CN104160946B - Breeding method for cytoplasmic male sterility line and maintainer line of brassica oleracea - Google Patents
Breeding method for cytoplasmic male sterility line and maintainer line of brassica oleracea Download PDFInfo
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- CN104160946B CN104160946B CN201410370102.2A CN201410370102A CN104160946B CN 104160946 B CN104160946 B CN 104160946B CN 201410370102 A CN201410370102 A CN 201410370102A CN 104160946 B CN104160946 B CN 104160946B
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Abstract
The invention discloses a breeding method for cytoplasmic male sterility line and maintainer line of brassica oleracea and application. The method mainly comprises: selecting different types of brassica oleracea materials, inbreeding to obtain a first generation and further to determine a breeding target, directionally selecting a single strain and performing second-generation inbreeding; directionally selecting a single strain from the inbred progeny colony and continuing to perform inbreeding, and directionally selecting a single strain according to the breeding target; also selecting hard-flower type brassica oleracea cytoplasmic male sterility line as a female parent and using the selected single strain as a male parent to perform interspecific hybridization, so as to obtain a hybrid F1 and transfer the CMS gene of the sterile line into brassica oleracea; continuing to perform selfing purification on the inbred progeny, and also screening out a sterile strain closest to the male parent breeding target from the cytoplasmic male sterility line hybrid plant colony and taking the sterile strain as a male parent, taking a single strain closest to the breeding target and screened out from the selfing progeny as a female parent, and continuing to perform paired hybridization; and performing purification of the inbred progeny and transformation of the male sterilty line on each generation at the same time in a paired way, so as to obtain the maintainer line and the cytoplasmic male sterility line after a plurality of generations are obtained through inbreeding. The method is capable of substantially saving labor and saving cost, and shortening breeding time.
Description
Technical field
The present invention relates to the breeding method of a kind of Flos pini dish, especially obtain Flos pini dish cytoplasm male sterility line and
Keep the breeding method of system, belong to field of plant variety breeding technology.
Background technology
Compared with Flos pini dish (abbreviation of bouquet loose type Brassica oleracea L. var. botrytis L.) bouquet more close with common Brassica oleracea L. var. botrytis L.,
Bouquet is loose, bennet length, green, tastes sweeter, crisp, is difficult to well-done when stir-frying and eating, bennet color after heating
Become greener；Dehydration adds man-hour, and based on edible bennet, stalk color is dark green, Flos pini dried vegetable unique flavor, and
Nutritious, deeply liked by consumer.By the analysis to Flos pini dish Main Nutrient Quality, chlorophyll content
12.8μg·g-1(FW), carotenoid content 3.3 μ g g-1(FW), VC content 629mg kg-1、
Soluble sugar content 2.82%, with tight type Brassica oleracea L. var. botrytis L. ratio, chlorophyll content is high 2.7 times, class trailing plants recklessly
Foretelling cellulose content high 1.5 times, VC exceeds 40%, and soluble sugar exceeds nearly 10%, but moisture, crude fibre,
Crude protein aspect is without significant difference (the Changjiang river vegetable, 2012 (20): 37-39).The mouthfeel that Flos pini dish is excellent
And nutritional characteristic means that Flos pini dish industry will have huge development prospect, Substitute For Partial is tightly spent type
Consumption and industry demand.Flos pini dish plants consumption as far back as Fujian and Taiwan, in recent years from area, Zhejiang, Fujian
Spread to China North China, Central China rapidly.Under China's Flos pini dish major producing area is distributed in the Changjiang river at present
Trip, Fujian, Zhejiang, alpine region, Hubei Province, Lanzhou, Bashang plateau area, and North China, planting area is wide
General Relatively centralized again, has basically formed the production pattern in Flos pini dish year-round supply market, and cultivated area has reached 2
Ten thousand hm2Above, accounting for Brassica oleracea L. var. botrytis L. and produce the 10% of area, the author predicts, a few years from now on Flos pini dish will at home
Extensively plantation, and one of main Types of Brassica oleracea L. var. botrytis L. consumption will be become, cultivated area will reach 100,000 hm2Above,
Development potentiality is huge.
