CN103210840A - Anthocyanin radish breeding method - Google Patents
Anthocyanin radish breeding method Download PDFInfo
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- CN103210840A CN103210840A CN2013101805198A CN201310180519A CN103210840A CN 103210840 A CN103210840 A CN 103210840A CN 2013101805198 A CN2013101805198 A CN 2013101805198A CN 201310180519 A CN201310180519 A CN 201310180519A CN 103210840 A CN103210840 A CN 103210840A
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Abstract
The invention discloses an anthocyanin radish breeding method. The method comprises the following steps: (1) selecting a female parent and a male parent to hybridize; (2) continuously utilizing a sterile plant in a filial generation as the female parent to continuously back cross with the original male parent; (3) after back crossing for 3-4 generations, finding a sensitive sterile plant with a micro amount of pollen in a flowering period to carry out bud stage selfing; (4) taking a male sterile plant as the female parent to test cross with the original male parent; and (5) selecting a system with stable sterility, anthocyanin content being more than 160mg/100g fresh weight, thioglycodise content being less than 95mg/100g fresh weight, excellent comprehensive characters and good combining capability as a dominant male sterile line, and obtaining a back cross male parent which is a maintainer line. The anthocyanin radish bred by adopting the method has the anthocyanin content being more than 167mg/100g fresh weight and the thioglycodise content being less than 90mg/100g fresh weight; and the anthocyanin radish has the advantages of difficult hollowness, difficult inner flowering, disease resistance, bolting resistance and stable performance, so that anthocyanin can be conveniently extracted and processed.
Description
Technical field
The present invention relates to a kind of anthocyan radish, especially a kind of breeding method of utilizing dominance male sterile line preparation anthocyan radish belongs to field of plant variety breeding technology.
Background technology
The anthocyan radish is cheap, source abundance and pigment are stable, has the market competitiveness.The anthocyan international market price of separate sources in 2012 reaches 700～1500 dollars/kilogram.The main grown place of anthocyan in South America, ground such as America and Europe, Asia, South America mainly produces the anthocyan with Qarnet rice source, Europe is raw material with fruit classes such as grape, elder berry, blueberry, gooseberry, wild cherries, its price comparison costliness.China is the main source of anthocyan with raw materials such as radish, grapes, and radish anthocyan is because the stability of its excellence is subjected to very big attention.Mainly utilize self incompatible line seed selection anthocyan radish on producing at present, since the self incompatible line preparation anthocyan radish first generation of hybrid, its parent propagation cost height, and the seed hybrid rate is difficult to reach 100%.In addition, the mixing and degradation phenomenas such as a kind of sedge, the chaff heart early of existing anthocyan radish colony ubiquity leaf look, blade profile, root shape, output is generally not high, causes the not high and sulphur glycosides too high levels of anthocyanidin content.
Summary of the invention
The purpose of this invention is to provide that a kind of precocity, high yield, high-quality, anthocyanidin content are higher than the 160mg/100g fresh weight, sulphur glycosides content is lower than the 95mg/100g fresh weight, disease resistance is strong, is fit to the breeding method of the anthocyan radish of anthocyan processing.
The present invention is achieved by the following technical programs:
The breeding method of a kind of anthocyan radish may further comprise the steps:
(1) in the segregation population of anthocyan radish dominance male sterile starting material, it is maternal selecting fresh weight, comprehensive proterties sterile strain good, that sterility is responsive, and selecting the self-compatible that comprehensive proterties is good, stable, coordinate force is good is that male parent is hybridized;
(2) continuing with sterile strain in filial generation is maternal and former male parent continuous backcross;
(3) after 3～4 generations of backcrossing, enlarge backcross progeny colony plant quantity, and searching there is the sensitive sterile strain of trace-pollen to do the selfing of flower bud phase in flowering stage;
(4) be maternal with the stable male sterile plant of the sterility that occurs among the sensitive strain self progeny, with former male parent test cross;
(5) identify these test crosses offspring's male sterile sex expression in the field, sterile plant rate reaches 100% the dominance male sterile plant that isozygotys that is; Test cross offspring to the dominance male sterile plant that isozygotys identifies, selects the dominance male sterile line that sterility is stable, anthocyanidin content is higher than the 160mg/100g fresh weight, sulphur glycosides content is lower than the 95mg/100g fresh weight, the comprehensive system that proterties is good, neat, coordinate force is good makes up as preparing hybrid; The male parent of backcrossing is maintenance line.
Purpose of the present invention can also further realize by following technical measures.
The breeding method of aforesaid anthocyan radish, wherein female parent of selecting in the step 1) and male parent anthocyanidin content all are higher than the 160mg/100g fresh weight, and sulphur glycosides content all is lower than the 95mg/100g fresh weight.
