CN107624683B - It is a kind of to improve wild C. guichenoti and long Qi Wen Minnow raises and train the effective ways of survival rate - Google Patents
It is a kind of to improve wild C. guichenoti and long Qi Wen Minnow raises and train the effective ways of survival rate Download PDFInfo
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Abstract
Wild C. guichenoti is improved and long Qi Wen Minnow raises and train the transport and quarantine method of survival rate the invention discloses a kind of.Step is: 1) place of origin is quarantined: being impregnated quarantine using methylene blue and antibiotic, is changed water and dressing daily；2) transport for long-distance: transport adds antibiotic and MS-222 with water；3) raise and train quarantine: last stage quarantine medication is identical as Quarantine on production site, feeds biological feed and tames and dociles food.The stage uniformly mixes qinghaosu and naphthoquine phosphate in dough afterwards；4) daily management: observing fish body daily, detects water quality main indicator；5) disease prevention and cure: it is directed to surface wound, bacteriosis, saprolegniasis, ich, taeniasis, is targetedly prevented and treated.It is easy to implement the method, it is easy to operate, the high survival rate of long-distance transport and quarantine is effectively raised up to 90% or more, while successfully laying the foundation for raising and train for next stage, so that C. guichenoti and the long Qi Wen Minnow artificial propagation development that everything goes well with your work.
Description
Technical field
The invention belongs to aquaculture fields, and in particular to a kind of wild C. guichenoti of raising and long Qi Wen Minnow are raised and train and survived
The effective ways of rate, including the whole process to transporting and raising and train quarantined from place of capture quarantine more particularly to quarantine procedures
In the wound of C. guichenoti and long Qi Wen Minnow is restored and the Control Technology of the diseases such as bacterium, mould, small melonworm and tapeworm,
Artificial propagation suitable for C. guichenoti and long Qi Wen Minnow.
Background technique
C. guichenoti (Coreiusguichenoti) and long Qi Wen Minnow (Rhinogobioventralis) are subordinate to carp shape
Mesh (Cypriniformes), (Cyprinidae) , Minnow subfamily (Gotiongiae) is the spy in Upper Yangtze River and its tributary to Cyprinidae
There are fish.As the fish for producing Drifting egg, the entire history of life is completed in river.Due to a large amount of built or in the water built
Electrical engineering has blocked its migration route, causes strong influence to population colonization, and reproductive population quantity is fewer and fewer, individual
Specification is also smaller and smaller.In order to restore C. guichenoti and long Qi Wen Minnow wild resource, domestic more research units expand round mouth
The tackling key problem of the artificial propagation techniques of copper fish and long Qi Wen Minnow.In the process, the greatest problem faced be wild C. guichenoti and
Long Qi Wen Minnow to raise and train survival rate extremely low.To find out its cause, the mainly captured fish body condition of the injury is heavier, stress reaction is fierce, holds
Easily by pathogenic infections such as bacterium, mould, small melonworm and tapeworms, so that fish body matter declines rapidly, cause to quarantine, transport and raise and train
Mortality in the process.
Chinese aquatic science president's river produce research institute in application in 2013 " a kind of wild C. guichenoti care method and
Nursing injection liquid used " patent (Patent publication No CN103190364A).Although this method can improve wild C. guichenoti
Survival rate, still, without the following point during pointedly solving C. guichenoti quarantine, transporting and raise and train: 1. stress be anti-
Stress be strong, it is not suitable with the processing such as immersion or smearing of manual injection and irritation chemicals；2. wound mycotic infection；3. small melon
Worm is parasitic；4. enteron aisle tapeworm is parasitic.In addition, few reports for specially improving long Qi Wen Minnow from quarantine method and raising and train survival rate
Road.
