CL2013000534A1 - Polipeptido mutante que tiene actividad delta-5 desaturasa; molecula de acido nucleico aislada que lo codifica; célula huesped transformada que expresa el polipeptido. - Google Patents

Polipeptido mutante que tiene actividad delta-5 desaturasa; molecula de acido nucleico aislada que lo codifica; célula huesped transformada que expresa el polipeptido.

Info

Publication number
CL2013000534A1
CL2013000534A1 CL2013000534A CL2013000534A CL2013000534A1 CL 2013000534 A1 CL2013000534 A1 CL 2013000534A1 CL 2013000534 A CL2013000534 A CL 2013000534A CL 2013000534 A CL2013000534 A CL 2013000534A CL 2013000534 A1 CL2013000534 A1 CL 2013000534A1
Authority
CL
Chile
Prior art keywords
polypeptide
encodes
delta
nucleic acid
host cell
Prior art date
Application number
CL2013000534A
Other languages
English (en)
Spanish (es)
Inventor
Qun Zhu
Michael W Bostick
Melissa D Daubert
Hongxiang He
Seung-Pyo Hong
Dana M Walters Pollak
Pamela L Sharpe
Zhixiong Xue
Narendra S Yadav
Hongxiang Zhang
Yehong Jamie Wang
Original Assignee
Du Pont
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Du Pont filed Critical Du Pont
Publication of CL2013000534A1 publication Critical patent/CL2013000534A1/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/001—Oxidoreductases (1.) acting on the CH-CH group of donors (1.3)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6436—Fatty acid esters
    • C12P7/6445—Glycerides
    • C12P7/6472—Glycerides containing polyunsaturated fatty acid [PUFA] residues, i.e. having two or more double bonds in their backbone
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y103/00—Oxidoreductases acting on the CH-CH group of donors (1.3)
    • C12Y103/01—Oxidoreductases acting on the CH-CH group of donors (1.3) with NAD+ or NADP+ as acceptor (1.3.1)
    • C12Y103/01035—Phosphatidylcholine desaturase (1.3.1.35)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
CL2013000534A 2010-08-26 2013-02-25 Polipeptido mutante que tiene actividad delta-5 desaturasa; molecula de acido nucleico aislada que lo codifica; célula huesped transformada que expresa el polipeptido. CL2013000534A1 (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US37724810P 2010-08-26 2010-08-26
US201061428277P 2010-12-30 2010-12-30

Publications (1)

Publication Number Publication Date
CL2013000534A1 true CL2013000534A1 (es) 2014-02-07

Family

ID=45697767

Family Applications (1)

Application Number Title Priority Date Filing Date
CL2013000534A CL2013000534A1 (es) 2010-08-26 2013-02-25 Polipeptido mutante que tiene actividad delta-5 desaturasa; molecula de acido nucleico aislada que lo codifica; célula huesped transformada que expresa el polipeptido.

Country Status (9)

Country Link
US (1) US8658413B2 (enExample)
EP (1) EP2609202A1 (enExample)
JP (1) JP2013535988A (enExample)
CN (1) CN103080317B (enExample)
AU (1) AU2011293189B2 (enExample)
BR (1) BR112013004355A2 (enExample)
CA (1) CA2808002A1 (enExample)
CL (1) CL2013000534A1 (enExample)
WO (1) WO2012027698A1 (enExample)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2012138613A1 (en) * 2011-04-05 2012-10-11 E. I. Du Pont De Nemours And Company Yarrowia n-alkane-hydroxylating cytochrome p450 promoter regions for gene expression in yeast
WO2013112523A2 (en) * 2012-01-23 2013-08-01 E. I. Du Pont De Nemours And Company Down-regulation of gene expression using artificial micrornas for silencing fatty acid biosynthestic genes
CN102978177B (zh) * 2012-11-09 2015-06-03 浙江工业大学 冬虫夏草合成代谢二十碳五烯酸的△-5-脱氢酶、基因及应用
EP3666082B1 (en) 2018-12-12 2022-05-04 Nippon Suisan Kaisha, Ltd. A composition containing highly unsaturated fatty acid or alkyl ester thereof and a method for producing the same

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US7879591B2 (en) 2004-11-04 2011-02-01 E.I. Du Pont De Nemours And Company High eicosapentaenoic acid producing strains of Yarrowia lipolytica
CN103589650A (zh) 2005-03-18 2014-02-19 米克罗比亚公司 产油酵母和真菌中类胡萝卜素的产生
US7470532B2 (en) 2005-10-19 2008-12-30 E.I. Du Pont De Nemours And Company Mortierella alpina C16/18 fatty acid elongase
WO2007061742A1 (en) 2005-11-23 2007-05-31 E. I. Du Pont De Nemours And Company Delta-9 elongases and their use in making polyunsaturated fatty acids
US7695950B2 (en) 2006-05-17 2010-04-13 E. I. Du Pont De Nemours And Company Δ5 desaturase and its use in making polyunsaturated fatty acids
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US7709239B2 (en) 2006-12-07 2010-05-04 E.I. Du Pont De Nemours And Company Mutant Δ8 desaturase genes engineered by targeted mutagenesis and their use in making polyunsaturated fatty acids
US8846374B2 (en) 2006-12-12 2014-09-30 E I Du Pont De Nemours And Company Carotenoid production in a recombinant oleaginous yeast
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DK2129777T3 (en) 2007-04-03 2016-01-25 Du Pont MULTIZYMER AND USE THEREOF FOR THE PRODUCTION OF polyunsaturated fatty acids
US8188338B2 (en) 2007-04-10 2012-05-29 E. I. Du Pont De Nemours And Company Delta-8 desaturases and their use in making polyunsaturated fatty acids
US8119860B2 (en) 2007-04-16 2012-02-21 E. I. Du Pont De Nemours And Company Delta-9 elongases and their use in making polyunsaturated fatty acids
US7943365B2 (en) 2007-05-03 2011-05-17 E.I. Du Pont De Nemours And Company Δ-5 desaturases and their use in making polyunsaturated fatty acids
US8323935B2 (en) 2007-10-03 2012-12-04 E I Du Pont De Nemours And Company Optimized strains of Yarrowia lipolytica for high eicosapentaenoic acid production
DK2324119T3 (en) * 2008-09-19 2016-07-18 Du Pont Mutant DELTA5 Desaturases AND USE THEREOF FOR THE PRODUCTION OF polyunsaturated fatty acids
CA2744716C (en) 2008-12-18 2017-05-23 E.I. Du Pont De Nemours And Company Reducing byproduction of malonates in a fermentation process
US8399226B2 (en) 2009-06-16 2013-03-19 E I Du Pont De Nemours And Company High eicosapentaenoic acid oils from improved optimized strains of Yarrowia lipolytica
US8703473B2 (en) * 2010-08-26 2014-04-22 E I Du Pont De Nemours And Company Recombinant microbial host cells for high eicosapentaenoic acid production

Also Published As

Publication number Publication date
JP2013535988A (ja) 2013-09-19
AU2011293189A1 (en) 2013-01-10
WO2012027698A1 (en) 2012-03-01
CA2808002A1 (en) 2012-03-01
BR112013004355A2 (pt) 2021-05-04
US20120052549A1 (en) 2012-03-01
AU2011293189B2 (en) 2017-02-16
CN103080317A (zh) 2013-05-01
US8658413B2 (en) 2014-02-25
CN103080317B (zh) 2017-07-11
EP2609202A1 (en) 2013-07-03

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