CN103087904A - 用于研究分子的设备和方法 - Google Patents
用于研究分子的设备和方法 Download PDFInfo
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- CN103087904A CN103087904A CN2012102917016A CN201210291701A CN103087904A CN 103087904 A CN103087904 A CN 103087904A CN 2012102917016 A CN2012102917016 A CN 2012102917016A CN 201210291701 A CN201210291701 A CN 201210291701A CN 103087904 A CN103087904 A CN 103087904A
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- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
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- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
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- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Automatic Analysis And Handling Materials Therefor (AREA)
Abstract
Description
Claims (10)
Applications Claiming Priority (2)
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GBGB0717150.7A GB0717150D0 (en) | 2007-09-04 | 2007-09-04 | Apparatus and method |
GB0717150.7 | 2007-09-04 |
Related Parent Applications (1)
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CN2008801144811A Division CN101842692B (zh) | 2007-09-04 | 2008-09-04 | 设备和方法 |
Publications (1)
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CN103087904A true CN103087904A (zh) | 2013-05-08 |
Family
ID=38640188
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CN2008801144811A Expired - Fee Related CN101842692B (zh) | 2007-09-04 | 2008-09-04 | 设备和方法 |
CN2012102917016A Pending CN103087904A (zh) | 2007-09-04 | 2008-09-04 | 用于研究分子的设备和方法 |
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CN2008801144811A Expired - Fee Related CN101842692B (zh) | 2007-09-04 | 2008-09-04 | 设备和方法 |
Country Status (9)
Country | Link |
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US (3) | US8865455B2 (zh) |
EP (1) | EP2188618B1 (zh) |
JP (2) | JP2010538290A (zh) |
CN (2) | CN101842692B (zh) |
BR (1) | BRPI0816426A2 (zh) |
CA (1) | CA2698876A1 (zh) |
ES (1) | ES2617089T3 (zh) |
GB (1) | GB0717150D0 (zh) |
WO (1) | WO2009030953A1 (zh) |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN109387651A (zh) * | 2017-08-08 | 2019-02-26 | 豪夫迈·罗氏有限公司 | 实验室仪器基板 |
Families Citing this family (47)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP3543357A1 (en) | 2007-05-08 | 2019-09-25 | Trustees of Boston University | Chemical functionalization of solid-state nanopores and nanopore arrays and applications thereof |
GB0717150D0 (en) * | 2007-09-04 | 2007-10-17 | Univ Warwick | Apparatus and method |
EP2196796A1 (en) * | 2008-12-09 | 2010-06-16 | Imec | Single molecule optical spectroscopy in solid-state nanopores in a transmission-based approach |
CA2808576A1 (en) | 2009-09-30 | 2011-04-07 | Quantapore, Inc. | Ultrafast sequencing of biological polymers using a labeled nanopore |
US8748091B2 (en) * | 2009-12-18 | 2014-06-10 | The Board Of Trustees Of The University Of Illinois | Characterizing stretched polynucleotides in a synthetic nanopassage |
AU2011218847B2 (en) * | 2010-02-23 | 2015-03-12 | Christopher Gordon Atwood | Methods for the detection of biologically relevant molecules and their interaction characteristics |
US10010272B2 (en) | 2010-05-27 | 2018-07-03 | Profusa, Inc. | Tissue-integrating electronic apparatus |
EP2402738A3 (en) * | 2010-07-02 | 2012-01-11 | Imec | Spectroscopy using nanopore cavities |
CN103069267B (zh) * | 2010-09-29 | 2016-05-11 | 株式会社日立高新技术 | 生物聚合物的光学的解析装置以及方法 |
JP5827999B2 (ja) | 2010-10-06 | 2015-12-02 | プロフューザ,インコーポレイティド | 組織集積センサー |
US9274053B2 (en) * | 2011-05-18 | 2016-03-01 | Uvic Industry Partnerships Inc. | Flow through metallic nanohole arrays |
JPWO2012165400A1 (ja) * | 2011-06-03 | 2015-02-23 | 株式会社日立ハイテクノロジーズ | 生体ポリマーの光学的解析装置及び方法 |
