CN103201376A - 用于生产丁醇的酵母生产培养物 - Google Patents

用于生产丁醇的酵母生产培养物 Download PDF

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Publication number
CN103201376A
CN103201376A CN2011800298462A CN201180029846A CN103201376A CN 103201376 A CN103201376 A CN 103201376A CN 2011800298462 A CN2011800298462 A CN 2011800298462A CN 201180029846 A CN201180029846 A CN 201180029846A CN 103201376 A CN103201376 A CN 103201376A
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yeast
culture
production
butanols
butanol
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CN2011800298462A
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Chinese (zh)
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V.纳加拉彦
M.G.布拉穆奇
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Butamax Advanced Biofuels LLC
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Butamax Advanced Biofuels LLC
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14—Fungi; Culture media therefor
    • C12N1/16—Yeasts; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14—Fungi; Culture media therefor
    • C12N1/145—Fungi isolates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14—Fungi; Culture media therefor
    • C12N1/16—Yeasts; Culture media therefor
    • C12N1/18—Baker's yeast; Brewer's yeast
    • C12N1/185—Saccharomyces isolates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/01—Preparation of mutants without inserting foreign genetic material therein; Screening processes therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/16—Butanols
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/645—Fungi ; Processes using fungi
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/645—Fungi ; Processes using fungi
    • C12R2001/85—Saccharomyces
    • C12R2001/865—Saccharomyces cerevisiae
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/10—Biofuels, e.g. bio-diesel

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Mycology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • Virology (AREA)
  • Botany (AREA)
  • Medicinal Chemistry (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Molecular Biology (AREA)
  • Plant Pathology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
CN2011800298462A 2010-06-17 2011-06-16 用于生产丁醇的酵母生产培养物 Pending CN103201376A (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US35573310P 2010-06-17 2010-06-17
US61/355,733 2010-06-17
PCT/US2011/040697 WO2011159894A1 (en) 2010-06-17 2011-06-16 Yeast production culture for the production of butanol

Publications (1)

Publication Number Publication Date
CN103201376A true CN103201376A (zh) 2013-07-10

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CN2011800298462A Pending CN103201376A (zh) 2010-06-17 2011-06-16 用于生产丁醇的酵母生产培养物

Country Status (12)

Country Link
US (1) US20120149080A1 (enExample)
EP (1) EP2582786A1 (enExample)
JP (1) JP2013528398A (enExample)
KR (1) KR20130027551A (enExample)
CN (1) CN103201376A (enExample)
AU (1) AU2011268326B2 (enExample)
BR (1) BR112012031847A2 (enExample)
CA (1) CA2801576A1 (enExample)
MX (1) MX2012014550A (enExample)
NZ (1) NZ603546A (enExample)
WO (1) WO2011159894A1 (enExample)
ZA (1) ZA201208575B (enExample)

