CN104839099B - A kind of hybridization, the method for the anti-nuclear polyhedrosis of backcross improvement silkworm - Google Patents
A kind of hybridization, the method for the anti-nuclear polyhedrosis of backcross improvement silkworm Download PDFInfo
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- 241000255789 Bombyx mori Species 0.000 title claims abstract description 102
- 238000000034 method Methods 0.000 title claims abstract description 34
- 238000009396 hybridization Methods 0.000 title claims abstract description 14
- 230000003460 anti-nuclear Effects 0.000 title abstract description 13
- 241000409811 Bombyx mori nucleopolyhedrovirus Species 0.000 claims abstract description 28
- 108090000623 proteins and genes Proteins 0.000 claims abstract description 26
- 239000007788 liquid Substances 0.000 claims abstract description 15
- 235000013305 food Nutrition 0.000 claims abstract description 14
- 238000004519 manufacturing process Methods 0.000 claims abstract description 11
- 201000010099 disease Diseases 0.000 claims description 45
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 claims description 45
- 241000257303 Hymenoptera Species 0.000 claims description 12
- 240000000249 Morus alba Species 0.000 claims description 12
- 235000008708 Morus alba Nutrition 0.000 claims description 12
- 241000700605 Viruses Species 0.000 claims description 11
- 238000012216 screening Methods 0.000 claims description 7
- 231100000636 lethal dose Toxicity 0.000 claims description 6
- 238000012545 processing Methods 0.000 claims description 6
- 238000012252 genetic analysis Methods 0.000 claims description 5
- 238000002474 experimental method Methods 0.000 claims description 4
- 230000004083 survival effect Effects 0.000 claims description 4
- 206010011409 Cross infection Diseases 0.000 claims description 3
- 241001671301 Morus nigra Species 0.000 claims description 3
- 206010029803 Nosocomial infection Diseases 0.000 claims description 3
- 230000012447 hatching Effects 0.000 claims description 3
- 238000011835 investigation Methods 0.000 claims description 3
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- 241000196324 Embryophyta Species 0.000 claims 1
- 108091006146 Channels Proteins 0.000 abstract description 3
- 206010020565 Hyperaemia Diseases 0.000 abstract 1
- 208000033809 Suppuration Diseases 0.000 description 7
- 208000035240 Disease Resistance Diseases 0.000 description 6
- 235000013601 eggs Nutrition 0.000 description 5
- 238000005516 engineering process Methods 0.000 description 3
- 238000009366 sericulture Methods 0.000 description 3
- 238000012360 testing method Methods 0.000 description 3
- 230000008901 benefit Effects 0.000 description 2
- 238000009395 breeding Methods 0.000 description 2
- 230000001488 breeding effect Effects 0.000 description 2
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
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Abstract
The invention discloses a kind of with hybridization, the method for the anti-nuclear polyhedrosis of backcross improvement silkworm, circulated using the hyperemia being made up of anti-nuclear polyhedrosis silkworm strain with conventional improved seeds hybridization, backcrossing and three kinds of modes of selfing, progressively the channel genes of Current Good cultivated silkworm breed variety into the silkworm egg matter of anti-nuclear polyhedrosis, add the selection approach of food BmNPV virus liquids using moth area split-half method simultaneously, in the case where keeping anti-BmNPV major gene resistances not lose, its economic characters is improved, to reach the demand of existing production kind.Then anti-nuclear polyhedrosis silkworm strain again with improvement and corresponding Current Good mixing breed, produces first cross kind.The first cross kind of this method production, has the repellence of height to nuclear polyhedrosis, while economic characters are excellent, the rural area popularization and application in national silkworm area are deep to be welcome by silkworm raiser.
Description
Technical field
The present invention relates to a kind of hybridization, the method for the anti-nuclear polyhedrosis of backcross improvement silkworm.