GB2493397A (en) | 2011-08-05 | 2013-02-06 | Base4 Innovation Ltd | Pulse counter for single photon events |
US9007576B2 (en) | 2011-08-12 | 2015-04-14 | Savannah River Nuclear Solutions, Llc | Surface enhanced Raman scattering spectroscopic waveguide |
US11053535B2 (en) | 2011-09-12 | 2021-07-06 | The University Of North Carolina At Chapel Hill | Devices with a fluid transport nanochannel intersected by a fluid sensing nanochannel and related methods |
US9696277B2 (en) * | 2011-11-14 | 2017-07-04 | The Regents Of The University Of California | Two-chamber dual-pore device |
GB2497784A (en) | 2011-12-20 | 2013-06-26 | Base4 Innovation Ltd | Identification of polymers using nanopore devices |
JP6339024B2 (ja) * | 2012-02-10 | 2018-06-06 | ザ ユニバーシティ オブ ノース カロライナ アット チャペル ヒルThe University Of North Carolina At Chapel Hill | 流体ナノファンネルを有する装置、関連する方法、製造及び解析システム |
GB201212135D0 (en) * | 2012-07-09 | 2012-08-22 | Base4 Innovation Ltd | Improved sequencing apparatus |
WO2014062719A2 (en) | 2012-10-15 | 2014-04-24 | Nanocellect Biomedical, Inc. | Systems, apparatus, and methods for sorting particles |
US9651539B2 (en) | 2012-10-28 | 2017-05-16 | Quantapore, Inc. | Reducing background fluorescence in MEMS materials by low energy ion beam treatment |
GB201301178D0 (en) * | 2013-01-23 | 2013-03-06 | Dynamic Biosensors Gmbh | Method for sequencing a template nucleic acid immobilized on a substrate |
WO2014134095A1 (en) | 2013-02-28 | 2014-09-04 | The University Of North Carolina At Chapel Hill | Nanofluidic devices with integrated components for the controlled capture, trapping, and transport of macromolecules and related methods of analysis |
NZ711033A (en) | 2013-03-13 | 2020-03-27 | Univ North Carolina Chapel Hill | Nanofluidic devices for the rapid mapping of whole genomes and related systems and methods of analysis |
WO2014158988A1 (en) | 2013-03-14 | 2014-10-02 | Profusa, Inc. | Method and device for correcting optical signals |
JP6063603B2 (ja) * | 2013-03-14 | 2017-01-18 | ヒューレット−パッカード デベロップメント カンパニー エル.ピー.Hewlett‐Packard Development Company, L.P. | 物質を検出するための装置及び該装置を製造する方法 |
GB201306447D0 (en) * | 2013-04-09 | 2013-05-22 | Base4 Innovation Ltd | Plasmonic detector |
GB201306446D0 (en) * | 2013-04-09 | 2013-05-22 | Base4 Innovation Ltd | Nanopore plasmonic detector |
DK3004378T3 (en) | 2013-05-24 | 2018-03-26 | Illumina Cambridge Ltd | Pyrophosphorolytic sequencing using nanopores |
US9862997B2 (en) | 2013-05-24 | 2018-01-09 | Quantapore, Inc. | Nanopore-based nucleic acid analysis with mixed FRET detection |
JP2014235043A (ja) * | 2013-05-31 | 2014-12-15 | キヤノン株式会社 | 検査器具、検査システム、および検査方法 |
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WO2015081294A2 (en) | 2013-11-26 | 2015-06-04 | Stc.Unm | Innovative nanopore sequencing technology |
US10612084B2 (en) * | 2014-04-08 | 2020-04-07 | International Business Machines Corporation | Reduction of entropic barrier of polyelectrolyte molecules in a nanopore device with agarose gel |
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EP2995934A1 (en) | 2014-09-15 | 2016-03-16 | Base4 Innovation Ltd | Improved nanopore plasmonic analyser |
US9885079B2 (en) | 2014-10-10 | 2018-02-06 | Quantapore, Inc. | Nanopore-based polymer analysis with mutually-quenching fluorescent labels |
AU2015335616B2 (en) * | 2014-10-24 | 2019-09-12 | Quantapore, Inc. | Efficient optical analysis of polymers using arrays of nanostructures |
KR20170106487A (ko) | 2015-02-02 | 2017-09-20 | 투 포어 가이즈, 인코포레이티드 | 시료 배경으로부터의 표적 폴리뉴클레오티드의 나노포어 검출 |
WO2016182811A1 (en) | 2015-05-11 | 2016-11-17 | The University Of North Carolina At Chapel Hill | Fluidic devices with nanoscale manifolds for molecular transport, related systems and methods of analysis |
