CN104160948A - Breeding method for rapidly obtaining cytoplasm male sterility line and maintenance line of carmine radish - Google Patents
Breeding method for rapidly obtaining cytoplasm male sterility line and maintenance line of carmine radish Download PDFInfo
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Abstract
The invention discloses a breeding method for rapidly obtaining a cytoplasm male sterility line and a maintenance line of carmine radish. The method mainly comprises the following steps: selecting different types of carmine radish materials, performing first generation selfing, determining a selective breeding object, performing orientation selection on a single plant, and performing second generation selfing; performing orientation selection on the single plant for continuously selfing in a selfing offspring group, and performing orientation selection on the single plant according to the selective breeding object; selecting cytoplasm male sterility as a female parent, selecting the selected single plant as a male parent, performing interspecies cross, obtaining hybrid F1, thereby transferring CMS genes of the sterility to collard; continuously selfing and purifying the selfing offspring, and screening a sterile plant which is the closest to the selective breeding object of the male parent to serve as a female parent in a cytoplasm male sterility hybrid plant group, taking the screen single plant which is the closest to the selective breeding object in the selfing offspring as a male parent for continuously performing paired cross; and purifying the selfing offspring, transferring the male sterility, simultaneously performing paired cross on each generation, and selfing multiple generations to obtain the maintenance line and the male sterility line. According to the method, the labor and cost can be obviously reduced, and the breeding time is shortened.
Description
Technical field
The present invention relates to a kind of breeding method of rouge radish, obtain especially fast the breeding method of rouge radish cytoplasm male sterility line and maintainer, belong to field of plant variety breeding technology.
Background technology
Rouge radish (Raphanus Sativus L.Sinoruber Makino) claim again turnip with red inside, belong to landrace under Cruciferae Rhaphanus radish kind, complete red famous with carpel, be rich in anthocyan, Fuling District, main product Chongqing City, also there is cultivation in the provinces such as contiguous cities and counties and Sichuan, Guizhou.In recent years, a lot of units all taking rouge radish fleshy root as raw material extracts natural anthocyan.Radish red pigment is a kind of natural food colour, and safe, nontoxic, aboundresources, and have certain nutrition and pharmacological action are that national additive standard committee allows one of natural colouring matter using.It has that chemical stability is good, storage-stable, the excellent feature of warm tolerance, and in addition, it also has certain non-oxidizability, is the colouring agent that human body is had to health care.
Obviously, the aspects such as output, quality and resistance all show hybrid vigour in Heterosis In Radish performance.Research shows, utilize cytoplasm male sterility line preparing hybrid kind, have that method is easy, genetic stability, hybrid rate are high, time saving and energy saving, sterility advantage thoroughly, and can effectively control the outflow of merit gene, aspect rouge radish crossbreeding, have significant application value.And male sterile with radish cytoplasm (CMS) is a kind of natural phenomena, show as maternal inheritance, pollen abortion and gynoecium normal, use the pollen of fertile plant to sterile strain gynoecium pollination, can be normally solid.Rouge radish is mainly gathered in the crops trophosome-radish fleshy root, but not obtains seed, so do not need restorer in the time of hybrid seeding, therefore obtaining rouge radish cytoplasm male sterility line and maintainer is to utilize the key of male sterile preparing hybrid kind.
At present, also there are a lot of problems in the rouge radish kind using in production, and main manifestations is that germ plasm resource lacks; Existing resource proterties is uneven, and leaf look, blade profile, root type differ in a jumble; False red heart, color of the leather is neat, and yellowish pink, taking fickle in love as main, generally mixes the white heart, and partial mass is without typical red heart, and the complete red characteristic of carpel is not remarkable; Pigment content is low, and fresh output is not high.Therefore, need extensive collection badly, arrange domestic and international rouge radish germ plasm resource, utilize GENERALIZATION OF MODERN BREEDING TECHNIQUE means to purificate and rejuvenate, select and there is the full rouge radish inbred line breeding material red remarkable characteristic, dissimilar of carpel, and preparing hybrid kind, improve the level of resources utilization.Less about the research of rouge radish cytoplasm male sterility line and maintainer backcross transformation and directive breeding, utilize male sterile line to prepare the report of rouge radish crossbreed but have.(the China's Vegetable such as Lv Fasheng, 2012 (2): 104-106) and (China's Vegetable such as Tao Hongying, 2012 (1): 37) utilize respectively the male sterile line of seed selection to make No. 1, kermes and No. 2 turnip with red inside crossbreed of kermes, but do not elaborate for the Breeding Process of male sterile line and maintainer.
