CN104126498A - Saline-tolerant pest-disease-resistant cotton variety breeding method - Google Patents

Saline-tolerant pest-disease-resistant cotton variety breeding method Download PDF

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CN104126498A
CN104126498A CN201410332969.9A CN201410332969A CN104126498A CN 104126498 A CN104126498 A CN 104126498A CN 201410332969 A CN201410332969 A CN 201410332969A CN 104126498 A CN104126498 A CN 104126498A
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resistant
cotton
salt
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variety
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CN104126498B (en
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章清杞
李晓燕
陈幼玉
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Xinjiang Jin Hongxiang hi tech agricultural Limited by Share Ltd
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Fujian Agriculture and Forestry University
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Abstract

The invention discloses a breeding method of a saline-tolerant pest-disease-resistant cotton variety. The invention relates to the technical field of cotton stress-resistance disease-resistance breeding. According to the invention, a cotton high-yield variety Dangza 02-3 or Quanmian No.5 is adopted as a recurrent parent. A variety Chuan 52-128 with high fusarium wilt resistance, a variety Zhongzhi BD18 with high verticillium wilt resistance, a variety Chuankang 77 with morphological aphid resistance, a variety U82-1 with high cotton bollworm resistance, and a variety Shannong N02 with saline tolerance are adopted as resistance source parents. With synchronous backcrossing and convergent hybridization methods, 5 main cotton pest-disease resistance sources and stress-resistance genes are converged and introduced into the high-yield cotton variety with a same genetic background, such that the multi-resistance high-yield cotton novel variety is obtained. The cotton variety has high resistances against 4 pest diseases, and is tolerant to saline. Other agronomic and yield traits of the variety are highly consistent with those of the original recurrent parent. With the method, yield can be increased, and environmental pollution caused by pesticide application can be reduced.

Description

A kind of selection of Salt And Alkali Tolerance Resistant cotton variety
Technical field
The present invention is specifically related to a kind of selection of Salt And Alkali Tolerance Resistant cotton variety, belongs to cotton disease resistance breeding technical field.
Background technology
Cotton is the important economic crops of China, and China is one of main Chan Mian big country of the world.In the growing process of cotton; often can be subjected to causing harm of multiple damage by disease and insect; the main diseases insect pests such as cotton is withered, verticillium wilt and cotton bollworm, pink bollworm, cotton spider mites, cotten aphid are on the rise; general time, cotton can cause economic loss 5-15% because caused harm by disease worm; if do not prevented and not treated; can cause the loss of 30%-50%, spraying pesticide can have been controlled causing harm of cotton diseases and insect pests effectively, increases output.But long-term a large amount of agricultural chemicals that uses, has greatly destroyed the ecological balance, has polluted environment, has caused the rampant again of damage by disease and insect, has increased the difficulty of control.Facts have proved, cultivate and promote Resistant new cotton variety and be one of the most simple and effective approach of cotton diseases and insect pests of controlling.But at present the cotton variety of China mostly is single resistant variety, be the sustainable development that promotes Cotton in China, must value and strengthen cotton disease resistance worm and Salt And Alkali Tolerance breeding, create Salt And Alkali Tolerance, anti-multiple damage by disease and insect, there is the new cotton variety of multiple resistance.
summary of the invention
The object of the present invention is to provide a kind of selection of Salt And Alkali Tolerance Resistant cotton variety, thereby greatly improve the resistance of cotton variety.
