CN103039542A - Natural vegetal bee medicine and preparation method thereof - Google Patents
Natural vegetal bee medicine and preparation method thereof Download PDFInfo
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- CN103039542A CN103039542A CN2012105926638A CN201210592663A CN103039542A CN 103039542 A CN103039542 A CN 103039542A CN 2012105926638 A CN2012105926638 A CN 2012105926638A CN 201210592663 A CN201210592663 A CN 201210592663A CN 103039542 A CN103039542 A CN 103039542A
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Abstract
The invention relates to natural vegetal bee medicine and a preparation method thereof. The bee medicine comprises the following components in percentage by weight: 60 percent to 90wt percent of pterocarya stenoptera leaf extract, 5 to 20 percent of tea saponin, 1 to 10 percent of stemona root extract, 0.5 to 9 percent of sweet wormwood and 0.1 to 2 percent of tween-80. The bee medicine takes natural vegetal beel pterocarya stenoptera leaves and the like as the main raw materials, which are wide in resource, cheap and easy to obtain; through a simple process, the natural vegetable bee medicine which is remarkable in effect of driving mites and has no toxic side effect is produced, and can be used to kill large and small varroas in bee honeycombs; and bee colony tolerance is good, no chemical pesticides residue exists after use, and the problem that the medicine resources of demos is contested is avoided.
Description
Technical field
The invention belongs to the apiculture technical field, relate to the medicine of control honeybee mite, relate to particularly a kind of natural plants honeybee medicine and preparation method thereof.
Background technology
Acarine disease (acariasis ofbees) is the disease of the honeybee that caused by varoa mite and Mites, and very large to bee colony harm, the honeybee that suffers from the honeybee mite mostly is depauperation, have bad physiques, most larvas are just dead before not pupating, and sometimes even cause that full group is dead, brought heavy losses to apiculture.
The medicine that in the past is used for control honeybee mite mainly contained three classes: 1, fumigant: commonly used chlorfenethol is arranged, mite spirit and propargite etc. go out.Chlorfenethol pair has preferably smoked killing effect with the large and small honeybee mite that colonizes in into the honeybee body surface and creep on honeycomb, and shortcoming is easily to hinder young honeybee, so should not use when young honeybee measures the room greatly.Young honeybee is not hindered in the mite spirit of going out, but when early spring and late fall medication, tends to occur enclosing Wang Xianxiang.The propargite miticidal effect is good, and spring, summer, autumn all can use, and shortcoming is that excitant is large.2, fumigant: commonly used have camphor ball, sublimed sulfur and a sulphur naphthalene mixture etc.Camphor ball control varoa mite effect is better, and shortcoming is easily to hinder young honeybee.Sublimed sulfur also claims flowers of sulfur, is tiny yellow powder, for Mites lethality is arranged very, reaches uniform temperature and namely distils, and need keep properly.3, contact insecticide: mite must kill, mite pounces on etc. in commonly used having, and belongs to the plan chrysanthemum lipid drug of chemosynthesis, makes aqua or emulsion and is diluted to behind the finite concentration to cause harm the honeybee mite again and is sprayed directly on honeycomb and the bee colony the most prosperous season.The medicine of the above-mentioned control honeybee mite of mentioning all exists serious agricultural chemicals, chemical drug residual, and contaminated environment and bee product are the immediate causes that China's honey, propolis, pollen, emperor are starched the residual severe overweight of agricultural chemicals chemical drug.
Plant insecticide is to utilize some bioactivator that contains in some plant or the plant defense of inducing generation element, kills or the growing of some insect of establishment.The compound of killing insect in the plant corpus is that plant corpus produces multiplely has a secondary metabolite pest-resistant, antibacterial activity, alkaloids, flavonoid class, protein-based, organic acid and many different types such as phenolic compound have been comprised, such as capillin (Capillin), Chinese honey locust glycoside (Saponin), alkaloids etc.Once the someone reported that 1389 Plants might be as agricultural chemicals for the preparation of plant expelling parasite, bactericide.Based on above-mentioned situation, the research and development of recent domestic botanical acaricide launch one after another.For example, Korea Spro build brave wait the people adopt slide infusion process and blade infusion process tested respectively 10 Plants ternary mixture extracts (methyl alcohol: acetone: chloroform=23: 30: 47) and ligroin extraction to the acaricidal activity of citrus red mite (crm), found that Fengshun trifoliate jewelvine, Chinese Stellera Root, Plumbago zeylanica, sensitive plant and broomwort to kill the mite ovicidal action more remarkable, the miticidal effect of 0.01g/mL Fengshun trifoliate jewelvine, broomwort, sensitive plant ternary mixture extract and 1000 times of liquid of nimbin emulsion are without significant difference; The acaricidal rate that Fengshun trifoliate jewelvine ternary mixture extract and Chinese Stellera Root ligroin extraction 0.01g/mL24h tag reaches 100%; The ovicidal rate of Plumbago zeylanica, Chinese Stellera Root and japanese bugle herb ligroin extraction all reaches more than 90%, and particularly Plumbago zeylanica ternary extract, ligroin extraction and Chinese Stellera Root ligroin extraction ovicidal rate reach 100% or near 100%.The people such as Yang Huizhi summarize in the research aspect caricidal plant, acaricidal action mode and the mechanism of action the recent domestic botanical acaricide.Zhu Shuzhong points out that present caricidal plant mainly contains Meliaceae, composite family, Thymelaeceae, Solanaceae, pulse family, Chenopodiaceae, Labiatae, Carrot family etc. and amounts to about more than 100 kinds, and has looked forward to the plant resource miticide in the developing application prospect of the future of agriculture.But above-mentioned research focuses mostly on aspect caricidal plant resource, botanical garden miticide acaricidal action mode and the mechanism of action, and is far apart with widespread adoption.