Summary of the invention
Wild C. guichenoti is improved and long Qi Wen Minnow raises and train having for survival rate the purpose of the invention is to provide a kind of
Efficacious prescriptions method, it is easy to implement the method, it is easy to operate, effectively raise the high survival rate of long-distance transport and quarantine up to 90% or more,
It successfully lays the foundation for raising and train for next stage simultaneously, so that everything goes well with your work opens for C. guichenoti and long Qi Wen Minnow artificial propagation
Exhibition.
In order to achieve the above purpose, the present invention uses following technical measures:
It is a kind of to improve wild C. guichenoti and long Qi Wen Minnow raises and train the effective ways of survival rate, it the steps include:
1, the place of origin quarantine: using 2-3ppm methylene blue, penicillin and each ten thousand unit of 1000-2000 of streptomysin original powder/
m3Water body impregnates the fish body 2-5d just captured, changes water and dressing 1-3 times daily, it is ensured that water quality is pure and fresh.
2, it transports for long-distance: by the concentration dissolution penicillin and streptomysin original powder of step 1 in transport water body, in addition, dilution adds
Add 20-30ppm MS-222.
3, raise and train quarantine: quarantine 4 weeks, the first half stage continued to implement by the quarantine scheme of the place of origin, using the Asia 2-3ppm
Methyl blue, penicillin and each ten thousand units of 1000-2000/m of streptomysin original powder3Water body impregnates fish body；Rear half stage, in per kilogram face
1-2g qinghaosu and 20-40mg naphthoquine phosphate mixture are uniformly mixed in group's material.
4, from the 3d of quarantine, frost Tubifex or water earthworm or bread bait feeding: are fed by the 1% of fish body weight
The biological feeds such as worm (are purchased) from the market.Start after ingesting, it is more by 2-3 times of normal usage supplement fish use in dough feed
Dimension (the friendly bioengineering Co., Ltd of Chengdu three)；Meanwhile 4:3 mixed biologic bait is pressed in dough feed, and gradually decrease life
The feeding volume of object bait, feeds feed completely within 2 weeks.
5, water quality management: quarantine needs oxygenation and mechanical filter, must replace 1-3 times daily by ultraviolet-sterilization
The new water of reason replaces 30-100% tap water according to feeding volume and fish density every time.In addition, detection water quality items main indicator,
Such as 18-25 DEG C of water temperature, dissolved oxygen >=7mg/L, pH value 7.5-8.5, total ammonia nitrogen≤0.2mg/L, cultured water≤0.01mg/L.
6, daily management: quarantine observes C. guichenoti and long Qi Wen Minnow 1-2 times daily, and observation fish body surface wound is extensive
Whether multiple degree, body surface have white point and mycotic infection, respiratory rate and the solely trip phenomenon that whether peels off.
7, disease prevention and cure: 1. surface wound: process phase 2 weeks, the main lesser drug of irritation using antibiotic etc,
Penicillin and streptomysin is dual anti-plus methylene blue processing；2. bacteriosis: regarding the situation of fish, use above-mentioned dual anti-plus methylene
Indigo plant processing 1 week or so, or 1-2d: sodium chloride 30-100ppm, strong chlorine oil 0.2-0.3ppm, En Nuosha are handled using following mixture
Star 2-3ppm, povidone iodine 1-5ppm.Above-mentioned drug dissolves respectively in order, splashes to former drug uniform to water mixing
Afterwards, then lower one kind of splashing thoroughly changes water after impregnating 22-26h；3. saprolegniasis: 2-3ppm methylene blue adds 2 weeks left sides of dual anti-processing
The right side, while 2-3 DEG C of water temperature is improved, guarantee to change water dressing daily；4. ich: quarantine early period is inhibited using methylene blue, after
Phase uses qinghaosu and naphthoquine phosphate mixture；5. taeniasis: the albendazole being dissolved in physiological saline is injected in oral cavity, and dosage is
3-20mg/kg weight gavages 3-5 times 1 time a day.
