CN103110632B - Composition for treating nosemosis of bees and preparation method thereof - Google Patents

Abstract

The invention relates to a composition for treating nosemosis of bees, which is formed by uniformly mixing the following components in parts by weight: 10-20 parts of citric acid, 2-8 parts of ciprofloxacin hydrochloride, 2-5 parts of quinuronium metilsulfate and 67-86 parts of glucose. The composition disclosed by the invention has the beneficial effects of reasonable components, simple preparation and obvious curative effect. Clinical application proves that the composition has special effect on treating the nosemosis of the bees.

Description

A kind of composition and method of making the same for the treatment of Apis sporidiosis

Technical field

The present invention relates to treat the composition and method of making the same of Apis sporidiosis, belong to animal field of medicaments, particularly a kind of composition and method of making the same that is specifically designed to treatment Apis sporidiosis.

Background technology

Apis sporidiosis has another name called pebrine, is a kind of adult honeybee infectious disease of the digestive tract being caused by Nosema apis worm, take dysentery, middle intestinal edema, nonelastic, be canescence as feature, OIE is classified as category-B epidemic disease.Ill Apis initial stage non-evident sympton, later stage is because sporozoon has been destroyed Apis intestinal mucosa, there is digestive function obstacle, malnutrition in sick honeybee, dispirited, blackout, wing are shaken end to end, can not circle in the air, the symptom such as creep everywhere, middle intestinal expands, and is canescence, arrange buff and brown with the feces of stench taste, and with dysentery.

This disease spreads all over more than 100 countries of five continents, is widely distributed.At present, treat this disease the most frequently used be various anti-parasite medicines, owing to being mostly that folk prescription or prescription are unreasonable, clinical application effect is not fine.

Therefore researching and developing a kind of specific medicament for the treatment of Apis sporidiosis is one of this field scientific research technical staff new problem anxious to be resolved.

Summary of the invention

The object of the invention is to overcome above-mentioned weak point, provide that a kind of reasonable recipe, preparation are simple, successful, effectively treat the composition and method of making the same of Apis sporidiosis.

Implementation of the present invention is as follows for achieving the above object: a kind of compositions for the treatment of Apis sporidiosis, by following component by ratio of weight and the number of copies evenly mixed preparing form: citric acid 10-20 part, ciprofloxacin 2-8 part, quinoline sulfate urea 2-5 part, glucose 67-86 part.

Described various component optimum ratio amount is: 15 parts of citric acids, 5 parts of ciprofloxacins, 3.5 parts of quinoline sulfate ureas, 76.5 parts of glucoses.

Treat a preparation method for the compositions of Apis sporidiosis, carry out as follows:

(1) take by weight citric acid 10-20 part, glucose 10-20 part, mix 5-10 minute to even;

(2) take by weight ciprofloxacin 2-8 part, quinoline sulfate urea 2-5 part, glucose 4-13 part, mix 5-10 minute to even;

(3) mixture that combining step (1) and (2) make, adds glucose surplus part, mixes 10～15 minutes extremely evenly and get final product.

The medicinal property of each component is as follows:

Citric acid: sweet, sour, bitter, return lung, spleen, the heart, Liver Channel, there is the function of nourishing the liver hepatoprotective, hemostasis antidiarrheal, dampness removing to stop itchin, removing toxic substances parasite killing, dispersing swelling and dissipating binds; Cure mainly abdominal pain due to worm stagnation, incessant chronic diarrhea, have blood in stool, let out after dysentery heavily etc.

Ciprofloxacin: have broad-spectrum antibacterial action, escherichia coli, bacillus pyocyaneus, Streptococcus hemolyticus etc. are had to stronger antibacterial activity, antibacterial action mechanism is anti-bacteria DNA helicase, and antibacterial action is antibacterial type.

Quinoline sulfate urea: the anti-sporozoon medicine for good, has preventive and therapeutic action concurrently.

All kinds of medicine, for the pathogenic microorganism of Apis sporidiosis and the secondary infection causing thus, is brought into play the effect for the treatment of both the principal and secondary aspects of a disease above.

The invention has the beneficial effects as follows: reasonable recipe, preparation is simple, treating both the principal and secondary aspects of a disease, curative effect highly significant.Clinical practice proves that treatment Apis sporidiosis is had to specially good effect.

