CN103006672A - Vitamin-D3-contained filling agent for preventing dairy cow mastitis and preparation method of filling agent - Google Patents
Vitamin-D3-contained filling agent for preventing dairy cow mastitis and preparation method of filling agent Download PDFInfo
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Abstract
The invention provides a vitamin-D3-contained filling agent for preventing dairy cow mastitis and a preparation method of the filling agent. Every 10g of filling agent is prepared from the following materials by weight: 20mg-50mg of vitamin D3, 50mg-250mg of ceftiofur hydrochloride, 0.65g-2g of thickener, 50mg-100mg of antioxidant, and the balance of soybean oil or liquid paraffin. The preparation method of the vitamin-D3-contained filling agent comprises the following steps of: preparing the materials for future use; mixing the vitamin D3 and the ceftiofur hydrochloride, dispersing the obtained mixture by the soybean oil or the liquid paraffin, and ultrasonically stirring the mixture with the soybean oil or the liquid paraffin to uniformly disperse the mixture to obtain active ingredient mixed liquor; heating up residual solvent, and adding the thickener and the antioxidant into the mixture to obtain accessory mixed liquor; pouring the active ingredient mixed liquor into the accessory mixed liquor, uniformly stirring the mixture to obtain the vitamin-D3-contained filling agent for preventing the dairy cow mastitis. The vitamin-D3-contained filling agent is used for preventing and curing the dairy cow mastitis caused by pathogenic bacteria such as staphylococcus aureus, streptococcus, escherichia coli and salmonella.
Description
Technical field
The invention belongs to the veterinary medicine field, relate to filling agent of a kind of control mammitis of cow that contains vitamin D3 and preparation method thereof.
Background technology
Vitamin D3 belongs to the steroid derivant, and its active metabolite in vivo has 25 (OH) D3,1,25 (OH)
2D3, the vitamin D3 metabolic conversion is 25 (OH) D3,25 (OH) D3 is 1,25 (OH) through l α-further metabolic conversion of hydroxylase effect
2D3.Studies show that 1,25 (OH)
2D3 is the most important active metabolite of vitamin D3, is not only Ca
2+The main regulatory factors of stable state, and can participate in immune differentiation and adjusting.
At present; the antibiotherapy of cephalosporin or penicillins is adopted in the treatment of mammitis of cow mostly; but this therapy is because the antibiotic using dosage is large; at least the antibiotic that contains 300mg in every 10g medicine; in cure diseases, but reduced the immunity of organisms of milch cow, made the milch cow body produce dependency to antibiotic, in addition; the long-term a large amount of antibiotic that use finally also can cause having in the milk of output antibiotic residual.
Summary of the invention
The objective of the invention is for above-mentioned problems of the prior art, filling agent of a kind of control mammitis of cow that contains vitamin D3 and preparation method thereof is provided.
The technical solution adopted in the present invention is: a kind of filling agent that contains the control mammitis of cow of vitamin D3, every 10g filling agent is made by the raw material of following weight ratio: vitamin D3 20-50mg, Ceftiofur Hydrochloride 50-250mg, thickening agent 0.65-2g, antioxidant 50-100mg, surplus soybean oil or liquid paraffin.
Described thickening agent is Sargassum polysaccharides or glyceryl monostearate.
Described antioxidant is any in dibenzylatiooluene, BHA, the propyl gallate.
Every 10g filling agent contains Ceftiofur Hydrochloride 100-150mg.
A kind of filling agent that contains the control mammitis of cow of vitamin D3, every 10g filling agent is made by the raw material of following weight ratio: vitamin D3 35mg, Ceftiofur Hydrochloride 125mg, thickening agent Sargassum polysaccharides 1.32g, antioxidant dibenzylatiooluene 75mg, surplus is soybean oil.
