CN103190364B - Method and injection liquid for nursing wild coreius guichenoti - Google Patents

Method and injection liquid for nursing wild coreius guichenoti Download PDF

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Publication number
CN103190364B
CN103190364B CN201310105747.9A CN201310105747A CN103190364B CN 103190364 B CN103190364 B CN 103190364B CN 201310105747 A CN201310105747 A CN 201310105747A CN 103190364 B CN103190364 B CN 103190364B
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China
Prior art keywords
fish
guichenoti
nursing
injection liquid
wild
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Expired - Fee Related
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CN201310105747.9A
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Chinese (zh)
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CN103190364A (en
Inventor
朱永久
杨德国
何勇凤
吴兴兵
高宇鹏
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Yangtze River Fisheries Research Institute CAFS
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Yangtze River Fisheries Research Institute CAFS
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    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02ATECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/80Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
    • Y02A40/81Aquaculture, e.g. of fish

Abstract

The invention discloses a method for nursing wild coreius guichenoti. Nursing injection liquid is injected into fish according to the fish size, then water is packaged in a plastic bag and oxygenated, and the fish is transported to a culture place. Before the fish is placed into a pond, a 3% NaCl solution is used for soaking the fish for 10 minutes, erythromycin ointment is used for coating wounded portions, after the fish is placed into the pond, nursing injection liquid is injected to the fish one by one every other day twice, and culture management is conducted. The nursing injection liquid is mainly composed of antibiotics, anti-stress medicine and energy mixture. By means of corresponding medicine treatment, stress reaction of the coreius guichenoti is reduced, simultaneously inflammation of the fish body is diminished, exogenous nutrient substances are supplemented appropriately, and wild coreius guichenoti damage caused by fishing and transportation is recovered as soon as possible. Culture survival rate of the collected coreius guichenoti under the artificial condition is increased, enough population for researching domestication and artificial reproduction technology of the coreius guichenoti is provided, mass production of the coreius guichenoti and artificial breeding and releasing become possible, and a purpose of protecting the coreius guichenoti resource is achieved.