Background technology
Bombyx mori nuclear polyhydrosis virus (Bombyx mori Nuclear Polyhedrosis Virus, BmNPV) is danger
The main virus of evil silkworm, the first place of silkworm virosis is occupied by the nuclear polyhedrosis of its initiation.According to Jiangsu, Sichuan, Zhejiang, wide
The investigation in Xi Dengji grown silkworms area, loss of the pus illness to sericulture accounts for more than the 50% of the sick total losses of silkworm, and some places are up to 80%,
Even there is the severe situation that No kernels or seeds are gathered, as in a year of scarcity, the serious contusion enthusiasm of silkworm raiser hinders the development of silkworm and mulberry cause.For this
Kind of phenomenon, in, day, the sericulture scientific worker of the country such as method study BmNPV, and carried out the anti-BmNPV of silkworm from different perspectives
Breeding research, has made some progress, but the practical level of these disease-resistant varieties is not enough, it is impossible to which play has aborning
The effect of effect." state of Chinese Academy of Agricultural Sciences eggs of silkworm matter resource conservation " center " carries out silkworm germ plasm resource and resisted for Jiangsu University of Science and Technology
During virus screening, it is found that eggs of silkworm confrontation BmNPV premunition is very strong, genetic analysis shows that its disease resistance is by dominant master
Gene control is imitated, but economic phenomena is very poor, the existing Silkworm varieties of China is can not show a candle to, aborning without direct value, it is necessary to
Breeding improvement is carried out to the germplasm.
The content of the invention
Goal of the invention：In order to overcome the shortcomings of the anti-pus illness ability of existing silkworm commercial variety, the present invention provide a kind of hybridization,
The method of the anti-nuclear polyhedrosis of backcross improvement silkworm, the Silkworm varieties of incubation have to BmNPV height repellences.
Technical scheme：In order to solve the above technical problems, a kind of hybridization of the present invention, the anti-nuclear polyhedrosis of backcross improvement silkworm
Method,
1) the anti-BmNPV investigation of silkworm germ plasm resource is carried out, specific method is：
A) the different cultivars silkworm seed of preservation is normally hastened the hatching of silkworms, mixing ant amount is collected ants, and 0.4g ant amounts, new fresh mulberry leaf are received per kind
Raise to 1 age and sleep；When silkworm from 2 ages, per the area of kind number 50 × 9, it is placed in culture dish, it is stand-by.
B) 5 grades of virus concentration gradients and a blank control are set, the multiple of graded concentrations gradient is generally 10 times, successively
Dilute step by step downwards；The maximum concentration for adding food NPV viruses is 1.85 × 109PIB/ml, is diluted downwards step by step with 10 times, minimum dense
Spend for 1.85 × 105PIB/ml, totally 5 grades of concentration；Each concentration sets 3 repetitions, each 50 silkworms in duplicate block.
C) same to kind, the mulberry leaf with the food value of leaf are chosen, break into a diameter of 3 centimetres sequin mulberry leaf (avoid getting to big vein,
Small holes caused by worms and the mulberry leaf folded, it is ensured that every leaf area equation).
D) virus liquid for drawing 0.1ml with liquid-transfering gun is equably applied on circular blade, and feeding 5 per area silkworm adds malicious leaf；
E) use common mulberry leaf instead again after silkworm eats up add malicious leaf completely to raise, normal molting processing.
F) 8 points of every morning, the situation of growing of 2 pm observed and recorded silkworm, once sick silkworm occur will choose in time,
It to avoid cross-infection.
G) with spss v16.0 processing statistics, the LC50 of each experiment kind is obtained.Obtain (the name of 1 silkworm resource
For R) anti-BmNPV abilities it is very strong, from its 2 age silkworm to BmNPV half lethal concentration (LC50) up to 109PIB/ml, is common
More than 1000 times of silkworm；
2) the anti-BmNPV performances to germplasm R carry out genetic analysis, as a result show its anti-BmNPV performance by 1 dominant base
Because of control, but other economic characters of the germplasm are very poor；
3) the female silkworms of R containing anti-nuclear polyhedrosis gene and the male silkworm of conventional improved seeds are hybridized, produces F1 generation；
4) F1 generation is raised in mixing, and the male silkworm by the female silkworm of F1 generation again with conventional improved seeds is returned, and produces BC1 generations；
5) in BC1 generations, are raised in mixing, when collecting ants just with 1 × 107PIB/ml BmNPV virus liquids screening, non-anti- individual is dead
Die, disease-resistant individual survival；Select the male moth of the excellent disease-resistant female conventional improved seeds of moth individual backcrossing of economic characters in BC1, production
Raw BC2 generations；
