summary of the invention
The object of the present invention is to provide a kind of selection of Salt And Alkali Tolerance Resistant cotton variety, thereby greatly improve the resistance of cotton variety.
The present invention taking cotton yield kind when No. 5, assorted 02-3 or cotton as recurrent parent (Fang Peng flies. the cotton variety that overall merit is authorized by Anhui Province for 2010. Jiangxi cotton, 2011, 33(5): 50-55), with high resistance to wilt good kind river 52-128(Xiao Song China. Cotton in China progress of breeding and thinking of development. Jiangxi cotton, 204, 26(6): 3-11), in high resisting verticillium kind, plant BD18 (blue family sample. cotton disease resistance worm breeding research summary. hubei agricultural science, 2000, 4:20-22), the anti-aphid kind of form river anti-77(Xiao Song China. Study advance in cotton zoophobous breading. Jiangxi cotton, 1999, 21 (4): 3-10), high resistance helicoverpa armigera kind U82-1(Xiao Song China. Study advance in cotton zoophobous breading. Jiangxi cotton, 1999, 21 (4): 3-10), N02(is novel disease-resistant for the mountain agriculture of Salt And Alkali Tolerance kind, Salt And Alkali Tolerance New cotton line mountain agriculture N02 cultivates successfully. plant industry guide, 2013, 05 phase) be resistant parent, adopt and synchronously backcross and hybridize polymerization by 5 kinds of anti-sources of main cotton diseases and insect pests, resistance gene pyramiding imports in the high-yield cotton kind of same genetic background, be bred as 4 kinds of damage by disease and insect of high resistance and Salt And Alkali Tolerance simultaneously, what other agronomy was consistent with former recurrent parent height with yield traits resists more, high-yield cotton new varieties, thereby greatly improve the resistance of cotton variety, when increasing output, also can reduce pollution.
For reaching above object, the present invention by the following technical solutions:
1), taking R kind high-yield cotton kind as recurrent parent, plant that BD18, the anti-aphid kind of C kind form river are anti-77 respectively with A kind high resistance to wilt good kind river 52-128, the high resisting verticillium kind of B kind, D kind high resistance helicoverpa armigera kind U82-1, E kind Salt And Alkali Tolerance cotton variety mountain agriculture N02 hybridize and to obtain 5 hybridization F
1;
2) 5 hybridization F of plantation step 1) gained
1in generation, selfing obtains F
2generation, plantation F
2generation, F
2each combination of generation is identified its Resistant or salt tolerant alkalescence, its corresponding resistant parent institute's Resistant kind or salt tolerant alkalescence is only identified in each combination, filter out individual plant and the recurrent parent R that high Resistant or high Salt And Alkali Tolerance and agronomy and yield traits are close with recurrent parent R and backcross, BC must backcross
1f
1generation;
3) plantation step 2) the gained BC that backcrosses
1f
1generation, the BC that selfing obtains
1f
2generation, plantation BC
1f
2generation, each combination is identified its Resistant or salt tolerant alkalescence, its corresponding resistant parent institute's Resistant kind or salt tolerant alkalescence is only identified in each combination, filter out individual plant and the recurrent parent R that high Resistant or high Salt And Alkali Tolerance and agronomy and yield traits are close with recurrent parent R and continue to backcross, BC must backcross
2f
1generation;
4) repeat above 2), 3) step is to the BC that backcrosses
5f
1generation, plantation BC
5f
1generation, BC
5f
2in generation, finally screening obtains the cotton near-isogenic line of agronomy, high resistance corresponding damage by disease and insect similar to recurrent parent R with yield traits or salt tolerant alkalescence, respectively with K
a, K
b, K
c, K
d, K
e,represent;
5) make hybridization K
a/ K
b, K
c/ K
d, K
a/ K
e, plant its hybridization F
1generation, F
2in generation,, respectively with F
2-AB, F
2-CD, F
2-AErepresent F
2each combination of generation is identified its Resistant or salt tolerant alkalescence, each combination only identifies that its corresponding resistant parent resists 2 kinds of sick worm kinds or salt tolerant alkalescence, filter out 1 of the individual plant that high Resistant or high Salt And Alkali Tolerance and agronomy and yield traits are close with recurrent parent R, make hybridization F
2-AB/ F
2-CD, F
2-CD/ F
2-AE, obtain 2 hybridization F
1, respectively with F
1-ABCD, F
1-CDAErepresent;
6) plantation step 5) gained F
1-ABCD, F
1-CDAE, make hybridization F
1-ABCD/ F
1-CDAE, obtain F
1-ABCDE, plantation F
1-ABCDE, obtain F
2-ABCDE, to F
2-ABCDEresistant or salt tolerant alkalescence are identified, identify that all resistant parents resist 5 kinds of sick worm kinds and salt tolerant alkalescence, filter out individual plant 10-20 that high Resistant and high Salt And Alkali Tolerance and agronomy and yield traits are close with recurrent parent R;
7) plantation step 6) gained Salt And Alkali Tolerance Resistant cotton strain, filter out the strain that economical character is close with recurrent parent R and yield traits is good and be bred as Salt And Alkali Tolerance Resistant cotton variety, this Salt And Alkali Tolerance Resistant cotton variety is Salt And Alkali Tolerance, high resistance to wilt good, verticillium wilt, aphid and cotton bollworm simultaneously.