The present invention taking cotton yield kind when No. 5, assorted 02-3 or cotton as recurrent parent (Fang Peng flies. the cotton variety that overall merit is authorized by Anhui Province for 2010. Jiangxi cotton, 2011, 33(5): 50-55), with high resistance to wilt good kind river 52-128(Xiao Song China. Cotton in China progress of breeding and thinking of development. Jiangxi cotton, 204, 26(6): 3-11), in high resisting verticillium kind, plant BD18 (blue family sample. cotton disease resistance worm breeding research summary. hubei agricultural science, 2000, 4:20-22), the anti-aphid kind of form river anti-77(Xiao Song China. Study advance in cotton zoophobous breading. Jiangxi cotton, 1999, 21 (4): 3-10), high resistance helicoverpa armigera kind U82-1(Xiao Song China. Study advance in cotton zoophobous breading. Jiangxi cotton, 1999, 21 (4): 3-10), N02(is novel disease-resistant for the mountain agriculture of Salt And Alkali Tolerance kind, Salt And Alkali Tolerance New cotton line mountain agriculture N02 cultivates successfully. plant industry guide, 2013, 05 phase) be resistant parent, adopt and synchronously backcross and hybridize polymerization by 5 kinds of anti-sources of main cotton diseases and insect pests, resistance gene pyramiding imports in the high-yield cotton kind of same genetic background, be bred as 4 kinds of damage by disease and insect of high resistance and Salt And Alkali Tolerance simultaneously, what other agronomy was consistent with former recurrent parent height with yield traits resists more, high-yield cotton new varieties, thereby greatly improve the resistance of cotton variety, when increasing output, also can reduce pollution.
For reaching above object, the present invention by the following technical solutions:
1), taking R kind high-yield cotton kind as recurrent parent, plant that BD18, the anti-aphid kind of C kind form river are anti-77 respectively with A kind high resistance to wilt good kind river 52-128, the high resisting verticillium kind of B kind, D kind high resistance helicoverpa armigera kind U82-1, E kind Salt And Alkali Tolerance cotton variety mountain agriculture N02 hybridize and to obtain 5 hybridization F 1;
2) 5 hybridization F of plantation step 1) gained 1in generation, selfing obtains F 2generation, plantation F 2generation, F 2each combination of generation is identified its Resistant or salt tolerant alkalescence, its corresponding resistant parent institute's Resistant kind or salt tolerant alkalescence is only identified in each combination, filter out individual plant and the recurrent parent R that high Resistant or high Salt And Alkali Tolerance and agronomy and yield traits are close with recurrent parent R and backcross, BC must backcross 1f 1generation;
3) plantation step 2) the gained BC that backcrosses 1f 1generation, the BC that selfing obtains 1f 2generation, plantation BC 1f 2generation, each combination is identified its Resistant or salt tolerant alkalescence, its corresponding resistant parent institute's Resistant kind or salt tolerant alkalescence is only identified in each combination, filter out individual plant and the recurrent parent R that high Resistant or high Salt And Alkali Tolerance and agronomy and yield traits are close with recurrent parent R and continue to backcross, BC must backcross 2f 1generation;
4) repeat above 2), 3) step is to the BC that backcrosses 5f 1generation, plantation BC 5f 1generation, BC 5f 2in generation, finally screening obtains the cotton near-isogenic line of agronomy, high resistance corresponding damage by disease and insect similar to recurrent parent R with yield traits or salt tolerant alkalescence, respectively with K a, K b, K c, K d, K e,represent;
5) make hybridization K a/ K b, K c/ K d, K a/ K e, plant its hybridization F 1generation, F 2in generation,, respectively with F 2-AB, F 2-CD, F 2-AErepresent F 2each combination of generation is identified its Resistant or salt tolerant alkalescence, each combination only identifies that its corresponding resistant parent resists 2 kinds of sick worm kinds or salt tolerant alkalescence, filter out 1 of the individual plant that high Resistant or high Salt And Alkali Tolerance and agronomy and yield traits are close with recurrent parent R, make hybridization F 2-AB/ F 2-CD, F 2-CD/ F 2-AE, obtain 2 hybridization F 1, respectively with F 1-ABCD, F 1-CDAErepresent;
6) plantation step 5) gained F 1-ABCD, F 1-CDAE, make hybridization F 1-ABCD/ F 1-CDAE, obtain F 1-ABCDE, plantation F 1-ABCDE, obtain F 2-ABCDE, to F 2-ABCDEresistant or salt tolerant alkalescence are identified, identify that all resistant parents resist 5 kinds of sick worm kinds and salt tolerant alkalescence, filter out individual plant 10-20 that high Resistant and high Salt And Alkali Tolerance and agronomy and yield traits are close with recurrent parent R;
7) plantation step 6) gained Salt And Alkali Tolerance Resistant cotton strain, filter out the strain that economical character is close with recurrent parent R and yield traits is good and be bred as Salt And Alkali Tolerance Resistant cotton variety, this Salt And Alkali Tolerance Resistant cotton variety is Salt And Alkali Tolerance, high resistance to wilt good, verticillium wilt, aphid and cotton bollworm simultaneously.
