte extensive edema, cavity shape have point-like and piecemeal necrosis in the lobule, inflammatory cell infiltrations such as companion's neutrophilic granulocyte, mononuclear cell, and pathological changes is distributed as the master with the ring central vein; Positive controls is the slight hepatic cell edema around central vein, and a small amount of little cavity shape pathological changes is only arranged; Linesless charcoal master slice high and low dose group edema degree is slight, and little cavity shape pathological changes is arranged, and does not have obvious tissue necrosis.The result of histopathologic slide shows that the Linesless charcoal master slice has bigger improvement to the hepatic necrosis degree that carbon tetrachloride causes the rats'liver damage.(figure slightly)
1.5 conclusion
Hepatic injury has the good protection effect to Herb Polygoni Chinensis's ethanol extract to rat acute.
Pharmacological evaluation two external anti-hepatitis virus
2.1 the preparation of reagent
Contain the preparation of Herb Polygoni Chinensis's extract test solution: get the dried cream 1g of " extracting embodiment two " gained, add dimethyl sulfoxide 50mL, configuration concentration is 0.02gmL
-1Medicinal liquid, filter with the micropore filter of 0.22 μ m under the gnotobasis, the reuse cell culture fluid is mixed with 16,8,4,2,1 respectively, 0.5mgmL
-1Deng the medicinal liquid of 6 variable concentrations, place 4 ℃ of refrigerators to preserve, for use.
The preparation of PBS PBS: get the PBS phosphate-buffered salt, be mixed with the buffer solution of 0.01N (pH=7.2～7.4) with ultra-pure water, under gnotobasis, using diameter is that the micropore filter of 0.22 μ m filters, and places 4 ℃ of refrigerators to preserve, for use.
The preparation of Digestive system: be mixed with 0.25% trypsin solution with the PBS PBS, the micropore filter with 0.22 μ m under gnotobasis filters, and places 4 ℃ of refrigerators to preserve, and is for use.
2.2HepG2.2.15 the recovery of cell strain and cultivation
The HepG2.2.15 cell that liquid nitrogen freezing is preserved melts in the back suction centrifuge tube in 37 ℃ of water-baths, adds the culture fluid of 10 times of amounts, mixing, 1000rmin
-1Low-speed centrifugal is removed supernatant, cleans once with culture fluid again.Then cell strain is seeded in the culture bottle, puts in the cell culture incubator at 37 ℃, 5%C0
2Environment is growth down, and the per 1～3d of visual cell growing state changes a culture fluid.
In the culture bottle that covers with HepG 2.2.15 cell, remove culture fluid, add 0.25% pancreatin, 37 ℃ of digestion 3～4min add culture fluid and blow and beat into single cell suspension gently, go down to posterity at 1: 3.Treat cell cover with after digestion once more, with behind the cell counting count board counting, adding culture fluid, to be diluted to concentration be 1 * 10
4Individual mL
-1Cell suspension, be inoculated in the 96 porocyte culture plates every hole 180 μ L, 37 ℃, 5%C0
2Cultivate 24h, cell attachment experimentizes after growing into monolayer.
2.3 the toxic detection of medicine pair cell
Mtt assay with reference to Mosmann foundation
[13]Detection of drugs is to the inhibitory action of HepG2.2.15 cell growth.After cell attachment grows into monolayer, be provided with and add medicinal liquid 20 μ L respectively, make in the culture fluid hole that final concentration is 1.6,0.8,0.4,0.2,0.1,0.05,0.025mgL
-1Deng 7 Concentraton gradient groups, establish the cell matched group that does not add medicine simultaneously, every group is repeated 5 holes.Behind the drug effect 72h, each hole adds MTT 20 μ L and continues to cultivate 4h.The supernatant that inclines adds PBS buffer solution and cleans 2 times, is inverted to clap gently in the absorbent paper and does, and guarantees the noresidue that exhausts.Every hole adds dimethyl sulfoxide 150 μ l, and 5min vibrates on the agitator.Light absorption value (OD) with ELIASA every hole of mensuration at the 490nm place calculates cell-damaging rate (seeing formula 1), and utilization medicine inhibition concentration software for calculation 1.0.0 version (LOGIT method) is calculated the poisonous concentration TC of half of medicine
50
2.4 antigen inhibitory action experiment
Experiment component experiment drug group and cell matched group.The HepG2.2.15 cell is processed single cell suspension after digestion, regulate cell concentration to 1 * 10
4Individual mL
-1, inoculate 96 well culture plates, every hole 180 μ L, 37 ℃, 5%C0
2Cultivate 24h, cell experimentizes after growing up to monolayer.According to the drug toxicity experimental result, the concentration of not having overt toxicity with pair cell is initial concentration, the medicine gradient become surely 4 kinds of final concentrations promptly 0.4,0.2,0.1,0.05mgmL
-1Every concentration of every kind of medicine adds 3 holes, establishes not dosing cell simultaneously and contrasts 3 holes and culture fluid blank 3 holes, behind the drug effect 72h, collects each porocyte supernatant and places 96 well culture plates, and-20 ℃ freezing subsequent use.Adopt HBsAg, HBeAg content in the ELISA method antigen diagnose reagent kit operation detection supernatant, calculate medicine to antigenic inhibition percentage rate (the same destructive rate of method), and calculate medium effective concentration IC
50(the same TC of method
50, the LOGIT method) and therapeutic index (TI), see formula 2:
TI=TC
50/ IC
50(formula 2)
2.5 date processing and statistical analysis
Mtt assay and ELISA method experimental data all with "
" expression, are added up with Excel 2003 statistical softwares.
