CN103417906A - Applications of compound mylabris traditional Chinese medicine composition and preparations thereof in preparing drugs for curing depression - Google Patents
Applications of compound mylabris traditional Chinese medicine composition and preparations thereof in preparing drugs for curing depression Download PDFInfo
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Abstract
The invention relates to applications of compound mylabris traditional Chinese medicine composition and preparations thereof in preparing drugs for curing depression, especially the drugs for curing tumor related depression. The compound mylabris traditional Chinese medicine composition is composed of eleven medicines such as mylabris, gen-seng, radix astragali, acanthopanax, sparganium, barbat skullcap, and the like. The compound mylabris preparation is prepared by adding corresponding auxiliary materials to the traditional Chinese medicine composition through conventional methods. The invention provides a novel drug for curing depression, and enlarges the clinical application of compound mylabris preparations.
Description
Technical field
The present invention relates to cantharides Chinese medicine composition and preparation thereof the new purposes in preparing the Cure of depression medicine, especially the purposes in preparation treatment tumor patient depression medicine.
Technical background
Depression, belong to the affective disorder disease, take of being caused by a variety of causes depression is cardinal symptom one group of mental maladjustment or affective disorder, clinical manifestation be depressed, interest goes down, self evaluation is too low, disheartened to future, be reluctant initiatively and people's contacts etc.Often, with corresponding thinking and behavior change, some patients were there will be hallucination and vain hope, and severe patient, with suicide, has a strong impact on people's quality of life.Majority of cases has the tendency of outbreak repeatedly, and the great majority that at every turn show effect can be alleviated, and part can have residual symptoms or transfer to chronic.
Depression has at least 10% patient maniac access can occur, now should be diagnosed as the two-phase obstacle.The depression that we often say in addition, refer to major depression (major depression) clinically in fact, in the crowd, has 16% people affected by it in certain period in all one's life.Suffer from depression except paying serious emotion and social costs, economic cost is also huge.According to World Health Organization's statistics, depression has become the large illness in the world the 4th, expects the year two thousand twenty, may become the second largest disease that is only second to coronary heart disease.
Along with the quickening of social life rhythm, the increase of operating pressure, the sickness rate of depression is ascendant trend year by year, and the early diagnosis of depression and treatment, more and more come into one's own.
In the middle of patients with depression, a very special colony merited attention is arranged, that is exactly the depression problem of tumor patient.Studies confirm that, most of tumor patients exist the unhealthy emotion reaction, wherein common with depressive symptom, be depressed (the cancer related depression of cancer-related, CRD), the patient that causes who refers to malignant tumor is diagnosed, produces in the process such as treatment and complication processing thereof loses the pathologic emotional response of individual spirit normality.On the one hand, the symptom of patients with depression is similar to some tumor patient, comprising not feeling like a meal, weight loss, insomnia, hebetude, energy forfeiture etc.On the other hand, depressive disorder again with the malignant tumor height correlation, both are sick large percentages altogether, malignant tumor patient occurs that depressive disorder can be caused by disease itself or chemotherapeutics, or the psychoreaction of deformity due to tumor again.According to epidemiological study, find, suffering from the depressed sickness rate of cancer-related in tumor patient is 18%～50%, what may have depressive symptom is 85.39%, and the patients undergoing chemotherapy depression incidence is 32.5%～75.7%, and the depressive emotion incidence rate of patients undergoing chemotherapy is 78.4% first.Simultaneously, cancer-related depressed (CRD) has following characteristics, and incidence rate is high, discrimination is low, it is low to treat, quality of life is low.CRD is having a strong impact on rehabilitation and the prognosis of tumor patient, and therefore studying the tumor complicated depression has important realistic meaning.
At present, the multiplex Western medicine of modern medicine carrys out Cure of depression, but the western medicine cycle is long, toxic and side effects is large, has greatly affected clinical efficacy.Therefore, removing to inquire into from the angle of Chinese medicine the antidepressant effect that develops Chinese medicine and prescription thereof has broad prospects.