A selection for Salt And Alkali Tolerance Resistant cotton variety, the high-yield cotton kind (recurrent parent R) described in it is: when No. 5, assorted 02-3 or cotton.
Beneficial effect of the present invention:
Effect of the present invention is respectively controlling cotton Salt And Alkali Tolerance, high resistance to wilt good, verticillium wilt, the anti-source gene of aphid and cotton bollworm imports in same cotton yield kind by hybridization back cross breeding method, be bred as the near-isogenic line containing identical genetic background and different resistant genes, again by the polymerization of hybridization between two of near-isogenic line, 5 kinds of resistant genes are aggregated in same genetic background high-yield cotton kind, not only simultaneously Salt And Alkali Tolerance and 4 kinds of damage by disease and insect of high resistance of the Salt And Alkali Tolerance Resistant cotton variety being bred as, other agronomy is highly consistent with former excellent cotton variety parent with yield traits, still possesses high yield, high-quality characteristic.Because of Salt And Alkali Tolerance Resistant, every mu of Salt And Alkali Tolerance Resistant cotton variety can increase output 10-12%, reduces spraying pesticide cost 80-100 unit.This breeding method breeding time that also more traditional introductory technique one by one can be saved 3-4.
Embodiment
Embodiment 1:
1) work as assorted 02-3 as recurrent parent (R kind) taking high-yield cotton kind, respectively with high resistance to wilt good kind river 52-128(A kind), plant BD18(B kind in high resisting verticillium kind), the anti-77(C kind in the anti-aphid kind of form river), high resistance helicoverpa armigera kind U82-1(D kind) hybridization, Salt And Alkali Tolerance cotton variety mountain agriculture N02(E kind) 5 hybridization F 1;
2) 5 hybridization F of plantation step 1) gained 1in generation, selfing obtains F 2generation, plantation F 2generation, F 2each combination of generation is identified its Resistant or salt tolerant alkalescence, its corresponding resistant parent institute's Resistant kind or salt tolerant alkalescence is only identified in each combination, filter out individual plant and the recurrent parent R that high Resistant or high Salt And Alkali Tolerance and agronomy and yield traits are close with recurrent parent R and backcross, BC must backcross 1f 1generation;
3) plantation step 2) the gained BC that backcrosses 1f 1generation, the BC that selfing obtains 1f 2generation, plantation BC 1f 2generation, each combination is identified its Resistant or salt tolerant alkalescence, its corresponding resistant parent institute's Resistant kind or salt tolerant alkalescence is only identified in each combination, filter out individual plant and the recurrent parent R that high Resistant or high Salt And Alkali Tolerance and agronomy and yield traits are close with recurrent parent R and continue to backcross, BC must backcross 2f 1generation;
4) repeat above 2), 3) step is to the BC that backcrosses 5f 1generation, plantation BC 5f 1generation, BC 5f 2in generation, finally screening obtains the cotton near-isogenic line of agronomy, high resistance corresponding damage by disease and insect similar to recurrent parent R with yield traits or salt tolerant alkalescence, respectively with K a, K b, K c, K d, K e,represent;
5) make hybridization K a/ K b, K c/ K d, K a/ K e, plant its hybridization F 1generation, F 2in generation, is (respectively with F 2-AB, F 2-CD, F 2-AErepresent), F 2each combination of generation is identified its Resistant or salt tolerant alkalescence, each combination only identifies that its corresponding resistant parent resists 2 kinds of sick worm kinds or salt tolerant alkalescence, filter out 1 of the individual plant that high Resistant or high Salt And Alkali Tolerance and agronomy and yield traits are close with recurrent parent R, make hybridization F 2-AB/ F 2-CD, F 2-CD/ F 2-AE, obtain 2 hybridization F 1(respectively with F 1-ABCD, F 1-CDAErepresent);