CN101278681A discloses a kind of prescription of preventing and treating the honeybee mite, and following raw material is mixed with by volume: sweet wormwood oil 0.1-1.0ml, eucalyptus oil 0.1-1.0ml, lemon oil 0.1-1.0ml, litsea cubeba oil 0.1-1.0ml.This invention prescription is selected from numerous plants essential oils, controls mite effect and Ziyang mite and pounces on and compare difference and showing.
CN1830262A discloses a kind of medicine for the treatment of the large and small honeybee mite of honeybee, it is characterized in that medicament effective component is dissolved in the gel, thereby reduces in use airborne rate of volatilization of effective ingredient, prolongs the volatilization time, to reach the effect of spacetabs type medicament.This slow-release medicine, it mainly contains effective constituent is formic acid, eucalyptus oil, thymol, sodium carboxymethylcellulose or xanthans, water.
The above-mentioned disclosed caricidal plant resource overwhelming majority uses medicinal herb components, and what have is also more rare valuable, and existence and the people strive medicine source and problem with high costs.
Summary of the invention
One of the object of the invention is for the deficiencies in the prior art, a kind of natural plants honeybee medicine is provided, and it does not contain chemical synthetic drug and antibiotic take resource natural plants extensive and cheap and easy to get as primary raw material, noresidue, nuisanceless can be used for killing the large Mites in the honey comb.Two of the object of the invention is to provide the preparation method of described natural plants honeybee medicine.
In a first aspect of the present invention, adopt following technical scheme:
A kind of natural plants honeybee medicine comprises the raw material components of following percentage by weight: Chinese Wingmut Leaf medicinal extract 60wt%-90wt%, Tea Saponin 5wt%-20wt%, Radix Stemonae extract 2.8wt%-10wt%, Artemisia annua extract 2.1wt%-9wt%, Tween-80 0.1wt%-1wt%.
Chinese Wingmut Leaf medicinal extract of the present invention has significant killing parasites to relieve itshing effect, and it is not record in the prior art, is inventor's self-control, and the preparation method is as follows:
(1) gather Chinese Wingmut Leaf, clean up rear chopping, the blade diameter length ratio of packing into is the diacolation bucket of 1:5-20.
Preferably, described Chinese Wingmut Leaf be 8, the Chinese Wingmut Leaf of growth in September.
Preferably, described cleaning is that water cleans.
Described blade diameter length ratio is 1:5-20, for example can be 1:5-12.6,1:8.4-19.1,1:15-20,1:5,1:6,1:6.5,1:7,1:8,1:9,1:10,1:10.8,1:11,1:11.3,1:12,1:13,1:13.2,1:14,1:15,1:16,1:16.4,1:17,1:18,1:18.5,1:19,1:20; Be preferably 1:6-15; 1:10 more preferably.
(2) concentration that adds 2-10 times of quality of Chinese Wingmut Leaf is that the industrial alcohol of 60wt%-99wt% soaked 1-6 month.
Described 2-10 for example can be 2 times, 2.5 times, 3 times, 3.1 times, 3.8 times, 4 times, 4.5 times, 5 times, 5.6 times, 5.9 times, 6 times, 6.3 times, 6.5 times, 7 times, 7.3 times, 7.7 times, 8 times, 8.2 times, 8.5 times, 9 times, 9.1 times, 9.8 times, 10 times doubly; Be preferably 4-8 doubly; More preferably 6 times.
Described 60wt%-99wt% for example can be 60wt%-83.4wt%, 76.5wt%-91.7wt%, 63.2wt%-99wt%, 60wt%, 65wt%, 68.2wt%, 70wt%, 71.8wt%, 75wt%, 77.6wt%, 80wt%, 83.8wt%, 85wt%, 89.3wt%, 90wt%, 91wt%, 94.7wt%, 95wt%, 97.1wt%, 99wt%; Be preferably 85wt%-96wt%; 95wt% more preferably.