It by the above method, quarantines before before transport and formally raising and train, improves C. guichenoti and long Qi Wen Minnow
Fish body constitution, and raising and train quarantine, is quarantined and disease prevention and cure by the way of mild, is reduced fish body irritability, is tieed up
Fish body immunity is held, improves and raises and train survival rate (up to 90% or more), without the C. guichenoti and long fin kiss by quarantine
Minnow constitution is weak, easy infection germ, and survival rate is 75% or so or even 60% or less.Therefore, this method is C. guichenoti and long fin
The success of Wen Minnow artificial propagation provides guarantee.
Compared with prior art, the present invention having the following advantages that and effect:
1, the present invention is after being collected into C. guichenoti and long Qi Wen Minnow, since purpose fish irritability is strong, collects in fishing
Constitution declines in the process, easily catches.Transport is former using 2ppm methylene blue, penicillin and streptomysin to before raising and train ground
Powder each 1000 or 20,000,000 units/m3Water body impregnates fish body 2-5d.By short-term quarantine, fish body surface wound and physical strength
Restored, improves transportation survival rate.
2, the present invention has carried out the quarantine in two stages to transport to the purpose fish for raising and train ground, early period 2-3ppm methylene
Blue, penicillin and each ten thousand units of 1000-2000/m of streptomysin original powder3Water body impregnates fish body, to body surface disinfection and sterilization, pre- bacteriological protection
The generation of property disease, saprolegniasis etc.；Later period uniformly mixes 1-2g qinghaosu and 20-40mg phosphoric acid naphthols in per kilogram dough
Quinoline mixture, the main outburst for preventing ich, compared with mercurous nitrate, malachite green, qinghaosu and naphthoquine phosphate low toxicity,
Low-residual, it is small to fish body injury, and can achieve the purpose that kill small melonworm polypide.
Based on above-mentioned advantage, the present invention is a kind of to improve wild C. guichenoti and long Qi Wen Minnow raises and train the effective ways of survival rate
Can improve fish body constitution, reduce stress reaction, maintain fish body immunity, improve transport and raise and train high survival rate up to 90% with
On, the success for C. guichenoti and long Qi Wen Minnow artificial propagation provides safeguard.
Specific embodiment
Embodiment 1:
A specific embodiment of the invention is specifically described below.
A kind of to improve wild C. guichenoti and long Qi Wen Minnow raises and train the effective ways of survival rate, its step are as follows:
1, the place of origin is quarantined: C. guichenoti and long Qi Wen Minnow irritability are strong, and fish body constitution declines after capture, easily infect
Disease, therefore, transport will quarantine to before raising and train ground.It is each using 2 or 3ppm methylene blue, penicillin and streptomysin original powder
1000 or 20,000,000 units/m3Water body impregnates the fish body 2 or 3 just captured or 4 or 5d, changes water dressing 1 or 2 or 3 times daily, really
It is pure and fresh to be retained matter.
2, transport for long-distance: wait quarantine, after fish body is stablized, transport is to raising and train ground.It transports green by the dissolution of above-mentioned concentration in water body
Mycin and streptomysin original powder, in addition, dilution addition 20 or 30ppm MS-222.
3, raise and train quarantine: C. guichenoti and long fin kiss Minnow are transported to after raising and train ground, Yao Jinhang quarantine in 4 weeks.The first half stage
Continue to implement by the quarantine scheme of the place of origin, using 2 or 3ppm methylene blue, penicillin and streptomysin original powder each 1000 or 2000
Ten thousand units/m3Water body impregnates fish body 2 or 3 or 4 or 5d, changes water dressing daily；Rear half stage is uniformly mixed in per kilogram dough
Close 1 or 2g qinghaosu and 20 or 30 or 40mg naphthoquine phosphate mixture.