JP6559187B2 (ja) * | 2016-07-01 | 2019-08-14 | ツィンファ ユニバーシティ | 単一分子検出用のキャリア及び単一分子検出装置 |
CN107561053B (zh) * | 2016-07-01 | 2020-04-28 | 清华大学 | 一种单分子检测方法 |
US10823721B2 (en) | 2016-07-05 | 2020-11-03 | Quantapore, Inc. | Optically based nanopore sequencing |
US11331018B2 (en) | 2016-12-22 | 2022-05-17 | Profusa, Inc. | System and single-channel biosensor for and method of determining analyte value |
SG11201910864UA (en) | 2017-06-20 | 2020-01-30 | Illumina Inc | Nanopore sequencers |
JP7237388B2 (ja) * | 2019-12-03 | 2023-03-13 | 国立研究開発法人科学技術振興機構 | ナノポア構造体、ナノポア構造体を含む塩基配列解析装置 |
EP4284751A4 (en) | 2021-01-29 | 2025-01-08 | Armonica Tech Inc | ENHANCEMENT STRUCTURES FOR SURFACE-ENHANCED RAMAN SCATTERING |
Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN1038499A (zh) * | 1988-06-05 | 1990-01-03 | 青州水泵厂 | 往复运动轴杆密封装置 |
US20030017579A1 (en) * | 2001-07-10 | 2003-01-23 | Corn Robert M. | Surface plasmon resonance imaging of micro-arrays |
CN1918467A (zh) * | 2004-02-13 | 2007-02-21 | 欧姆龙株式会社 | 表面等离子共振传感器 |
CN100999764A (zh) * | 2006-12-14 | 2007-07-18 | 上海交通大学 | 光学镊控制单链核酸穿孔速度的方法 |
CN101842692A (zh) * | 2007-09-04 | 2010-09-22 | 贝斯4创新公司 | 设备和方法 |
Family Cites Families (31)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS63273651A (ja) * | 1987-04-30 | 1988-11-10 | Toa Nenryo Kogyo Kk | 超高分子量ポリエチレン微多孔膜の製造方法 |
US6267872B1 (en) * | 1998-11-06 | 2001-07-31 | The Regents Of The University Of California | Miniature support for thin films containing single channels or nanopores and methods for using same |
US6818907B2 (en) * | 2000-10-17 | 2004-11-16 | The President And Fellows Of Harvard College | Surface plasmon enhanced illumination system |
EP1372828A4 (en) * | 2001-03-24 | 2008-10-29 | Aviva Biosciences Corp | BIOCHIPS WITH ION TRANSPORTATION STRUCTURES AND USE METHOD |
US6972173B2 (en) * | 2002-03-14 | 2005-12-06 | Intel Corporation | Methods to increase nucleotide signals by raman scattering |
US7238477B2 (en) * | 2001-09-24 | 2007-07-03 | Intel Corporation | Methods to increase nucleotide signals by Raman scattering |
US6982165B2 (en) * | 2001-09-24 | 2006-01-03 | Intel Corporation | Nucleic acid sequencing by raman monitoring of molecular deconstruction |
DE10157070B4 (de) * | 2001-11-16 | 2005-11-17 | Technische Universität Dresden | Anordnung zur Messung von durch Ionenkanäle fließenden Ionenströmen, sowie Verfahren zur Herstellung dieser und Messverfahren |
US7744816B2 (en) * | 2002-05-01 | 2010-06-29 | Intel Corporation | Methods and device for biomolecule characterization |
US20050019784A1 (en) * | 2002-05-20 | 2005-01-27 | Xing Su | Method and apparatus for nucleic acid sequencing and identification |
US6970239B2 (en) * | 2002-06-12 | 2005-11-29 | Intel Corporation | Metal coated nanocrystalline silicon as an active surface enhanced Raman spectroscopy (SERS) substrate |
ATE479775T1 (de) * | 2002-11-26 | 2010-09-15 | Univ Maryland Biotechnology | Hochempfindliche assays für die pathogendetektion unterverwendung der metallverstärkten fluoreszenz |
JP4173375B2 (ja) * | 2003-01-09 | 2008-10-29 | 三菱レイヨン株式会社 | 電気泳動装置及びそれを用いた生体関連物質の検出方法 |
US7151598B2 (en) * | 2003-04-04 | 2006-12-19 | Vladimir Poponin | Method and apparatus for enhanced nano-spectroscopic scanning |
US7248771B2 (en) * | 2003-06-16 | 2007-07-24 | Brigham Young University | Integrated sensor with electrical and optical single molecule sensitivity |
US20050023156A1 (en) * | 2003-07-30 | 2005-02-03 | Ramsey J. Michael | Nanostructured material transport devices and their fabrication by application of molecular coatings to nanoscale channels |
FR2863053B1 (fr) * | 2003-11-28 | 2007-04-06 | Univ Claude Bernard Lyon | Nouvelles sondes hybrides a luminescence exaltee |
