CN107307012B - Application of szechwan chinaberry fruit in preventing and treating bee mites - Google Patents
Application of szechwan chinaberry fruit in preventing and treating bee mites Download PDFInfo
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- CN107307012B CN107307012B CN201710582870.8A CN201710582870A CN107307012B CN 107307012 B CN107307012 B CN 107307012B CN 201710582870 A CN201710582870 A CN 201710582870A CN 107307012 B CN107307012 B CN 107307012B
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- 235000013399 edible fruits Nutrition 0.000 title claims abstract description 87
- 241000238876 Acari Species 0.000 title claims abstract description 35
- 240000000233 Melia azedarach Species 0.000 title abstract description 52
- 239000000843 powder Substances 0.000 claims abstract description 78
- 238000000227 grinding Methods 0.000 claims abstract description 28
- 238000002360 preparation method Methods 0.000 claims abstract description 4
- 238000007873 sieving Methods 0.000 claims description 13
- 238000004140 cleaning Methods 0.000 claims description 9
- 238000001035 drying Methods 0.000 claims description 9
- 238000011221 initial treatment Methods 0.000 claims description 9
- 230000002265 prevention Effects 0.000 claims description 9
- 238000011282 treatment Methods 0.000 claims description 9
- 210000000952 spleen Anatomy 0.000 claims description 5
- 230000007480 spreading Effects 0.000 claims description 2
- 150000001875 compounds Chemical class 0.000 abstract description 43
- NINIDFKCEFEMDL-UHFFFAOYSA-N Sulfur Chemical compound [S] NINIDFKCEFEMDL-UHFFFAOYSA-N 0.000 abstract description 22
- 241000257303 Hymenoptera Species 0.000 abstract description 16
- 230000000694 effects Effects 0.000 abstract description 11
- 235000013601 eggs Nutrition 0.000 abstract description 5
- 230000000895 acaricidal effect Effects 0.000 abstract description 4
- 241000196324 Embryophyta Species 0.000 abstract description 2
- 239000004480 active ingredient Substances 0.000 abstract 1
- 241000256844 Apis mellifera Species 0.000 description 44
- 238000012360 testing method Methods 0.000 description 13
- 238000002156 mixing Methods 0.000 description 12
- QXAITBQSYVNQDR-ZIOPAAQOSA-N amitraz Chemical compound C=1C=C(C)C=C(C)C=1/N=C/N(C)\C=N\C1=CC=C(C)C=C1C QXAITBQSYVNQDR-ZIOPAAQOSA-N 0.000 description 6
- 229960002587 amitraz Drugs 0.000 description 6
- 239000003814 drug Substances 0.000 description 6
- 239000011261 inert gas Substances 0.000 description 6
- 238000003756 stirring Methods 0.000 description 6
- 238000002474 experimental method Methods 0.000 description 5
- 230000003071 parasitic effect Effects 0.000 description 5
- 238000012216 screening Methods 0.000 description 5
- 241001465754 Metazoa Species 0.000 description 4
- 239000000463 material Substances 0.000 description 4
- 238000000034 method Methods 0.000 description 4
- 238000007789 sealing Methods 0.000 description 4
- 231100000331 toxic Toxicity 0.000 description 4
- 230000002588 toxic effect Effects 0.000 description 4
- 239000000642 acaricide Substances 0.000 description 3
- 201000010099 disease Diseases 0.000 description 3
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 3
- 230000036541 health Effects 0.000 description 3
- 238000005507 spraying Methods 0.000 description 3
- 239000004575 stone Substances 0.000 description 3
- 241000628997 Flos Species 0.000 description 2
- 241000158728 Meliaceae Species 0.000 description 2
- 241000607479 Yersinia pestis Species 0.000 description 2
- 244000144987 brood Species 0.000 description 2
- 229940079593 drug Drugs 0.000 description 2
- 210000004185 liver Anatomy 0.000 description 2
- 239000013642 negative control Substances 0.000 description 2
- 230000000361 pesticidal effect Effects 0.000 description 2
- FFRBMBIXVSCUFS-UHFFFAOYSA-N 2,4-dinitro-1-naphthol Chemical group C1=CC=C2C(O)=C([N+]([O-])=O)C=C([N+]([O-])=O)C2=C1 FFRBMBIXVSCUFS-UHFFFAOYSA-N 0.000 description 1
- 208000004998 Abdominal Pain Diseases 0.000 description 1
- 241000256837 Apidae Species 0.000 description 1
- 241000256836 Apis Species 0.000 description 1
- 241000256846 Apis cerana Species 0.000 description 1
- 241000256848 Apis florea Species 0.000 description 1
- 241001076188 Apis laboriosa Species 0.000 description 1
- 208000003508 Botulism Diseases 0.000 description 1