Description

A kind of wild C. guichenoti care method and nursing injection liquid used
Technical field
The invention belongs to C. guichenoti cultural technique field, particularly relate to a kind of wild C. guichenoti care method and nursing injection liquid used.
Background technology
C. guichenoti is subordinate to Cyprinidae, Minnow subfamily, copper fish belongs to, and belongs to river course migration fishes.Liking pipelining life, omnivorousness, is the Main Commercial Fishes of Upper Yangtze River river section.Along with the progressively enforcement of Jinsha jiang River middle and lower reaches, Yalongjiang River master stream and main stream of the Yangtze hydroelectric development, the spawning ground of C. guichenoti and migration passage will be subject to destruction in various degree, and the existence of its species is faced with huge threat.Carrying out that C. guichenoti artificial fecundation releases is reduce the effective way of its resource failure, but not yet breaks through due to C. guichenoti artificial propagation techniques, and its artificial fecundation work of releasing at present there is no method and implements.Domestic multiple research unit is just carrying out raising and train of C. guichenoti and is studying with artificial propagation techniques, it is studied with fish mainly purchased from Upper Yangtze River fisherman catches, fisherman fishes for method many employings set gill net or the drift net of C. guichenoti, these are fished for instrument and injure larger to C. guichenoti, add that again C. guichenoti self is to the strong physilogical characteristics of external disturbance stress reaction, after causing it to buy back, only pass through conventional method process, store up survival rate extremely low, especially sexual maturity individuality almost none survival, obstacle is caused to carrying out the research of C. guichenoti artificial propagation techniques further.Find a kind of suitable wild C. guichenoti care method, that improves field collection C. guichenoti stores up survival rate, is the key of carrying out subsequent serial research.
Purchase injured lighter wild C. guichenoti from fisherman's ship, after transporting plant back, namely use the NaCl solution of 3% or the KMn0 of 20mg/L concentration 4soak 10-15 minute, after erythromycin is smeared to injury, put into holding pond and store up.
Prior art only applies conventional cultivation fish as black carp, grass carp, immersion anti-inflammatory method after the move operation such as silver carp and flathead, do not take according to the strong feature of C. guichenoti stress reaction to slow down accordingly stress measure, after making it transfer to artificial holding pond ambient condition from wild environment state, under long term is in the state of high stress, monitor body physiological biochemical indicator and can not return to normal value, show as slowly travelling, do not ingest, wound is difficult to recover even to worsen, finally because physique declines or secondary infection other diseases, cause death, batch survival rate is below 5%.
Summary of the invention
The object of the present invention is to provide a kind of wild C. guichenoti care method and nursing injection liquid used, be intended to solve prior art and only apply conventional cultivation fish as the immersion anti-inflammatory method after the move operations such as black carp, grass carp, silver carp and flathead, do not take according to the strong feature of C. guichenoti stress reaction to slow down accordingly stress the problem of measure.
The present invention is achieved in that a kind of wild C. guichenoti care method, and described wild C. guichenoti care method comprises the following steps:
The C. guichenoti collected, first according to fish body size injection nursing injection liquid, then be transported to the oxygenation of plastic bag water and store up place, before lower pond, NaCl solution with 3% soaks 10 minutes, smears injury erythromycin ointment, behind lower pond, every other day penetrate a nursing injection liquid by endnote, continuous 2 times;
Carry out storing up management, concrete grammar is: (1) keeps pond water quality norm controlling in following scope: water temperature 12 DEG C-25 DEG C, dissolved oxygen > 5mg/L, pH6.5-8.0, ammonia nitrogen < 0.5mg/L, cultured water < 0.1mg/L; (2) make regular check on the condition of the injury situation of change of fish body active state and injury, find that dead fish is pulled out in time; (3) can feed of throwing something and feeding be started after storing up 1 week, after fish normally eats food, proceed to other cultivating systems raise and train;
Described nursing injection liquid making method is: nursing injection liquid forms primarily of antibiotic, anti-stress medicine and energy mistura; It specifically consists of: Enrofloxacin 50mg, butafosfan 500mg, VB 120.5mg, V c200mg, ATP10mg, the NaCl with 0.7% is made into 20mL solution, and press the dosage intrathecal of 2mL/kg fish during use, liquid is now with the current.
Further, described wild C. guichenoti care method also comprises the preparation of holding pond further:
Holding pond is the nursing pond before wild C. guichenoti is raised and train, and requires into draining quick, easy to operate, makes, the general 5-10 square metre of single area, dark 1.2 meters of pond, the depth of water 1 meter with concrete, PP plate or fiberglass, is equipped with aerator equipment or circular water treating system.Before using, soak 2 hours with the liquor potassic permanganate of 20mg/L.
Further, described wild C. guichenoti care method also comprises the collection of wild C. guichenoti further: on the riverside harbour close to dredger or landing stage, arranging bleeding point, the transporting equipments such as preparing plastic bag, oxygen, propose to fish for requirement to fisherman, namely require that fisherman is in fishing operation process, action is light and slow, fast careful, alleviate the degree of injury to C. guichenoti, the C. guichenoti after catching transports bleeding point to as early as possible.
Further, the Enrofloxacin in described nursing injection formula of liquid can use gentamicin sulphate, penicillin to substitute.
The present invention adopts the care method injected in immersion in vitro and body and combine, effectively can suppress body surface injury bacteriological infection, alleviate the muscular fatigue that C. guichenoti produces because fishing for antagonism, alleviate stress reaction, ahead of time adapt to artificial environment and initial feeding time, thus make C. guichenoti store up survival rate to bring up to more than 60%.
Accompanying drawing explanation
Fig. 1 is the flow chart of wild C. guichenoti care method provided by the invention.
Embodiment
In order to make object of the present invention, technical scheme and advantage clearly understand, below in conjunction with drawings and Examples, the present invention is further elaborated.Should be appreciated that specific embodiment described herein only in order to explain the present invention, be not intended to limit the present invention.
Fig. 1 shows the realization flow of wild C. guichenoti care method provided by the invention.Concrete steps are as follows:
S101: the preparation of holding pond: holding pond is the nursing pond before wild C. guichenoti is raised and train, requires into draining quick, easy to operate, make with concrete, PP plate or fiberglass, the general 5-10 square metre of single area, dark 1.2 meters of pond, the depth of water 1 meter, is equipped with aerator equipment or circular water treating system.Before using, soak 2 hours with the liquor potassic permanganate of 20mg/L.
S102: the collection of wild C. guichenoti: bleeding point is being set on the riverside harbour close to dredger or landing stage, the transporting equipments such as preparing plastic bag, oxygen, propose to fish for requirement to fisherman, namely require that fisherman is in fishing operation process, action is light and slow, fast careful, alleviate the degree of injury to C. guichenoti, the C. guichenoti after catching transports bleeding point to as early as possible.
S103: care method: the C. guichenoti of collection, first according to fish body size injection nursing injection liquid, then be transported to the oxygenation of plastic bag water and store up place, before lower pond, NaCl solution with 3% soaks 10 minutes, smears injury erythromycin ointment, behind lower pond, every other day penetrate a nursing injection liquid by endnote, continuous 2 times.
Nursing injection liquid is prepared: nursing injection liquid forms primarily of antibiotic, anti-stress medicine and energy mistura.It specifically consists of: Enrofloxacin 50mg, butafosfan 500mg, VB 120.5mg, V c200mg, ATP10mg, the NaCl with 0.7% is made into 20mL solution, and press the dosage intrathecal of 2mL/kg fish during use, liquid is now with the current.
Store up management: (1) keeps pond water quality norm controlling in following scope: water temperature 12 DEG C-25 DEG C, dissolved oxygen > 5mg/L, pH6.5-8.0, ammonia nitrogen < 0.5mg/L, cultured water < 0.1mg/L.(2) make regular check on the condition of the injury situation of change of fish body active state and injury, find that dead fish is pulled out in time.(3) can feed of throwing something and feeding be started after storing up 1 week, after fish normally eats food, proceed to other cultivating systems raise and train.
The foregoing is only preferred embodiment of the present invention, not in order to limit the present invention, all any amendments done within the spirit and principles in the present invention, equivalent replacement and improvement etc., all should be included within protection scope of the present invention.