The specific embodiment

Below in conjunction with preferred embodiment, to according to the specific embodiment provided by the invention, details are as follows:

Embodiment 1

A kind of composition and method of making the same for the treatment of Apis sporidiosis: carry out as follows: (every part by 10g)

(1) take by weight 100 parts of citric acids, 100 parts of glucoses, mix 5-10 minute to even;

(2) take by weight 20 parts of ciprofloxacins, 20 parts of quinoline sulfate ureas, 40 parts of glucoses, mix 5-10 minute to even;

(3) mixture that combining step (1) and (2) make, adds glucose surplus part, mixes 10～15 minutes extremely evenly and get final product.

Embodiment 2

A kind of composition and method of making the same for the treatment of Apis sporidiosis: carry out as follows: (every part by 10g)

(1) take by weight 150 parts of citric acids, 150 parts of glucoses, mix 5-10 minute to even;

(2) take by weight 50 parts of ciprofloxacins, 35 parts of quinoline sulfate ureas, 85 parts of glucoses, mix 5-10 minute to even;

(3) mixture that combining step (1) and (2) make, adds glucose surplus part, mixes 10～15 minutes extremely evenly and get final product.

Embodiment 3

A kind of composition and method of making the same for the treatment of Apis sporidiosis: carry out as follows: (every part by 10g)

(1) take by weight 20 parts of citric acids, 20 parts of glucoses, mix 5-10 minute to even;

(2) take by weight 80 parts of ciprofloxacins, 50 parts of quinoline sulfate ureas, 130 parts of glucoses, mix 5-10 minute to even;

(3) mixture that combining step (1) and (2) make, adds glucose surplus part, mixes 10～15 minutes extremely evenly and get final product.

Clinical trial:

It is the ill Apis of Apis sporidiosis that clinical experiment animal is selected Laboratory Diagnosed.The isolation of ill honeybee is raised separately, be divided at random 3 groups, every group of 3 casees totally 15 spleen honeybees, 3 groups of feeding environments are identical, and experimental period is carried out 5 days altogether; Blank group is left intact, and medicine matched group is used treatment by institute's control drug by its description; Present composition group is mixed homogeneously with Mel product of the present invention (the prepared compositions of the embodiment of the present invention 1) in 1: 1000 ratio, feed every night 1 time, for three days on end.Computational methods: mortality rate=dead animal number/experimental animal number * 100%, cure rate=healing number of animals/experimental animal number * 100%.Result (testing the 3rd day) when following table is experiment end.

Table is the applicable cases contrast of the present invention and other medicines treatment Apis sporidiosis:

Numbering	Group	Mortality rate %	Cure rate %	During end, there is symptom rate %
					I	Blank group	20	50	30
II	Apis sporidiosis syrup group	9	80	11
					III	Present composition group	4	91	5

This clinical effectiveness statistical analysis, present composition treatment group is being compared difference extremely significantly (P < 0.01) with Apis sporidiosis syrup group aspect cure rate.

Safety testing:

Choose 1 case 5 spleen of healthy Apis, according to 5 times of the made compositions recommended dose of the embodiment of the present invention 1, feed 3 times, during medication, the obviously not bad or Deviant Behavior performance of healthy Apis, to compare difference not remarkable with blank group (being not administration of health group).

Above-mentioned detailed description of the composition and method of making the same of this treatment Apis sporidiosis being carried out with reference to embodiment; illustrative rather than determinate; can list several embodiment according to institute's limited range; therefore in the variation and the modification that do not depart under general plotting of the present invention, within should belonging to protection scope of the present invention.

Claims (3)

1. treat a compositions for Apis sporidiosis, it is characterized in that evenly being mixed by ratio of weight and the number of copies by following component: citric acid 10-20 part, ciprofloxacin 2-8 part, quinoline sulfate urea 2-5 part, glucose 67-86 part.

2. the compositions for the treatment of Apis sporidiosis according to claim 1, is characterized in that evenly being mixed by ratio of weight and the number of copies by following component: 15 parts of citric acids, 5 parts of ciprofloxacins, 3.5 parts of quinoline sulfate ureas, 76.5 parts of glucoses.

3. a preparation method for the compositions for the treatment of according to claim 1 Apis sporidiosis, is characterized in that carrying out as follows:

(1) take by weight citric acid 10-20 part, glucose 10-20 part, mix 5-10 minute to even;

(2) take by weight ciprofloxacin 2-8 part, quinoline sulfate urea 2-5 part, glucose 4-13 part, mix 5-10 minute to even;

(3) mixture that combining step (1) and (2) make, adds glucose surplus part, mixes 10～15 minutes extremely evenly and get final product.