CN103125369A - Breeding method for multi-resistance hibiscus cannabinus variety - Google Patents

Breeding method for multi-resistance hibiscus cannabinus variety Download PDF

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CN103125369A
CN103125369A CN201310087724XA CN201310087724A CN103125369A CN 103125369 A CN103125369 A CN 103125369A CN 201310087724X A CN201310087724X A CN 201310087724XA CN 201310087724 A CN201310087724 A CN 201310087724A CN 103125369 A CN103125369 A CN 103125369A
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disease
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hybridization
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CN103125369B (en
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陈幼玉
祁建民
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Fujian Agriculture and Forestry University
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Fujian Agriculture and Forestry University
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Abstract

The invention discloses a breeding method for a multi-resistance hibiscus cannabinus variety, belonging to the technical field of hibiscus cannabinus disease resistance breeding. The breeding method comprises the steps that a high-yield hibiscus cannabinus variety is taken as a recurrent parent, the recurrent parent is respectively hybridized with an anthracnose resistant source parent, a root knot nematode disease resistant source parent and a gray mould resistant source parent, firstly the three main hibiscus cannabinus disease genes are respectively imported into one high-yield hibiscus cannabinus variety by adopting a hybridization back-crossing method, then the three main hibiscus cannabinus disease genes are polymerized together by adopting a hybridization re-crossing method, and finally a hibiscus cannabinus variety which is resistant to three diseases at the same time is bred. The multi-resistance hibiscus cannabinus variety bred by adopting the breeding method is good in resistance and high in yield, application amount of a pesticide can be obviously reduced, and the yield is increased while environmental pollution can also be reduced. By adopting the breeding method disclosed by the invention, the three main hibiscus cannabinus disease genes resistant to anthracnose, root knot nematode disease and gray mould can be polymerized together, the hibiscus cannabinus variety which is resistant to the three diseases at the same time can be bred, and cost for pesticide application and labour each mu is reduced, investment of farmers is reduced, income of the farmers is increased, and environmental pollution is reduced.