Compound mylabris preparation is that to take Mylabris, Radix Ginseng, the Radix Astragali, Radix Et Caulis Acanthopanacis Senticosi, Rhizoma Sparganii, Herba Scutellariae Barbatae, Rhizoma Curcumae, Fructus Corni, Fructus Ligustri Lucidi, Fel Ursi powder, Radix Glycyrrhizae be primary raw material, extract the preparation that its extract with medical value is made as effective ingredient, having removing blood stasis becomes thin, the effect of counteracting toxic substances phagedenoma, wherein FUFANG BANMAO JIAONANG is recorded in the 17 of the Sanitation Ministry medicine standard Traditional Chinese medicine historical preparation, is mainly used in clinically primary hepatocarcinoma, pulmonary carcinoma, rectal cancer, malignant lymphoma, gynecologic malignant tumor etc.
The present patent application people is that ZL200710200660.4, name are called " compound cantharidin oral preparations and preparation method thereof " and the patent No. is, during ZL200710077864.3, name are called the patent of invention of " compound cantharis liquid formulation and preparation method thereof ", to disclose compound mylabris preparation prepared by ten a herbs such as Mylabris, Radix Ginseng, the Radix Astragali, Radix Et Caulis Acanthopanacis Senticosis and preparation method thereof in the patent No. respectively.Although research before shows compound mylabris preparation and can, for the preparation of the medicine of the various disease conditions such as treatment tumor, adjusting immunity, have no and utilize compound mylabris preparation to the patent documentation of the therapeutical effect of depression aspect and research report.The applicant is also unexpected discovery in research subsequently, and compound mylabris preparation can, for the treatment of depression disease, particularly have obvious therapeutic effect to tumor patient depression aspect.
Summary of the invention
The purpose of this invention is to provide a kind of cantharides Chinese medicine composition and the purposes of preparation in preparing the Cure of depression medicine thereof, especially the purposes in preparation treatment Tumor-assaciated depression of sex medicine.
The traditional Chinese medical science finds the research of depression, from the traditional Chinese medical science in theory, insufficiency of kidney-YANG and depression have certain contacting.Kidney yang is basis of all sun, the yang-energy of main the whole body, insufficiency of kidney-YANG, yang-energy loses in warm and agitates, cause essence and do not give birth to marrow, marrow void can not be filled brain, the brains inanition, gods are lost foster and are disease, the kidney being the origin of congenital constitution, and Syndrome Differentiation of Chinese Medicine is controlled depression, should pay attention to rousing oneself " initiatively " function of kidney yang, must improve better depressed all diseases by the kidney warming sun, thereby control depression for clinical distinguishing.
The prescription of compound mylabris preparation is: Mylabris, Radix Ginseng, the Radix Astragali, Radix Et Caulis Acanthopanacis Senticosi, Rhizoma Sparganii, Herba Scutellariae Barbatae, Rhizoma Curcumae, Fructus Corni, Fructus Ligustri Lucidi, Fel Ursi powder, Radix Glycyrrhizae.Radix Ginseng, have strongly invigorating primordial QI, invigorating the spleen to benefit the lung, and the beneficial blood that promotes the production of body fluid, calm the nerves and increase the effect of intelligence, for vigour collapse card, lung spleen heart syndrome of deficiency of kidney-QI, calentura deficiency of vital energy Tianjin wound, yearningly reaches diabete; The Radix Astragali, have the effect of invigorating QI to consolidate the body surface resistance, diuresis poison holding, evacuation of pus, expelling pus and promoting granulation,, anorexia and loose stool weak for the deficiency of vital energy, sinking of QI of middle-JIAO, exterior deficiency spontaneous perspiration; Radix Et Caulis Acanthopanacis Senticosi, have the effect of replenishing QI to invigorate the spleen, tonifying the kidney for tranquilization,, inappetence weak for deficiency of spleen-YANG and kidneyYANG, body void, soreness of waist and knee joint, insomnia and dreamful sleep.Radix Glycyrrhizae, have invigorating the spleen and benefiting QI, nourishing the lung to arrest cough, removing toxic substances and in, be in harmonious proportion the effect of hundred medicines, Fructus Ligustri Lucidi, belong to tonic, has the effect of liver and kidney tonifying, these several medical materials all belong to tonify deficiency QI invigorating class medicine from classification of TCD, and Synergistic, have good effect to kidney-yang nourishing.Simultaneously, the cantharidin in side in Mylabris can suppress DNA and RNA is synthetic, and liver and cancerous cell are had to stronger affinity.Effective to primary hepatic and other tumors, and without bone marrow inhibition, also there is the leukocyte increasing number, antiviral, antiinflammatory action.11 kinds of dialectical treating of medicine, the effect that there is warming and recuperating the kidney-YANG, invigorating the spleen and replenishing QI, heat-clearing and toxic substances removing, repercussive eliminating stagnation in anticancer.