Summary of the invention
The technical problem to be solved in the present invention is the defect existing according to prior art, proposes a kind of method of quick acquisition rouge radish cytoplasm male sterility line and maintainer, and the method can significantly save work and economize this, greatly shortens breeding time.
Technical scheme of the present invention realizes by following steps:
(1) collect in a large number dissimilar rouge radish germ plasm resource, be seeded into experimental field in late August-early September, within whole breeding time, observe tree characteristics and fleshy root pigment content situation, and according to breeding goals such as blade profile, root type, fleshy root pigment contents, the directed individual plant of selecting, carries out selfed breeding.
(2) in self progeny colony, select the individual plant that fleshy root pigment content is high, root type is larger to continue selfing, purify, and according to the directed individual plant of selecting of objective trait.
(3) according to breeding goal, find the Ogura cytoplasmic male sterility system approaching with objective trait as maternal, as male parent, carry out middle intermolecular hybrid with the good individual plant of directive breeding, proterties, obtain hybrid F1, thereby the sterile gene of male sterile line is transferred on rouge radish.
(4), in the cytoplasmic male sterilty hybrid plant colony of transformation, screening as female parent, continues biparental cross with directive breeding in self progeny, individual plant that proterties is good as male parent with the immediate sterile strain of male parent target; Self progeny continues selfing purification simultaneously.
(5) self progeny's purification and the transformation of male sterile line, in per generation, all carries out in pairs simultaneously, through selfing purification and the male sterile line transformation in 6-7 generation, can obtain cytoplasm male sterility line and maintainer simultaneously; And maintainer has the proterties of good stable, can be used as the parent material of other crossbreed of preparation.
(6) the cytoplasm male sterility line material obtaining taking institute's transformation is as maternal, and choosing the rouge radish selfings similar to female parent such as botany proterties is stable, root type, blade profile is male parent, preparing hybrid kind.
In order to reach better effect, preferably:
1, described resource is from various parts of the country, has both comprised that local farmers', from reserving seed for planting, also comprises the crossbreed of seed selection, and the proterties such as its plant type, blade profile, fleshy root shape and size, anthocyanidin content differ greatly, and mix serious.
2, described rouge radish inbred line breeding target is: blade profile is plate leaf or decomposite leaf, and root type is straight, larger, and botany proterties is stable, coordinate force is good, pollen amount is large, the most important thing is that fleshy root skin and meat portion haematochrome content are higher than 140mg/100g fresh weight.
3, the breeding goal of described male sterile line is: sterile plant rate and sterile degree are 100%, and heredity of male sterility is stable, is not subject to the impact of environmental condition; Flower bud development is normal, corolla and petal normal, and it is shrivelled, fine that Anther degeneration becomes, and stamen is significantly shorter than style; Nectary size, quantity and honey amount are physically well developed; Gynoecium structure is normal, can normally set seeds.
4,, in described backcross transformation hybrid population, select the approaching individual plant of the proterties such as plant type, blade profile, root type, pigment content and male parent to reserve seed for planting.
5, described when selfing purify proceed to 4-5 for time, tree characteristics no longer separates, then selfing 1-2 generation, can obtain the stable inbred line of isozygotying, and is also maintainer.
6, described 6-7 for backcross transformation cytoplasm male sterility line proterties and maintainer in full accord, its sterile plant rate reaches 100%.
7, described in the time blooming by pinching, the mode of pinching regulates parents to be consistent at the florescence, and in every mu of seed farm, puts into 1-2 case honeybee and carry out supplementary pollination, at least 1500m of the isolation distance of seed farm.
