CN103040924A - Radix millettiae speciosae extract and application thereof in preparing drugs for treating osteoporosis - Google Patents
Radix millettiae speciosae extract and application thereof in preparing drugs for treating osteoporosis Download PDFInfo
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- CN103040924A CN103040924A CN2013100113452A CN201310011345A CN103040924A CN 103040924 A CN103040924 A CN 103040924A CN 2013100113452 A CN2013100113452 A CN 2013100113452A CN 201310011345 A CN201310011345 A CN 201310011345A CN 103040924 A CN103040924 A CN 103040924A
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Abstract
The invention discloses a radix millettiae speciosae extract and an application of the radix millettiae speciosae extract in preparing drugs for treating osteoporosis. The radix millettiae speciosae extract is obtained by drying the roots of radix millettiae speciosae and crushing into powder, then refluxing with ethanol and extracting, filtering an extracting solution, decompressing and recycling a solvent completely. The radix millettiae speciosae extract is discovered with a good efficacy in treating osteoporosis on a basis of efficacy test, so that the radix millettiae speciosae extract can be used for preparing drugs for treating osteoporosis, and an effective way is provided for the treatment of osteoporosis, and moreover, the preparation method is simple, and the cost is low.
Description
Technical field
The invention belongs to the plant application, relate to a kind of vegetable active extract and application thereof, specifically a kind of Millettia speciosa Champ.extract and the application in preparation treatment medicine for treating osteoporosis thereof.
Background technology
Osteoporosis (osteoporosis, OP) is to increase and the easy a kind of systemic disease that occurs to fracture as feature take the minimizing of bone amount, bone microstructure degeneration, fragility, is mainly in postmenopausal women, old man and multiple patients with chronic diseases.Because the osteoporosis disability rate is higher, greatly affect people's quality of life, bring heavy financial burden for family and society.Along with the population structure aging, osteoporotic problem is more and more outstanding.
Modern medicine thinks that osteoporotic basic pathology mechanism is that bone resorption and osteoplastic coupling have occurred unbalancely in the bone metabolism process, causes the alcium and phosphor metabolization in the human body uneven, and bone density reduces and the clinical symptoms that causes gradually.At present there is no desirable method for osteoporotic treatment clinically.
Though without osteoporotic disease name, study carefully the category that its clinical manifestation, pathogenesis should belong to " rheumatism involving the bone ", " atrophic debility of bones " in the Chinese medicine.Theory of Chinese medical science thinks that osteoporotic etiology and pathogenesis mainly is deficiency of the kidney, insufficiency of the spleen and expectorant stasis blocking arteries and veins.The rule for the treatment of then is invigorating the kidney and strengthening the bones, invigorating the spleen and benefiting QI and promoting blood circulation to remove obstruction in the collateral.
Millettia Speciosa (Millettia specisoa Champ.), hangs upside down Jin Zhong, potato energetically at another name pig's feet large bamboo hat with a conical crown and broad brim, Jin Zhonggen, Radix Millettiae Speciosae, is pulse family Millettia plant, and is upright or hang down loosely undershrub, high 1～2 meter.Root system is directly stretched downwards, is permitted for long 1 meter.Millettia Speciosa is used as medicine with root, through retrieval, mainly be immunoregulation effect to the pharmacological research of Millettia Speciosa (root of Millettia Speciosa) at present, liver protection effect, antiinflammatory action, antioxidation, effect of scavenging radical, antitumor action, eliminate the phlegm, antitussive, antiasthmatic effect and blood coagulation resisting function research, the application in the treatment medicine for treating osteoporosis there is no report.
Summary of the invention
The purpose of this invention is to provide a kind of Millettia speciosa Champ.extract and the application in preparation treatment medicine for treating osteoporosis thereof.
Millettia speciosa Champ.extract of the present invention is with the root oven dry of Millettia Speciosa and is ground into powder, then extracts with alcohol reflux, filters extracting solution, and reclaim under reduced pressure is solvent to the greatest extent, obtains extractum shape Millettia speciosa Champ.extract.