4, it bait feeding: from the 3d of quarantine, feeds the frost Tubifex through disinfection by ultraviolet light or is sterilized through rinsing
The biological feeds such as the water earthworm or bread worm of (Ozone Water or 1 ‰ dioxygen water sterilizations 1 or 2 or 3min).Start after ingesting, in dough
Fish multidimensional is supplemented by 2 or 3 times of normal usages in feed (protein content 40%-45%)；4:3 is pressed in dough feed simultaneously
Mixed biologic bait, and the feeding volume of biological feed is gradually decreased, feed is fed completely in 2 weeks.
5, water quality management: quarantine needs oxygenation and mechanical filter, and replacement daily 1 or 2 or 3 time by ultraviolet-sterilization
The new water of reason, according to feeding volume and fish density, each replacement 30 or 50 or 80 or 100% tap water.Water quality items predominantly detect
Index is as follows: water temperature 18 or 20 or 23 or 25 DEG C, dissolved oxygen >=7mg/L, pH value 7.5 or 7.8 or 8 or 8.3 or 8.5, total ammonia nitrogen
≤ 0.2mg/L, cultured water≤0.01mg/L.
6, daily management: quarantine observes fish body 1 or 2 times daily, and main attention observes fish body surface wound and restores journey
Whether degree, body surface have white point and water mold, respiratory rate and the solely trip phenomenon that whether peels off.
7, disease prevention and cure: 1. surface wound: process phase is 2 weeks, mainly uses the lesser medicine of irritation of antibiotic etc
Object.Penicillin and streptomysin is dual anti-plus methylene blue processing；2. bacteriosis: regarding the situation of fish, use antibiotic plus methylene
Base indigo plant handle 1 week or so, or use following mixture processing 1 or 2d: sodium chloride 30 or 40 or 50 or 60 or 70 or 80 or 90 or
100ppm, strong chlorine oil 0.2 or 0.3ppm, Enrofloxacin 2 or 3ppm, povidone iodine 1 or 3 or 5ppm.Above-mentioned drug divides in order
It does not dissolve, is splashed after former drug sufficiently dilutes to water body, add a kind of lower drug after mixing, impregnate 22 or 24
Or after 26h, thoroughly more renew water；3. saprolegniasis: adding dual anti-processing 2 weeks or so with the methylene blue of 2 or 3ppm, while improving water
2 or 3 DEG C of temperature thoroughly changes wait warm water (90% or more) dressing daily；4. ich: aiming at prevention, quarantine early period is using sub-
Methyl blue inhibits, and the later period uses qinghaosu and naphthoquine phosphate mixture, handles 1 time every 1 or 2d, coprocessing 3 or 4 or 5 times；5. silk ribbon
Parasitosis: the albendazole that is dissolved in physiological saline is injected in oral cavity, and dosage is 3 or 5 or 8 or 11 or 14 or 17 or 20mg/kg weight,
1 time a day, it gavages 3 or 4 or 5 times.
By abovementioned technology, the fish body constitution of C. guichenoti and long Qi Wen Minnow is improved, its transport is improved and survives
Rate (95% or more)；Quarantine is raised and train, is quarantined by the way of mild and disease prevention and cure, fish body irritability is reduced, is tieed up
Fish body immunity is held, improves and raises and train survival rate (90% or more).Success for C. guichenoti and long Qi Wen Minnow artificial propagation mentions
It has supplied to ensure.