- 241000759833 Cornus officinalis Species 0.000 description 1
- 206010049077 Hernia pain Diseases 0.000 description 1
- 241000238631 Hexapoda Species 0.000 description 1
- 241000264877 Hippospongia communis Species 0.000 description 1
- 206010061217 Infestation Diseases 0.000 description 1
- 241001126829 Nosema Species 0.000 description 1
- 208000002193 Pain Diseases 0.000 description 1
- 241001558516 Varroa destructor Species 0.000 description 1
- 230000003213 activating effect Effects 0.000 description 1
- 230000000507 anthelmentic effect Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 235000019658 bitter taste Nutrition 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 244000078703 ectoparasite Species 0.000 description 1
- 238000011156 evaluation Methods 0.000 description 1
- 239000010419 fine particle Substances 0.000 description 1
- 230000012447 hatching Effects 0.000 description 1
- 208000027866 inflammatory disease Diseases 0.000 description 1
- 230000002401 inhibitory effect Effects 0.000 description 1
- 239000010985 leather Substances 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 201000002266 mite infestation Diseases 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 210000001672 ovary Anatomy 0.000 description 1
- 244000045947 parasite Species 0.000 description 1
- 230000024241 parasitism Effects 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- 239000013641 positive control Substances 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- -1 re Species 0.000 description 1
- 230000005070 ripening Effects 0.000 description 1
- 210000000813 small intestine Anatomy 0.000 description 1
- 235000019614 sour taste Nutrition 0.000 description 1
- 238000009331 sowing Methods 0.000 description 1
- 238000010998 test method Methods 0.000 description 1
- 210000001519 tissue Anatomy 0.000 description 1
- 231100000419 toxicity Toxicity 0.000 description 1
- 230000001988 toxicity Effects 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
- 210000003932 urinary bladder Anatomy 0.000 description 1
- 230000017260 vegetative to reproductive phase transition of meristem Effects 0.000 description 1
- 210000003462 vein Anatomy 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N59/00—Biocides, pest repellants or attractants, or plant growth regulators containing elements or inorganic compounds
- A01N59/02—Sulfur; Selenium; Tellurium; Compounds thereof
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
- A01N65/26—Meliaceae [Chinaberry or Mahogany family], e.g. mahogany, langsat or neem
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- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Dentistry (AREA)
- Agronomy & Crop Science (AREA)
- Environmental Sciences (AREA)
- Wood Science & Technology (AREA)
- Plant Pathology (AREA)
- Mycology (AREA)
- Biotechnology (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Microbiology (AREA)
- Chemical & Material Sciences (AREA)
- Inorganic Chemistry (AREA)
- Pest Control & Pesticides (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention discloses an application of toosendan fruit in preventing and treating bee mites, and provides toosendan fruit powder, toosendan fruit compound powder and a preparation method thereof. The toosendan fruit serving as an acaricidal component is a plant tissue, is more environment-friendly and pollution-free to bee products, and is safe to adult bees, young bees, eggs and queen bees; the superfine grinding is adopted to ensure that the granularity of the szechwan chinaberry fruit powder and the szechwan chinaberry fruit compound powder is finer, the utilization rate of active ingredients is higher, and the effect of preventing and treating bee mites is better than that of sublimed sulfur.
Description
Technical Field
The invention relates to the field of bee disease control, in particular to application of szechwan chinaberry fruit in controlling bee mites.
Background
Bee mites (tropilaeps spp.) are important pest mites of the honeybee family (Apidae) in asian areas. It has fast reproduction and short sowing period, and is a bee ectoparasite with greater harm than Varroa destructor. The Apis cerana primary host is the large bee (Apis dorsa), but can also transfer hosts, infecting the western bees (a. mellifera), the black large bee (a. laboriosa) and the small bee (a. florea) of the family meliae.
