CN101558754A - Method for select-breeding anti-virosis Bombyx mori varieties by utilizing Thailand Bombyx mori disease-resistant gene - Google Patents
Method for select-breeding anti-virosis Bombyx mori varieties by utilizing Thailand Bombyx mori disease-resistant gene Download PDFInfo
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- 241000255789 Bombyx mori Species 0.000 title claims abstract description 55
- 201000010099 disease Diseases 0.000 title claims abstract description 37
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 title claims abstract description 37
- 238000000034 method Methods 0.000 title claims abstract description 26
- 108090000623 proteins and genes Proteins 0.000 title claims abstract description 18
- 238000009395 breeding Methods 0.000 title abstract description 7
- 208000036142 Viral infection Diseases 0.000 title abstract 2
- 241000700605 Viruses Species 0.000 claims abstract description 36
- 238000012216 screening Methods 0.000 claims abstract description 11
- 241000701366 unidentified nuclear polyhedrosis viruses Species 0.000 claims abstract description 8
- 230000002068 genetic effect Effects 0.000 claims abstract description 4
- 230000000306 recurrent effect Effects 0.000 claims abstract description 4
- 235000013305 food Nutrition 0.000 claims description 30
- 239000007788 liquid Substances 0.000 claims description 24
- 210000002845 virion Anatomy 0.000 claims description 24
- 239000002574 poison Substances 0.000 claims description 10
- 231100000614 poison Toxicity 0.000 claims description 10
- 238000009396 hybridization Methods 0.000 claims description 6
- 230000008774 maternal effect Effects 0.000 claims description 6
- 230000000840 anti-viral effect Effects 0.000 claims description 5
- 230000008676 import Effects 0.000 claims description 5
- 238000004519 manufacturing process Methods 0.000 abstract description 7
- 230000001488 breeding effect Effects 0.000 abstract description 4
- 208000035240 Disease Resistance Diseases 0.000 abstract description 3
- 230000002155 anti-virotic effect Effects 0.000 abstract 2
- 230000000384 rearing effect Effects 0.000 abstract 1
- 208000037972 tropical disease Diseases 0.000 abstract 1
- 235000008708 Morus alba Nutrition 0.000 description 2
- 240000000249 Morus alba Species 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 239000004744 fabric Substances 0.000 description 2
- 206010003694 Atrophy Diseases 0.000 description 1
- 241000409811 Bombyx mori nucleopolyhedrovirus Species 0.000 description 1
- KRHYYFGTRYWZRS-UHFFFAOYSA-M Fluoride anion Chemical compound [F-] KRHYYFGTRYWZRS-UHFFFAOYSA-M 0.000 description 1
- 208000033809 Suppuration Diseases 0.000 description 1
- 238000009825 accumulation Methods 0.000 description 1
- 230000037444 atrophy Effects 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 230000001086 cytosolic effect Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 230000002708 enhancing effect Effects 0.000 description 1
- 238000012252 genetic analysis Methods 0.000 description 1
- 238000011835 investigation Methods 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
- 238000009366 sericulture Methods 0.000 description 1
- 230000003612 virological effect Effects 0.000 description 1
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Abstract
The invention discloses a method for select-breeding anti-virosis Bombyx mori varieties by using Thailand Bombyx mori disease-resistant gene. The method comprises the steps of: selecting the present Bombyx mori varieties of 871 and 872 used both in spring and autumn in China as female parent and using the introduced Thailand tropical disease-resistant variety NL as male parent; respectively hybridizing and leading-in the disease-resistance gene; hybridizing second generation and third generation silkworm and respectively adopting mixed batches rearing for breeding; hybridizing each generation beginning from a second age and screening by adopting 'population feeding' NPV (nuclear polyhedrosis virus) and a method with the concentration of virus feeding 'increasing age by age in a gradient way'; taking the Bombyx mori varieties 871 and 872 as recurrent parent, respectively adopting reserve seed antivirus silkworm individual to backcross, using a method of the combination of 'backcrossing introduction' and 'generation directed virus feeding screening breeding' to obtain an NL pure and mild system with comparatively stable genetic character by a plurality of backcrosses and virus feeding screening. In the process of select-breeding, various economic characters and production properties of the system are consolidated and improved and the objects of pure breeds of 871NL and 872NL are bred. The obtained antivirus Bombyx mori variety has good economic characters, is sturdy and easy to keep.