2.6 result
2.6.1 toxic action to the HepG2.2.15 cell
Record the TC of Herb Polygoni Chinensis's alcohol extract with mtt assay to the HepG2.2.15 cell strain
50Be 1.322mgmL
-1The foundation that this result selects as the experimental drug substrate concentration.Inverted microscope is observed down, and visible liquor strength is 1.6,0.8mgmL
-1The time pair cell overt toxicity is arranged, the dead cell of the adherent minimizing of visible cell, shrinkage, suspension increases; Medicinal liquid 0.4mgmL
-1Following concentration is not all seen overt toxicity, and cell growth state is good, and survival rate is higher, and is similar with the cellular control unit existing state.Therefore, adopt 0.4mgmL
-1Following concentration is carried out HepG2.2.15 cell HBsAg, the excretory influence experiment of HBeAg.The result sees table 2.
The cytotoxicity of table 2 Herb Polygoni Chinensis ethanol extract
2.6.2 to HepG2.2.15 cell HBsAg, the excretory effect of HBeAg
Measure the result show the Herb Polygoni Chinensis in the HepG2.2.15 cell strain is cultivated behind the 72h secretion to HBsAg, HBeAg certain inhibitory action is arranged, and along with the increase of Herb Polygoni Chinensis's concentration, its inhibitory action strengthens, and is certain dose-effect relationship.Generally estimate the potential applicability in clinical practice of medicine with the TI value, TI＞2 are effective low toxicity, and 1＜TI＜2 are poisonous for effectively, and TI＜1 is a toxic action.Medicine is at the IC of HBsAg, HBeAg
50And the TI value, see table 3, table 4.
Table 3 Herb Polygoni Chinensis ethanol extract is in the HepG2.2.15 cell culture
The inhibitory action (
n=3) of secretion HBsAg and HBeAg
*: compare P＜0.05, * *: compare P＜0.01 with the cell matched group with the cell matched group.
The evaluating drug effect result of the anti-HBV of table 4 Herb Polygoni Chinensis ethanol extract
2.7 conclusion
The Herb Polygoni Chinensis is external to have certain inhibitory action to HepG2.2.15 emiocytosis HBsAg, HBeAg.
Claims (3)
1. Chinese medicine preparation, it is characterized in that: by weight, Herb Polygoni Chinensis's extract shared ratio in preparation is 0.5%～99.5%, and surplus is a medicament molding adjuvant.
2. Chinese medicine preparation according to claim 1, it is characterized in that: method for preparing is following: 1. do Herb Polygoni Chinensis's coarse powder, use concentration is 65%～95% ethanol water reflux, extract; 2. alcohol extract flows through D
301Macroporous resin column to remove pigment and chlorophyll, is collected effluent; 3. effluent reclaims solvent to rare cream; 4. the method for preparing of preparation has 2 kinds: first kind: add the white sand Icing Sugar in rare cream, and dissolving, mixing is processed oral liquid; Second kind: rare cream is further processed dried cream, and dried cream breaks into behind the fine powder and the figuration auxiliary materials and mixing, processes tablet, granule.
3. Chinese medicine preparation according to claim 1 is characterized in that: have function for protecting liver and reducing enzyme activity.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103211873A (en) * | 2013-05-03 | 2013-07-24 | 广西中医药大学 | Medicinal composition for treating hepatic fibrosis and preparation method thereof |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2001072583A (en) * | 1999-09-07 | 2001-03-21 | Sunstar Inc | Prophylactic or therapeutic composition for hyperlipemia |
| US20050042314A1 (en) * | 2003-08-22 | 2005-02-24 | National Yang-Ming University | Extracts of Polygonum multiflorum Thunb., and preparation process and uses of the same |
| CN1672719A (en) * | 2004-03-24 | 2005-09-28 | 吕圭源 | Application of fleece flower root in preparing medicine for treating fatty liver |
| CN1823941A (en) * | 2005-12-29 | 2006-08-30 | 唐素筠 | Xianxiang medicine for treating hepatitis B and its production method |
| CN101041029A (en) * | 2007-04-24 | 2007-09-26 | 罗顺卿 | Chinese traditional medicine for curing hepatitis B |
| CN101810607A (en) * | 2009-02-25 | 2010-08-25 | 财团法人工业技术研究院 | Hepatitis C resistant plant extracts composition |
-
2012
- 2012-06-06 CN CN201210183992.7A patent/CN102697871B/en active Active
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2001072583A (en) * | 1999-09-07 | 2001-03-21 | Sunstar Inc | Prophylactic or therapeutic composition for hyperlipemia |
| US20050042314A1 (en) * | 2003-08-22 | 2005-02-24 | National Yang-Ming University | Extracts of Polygonum multiflorum Thunb., and preparation process and uses of the same |
| CN1672719A (en) * | 2004-03-24 | 2005-09-28 | 吕圭源 | Application of fleece flower root in preparing medicine for treating fatty liver |
| CN1823941A (en) * | 2005-12-29 | 2006-08-30 | 唐素筠 | Xianxiang medicine for treating hepatitis B and its production method |
| CN101041029A (en) * | 2007-04-24 | 2007-09-26 | 罗顺卿 | Chinese traditional medicine for curing hepatitis B |
| CN101810607A (en) * | 2009-02-25 | 2010-08-25 | 财团法人工业技术研究院 | Hepatitis C resistant plant extracts composition |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103211873A (en) * | 2013-05-03 | 2013-07-24 | 广西中医药大学 | Medicinal composition for treating hepatic fibrosis and preparation method thereof |
| CN103211873B (en) * | 2013-05-03 | 2015-01-07 | 广西中医药大学 | Medicinal composition for treating hepatic fibrosis and preparation method thereof |
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