6 with starting points of the BC2 on behalf of new anti-pus illness kind, repeats the above steps 5), carries out continuous backcross and disease resistance choosing
Select, to ensure while pus illness kind that the excellent economic characters channel genes of current race are anti-during subculture of reserving seed for planting again not
Lose disease-resistant gene；
7) after 4 times are returned, the economic characters of disease-resistant variety are greatly improved, and now disease-resistant gene is heterozygosis
State, the homozygosis of disease-resistant major gene resistance is then carried out according to dominant single-gene homozygosis fixing means, disease-resistant gene homozygosis is selected small
It is (moth area)；Specific method is：To backcrossing offspring's (being set to B1) of 4 times, using selfing more than 20 single moths of method production after
Generation, as B2 generations, then all 20 Ge E areas collected ants using moth area split-half method list moth, newly-hatched silkworm just freshens food 1 × 107PIB/ml's
BmNPV virus liquids, specifically add malicious method for example c), d), e), the silkworm body developmental state in observation Ge E areas；If which moth area has extremely
Individual is died, then eliminates the moth area, if the moth area does not have dead individuals, retains male and female selfing in the moth area, Qie Tonge areas and stay
Kind, at least produce 10 single moth subcultures, as B3 generations；B3 is collected ants using moth area split-half method list moth again for each moth area, and newly-hatched silkworm adds
Poison, specifically adds malicious method for example c), d), e), the silkworm body developmental state in observation Ge E areas；If having dead individuals in some moth area
The moth area is eliminated, if all individuals can survive in some moth area, the disease-resistant gene for showing the moth area is in homozygosis, moth area
All newly-hatched silkworms can resist BmNPV and grow up healthy and sound, and this moth area is exactly the small system of disease-resistant homozygosis；It is general to result in homozygosis in B4 generations
Small system.
8) economic characters are further improved, the improvement of silkworm disease-resistant variety is completed for starting point with the small system of disease-resistant gene homozygosis.
Beneficial effect：The present invention has advantages below in terms of existing technologies：
Because the economic benefit of disease-resistant variety has reached the level of existing commercial variety, and disease-resistant gene is dominant pure
Close, the height repellence to nuclear polyhedrosis can be kept with the first-filial generation after other mixing breeds, the present invention passes through spy
Unstable anti-BmNPV performances germplasm R is screened and is prepared last property by distinctive hybridizing method by some methods
The stable anti-nuclear polyhedrosis silkworm of energy, simple to operate, the scale of selection and improvement is smaller, and economic characters are improved quickly.By the party
The first cross kind that the anti-nuclear polyhedrosis kind that method is bred as is made is deep to be welcome by silkworm raiser in rural area popularization and application.
Brief description of the drawings
Fig. 1 is hybridization, the anti-BmNPV silkworms germplasm schematic diagram of backcross improvement.
Embodiment
The present invention is further described below in conjunction with the accompanying drawings.
1st, the screening of disease-resistant pus illness silkworm germplasm
A) silkworm seed of normal different cultivars of hastening the hatching of silkworms, turns mixing ant amount after green grass or young crops and collects ants, 0.3~0.4g, fresh mulberry are received per kind
Leaf, which was raised to 1 age, sleeps；When silkworm from 2 ages, it is placed in by 50 for a test block in culture dish, is arranged as required to certain area
Number, it is stand-by.
B) maximum concentration that general disease-resistant variety adds food BmNPV is 109PIB/ml (rather than disease-resistant variety adds food BmNPV's
Maximum concentration is 108PIB/ml), diluted step by step downwards with 6 times, least concentration is 106PIB/ml (non-disease-resistant variety least concentrations
For 105PIB/ml), totally 5 grades of concentration, each concentration sets 3 repetitions.
C) same to kind, the mulberry leaf with the food value of leaf are chosen, break into a diameter of 3 centimetres sequin mulberry leaf (avoid getting to big vein,
Small holes caused by worms and the mulberry leaf folded, it is ensured that every leaf area equation).
D) virus liquid for drawing 0.1ml with liquid-transfering gun is equably applied on circular blade, and feeding 5 per area silkworm adds malicious leaf；
E) use common mulberry leaf instead again after silkworm eats up add malicious leaf completely to raise, normal molting processing.
F) 8 points of every morning, the situation of growing of 2 pm observed and recorded silkworm, once sick silkworm occur will choose in time,
It to avoid cross-infection.