Described 1-6 month for example can be 1-3 month, individual month of 2.4-5.5,4.1-6 individual month, 1 month, 1.5 months, 1.8 months, 2 months, 2.5 months, 3 months, 3.3 months, 3.7 months, 4 months, 4.5 months, 5 months, 5.4 months, 5.9 months, 6 months; Be preferably 2-4 month; More preferably 3 months.
(3) collect percolate with the flow velocity of 0.5-2L/min.
The flow velocity of described 0.5-2L/min for example can be 0.5-1.3L/min, 0.7-1.6L/min, 1.4-2L/min, 0.5L/min, 0.6L/min, 0.7L/min, 0.8L/min, 0.85L/min, 0.9L/min, 0.93L/min, 1L/min, 1.1L/min, 1.2L/min, 1.28L/min, 1.3L/min, 1.4L/min, 1.5L/min, 1.52L/min, 1.6L/min, 1.7L/min, 1.8L/min, 1.9L/min, 2L/min; Be preferably 0.6-1.5L/min; 1L/min more preferably.
(4) with the percolate reduced pressure concentration of step (3), the volume after concentrating and the mass ratio of Chinese Wingmut Leaf inventory are 1:1, get Chinese Wingmut Leaf medicinal extract, simultaneously Recycled ethanol.
The percentage by weight of described Chinese Wingmut Leaf medicinal extract in natural plants honeybee medicine of the present invention is 60wt%-90wt%, for example can be 60wt%-83.7wt%, 75wt%-94.5wt%, 84.1wt%-90wt%, 60wt%, 62.3wt%, 65wt%, 66.8wt%, 70wt%, 71.9wt%, 75wt%, 78.2wt%, 80wt%, 82.3wt%, 84wt%, 85wt%, 87.4wt%, 90wt%; Be preferably 68wt%-86wt%; Most preferably be 80wt%.
Tea Saponin of the present invention is TS, is by a class candy glucoside compound that extracts in the tea tree seed, can select known Tea Saponin, such as the commercially available prod, perhaps extracts voluntarily according to the record of prior art, preferably buys the commercially available prod.Tea Saponin is as a kind of natural nonionic surface active agent, has good emulsification, dispersion, foaming, the function such as moistening, and has anti-inflammatory, analgesia, the pharmacological actions such as impermeabilisation.Can obviously improve the physicochemical property of agricultural chemicals after it joins in the agricultural chemicals, improve medicament effective accumulated amount on target, be beneficial to giving full play to of pesticide efficacy.Tea Saponin can also be degraded automatically, and nonhazardous can not exert an influence to the chemical property of agricultural chemicals in decomposable process, is conducive to the storage of agricultural chemicals.
The percentage by weight of described Tea Saponin in natural plants honeybee medicine of the present invention is 5wt%-20wt%, for example can be 5wt%-13.7wt%, 6.9wt%-18.5wt%, 9.1wt%-20wt%, 5wt%, 6wt%, 7.3wt%, 8wt%, 8.6wt%, 9wt%, 10wt%, 11.9wt%, 12.3wt%, 13wt%, 14.4wt%, 15wt%, 16wt%, 17.2wt%, 18wt%, 19.6wt%, 20wt%; Be preferably 7.5wt%-15wt%; Most preferably be 10wt%.
Radix Stemonae extract of the present invention is the active ingredients such as stemonine that extract from the dry rhizome of Stemonaceae plant radix stemonae sessilifoliae, Radix stemonae japonicae etc., has significant killing parasites to relieve itshing effect, can select known Radix Stemonae extract, such as the commercially available prod, perhaps extract voluntarily according to the record of prior art, preferably buy the commercially available prod.
The percentage by weight of described Radix Stemonae extract in natural plants honeybee medicine of the present invention is 2.8wt%-10wt%, for example can be 2.8wt%-7.7wt%, 4.9wt%-8.5wt%, 6.1wt%-10wt%, 2.8wt%, 2.9wt%, 3wt%, 3.4wt%, 3.5wt%, 4wt%, 4.6wt%, 5wt%, 5.2wt%, 6wt%, 6.5wt%, 7wt%, 7.3wt%, 8wt%, 8.6wt%, 9wt%, 10wt%; Be preferably 3.2wt%-8.2wt%; Most preferably be 5wt%.
Artemisia annua extract of the present invention is the active ingredients such as qinghaosu that the ground drying nest from the feverfew artemisia annua extracts, have significantly antibiotic, anti parasitic, the effect such as antipruritic, can select known Artemisia annua extract, such as the commercially available prod, perhaps extract voluntarily according to the record of prior art, preferably buy the commercially available prod.