Compound mylabris preparation of the present invention, according to listed as parts by weight, by raw material of Chinese medicine Mylabris 0.6-6 part, Radix Ginseng 1.5-15 part, Radix Astragali 7.5-100 part, Radix Et Caulis Acanthopanacis Senticosi 7.5-100 part, Rhizoma Sparganii 2.4-24 part, Herba Scutellariae Barbatae 9-100 part, Rhizoma Curcumae 2.4-24 part, Fructus Corni 3-30 part, Fructus Ligustri Lucidi 3-30 part, Fel Ursi powder 0.05-1.0 part, Radix Glycyrrhizae 1.5-15 part, through extraction, be prepared from.
Most preferred proportioning is: 3.97 parts of Mylabris, 9.92 parts of Radix Ginsengs, 49.58 parts of the Radixs Astragali, 49.58 parts of Radix Et Caulis Acanthopanacis Senticosis, 15.83 parts of Rhizoma Sparganii, 59.5 parts of Herba Scutellariae Barbataes, 15.83 parts of Rhizoma Curcumae, 19.83 parts of Fructus Corni, 19.83 parts of Fructus Ligustri Lucidi, 0.4 part of Fel Ursi powder, 9.92 parts, Radix Glycyrrhizae.
In when application, medicine of the present invention can be as required at least one conventional carrier pharmaceutically, as diluent, filler, binding agent, excipient, disintegrating agent, surfactant, absorption enhancer and lubricant etc.
Aforesaid compound mylabris preparation is preferably used with the form of capsule, injection, tablet, drop pill, granule, Emulsion, solution or suspending agent, more preferably capsule.Above-mentioned dosage form all can be according to the conventional method preparation of pharmaceutical field.
The route of administration of aforesaid compound mylabris preparation is oral, percutaneous, vein or intramuscular injection.
In the preparation method of compound mylabris preparation of the present invention, its effective component extracts can adopt the extraction process of FUFANG BANMAO JIAONANG in the 17 of the Sanitation Ministry medicine standard Traditional Chinese medicine historical preparation to extract, can be that the method that ZL200710200660.4, name are called " compound cantharidin oral preparations and preparation method thereof " is extracted according to the patent No., can be also that the method that ZL200710077864.3, name are called in " compound cantharis liquid formulation and preparation method thereof " is extracted according to the patent No., can also be extracted by conventional method.
The further experimental study discovery of the applicant, compound mylabris preparation really can be used for preparing the medicine of Cure of depression, especially for the medicine of preparation treatment tumor patient depression.
In order to make those of ordinary skills better understand the present invention, the applicant has carried out series of experiment research, to prove effect of the present invention:
One, antidepressant effect zoopery
This experiment adopts the autonomic activities experiment, forces the experiments such as outstanding tail and forced swimming to launch the mice behavior depression is observed, analyzed, by the antidepressant effect of a series of index explanation preparation of the present invention.
1.1 laboratory animal and material
Animal: Kunming mouse, the male and female dual-purpose, body weight 18-25g, the Guiyang Medical College Animal House provides.
Medicine: preparation of the present invention, the Chinese medicine extract made by Mylabris, Radix Ginseng, the Radix Astragali, Radix Et Caulis Acanthopanacis Senticosi, Rhizoma Sparganii, Herba Scutellariae Barbatae, Rhizoma Curcumae, Fructus Corni, Fructus Ligustri Lucidi, Fel Ursi powder, Radix Glycyrrhizae according to the preparation method of embodiment 1-3.