Claims (1)
- Wild C. guichenoti is improved and long Qi Wen Minnow raises and train the effective ways of survival rate 1. a kind of, the steps include:A, the place of origin is quarantined: using 2-3ppm methylene blue, penicillin and each ten thousand units of 1000-2000/m of streptomysin original powder3Water Body impregnates the fish body 2-5d just captured, changes water and dressing 1-3 times daily, it is ensured that water quality is pure and fresh；B, it transports for long-distance: by the concentration dissolution penicillin and streptomysin original powder of step (A) in transport water body, in addition, dilution addition 20-30ppm MS-222；C, raise and train quarantine: quarantine 4 weeks, the first half stage continued to implement by the quarantine of the place of origin, using 2-3ppm methylene blue, blueness Mycin and each ten thousand units of 1000-2000/m of streptomysin original powder3Water body impregnates fish body；Rear half stage, in per kilogram dough Even mixing 1-2g qinghaosu and 20-40mg naphthoquine phosphate mixture；D, bait feeding: from the 3d of quarantine, frost Tubifex or water earthworm or bread worm biological feed is fed, starts to take the photograph After food, fish multidimensional is supplemented by 2-3 times of normal usage in dough feed；The mixed biologic bait in dough feed, and taming and dociling The feeding volume of biological feed is gradually decreased during supporting；E, water quality management: quarantine oxygenation and mechanical filter, the replacement daily 1-3 times new water by ultraviolet-sterilization processing, root According to feeding volume and fish density, 30-100% is replaced every time, in addition, detection water quality indices, control 18-25 DEG C of water body water temperature, Dissolved oxygen >=7mg/L, pH value 7.5-8.5, total ammonia nitrogen≤0.2mg/L, cultured water≤0.01mg/L；F, daily management: quarantine observes C. guichenoti and long Qi Wen Minnow 1-2 times daily, and observation fish body surface wound restores journey Degree, body surface have white point and mycotic infection, respiratory rate and the solely trip phenomenon that peels off；G, disease prevention and cure: 1. surface wound: process phase 2 weeks, using penicillin and streptomysin is dual anti-plus methylene blue processing；2. thin Bacterium property disease: add methylene blue to handle 1 week using penicillin and streptomysin are dual anti-, or 1-2d: chlorination is handled using following mixture Sodium 30-100ppm, strong chlorine oil 0.2-0.3ppm, Enrofloxacin 2-3ppm, povidone iodine 1-5ppm, above-mentioned drug are distinguished in order Dissolution, after former drug splash to water mixing it is uniform after, then lower one kind of splashing changes water after impregnating 22-26h；3. water mold Disease: 2-3ppm methylene blue adds penicillin and the dual anti-processing of streptomysin 2 weeks, while improving 2-3 DEG C of water temperature, changes water dressing daily； 4. ich: quarantine early period is inhibited using methylene blue, and the later period uses qinghaosu and naphthoquine phosphate mixture；5. taeniasis: The albendazole being dissolved in physiological saline is injected in oral cavity, and dosage is that 3-20mg/kg weight gavages 3-5 times 1 time a day.
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| CN110720569A (en) * | 2018-07-16 | 2020-01-24 | 镇江金山湖农业发展有限公司 | Feed for breeding copper fishes |
| CN110720409A (en) * | 2018-07-16 | 2020-01-24 | 镇江金山湖农业发展有限公司 | Culture method of copper fishes |
| CN109601435B (en) * | 2019-01-18 | 2021-05-11 | 西藏自治区农牧科学院水产科学研究所 | Domestication method of wild adult glyptosternum maculatum |
| CN110710484A (en) * | 2019-09-24 | 2020-01-21 | 孙治忠 | Nondestructive proliferation and releasing rescue technology |
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| CN103190364B (en) * | 2013-03-29 | 2015-04-22 | 中国水产科学研究院长江水产研究所 | Method and injection liquid for nursing wild coreius guichenoti |
| CN105165688B (en) * | 2015-10-19 | 2017-07-21 | 武汉汇潜生物科技有限公司 | A kind of nearly red Culter of wild black tail domesticating method |
| CN105875458B (en) * | 2016-05-18 | 2018-12-21 | 中国长江三峡集团公司中华鲟研究所 | A kind of tame and docile food method of wild long Qi Wen Minnow |
| CN105994039B (en) * | 2016-05-26 | 2018-09-28 | 中国长江三峡集团公司中华鲟研究所 | A kind of intensive culture method improving long Qi Wen Minnow seed rearing survival rates |
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