A kind of filling agent that contains the control mammitis of cow of vitamin D3, every 10g filling agent is made by the raw material of following weight ratio: vitamin D3 50mg, Ceftiofur Hydrochloride 150mg, thickening agent glyceryl monostearate 0.65g, antioxidant BHA 100mg, surplus is liquid paraffin.
The preparation method of the filling agent of the control mammitis of cow that contains vitamin D3 of the present invention is as follows:
(1) get vitamin D3 20-50mg, Ceftiofur Hydrochloride 50-250mg, thickening agent 0.65-2g, antioxidant 50-100mg, soybean oil or liquid paraffin are supplied 10g, and be for subsequent use;
(2) vitamin D3, Ceftiofur Hydrochloride are mixed after, disperse with soybean oil or the liquid paraffin of 1/3 dosage, ultrasonic agitation 43-47min extremely is uniformly dispersed fully, obtains the effective ingredient mixed liquor;
Soybean oil or the liquid paraffin that (3) will remain 2/3 dosage are heated to 65-80 ℃, then successively to wherein adding thickening agent, antioxidant, are stirred to fully dissolving, are cooled to 30 ℃, obtain the adjuvant mixed liquor;
(4) the effective ingredient mixed liquor is poured in the adjuvant mixed liquor, stirred, then stir 30-50min with high-shear emulsion machine, obtain the filling agent that 10g contains the control mammitis of cow of vitamin D3 after placement is cooled to room temperature.
The preparation method of the filling agent of the control mammitis of cow that contains vitamin D3 of the present invention is as follows:
(1) get vitamin D3 35mg, Ceftiofur Hydrochloride 125mg, thickening agent Sargassum polysaccharides 1.32g, antioxidant dibenzylatiooluene 75mg, soybean oil 8.45g, for subsequent use;
(2) vitamin D3, Ceftiofur Hydrochloride are mixed after, disperse with the soybean oil of 2.82g, ultrasonic agitation 45min extremely is uniformly dispersed fully, obtains the effective ingredient mixed liquor;
The soybean oil that (3) will remain 5.63g is heated to 72.5 ℃, and then successively to wherein adding thickening agent Sargassum polysaccharides 1.32g, antioxidant dibenzylatiooluene 75mg is stirred to fully dissolving, is cooled to 30 ℃, obtains the adjuvant mixed liquor;
(4) the effective ingredient mixed liquor is poured in the adjuvant mixed liquor, stirred, then stir 40min with high-shear emulsion machine, obtain the filling agent that 10g contains the control mammitis of cow of vitamin D3 after placement is cooled to room temperature.
After ill milch cow perfusion the present invention being contained the filling agent of control mammitis of cow of vitamin D3, cow breast is subject to antibacterial and infects, can be by TLR pathway activation cattle mononuclear cell and macrophage, and then the expression of rise l α-'-hydroxylase gene, and the expression of l α-'-hydroxylase gene can cause 25 (OH) D3 to change into 1,25 (OH)
2D3,1,25 (OH)
2D3 can induce activation antimicrobial peptide-antibacterial peptide again, and then suppresses the breeding of pathogen.Vitamin D3 and Ceftiofur Hydrochloride can play synergistic function in Contents in Cows, obviously improve the inhibition ability to pathogen.This filling agent can further be repaired the mammary system of ill milch cow.
A kind of filling agent that contains the control mammitis of cow of vitamin D3 of the present invention can be used for prevention and treats by germ mammitis of cow such as staphylococcus aureus, streptococcus, escherichia coli and Salmonellas.Clinical type and recessive mammitis of cow there is significant therapeutic effect.Compared with prior art, this filling agent performance drug effect speed is fast, can significantly improve prevention and the therapeutic effect of mammitis of cow, improves the autoimmune ability of milch cow body in treatment, improves the milch cow quality, and cure rate reaches 86.1%, and effective percentage reaches 97.9%.