The percentage by weight of described Artemisia annua extract in natural plants honeybee medicine of the present invention is 2.1wt%-9wt%, for example can be 2.1wt%-8.7wt%, 5.9wt%-7.5wt%, 4.4wt%-9wt%, 2.1wt%, 2.3wt%, 2.5wt%, 3wt%, 3.4wt%, 3.8wt%, 4wt%, 4.6wt%, 5wt%, 5.2wt%, 6wt%, 6.5wt%, 7wt%, 7.3wt%, 8wt%, 8.6wt%, 9wt%; Be preferably 3wt%-8wt%; Most preferably be 4.5wt%.
Described Tween-80 in natural plants honeybee medicine of the present invention as penetrating agent, help the performance of drug effect, its percentage by weight in natural plants honeybee medicine of the present invention is 0.1wt%-1wt%, for example can be 0.1wt%-0.7wt%, 0.25wt%-0.65wt%, 0.4wt%-1wt%, 0.1wt%, 0.15wt%, 0.2wt%, 0.3wt%, 0.37wt%, 0.4wt%, 0.5wt%, 0.56wt%, 0.6wt%, 0.7wt%, 0.8wt%, 0.82wt%, 0.9wt%, 1wt%; Be preferably 0.3wt%-0.8wt%; Most preferably be 0.5wt%.
Preferably, described natural plants honeybee medicine of the present invention is as follows:
A kind of natural plants honeybee medicine comprises the raw material components of following percentage by weight: Chinese Wingmut Leaf medicinal extract 65wt%-85wt%, Tea Saponin 8wt%-15wt%, Radix Stemonae extract 3.5wt%-8.5wt%, Artemisia annua extract 3wt%-8wt%, Tween-80 0.2wt%-0.8wt%.
Further preferably, described natural plants honeybee medicine of the present invention is as follows:
A kind of natural plants honeybee medicine comprises the raw material components of following percentage by weight: Chinese Wingmut Leaf medicinal extract 80wt%, Tea Saponin 10wt%, Radix Stemonae extract 5wt%, Artemisia annua extract 4.5wt%, Tween-80 0.5wt%.
" comprising " of the present invention, mean it except described component, can also contain other components, these other components are given described natural plants honeybee medicine with different characteristics.In addition, " comprising " of the present invention, can also replace with enclosed " being " or " by ... make ".No matter which kind of composition natural plants honeybee cartridge bag of the present invention draws together, the percentage by weight sum of described natural plants honeybee medicine is 100%.
In a second aspect of the present invention, adopt following technical scheme:
A kind of preparation method of natural plants honeybee medicine comprises the steps:
(1) first the 1/5-4/5 of whole Chinese Wingmut Leaf medicinal extract weight is joined in the mixer, again Tea Saponin, Radix Stemonae extract, Artemisia annua extract and Tween-80 are joined in the mixer.
Described 1/5-4/5 for example can be 1/5,1/4,1/3,2/5,1/2,3/5,2/3,3/4,4/5; Be preferably 2/5-3/5; Most preferably be 1/2.
Preferably, described mixer is stainless steel burden drum.
(2) middling speed stirs 20-60min.
Described middling speed stirs and refers to that rotating speed is 500-1000rpm, for example can be 500-841rpm, 625-937rpm, 567-1000rpm, 500rpm, 527rpm, 550rpm, 581rpm, 600rpm, 645rpm, 662rpm, 700rpm, 730rpm, 750rpm, 774rpm, 800rpm, 806rpm, 850rpm, 893rpm, 900rpm, 914rpm, 950rpm, 985rpm, 1000rpm; Be preferably 600-900rpm; Most preferably be 750rpm.
Described 20-60min for example can be 20-48.5min, 33-55min, 34.1-60min, 20min, 23.4min, 25min, 29.6min, 30min, 35min, 37.1min, 40min, 44.7min, 45min, 46.2min, 50min, 51.9min, 55min, 58.4min, 60min; Be preferably 25-40min; Most preferably be 30min.
(3) remaining Chinese Wingmut Leaf medicinal extract is joined in the mixer;
(4) middling speed stirs 5-20min, namely gets natural plants honeybee medicine of the present invention.
Described middling speed stirs and refers to that rotating speed is 500-1000rpm, for example can be 500-841rpm, 625-937rpm, 567-1000rpm, 500rpm, 527rpm, 550rpm, 581rpm, 600rpm, 645rpm, 662rpm, 700rpm, 730rpm, 750rpm, 774rpm, 800rpm, 806rpm, 850rpm, 893rpm, 900rpm, 914rpm, 950rpm, 985rpm, 1000rpm; Be preferably 600-900rpm; Most preferably be 750rpm.
Described 5-20min for example can be 5-11.5min, 7.3-15min, 14.1-20min, 5min, 5.4min, 6min, 6.6min, 7min, 7.1min, 8min, 8.7min, 9min, 9.2min, 10min, 10.9min, 11min, 12.4min, 13.5min, 14min, 15min, 16.7min, 17min, 17.5min, 18min, 19min, 19.3min, 20min; Be preferably 10-20min; Most preferably be 15min.