The anthocyan radish of adopting the present invention to cultivate is moderate relatively picking time, and skin aubergine, yellowish pink redness, dense structure, matter are tender crisp.Plant height 58～61cm, branches and leaves degree of development 59～63cm, leafage half is upright, and it is green that half flower leaf type, 1～2 pair of the shallow side decomposite leaf that splits, 14～15 of siphonal lobes, blade oval are, stem, petiole and middle rib aubergine, the lateral vein green, sowing is to gathering in the crops about 93 days.The anthocyan radish is the big slightly oval tubular in bottom, about single heavy 192g; The anthocyan radish yield is brought up to 2550kg/667m
2, the anthocyanidin content of anthocyan radish is higher than the 167mg/100g fresh weight, and sulphur glycosides content is lower than the 90mg/100g fresh weight.Be difficult for the chaff heart, to be difficult for bolting fickle in love, antiviral, anti-and proterties stable, helps extracting processing anthocyan.
Advantage of the present invention and characteristics will make an explanation by the non-limitative illustration of following preferred embodiment, and these embodiment only provide as an example.
Embodiment
The invention will be further described below in conjunction with the embodiment of Jurong City, Jiangsu Province.
The present invention includes following steps:
In late March, (1) first, in anthocyan radish dominance male sterile starting material segregation population, select anthocyanidin content to be higher than the 160mg/100g fresh weight, sulphur glycosides content is lower than 95mg/100g fresh weight, the comprehensive sterile strain 02-1 that proterties is good, sterility is responsive for maternal, is higher than with anthocyanidin content that 160mg/100g fresh weight, sulphur glycosides content are lower than the 95mg/100g fresh weight, the comprehensive self compatible line 03-6 that proterties is good, stable, coordinate force is good is that male parent is hybridized.
(2) second to the 4th years continue with sterile strain in filial generation is maternal and former male parent 03-6 continuous backcross.
After 3～4 generations of backcrossing, enlarged backcross progeny colony plant quantity and reach 120 strains in (3) the 5th years, and seek 8 of the sensitive sterile strains that trace-pollen is arranged, and do the selfing of flower bud phase in flowering stage.
(4) the 6th year spring was maternal with the stable male sterile plant of the sterility that occurs among the sensitive strain self progeny, with former male parent 03-6 test cross.
(5) the 6th year autumn identified these test crosses offspring's anthocyanidin content, sulphur glycosides content, comprehensive proterties in the field; The 7th year spring observed test cross offspring male sterile sex expression, and test cross offspring sterile plant rate reaches 100%, and then the maternal sterile strain of this test cross combination is the dominance male sterile plant that isozygotys.Test cross offspring to the dominance male sterile plant that isozygotys identifies, select the dominance male sterile line that sterility is stable, anthocyanidin content is higher than the 160mg/100g fresh weight, sulphur glycosides content is lower than the 95mg/100g fresh weight, the comprehensive system that proterties is good, neat, coordinate force is good makes up as preparing hybrid, the male parent of backcrossing is maintenance line.With above-mentioned continuous backcross transfer method, successively with 03-6,02-4,03-12,04-3,04-15 totally 5 dissimilar anthocyan inbred lines carry out the transformation of dominance male sterile line as male parent, annual identify backcross, about 40 parts of test cross combinations.By steps such as continuous backcross, selfing and test crosses, successively doing with dissimilar inbred lines in the backcross progeny of male parent, identify 9 of the dominance male sterile plants of isozygotying.The sterility of the dominance male sterile line that sterile strain is prepared to the dominance of isozygotying with these, anthocyanidin content, sulphur glycosides content, coordinate force etc. are investigated comprehensively, therefrom filter out the 03-6 MsMs(dominance sterile gene type that isozygotys) but the good dominance male sterile line of practical application, reach more than 100% or 99% at each annual sterile degree, the proterties of blossoming and bearing fruit is normal, anthocyanidin content is higher than the 160mg/100g fresh weight, sulphur glycosides content is lower than 95 mg/100g fresh weights, the comprehensive good and neat and consistent of proterties, coordinate force is good, can be used for preparing the hybrid combination of dissimilar anthocyan radish.
The 7th year spring, with anthocyan radish dominance caryogram male sterile line 03-6 MsMs is female parent, is higher than with anthocyanidin content that 160mg/100g fresh weight, sulphur glycosides content are lower than the 95mg/100g fresh weight, the good radish inbred line 0727 through 5 generations selfing purifying of coordinate force is male parent preparation anthocyan radish new varieties " U.S. red ".