1.2 experimental technique
60 mices of experiment grouping are divided into cantharides experiment low dose group, cantharides experiment high dose group and Normal group at random, every group 20, with medicinal liquid, mice is carried out to gavage by body weight, dosage: preparation low dose group 10ml/kg of the present invention, preparation high dose group 30ml/kg of the present invention, Normal group does not award medicine, and normal the raising, carry out respectively following three kinds of experiments.
1.2.1 the continuous gavage of mice autonomic activities experiment mice 7 days, put into XZC-4A toy autonomic activities analyzer (Shandong Academy of Medical Sciences's manufacture) after last gavage 1h, after adapting to 3min, observe the movable number of times in 5min, and record result, in Table 1.
1.2.2 the continuous gavage of Tail suspension test mice 7 days is fixed in mice tail end 2cm place on horizontal plane after last gavage 1h, makes it be the reversal of the natural order of things state, the about 30cm of head destage face, surrounding, with plate isolation animal sight line, records the dead time of mice in 6min, in Table 2.
1.2.3 the preliminary experiment failure is rejected in the experiment of mice forced swimming, gavage is 7 days continuously, and after the last gavage 1h, mice is put into to diameter 22cm, the bucket of depth of water 10cm, 25 ℃ of water temperatures (with the preliminary experiment term harmonization), adapt to 2min, the dead time of mice in 4min after accumulative total, in Table 3.
1. all experimental results of 4 statistical procedures all mean with x ± s, adopt the SPSS statistical software to carry out one factor analysis of variance and rank test.
2. result
2. the impact of 1 preparation of the present invention on the mice autonomic activities
Two dosage groups of preparation of the present invention are compared equal not statistically significant with Normal group, the results are shown in Table 1.
The impact (x ± s) of table 1 preparation of the present invention on the mice autonomic activities
| Group | Dosage | Animal (only) | The 5min autonomic activities time (s) |
| Normal group | ---- | 20 | 144.56±26.79 |
| Low dose group | 10ml/kg | 20 | 130.89±38.19 |
| High dose group | 30ml/kg | 20 | 157.67±32.51 |
2. the impact of 2 preparations of the present invention on the Tail suspension test dead time
Preparation low dose group of the present invention, high dose group can obviously shorten the outstanding tail experiment small mouse dead time (P<0.05 or 0.01), and the high dose group better effects if, the results are shown in Table 2.
The impact (x ± s) of table 2 preparation of the present invention on the Tail suspension test dead time
| Group | Dosage | Animal (only) | The 6min autonomic activities time (s) |
| Normal group | ---- | 20 | 116.11±45.73 |
| Low dose group | 10ml/kg | 20 | 81.33±45.10● |
| High dose group | 30ml/kg | 20 | 63.93±48.22●● |
With Normal group, compare, ● P<0. 05, ● ● P<0. 01, following table is same.
2. 3 preparations of the present invention are on the impact of experiment dead time of mice forced swimming
Preparation low dose group of the present invention, high dose group can obviously shorten the outstanding tail experiment small mouse dead time, and the high dose group better effects if, the results are shown in Table 3.
Table 3 preparation of the present invention is on the impact (x ± s) of experiment dead time of mice forced swimming
| Group | Dosage | Animal (only) | The 4min autonomic activities time (s) |
| Normal group | ---- | 20 | 82.33±63.24 |
| Low dose group | 10ml/kg | 20 | 37.25±45.42 |
| High dose group | 30ml/kg | 20 | 35.45±51.40 |
Tail suspension test and forced swimming experiment are for screening and observe the reliable experimental technique of antidepressant drug in the Behavioral pharmacology experiment.Above experimental data shows, with the Normal group experiment, compare, preparation of the present invention is low, high dose all can obviously shorten outstanding tail and the dead time of forced swimming experiment small mouse, and its autonomic activities is not made significant difference, show that preparation of the present invention has antidepressant effect preferably, and without the central excitation effect.
Two, the effect of the present invention to the tumor patient depression
1, physical data
120 routine tumor patients, main manifestations is depressed, without positive mental disorder and family history, mind is clear, without understanding dysfunction.100 routine patients are divided into two groups at random.Treatment group 60 examples, male 31 examples, female's 29 examples, 48 ± 16 years old age; Matched group 60 examples, male 30 examples, female's 30 examples, 45 ± 15 years old age.