To sum up, the preparation method of a kind of natural plants honeybee medicine of the present invention comprises the steps: after the technical scheme optimization
(1) first the 2/5-3/5 of whole Chinese Wingmut Leaf medicinal extract weight is joined in the mixer, again Tea Saponin, Radix Stemonae extract, Artemisia annua extract and Tween-80 are joined in the mixer;
(2) middling speed stirs 25-40min under the rotating speed of 500-1000rpm;
(3) remaining Chinese Wingmut Leaf medicinal extract is joined in the mixer;
(4) middling speed stirs 10-20min under the rotating speed of 500-1000rpm, and get final product.
The preparation method of a kind of natural plants honeybee medicine of the present invention, technical scheme comprise the steps: after further optimizing
(1) first 1/2 of whole Chinese Wingmut Leaf medicinal extract weight is joined in the stainless steel burden drum, again Tea Saponin, Radix Stemonae extract, Artemisia annua extract and Tween-80 are joined in the mixer;
(2) middling speed stirs 30min under the rotating speed of 750rpm;
(3) remaining Chinese Wingmut Leaf medicinal extract is joined in the mixer;
(4) middling speed stirs 15min under the rotating speed of 750rpm, and get final product.
The application method of natural plants honeybee medicine provided by the invention, comprise the steps: to select fine weather, to shake up after mass volume ratio (mg/ml) dilution of natural plants honeybee medicine of the present invention according to 1:100-1:500, mentioning honeycomb after honeybee returns nest makes it to be 45° angle with level, spray to honeycomb, frame beam and honeybee road with the natural plants honeybee medicine after the dilution, make the honeybee body surface be covered with fumulus, every 3-5 days spray medicines once, the spray medicine is for 3-4 time a course for the treatment of.
Described 1:100-1:500 for example can be 1:100-1:369,1:127-1:434,1:350-1:500,1:100,1:109,1:150,1:185,1:200,1:234,1:250,1:260,1:300,1:347,1:390,1:400,1:411,1:450,1:478,1:500; Be preferably 1:150-1:300; Most preferably be 1:200.
The present invention is take resource natural plants Chinese Wingmut Leaf extensive and cheap and easy to get as primary raw material, be equipped with a small amount of Tea Saponin, Radix Stemonae extract and Artemisia annua extract, by succinct technique, make and drive the natural plants honeybee medicine that the mite effect is remarkable, have no side effect, can be used for killing the large Mites in the honey comb.Because the material composition of natural plants honeybee medicine of the present invention all is natural plant composition cheap and easy to get basically, does not therefore exist with the people and strive medicine source and problem with high costs; Simultaneously owing to wherein do not contain chemical synthetic drug and antibiotic, therefore bee colony well-tolerated has not only also solved the residue problem of chemical synthetic pesticide, antibiotic veterinary drug in the bee product.Natural plants honeybee medicine of the present invention is a kind of noresidue, plant resource honeybee nuisanceless, high-drug-effect the mite preparation of dispelling.
Description of drawings
Fig. 1 is that beehive nest doorway has the large Mites photo of being hauled out by honeybee after using natural plants honeybee medicine of the present invention in the test example 1.
Fig. 2 is the normal activity photo of using bee colony behind the natural plants honeybee medicine of the present invention in the test example 2.
Further specify technical scheme of the present invention below in conjunction with accompanying drawing and by embodiment; but following example only is simple and easy example of the present invention; do not represent or limit the scope of the present invention, protection scope of the present invention is as the criterion with claims.
Embodiment
For the present invention is described better, be convenient to understand technical scheme of the present invention, typical but non-limiting embodiment of the present invention is as follows:
Embodiment 1:
Be prepared as follows natural plants honeybee medicine:
(1) gather the Chinese Wingmut Leaf 5kg that grows at the beginning of 8 months, clean up rear chopping with running water, placing blade diameter length ratio is the Chinese medicine diacolation bucket of 1:10;
(2) adding 30kg concentration is that 95% industrial alcohol soaked 3 months;
(3) collect percolate with the flow velocity of 1L/min;
(4) percolate with step (3) is evaporated to 5L, gets Chinese Wingmut Leaf medicinal extract, simultaneously Recycled ethanol;
(5) the Chinese Wingmut Leaf medicinal extract that first 2.5L step (4) is obtained joins in the stainless steel burden drum, 0.625kg Tea Saponin (Zhengzhou litchi promise bio tech ltd), 0.3125kg Radix Stemonae extract (the precious outstanding plant in Ankang city Co., Ltd), 0.28125kg Artemisia annua extract (Xi'an Sai Bang Pharmaceutical Technology Co., Ltd) and 0.03125kg Tween-80 is joined in the mixer again;
(6) stir 30min under the rotating speed of 750rpm;
(7) remaining 2.5L Chinese Wingmut Leaf medicinal extract is joined in the stainless steel burden drum;
(8) stir 15min under the rotating speed of 750rpm, namely get 6.25kg natural plants honeybee medicine.