G) with spss v16.0 processing statistics, the LC50 of each experiment kind is obtained, (the name of 1 silkworm resource is obtained
For R), from its 2 age silkworm to BmNPV LC50 up to 109PIB/ml。
Hybridization is realized by the following method to it after screening：
1) the anti-BmNPV performances to germplasm R carry out genetic analysis, as a result show its anti-BmNPV performance by 1 dominant base
Because of control, but other economic characters of the germplasm are very poor；
2) the female silkworms of R containing anti-nuclear polyhedrosis gene and the male silkworm of conventional improved seeds are hybridized, produces F1 generation；
3) F1 generation is raised in mixing, and the male silkworm by the female silkworm of F1 generation again with conventional improved seeds is returned, and produces BC1 generations；
4) in BC1 generations, are raised in mixing, when collecting ants just with 1 × 107PIB/ml BmNPV virus liquids screening, non-anti- individual is dead
Die, disease-resistant individual survival；Select the male moth of the excellent disease-resistant female conventional improved seeds of moth individual backcrossing of economic characters in BC1, production
Raw BC2 generations；
5) with starting points of the BC2 on behalf of new anti-pus illness kind, repeat the above steps 4), carry out continuous backcross and disease resistance choosing
Select, to ensure while pus illness kind that the excellent economic characters channel genes of current race are anti-during subculture of reserving seed for planting again not
Lose disease-resistant gene；
6) after 4 times are returned, the economic characters of disease-resistant variety are greatly improved, and now disease-resistant gene is heterozygosis
State, the homozygosis of disease-resistant major gene resistance is then carried out according to dominant single-gene homozygosis fixing means, disease-resistant gene homozygosis is selected small
It is (moth area)；Specific method is：To backcrossing offspring's (being set to B1) of 4 times, using selfing more than 20 single moths of method production after
Generation, as B2 generations, then all 20 Ge E areas collected ants using moth area split-half method list moth, newly-hatched silkworm just freshens food 1 × 107PIB/ml's
BmNPV virus liquids, specifically add malicious method for example c), d), e), the silkworm body developmental state in observation Ge E areas；If which moth area has extremely
Individual is died, then eliminates the moth area, if the moth area does not have dead individuals, retains male and female selfing in the moth area, Qie Tonge areas and stay
Kind, at least produce 10 single moth subcultures, as B3 generations；B3 is collected ants using moth area split-half method list moth again for each moth area, and newly-hatched silkworm adds
Poison, specifically adds malicious method for example c), d), e), the silkworm body developmental state in observation Ge E areas；If having dead individuals in some moth area
The moth area is eliminated, if all individuals can survive in some moth area, the disease-resistant gene for showing the moth area is in homozygosis, moth area
All newly-hatched silkworms can resist BmNPV and grow up healthy and sound, and this moth area is exactly the small system of disease-resistant homozygosis；It is general to result in homozygosis in B4 generations
Small system.
7) economic characters are further improved, the improvement of silkworm disease-resistant variety is completed for starting point with the small system of disease-resistant gene homozygosis.
2nd, the genetic analysis model of disease-resistant system's silkworm egg matter
It is silkworm egg matter (being set to S) by disease-resistant system's silkworm egg matter (being set to R) and sensitivity, is configured to following F1, F2, BC1 etc., then
Using quantitatively food method is freshened, all colonies are screened with BmNPV virus liquids, each colony are obtained to BmNPV repellence (with half
Lethasl concentration take the logarithm value expression), such as table 1：
The Resistant gerplasm of table 1 and its hybridization, backcross progeny are analyzed BmNPV repellence
The result studied according to above experiment value and its forefathers, may infer that the disease-resistant silkworm germplasm R to the disease-resistant of BmNPV
Property controlled by pair of dominant genes on autosome, it is assumed that the gene be A, then Resistant gerplasm R genotype be AA；Sensitivity system
Germplasm S genotype is then aa, and the genotype of first-filial generation is Aa.
3rd, Backcrossing methods improve the economic characters of anti-BmNPV silkworms
3.1 modification methods
With the male hybridization of disease-resistant system's germplasm R female and current race, the generation of male continuous backcross 3 of current race is then used again,
So the genetic constitution of current race accounts for more than 90% in backcross progeny colony.Assuming that current race is M, it hybridizes, backcrossing is shown
It is intended to as shown in Figure 1.
Each backcross generations (genotype is Aa, aa) are with 1 × 107PIB/ml BmNPV virus liquids are screened, and are owned
Genotype is dead for aa individual, and genotype is then survived for Aa individual, and the good individual of selection economic characters is reserved seed for planting subculture；
Since B1 (genotype is Aa) from generation to generation, carry out resistance to ospc gene homozygosis and fix, by initial survey, reinspections, the resistance to disease of identification homozygosis fixation
Gene.To B4 from generation to generation, after testing and verify determination, detect the resistance to small system of ospc gene homozygosis, now the genotype of the small system of homozygosis is AA.