A kind of Millettia Speciosa polar fraction extract, to get above-mentioned Millettia speciosa Champ.extract thin up, extract successively with petroleum ether, chloroform and ethyl acetate in order, remaining aqueous solution after acetic acid ethyl acetate extract or the extraction is evaporated to the extractum shape, and the powder that vacuum drying obtains is Millettia Speciosa ethyl acetate extract extract, Millettia Speciosa water extractive part.
The present invention also comprises above-mentioned Millettia speciosa Champ.extract, Millettia Speciosa ethyl acetate extract extract, the application of Millettia Speciosa water extractive part in preparation treatment medicine for treating osteoporosis.The inventor finds that the said extracted thing has good drug effect in the treatment osteoporosis, can be used for preparing the treatment medicine for treating osteoporosis on test of pesticide effectiveness basis.
Described treatment medicine for treating osteoporosis is that Millettia speciosa Champ.extract is cooperated with conventional adjuvant, uses preparation in the clinically permission that the existing technique of employing makes.
Described conventional adjuvant is solvent, disintegrating agent, correctives, antiseptic, coloring agent, binding agent, lubricant, wetting agent, thickening agent, solubilizing agent or substrate.
Described interior be powder, granule, tablet, capsule, pill, oral liquid, dispersible tablet, spray, intravenous injection, intramuscular dose, inhalant or gel with preparation.
The present invention also comprises one or more the ingredients combination drug in said extracted thing and medicine for rheumatism, damp-clearing drug, drug for invigorating blood circulation and eliminating stasis, the qi-restoratives medicine.
Described medicine for rheumatism is Fructus Chaenomelis, Radix Gentianae Macrophyllae, Cortex Acanthopancis, Herba Taxilli, Rhizoma Cibotii etc.
Described damp-clearing drug is Poria, Rhizoma Alismatis etc.
Described drug for invigorating blood circulation and eliminating stasis is Rhizoma Chuanxiong, Rhizoma Corydalis, Radix Achyranthis Bidentatae, Rhizoma Drynariae etc.
Described qi-restoratives medicine is yang-tonifying drug, YIN-tonifying drug, Qi-tonifying drug or hematonic.Described yang-tonifying drug comprises Herba Epimedii, the Cortex Eucommiae, Radix Dipsaci, Colla cornus cervi, Semen Cuscutae, Fructus Psoraleae, Rhizoma Curculiginis, Herba Cistanches, Placenta Hominis, Radix Morindae Officinalis, Cornu Cervi Pantotrichum, Semen Juglandis, Semen Trigonellae etc.; Described YIN-tonifying drug comprises Radix Ophiopogonis, Rhizoma Polygonati, Fructus Lycii, Fructus Ligustri Lucidi, Herba Ecliptae, Carapax et Plastrum Testudinis, Carapax Trionycis, Herba Dendrobii, Rhizoma Polygonati Odorati, Radix Asparagi etc.; Described Qi-tonifying drug comprises the Radix Astragali, Rhizoma Dioscoreae, Radix Glycyrrhizae, the Rhizoma Atractylodis Macrocephalae, Radix Codonopsis, Radix Ginseng, Fructus Jujubae; Described hematonic comprises Radix Rehmanniae Preparata, Radix Angelicae Sinensis, Radix Polygoni Multiflori, the Radix Paeoniae Alba, Colla Corii Asini etc.
Millettia speciosa Champ.extract provided by the present invention has good drug effect in the treatment osteoporosis, can be used for developing the treatment medicine for treating osteoporosis, avoid the large shortcoming of chemical synthetic drug side effect, for osteoporotic treatment provides effective way, its preparation method is simple, and cost is low.
The specific embodiment
Below in conjunction with embodiment, the specific embodiment of the present invention is described in further detail.Following examples are used for explanation the present invention, but are not used for limiting the scope of the invention.The experimental technique of unreceipted actual conditions in the following example, usually according to normal condition, or the condition of advising according to manufacturer.