A selection for Salt And Alkali Tolerance Resistant cotton variety, the high-yield cotton kind (recurrent parent R) described in it is: when No. 5, assorted 02-3 or cotton.
Beneficial effect of the present invention:
Effect of the present invention is respectively controlling cotton Salt And Alkali Tolerance, high resistance to wilt good, verticillium wilt, the anti-source gene of aphid and cotton bollworm imports in same cotton yield kind by hybridization back cross breeding method, be bred as the near-isogenic line containing identical genetic background and different resistant genes, again by the polymerization of hybridization between two of near-isogenic line, 5 kinds of resistant genes are aggregated in same genetic background high-yield cotton kind, not only simultaneously Salt And Alkali Tolerance and 4 kinds of damage by disease and insect of high resistance of the Salt And Alkali Tolerance Resistant cotton variety being bred as, other agronomy is highly consistent with former excellent cotton variety parent with yield traits, still possesses high yield, high-quality characteristic.Because of Salt And Alkali Tolerance Resistant, every mu of Salt And Alkali Tolerance Resistant cotton variety can increase output 10-12%, reduces spraying pesticide cost 80-100 unit.This breeding method breeding time that also more traditional introductory technique one by one can be saved 3-4.
Embodiment
Embodiment 1:
1) work as assorted 02-3 as recurrent parent (R kind) taking high-yield cotton kind, respectively with high resistance to wilt good kind river 52-128(A kind), plant BD18(B kind in high resisting verticillium kind), the anti-77(C kind in the anti-aphid kind of form river), high resistance helicoverpa armigera kind U82-1(D kind) hybridization, Salt And Alkali Tolerance cotton variety mountain agriculture N02(E kind) 5 hybridization F
1;
2) 5 hybridization F of plantation step 1) gained
1in generation, selfing obtains F
2generation, plantation F
2generation, F
2each combination of generation is identified its Resistant or salt tolerant alkalescence, its corresponding resistant parent institute's Resistant kind or salt tolerant alkalescence is only identified in each combination, filter out individual plant and the recurrent parent R that high Resistant or high Salt And Alkali Tolerance and agronomy and yield traits are close with recurrent parent R and backcross, BC must backcross
1f
1generation;
3) plantation step 2) the gained BC that backcrosses
1f
1generation, the BC that selfing obtains
1f
2generation, plantation BC
1f
2generation, each combination is identified its Resistant or salt tolerant alkalescence, its corresponding resistant parent institute's Resistant kind or salt tolerant alkalescence is only identified in each combination, filter out individual plant and the recurrent parent R that high Resistant or high Salt And Alkali Tolerance and agronomy and yield traits are close with recurrent parent R and continue to backcross, BC must backcross
2f
1generation;
4) repeat above 2), 3) step is to the BC that backcrosses
5f
1generation, plantation BC
5f
1generation, BC
5f
2in generation, finally screening obtains the cotton near-isogenic line of agronomy, high resistance corresponding damage by disease and insect similar to recurrent parent R with yield traits or salt tolerant alkalescence, respectively with K
a, K
b, K
c, K
d, K
e,represent;
5) make hybridization K
a/ K
b, K
c/ K
d, K
a/ K
e, plant its hybridization F
1generation, F
2in generation, is (respectively with F
2-AB, F
2-CD, F
2-AErepresent), F
2each combination of generation is identified its Resistant or salt tolerant alkalescence, each combination only identifies that its corresponding resistant parent resists 2 kinds of sick worm kinds or salt tolerant alkalescence, filter out 1 of the individual plant that high Resistant or high Salt And Alkali Tolerance and agronomy and yield traits are close with recurrent parent R, make hybridization F
2-AB/ F
2-CD, F
2-CD/ F
2-AE, obtain 2 hybridization F
1(respectively with F
1-ABCD, F
1-CDAErepresent);
6) plantation step 5) gained F
1-ABCD, F
1-CDAE, make hybridization F
1-ABCD/ F
1-CDAE, obtain F
1-ABCDE, plantation F
1-ABCDE, obtain F
2-ABCDE, to F
2-ABCDEresistant or salt tolerant alkalescence are identified, identify that all resistant parents resist 5 kinds of sick worm kinds and salt tolerant alkalescence, filter out individual plant 10-20 that high Resistant and high Salt And Alkali Tolerance and agronomy and yield traits are close with recurrent parent R;
7) plantation step 6) gained Salt And Alkali Tolerance Resistant cotton strain, filter out the strain that economical character is close with recurrent parent R and yield traits is good and be bred as Salt And Alkali Tolerance Resistant cotton variety " how anti-as assorted 02-3 ", " how anti-as assorted 02-3 " Salt And Alkali Tolerance, high resistance to wilt good, verticillium wilt, aphid and cotton bollworm simultaneously.