Description
Technical field:
The present invention relates to a kind of domestic silkworm strain resistant to virus disease selection, belong to crops improved seeds culture utilization field.
Background technology:
World silk science and technology developed country is when first-elected Japan.Since nineteen ninety-eight, conventional silk production is no longer studied, but turned to basic biological study because the restriction of labour and land resources Japan silk is produced atrophy gradually.China is the place of origin of silk industry.Sericulture is the important component part in present China rural economy, the total kind of Sang Yangcan peasant household in the whole nation more than 2,000 ten thousand families, spread all over 20 surplus a provinces and regions.Silk cocoon and raw silk output account for Gross World Product 3/4ths, silk fabric output accounts for the world more than 2/3rds, the raw silk export volume accounts for the world 80%, the silk fabric export volume accounts for the world more than 60%, silk already is the pillar industry of the vast wealth accumulation in rural areas of China, also is the industry of giving priority to of the structural readjustment of rural industry.
The silkworm virus disease is the important diseases that silkworm and mulberry are produced.In recent years, China enlarges gradually because of the loss that silkworm blood type pus illness (BmNPV), silkworm mind-intesting cytoplasmic polyhedrosis (BmCPV), silkworm virus flacherie (BmDNV) cause, the incidence of disease even up to 90%, and the serious threat silkworm and mulberry are produced.Owing to lack effective treatment means, flacherie remains one of limiting factor that improves cocoon yield and quality in the silk cocoon production at present.It is reported that China's silk cocoon production is generally because of the flacherie underproduction 10%～20%, serious reaches 80%～90%, even No kernels or seeds are gathered, as in a year of scarcity.Particularly the direct economic loss that causes because of harm such as silkworm virus disease, pebrine, fluoride pollutions is nearly 1,000,000,000 yuan.Reached in silk output under the prerequisite of certain level, increase the resistance against diseases of silkworm, reduce the production loss in the production process, it is one of important channel of improving the Silk Industry productivity, and the cultivation disease-resistant variety is set up the diseases prevention pre-alarming system and is that the control flacherie breaks out is most economical, effective measures.Cultivate disease-resistant variety is the target that various countries silkworm industry scientist lays siege to always.China is since whole nation breeding for disease resistance meeting for the first time in 1981, the relevant scientific research in the whole nation, teaching and production unit are to the main flacherie repellence of silkworm, resistance of environment etc. has been carried out the kind generaI investigation, a large amount of research has been carried out in genetics of resistance rule and resistant variety choice etc. thereof, in disease-resistant mechanism, the genetic analysis of disease-resistant proterties, interracial otherness has obtained certain progress, but the cultivated silkworm breed variety of good disease resistance without comparison still so far, especially anti-NPV kind fails to break through always, main mistaken ideas are the silkworm disease-resistant gene is failed to obtain, for the seed selection of silkworm disease-resistant variety with promote and still be in the research initial period.
Thailand belongs to tropical climate, and high temperature has formed the local cultivated silkworm breed variety that adapts to this area's climatic characteristic for a long time throughout the year, these kinds have high temperature resistance, the characteristics of viral diseases can adapt to tropical and subtropical zone local climate characteristics, and this research department has introduced the high anti-kind NL of Thailand in 2004.Therefore in China's cultivated silkworm breed variety, introduce the disease-resistant gene of safe silkworm kind, seem highly significant.
Summary of the invention:
The purpose of this invention is to provide a kind of method of utilizing Thailand's silkworm disease-resistant gene seed selection domestic silkworm strain resistant to virus disease, utilize the torrid areas cultivated silkworm breed variety to have the distinctive ntiviral characteristic that forms through long-term evolution, introduce disease-resistant gene, transform the good cultivated silkworm breed variety of the existing economic characters of China, improve quality and the proterties of China silkworm.