Embodiment one
Powder is dried and be ground into to the root of Millettia Speciosa, powder extracts 3 times with 95% methanol eddy, merge extractive liquid,, filter, reclaim under reduced pressure to the greatest extent methanol obtains extractum shape methanol crude extract, and then the methanol crude extract thin up uses petroleum ether, chloroform, ethyl acetate extraction successively, respectively extract is evaporated to the extractum shape, the powder that vacuum drying obtains is respectively Millettia Speciosa petroleum ether part extract, chloroform extract extract, ethyl acetate extract extract.Remaining aqueous solution is evaporated to the extractum shape after the extraction, and the powder that vacuum drying obtains is Millettia Speciosa water extractive part.
Embodiment two
Powder is dried and be ground into to the root of Millettia Speciosa, powder is with 95% alcohol reflux 4 times, merge extractive liquid,, filter, reclaim under reduced pressure to the greatest extent ethanol obtains extractum shape alcohol extracts, and then the alcohol extracts thin up uses petroleum ether, chloroform, ethyl acetate extraction successively, respectively extract is evaporated to the extractum shape, the powder that vacuum drying obtains is respectively Millettia Speciosa petroleum ether part extract, chloroform extract extract, ethyl acetate extract extract.Remaining aqueous solution is evaporated to the extractum shape after the extraction, and the powder that vacuum drying obtains is Millettia Speciosa water extractive part.
Embodiment three
Powder is dried and be ground into to the root of Millettia Speciosa, powder is with 75% alcohol reflux 6 times, merge extractive liquid,, filter, reclaim under reduced pressure to the greatest extent ethanol obtains extractum shape methanol crude extract, and then the alcohol extracts thin up uses petroleum ether, chloroform, ethyl acetate extraction successively, respectively extract is evaporated to the extractum shape, the powder that vacuum drying obtains is respectively Millettia Speciosa petroleum ether part extract, chloroform extract extract, ethyl acetate extract extract.Remaining aqueous solution is evaporated to the extractum shape after the extraction, and the powder that vacuum drying obtains is Millettia Speciosa water extractive part.
Experiment:
One, in vitro tests
The extract (pure crude extract, petroleum ether part extract, chloroform extract extract, ethyl acetate extract extract, water extractive part) that embodiment obtains is used respectively the PBS(phosphate buffer) be mixed with the 10mg/mL storing solution, be diluted to before use 1000 μ g/mL, 100 μ g/mL.
Experimental procedure: get the Mus osteoblast (OB) of former culture with 2 * 10
4Individual/mL is inoculated in 96 orifice plates, and every pore volume 90 μ L are at CO
2Cultivate 24h in the incubator.Behind cell attachment, the PBS solution 10 μ L that add extract, the final concentration that makes extract is 100 μ g/mL, 10 μ g/mLL, behind the cultivation 48h, every hole adds MTT solution (5 mg/mL) 10 μ L, 4h is hatched in continuation in incubator, incline behind the supernatant, every hole adds DMSO100 μ L, measures each hole absorbance in the 570nm place with microplate reader after the vibration.Set simultaneously blank group (replacing extract with the PBS with volume), calculate cell survival rate (Cell viability)=(extract group OD value/blank group OD value) * 100% according to following formula.
Acquired results sees Table 1.
Each polar fraction of table 1 Millettia Speciosa is on the impact of osteoblastic proliferation
Annotate: 1, compare * P＜0.05 with matched group, * * P＜0.01, * * * P＜0.001,
Experimental result shows that the ethyl acetate extract of Millettia Speciosa and water extractive part all have obvious proliferation to osteoblast under 100 μ g/mL and 10 μ g/mL concentration conditions.
Two, pharmacodynamics EXPERIMENTAL EXAMPLE in the Millettia Speciosa alcohol crude extract body
1. experimental technique:
1.1 dosage design and grouping: the dosage of three experimental grouies is respectively 800,600,200mg/kgBW; Other establishes the contrast of castration model, estradiol positive control and sham-operation negative control group.