6) plantation step 5) gained F 1-ABCD, F 1-CDAE, make hybridization F 1-ABCD/ F 1-CDAE, obtain F 1-ABCDE, plantation F 1-ABCDE, obtain F 2-ABCDE, to F 2-ABCDEresistant or salt tolerant alkalescence are identified, identify that all resistant parents resist 5 kinds of sick worm kinds and salt tolerant alkalescence, filter out individual plant 10-20 that high Resistant and high Salt And Alkali Tolerance and agronomy and yield traits are close with recurrent parent R;
7) plantation step 6) gained Salt And Alkali Tolerance Resistant cotton strain, filter out the strain that economical character is close with recurrent parent R and yield traits is good and be bred as Salt And Alkali Tolerance Resistant cotton variety " how anti-as assorted 02-3 ", " how anti-as assorted 02-3 " Salt And Alkali Tolerance, high resistance to wilt good, verticillium wilt, aphid and cotton bollworm simultaneously.
" how anti-as assorted 02-3 " agronomy is highly consistent as assorted 02-3 with former excellent cotton variety parent with yield traits, possesses high yield, high-quality characteristic.Because of Salt And Alkali Tolerance Resistant, " resist as assorted 02-3 " every mu comparable when assorted 02-3 increase output 10% more, reduces by 80 yuan of spraying pesticide costs.This breeding method breeding time that also more traditional introductory technique one by one can be saved 3-4.
Embodiment 2:
1) taking No. 5, high-yield cotton kind cotton as recurrent parent (R kind), respectively with high resistance to wilt good kind river 52-128(A kind), plant BD18(B kind in high resisting verticillium kind), the anti-77(C kind in the anti-aphid kind of form river), high resistance helicoverpa armigera kind U82-1(D kind) hybridization, Salt And Alkali Tolerance cotton variety mountain agriculture N02(E kind) 5 hybridization F 1;
2) 5 hybridization F of plantation step 1) gained 1in generation, selfing obtains F 2generation, plantation F 2generation, F 2each combination of generation is identified its Resistant or salt tolerant alkalescence, its corresponding resistant parent institute's Resistant kind or salt tolerant alkalescence is only identified in each combination, filter out individual plant and the recurrent parent R that high Resistant or high Salt And Alkali Tolerance and agronomy and yield traits are close with recurrent parent R and backcross, BC must backcross 1f 1generation;
3) plantation step 2) the gained BC that backcrosses 1f 1generation, the BC that selfing obtains 1f 2generation, plantation BC 1f 2generation, each combination is identified its Resistant or salt tolerant alkalescence, its corresponding resistant parent institute's Resistant kind or salt tolerant alkalescence is only identified in each combination, filter out individual plant and the recurrent parent R that high Resistant or high Salt And Alkali Tolerance and agronomy and yield traits are close with recurrent parent R and continue to backcross, BC must backcross 2f 1generation;
4) repeat above 2), 3) step is to the BC that backcrosses 5f 1generation, plantation BC 5f 1generation, BC 5f 2in generation, finally screening obtains the cotton near-isogenic line of agronomy, high resistance corresponding damage by disease and insect similar to recurrent parent R with yield traits or salt tolerant alkalescence, respectively with K a, K b, K c, K d, K e,represent;