Flos pini dish Heterosis is obvious, and the aspect such as yield, quality and resistance all shows hybrid vigor.
Research shows, utilizes cytoplasm male sterility line preparing hybrid kind, has method simplicity, genetic stability, miscellaneous
Friendship rate is high, time saving and energy saving, sterility advantage thoroughly, and can effectively control the outflow of merit gene,
In terms of Flos pini dish cross-breeding, there is significant application value.And cytoplasmic male sterility (CMS) is a kind of natural
Phenomenon, shows as maternal inheritance, pollen abortion and gynoecium normal, with the pollen of fertile plant to sterile plant gynoecium
Pollination, can be the most solid.Bouquet trophosome mainly gathered in the crops by Flos pini dish, rather than obtains seed, so in hybridization
Need not restorer during the production of hybrid seeds, therefore obtain Flos pini dish cytoplasm male sterility line and keep system be utilize male not
Educate the key of preparing hybrid kind.
Summary of the invention
The technical problem to be solved in the present invention is the defect existed according to prior art, proposes a kind of Flos pini dish born of the same parents
Matter male sterility line and the breeding method of holding system, the method can significantly save work and save this, be greatly shortened breeding
Time.
Technical scheme is realized by following steps:
(1) dissimilar Flos pini dish germ plasm resource is collected, observation of plant character, anti-in whole period of duration
Characteristic of disease, yielding ability etc., determine breeding goal, orthoselection individual plant, carry out selfed breeding.
(2) select disease resistance stalk good, blue or green Flos pini menu strain to continue selfing in self progeny colony, purify,
And according to objective trait orthoselection individual plant.
(3) female parent is made with hard flower pattern Herba Cleomis cytoplasm male sterility line, with directive breeding, list that character is excellent
Male parent is made in strain, carries out middle intermolecular hybrid, it is thus achieved that Hybrids F1, thus the sterile gene of sterile line is transferred to Flos pini
On dish.
(4) in cytoplasmic male sterilty hybrid plant colony, screening is made with the immediate sterile plant of male parent target
Paired cross is continued as male parent for directive breeding in female parent, with self progeny, individual plant that character is excellent；With
Time self progeny continue selfing purify.
(5) purification of self progeny and the transformation of male sterility line, per in generation, is carried out, all in pairs through 6-7 simultaneously
The selfing purification in generation and sterile line transformation, can obtain cytoplasm male sterility line simultaneously and keep system；And keep
Cording has the character of good stable, can be as the parent material preparing other cenospecies.
(6) the cytoplasm male sterility line material obtained with institute transformation is as female parent, choose Agronomic trait stable,
The good selfing line similar to female parent of disease resistance is male parent, preparing hybrid kind.
In order to reach more preferable effect, preferably:
1, described Flos pini dish inbred line breeding target is: bouquet is pure white, and bennet is dark green, and disease resistance is good, plant
Character is stable, combining ability good, pollen amount is big.
2, the breeding goal of described male sterility line is: sterile plant rate and sterile degree are 100%, sterility
Inheritance stability, the most influenced by environmental conditions；Flower bud development is normal, and corolla and petal are normal, flower pesticide
Being degenerated to shrivelled, fine, stamen is significantly shorter than style；Nectary size, quantity and honey amount are physically well developed；Female
Stamen structure is normal, it is possible to normally set seeds.
3, in described backcross transformation hybrid population, plant type, blade profile, bouquet shape, color, disease resistance are selected
The individual plant close etc. character and male parent is reserved seed for planting.
4, described when selfing purify proceed to 4-5 for time, tree characteristics no longer separates, then selfing 1-2 generation,
The stable selfing line that isozygotys can be obtained, also for keeping system.
5, described 6-7 for backcross transformation cytoplasm male sterility line character be completely the same with holding, it is not
Educate strain rate and reach 100%.