1. 2 oophorectomys:
At first through the anesthesia of rats by intraperitoneal injection 50mg/kgBW pentobarbital sodium solution, after middle hypogastric region unhairing is carried out partly sterilised with iodine tincture sense and ethanol, do 1cm left and right sides otch along the stomach wall median line rat is implemented the castration operation, extract bilateral ovaries.It is the same but do not extract bilateral ovaries, the operating comparison of playing tricks that other gets one group of rat operation.
1.3 animal grouping:
The animal of will performing the operation is divided into three experimental grouies and two matched groups at random according to body weight, i.e. high dose group, middle dosage group, low dose group, the contrast of castration model, estradiol positive control, and other establishes the sham-operation negative control, and every group of single cage of 10 animals raised.Three experimental grouies give tested material by design flow with 1ml/100gBW per os gavage, and sham-operation negative control and the contrast of castration model are with equivalent deionized water gavage; It is 2 mg/kgBW that the estradiol positive control gives dosage.The all experiments were animal is all drunk deionized water, weighs weekly once, and be 12 weeks experimental period.
1.4 measure:
Animal feeding sacrificed by decapitation after 12 weeks is used the SD-1000 borne densitometers, measures the bone density of rat femur mid point and femur distal end.
2. Millettia Speciosa alcohol crude extract is on the impact of rat bone density:
Experimental result shows, with model control group relatively, the middle and high dosage group of Millettia Speciosa alcohol crude extract be significantly increased rat femur distal end bone density value and bone mid point bone density value (p＜0.05).
The above only is preferred implementation of the present invention; should be pointed out that for those skilled in the art, under the prerequisite that does not break away from the technology of the present invention principle; can also make some improvements and modifications, these improvements and modifications also should be considered as protection scope of the present invention.
Claims (7)
1. a Millettia speciosa Champ.extract is characterized in that powder is dried and be ground into to the root of Millettia Speciosa, then extracts with alcohol reflux, filters extracting solution, and reclaim under reduced pressure is solvent to the greatest extent, obtains extractum shape Millettia speciosa Champ.extract.
2. Millettia Speciosa polar fraction extract, it is characterized in that: get Millettia speciosa Champ.extract thin up claimed in claim 1, extract successively with petroleum ether, chloroform and ethyl acetate in order, remaining aqueous solution after acetic acid ethyl acetate extract or the extraction is evaporated to the extractum shape, and the powder that vacuum drying obtains is Millettia Speciosa ethyl acetate extract extract, Millettia Speciosa water extractive part.
3. claim 1 or 2 described Millettia speciosa Champ.extract, Millettia Speciosa ethyl acetate extract extract, the application of Millettia Speciosa water extractive part in preparation treatment medicine for treating osteoporosis.
4. application according to claim 3 is characterized in that: described treatment medicine for treating osteoporosis is that Millettia speciosa Champ.extract is cooperated with conventional adjuvant, uses preparation in the clinically permission that the existing technique of employing makes.
5. application according to claim 4 is characterized in that: described conventional adjuvant is solvent, disintegrating agent, correctives, antiseptic, coloring agent, binding agent, lubricant, wetting agent, thickening agent, solubilizing agent or substrate.
6. application according to claim 4 is characterized in that: described interior be powder, granule, tablet, capsule, pill, oral liquid, dispersible tablet, spray, intravenous injection, intramuscular dose, inhalant or gel with preparation.
7. the ingredients combination drug of one or more in claim 1 or 2 described extracts and medicine for rheumatism, damp-clearing drug, drug for invigorating blood circulation and eliminating stasis, the qi-restoratives medicine.