At present, the most common medicaments for preventing and treating bee mites at home are of two types: one is a powder product, mainly comprising sublimed sulfur powder and compound sublimed sulfur powder; one is aqueous product, mainly amitraz.
The sublimed sulfur powder and the compound sublimed sulfur powder are adopted to prevent and control the small bee mites, the medicinal powder needs to be uniformly scattered on bee roads and frame beams, and can also be directly smeared on the sealing cover spleen, but the problems are as follows:
1) the powder has coarse granularity and low utilization rate of the medicine;
2) the control effect is reduced after long-term use, and the dosage of the powder needs to be increased;
3) has certain toxic action on bees, and is mainly manifested by no hatching of eggs, no laying of eggs by queens, and the like.
The amitraz serving as an aqueous product is adopted to prevent and treat the small bee mites, all honeycombs in a beehive need to be lifted one by one for spraying to treat the mites, but the problems are as follows:
1) the spraying operation is carried out one by one, which takes time and labor;
2) the prevention and treatment effect is poor compared with that of powder products;
3) amitraz is more toxic to humans, and residue can affect the quality of bee products. The european union has been banned on bees.
Toosendan fruit, also known as melia toosendan fruit, fructus toosendan, zizyphi fructus, melia azedarach, melia toosendan, cornus officinalis, latin character: meliatoosendanan sieb. Fruit of Melia toosendan (Chinaberry) belonging to Meliaceae, deciduous tree, with 10 m higher than original seed; the young branches are densely covered with brown star-shaped scales, are dark red, have skin holes and have obvious leaf marks when being old. 2, the length of the pinnate complex leaf is 35-45 cm, and each 1 pinnate has 4-5 pairs of small leaves; a long handle is provided; the cotyledon is opposite, has short handle or nearly no handle, membranous, elliptic shape with needle shape, length of 4-10 cm, width of 2-4.5 cm, tip of taper, wedge shape or nearly round shape of base, no hair on both sides, full margin or with unobvious blunt teeth, 12-14 pairs of side veins, nearly spherical ovary, no hair, 6-8 chambers, nearly cylindrical style of style, no hair, 6-dentate cleft of stigma which is not obvious, and is occluded in the stamen tube. Big stone fruit, elliptic spherical shape, length of about 3 cm, width of about 2.5 cm, thin peel, and light yellow after ripening; the nucleus is slightly rigid, 6-8 chambers. The flowering period is 3-4 months, the fruit period is 10-11 months, the fruits are similar to spheres, and the diameters of the fruits are 2-3 cm. The surface is golden yellow to brown yellow, slightly lustrous and has dark brown dots. The top end is provided with style residue, and the base part is sunken with fruit stalk marks. The exocarp leather is often in a gap with the pulp, and the pulp is soft and light yellow and shows stickiness after being wetted by water. The fruit stone is spherical or oval, hard in texture, truncated at two ends, and provided with 6-8 longitudinal edges, the interior of the fruit stone is divided into 6-8 chambers, and each chamber contains 1 black-brown oblong seed. Special smell, sour and bitter taste.
Chuan Lian Zi is a commonly used clinical herb. Chinaberry fruit carried in 2005 edition from the Chinese pharmacopoeia is a dry mature fruit of Melia toosendan of Meliaceae, and is bitter and cold in nature and slightly toxic. It belongs to liver, small intestine and bladder channels, and has effects of dispersing stagnated liver qi, clearing heat, activating qi-flowing, relieving pain, and killing parasite. It is mainly used for chest and hypochondrium, hernia pain, abdominal pain due to parasitic infestation, etc. in clinic, and is also used for various inflammatory diseases when being combined with other medicines.
Fructus Toosendan has anthelmintic effect, and also has effects of inhibiting central system and resisting animal botulism. The toosendan fruit is used for preventing and treating bee diseases and insect pests, only the patent of application number 201510302600.8 is reported at present, and the toosendan fruit is mainly used for preventing and treating the nosema disease of bees. The application of the szechwan chinaberry fruit to the control of bee mites has not been reported.
Disclosure of Invention
The invention aims to: compared with the prior art, the method can reduce the toxic effect of the medicament on bees, improve the control effect of the wasp mites and does not influence the quality of bee products.