Description

A kind of selection of how anti-Kenaf Cultivars
Technical field
The present invention is specifically related to a kind of selection of how anti-Kenaf Cultivars, belongs to bluish dogbane breeding for disease resistance technical field.
Background technology
Bluish dogbane is Malvaceae Hibiscus annual herb phloem fiber crop, plants more with China, Thailand, India, Bangladesh.China introduced a fine variety in nineteen twenty-eight.First of China's bluish dogbane cultivated area and the gross yield Jun Ju world, all there is plantation in the provinces such as Anhui, Henan, Guangdong, Guangxi, Fujian, Zhejiang, Shandong, Shanxi, Jiangsu, Hunan, Hubei, Jiangxi, Sichuan.Bluish dogbane is mainly used in textile and paper, and the bastose silvery white is glossy, and the moisture absorption aproll is fast, is suitable for weaving a gunnysack, burlap, hemp floor mat and rope, and the boon after peeling is used for firing active carbon and fibreboard made, and leaf can be made animal feeding-stuff.The bluish dogbane Major Diseases has anthracnose, root knot nematode disease, gray mold etc.Bluish dogbane anthracnose is that China bluish dogbane produces one of the most serious disease of upper harm, early 1950s should disease be very popular in fiber crops district, the whole nation, make China North China, northeast bluish dogbane be forced to stop planting because this disease causes harm, until before and after 1975, owing to having introduced disease-resistant variety and bluish dogbane anthracnose being taked a series of comprehensive preventive health measuress, make just that bluish dogbane area and output are basicly stable gets off.Although at present China's Kenaf Cultivars of producing upper plantation relatively improves a lot with kind in the past to the resistance of disease, resistance level is not high, and the generation of disease is still more serious, and the yield stability that China bluish dogbane is produced is still restraining factors.Abroad the area because of the plantation bluish dogbane is less, and bluish dogbane is only had some fragmentary researchs.At present, in production in order to prevent and treat the bluish dogbane disease, mostly adopt the way of pesticide control, not only increased peasant's input, also caused serious environmental pollution, control bluish dogbane disease the most effectively, the way of economy and environmental protection is the disease-resistant Kenaf Cultivars of seed selection, but the disease-resistant variety of bluish dogbane mostly is the monoclonal antibody kind both at home and abroad at present, only a certain disease is had resistance, lack the kind of the anti-multiple diseases of energy simultaneously, in production, due to illness the harmful loss that causes is very large.Method of the present invention adopts the hybridization method that backcrosses will resist the gene of 3 kinds of main bluish dogbane diseases to import respectively in same high yield Kenaf Cultivars, then is hybridized method by hybridization they are aggregated to together, breeds to resist simultaneously the Kenaf Cultivars of 3 kinds of diseases.
Summary of the invention
The object of the present invention is to provide a kind of selection of how anti-Kenaf Cultivars, the how anti-Kenaf Cultivars better resistance that the method is bred, output is high, can significantly reduce the amount of application of agricultural chemicals, also can reduce environmental pollution when increasing output.
For reaching above purpose, the present invention by the following technical solutions:
1) take the high yield Kenaf Cultivars as recurrent parent, with anthracnose resistant parent-A, root knot nematode disease resistant parent-B, gray mold resistant parent-C hybridization, get 3 hybridization F respectively 1Generation;
2) plantation hybridization F 1Generation, F 2In generation, is to 3 combination F 2The disease resistance in generation is carried out inoculated identification, and each combination only identifies and its corresponding resistant parent disease-resistant source filter out the close individual plant of high resistance and agronomy and yield traits and recurrent parent, and backcross with recurrent parent respectively, BC must backcross 1F 1Generation;
3) plant the BC that backcrosses 1F 1Generation, BC 1F 2Generation, BC 1F 2For each combination, its disease resistance is carried out inoculated identification, its corresponding resistant parent disease-resistant source is only identified in each combination, screening high resistance strain from each combination, and screening agronomy individual plant and the recurrent parent close with recurrent parent with yield traits continues to backcross from each high resistance strain, gets BC 2F 1In generation, repeat above step to the BC that backcrosses 5F 1Generation;
4) plantation BC 5F 1Generation, BC 5F 2In generation, screening at last obtains the Kenaf Cultivars that agronomy is similar to recurrent parent with yield traits, distinguish high resistance anthracnose, root knot nematode disease and gray mold, respectively with K A, K B, K CExpression;
5) make hybrid combination K A/ K B, plant its hybridization F 1Generation: F 1-AB, at F 1-ABMake hybridization F flowering stage 1-AB/ K C
6) plantation F 1-AB/ K CHybridization F 1Generation: F 1-ABCAnd F 2Generation: F 2-ABC, to F 2-ABCDisease resistance is carried out inoculated identification, identifies anthracnose, root knot nematode disease and 3 kinds of pathogenies of gray mold, selects 3 kinds of equal high resistances of pathogeny and the agronomy individual plant close with recurrent parent with yield traits, then selects namely to breed how anti-Kenaf Cultivars through 1-2 for pedigree.
Wherein take high yield Kenaf Cultivars good fortune red 951 or good fortune red 992 as recurrent parent, take blunt close 5,85-224 or 85-133 as the anthracnose resistant parent, take J-1-113 as the root knot nematode disease resistant parent, take good fortune red 952 as the gray mold resistant parent.
Beneficial effect of the present invention:
Employing the inventive method can will resist the gene pyramiding of anthracnose, root knot nematode disease and 3 kinds of bluish dogbane Major Diseases of gray mold to arrive together, breeds to resist simultaneously the Kenaf Cultivars of 3 kinds of diseases.It is shorter that the method is bred the time of how anti-Kenaf Cultivars, breeds the better resistance of how anti-Kenaf Cultivars, and output is high, can significantly reduce the amount of application of agricultural chemicals, every mu is reduced spraying pesticide and recruitment cost 80-100 unit, alleviates the peasant and drop into, increase peasant income, reduce environmental pollution.
Embodiment
Embodiment 1:
1) take high yield Kenaf Cultivars " good fortune red 951 " as recurrent parent, respectively with high resistance anthracnose kind-A (blunt close 5, document: Li Defang, the discovery of bluish dogbane to the approximate immune high resistance material of anthracnose. Acta Agronomica Sinica, 1995,21(l): 122-123), root-knot nematode resistant kind-B (J-1-113), botrytis resistant kind-C (good fortune red 952) hybridization, get 3 hybridization F 1In generation, is respectively with F 1-A, F 1-B, F 1-CExpression;
2) plantation F 1-A, F 1-B, F 1-CAnd F 2In generation, is to 3 combination F 2The disease resistance in generation is carried out inoculated identification, and each combination only identifies and its corresponding resistant parent disease-resistant source filter out the close individual plant of high resistance and agronomy and yield traits and recurrent parent and recurrent parent and backcross, and BC must backcross 1F 1Generation;
3) plant the BC that backcrosses 1F 1Generation, BC 1F 2Generation, BC 1F 2For each combination, its disease resistance is carried out inoculated identification, its corresponding resistant parent disease-resistant source is only identified in each combination, screening high resistance strain from each combination, and screening high resistance and agronomy individual plant and the recurrent parent close with recurrent parent with yield traits backcross from each high resistance strain, get BC 2F 1Generation;
4) repeat above step 3) to the BC that backcrosses 5F 1Generation, plantation BC 5F 1Generation, BC 5F 2In generation, screening at last obtains the Kenaf Cultivars that agronomy is similar to recurrent parent with yield traits, distinguish high resistance anthracnose, root knot nematode disease and gray mold, respectively with K A, K B, K CExpression;
5) make hybrid combination K A/ K B, plant its hybridization F 1Generation (F 1-AB), at F 1-ABMake hybridization F when blooming 1-AB/ K C
6) plantation F 1-AB/ K CHybridization F 1Generation (F 1-ABC), F 2Generation (F 2-ABC), to F 2-ABCDisease resistance is identified, identifies anthracnose, root knot nematode disease and 3 kinds of pathogenies of gray mold, selects 3 kinds of equal high resistances of pathogeny and the agronomy individual plant close with recurrent parent with yield traits, then selects namely to breed how anti-Kenaf Cultivars " how anti-good fortune red 951 " through 1-2 for pedigree." how anti-good fortune red 951 " be high resistance anthracnose, root knot nematode disease and 3 kinds of diseases of gray mold simultaneously, better resistance, output increases 8-10% than its parent " good fortune red 951 ", can significantly reduce the amount of application of agricultural chemicals, every mu is reduced by 90 yuan of spraying pesticide costs, reduce the peasant and drop into, increase peasant income, reduce environmental pollution.
Embodiment 2:
1) take high yield Kenaf Cultivars " good fortune red 992 " as recurrent parent, with high resistance anthracnose kind-A (85-224), root-knot nematode resistant kind-B (J-1-113), botrytis resistant kind-C (good fortune red 952) hybridization, get 3 hybridization F respectively 1In generation, is respectively with F 1-A, F 1-B, F 1-CExpression;
2) plantation F 1-A, F 1-B, F 1-CAnd F 2In generation, is to 3 combination F 2The disease resistance in generation is carried out inoculated identification, and each combination only identifies and its corresponding resistant parent disease-resistant source filter out the close individual plant of high resistance and agronomy and yield traits and recurrent parent and recurrent parent and backcross, and BC must backcross 1F 1Generation;
3) plant the BC that backcrosses 1F 1Generation, BC 1F 2Generation, BC 1F 2For each combination, its disease resistance is carried out inoculated identification, its corresponding resistant parent disease-resistant source is only identified in each combination, screening high resistance strain from each combination, and screening high resistance and agronomy individual plant and the recurrent parent close with recurrent parent with yield traits backcross from each high resistance strain, get BC 2F 1Generation;
4) repeat above step 3) to the BC that backcrosses 5F 1Generation, plantation BC 5F 1Generation, BC 5F 2In generation, screening at last obtains the Kenaf Cultivars that agronomy is similar to recurrent parent with yield traits, distinguish high resistance anthracnose, root knot nematode disease and gray mold, respectively with K A, K B, K CExpression;
5) make hybrid combination K A/ K B, plant its hybridization F 1Generation (F 1-AB), at F 1-ABMake hybridization F when blooming 1-AB/ K C
6) plantation F 1-AB/ K CHybridization F 1Generation (F 1-ABC), F 2Generation (F 2-ABC), to F 2-ABCDisease resistance is identified, identifies anthracnose, root knot nematode disease and 3 kinds of pathogenies of gray mold, selects 3 kinds of equal high resistances of pathogeny and the agronomy individual plant close with recurrent parent with yield traits, then selects namely to breed how anti-Kenaf Cultivars " how anti-good fortune red 992 " through 1-2 for pedigree." how anti-good fortune red 992 " be high resistance anthracnose, root knot nematode disease and 3 kinds of diseases of gray mold simultaneously, better resistance, output increases 6-8% than its parent " good fortune red 992 ", can significantly reduce the amount of application of agricultural chemicals, every mu is reduced by 100 yuan of spraying pesticide costs, reduce the peasant and drop into, increase peasant income, reduce environmental pollution.
The above is only preferred embodiment of the present invention, and all equalizations of doing according to the present patent application the scope of the claims change and modify, and all should belong to covering scope of the present invention.