" how anti-as assorted 02-3 " agronomy is highly consistent as assorted 02-3 with former excellent cotton variety parent with yield traits, possesses high yield, high-quality characteristic.Because of Salt And Alkali Tolerance Resistant, " resist as assorted 02-3 " every mu comparable when assorted 02-3 increase output 10% more, reduces by 80 yuan of spraying pesticide costs.This breeding method breeding time that also more traditional introductory technique one by one can be saved 3-4.
Embodiment 2:
1) taking No. 5, high-yield cotton kind cotton as recurrent parent (R kind), respectively with high resistance to wilt good kind river 52-128(A kind), plant BD18(B kind in high resisting verticillium kind), the anti-77(C kind in the anti-aphid kind of form river), high resistance helicoverpa armigera kind U82-1(D kind) hybridization, Salt And Alkali Tolerance cotton variety mountain agriculture N02(E kind) 5 hybridization F
1;
2) 5 hybridization F of plantation step 1) gained
1in generation, selfing obtains F
2generation, plantation F
2generation, F
2each combination of generation is identified its Resistant or salt tolerant alkalescence, its corresponding resistant parent institute's Resistant kind or salt tolerant alkalescence is only identified in each combination, filter out individual plant and the recurrent parent R that high Resistant or high Salt And Alkali Tolerance and agronomy and yield traits are close with recurrent parent R and backcross, BC must backcross
1f
1generation;
3) plantation step 2) the gained BC that backcrosses
1f
1generation, the BC that selfing obtains
1f
2generation, plantation BC
1f
2generation, each combination is identified its Resistant or salt tolerant alkalescence, its corresponding resistant parent institute's Resistant kind or salt tolerant alkalescence is only identified in each combination, filter out individual plant and the recurrent parent R that high Resistant or high Salt And Alkali Tolerance and agronomy and yield traits are close with recurrent parent R and continue to backcross, BC must backcross
2f
1generation;
4) repeat above 2), 3) step is to the BC that backcrosses
5f
1generation, plantation BC
5f
1generation, BC
5f
2in generation, finally screening obtains the cotton near-isogenic line of agronomy, high resistance corresponding damage by disease and insect similar to recurrent parent R with yield traits or salt tolerant alkalescence, respectively with K
a, K
b, K
c, K
d, K
e,represent;
5) make hybridization K
a/ K
b, K
c/ K
d, K
a/ K
e, plant its hybridization F
1generation, F
2in generation, is (respectively with F
2-AB, F
2-CD, F
2-AErepresent), F
2each combination of generation is identified its Resistant or salt tolerant alkalescence, each combination only identifies that its corresponding resistant parent resists 2 kinds of sick worm kinds or salt tolerant alkalescence, filter out 1 of the individual plant that high Resistant or high Salt And Alkali Tolerance and agronomy and yield traits are close with recurrent parent R, make hybridization F
2-AB/ F
2-CD, F
2-CD/ F
2-AE, obtain 2 hybridization F
1(respectively with F
1-ABCD, F
1-CDAErepresent);
6) plantation step 5) gained F
1-ABCD, F
1-CDAE, make hybridization F
1-ABCD/ F
1-CDAE, obtain F
1-ABCDE, plantation F
1-ABCDE, obtain F
2-ABCDE, to F
2-ABCDEresistant or salt tolerant alkalescence are identified, identify that all resistant parents resist 5 kinds of sick worm kinds and salt tolerant alkalescence, filter out individual plant 10-20 that high Resistant and high Salt And Alkali Tolerance and agronomy and yield traits are close with recurrent parent R;
7) plantation step 6) gained Salt And Alkali Tolerance Resistant cotton strain, filter out the strain that economical character is close with recurrent parent R and yield traits is good and be bred as Salt And Alkali Tolerance Resistant cotton variety " No. 5, how anti-cotton ", " No. 5, how anti-cotton " be Salt And Alkali Tolerance, high resistance to wilt good, verticillium wilt, aphid and cotton bollworm simultaneously.
" No. 5, how anti-cotton " not only simultaneously Salt And Alkali Tolerance and 4 kinds of damage by disease and insect of high resistance, No. 5, other agronomy and yield traits and former excellent cotton variety parent cotton are highly consistent, possess high yield, high-quality characteristic.Because of Salt And Alkali Tolerance Resistant, " No. 5, how anti-cotton " every mu can increase output 12%, reduces by 100 yuan of spraying pesticide costs.This breeding method breeding time that also more traditional introductory technique one by one can be saved 3-4.
The foregoing is only preferred embodiment of the present invention, all equalizations of doing according to the present patent application the scope of the claims change and modify, and all should belong to covering scope of the present invention.