The invention is realized by the following technical scheme:
the invention provides application of szechwan chinaberry fruit in prevention and treatment of bee mites, and mainly provides szechwan chinaberry fruit powder for prevention and treatment of bee mites.
The preparation method of the szechwan chinaberry fruit powder comprises the following steps:
1) primary treatment: cleaning fructus Toosendan, and sun drying;
2) primary crushing: adding the primarily treated fructus toosendan into a grinder for primary grinding;
3) sieving: sieving the primarily pulverized fructus Toosendan with 50-70 mesh sieve;
4) superfine grinding: the toosendan fruit powder with the powder granularity D90 less than or equal to 23 microns is obtained by superfine grinding.
The fructus Toosendan is fruit of fructus Toosendan (including outer skin and inner core).
The invention also provides application of the szechwan chinaberry fruit compound powder in prevention and treatment of bee mites.
The toosendan fruit compound powder contains a compound material.
The compound material is one or more of sublimed sulfur, fructus gleditsiae, flos genkwa and flos genkwa essence.
One of the preferred modes of the compound material is sublimed sulfur. The toxicity of sublimed sulfur is effectively reduced by adding the szechwan chinaberry fruit.
The mass content of the szechwan chinaberry fruit in the szechwan chinaberry fruit compound powder is 1-99%, and the mass content of sublimed sulfur is 99-1%.
In one preferable mode of the toosendan fruit compound powder, the toosendan fruit mass content in the toosendan fruit compound powder is 90-99%, and the mass content of sublimed sulfur is 1-10%.
In one preferable mode of the toosendan fruit compound powder, the toosendan fruit mass content in the toosendan fruit compound powder is 99%, and the mass content of sublimed sulfur is 1%.
The preparation method of the szechwan chinaberry fruit compound powder comprises the following steps:
1) primary treatment: cleaning fructus Toosendan, and sun drying;
2) primary crushing: adding the primarily treated fructus toosendan into a grinder for primary grinding;
3) sieving: sieving the primarily pulverized fructus Toosendan with 50-70 mesh sieve;
4) mixing: mixing fructus Toosendan and the compound material, and stirring;
5) superfine grinding: under the protection of inert gas, the toosendan fruit compound powder with the powder granularity D90 being less than or equal to 23 microns is obtained through superfine grinding.
The fructus Toosendan is fruit of fructus Toosendan (including outer skin and inner core).
The invention provides application of the szechwan chinaberry fruit powder and the szechwan chinaberry fruit compound powder in prevention and treatment of bee mites, and the szechwan chinaberry fruit powder and the szechwan chinaberry fruit compound powder are put in a mode of spreading bee colonies and frame beams, and can also be directly smeared on a capped spleen.
Compared with the prior art, the invention has the following advantages and beneficial effects:
1) the effect of the szechwan chinaberry fruit in preventing and treating bee mites is excellent;
2) the powder has a powder particle size D90 of less than or equal to 23 microns, fine particle size and high utilization rate of effective components;
3) the fructus Toosendan is used for preventing and treating bee mites, and is safe for adult bees, brood bees, eggs and queen bees;
4) the mite-killing component, namely the szechwan chinaberry fruit, is plant tissue, is more environment-friendly to bee products, and has no pollution.
Detailed Description
All of the features disclosed in this specification, or all of the steps of any method or process so disclosed, may be combined in any combination, except features and/or steps which are mutually exclusive, unless expressly stated otherwise, with other alternative features which are equivalent or similar in purpose, i.e. each feature is an embodiment of a range of equivalent or similar features, unless expressly stated otherwise.
The reagents used in the invention are mainly as follows: the acaricide of the control group is sold as sublimed sulfur, and the main component is sublimed sulfur (the manufacturer is Wang animal health Limited liability company in Sichuan). The main component of the agent for checking the prevention and treatment effect is amitraz (the manufacturer is Wang animal health care Limited liability company in Sichuan).
The toosendan fruit used in the invention is a toosendan fruit, comprising a skin and a kernel.
Example 1:
preparing szechwan chinaberry fruit powder 1:
1) primary treatment: cleaning fructus Toosendan, and sun drying;
2) primary crushing: adding the primarily treated fructus toosendan into a grinder for primary grinding;
3) sieving: putting the primarily crushed szechwan chinaberry fruit into a 3-layer electronic vibrating screen (50 meshes) for screening;
4) superfine grinding: the toosendan fruit powder 1 with the powder granularity D90 less than or equal to 23 microns is obtained by superfine grinding.