In order to achieve the above object, the technical solution used in the present invention is as follows:
Utilize the method for Thailand's silkworm disease-resistant gene seed selection domestic silkworm strain resistant to virus disease, it is characterized in that may further comprise the steps:
A) select for use spring and autumn dual-purpose and the existing cultivated silkworm breed variety 871,872 of state do female parent, do male parent with the Thailand torrid zone disease-resistant variety NL that introduces; Hybridization respectively imports disease-resistant gene;
B) the 2nd generation of hybridization and the 3rd generation silkworm adopt to mix respectively and educate raising, hybridize 2 ages of each Dai Congdi, adopt " colony adds food " NPV nuclear polyhedrosis virus, add the method for malicious concentration " by gradient increase in age " and screen; In the 2nd age, add food 5 * 10
4The NPV virus liquid of individual NPV virion/mL screens; The 3rd age colony add the food 5 * 10
5The NPV virus liquid of individual NPV virion/mL screens; The 4th age colony add the food 5 * 10
6The NPV virus liquid of individual NPV virion/mL screens; Add food 5 * 10 the 5th age
7The NPV virus liquid of individual NPV virion/mL screens, and reserves seed for planting in individuality or the moth district of selecting resistance to improve respectively;
C) make recurrent parent with cultivated silkworm breed variety 871 or 872, adopt the antiviral silkworm individuality of having reserved seed for planting to backcross with it respectively, the method that combines with " importing of backcrossing " and " from generation to generation orientation is added poison screening seed selection ", it is F4-F8 generation, with the resistant strain selected and remain as male parent, 871 or 872 parents that backcross respectively, continuous backcross, in simultaneously per generation, adopted " colony adds food " NPV nuclear polyhedrosis virus, adds the method for malicious concentration " by gradient increase in age " and screen to the 9th generation; Promptly backcross 2 ages of each Dai Congdi, in the 2nd age, add food 5 * 10
4The NPV virus liquid of individual NPV virion/mL screens; The 3rd age colony add the food 5 * 10
5The NPV virus liquid of individual NPV virion/mL screens; The 4th age colony add the food 5 * 10
6The NPV virus liquid of individual NPV virion/mL screens; Add food 5 * 10 the 5th age
7The NPV virus liquid of individual NPV virion/mL screens;
D) selecting good, the deflection maternal character of the every achievement of middle selection when reserving seed for planting, screen moth district and the individuality that has the resistance raising again through adding poison simultaneously;
By repeatedly backcrossing and adding the poison screening, to the 9th generation, system has 97.625% maternal inheritance gene, obtains the more stable pure and mild system of NL of genetic character, every economic characters of system and productivity obtain consolidating and improve in the seed selection process, breed purebred 871NL of target and 872NL.
Advantage that the present invention has and good effect:
1. the method that adopts " importing of backcrossing " and " directive breeding from generation to generation " to combine imports existing improved seeds with antiviral gene, and is simple, efficient and convenient.
2. utilize silkworm by the trend of low age to the resistance enhancing at advanced age, gradient is added the poison screening and improved the silkworm resistance, and is workable.
Good, strong good the supporting of resulting antiviral cultivated silkworm breed variety economic characters.
Description of drawings:
Fig. 1 disease-resistant variety imports the block diagram of 871,872 commercial varieties.