In addition; the vitamin D3 that a kind of filling agent that contains the control mammitis of cow of vitamin D3 of the present invention adds substitutes the antibiotic of doses; reduced antibiotic using dosage, efficiently solved because a large amount of use antibiotic cause the milch cow body and antibiotic is produced the problem of dependency and Residue of Antibiotics in Milk.
The specific embodiment
A kind of filling agent that contains the control mammitis of cow of vitamin D3 of the present invention, every 10g filling agent is made by the raw material of following weight ratio: vitamin D3 20-50mg, Ceftiofur Hydrochloride 50-250mg, thickening agent 0.65-2g, antioxidant 50-100mg, surplus soybean oil or liquid paraffin.
Preferred thickening agent is Sargassum polysaccharides or glyceryl monostearate.Selected thickening agent and vitamin D3 and Ceftiofur Hydrochloride all have optimum compatibility, can fully improve the viscosity of filling agent, guarantee the dosage form of filling agent of the present invention, keep the stable performance that filling agent pours into drug effect behind the cow breast.
Preferred antioxidant is any in antioxidant dibenzylatiooluene, propyl gallate, the BHA.Selected antioxidant has stronger antioxygen, bacteriostasis to vitamin D3 and Ceftiofur Hydrochloride, can guarantee that the active component vitamin D3 is not oxidized in a long time, make the filling agent product that contains the control mammitis of cow of vitamin D3 of the present invention have the long holding time.
Preferably, every 10g filling agent contains Ceftiofur Hydrochloride 100-150mg.
A kind of filling agent that contains the control mammitis of cow of vitamin D3, every 10g filling agent is made by the raw material of following weight ratio: vitamin D3 35mg, Ceftiofur Hydrochloride 125mg, thickening agent Sargassum polysaccharides 1.32g, antioxidant dibenzylatiooluene 75mg, surplus is soybean oil.The filling agent made from this preferred ingredient has optimum therapeutic effect to the treatment of ill milch cow.
A kind of filling agent that contains the control mammitis of cow of vitamin D3, every 10g filling agent is made by the raw material of following weight ratio: vitamin D3 50mg, Ceftiofur Hydrochloride 150mg, thickening agent glyceryl monostearate 0.65g, antioxidant BHA 100mg, surplus is liquid paraffin.The filling agent made from this preferred component has preferably preventive effect to dry cow.
The preparation method of the filling agent of a kind of control mammitis of cow that contains vitamin D3 of the present invention:
(1) get vitamin D3 20-50mg, Ceftiofur Hydrochloride 50-250mg, thickening agent 0.65-2g, antioxidant 50-100mg, soybean oil or liquid paraffin are supplied 10g, and be for subsequent use;
(2) vitamin D3, Ceftiofur Hydrochloride are mixed after, disperse with soybean oil or the liquid paraffin of 1/3 dosage, ultrasonic agitation 43-47min extremely is uniformly dispersed fully, obtains the effective ingredient mixed liquor;
Soybean oil or the liquid paraffin that (3) will remain 2/3 dosage are heated to 65-80 ℃, then successively to wherein adding thickening agent, antioxidant, are stirred to fully dissolving, are cooled to 30 ℃, obtain the adjuvant mixed liquor;
(4) the effective ingredient mixed liquor is poured in the adjuvant mixed liquor, stirred, then stir 30-50min with high-shear emulsion machine, obtain the filling agent that 10g contains the control mammitis of cow of vitamin D3 after placement is cooled to room temperature.
Above-mentioned preparation method process is simple, and easily operation can guarantee that raw material is well dispersed in soybean oil or the liquid paraffin, guarantees that the filling agent of the control mammitis of cow that this contains vitamin D3 can be brought into play rapidly drug effect after absorbing in the Contents in Cows.
The antibiotic that active component vitamin D3 in the filling agent of a kind of control mammitis of cow that contains vitamin D3 of the present invention also can be used for the treatment of with other one or more mammitis of cow makes up, and all can play preferably antiinflammatory, bacteriostasis.