Example 2:
preparing the szechwan chinaberry fruit compound powder 1:
1) primary treatment: cleaning fructus Toosendan, and sun drying;
2) primary crushing: adding the primarily treated fructus toosendan into a grinder for primary grinding;
3) sieving: putting the primarily crushed szechwan chinaberry fruit into a 3-layer electronic vibrating screen (60 meshes) for screening;
4) mixing: mixing fructus Toosendan (99 wt.%) with sublimed sulfur (1 wt.%), and stirring;
5) superfine grinding: under the protection of inert gas, the toosendan fruit compound powder 1 with the powder granularity D90 being less than or equal to 23 microns is obtained through superfine grinding.
Example 3:
preparing the szechwan chinaberry fruit compound powder 2:
1) primary treatment: cleaning fructus Toosendan, and sun drying;
2) primary crushing: adding the primarily treated fructus toosendan into a grinder for primary grinding;
3) sieving: putting the primarily crushed szechwan chinaberry fruit into a 3-layer electronic vibrating screen (70 meshes) for screening;
4) mixing: mixing fructus Toosendan (90 wt.%) with sublimed sulfur (10 wt.%), and stirring;
5) superfine grinding: under the protection of inert gas, the toosendan fruit compound powder 2 with the powder granularity D90 being less than or equal to 23 microns is obtained through superfine grinding.
Example 4:
preparing the szechwan chinaberry fruit compound powder 3:
1) primary treatment: cleaning fructus Toosendan, and sun drying;
2) primary crushing: adding the primarily treated fructus toosendan into a grinder for primary grinding;
3) sieving: putting the primarily crushed szechwan chinaberry fruit into an electronic vibrating screen (50 meshes) for screening;
4) mixing: mixing fructus Toosendan (50 wt.%) with sublimed sulfur (50 wt.%), and stirring;
5) superfine grinding: under the protection of inert gas, the toosendan fruit compound powder 3 with the powder granularity D90 being less than or equal to 23 microns is obtained through superfine grinding.
Example 5:
and (3) preparing the szechwan chinaberry fruit compound powder 4:
1) primary treatment: cleaning fructus Toosendan, and sun drying;
2) primary crushing: adding the primarily treated fructus toosendan into a grinder for primary grinding;
3) sieving: putting the primarily crushed szechwan chinaberry fruit into a vibrating screen (60 meshes) and screening;
4) mixing: mixing fructus Toosendan (10 wt.%) with sublimed sulfur (90 wt.%), and stirring;
5) superfine grinding: under the protection of inert gas, the toosendan fruit compound powder 4 with the powder granularity D90 being less than or equal to 23 microns is obtained by superfine grinding.
Example 6:
and (3) preparing the szechwan chinaberry fruit compound powder 4:
1) primary treatment: cleaning fructus Toosendan, and sun drying;
2) primary crushing: adding the primarily treated fructus toosendan into a grinder for primary grinding;
3) sieving: sieving the primarily crushed fructus toosendan with a 70-mesh sieve;
4) mixing: mixing fructus Toosendan (1 wt.%) with sublimed sulfur (99 wt.%), and stirring;
5) superfine grinding: under the protection of inert gas, the toosendan fruit compound powder with the powder granularity D90 being less than or equal to 23 microns is obtained through superfine grinding.
Example 7:
according to the guiding principle of field test for evaluating pesticide effect of acaricide for bees, the prevention and treatment effect of the small bee mites is evaluated on the szechwan chinaberry fruit powder and the szechwan chinaberry fruit compound powder provided by the invention.
The test method comprises the following steps:
the test bee colony is provided by Wang animal health Limited liability company test bee farm in Sichuan, and selected bee colonies with consistent colony potential (6 spleens) and small bee mite parasitism level are divided into 8 groups, wherein each group comprises 20 groups, namely a test group 1 (szechwan chinaberry fruit powder 1), an experiment group 2 (szechwan chinaberry fruit compound powder 1), an experiment group 3 (szechwan chinaberry fruit compound powder 2), an experiment group 4 (szechwan chinaberry fruit compound powder 3), an experiment group 5 (szechwan chinaberry fruit compound powder 4), an experiment group 6 (szechwan chinaberry fruit compound powder 5), a positive control group 7 (sublimed sulfur group) and a negative control group 8 (no medicine), and the bee species are Italian bees.