Embodiment 2:
Be prepared as follows natural plants honeybee medicine:
(1) gather the Chinese Wingmut Leaf 10kg that grows at the beginning of 9 months, clean up rear chopping with running water, placing blade diameter length ratio is the Chinese medicine diacolation bucket of 1:5;
(2) adding 100kg concentration is that the industrial alcohol of 60wt% soaked 1 month;
(3) collect percolate with the flow velocity of 0.5L/min;
(4) percolate with step (3) is evaporated to 10L, gets Chinese Wingmut Leaf medicinal extract, simultaneously Recycled ethanol;
(5) the Chinese Wingmut Leaf medicinal extract that first 2L step (4) is obtained joins in the stainless steel burden drum, 3.33kg Tea Saponin (Zhengzhou litchi promise bio tech ltd), 1.67kg Radix Stemonae extract (the precious outstanding plant in Ankang city Co., Ltd), 1.5kg Artemisia annua extract (Xi'an Sai Bang Pharmaceutical Technology Co., Ltd) and 0.17kg Tween-80 is joined in the mixer again;
(6) stir 60min under the rotating speed of 500rpm;
(7) remaining 8L Chinese Wingmut Leaf medicinal extract is joined in the stainless steel burden drum;
(8) stir 20min under the rotating speed of 500rpm, namely get 16.67kg natural plants honeybee medicine.
Embodiment 3:
Be prepared as follows natural plants honeybee medicine:
(1) gather the by the end of September Chinese Wingmut Leaf 20kg of growth, clean up rear chopping with running water, placing blade diameter length ratio is the Chinese medicine diacolation bucket of 1:20;
(2) adding 40kg concentration is that the industrial alcohol of 99wt% soaked 6 months;
(3) collect percolate with the flow velocity of 2L/min;
(4) percolate with step (3) is evaporated to 20L, gets Chinese Wingmut Leaf medicinal extract, simultaneously Recycled ethanol;
(5) the Chinese Wingmut Leaf medicinal extract that first 16L step (4) is obtained joins in the stainless steel burden drum, 1.11kg Tea Saponin (Zhengzhou litchi promise bio tech ltd), 0.62kg Radix Stemonae extract (the precious outstanding plant in Ankang city Co., Ltd), 0.47kg Artemisia annua extract (Xi'an Sai Bang Pharmaceutical Technology Co., Ltd) and 0.02kg Tween-80 is joined in the mixer again;
(6) stir 20min under the rotating speed of 1000rpm;
(7) remaining 4L Chinese Wingmut Leaf medicinal extract is joined in the stainless steel burden drum;
(8) stir 5min under the rotating speed of 1000rpm, namely get 22.22kg natural plants honeybee medicine.
Embodiment 4:
Be prepared as follows natural plants honeybee medicine:
(1) gather the by the end of August Chinese Wingmut Leaf 10kg of growth, clean up rear chopping with running water, placing blade diameter length ratio is the Chinese medicine diacolation bucket of 1:15;
(2) adding 40kg concentration is that the industrial alcohol of 80wt% soaked 4 months;
(3) collect percolate with the flow velocity of 1.5L/min
(4) percolate with step (3) is evaporated to 10L, gets Chinese Wingmut Leaf medicinal extract, simultaneously Recycled ethanol;
(5) the Chinese Wingmut Leaf medicinal extract that first 4L step (4) is obtained joins in the stainless steel burden drum, 2.21kg Tea Saponin (Zhengzhou litchi promise bio tech ltd), 1.21kg Radix Stemonae extract (the precious outstanding plant in Ankang city Co., Ltd), 1.18kg Artemisia annua extract (Xi'an Sai Bang Pharmaceutical Technology Co., Ltd) and 0.12kg Tween-80 is joined in the mixer again;
(6) stir 40min under the rotating speed of 600rpm;
(7) remaining 6L Chinese Wingmut Leaf medicinal extract is joined in the stainless steel burden drum;
(8) stir 15min under the rotating speed of 600rpm, namely get 14.72kg natural plants honeybee medicine.