Following table is anthocyan radish new varieties " U.S. red " and the quality contrast of contrast groups (CK) existing Fuling carrot (CK1), northern Suzhou carrot (CK2):
| ? | Anthocyanidin content (mg/100g fresh weight) | Sulphur glycosides content (mg/100g fresh weight) | The pH value | Moisture (%, w/w) | Sugar content (mg/100g fresh weight) |
| U.S. red | 167.21±9.3 | 88.72±1.76 | 5.72 | 91.34 | 152.13±15.45 |
| Fuling carrot (CK1) | 160.74±8.81 | 90.17±2.48 | 5.67 | 91.46 | 131.15±16.54 |
| Northern Suzhou carrot (CK2) | 143.56±4.71 | 91.67±1.8 | 5.64 | 91.43 | 167.56±16.71 |
Can find out from table: the anthocyanidin content by the anthocyan radish new varieties of breeding of the present invention " U.S. is red " is higher than contrast groups, and sulphur glycosides content is lower than contrast groups.
In addition to the implementation, the present invention can also have other embodiments, and all employings are equal to the technical scheme of replacement or equivalent deformation one-tenth, all drop in the protection domain of requirement of the present invention.
Claims (2)
1. the breeding method of an anthocyan radish is characterized in that: may further comprise the steps:
(1) in the segregation population of anthocyan radish dominance male sterile starting material, it is maternal selecting fresh weight, comprehensive proterties sterile strain good, that sterility is responsive, and selecting the self-compatible that comprehensive proterties is good, stable, coordinate force is good is that male parent is hybridized;
(2) continuing with sterile strain in filial generation is maternal and former male parent continuous backcross;
(3) after 3～4 generations of backcrossing, enlarge backcross progeny colony plant quantity, and searching there is the sensitive sterile strain of trace-pollen to do the selfing of flower bud phase in flowering stage;
(4) be maternal with the stable male sterile plant of the sterility that occurs among the sensitive strain self progeny, with former male parent test cross;
(5) identify these test crosses offspring's male sterile sex expression in the field, sterile plant rate reaches 100% the dominance male sterile plant that isozygotys that is; Test cross offspring to the dominance male sterile plant that isozygotys identifies, selects the dominance male sterile line that sterility is stable, anthocyanidin content is higher than the 160mg/100g fresh weight, sulphur glycosides content is lower than the 95mg/100g fresh weight, the comprehensive system that proterties is good, neat, coordinate force is good makes up as preparing hybrid; The male parent of backcrossing is maintenance line.
2. the breeding method of anthocyan radish as claimed in claim 1 is characterized in that, female parent of selecting in the step 1) and male parent anthocyanidin content all are higher than the 160mg/100g fresh weight, and sulphur glycosides content all is lower than the 95mg/100g fresh weight.
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104160945A (en) * | 2014-06-06 | 2014-11-26 | 镇江瑞繁农艺有限公司 | Method for breeding novel species by hybridization of broad-leaf radish and petal-leaf radish |
| CN104160948A (en) * | 2014-08-12 | 2014-11-26 | 镇江瑞繁农艺有限公司 | Breeding method for rapidly obtaining cytoplasm male sterility line and maintenance line of carmine radish |
| CN104303995A (en) * | 2014-09-02 | 2015-01-28 | 镇江瑞繁农艺有限公司 | Breeding method of carmine radish |
| CN104719132A (en) * | 2015-02-04 | 2015-06-24 | 江苏沿海地区农业科学研究所 | Seed selection method of springtime bolting-resistant long cylindrical green-shoulder and white-skin radish |
| CN104938326A (en) * | 2015-07-06 | 2015-09-30 | 山东省农业科学院作物研究所 | Rapid seed selection method of high-anthocyanin purple sweet potato variety |
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104160945A (en) * | 2014-06-06 | 2014-11-26 | 镇江瑞繁农艺有限公司 | Method for breeding novel species by hybridization of broad-leaf radish and petal-leaf radish |
| CN104160948A (en) * | 2014-08-12 | 2014-11-26 | 镇江瑞繁农艺有限公司 | Breeding method for rapidly obtaining cytoplasm male sterility line and maintenance line of carmine radish |
| CN104303995A (en) * | 2014-09-02 | 2015-01-28 | 镇江瑞繁农艺有限公司 | Breeding method of carmine radish |
| CN104303995B (en) * | 2014-09-02 | 2016-08-24 | 镇江瑞繁农艺有限公司 | A kind of breeding method of rouge radish |
| CN104719132A (en) * | 2015-02-04 | 2015-06-24 | 江苏沿海地区农业科学研究所 | Seed selection method of springtime bolting-resistant long cylindrical green-shoulder and white-skin radish |
| CN104719132B (en) * | 2015-02-04 | 2017-11-21 | 江苏沿海地区农业科学研究所 | A kind of selection of the white skin radish of the oval tubular green grass or young crops shoulder of spring resistance to bolting |
| CN104938326A (en) * | 2015-07-06 | 2015-09-30 | 山东省农业科学院作物研究所 | Rapid seed selection method of high-anthocyanin purple sweet potato variety |
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Application publication date: 20130724 |