The diagnosis of depression, evaluated by the Psycs.To 120 routine respondents, after being in hospital 1 week, according to the evaluation of Hamilton depression scale (24 HANMD) methods of marking, methods of marking is: every minute 5 kinds of degree in 24 projects, nothing is light, in, heavy, very heavy, score value is respectively 0,1,2,3,4 minutes, every score was added up.The HANMD total points is more than or equal to 35 and is divided into major depression, and 20-35 are divided into the moderate depression, and 8-20 are divided into slight depression, and the factor is divided into 7 factor structures and concludes.Treatment group major depression 9 examples, account for 18%, and light to moderate depressed 41 examples, account for 82%; Matched group severe depression 6 examples, account for 12%, and light to moderate depressed 44 examples, account for 88%.
2, Therapeutic Method
Treatment group: treatment group is divided into to 6 groups at random, every group of 10 people.Every group gives the pharmaceutical preparation that the embodiment of the present invention makes, and administration kind, administering mode and dosage are as follows: the preparation of the present invention that embodiment 4 makes each 3 oral for group 1, every day 2 times; The each 100ml of preparation of the present invention that embodiment 5 makes with 5% glucose 450ml dilution after for group 2, intravenous drip, once a day; The preparation of the present invention that embodiment 6 makes each 3 oral for group 3, every day 2 times; Each 3 bag mouth of the preparation of the present invention that embodiment 7 makes are taken in group 4, every day 2 times; Each 3 bag mouth of the preparation of the present invention that embodiment 8 makes are taken in group 5, every day 2 times; The preparation of the present invention that embodiment 9 makes each 2 with after 5% glucose 450ml dilution for group 6, intravenous drip, once a day; It within 14 days, is 1 course for the treatment of.Matched group only carries out the psychotherapy.
3, criterion of therapeutical effect and therapeutic outcome
Adopt Hamilton depression scale (24 HANMD) evaluation curative effect.Cure in the recent period O～8 minute, produce effects 9～20 minutes, take a turn for the better 21～35 minutes, invalid more than 35 minutes.The results are shown in Table 4, table 5.
Each group factor score value of table 4 is (x ± s) unit relatively: minute
Before treatment, two groups of every factor score values are compared equal not statistically significant, have comparability.
Table 5 liang group symptom curative effect comparative unit: example
| n | Cure in the recent period | Produce effects | Take a turn for the better | Invalid | Total effective rate | |
| Treatment group | 60 | 20 | 30 | 4 | 6 | 90.0（%） |
| Matched group | 60 | 12 | 18 | 19 | 11 | 81.0（%） |
Each group factor score value of table 4 data declaration relatively, compare with matched group, adopts preparation of the present invention after the every factor score value reduction is in various degree all arranged, depressive symptom alleviates, and point out preparation of the present invention to have and is better than the effect that psychotherapy's cancer-related depression is demonstrate,proved.
Table 5 liang group symptom curative effect comparing data explanation, the total effective rate for the treatment of group is higher than matched group, and total effective rate is 90.0%, its healing near-mid term, produce effects and totally 54 examples that take a turn for the better, inefficiency accounts for 10%.And matched group, inefficiency accounts for 19%, and prompting is compared with the psychotherapy, the better efficacy of the depressed card of preparation for treating psychotherapy cancer-related of the present invention.
The specific embodiment:
Below, enumerate embodiment the present invention is further described, but the present invention is not limited to following embodiment.
Embodiment 1:
(1) take according to the following ratio raw material:
Mylabris 3.97g, Radix Ginseng 9.92g, the Radix Astragali 49.58 g, Radix Et Caulis Acanthopanacis Senticosi 49.58g, Rhizoma Sparganii 15.83g, Herba Scutellariae Barbatae 59.5g, Rhizoma Curcumae 15.83g, Fructus Corni 19.83g, Fructus Ligustri Lucidi 19.83g, Fel Ursi powder 0.4g, Radix Glycyrrhizae 9.92g
(2) preparation method:
Above ten simply, and except Fel Ursi powder, Radix Ginseng, Fructus Corni, Fructus Ligustri Lucidi, Herba Scutellariae Barbatae are ground into fine powder, sieve, standby; Mylabris is soaked and extracts 3 times with chloroform, and each 95.2ml, soak 72 hours, and merge extractive liquid, reclaims chloroform, is concentrated into the thick paste shape; The five tastes such as all the other Radixs Astragali decoct with water three times, and 3 hours for the first time, the 2nd time 1.5 hours, 1 hour for the third time, collecting decoction, filtered, and filtrate is concentrated into relative density and is about 1.20 (80 ℃); Fel Ursi powder adds the fine powders such as above-mentioned thick paste, concentrated solution and Radix Ginseng after adding 80 ℃ of water dissolutioies, fully stirs evenly, and makes Chinese medicine extract.