Among the figure: represents susceptible proterties, and ■ represents disease-resistant proterties
Embodiment
Utilize the method for Thailand's silkworm disease-resistant gene seed selection domestic silkworm strain resistant to virus disease, it is characterized in that may further comprise the steps:
E) select for use the existing cultivated silkworm breed variety 871 of dual-purpose China in spring and autumn to do female parent, do male parent with the Thailand torrid zone disease-resistant variety NL that introduces; Hybridization imports disease-resistant gene;
F) the 2nd generation of hybridization and the 3rd generation silkworm adopt to mix respectively and educate raising, hybridize 2 ages of each Dai Congdi, adopt " colony adds food " NPV nuclear polyhedrosis virus, add the method for malicious concentration " by gradient increase in age " and screen; In the 2nd age, add food 5 * 10
4The NPV virus liquid of individual NPV virion/mL screens; The 3rd age colony add the food 5 * 10
5The NPV virus liquid of individual NPV virion/mL screens; The 4th age colony add the food 5 * 10
6The NPV virus liquid of individual NPV virion/mL screens; Add food 5 * 10 the 5th age
7The NPV virus liquid of individual NPV virion/mL screens, and reserves seed for planting in individuality or the moth district of selecting resistance to improve respectively;
G) make recurrent parent with cultivated silkworm breed variety 871 or 872, adopt the antiviral silkworm individuality of having reserved seed for planting to backcross with it respectively, the method that combines with " importing of backcrossing " and " from generation to generation orientation is added poison screening seed selection ", it is F4-F8 generation, the resistant strain of selecting and remain is as male parent, 871 or 872 parents that backcross respectively, continuous backcross, in simultaneously per generation, adopted " colony adds food " NPV nuclear polyhedrosis virus, adds the method for malicious concentration " by gradient increase in age " and screen to the 9th generation; Promptly backcross 2 ages of each Dai Congdi, in the 2nd age, add food 5 * 10
4The NPV virus liquid of individual NPV virion/mL screens; The 3rd age colony add the food 5 * 10
5The NPV virus liquid of individual NPV virion/mL screens; The 4th age colony add the food 5 * 10
6The NPV virus liquid of individual NPV virion/mL screens; The 5th age add the food 5 * 10
7The NPV virus liquid of individual NPV virion/mL screens;
H) selecting good, the deflection maternal character of the every achievement of middle selection when reserving seed for planting, screen moth district and the individuality that has the resistance raising again through adding poison simultaneously;
I) by repeatedly backcrossing and adding the poison screening, to the 9th generation, system has 97.625% maternal inheritance gene, obtains the more stable pure and mild system of NL of genetic character, every economic characters of system and productivity obtain consolidating and improve in the seed selection process, breed the purebred 871NL of target.
Select for use spring and autumn dual-purpose and the existing cultivated silkworm breed variety 872 of state do female parent, do male parent with the Thailand torrid zone disease-resistant variety NL that introduces, adopt above-mentionedly to obtain the purebred 872NL of target with optional the educating of quadrat method.
Claims (1)
1, utilize the method for Thailand's silkworm disease-resistant gene seed selection domestic silkworm strain resistant to virus disease, it is characterized in that may further comprise the steps:
A) select for use the existing cultivated silkworm breed variety 871,872 of dual-purpose China in spring and autumn to do female parent, do male parent with the Thailand torrid zone disease-resistant variety NL that introduces; Hybridization respectively imports disease-resistant gene;
B) the 2nd generation of hybridization and the 3rd generation silkworm adopt to mix respectively and educate raising, hybridize 2 ages of each Dai Congdi, adopt " colony adds food " NPV nuclear polyhedrosis virus, add the method for malicious concentration " by gradient increase in age " and screen; In the 2nd age, add food 2～5 * 10
4The NPV virus liquid of individual NPV virion/mL screens; The 3rd age colony add the food 2～5 * 10
5The NPV virus liquid of individual NPV virion/mL screens; The 4th age colony add the food 2～5 * 10
6The NPV virus liquid of individual NPV virion/mL screens; Add food 2～5 * 10 the 5th age
7The NPV virus liquid of individual NPV virion/mL screens;
C) make recurrent parent with cultivated silkworm breed variety 871,872, adopt the antiviral silkworm individuality of having reserved seed for planting to backcross with it respectively, the method that combines with " importing of backcrossing " and " from generation to generation orientation is added poison screening seed selection ", be that F4-F8 is for continuous backcross, adopt " colony adds food " NPV nuclear polyhedrosis virus, add the method for malicious concentration " by gradient increase in age " and screen to the 9th generation; Promptly backcross 2 ages of each Dai Congdi, in the 2nd age, add food 5 * 10
4The NPV virus liquid of individual NPV virion/mL screens; The 3rd age colony add the food 5 * 10
5The NPV virus liquid of individual NPV virion/mL screens; The 4th age colony add the food 5 * 10
6The NPV virus liquid of individual NPV virion/mL screens; Add food 5 * 10 the 5th age
7The NPV virus liquid of individual NPV virion/mL screens;
D) the every achievement of reselection procedure is good, the deflection maternal character selecting when reserving seed for planting, and has moth district and the individuality that resistance improves again through adding the poison screening simultaneously;
E) by repeatedly backcrossing and adding the poison screening, to the 9th generation, system has 97.625% maternal inheritance gene, obtains the more stable pure and mild system of NL of genetic character, every economic characters of system and productivity obtain consolidating and improve in the seed selection process, breed purebred 871NL of target and 872NL.