Claims (2)

1. a compound method for wild C. guichenoti nursing nursing injection liquid, it is characterized in that, the compound method of described nursing injection liquid is: described nursing injection liquid forms primarily of antibiotic, anti-stress medicine and energy mistura; It specifically consists of: Enrofloxacin 50mg, butafosfan 500mg, VB 120.5mg, VC200mg, ATP10mg, the NaCl with 0.7% is made into 20mL solution, and press the dosage intrathecal of 2mL/kg fish during use, liquid is now with the current.
2. the compound method of wild C. guichenoti nursing nursing injection liquid as claimed in claim 1, is characterized in that, the Enrofloxacin in described nursing injection formula of liquid can use gentamicin sulphate, penicillin to substitute.
CN201310105747.9A 2013-03-29 2013-03-29 Method and injection liquid for nursing wild coreius guichenoti Expired - Fee Related CN103190364B (en)

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CN107624683B (en) * 2017-09-21 2019-10-08 水利部中国科学院水工程生态研究所 It is a kind of to improve wild C. guichenoti and long Qi Wen Minnow raises and train the effective ways of survival rate
CN108064775B (en) * 2018-02-11 2021-08-06 水利部中国科学院水工程生态研究所 Artificial propagation method for cupfish with round mouth
CN109349170B (en) * 2018-11-15 2021-08-31 宜昌三江渔业有限公司 Artificial breeding method for cupfish with round mouth
CN109601435B (en) * 2019-01-18 2021-05-11 西藏自治区农牧科学院水产科学研究所 Domestication method of wild adult glyptosternum maculatum
CN110651740A (en) * 2019-09-17 2020-01-07 孙治忠 Anti-flow-rate ecological conservation (cultivation) technology

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CN101496812B (en) * 2008-01-28 2010-10-13 中国水产科学研究院东海水产研究所 Compositional agent for rapid healing large-sized Acipenser sinensis with serious injury
CN102178080B (en) * 2011-04-12 2012-11-21 吉林农业大学 Feeding method capable of promoting growth and increasing immunity and stress resistance of Cyprinus carpio var. jian
CN102406091B (en) * 2011-12-02 2013-03-20 中国水产科学研究院淡水渔业研究中心 Composite preparation for improving fish body transportation stress capability as well as preparation method and application thereof

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