Embodiment 2:
(1) take according to the following ratio raw material:
Mylabris 0.6g, Radix Ginseng 1.5g, the Radix Astragali 7.5 g, Radix Et Caulis Acanthopanacis Senticosi 7.5g, Rhizoma Sparganii 2.4g, Herba Scutellariae Barbatae 9.0g, Rhizoma Curcumae 2.4g, Fructus Corni 3g, Fructus Ligustri Lucidi 3g, Fel Ursi powder 0.05g, Radix Glycyrrhizae 1.5g
(2) preparation method:
Simply, except Fel Ursi powder, it is 60 purpose fine powders that Mylabris is pulverized to Fang Zhongshi, water extraction 4 times, add the water of 22 times of amounts for the first time, soaks 30 minutes, extract 2 hours, add for the second time the water of 15 times of amounts, extract 1 hour, the water that adds for the third time 12 times of amounts, extract 1 hour, add the water of 10 times of amounts the 4th time, extract 1 hour, filter merging filtrate, the 9 flavor water extraction such as all the other Radix Ginsengs 3 times, add for the first time 15 times of water gagings, soak 1 hour, extract 4 hours, add for the second time 10 times of water gagings, extract 1.5 hours, add for the third time 8 times of water gagings, extract 1.5 hours, filter, filtrate merges, being concentrated into relative density is 1.15 60 ℃ of surveys, let cool, adding ethanol is 60% to measuring containing alcohol, cold preservation 24 hours, filter, filtrate recycling ethanol, continue to be concentrated into that relative density surveys in the time of 60 ℃ is 1.25 thick paste, add water to 70% of total amount, at 8 ℃ of cold preservation 48h, get supernatant, add Fel Ursi powder to make to dissolve, adding the Mylabris extracting solution mixes, make Chinese medicine extract.
Embodiment 3:
(1) take according to the following ratio raw material:
Mylabris 6.0g, Radix Ginseng 15g, the Radix Astragali 100 g, Radix Et Caulis Acanthopanacis Senticosi 100g, Rhizoma Sparganii 24g, Herba Scutellariae Barbatae 100g, Rhizoma Curcumae 24g, Fructus Corni 30g, Fructus Ligustri Lucidi 30g, Fel Ursi powder 1.0g, Radix Glycyrrhizae 15g
(2) preparation method:
Preparation method is: simply, except Fel Ursi powder, powder of cantharide is broken into 60 purpose coarse powder to Fang Zhongshi, water extraction 3 times, add the water of 20 times of amounts for the first time, soaks 40 minutes, extract 1.5 hours, second and third time adds the water of 15 times of amounts at every turn, extracts 1.0 hours, filter, merging filtrate, add ethanol to containing the alcohol amount, reaching 80%, standing, filter, filtrate decompression is condensed into thick paste, adds the water of 8 times of amounts, stir, standing, filter filtrate centrifugal 20 minutes with the speed of 10000 rev/mins, the centrifugal liquid concentrating under reduced pressure, dry, pulverize, obtain Mylabris extract;
Get the 9 flavor medical materials such as all the other Radix Ginsengs, pulverize, extract 3 times merge extractive liquid, with the water of 10 times of amounts by decocting method, infusion process, filter, filtrate decompression is condensed into relative density at 60 ℃ of medicinal liquids that are 1.10, adds ethanol to being 60% containing the alcohol amount, standing 24 hours, filter, must precipitate and supernatant.The supernatant concentrating under reduced pressure becomes thick paste, adds the water of 10 times of amounts, stirs standing, filter, processed good polyamide column on filtrate, first wash with water to colourless, use again 50% ethanol elution of 8 times of amounts instead, collect ethanol elution, decompression recycling ethanol, on concentrated solution, processed good macroporous adsorptive resins, first wash with water to colourless, then use 60% ethanol elution instead, collect ethanol elution, concentrating under reduced pressure, drying, obtain total saponin extracts;
Get precipitation decompression cold drying, add appropriate distilled water and dissolve, add ethanol to containing the alcohol amount, reaching 80%, standing 24 hours, filter, the precipitation adding distil water is dissolved into 1% solution, crosses hollow fiber column ultrafilter (molecular cut off is greater than 6000), and ultrafiltrate is evaporated to dry; Perhaps get after the secondary precipitate with ethanol gained precipitation and add again appropriate distilled water and dissolve, add ethanol to containing the alcohol amount, reaching 75%, standing 24 hours, filter, get precipitation with appropriate absolute ethanol washing 1-2 time, obtain the polysaccharide active component;
Above-mentioned Mylabris extract, total saponin extracts, polysaccharide active component are fully mixed, dissolve with water for injection, then add Fel Ursi powder to make to dissolve, make Chinese medicine extract.