Beneficial effect of the present invention is:
1, purify through inbreeding of more generation, obtained that a large amount of dissimilar botany proterties are stable, coordinate force is good, radish root skin and meat merocyanine cellulose content and the high rouge radish inbred line of output;
2, through 6-7 for continuous backcross transformation, the male sterile line of acquisition has sterile plant rate and sterile degree is 100%, heredity of male sterility is stable; Flower bud development is normal, corolla and petal normal, and it is shrivelled, fine that Anther degeneration becomes, and stamen is significantly shorter than style; Nectary size, quantity and honey amount are physically well developed; Gynoecium structure is normal, the feature such as can normally set seeds;
The crossbreed of the male sterile line that 3, utilization obtains and the preparation of other inbred line, hybrid vigour is remarkable, botany proterties neat and consistent, sterility is thorough; Saving of labor's abridged edition, has shortened breeding time greatly, has improved breeding efficiency, and can effectively prevent the outflow of specific gene resource, contributes to the protection of independent IP.
Brief description of the drawings
Fig. 1 is Breeding Process schematic diagram of the present invention.
Embodiment
Embodiment 1
Select the farmers' that is derived from Jiangsu from reserving seed for planting " northern Suzhou carrot ", plate blade profile, fleshy root skin and meat portion haematochrome rich content; Choose Ogura cytoplasmic male sterility based material D-1 as sterile source, plate blade profile, the white heart of red skin.
1, rouge radish kind " northern Suzhou carrot " after planting, after First selfed progeny, observing characters of progenies separates and changes, according to the breeding goal that plate blade profile, fleshy root skin and meat portion pigment content are abundant, output is high, pollen amount is many, compatibility is good, the directed individual plant of selecting is reserved seed for planting, and carries out second generation selfing.
2,, in selfing 2 generation colony, according to breeding goal, continue the directed individual plant selfing of selecting and purify, and the directed individual plant of selecting carries out third generation selfing.
3, simultaneously, according to breeding goal, find the same kind Ogura cytoplasmic male sterility based material D-1 approaching with objective trait as female parent, select individual plant with the rouge radish in 2 generations of selfing and make male parent, hybridize, obtain hybrid F1, thereby the sterile gene of male sterile line is transferred on rouge radish.
4, rouge radish self progeny continues selfing purification; In cytoplasm male sterility line transformation hybrid plant colony, screening is made female parent with the immediate sterile strain of breeding goal, continues biparental cross with the individual plant of directive breeding in self progeny simultaneously.
5, " northern Suzhou carrot " self progeny's purification and the backcross transformation of cytoplasm male sterility line, in per generation, all carries out in pairs simultaneously, when selfing proceeds to 4-5 for not having proterties obviously to separate with backcrossing, selfing and the 1-2 generation that backcrosses again, can obtain the rouge radish with objective trait and isozygoty and stablize inbred line and cytoplasm male sterility line, inbred line is also maintainer simultaneously.
6,6-7 has sterile plant rate for the rouge radish cytoplasm male sterility line of backcross transformation and sterile strain degree all reaches 100%, and heredity of male sterility is stable, is not subject to environmental influence; Development of floral organs is normal, and it is shrivelled, fine that Anther degeneration becomes, and stamen is significantly shorter than style, and nectary development is sound; Gynoecium structure is normal, can normally be pollinated and set seeds.
7, can educate rouge radish hybridization between selfed lines as maternal with other excellence taking the male sterile based material obtaining, preparing hybrid kind, when the production of hybrid seeds by pinching, the mode of pinching regulates parents to be consistent at the florescence, and in every mu of seed farm, put into 1-2 case honeybee and carry out supplementary pollination, at least 1500m of the isolation distance of seed farm.
8, maintainer (inbred line) the important character comparison that the cytoplasm male sterility line that transformation obtains and inbreeding of more generation obtain, in table 1.
The comparison of table 1 major traits
Embodiment 2
Select the commodity kind " carmine No. 1 " that is derived from Sichuan, decomposite leaf type, fleshy root skin and meat portion haematochrome rich content; Choose Ogura cytoplasmic male sterility based material C-1 as sterile source, decomposite leaf type, the Bai Pibai heart.
1, rouge radish kind " carmine No. 1 " after planting, after First selfed progeny, observing characters of progenies separates and changes, according to the breeding goal that decomposite leaf type, fleshy root skin and meat portion pigment content are abundant, output is high, pollen amount is many, compatibility is good, the directed individual plant of selecting is reserved seed for planting, and carries out second generation selfing.