Economic characters raising is carried out afterwards, and reinforcing cocoon quality, silk quality character, increase raise moth area and moth area of reserving seed for planting, expands range of choice,
Individual choice is carried out on the basis of the selection of moth area.And it is stable to target economic characters, the resistance to sick strain of incubation is shown to BmNPV
Height repellence, and cocoon, silk quality reach current race level.
3.2 biological examples
With Resistant gerplasm R, to existing production cultivated silkworm breed variety " 871 ", (871,872 be the Chinese Academy of Agricultural Sciences as previously described
The spring and autumn dual-purpose Silkworm varieties that sericulture research institute is bred as in the enforcement period of the ninth five-year plan, the large area such as present Sichuan, Yunnan, Shandong, Shanxi is pushed away
Extensively, it is the most spring and autumn dual-purpose kind of southwest popularization amount.) made after 4 congested improvement, self cross and selection is then carried out,
Point-score Dan E areas are done using moth area when self cross and selection to collect ants, and newly-hatched silkworm just add food virus liquid method, selection resistance regardless of
From moth area reserve seed for planting, then carry out in moth area of reserving seed for planting individual choice, improve the Cocoon filament characters of original seed.After 4 generation self cross and selections, shape
Stablize and reach the disease-resistant varieties of practical level into economic characters, be named 871R.From spring in 2009, with 871R and existing life
Production is configured to cross combination with cultivated silkworm breed variety " 872 ", and it is bred silkworms, Silk Reeling is shown in Table 2, as seen from Table 2 new cross combination " 871R
" 871 × 872 " result is suitable, and indices mutual just, has reached practical with former cross combination for × 872 " result
Level.
The new germ plasm 871R of table 2 raising result (laboratory)
Autumn in 2009, disease resistance detection is carried out to new germ plasm 871R and its cross combination, testing result is shown in Table 3, can by table 3
See, the disease resistance of either original seed 871R or first cross kind 871R × 872 has met or exceeded the anti-of former Resistant gerplasm R
Characteristic of disease, has height repellence to BmNPV, meets Production requirement.
The new germ plasm 871R of table 3 disease resistance detection (laboratory)
The above is only the preferred embodiment of the present invention, it should be pointed out that：For the ordinary skill people of the art
For member, under the premise without departing from the principles of the invention, some improvements and modifications can also be made, these improvements and modifications also should
It is considered as protection scope of the present invention.
Claims (3)
1. it is a kind of with hybridization, the method for the anti-BmNPV silkworms germplasm of backcross improvement, it is characterised in that：
1) carry out the anti-BmNPV investigation of silkworm germ plasm resource, obtain the germplasm R of anti-BmNPV abilities very strong silkworm resource；
2) hybridized with anti-BmNPV silkworm germplasm R female silkworm and the male silkworm of conventional improved seeds, produce F1 generation；
3) F1 generation is raised in mixing, and the female silkworm of the excellent F1 generation individual of selection economic characters hybridizes with conventional variety again, produces BC1
In generation, complete the congested circulation of a backcrossing；
4) BC1 generation mixing is collected ants, and newly-hatched silkworm orally adds food BmNPV virus liquids, and susceptible individual is dead, disease-resistant individual survival；Selection warp
The excellent disease-resistant individual female silkworms of BC1 of character of helping hybridize with conventional variety again, produce BC2；
5) using BC2 as starting point, repeat the above steps 4), continuously improved, led with the excellent economic characters for ensureing current race
Disease-resistant gene is not lost while entering Resistant gerplasm；
6) individual left after continuous backcross 4 times through adding food BmNPV virus liquids is set to B1 generations, and B1 selfings are produced into B2 generations, B2 generations
Produce at least 20 single moths；
7) B2 is collected ants for all moth areas using moth area split-half method, i.e., all moth areas, which are divided into two, takes half moth individually to raise, and newly-hatched silkworm is used
BmNPV virus liquids are screened, and are occurred dead moth area and are eliminated without exception, the male and female selfing in the Qu Tonge areas of moth area of the whole survivals of individual