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Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102392025A (en) * | 2011-11-24 | 2012-03-28 | 西南大学 | Bombyx mori BmSpry gene and application thereof |
| CN102925484A (en) * | 2012-11-21 | 2013-02-13 | 西南大学 | Application and recombinant vector of bombyx mori nuclear polyhedrosis virus polygene inverted repeat sequence and bombyx mori lipase-1 gene |
| CN103070138A (en) * | 2013-02-04 | 2013-05-01 | 江苏科技大学 | Method for breeding hypersilk, bombyx mori densovirus-resistant and fluoride-resistant silkworm variety |
| CN103081872A (en) * | 2013-02-04 | 2013-05-08 | 江苏科技大学 | Method for breeding double-limitation multi-silk-quantity domestic silkworm breed |
| CN104285915A (en) * | 2014-10-31 | 2015-01-21 | 江苏华佳丝绸有限公司 | Cultivation method for subtropic trimolters |
| CN105145500A (en) * | 2015-06-30 | 2015-12-16 | 江苏科技大学 | Bombyxmori Nucleopolyhedrovirus ( BmNPV) full-resistance breeding method |
| CN111004852A (en) * | 2020-01-03 | 2020-04-14 | 江苏科技大学 | Method for screening resistance of bombyx mori nuclear polyhedrosis virus by using BmIML-2 gene |
| CN115053868A (en) * | 2022-07-14 | 2022-09-16 | 云南省农业科学院蚕桑蜜蜂研究所 | Breeding method of practical strain of silkworm with strong nuclear polyhedrosis tolerance |
-
2009
- 2009-05-31 CN CNA2009101169177A patent/CN101558754A/en active Pending
Cited By (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102392025A (en) * | 2011-11-24 | 2012-03-28 | 西南大学 | Bombyx mori BmSpry gene and application thereof |
| CN102925484A (en) * | 2012-11-21 | 2013-02-13 | 西南大学 | Application and recombinant vector of bombyx mori nuclear polyhedrosis virus polygene inverted repeat sequence and bombyx mori lipase-1 gene |
| CN102925484B (en) * | 2012-11-21 | 2014-09-10 | 西南大学 | Application and recombinant vector of bombyx mori nuclear polyhedrosis virus polygene inverted repeat sequence and bombyx mori lipase-1 gene |
| CN103070138A (en) * | 2013-02-04 | 2013-05-01 | 江苏科技大学 | Method for breeding hypersilk, bombyx mori densovirus-resistant and fluoride-resistant silkworm variety |
| CN103081872A (en) * | 2013-02-04 | 2013-05-08 | 江苏科技大学 | Method for breeding double-limitation multi-silk-quantity domestic silkworm breed |
| CN104285915A (en) * | 2014-10-31 | 2015-01-21 | 江苏华佳丝绸有限公司 | Cultivation method for subtropic trimolters |
| CN105145500A (en) * | 2015-06-30 | 2015-12-16 | 江苏科技大学 | Bombyxmori Nucleopolyhedrovirus ( BmNPV) full-resistance breeding method |
| CN105145500B (en) * | 2015-06-30 | 2018-07-27 | 江苏科技大学 | A kind of breeding method of complete anti-silkworm blood type pus illness |
| CN111004852A (en) * | 2020-01-03 | 2020-04-14 | 江苏科技大学 | Method for screening resistance of bombyx mori nuclear polyhedrosis virus by using BmIML-2 gene |
| CN111004852B (en) * | 2020-01-03 | 2023-07-25 | 江苏科技大学 | Method for screening silkworm nuclear polyhedrosis virus disease resistance by using BmIML-2 gene |
| CN115053868A (en) * | 2022-07-14 | 2022-09-16 | 云南省农业科学院蚕桑蜜蜂研究所 | Breeding method of practical strain of silkworm with strong nuclear polyhedrosis tolerance |
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Application publication date: 20091021 |