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Cited By (21)
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|---|---|---|---|---|
| CN103720994A (en) * | 2013-12-25 | 2014-04-16 | 赵鹏 | Chinese medicinal herb formula for relaxing sinews, promoting blood circulation and strengthening bone and preparation method of formula |
| CN103876143A (en) * | 2014-03-31 | 2014-06-25 | 苏志恒 | Sugar-free radix millettiae speciosae buccal tablet and preparation method thereof |
| CN103893390A (en) * | 2014-04-15 | 2014-07-02 | 孟小斐 | Traditional Chinese medicine for preventing osteoporosis after colorectal cancer chemotherapy and preparation method thereof |
| CN104138521A (en) * | 2014-08-05 | 2014-11-12 | 苏义燕 | Traditional Chinese medicine for treating postmenopausal osteoporosis in pattern of syndrome of deficiency of both yin and yang |
| CN104398802A (en) * | 2014-12-05 | 2015-03-11 | 李崇铭 | Traditional Chinese medicine preparation for strengthening spines and invigorating bones as well as preparation method thereof |
| CN104645321A (en) * | 2015-03-05 | 2015-05-27 | 济南方宇文化传媒有限公司 | Sugar pills for promoting skeletal development of children and preparation method of sugar pills |
| CN104740080A (en) * | 2015-03-02 | 2015-07-01 | 赵彦侠 | Traditional Chinese medicine preparation for treating osteoporosis |
| CN104825652A (en) * | 2015-04-28 | 2015-08-12 | 广西大学 | Traditional Chinese medicine preparation for treating osteoporosis |
| CN104877852A (en) * | 2015-04-27 | 2015-09-02 | 吴中区胥口精益生物医药研究所 | Preparation technology of rice wine capable of nourishing yin and tonifying kidneys |
| CN105031124A (en) * | 2015-07-08 | 2015-11-11 | 烟台巨先药业有限公司 | Bone-invigorating pill and preparation method thereof |
| CN105213905A (en) * | 2015-10-19 | 2016-01-06 | 青岛市中心医院 | A kind of Chinese medicine composition and application thereof preventing and treating postmenopausal osteoporosis |
| CN105560980A (en) * | 2015-12-31 | 2016-05-11 | 广西中医药大学 | Zhuang medicine composition for treating perimenopausal syndromes |
| CN105709056A (en) * | 2016-03-25 | 2016-06-29 | 王守彬 | Oral traditional Chinese medicine preparation for anaphase of bone fracture |
| CN105748830A (en) * | 2016-04-26 | 2016-07-13 | 郑州市中医院 | Bone strengthening soup and preparing method thereof |
| CN105768077A (en) * | 2016-03-15 | 2016-07-20 | 桂林亦元生现代生物技术有限公司 | Instant radix millettiae speciosae and preparation method and consumption method thereof |
| CN105832838A (en) * | 2016-06-01 | 2016-08-10 | 朱朝晖 | Traditional Chinese medicine preparation for oral administration for late fracture treatment and recovery |
| CN105920526A (en) * | 2016-05-12 | 2016-09-07 | 中国热带农业科学院热带作物品种资源研究所 | Beautiful millettia root composition capable of adjusting immunity as well as preparation method and applications thereof |
| CN106474269A (en) * | 2016-11-30 | 2017-03-08 | 广西万德药业有限公司 | A kind of Chinese medicine composition that reinforces the kidney to strengthen the bone and preparation method thereof |
| CN106474179A (en) * | 2016-10-14 | 2017-03-08 | 广西放心源生物科技有限公司 | A kind of preparation method of beautiful millettia root microcapsule granule |
| CN108465031A (en) * | 2018-05-03 | 2018-08-31 | 佛山科学技术学院 | A kind of Chinese medicine composition and preparation method thereof for treating osteoporosis |
| CN110917238A (en) * | 2019-12-26 | 2020-03-27 | 广州泽力医药科技有限公司 | Millettia speciosa champ total-component extract and preparation method and application thereof |