The medication method comprises the following steps: the bee colony is applied once every 7 days, three times in one period, and the amitraz is used for spraying the spleen after 21 days.
After the start of the test, the collection floor was checked daily for house mites, the colonies were identified before the start of the test (eggs, larvae, bee caps, adult bees (foot boxes) were recorded, respectively) on days 22 and 43, and the brood mite infestations in the caps were checked before the start of the test, on days 22 and 43.
Parasitic rate of wasp mites in the closure: randomly picking 100 sealing covers from different son-spleen areas in the bee colony by using tweezers, clamping bee larvae or pupae in the sealing cover rooms, and counting the number of the sealing cover rooms with the small bee mites.
The parasitic rate (%) of the small bee mites in the cap (number of covered rooms with mites/total number of covered rooms examined) x 100%
Calculating the mite-dropping rate:
the number of mites falling: after administration, the mite-dropping plate was observed once a day, the number of mites (a) was collected and counted, and the total number of mites (Σ a) at the end of the test was counted
Counting the number of the final mites: at the end of the test, each test group bee colony is sprayed with excess amitraz, and the number of the final mites (b) is counted. The number (b) is added with the total mite number (sigma a) of each bee colony to be used as the total mite number of the bee colony, and the mite falling rate of each test group bee colony is calculated respectively.
And (3) test results:
the mite shedding rate results are given in the following table:
| item | Average mite-falling rate% |
| Toosendan fruit powder 1 | 96.7 |
| Fructus Toosendan compound powder 1 | 97.3 |
| Fructus Toosendan compound powder 2 | 97.0 |
| Fructus Toosendan compound powder 3 | 96.8 |
| Fructus Toosendan compound powder 4 | 96.1 |
| Fructus Toosendan compound powder 5 | 95.9 |
| Sublimed sulphur | 95.6 |
| Negative control | 18.3 |
The parasitic rate results are shown in the following table:
the results of the group potential survey are shown in the following table:
and (4) conclusion:
according to the field test guiding principle of evaluating the pesticide effect of the acaricide for bees, the evaluation of the control effect of the small bee mites is carried out on the szechwan chinaberry fruit powder and the szechwan chinaberry fruit compound powder provided by the invention, and the results are as follows: the average mite-killing rate and the parasitic rate of the szechwan chinaberry fruit powder and the szechwan chinaberry fruit compound powder are superior to those of the sublimed sulfur powder, and the result is superior to that of the sublimed sulfur powder in terms of the swarm vigor. Therefore, the chinaberry fruit powder and the chinaberry fruit compound powder have good prevention and treatment effects on bee mites and are safe to bees.
The above description is only a preferred embodiment of the present invention, and is not intended to limit the present invention in any way, and all simple modifications and equivalent variations of the above embodiments according to the technical spirit of the present invention are included in the scope of the present invention.
Claims (2)
1. The application of the toosendan fruit for preventing and treating bee mites is characterized in that the toosendan fruit powder is applied to the prevention and treatment of the bee mites, and the preparation method of the toosendan fruit powder comprises the following steps:
1) primary treatment: cleaning fructus Toosendan, and sun drying;
2) primary crushing: adding the primarily treated fructus toosendan into a grinder for primary grinding;
3) sieving: sieving the primarily pulverized fructus Toosendan with 50-70 mesh sieve;
4) superfine grinding: the toosendan fruit powder with the powder granularity D90 less than or equal to 23 microns is obtained by superfine grinding.
2. The use of fructus Toosendan for preventing and treating bee mites as claimed in claim 1, wherein the powder is applied by spreading bee channels and frame beams, or directly applied on sealed spleens.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710582870.8A CN107307012B (en) | 2017-07-17 | 2017-07-17 | Application of szechwan chinaberry fruit in preventing and treating bee mites |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710582870.8A CN107307012B (en) | 2017-07-17 | 2017-07-17 | Application of szechwan chinaberry fruit in preventing and treating bee mites |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN107307012A CN107307012A (en) | 2017-11-03 |
| CN107307012B true CN107307012B (en) | 2020-05-08 |
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