Embodiment 5:
Be prepared as follows natural plants honeybee medicine:
(1) gather the Chinese Wingmut Leaf 5kg that grows at the beginning of 8 months, clean up rear chopping with running water, placing blade diameter length ratio is the Chinese medicine diacolation bucket of 1:6;
(2) adding 40kg concentration is that the industrial alcohol of 96wt% soaked 2 months;
(3) collect percolate with the flow velocity of 0.6L/min;
(4) percolate with step (3) is evaporated to 5L, gets Chinese Wingmut Leaf medicinal extract, simultaneously Recycled ethanol;
(5) the Chinese Wingmut Leaf medicinal extract that first 3L step (4) is obtained joins in the stainless steel burden drum, 0.436kg Tea Saponin (Zhengzhou litchi promise bio tech ltd), 0.186kg Radix Stemonae extract (the precious outstanding plant in Ankang city Co., Ltd), 0.174kg Artemisia annua extract (Xi'an Sai Bang Pharmaceutical Technology Co., Ltd) and 0.017kg Tween-80 is joined in the mixer again;
(6) stir 25min under the rotating speed of 900rpm;
(7) remaining 2L Chinese Wingmut Leaf medicinal extract is joined in the stainless steel burden drum;
(8) stir 10min under the rotating speed of 900rpm, namely get 5.813kg natural plants honeybee medicine.
Test example 1:
Test the mite effect of dispelling of natural plants honeybee medicine of the present invention, method is as follows:
Shake up after the volume ratio dilution of natural plants honeybee medicine according to 1:200 with embodiment 1 acquisition, get the natural plants honeybee medicine of bio-occlusion concentration.
In the bee-keeping base, select fine weather, after honeybee returns nest, mention honeycomb and make it to be 45° angle with level, spray to honeycomb, frame beam and honeybee road with the natural plants honeybee medicine after the dilution, make the honeybee body surface be covered with fumulus, every 5 days spray medicines once, the spray medicine is for 3 times a course for the treatment of.
Result of the test as shown in Figure 1, only after spraying medicine 3h for the first time, beehive nest doorway just has the large Mites of being hauled out by honeybee, shows to dispel natural plants honeybee medicine of the present invention to kill the effect of mite remarkable for mite.
Test example 2:
Test safety and the bee colony tolerance of natural plants honeybee medicine of the present invention, method is as follows:
Shake up after the volume ratio dilution of natural plants honeybee medicine according to 1:200 with embodiment 1 acquisition, get the natural plants honeybee medicine of bio-occlusion concentration; The natural plants honeybee medicine of bio-occlusion concentration is concentrated 10 times, for subsequent use as trial drug.
In the bee-keeping base, select fine weather, after honeybee returns nest, mention honeycomb and make it to be 45° angle with level, to honeycomb, frame beam and the spraying of honeybee road, make the honeybee body surface be covered with fumulus with trial drug, every 3 days spray medicines once, the spray medicine is for 4 times a course for the treatment of.
Result of the test as shown in Figure 2, bee colony normal activity still after finishing course for the treatment of, well-tolerated, without climbing the honeybee phenomenon, larvae development is good, shows that natural plants honeybee medicine bee colony of the present invention tolerates safety non-toxic.
Test example 3:
With chemosynthesis acaricide " taufluvalinate " commonly used at present as positive control, select two groups of identical bee colonies, every group of bee colony includes continue 2 of hive bee groups of 3 of the flat hive bee groups of large Mites and band, two groups of bee colonies are respectively at 5 of early mornings giving the natural plants honeybee medicine of taufluvalinate and embodiment 1 by bee-keeping person, the anti-mite effect of two kinds of medicines relatively behind the administration 3h.As a result shown in the following table:
| ? | The taufluvalinate group | Natural plants honeybee medicine group |
| Kill varoa mite | Effect is remarkable | Effect is remarkable |
| Kill Mites | Effect is remarkable | Effect is remarkable |
| Climb the honeybee rate | 15%-20% | 1%-2% |
| Chemical drug is residual | Have | Nothing |
Result of the test shows, natural plants honeybee medicine of the present invention is suitable with taufluvalinate aspect the effect of acarid extremely of dispelling, but bee colony tolerance, toxic and side effect and chemical drug residual aspect will, natural plants honeybee medicine of the present invention will obviously be better than taufluvalinate.
Should be noted that and understand, in the situation that does not break away from the desired the spirit and scope of the present invention of accompanying claim, can make to the present invention of foregoing detailed description various modifications and improvement.Therefore, the scope of claimed technical scheme is not subjected to the restriction of given any specific exemplary teachings.
Applicant's statement, the present invention illustrates raw material proportioning of the present invention and preparation process by above-described embodiment, but the present invention is not limited to said ratio and preparation process, does not mean that namely the present invention must rely on above-mentioned preparation process and could implement.The person of ordinary skill in the field should understand, any improvement in the present invention to the interpolation of the equivalence replacement of the selected raw material of the present invention and auxiliary element, the selection of concrete mode etc., all drops within protection scope of the present invention and the open scope.
Claims (10)
1. natural plants honeybee medicine, it is characterized in that described honeybee cartridge bag is drawn together the raw material components of following percentage by weight: Chinese Wingmut Leaf medicinal extract 60wt%-90wt%, Tea Saponin 5wt%-20wt%, Radix Stemonae extract 2.8wt%-10wt%, Artemisia annua extract 2.1wt%-9wt%, Tween-80 0.1wt%-1wt%.