5) make hybridization K a/ K b, K c/ K d, K a/ K e, plant its hybridization F 1generation, F 2in generation, is (respectively with F 2-AB, F 2-CD, F 2-AErepresent), F 2each combination of generation is identified its Resistant or salt tolerant alkalescence, each combination only identifies that its corresponding resistant parent resists 2 kinds of sick worm kinds or salt tolerant alkalescence, filter out 1 of the individual plant that high Resistant or high Salt And Alkali Tolerance and agronomy and yield traits are close with recurrent parent R, make hybridization F 2-AB/ F 2-CD, F 2-CD/ F 2-AE, obtain 2 hybridization F 1(respectively with F 1-ABCD, F 1-CDAErepresent);
6) plantation step 5) gained F 1-ABCD, F 1-CDAE, make hybridization F 1-ABCD/ F 1-CDAE, obtain F 1-ABCDE, plantation F 1-ABCDE, obtain F 2-ABCDE, to F 2-ABCDEresistant or salt tolerant alkalescence are identified, identify that all resistant parents resist 5 kinds of sick worm kinds and salt tolerant alkalescence, filter out individual plant 10-20 that high Resistant and high Salt And Alkali Tolerance and agronomy and yield traits are close with recurrent parent R;
7) plantation step 6) gained Salt And Alkali Tolerance Resistant cotton strain, filter out the strain that economical character is close with recurrent parent R and yield traits is good and be bred as Salt And Alkali Tolerance Resistant cotton variety " No. 5, how anti-cotton ", " No. 5, how anti-cotton " be Salt And Alkali Tolerance, high resistance to wilt good, verticillium wilt, aphid and cotton bollworm simultaneously.
" No. 5, how anti-cotton " not only simultaneously Salt And Alkali Tolerance and 4 kinds of damage by disease and insect of high resistance, No. 5, other agronomy and yield traits and former excellent cotton variety parent cotton are highly consistent, possess high yield, high-quality characteristic.Because of Salt And Alkali Tolerance Resistant, " No. 5, how anti-cotton " every mu can increase output 12%, reduces by 100 yuan of spraying pesticide costs.This breeding method breeding time that also more traditional introductory technique one by one can be saved 3-4.
The foregoing is only preferred embodiment of the present invention, all equalizations of doing according to the present patent application the scope of the claims change and modify, and all should belong to covering scope of the present invention.

Claims (2)

1. a selection for Salt And Alkali Tolerance Resistant cotton variety, is characterized in that described selection comprises the following steps:
1), taking R kind high-yield cotton kind as recurrent parent, plant that BD18, the anti-aphid kind of C kind form river are anti-77 respectively with A kind high resistance to wilt good kind river 52-128, the high resisting verticillium kind of B kind, D kind high resistance helicoverpa armigera kind U82-1, E kind Salt And Alkali Tolerance cotton variety mountain agriculture N02 hybridize and to obtain 5 hybridization F 1;
2) 5 hybridization F of plantation step 1) gained 1in generation, selfing obtains F 2generation, plantation F 2generation, F 2each combination of generation is identified its Resistant or salt tolerant alkalescence, its corresponding resistant parent institute's Resistant kind or salt tolerant alkalescence is only identified in each combination, filter out individual plant and the recurrent parent R that high Resistant or high Salt And Alkali Tolerance and agronomy and yield traits are close with recurrent parent R and backcross, BC must backcross 1f 1generation;
3) plantation step 2) the gained BC that backcrosses 1f 1generation, the BC that selfing obtains 1f 2generation, plantation BC 1f 2generation, each combination is identified its Resistant or salt tolerant alkalescence, its corresponding resistant parent institute's Resistant kind or salt tolerant alkalescence is only identified in each combination, filter out individual plant and the recurrent parent R that high Resistant or high Salt And Alkali Tolerance and agronomy and yield traits are close with recurrent parent R and continue to backcross, BC must backcross 2f 1generation;
