CA2808002A1 - Mutant hpgg motif and hdash motif delta-5 desaturases and their use in making polyunsaturated fatty acids - Google Patents

Mutant hpgg motif and hdash motif delta-5 desaturases and their use in making polyunsaturated fatty acids Download PDF

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Publication number
CA2808002A1
CA2808002A1 CA2808002A CA2808002A CA2808002A1 CA 2808002 A1 CA2808002 A1 CA 2808002A1 CA 2808002 A CA2808002 A CA 2808002A CA 2808002 A CA2808002 A CA 2808002A CA 2808002 A1 CA2808002 A1 CA 2808002A1
Authority
CA
Canada
Prior art keywords
seq
delta
egd5r
desaturase
motif
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA2808002A
Other languages
English (en)
French (fr)
Inventor
Michael W. Bostick
Melissa D. Daubert
Hongxian He
Seung-Pyo Hong
Dana M. Walters Pollak
Pamela L. Sharpe
Yehong Jamie Wang
Zhixiong Xue
Narendra S. Yadav
Hongxiang Zhang
Qun Zhu
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
EIDP Inc
Original Assignee
EI Du Pont de Nemours and Co
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by EI Du Pont de Nemours and Co filed Critical EI Du Pont de Nemours and Co
Publication of CA2808002A1 publication Critical patent/CA2808002A1/en
Abandoned legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/001—Oxidoreductases (1.) acting on the CH-CH group of donors (1.3)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6436—Fatty acid esters
    • C12P7/6445—Glycerides
    • C12P7/6472—Glycerides containing polyunsaturated fatty acid [PUFA] residues, i.e. having two or more double bonds in their backbone
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y103/00—Oxidoreductases acting on the CH-CH group of donors (1.3)
    • C12Y103/01—Oxidoreductases acting on the CH-CH group of donors (1.3) with NAD+ or NADP+ as acceptor (1.3.1)
    • C12Y103/01035—Phosphatidylcholine desaturase (1.3.1.35)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
CA2808002A 2010-08-26 2011-08-26 Mutant hpgg motif and hdash motif delta-5 desaturases and their use in making polyunsaturated fatty acids Abandoned CA2808002A1 (en)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US37724810P 2010-08-26 2010-08-26
US61/377,248 2010-08-26
US201061428277P 2010-12-30 2010-12-30
US61/428,277 2010-12-30
PCT/US2011/049403 WO2012027698A1 (en) 2010-08-26 2011-08-26 Mutant hpgg motif and hdash motif delta-5 desaturases and their use in making polyunsaturated fatty acids

Publications (1)

Publication Number Publication Date
CA2808002A1 true CA2808002A1 (en) 2012-03-01

Family

ID=45697767

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2808002A Abandoned CA2808002A1 (en) 2010-08-26 2011-08-26 Mutant hpgg motif and hdash motif delta-5 desaturases and their use in making polyunsaturated fatty acids

Country Status (9)

Country Link
US (1) US8658413B2 (enExample)
EP (1) EP2609202A1 (enExample)
JP (1) JP2013535988A (enExample)
CN (1) CN103080317B (enExample)
AU (1) AU2011293189B2 (enExample)
BR (1) BR112013004355A2 (enExample)
CA (1) CA2808002A1 (enExample)
CL (1) CL2013000534A1 (enExample)
WO (1) WO2012027698A1 (enExample)

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Publication number Priority date Publication date Assignee Title
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BR112014018009A2 (pt) 2012-01-23 2018-06-26 Du Pont sequência de ácido nucleico isolada, construção recombinante, célula vegetal, método para reduzir a expressão de pelo menos um gene biossintético de ácido graxo vegetal, método para reduzir a expressão de dois ou mais genes biossintéticos de ácido graxo vegetal e planta ou semente transgênica
CN102978177B (zh) * 2012-11-09 2015-06-03 浙江工业大学 冬虫夏草合成代谢二十碳五烯酸的△-5-脱氢酶、基因及应用
EP3666082B1 (en) 2018-12-12 2022-05-04 Nippon Suisan Kaisha, Ltd. A composition containing highly unsaturated fatty acid or alkyl ester thereof and a method for producing the same

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Also Published As

Publication number Publication date
US20120052549A1 (en) 2012-03-01
AU2011293189B2 (en) 2017-02-16
JP2013535988A (ja) 2013-09-19
BR112013004355A2 (pt) 2021-05-04
AU2011293189A1 (en) 2013-01-10
US8658413B2 (en) 2014-02-25
WO2012027698A1 (en) 2012-03-01
CN103080317A (zh) 2013-05-01
CL2013000534A1 (es) 2014-02-07
CN103080317B (zh) 2017-07-11
EP2609202A1 (en) 2013-07-03

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Legal Events

Date Code Title Description
EEER Examination request

Effective date: 20160822

FZDE Discontinued

Effective date: 20200831