Families Citing this family (29)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8945899B2 (en) 2007-12-20 2015-02-03 Butamax Advanced Biofuels Llc Ketol-acid reductoisomerase using NADH
NZ579780A (en) 2007-04-18 2012-07-27 Butamax Advanced Biofuels Llc Fermentive production of isobutanol using highly active ketol-acid reductoisomerase enzymes
US8188250B2 (en) 2008-04-28 2012-05-29 Butamax(Tm) Advanced Biofuels Llc Butanol dehydrogenase enzyme from the bacterium Achromobacter xylosoxidans
CN102186983A (zh) 2008-09-29 2011-09-14 布特马斯先进生物燃料有限责任公司 酵母中提高的异源Fe-S酶活性
MX2012007583A (es) 2009-12-29 2012-07-30 Butamax Tm Advanced Biofuels Alcohol deshidrogenasas (adh) utiles para la produccion fermentativa de alcoholes alquilicos de cadena corta.
CN102782119B (zh) 2010-02-17 2016-03-16 布特马斯先进生物燃料有限责任公司 改善需Fe-S簇蛋白质的活性
NZ707800A (en) 2010-09-07 2016-09-30 Butamax Tm Advanced Biofuels Integration of a polynucleotide encoding a polypeptide that catalyzes pyruvate to acetolactate conversion
WO2012125688A2 (en) 2011-03-14 2012-09-20 Easel Biotechnologies, Llc Microbial syntheses of aldehydes and corresponding alcohols
EP2689014B1 (en) 2011-03-24 2019-04-24 Butamax (TM) Advanced Biofuels LLC Host cells and methods for production of isobutanol
CN105473726B (zh) 2011-07-28 2021-03-12 布特马斯先进生物燃料有限责任公司 酮异戊酸脱羧酶及其使用方法
WO2013067325A1 (en) 2011-11-03 2013-05-10 Easel Biotechnologies, Llc Microbial production of n-butyraldehyde
AU2012347687A1 (en) 2011-12-09 2014-05-29 Butamax Advanced Biofuels Llc Process to remove product alcohols from fermentation broth by multistep flash evaporation
US9909148B2 (en) 2011-12-30 2018-03-06 Butamax Advanced Biofuels Llc Fermentative production of alcohols
US9181566B2 (en) 2011-12-30 2015-11-10 Butamax Advanced Biofuels Llc Genetic switches for butanol production
KR20140146616A (ko) 2012-03-23 2014-12-26 부타맥스 어드밴스드 바이오퓨얼스 엘엘씨 부타놀로겐용 배지의 아세테이트 보충물
MX2014013446A (es) * 2012-05-11 2014-12-08 Butamax Advanced Biofuels Llc Enzimas de cetol-acido reductoisomerasa y metodos de uso.
BR112015003701A2 (pt) 2012-08-22 2017-12-12 Butamax Advanced Biofuels Llc células hospedeiras recombinantes, método para aprimoramento, processo para a produção de um álcool, polinucleotídeo isolado, cassete de expressão e composição
WO2014047421A1 (en) 2012-09-21 2014-03-27 Butamax(Tm) Advanced Biofuels Llc Production of renewable hydrocarbon compositions
AU2013323427A1 (en) 2012-09-26 2015-03-05 Butamax (Tm) Advanced Biofuels Llc Polypeptides with ketol-acid reductoisomerase activity
US9273330B2 (en) 2012-10-03 2016-03-01 Butamax Advanced Biofuels Llc Butanol tolerance in microorganisms
WO2014106107A2 (en) 2012-12-28 2014-07-03 Butamax (Tm) Advanced Biofuels Llc Dhad variants for butanol production
US9034631B2 (en) * 2013-03-14 2015-05-19 Poet Research, Inc. Systems and methods for yeast propagation
ES2735024T3 (es) 2013-03-14 2019-12-13 Du Pont Glicerol-3-fosfato deshidrogenasa para la producción de butanol
US9580705B2 (en) 2013-03-15 2017-02-28 Butamax Advanced Biofuels Llc DHAD variants and methods of screening
WO2014144210A2 (en) 2013-03-15 2014-09-18 Butamax Advanced Biofuels Llc Competitive growth and/or production advantage for butanologen microorganism
WO2015002913A1 (en) * 2013-07-03 2015-01-08 Butamax Advanced Biofuels Llc Partial adaptation for butanol production
CA2957920A1 (en) 2014-08-11 2016-02-18 Butamax Advanced Biofuels Llc Yeast preparations and methods of making the same
RU2017128466A (ru) * 2015-02-10 2019-03-12 Эрбсле Гайзенхайм Аг Способ получения дрожжей-мутантов и их применение
CA3021033A1 (en) 2016-05-05 2017-11-09 Newpek S.A. De C.V. Enzymatic methods for butanol production

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2009055072A1 (en) * 2007-10-26 2009-04-30 Arbor Fuel Inc. Methods for the production of n-butanol
WO2009149270A2 (en) * 2008-06-04 2009-12-10 E. I. Du Pont De Nemours And Company A method for producing butanol using two-phase extractive fermentation