The raw material of the filling agent of a kind of control mammitis of cow that contains vitamin D3 of the present invention can have following multiple combination mode:
1, vitamin D3 20mg, Ceftiofur Hydrochloride 50mg, thickening agent Sargassum polysaccharides 0.65g, antioxidant propyl gallate 50mg, soybean oil 9.23g.
2, vitamin D3 50mg, Ceftiofur Hydrochloride 250mg, thickening agent glyceryl monostearate 2g, antioxidant propyl gallate 100mg, liquid paraffin 7.6g.
3, vitamin D3 35mg, Ceftiofur Hydrochloride 125mg, thickening agent Sargassum polysaccharides 1.32g, antioxidant dibenzylatiooluene 75mg, soybean oil 8.44g.
4, vitamin D3 20mg, Ceftiofur Hydrochloride 150mg, thickening agent glyceryl monostearate 0.65g, antioxidant BHA 100mg, liquid paraffin 9.08g.
5, vitamin D3 50mg, Ceftiofur Hydrochloride 100mg, thickening agent glyceryl monostearate 2g, antioxidant BHA 70mg, liquid paraffin 7.78g.
The preparation method of the filling agent of a kind of control mammitis of cow that contains vitamin D3 of the present invention can have following specific embodiment:
The concrete preparation method of embodiment 1 is as follows:
(1) get vitamin D3 20mg, Ceftiofur Hydrochloride 50mg, thickening agent Sargassum polysaccharides 0.65g, antioxidant propyl gallate 50mg, soybean oil 9.23g, for subsequent use;
(2) after vitamin D3, Ceftiofur Hydrochloride mix, disperse with the liquid paraffin of 3.08g, ultrasonic agitation 43min extremely is uniformly dispersed fully, obtains the effective ingredient mixed liquor;
The soybean oil that (3) will remain 6.15g is heated to 65 ℃, then successively to wherein adding thickening agent Sargassum polysaccharides, antioxidant propyl gallate, is stirred to fully dissolving, is cooled to 30 ℃, obtains the adjuvant mixed liquor;
(4) the effective ingredient mixed liquor is poured in the adjuvant mixed liquor, stirred, then stir 30min with high-shear emulsion machine, obtain the filling agent that 10g contains the control mammitis of cow of vitamin D3 after placement is cooled to room temperature.
The concrete preparation method of embodiment 2 is as follows:
(1) get vitamin D3 50mg, Ceftiofur Hydrochloride 250mg, thickening agent glyceryl monostearate 2g, antioxidant propyl gallate 100mg, liquid paraffin 7.6g, for subsequent use;
(2) after vitamin D3, Ceftiofur Hydrochloride mix, disperse with the liquid paraffin of 2.53g, ultrasonic agitation 47min extremely is uniformly dispersed fully, obtains the effective ingredient mixed liquor;
The liquid paraffin that (3) will remain 5.07g is heated to 80 ℃, and then successively to wherein adding thickening agent glyceryl monostearate 2g, antioxidant propyl gallate 100mg is stirred to fully dissolving, is cooled to 30 ℃, obtains the adjuvant mixed liquor;
(4) the effective ingredient mixed liquor is poured in the adjuvant mixed liquor, stirred, then stir 50min with high-shear emulsion machine, obtain the filling agent that 10g contains the control mammitis of cow of vitamin D3 after placement is cooled to room temperature.
The concrete preparation method of embodiment 3 is as follows:
(1) get vitamin D3 35mg, Ceftiofur Hydrochloride 125mg, thickening agent Sargassum polysaccharides 1.32g, antioxidant dibenzylatiooluene 75mg, soybean oil 8.44g, for subsequent use;
(2) vitamin D3, Ceftiofur Hydrochloride are mixed after, disperse with the soybean oil of 2.81g, ultrasonic agitation 45min extremely is uniformly dispersed fully, obtains the effective ingredient mixed liquor;
The soybean oil that (3) will remain 5.63g is heated to 72.5 ℃, and then successively to wherein adding thickening agent Sargassum polysaccharides 1.32g, antioxidant dibenzylatiooluene 75mg is stirred to fully dissolving, is cooled to 30 ℃, obtains the adjuvant mixed liquor;
(4) the effective ingredient mixed liquor is poured in the adjuvant mixed liquor, stirred, then stir 40min with high-shear emulsion machine, obtain the filling agent that 10g contains the control mammitis of cow of vitamin D3 after placement is cooled to room temperature.
