CN103080317B - 突变型hpgg基序和hdash基序δ‑5去饱和酶以及它们在制备多不饱和脂肪酸中的用途 - Google Patents

突变型hpgg基序和hdash基序δ‑5去饱和酶以及它们在制备多不饱和脂肪酸中的用途 Download PDF

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Publication number
CN103080317B
CN103080317B CN201180040815.7A CN201180040815A CN103080317B CN 103080317 B CN103080317 B CN 103080317B CN 201180040815 A CN201180040815 A CN 201180040815A CN 103080317 B CN103080317 B CN 103080317B
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seq
desaturase
delta
gene
mutant
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Expired - Fee Related
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CN201180040815.7A
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Chinese (zh)
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CN103080317A (zh
Inventor
M.W.博斯蒂克
M.D.多伯特
H.何
S-P.洪
D.M.W.波拉克
P.L.夏普
Y.J.王
Z.薛
N.S.亚达夫
H.张
Q.朱
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EIDP Inc
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EI Du Pont de Nemours and Co
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/001—Oxidoreductases (1.) acting on the CH-CH group of donors (1.3)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6436—Fatty acid esters
    • C12P7/6445—Glycerides
    • C12P7/6472—Glycerides containing polyunsaturated fatty acid [PUFA] residues, i.e. having two or more double bonds in their backbone
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y103/00—Oxidoreductases acting on the CH-CH group of donors (1.3)
    • C12Y103/01—Oxidoreductases acting on the CH-CH group of donors (1.3) with NAD+ or NADP+ as acceptor (1.3.1)
    • C12Y103/01035—Phosphatidylcholine desaturase (1.3.1.35)

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
CN201180040815.7A 2010-08-26 2011-08-26 突变型hpgg基序和hdash基序δ‑5去饱和酶以及它们在制备多不饱和脂肪酸中的用途 Expired - Fee Related CN103080317B (zh)

Applications Claiming Priority (7)

Application Number Priority Date Filing Date Title
US37724810P 2010-08-26 2010-08-26
US61/377248 2010-08-26
US61/377,248 2010-08-26
US201061428277P 2010-12-30 2010-12-30
US61/428,277 2010-12-30
US61/428277 2010-12-30
PCT/US2011/049403 WO2012027698A1 (en) 2010-08-26 2011-08-26 Mutant hpgg motif and hdash motif delta-5 desaturases and their use in making polyunsaturated fatty acids

Publications (2)

Publication Number Publication Date
CN103080317A CN103080317A (zh) 2013-05-01
CN103080317B true CN103080317B (zh) 2017-07-11

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CN201180040815.7A Expired - Fee Related CN103080317B (zh) 2010-08-26 2011-08-26 突变型hpgg基序和hdash基序δ‑5去饱和酶以及它们在制备多不饱和脂肪酸中的用途

Country Status (9)

Country Link
US (1) US8658413B2 (enExample)
EP (1) EP2609202A1 (enExample)
JP (1) JP2013535988A (enExample)
CN (1) CN103080317B (enExample)
AU (1) AU2011293189B2 (enExample)
BR (1) BR112013004355A2 (enExample)
CA (1) CA2808002A1 (enExample)
CL (1) CL2013000534A1 (enExample)
WO (1) WO2012027698A1 (enExample)

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WO2012138613A1 (en) * 2011-04-05 2012-10-11 E. I. Du Pont De Nemours And Company Yarrowia n-alkane-hydroxylating cytochrome p450 promoter regions for gene expression in yeast
US20150089689A1 (en) 2012-01-23 2015-03-26 E I Du Pont Nemours And Company Down-regulation of gene expression using artificial micrornas for silencing fatty acid biosynthetic genes
CN102978177B (zh) * 2012-11-09 2015-06-03 浙江工业大学 冬虫夏草合成代谢二十碳五烯酸的△-5-脱氢酶、基因及应用
EP4049537A1 (en) 2018-12-12 2022-08-31 Nippon Suisan Kaisha, Ltd. A composition containing highly unsaturated fatty acid or alkyl ester thereof and a method for producing the same

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WO2010033753A2 (en) * 2008-09-19 2010-03-25 E. I. Du Pont De Nemours And Company Mutant delta5 desaturases and their use in making polyunsaturated fatty acids

Also Published As

Publication number Publication date
AU2011293189B2 (en) 2017-02-16
US20120052549A1 (en) 2012-03-01
BR112013004355A2 (pt) 2021-05-04
US8658413B2 (en) 2014-02-25
CL2013000534A1 (es) 2014-02-07
WO2012027698A1 (en) 2012-03-01
CA2808002A1 (en) 2012-03-01
CN103080317A (zh) 2013-05-01
AU2011293189A1 (en) 2013-01-10
EP2609202A1 (en) 2013-07-03
JP2013535988A (ja) 2013-09-19

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