6, described by advance or postpone cut bouquet by the way of regulate parents' florescence and keep unanimously, and every mu of system
Kind Tanaka puts into 1-2 case Apis and carries out supple-mentary pollination, the isolation distance of seed farm at least 1500m.
The invention have the benefit that
1, purify through inbreeding of more generation, it is thus achieved that a large amount of dissimilar Agronomic trait are stable, combining ability good, flower
The selfing line that ball character is excellent；
2, through 6-7 for continuous backcross transformation, it is thus achieved that sterile line there is sterile plant rate and sterile degree is 100%,
Heredity of male sterility is stable；Flower bud development is normal, and corolla and petal are normal, and Anther degeneration becomes shrivelled, fine
Thin, stamen is significantly shorter than style；Nectary size, quantity and honey amount are physically well developed；Gynoecium structure is normal, energy
Reach features such as normally setting seeds；
3, utilizing the cenospecies of sterile line and other selfing line preparation obtained, hybrid vigor is notable, botany
Character neat and consistent, sterility is thorough；Dart transfer, substantially reduces breeding time, improves breeding effect
Rate, and can effectively prevent specific gene resource from outflowing, contribute to the protection of independent IP.
Accompanying drawing explanation
Fig. 1 is the Breeding Process schematic diagram of the present invention.
Detailed description of the invention
Embodiment 1
1) selection is derived from the Flos pini vegetable kind in Taiwan, and its feature is blue or green stalk, late-maturing；Choose hard flower pattern Herba Cleomis
Cytoplasm male sterility line material B-1 is as sterile source.
2) observe characters of progenies after First selfed progeny to separate and change, according to plant type, bouquet shape, blue or green stalk,
Breeding goals disease-resistant, that high yield, pollen amount are many, affinity is good, orthoselection individual plant is reserved seed for planting, and carries out second
For selfing.
3) in selfing 2 generation colony, according to breeding goal, continue orthoselection individual plant selfing and purify, and fixed
Third generation selfing is carried out to selecting individual plant.
4) with cytoplasm male sterility line material B-1 as female parent while, selected single with the Flos pini dish in selfing 2 generation
Male parent is made in strain, hybridizes, it is thus achieved that Hybrids F1, thus is transferred on Flos pini dish by the sterile gene of sterile line.
5) self progeny continues selfing purification；Simultaneously in cytoplasm male sterility line transformation hybrid plant colony,
Screening makees female parent with the immediate sterile plant of breeding goal, continues into the individual plant of directive breeding in self progeny
To hybridization.
6) purification of Flos pini dish self progeny and the backcross transformation of cytoplasm male sterility line, per in generation, is all in pairs simultaneously
Carry out, do not have character to be clearly separated when selfing and backcrossing proceeds to 4-5 generation, then selfing and the 1-2 generation that backcrosses,
Can obtain and there is the Flos pini dish of objective trait isozygoty stable selfing line and cytoplasm male sterility line, selfing line with
Time be also to maintain.
7) 6-7 has sterile plant rate for the Flos pini dish cytoplasm male sterility line of backcross transformation and sterile plant degree all reaches
To 100%, and heredity of male sterility is stable, not by environmental influence；Development of floral organs is normal, Anther degeneration
Becoming shrivelled, fine, stamen is significantly shorter than style, and nectary development perfects；Gynoecium structure is normal, can normally be subject to
Powder is set seeds.
8) the Flos pini dish hybridization between selfed lines can educated for maternal and other excellence with the male sterility based material obtained,
Preparing hybrid kind, regulating parents' florescence during the production of hybrid seeds by the way of cutting ball time keeps unanimously, and often
Mu seed farm puts into 1-2 case Apis and carries out supple-mentary pollination, the isolation distance of seed farm at least 1500m.
The sterile plant rate of the cytoplasm male sterility line that result transformation obtains and sterile degree are 100%, and sterility is lost
Pass stable；Flower bud development is normal, and corolla and petal are normal, and Anther degeneration becomes shrivelled, fine, stamen
It is significantly shorter than style；Nectary size, quantity and honey amount are physically well developed；Gynoecium structure is normal, it is possible to normally tie
Seed；Blue or green stalk, high antiviral are sick, late-maturing, period of duration about 90 days；The holding system that inbreeding of more generation obtains is (certainly
Hand over system) major traits be: blue or green stalk, high antiviral is sick, and inheritance stability is late-maturing, period of duration about 90 days.