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| CN103720994A (en) * | 2013-12-25 | 2014-04-16 | 赵鹏 | Chinese medicinal herb formula for relaxing sinews, promoting blood circulation and strengthening bone and preparation method of formula |
| CN103876143A (en) * | 2014-03-31 | 2014-06-25 | 苏志恒 | Sugar-free radix millettiae speciosae buccal tablet and preparation method thereof |
| CN103893390A (en) * | 2014-04-15 | 2014-07-02 | 孟小斐 | Traditional Chinese medicine for preventing osteoporosis after colorectal cancer chemotherapy and preparation method thereof |
| CN104138521A (en) * | 2014-08-05 | 2014-11-12 | 苏义燕 | Traditional Chinese medicine for treating postmenopausal osteoporosis in pattern of syndrome of deficiency of both yin and yang |
| CN104398802A (en) * | 2014-12-05 | 2015-03-11 | 李崇铭 | Traditional Chinese medicine preparation for strengthening spines and invigorating bones as well as preparation method thereof |
| CN104740080A (en) * | 2015-03-02 | 2015-07-01 | 赵彦侠 | Traditional Chinese medicine preparation for treating osteoporosis |
| CN104645321A (en) * | 2015-03-05 | 2015-05-27 | 济南方宇文化传媒有限公司 | Sugar pills for promoting skeletal development of children and preparation method of sugar pills |
| CN104877852A (en) * | 2015-04-27 | 2015-09-02 | 吴中区胥口精益生物医药研究所 | Preparation technology of rice wine capable of nourishing yin and tonifying kidneys |
| CN104825652A (en) * | 2015-04-28 | 2015-08-12 | 广西大学 | Traditional Chinese medicine preparation for treating osteoporosis |
| CN105031124A (en) * | 2015-07-08 | 2015-11-11 | 烟台巨先药业有限公司 | Bone-invigorating pill and preparation method thereof |
| CN105213905A (en) * | 2015-10-19 | 2016-01-06 | 青岛市中心医院 | A kind of Chinese medicine composition and application thereof preventing and treating postmenopausal osteoporosis |
| CN105560980A (en) * | 2015-12-31 | 2016-05-11 | 广西中医药大学 | Zhuang medicine composition for treating perimenopausal syndromes |
| CN105768077A (en) * | 2016-03-15 | 2016-07-20 | 桂林亦元生现代生物技术有限公司 | Instant radix millettiae speciosae and preparation method and consumption method thereof |
| CN105709056A (en) * | 2016-03-25 | 2016-06-29 | 王守彬 | Oral traditional Chinese medicine preparation for anaphase of bone fracture |
| CN105748830A (en) * | 2016-04-26 | 2016-07-13 | 郑州市中医院 | Bone strengthening soup and preparing method thereof |
| CN105920526A (en) * | 2016-05-12 | 2016-09-07 | 中国热带农业科学院热带作物品种资源研究所 | Beautiful millettia root composition capable of adjusting immunity as well as preparation method and applications thereof |
| CN105920526B (en) * | 2016-05-12 | 2020-02-18 | 中国热带农业科学院热带作物品种资源研究所 | Millettia speciosa champ composition for regulating immunity and preparation method and application thereof |
| CN105832838A (en) * | 2016-06-01 | 2016-08-10 | 朱朝晖 | Traditional Chinese medicine preparation for oral administration for late fracture treatment and recovery |
| CN106474179A (en) * | 2016-10-14 | 2017-03-08 | 广西放心源生物科技有限公司 | A kind of preparation method of beautiful millettia root microcapsule granule |
| CN106474269A (en) * | 2016-11-30 | 2017-03-08 | 广西万德药业有限公司 | A kind of Chinese medicine composition that reinforces the kidney to strengthen the bone and preparation method thereof |
| CN108465031A (en) * | 2018-05-03 | 2018-08-31 | 佛山科学技术学院 | A kind of Chinese medicine composition and preparation method thereof for treating osteoporosis |
| CN110917238A (en) * | 2019-12-26 | 2020-03-27 | 广州泽力医药科技有限公司 | Millettia speciosa champ total-component extract and preparation method and application thereof |
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