Claims (2)

1. the selection of anti-Kenaf Cultivars more than a kind, it is characterized in that: described selection comprises the following steps:
1) take the high yield Kenaf Cultivars as recurrent parent, with anthracnose resistant parent-A, root knot nematode disease resistant parent-B, gray mold resistant parent-C hybridization, get 3 hybridization F respectively 1Generation;
2) plantation hybridization F 1Generation, F 2In generation, is to 3 combination F 2The disease resistance in generation is carried out inoculated identification, and each combination only identifies and its corresponding resistant parent disease-resistant source filter out the close individual plant of high resistance and agronomy and yield traits and recurrent parent, and backcross with recurrent parent respectively, BC must backcross 1F 1Generation;
3) plant the BC that backcrosses 1F 1Generation, BC 1F 2Generation, BC 1F 2For each combination, its disease resistance is carried out inoculated identification, its corresponding resistant parent disease-resistant source is only identified in each combination, screening high resistance strain from each combination, and screening agronomy individual plant and the recurrent parent close with recurrent parent with yield traits continues to backcross from each high resistance strain, gets BC 2F 1In generation, repeat above step to the BC that backcrosses 5F 1Generation;
4) plantation BC 5F 1Generation, BC 5F 2In generation, screening at last obtains the Kenaf Cultivars that agronomy is similar to recurrent parent with yield traits, distinguish high resistance anthracnose, root knot nematode disease and gray mold, respectively with K A, K B, K CExpression;
5) make hybrid combination K A/ K B, plant its hybridization F 1Generation: F 1-AB, at F 1-ABMake hybridization F flowering stage 1-AB/ K C
6) plantation F 1-AB/ K CHybridization F 1Generation: F 1-ABCAnd F 2Generation: F 2-ABC, to F 2-ABCDisease resistance is carried out inoculated identification, identifies anthracnose, root knot nematode disease and 3 kinds of pathogenies of gray mold, selects 3 kinds of equal high resistances of pathogeny and the agronomy individual plant close with recurrent parent with yield traits, then selects namely to breed how anti-Kenaf Cultivars through 1-2 for pedigree.
2. the selection of according to claim 1 how anti-Kenaf Cultivars, it is characterized in that: take high yield Kenaf Cultivars good fortune red 951 or good fortune red 992 as recurrent parent, take blunt close 5,85-224 or 85-133 as the anthracnose resistant parent, take J-1-113 as the root knot nematode disease resistant parent, take good fortune red 952 as the gray mold resistant parent.
CN201310087724.XA 2013-03-19 2013-03-19 Breeding method for multi-resistance hibiscus cannabinus variety Expired - Fee Related CN103125369B (en)

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Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104126498A (en) * 2014-07-14 2014-11-05 福建农林大学 Saline-tolerant pest-disease-resistant cotton variety breeding method
CN104303992A (en) * 2014-10-27 2015-01-28 广西壮族自治区农业科学院蔬菜研究所 Breeding method for powdery mildew highly-resistant bitter gourd
CN104620978A (en) * 2015-02-10 2015-05-20 福建农林大学 Breeding method of cytoplasmic male sterile line of quality disease-resistant photo periodical insensitive hibiscus cannabinus
CN107980616A (en) * 2017-12-28 2018-05-04 福建农林大学 A kind of selection of how anti-yellowing numb kind
CN108094191A (en) * 2017-12-28 2018-06-01 福建农林大学 A kind of selection of the disease-resistant saline-alkali tolerant Kenaf Cultivars of Day neutral
CN110150050A (en) * 2018-03-15 2019-08-23 中国农业科学院蔬菜花卉研究所 A kind of anti-root-knot nematode pumpkin rootstock and its selection and application

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Cited By (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104126498A (en) * 2014-07-14 2014-11-05 福建农林大学 Saline-tolerant pest-disease-resistant cotton variety breeding method
CN104126498B (en) * 2014-07-14 2016-04-06 福建农林大学 A kind of selection of Salt And Alkali Tolerance Resistant cotton variety
CN104303992A (en) * 2014-10-27 2015-01-28 广西壮族自治区农业科学院蔬菜研究所 Breeding method for powdery mildew highly-resistant bitter gourd
CN104303992B (en) * 2014-10-27 2016-09-28 广西壮族自治区农业科学院蔬菜研究所 A kind of breeding method of high mildew-resistance Fructus Momordicae charantiae
CN104620978A (en) * 2015-02-10 2015-05-20 福建农林大学 Breeding method of cytoplasmic male sterile line of quality disease-resistant photo periodical insensitive hibiscus cannabinus
CN107980616A (en) * 2017-12-28 2018-05-04 福建农林大学 A kind of selection of how anti-yellowing numb kind
CN108094191A (en) * 2017-12-28 2018-06-01 福建农林大学 A kind of selection of the disease-resistant saline-alkali tolerant Kenaf Cultivars of Day neutral
CN108094191B (en) * 2017-12-28 2021-03-26 福建农林大学 Breeding method of light insensitive, salt-alkali-tolerant and disease-resistant kenaf variety
CN110150050A (en) * 2018-03-15 2019-08-23 中国农业科学院蔬菜花卉研究所 A kind of anti-root-knot nematode pumpkin rootstock and its selection and application

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