The concrete preparation method of embodiment 4 is as follows:
(1) get vitamin D3 20mg, Ceftiofur Hydrochloride 150mg, thickening agent glyceryl monostearate 0.65g, antioxidant BHA 100mg, liquid paraffin 9.08g, for subsequent use;
(2) vitamin D3, Ceftiofur Hydrochloride are mixed after, disperse with the liquid paraffin of 3.03g, ultrasonic agitation 45min extremely is uniformly dispersed fully, obtains the effective ingredient mixed liquor;
The liquid paraffin that (3) will remain 6.05g is heated to 78 ℃, then successively to wherein adding thickening agent glyceryl monostearate, antioxidant BHA, is stirred to fully dissolving, is cooled to 30 ℃, obtains the adjuvant mixed liquor;
(4) the effective ingredient mixed liquor is poured in the adjuvant mixed liquor, stirred, then stir 50min with high-shear emulsion machine, obtain the filling agent that 10g contains the control mammitis of cow of vitamin D3 after placement is cooled to room temperature.
The concrete preparation method of embodiment 5 is as follows:
(1) get vitamin D3 50mg, Ceftiofur Hydrochloride 100mg, thickening agent glyceryl monostearate 2g, antioxidant BHA 70mg, liquid paraffin 7.78g, for subsequent use;
(2) vitamin D3, Ceftiofur Hydrochloride are mixed after, disperse with the liquid paraffin of 2.59g, ultrasonic agitation 45min extremely is uniformly dispersed fully, obtains the effective ingredient mixed liquor;
The liquid paraffin that (3) will remain 5.19g is heated to 78 ℃, then successively to wherein adding thickening agent glyceryl monostearate, antioxidant BHA, is stirred to fully dissolving, is cooled to 30 ℃, obtains the adjuvant mixed liquor;
(4) the effective ingredient mixed liquor is poured in the adjuvant mixed liquor, stirred, then stir 50min with high-shear emulsion machine, obtain the filling agent that 10g contains the control mammitis of cow of vitamin D3 after placement is cooled to room temperature.
Filling agent to the control mammitis of cow that contains vitamin D3 made with the embodiment of the invention carries out following clinical trial:
One, mammitis of milk cow in lactation period clinical experimental study
1, test material:
Large-scale cattle farm, periphery one place, Huhehaote City, the Inner Mongol.Have 620 lactation period the clinical type Isolated from Bovine Mastitis, 639 ill breast districts.The ill breast district of milch cow increases, occurs red, swollen serious, palpation heat, significant reaction bitterly, lactation amount falls sharply, even the dry up, the milk character is unusual, and floccule, grumeleuse, extract or the blood streak are arranged, and obvious abnormal flavour is arranged, look cold and detached, searches for food seldom.