Embodiment 2
Except step 1) select the commodity kind being derived from Japan, blue or green stalk is spent in vain, precocious, and high antiviral is sick；With firmly
Flower pattern Herba Cleomis cytoplasm male sterility line material B-1 is outside sterile source, and remaining operation is with embodiment 1.
The sterile plant rate of the cytoplasm male sterility line that result transformation obtains and sterile degree are 100%, and sterility is lost
Pass stable；Flower bud development is normal, and corolla and petal are normal, and Anther degeneration becomes shrivelled, fine, stamen
It is significantly shorter than style；Nectary size, quantity and honey amount are physically well developed；Gynoecium structure is normal, it is possible to normally tie
Seed；Blue or green stalk, high antiviral are sick, precocious, period of duration about 50 days；The holding system that inbreeding of more generation obtains is (certainly
Hand over system) major traits be: blue or green stalk, high antiviral is sick, and inheritance stability is precocious, period of duration about 50 days.
Embodiment 3
Except step 1) select the commodity kind being derived from China, blue or green stalk to spend in vain, in ripe, high antiviral is sick；With firmly
Flower pattern Herba Cleomis cytoplasm male sterility line material B-1 is outside sterile source, and remaining operation is with embodiment 1.
The sterile plant rate of the cytoplasm male sterility line that result transformation obtains and sterile degree are 100%, and sterility is lost
Pass stable；Flower bud development is normal, and corolla and petal are normal, and Anther degeneration becomes shrivelled, fine, stamen
It is significantly shorter than style；Nectary size, quantity and honey amount are physically well developed；Gynoecium structure is normal, it is possible to normally tie
Seed；Blue or green stalk, high antiviral are sick, in ripe, period of duration about 70 days；The holding system that inbreeding of more generation obtains is (certainly
Hand over system) major traits be: blue or green stalk, high antiviral is sick, inheritance stability, in ripe, period of duration about 70 days.
Claims (2)
1. a Flos pini dish cytoplasm male sterility line and keep the breeding method of system, it is characterised in that include with
Lower step:
(1) dissimilar Flos pini dish germ plasm resource is collected, observation of plant character, anti-in whole period of duration
Characteristic of disease, yielding ability, determine breeding goal, orthoselection individual plant, carry out selfed breeding；
(2) select disease resistance stalk good, blue or green Flos pini menu strain to continue selfing in self progeny colony, purify,
And according to objective trait orthoselection individual plant；
(3) female parent is made with hard flower pattern Herba Cleomis cytoplasm male sterility line, with directive breeding, list that character is excellent
Male parent is made in strain, carries out middle intermolecular hybrid, it is thus achieved that Hybrids F1, thus the sterile gene of sterile line is transferred to Flos pini
On dish；
(4) in cytoplasmic male sterilty hybrid plant colony, screening is made with the immediate sterile plant of male parent target
Paired cross is continued as male parent for directive breeding in female parent, with self progeny, individual plant that character is excellent；With
Time self progeny continue selfing purify；
(5) purification of self progeny and the transformation of male sterility line, per in generation, is carried out, all in pairs through 6-7 simultaneously
The selfing purification in generation and sterile line transformation, just can obtain cytoplasm male sterility line simultaneously and keep system；
(6) the cytoplasm male sterility line material obtained with institute transformation is as female parent, choose Agronomic trait stable,
The good selfing line similar to female parent of disease resistance is male parent, and preparing hybrid kind is as filial generation；Described hybridization
In offspring, the individual plant selecting plant type, blade profile, bouquet shape, color, disease resistance close with male parent is reserved seed for planting；
Wherein, the breeding goal of Flos pini dish selfing line is: bouquet is pure white, and bennet is dark green, and disease resistance is good, plants
Thing character is stable, combining ability good, pollen amount is big；
The breeding goal of described male sterility line is: sterile plant rate and sterile degree are 100%, heredity of male sterility
Stable, the most influenced by environmental conditions；Flower bud development is normal, and corolla and petal are normal, Anther degeneration
Becoming shrivelled, fine, stamen is significantly shorter than style；Nectary size, quantity and honey amount are physically well developed；Gynoecium is tied
Structure is normal, it is possible to normally set seeds；
Described breeding method also include by advance or postpone cut bouquet by the way of regulate parents' florescence and keep one
Causing, and put into 1-2 case Apis in every mu of seed farm and carry out supple-mentary pollination, the isolation distance of seed farm is at least
1500m。
The most according to claim 1, Flos pini dish cytoplasm male sterility line and the breeding method of holding system, it is special
Levy and be, selfing described in step (5) purify proceed to 4-5 for time, tree characteristics no longer separates, then
In selfing 1-2 generation, just can obtain the stable selfing line that isozygotys, also for keeping system.