Embodiment 4:
Get the Chinese medicine extract that in embodiment 1-3, any one method makes, drying below 80 ℃, be ground into fine powder, incapsulate, make the 6O00 grain, obtain the FUFANG BANMAO JIAONANG agent, every dress 0.25g.
Embodiment 5:
Get the Chinese medicine extract that in embodiment 1-3, any one method makes, inject water 100ml embedding in corresponding container, hot pressing (115 ℃) sterilizing secondary, each 30 minutes, after cooling, the paper pulp sucking filtration, filtrate is diluted to 1000ml with water for injection, by the incipient fusion filter bulb, filters, embedding, 115 ℃ of sterilizings 30 minutes again, obtain banmao complex injection, specification: 10ml/ props up.
Embodiment 6:
Get the Chinese medicine extract that in embodiment 1-3, any one method makes, 40 ℃ are dried to fully, fully mix, and granulate, with 16 mesh sieve granulate, adding 1% magnesium stearate is lubricant, adds microcrystalline Cellulose, and amylum pregelatinisatum fully mixes, be pressed into 6000, film coating, obtain the cantharides tablet, every 0.25g on chip.
Embodiment 7:
Get the Chinese medicine extract that in embodiment 1-3, any one method makes, 40 ℃ are dried to fully, fully mix, add in the Polyethylene Glycol M6000 of molten condition, stir, it is uniformly dispersed, be incubated standby, by the melting substrate of mix homogeneously, as in the attemperator pill dripping machine and keep substrate in stirring, start is instiled, and makes the coolant of drop pill molding of liquid paraffin or silicone oil.The drop pill of making is taken out from coolant, carry out Rapid Cleaning with a small amount of dehydrated alcohol and be placed on forced air drying under 30 ~ 40 ℃ of conditions in the dish of stand, choose ball, glazing, packing, obtain the compound cantharis drop pill agent, specification: the 0.25g/ bag.
Embodiment 8:
Get the Chinese medicine extract that in embodiment 1-3, any one method makes, 40 ℃ are dried to fully, fully mix, then to add dextrin to total amount are 1000g, pulverize, mix, granulate, drying, granulate, packing, every packed 0.25g, obtain the cantharides granule, every packed 0.25g.
Embodiment 9:
The Chinese medicine extract that in embodiment 1-3, any one method makes, be dissolved in dehydrated alcohol, adds Cremophor EL, and mix and blend dissolves; After being dissolved with water for injection, mannitol adds again in above-mentioned Chinese medicine extract alcoholic solution, stirring and evenly mixing, with the dilute hydrochloric acid adjust pH, be 3.54, add water for injection to be stirred to Chinese medicine extract and be dissolved to the solution clarification, the active carbon that adds supplementary material total amount 0.1%, stirring at room 20 minutes, by 0.8 μ m filter membrane coarse filtration, filtrate injects water to 6000ml(routine prescription amount), use again 0.22 μ m filter membrane fine straining, be sub-packed in cillin bottle, put into freeze dryer and carry out lyophilizing, obtain 1000 of injection use compound Mylabris lyophilized preparation, specification: 30mg/ props up.