2,, in selfing 2 generation colony, according to breeding goal, continue the directed individual plant selfing of selecting and purify, and the directed individual plant of selecting carries out third generation selfing.
3, simultaneously, according to breeding goal, find the same kind Ogura cytoplasmic male sterility based material D-1 approaching with objective trait as female parent, select individual plant with the rouge radish in 2 generations of selfing and make male parent, hybridize, obtain hybrid F1, thereby the sterile gene of male sterile line is transferred on rouge radish.
4, rouge radish self progeny continues selfing purification; In cytoplasm male sterility line transformation hybrid plant colony, screening is made female parent with the immediate sterile strain of breeding goal, continues biparental cross with the individual plant of directive breeding in self progeny simultaneously.
5, " carmine No. 1 " self progeny's purification and backcross transformation of cytoplasm male sterility line, in per generation, all carries out in pairs simultaneously, when selfing proceeds to 4-5 for not having proterties obviously to separate with backcrossing, selfing and the 1-2 generation that backcrosses again, can obtain the rouge radish with objective trait and isozygoty and stablize inbred line and cytoplasm male sterility line, inbred line is also maintainer simultaneously.
6,6-7 has sterile plant rate for the rouge radish cytoplasm male sterility line of backcross transformation and sterile strain degree all reaches 100%, and heredity of male sterility is stable, is not subject to environmental influence; Development of floral organs is normal, and it is shrivelled, fine that Anther degeneration becomes, and stamen is significantly shorter than style, and nectary development is sound; Gynoecium structure is normal, can normally be pollinated and set seeds.
7, can educate rouge radish hybridization between selfed lines as maternal with other excellence taking the male sterile based material obtaining, preparing hybrid kind, when the production of hybrid seeds by pinching, the mode of pinching regulates parents to be consistent at the florescence, and in every mu of seed farm, put into 1-2 case honeybee and carry out supplementary pollination, at least 1500m of the isolation distance of seed farm.
8, maintainer (inbred line) important character that the cytoplasm male sterility line that transformation obtains and inbreeding of more generation obtain is relatively in table 2.
The comparison of table 2 important character
Claims (8)
1. a breeding method that obtains fast rouge radish cytoplasm male sterility line and maintainer, is characterized in that, comprises the following steps:
(1) collect in a large number dissimilar rouge radish germ plasm resource, be seeded into experimental field in late August-early September, within whole breeding time, observe tree characteristics and fleshy root pigment content situation, and according to blade profile, root type, fleshy root pigment content breeding goal, the directed individual plant of selecting, carries out selfed breeding;
(2) in self progeny colony, select the individual plant that fleshy root pigment content is high, root type is larger to continue selfing, purify, and according to the directed individual plant of selecting of objective trait;
(3) according to breeding goal, find with the approaching Ogura cytoplasmic male sterility of objective trait system as maternal, as male parent, carry out middle intermolecular hybrid with the good individual plant of directive breeding, proterties, obtain hybrid F1, thereby the sterile gene of male sterile line is transferred on rouge radish;
(4), in the cytoplasmic male sterilty hybrid plant colony of transformation, screening as female parent, continues biparental cross with directive breeding in self progeny, individual plant that proterties is good as male parent with the immediate sterile strain of male parent target; Self progeny continues selfing purification simultaneously;
(5) self progeny's purification and the transformation of male sterile line, in per generation, all carries out in pairs simultaneously, through selfing purification and the male sterile line transformation in 6-7 generation, can obtain cytoplasm male sterility line and maintainer simultaneously;
(6) the cytoplasm male sterility line material obtaining taking institute transformation is maternal, chooses that botany proterties is stable, root type, the blade profile rouge radish selfing similar with female parent be male parent, and preparing hybrid kind is as filial generation.
2. obtain fast according to claim 1 the breeding method of rouge radish cytoplasm male sterility line and maintainer, it is characterized in that collected resource is from various parts of the country, both comprised that local farmers' was from reserving seed for planting, also comprise the crossbreed of seed selection, its plant type, blade profile, fleshy root shape and size, anthocyanidin content proterties differ greatly, and mix serious.