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| CN107347626A (en) * | 2017-07-05 | 2017-11-17 | 张家港市松田创新农业科技有限公司 | A kind of preserved egg dish cultural method |
| CN107581055A (en) * | 2017-08-29 | 2018-01-16 | 云南省农业科学院热区生态农业研究所 | A kind of breeding method of blue or green stalk preserved egg dish |
| CN110301349B (en) * | 2019-08-12 | 2021-09-14 | 浙江省农业科学院 | Breeding method of green pagoda cauliflower hybrid |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1565170A (en) * | 2003-07-02 | 2005-01-19 | 天津科润农业科技股份有限公司蔬菜研究所 | Hybrid cauliflower cultivation method utilizing male sterility line |
| CN103190336A (en) * | 2013-04-03 | 2013-07-10 | 镇江瑞繁农艺有限公司 | Method for transforming wild cabbage into male sterility line of kohlrabi and breeding hybrids |
| CN103461105A (en) * | 2013-09-11 | 2013-12-25 | 镇江瑞繁农艺有限公司 | Method for breeding male sterile line and maintainer line of kale |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| NL1003239C2 (en) * | 1996-05-31 | 1997-12-03 | Bejo Zaden Bv | Cytoplasmic male sterile Brassica oleracea plant, as well as a method for obtaining such a plant. |
| CA2628656C (en) * | 2005-11-22 | 2016-05-17 | Franciscus Van Den Bosch | Broccoli type having curds with detached florets |
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Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1565170A (en) * | 2003-07-02 | 2005-01-19 | 天津科润农业科技股份有限公司蔬菜研究所 | Hybrid cauliflower cultivation method utilizing male sterility line |
| CN103190336A (en) * | 2013-04-03 | 2013-07-10 | 镇江瑞繁农艺有限公司 | Method for transforming wild cabbage into male sterility line of kohlrabi and breeding hybrids |
| CN103461105A (en) * | 2013-09-11 | 2013-12-25 | 镇江瑞繁农艺有限公司 | Method for breeding male sterile line and maintainer line of kale |
Non-Patent Citations (2)
| Title |
|---|
| Improvement of Cauliflower Male Sterile Lines with Brassica Nigra Cytoplasm, Phenotypic Expression and Possibility of Practical Application;P. Kamiński et.al.,;《Journal of Agricultural Science》;20120202;第4卷(第4期);第190-200页 * |
| 花椰菜雄性不育系的选育与应用;陈文辉等;《福建农业学报》;20101231;第25卷(第5期);第580-583页 * |
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Effective date of registration: 20201223 Address after: 430000 Shunmin Building, No. 36 Wuer Road, Wuchang District, Wuhan City, Hubei Province, No. 3, Unit 19, No. 2 Patentee after: Wuhan Asia-Africa Seed Industry Co.,Ltd. Address before: 212400 NInghang Road 112, Jurong, Jiangsu Patentee before: JIANGSU FOOTHILL ZHENJIANG AGRICULTURE Research Institute |