2. natural plants honeybee medicine according to claim 1, it is characterized in that described honeybee cartridge bag is drawn together the raw material components of following percentage by weight: Chinese Wingmut Leaf medicinal extract 68wt%-86wt%, Tea Saponin 7.5wt%-15wt%, Radix Stemonae extract 3.2wt%-8.2wt%, Artemisia annua extract 3wt%-8wt%, Tween-80 0.3wt%-0.8wt%.
3. natural plants honeybee medicine according to claim 1 and 2, it is characterized in that described honeybee cartridge bag is drawn together the raw material components of following percentage by weight: Chinese Wingmut Leaf medicinal extract 80wt%, Tea Saponin 10wt%, Radix Stemonae extract 5wt%, Artemisia annua extract 4.5wt%, Tween-80 0.5wt%.
4. described natural plants honeybee medicine one of according to claim 1-3 is characterized in that the preparation method of described Chinese Wingmut Leaf medicinal extract is as follows:
(1) gather Chinese Wingmut Leaf, clean up rear chopping, the blade diameter length ratio of packing into is the diacolation bucket of 1:5-20;
(2) concentration that adds 2-10 times of quality of Chinese Wingmut Leaf is that the industrial alcohol of 60wt%-99wt% soaked 1-6 month;
(3) collect percolate with the flow velocity of 0.5-2L/min;
(4) with the percolate reduced pressure concentration of step (3), the volume after concentrating and the mass ratio (mg/ml) of Chinese Wingmut Leaf inventory are 1:1, get Chinese Wingmut Leaf medicinal extract, simultaneously Recycled ethanol.
5. natural plants honeybee medicine according to claim 4 is characterized in that, in the preparation method's of described Chinese Wingmut Leaf medicinal extract the step (1), described Chinese Wingmut Leaf is 8, the Chinese Wingmut Leaf of growth in September;
Preferably, described blade diameter length ratio is 1:6-15; 1:10 more preferably.
6. according to claim 4 or 5 described natural plants honeybee medicines, it is characterized in that, in the preparation method's of described Chinese Wingmut Leaf medicinal extract the step (2), the addition of described industrial alcohol be the Chinese Wingmut Leaf quality 4-8 doubly; More preferably 6 times;
Preferably, the concentration of described industrial alcohol is 85wt%-96wt%; 95wt% more preferably;
Preferably, described soak time is 2-4 month; More preferably 3 months.
7. one of according to claim 4-6 described natural plants honeybee medicine is characterized in that in the preparation method's of described Chinese Wingmut Leaf medicinal extract the step (3), described flow velocity is 0.6-1.5L/min; Be preferably 1L/min.
8. one of according to claim 1-7 the preparation method of described natural plants honeybee medicine is characterized in that described method comprises the steps:
(1) first the 1/5-4/5 of whole Chinese Wingmut Leaf medicinal extract weight is joined in the mixer, again Tea Saponin, Radix Stemonae extract, Artemisia annua extract and Tween-80 are joined in the mixer;
(2) middling speed stirs 20-60min;
(3) remaining Chinese Wingmut Leaf medicinal extract is joined in the mixer;
(4) middling speed stirs 5-20min, namely gets natural plants honeybee medicine of the present invention.
9. one of according to claim 1-8 described preparation method is characterized in that described method comprises the steps:
(1) first the 2/5-3/5 of whole Chinese Wingmut Leaf medicinal extract weight is joined in the mixer, again Tea Saponin, Radix Stemonae extract, Artemisia annua extract and Tween-80 are joined in the mixer;
(2) middling speed stirs 25-40min under the rotating speed of 500-1000rpm;
(3) remaining Chinese Wingmut Leaf medicinal extract is joined in the mixer;
(4) middling speed stirs 10-20min under the rotating speed of 500-1000rpm, and get final product.
10. one of according to claim 1-9 described preparation method is characterized in that described method comprises the steps:
(1) first 1/2 of whole Chinese Wingmut Leaf medicinal extract weight is joined in the stainless steel burden drum, again Tea Saponin, Radix Stemonae extract, Artemisia annua extract and Tween-80 are joined in the mixer;
(2) stir 30min under the rotating speed of 750rpm;
(3) remaining Chinese Wingmut Leaf medicinal extract is joined in the stainless steel burden drum;
(4) stir 15min under the rotating speed of 750rpm, and get final product.
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Address after: 102200 Beijing science and Technology Park of Changping District Bai Fu Road 13 Patentee after: BEIJING ZHI FENG TANG HEALTH TECHNOLOGY CO., LTD. Address before: 102200 Beijing science and Technology Park of Changping District Bai Fu Road 13 Patentee before: Beijing Zhifengtang Bee Product Co., Ltd. |