3. according to the breeding method that obtains fast rouge radish cytoplasm male sterility line and maintainer described in claim 1 or 2, it is characterized in that rouge radish inbred line breeding target is: blade profile is plate leaf or decomposite leaf, root type is straight, larger, botany proterties is stable, coordinate force is good, pollen amount is large, and fleshy root skin and meat portion haematochrome content are higher than 140mg/100g fresh weight.
4. obtain fast according to claim 3 the breeding method of rouge radish cytoplasm male sterility line and maintainer, the breeding goal that it is characterized in that male sterile line is: sterile plant rate and sterile degree are 100%, heredity of male sterility is stable, is not subject to the impact of environmental condition; Flower bud development is normal, and corolla and petal are normal, and it is shrivelled, fine that Anther degeneration becomes, and stamen is significantly shorter than style; Nectary size, quantity and honey amount are physically well developed; Gynoecium structure is normal, can normally set seeds.
5. according to the breeding method that obtains fast rouge radish cytoplasm male sterility line and maintainer described in claim 1,2 or 4, it is characterized in that in backcross transformation hybrid population, select the approaching individual plant of plant type, blade profile, root type, pigment content proterties and male parent to reserve seed for planting.
6. the breeding method of a kind of quick acquisition rouge radish cytoplasm male sterility line and maintainer according to claim 5, it is characterized in that when selfing purify proceed to 4-5 for time, tree characteristics no longer separates, then selfing 1-2 generation, can obtain the stable inbred line of isozygotying, be also maintainer.
7. according to the breeding method of a kind of quick acquisition rouge radish cytoplasm male sterility line and maintainer described in claim 1,2,4 or 6, it is characterized in that 6-7 for backcross transformation cytoplasm male sterility line proterties and maintainer in full accord, its sterile plant rate reaches 100%.
8. obtain fast according to claim 7 the breeding method of rouge radish cytoplasm male sterility line and maintainer, characterized by further comprising in the time blooming by pinching, the mode of pinching regulates parents to be consistent at the florescence, and in every mu of seed farm, put into 1-2 case honeybee and carry out supplementary pollination, at least 1500m of the isolation distance of seed farm.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN105961192A (en) * | 2016-06-07 | 2016-09-28 | 吉林省农业科学院 | Method for breeding sorghum sterile line through stabilizing and backcrossing method |
| CN110896849A (en) * | 2019-12-12 | 2020-03-24 | 重庆市渝东南农业科学院 | High-sterility-based carmine radish seed cultivation method |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN105961192A (en) * | 2016-06-07 | 2016-09-28 | 吉林省农业科学院 | Method for breeding sorghum sterile line through stabilizing and backcrossing method |
| CN105961192B (en) * | 2016-06-07 | 2018-05-04 | 吉林省农业科学院 | A kind of method using the Backcrossing methods transformation Chinese sorghum sterile line when stablizing |
| CN110896849A (en) * | 2019-12-12 | 2020-03-24 | 重庆市渝东南农业科学院 | High-sterility-based carmine radish seed cultivation method |
| CN110896849B (en) * | 2019-12-12 | 2021-08-06 | 重庆市渝东南农业科学院 | High-sterility-based carmine radish seed cultivation method |
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Effective date of registration: 20201126 Address after: Li Xin Zhuang Zhen Cui Tang Cun, Shan county, Heze City, Shandong Province Patentee after: Shanxian Qiuge Agricultural Products Co.,Ltd. Address before: 610000 room 1406, floor 14, unit 1, building 5, No. 366, South Ronghua Road, hi tech Zone, Chengdu, Sichuan Province Patentee before: Sichuan qiyibiao Information Technology Co.,Ltd. Effective date of registration: 20201126 Address after: 610000 room 1406, floor 14, unit 1, building 5, No. 366, South Ronghua Road, hi tech Zone, Chengdu, Sichuan Province Patentee after: Sichuan qiyibiao Information Technology Co.,Ltd. Address before: No. 112 Ninghang Road, Jurong City, Jiangsu Province, 212400 Patentee before: JIANGSU FOOTHILL ZHENJIANG AGRICULTURE Research Institute |
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| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20160706 Termination date: 20210812 |