4) repeat above 2), 3) step is to the BC that backcrosses 5f 1generation, plantation BC 5f 1generation, BC 5f 2in generation, finally screening obtains the cotton near-isogenic line of agronomy, high resistance corresponding damage by disease and insect similar to recurrent parent R with yield traits or salt tolerant alkalescence, respectively with K a, K b, K c, K d, K e,represent;
5) make hybridization K a/ K b, K c/ K d, K a/ K e, plant its hybridization F 1generation, F 2in generation,, respectively with F 2-AB, F 2-CD, F 2-AErepresent F 2each combination of generation is identified its Resistant or salt tolerant alkalescence, each combination only identifies that its corresponding resistant parent resists 2 kinds of sick worm kinds or salt tolerant alkalescence, filter out 1 of the individual plant that high Resistant or high Salt And Alkali Tolerance and agronomy and yield traits are close with recurrent parent R, make hybridization F 2-AB/ F 2-CD, F 2-CD/ F 2-AE, obtain 2 hybridization F 1, respectively with F 1-ABCD, F 1-CDAErepresent;
6) plantation step 5) gained F 1-ABCD, F 1-CDAE, make hybridization F 1-ABCD/ F 1-CDAE, obtain F 1-ABCDE, plantation F 1-ABCDE, obtain F 2-ABCDE, to F 2-ABCDEresistant or salt tolerant alkalescence are identified, identify that all resistant parents resist 5 kinds of sick worm kinds and salt tolerant alkalescence, filter out individual plant 10-20 that high Resistant and high Salt And Alkali Tolerance and agronomy and yield traits are close with recurrent parent R;
7) plantation step 6) gained Salt And Alkali Tolerance Resistant cotton strain, filter out the strain that economical character is close with recurrent parent R and yield traits is good and be bred as Salt And Alkali Tolerance Resistant cotton variety, this Salt And Alkali Tolerance Resistant cotton variety is Salt And Alkali Tolerance, high resistance to wilt good, verticillium wilt, aphid and cotton bollworm simultaneously.
2. the selection of Salt And Alkali Tolerance Resistant cotton variety according to claim 1, is characterized in that: described recurrent parent R is: when No. 5, assorted 02-3 or cotton.
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CN105724237A (en) * 2016-02-18 2016-07-06 中国农业科学院棉花研究所 Method for screening high-yield cotton hybrid through subtending leaves
CN105961182A (en) * 2016-05-04 2016-09-28 江苏省农业科学院宿迁农科所 Method for cultivating high-lint and high-quality cotton variety
CN107750938A (en) * 2017-08-29 2018-03-06 长葛市联发粮食专业合作社 A kind of method for breeding wheat
CN111758555A (en) * 2020-07-15 2020-10-13 塔里木大学 Breeding method of new disease-resistant cotton variety

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Cited By (6)

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Publication number Priority date Publication date Assignee Title
CN105230179A (en) * 2015-09-28 2016-01-13 河北省农林科学院旱作农业研究所 Multi-stress synchronous stress breeding method for cotton breeding
CN105230179B (en) * 2015-09-28 2019-07-02 河北省农林科学院旱作农业研究所 A kind of more adverse circumstances of breeding cotton are synchronous to coerce method of breeding
CN105724237A (en) * 2016-02-18 2016-07-06 中国农业科学院棉花研究所 Method for screening high-yield cotton hybrid through subtending leaves
CN105961182A (en) * 2016-05-04 2016-09-28 江苏省农业科学院宿迁农科所 Method for cultivating high-lint and high-quality cotton variety
CN107750938A (en) * 2017-08-29 2018-03-06 长葛市联发粮食专业合作社 A kind of method for breeding wheat
CN111758555A (en) * 2020-07-15 2020-10-13 塔里木大学 Breeding method of new disease-resistant cotton variety

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