DE10361927B4 (de) * | 2003-12-19 | 2006-12-14 | Technische Universität Dresden | Ionenkanal-Sensorarray |
US20050148064A1 (en) * | 2003-12-29 | 2005-07-07 | Intel Corporation | Microfluid molecular-flow fractionator and bioreactor with integrated active/passive diffusion barrier |
JP4579593B2 (ja) * | 2004-03-05 | 2010-11-10 | キヤノン株式会社 | 標的物質認識素子、検出方法及び装置 |
CN100516835C (zh) * | 2004-03-31 | 2009-07-22 | 欧姆龙株式会社 | 局域等离子共振传感器和检查装置 |
WO2006137891A2 (en) * | 2004-09-29 | 2006-12-28 | University Of Florida Research Foundation, Inc. | Membrane with nanochannels for detection of molecules |
US7289203B2 (en) * | 2004-09-30 | 2007-10-30 | Chromaplex, Inc. | Method and system for spectral analysis of biological materials using stimulated cars |
WO2006050257A2 (en) | 2004-10-29 | 2006-05-11 | Massachusetts Institute Of Tecchnology | Detection of ion channel or receptor activity |
US7476787B2 (en) * | 2005-02-23 | 2009-01-13 | Stc.Unm | Addressable field enhancement microscopy |
US20080245971A1 (en) * | 2005-10-03 | 2008-10-09 | Koninklijke Philips Electronics, N.V. | Biosensors with Improved Sensitivity |
TWI296044B (en) * | 2005-11-03 | 2008-04-21 | Ind Tech Res Inst | Coupled waveguide-surface plasmon resonance biosensor |
US7724373B2 (en) * | 2005-12-16 | 2010-05-25 | Indiana University Research & Technology Corporation | Sub-micron surface plasmon resonance sensor systems |
US7659977B2 (en) * | 2006-04-21 | 2010-02-09 | Intel Corporation | Apparatus and method for imaging with surface enhanced coherent anti-stokes raman scattering (SECARS) |
JP5397577B2 (ja) * | 2007-03-05 | 2014-01-22 | オムロン株式会社 | 表面プラズモン共鳴センサ及び当該センサ用チップ |
US20080239307A1 (en) * | 2007-03-30 | 2008-10-02 | The Regents Of The University Of California | Sequencing single molecules using surface-enhanced Raman scattering |
-
2007
- 2007-09-04 GB GBGB0717150.7A patent/GB0717150D0/en not_active Ceased
-
2008
- 2008-09-04 CN CN2008801144811A patent/CN101842692B/zh not_active Expired - Fee Related
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- 2008-09-04 ES ES08788753.5T patent/ES2617089T3/es active Active
- 2008-09-04 WO PCT/GB2008/050783 patent/WO2009030953A1/en active Application Filing
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- 2014-03-12 JP JP2014048399A patent/JP5827360B2/ja not_active Expired - Fee Related
- 2014-09-19 US US14/490,935 patent/US20150001085A1/en not_active Abandoned
Patent Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN1038499A (zh) * | 1988-06-05 | 1990-01-03 | 青州水泵厂 | 往复运动轴杆密封装置 |
US20030017579A1 (en) * | 2001-07-10 | 2003-01-23 | Corn Robert M. | Surface plasmon resonance imaging of micro-arrays |
CN1918467A (zh) * | 2004-02-13 | 2007-02-21 | 欧姆龙株式会社 | 表面等离子共振传感器 |
CN100999764A (zh) * | 2006-12-14 | 2007-07-18 | 上海交通大学 | 光学镊控制单链核酸穿孔速度的方法 |
CN101842692A (zh) * | 2007-09-04 | 2010-09-22 | 贝斯4创新公司 | 设备和方法 |
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN109387651A (zh) * | 2017-08-08 | 2019-02-26 | 豪夫迈·罗氏有限公司 | 实验室仪器基板 |
CN109387651B (zh) * | 2017-08-08 | 2023-07-28 | 豪夫迈·罗氏有限公司 | 实验室仪器基板 |
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US20110036994A1 (en) | 2011-02-17 |
EP2188618B1 (en) | 2016-10-26 |
WO2009030953A1 (en) | 2009-03-12 |
ES2617089T3 (es) | 2017-06-15 |
JP5827360B2 (ja) | 2015-12-02 |
US20120237940A1 (en) | 2012-09-20 |
US20150001085A1 (en) | 2015-01-01 |
US8865455B2 (en) | 2014-10-21 |
BRPI0816426A2 (pt) | 2015-03-03 |
CN101842692B (zh) | 2012-09-12 |
JP2010538290A (ja) | 2010-12-09 |
CN101842692A (zh) | 2010-09-22 |
CA2698876A1 (en) | 2009-03-12 |
US8440403B2 (en) | 2013-05-14 |
JP2014145771A (ja) | 2014-08-14 |
GB0717150D0 (en) | 2007-10-17 |
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