The production of hybrid seeds, produces B3 generations, and 10 single moths are at least stayed per moth area；
8) in B3 generations, continue to use step 7) shown in method, anti-BmNPV screenings are carried out to every 1 Ge E areas, each individual are left anti-
Bing E areas, now all individual disease-resistant gene homozygosis in moth area, set up the disease-resistant small system of disease-resistant gene homozygosis, other to have death
The moth area of individual is then all eliminated；
9) selection of the disease-resistant small system of gene pure again Jing Guo economic characters, preferentially reserves seed for planting；With corresponding conventional improved seeds pair
Hand over to plant and hybridized, the first cross kind of production carries heterozygosis disease-resistant gene, has height repellence to nuclear polyhedrosis；
The screening step of the germplasm R is：
A) the different cultivars silkworm seed of preservation is normally hastened the hatching of silkworms, mixing ant amount is collected ants, 0.4g ant amounts is received per kind, new fresh mulberry leaf is raised
Slept to 1 age；When silkworm from 2 ages, per the area of kind number 50 × 9, it is placed in culture dish, it is stand-by；
B) 5 grades of virus concentration gradients and a blank control are set, the multiple of graded concentrations gradient is generally 10 times, successively downwards
Dilute step by step；The maximum concentration for adding food NPV viruses is 1.85 × 109PIB/ml, is diluted downwards, least concentration is step by step with 10 times
1.85×105PIB/ml, totally 5 grades of concentration；Each concentration sets 3 repetitions, each 50 silkworms in duplicate block；
C) same to kind, the mulberry leaf with the food value of leaf are chosen, a diameter of 3 centimetres of sequin mulberry leaf are broken into；
D) virus liquid for drawing 0.1ml with liquid-transfering gun is equably applied on circular blade, and feeding 5 per area silkworm adds malicious leaf；
E) use common mulberry leaf instead again after silkworm eats up add malicious leaf completely to raise, normal molting processing；
F) 8 points of every morning, the situation of growing of 2 pm observed and recorded silkworm, once sick silkworm occur will choose in time, in order to avoid
Generation cross-infection；
G) with spss v16.0 processing statistics, the LC50 of each experiment kind is obtained, BmNPV half lethal concentration is obtained
(LC50) up to 109PIB/ml, be common silkworm more than 1000 times are germplasm R.
2. hybridization according to claim 1, the method for the anti-BmNPV silkworms germplasm of backcross improvement, it is characterised in that：To germplasm
R anti-BmNPV performances carry out genetic analysis, and anti-BmNPV performances are controlled by 1 dominant gene.
3. hybridization according to claim 1, the method for the anti-BmNPV silkworms germplasm of backcross improvement, it is characterised in that：It is described
BmNPV virus liquids concentration is 1 × 107PIB/ml。
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| CN107821342B (en) * | 2017-11-30 | 2020-04-14 | 贵州省蚕业研究所 | Breeding method of silkworm with multiple silks and capable of resisting blood type pyosis |
| CN109364053B (en) * | 2018-11-20 | 2020-08-25 | 江苏科技大学 | Application of small molecular compound in preparation of silkworm baculovirus infection resisting medicine |
| CN111004852B (en) * | 2020-01-03 | 2023-07-25 | 江苏科技大学 | Method for screening silkworm nuclear polyhedrosis virus disease resistance by using BmIML-2 gene |
| CN115088680B (en) * | 2022-07-04 | 2023-06-06 | 浙江省农业科学院 | Breeding method of silkworm blood-type sepsis-resistant variety |
| CN115053868A (en) * | 2022-07-14 | 2022-09-16 | 云南省农业科学院蚕桑蜜蜂研究所 | Breeding method of practical strain of silkworm with strong nuclear polyhedrosis tolerance |
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| SU644430A2 (en) * | 1977-08-23 | 1979-01-30 | Главное Управления Шелководства Министерства Сельского Хозяйства Узбекской Сср | Method of obtaining silkworm eggs |
| BG30945A1 (en) * | 1980-08-06 | 1981-10-15 | Petkov | Silkworm hybrid <vratza 2l-121> and back crossbreeding |
| CN101427668B (en) * | 2008-10-31 | 2011-06-01 | 江苏科技大学 | Breeding method for densovirus disease resistant and fluorine resistant cultivated silkworm breed variety |
| CN101427667B (en) * | 2008-10-31 | 2011-12-07 | 江苏科技大学 | Breeding method for cultivated silkworm breed variety of fluorine resistant larva and non-palea perfect insect wing |
| JP5839810B2 (en) * | 2011-03-03 | 2016-01-06 | 日本製粉株式会社 | Transgenic silkworm producing fibrinogen |
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