Family Cites Families (20)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4284724A (en) 1977-08-25 1981-08-18 Hideki Fukuda Method of highly concentrated cultivation of yeasts
US4414329A (en) 1980-01-15 1983-11-08 Phillips Petroleum Company Biochemical conversions by yeast fermentation at high cell densities
US4765992A (en) 1984-06-01 1988-08-23 Universite De Bordeaux Ii Stimulation of alcoholic fermentation by adsorption of toxic substances with cell walls
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US9297028B2 (en) 2005-09-29 2016-03-29 Butamax Advanced Biofuels Llc Fermentive production of four carbon alcohols
EP1948814B1 (en) * 2005-10-26 2018-11-21 Butamax (TM) Advanced Biofuels LLC Fermentive production of four carbon alcohols
US8945899B2 (en) 2007-12-20 2015-02-03 Butamax Advanced Biofuels Llc Ketol-acid reductoisomerase using NADH
US8956850B2 (en) 2008-06-05 2015-02-17 Butamax Advanced Biofuels Llc Enhanced pyruvate to acetolactate conversion in yeast
US8962298B2 (en) 2006-05-02 2015-02-24 Butamax Advanced Biofuels Llc Recombinant host cell comprising a diol dehydratase
US8828704B2 (en) 2006-05-02 2014-09-09 Butamax Advanced Biofuels Llc Fermentive production of four carbon alcohols
NZ579780A (en) 2007-04-18 2012-07-27 Butamax Advanced Biofuels Llc Fermentive production of isobutanol using highly active ketol-acid reductoisomerase enzymes
JP2010539988A (ja) 2007-10-04 2010-12-24 ビオ アルチテクトウレ ラブ インコーポレイテッド 生物燃料製品
ES2575413T3 (es) 2007-12-20 2016-06-28 Butamax (Tm) Advanced Biofuels Llc Cetol-ácido reductoisomerasa que utiliza NADH
CN101959568A (zh) 2007-12-27 2011-01-26 格沃股份有限公司 从稀水溶液回收高级醇
US8188250B2 (en) 2008-04-28 2012-05-29 Butamax(Tm) Advanced Biofuels Llc Butanol dehydrogenase enzyme from the bacterium Achromobacter xylosoxidans
CA2735690A1 (en) 2008-09-29 2010-04-01 Dennis Flint Identification and use of bacterial [2fe-2s] dihydroxy-acid dehydratases
WO2010062597A1 (en) 2008-10-27 2010-06-03 Butamax™ Advanced Biofuels LLC Carbon pathway optimized production hosts for the production of isobutanol
EP2446045A4 (en) 2009-06-26 2017-04-19 GEVO, Inc. Recovery of higher alcohols from dilute aqueous solutions
BR112012006939A2 (pt) 2009-09-29 2015-09-08 Butamax Tm Advanced Biofuels célula hospedeira de produção de levedura recombinante e método para a produção de um produto selecionado do grupo que consiste em 2,3-butanodiol, isobutanol, 2-butanol, 1-butanol, 2-butanona, valina, leucina, ácido lático, ácido málico, álcool, isoampilico e isoprenoides
CA2775892A1 (en) 2009-09-29 2011-04-07 Butamax(Tm) Advanced Biofuels Llc Fermentive production of isobutanol using highly effective ketol-acid reductoisomerase enzymes

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2009055072A1 (en) * 2007-10-26 2009-04-30 Arbor Fuel Inc. Methods for the production of n-butanol
WO2009149270A2 (en) * 2008-06-04 2009-12-10 E. I. Du Pont De Nemours And Company A method for producing butanol using two-phase extractive fermentation

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
B. SONNLEITNER ET AL: "Dynamics of Glucose Consumption in Yeast", 《BIOTECHNOLOGY PROGRESS》 *
ERIC P. KNOSHAUG ET AL: "Butanol Tolerance in a Selection of Microorganisms", 《APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY》 *
H. VAN URK ET AL: "Glucose Transport in Crabtree-positive and Crabtree-negative Yeasts", 《MICROBIOLOGY》 *
STEEN ERIC J ET AL: "Metabolic engineering of Saccharomyces cerevisiae for the production of n-butanol", 《MICROBIAL CELL FACTORIES》 *

Also Published As

Publication number Publication date
ZA201208575B (en) 2014-01-29
AU2011268326A1 (en) 2012-12-06
MX2012014550A (es) 2013-01-29
NZ603546A (en) 2014-10-31
CA2801576A1 (en) 2011-12-22
WO2011159894A1 (en) 2011-12-22
JP2013528398A (ja) 2013-07-11
US20120149080A1 (en) 2012-06-14
AU2011268326B2 (en) 2015-02-05
EP2582786A1 (en) 2013-04-24
KR20130027551A (ko) 2013-03-15
BR112012031847A2 (pt) 2016-02-16

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