Claims (7)
1. the purposes of a cantharides Chinese medicine composition in preparing the Cure of depression medicine, it is characterized in that: described compositions, according to listed as parts by weight, by Mylabris 0.6-6 part, Radix Ginseng 1.5-15 part, Radix Astragali 7.5-100 part, Radix Et Caulis Acanthopanacis Senticosi 7.5-100 part, Rhizoma Sparganii 2.4-24 part, Herba Scutellariae Barbatae 9-100 part, Rhizoma Curcumae 2.4-24 part, Fructus Corni 3-30 part, Fructus Ligustri Lucidi 3-30 part, Fel Ursi powder 0.05-1.0 part, Radix Glycyrrhizae 1.5-15 part, through extraction, be prepared from.
2. cantharides Chinese medicine composition according to claim 1 is in the purposes in the Cure of depression medicine, it is characterized in that: described compositions, according to listed as parts by weight, by 9.92 parts of 3.97 parts of Mylabris, 9.92 parts of Radix Ginsengs, 49.58 parts of the Radixs Astragali, 49.58 parts of Radix Et Caulis Acanthopanacis Senticosis, 15.83 parts of Rhizoma Sparganii, 59.5 parts of Herba Scutellariae Barbataes, 15.83 parts of Rhizoma Curcumae, 19.83 parts of Fructus Corni, 19.83 parts of Fructus Ligustri Lucidi, 0.4 part of Fel Ursi powder, Radix Glycyrrhizae, through extraction, be prepared from.
3. the purposes of a compound mylabris preparation in preparing the Cure of depression medicine, it is characterized in that: this compound mylabris preparation by the described cantharides Chinese medicine composition of claim 1 add at least one pharmaceutically conventional carrier adopt conventional method to be prepared from, carrier is conventional as diluent, filler, binding agent, excipient, disintegrating agent, surfactant, absorption enhancer and lubricant.
4. the purposes of compound mylabris preparation in preparing the Cure of depression medicine according to claim 3, is characterized in that, this compound mylabris preparation is capsule, injection, tablet, drop pill, granule, Emulsion, solution, suspending agent.
5. according to the purposes of the described compound mylabris preparation of claim 3 in preparing the Cure of depression medicine, it is characterized in that: the route of administration of this compound mylabris preparation is oral, percutaneous, vein or intramuscular injection.
6. the purposes of compound mylabris preparation in preparing the Cure of depression medicine according to claim 4, is characterized in that, this compound mylabris preparation is capsule.
7. according to the purposes of the arbitrary described compound mylabris preparation of claim 4-6 in preparing the Cure of depression medicine, it is characterized in that the purposes of described compound mylabris preparation in preparation treatment Tumor-assaciated depression of sex medicine.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105769876A (en) * | 2016-03-08 | 2016-07-20 | 上海中医药大学 | Medical application of bile acid ingredient and bear bile |
| CN105920447A (en) * | 2016-06-12 | 2016-09-07 | 贵州神奇药物研究院 | Antidepressant health product and preparation method thereof |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN101091784A (en) * | 2007-05-21 | 2007-12-26 | 贵州益佰制药股份有限公司 | Compound oral taking preparation of cantharis, and preparation method |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN101091784A (en) * | 2007-05-21 | 2007-12-26 | 贵州益佰制药股份有限公司 | Compound oral taking preparation of cantharis, and preparation method |
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| 田杰等: "艾迪注射液改善肿瘤患者生活质量的研究进展", 《中国肿瘤临床与康复》 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105769876A (en) * | 2016-03-08 | 2016-07-20 | 上海中医药大学 | Medical application of bile acid ingredient and bear bile |
| CN105769876B (en) * | 2016-03-08 | 2019-01-29 | 上海中医药大学 | The medical usage of Bile acid and bear gall |
| CN105920447A (en) * | 2016-06-12 | 2016-09-07 | 贵州神奇药物研究院 | Antidepressant health product and preparation method thereof |
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| CN103417906B (en) | 2015-09-09 |
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