2, test method:
620 cow heads of suffering from mammitis of cow are divided into three groups at random:
| Group | Case load (ill breast district number) | Medicine |
| One group of lactation period | 207（213） | The homemade Ceftiofur Hydrochloride folk prescription of the present patent application person filling agent (the hydrochloric ceftiofur 300mg of every 10g filling agent) |
| Two groups of lactation periods | 207（213） | Filling agent (every 10g filling agent contains vitamin D3 20mg, Ceftiofur Hydrochloride 50mg) by the control mammitis of cow that contains vitamin D3 of the embodiment of the invention 1 preparation |
| Three groups of lactation periods | 206（213） | Filling agent (every 10g filling agent contains vitamin D3 50mg, Ceftiofur Hydrochloride 250mg) by the control mammitis of cow that contains vitamin D3 of the embodiment of the invention 2 preparation |
Sick cattle early, evening, each infected the breast district with sterilization towel cleaning first, squeezes and removes milk, soaks each nipple approximately 30 seconds with 0.15% povidone iodine Baokang medicinal liquid again when milking, and then carried out perfusion administration in the nipple.During perfusion pastille is injected tube head and insert nipple, pushing piston is slowly injected medicine in the breast gently, massages gently subsequently corresponding breast district, and medicine is evenly distributed.Inject respectively corresponding medicine when each organizes medication, inject 1 (10g/ props up) at every turn, every day 2 times (interval 12 hours), be used in conjunction 3 days.Last administration is after 7 days, and comprehensive coherence check result is by following standard determination curative effect of medication.
3, curative effect determinate standard:
(1) cure: sick cattle spirit, appetite recover normal, the lactation amount rebound significantly; Medication breast district is red, swollen, hot, the pain symptom is eliminated; It is normal that the milk character is recovered, milk SCC(somatic number)＜500,000/ml; Main pathogen is eliminated.
Cure rate=(curing breast district number/group innerlich anwenden breast district number in the group) * 100%
(2) effective: sick cattle spirit, appetite recover normal, and lactation amount has rise; Medication breast district is red, swollen, hot, the pain symptom is clearly better; The unusual character of milk has recovery, and milk SCC is between 50 ~ 1,000,000/ml; Main pathogen is eliminated.
Effective percentage=(effectively newborn district number in the group/group innerlich anwenden breast district number) * 100%
4, Comparison of therapeutic:
(1) remission contrast:
The clinical result of use situation of mammitis of milk cow in lactation period case contrasts such as following table:
(2) somatic cell from milk number (SCC) testing result after the district's treatment of the ill breast of milch cow:
After the last administration the 7th day, gather each newborn sample in treatment breast district, squeeze out first first three milk during sampling, squeeze subsequently and get approximately 30ml as detecting newborn sample (50ml sterilizing test tubes dress).SCC detects and adopts integrated milk analysis instrument (MSC5000) to carry out result such as following table:
Before the medication, the somatic cell from milk number average in all ill breast districts＞1,000,000/mL.After the medication, the SCC in all treatment breast districts all significantly descends.
(3) clinical therapeutic efficacy of mammitis of milk cow in lactation period
Comprehensive above-mentioned testing result, statistics effective percentage and cure rate the results are shown in following table:
| Group | Case load (breast district number) | Effective percentage (%) | Cure rate (%) |
| One group of lactation period | 207（213） | 84.5（180/213） | 76.1（162/213） |
| Two groups of lactation periods | 207（213） | 88.3（188/213） | 77.5（165/213） |
| Three groups of lactation periods | 206（213） | 95.8（204/213） | 83.1（177/213） |
5, conclusion
Above-mentioned result of the test shows, tested filling agent all has scavenging action to the pathogen for the treatment of the breast district, improves the clinical symptoms of mammitis of cow, reduces the somatic number of milk.But a kind of filling agent that contains the control mammitis of cow of vitamin D3 by the embodiment of the invention 1,2 preparations can effectively promote the therapeutic effect of mammitis of milk cow in lactation period, improves effective percentage and cure rate, improves the milch cow quality.
Two, the clinical experimental study of dry cow mastitis
1, test material
Choose Baotou large-scale milk cattle cultivating factory and do 582 of breast phase recessive mammitis of cow natural occurrence cases.
2, test method
Case is divided into three groups at random, grouping situation such as following table:
| Group | The case number | Medicine |
| Do one group of breast phase | 194 | The homemade Ceftiofur Hydrochloride folk prescription of the present patent application person filling agent (the hydrochloric ceftiofur 300mg of every 10g filling agent) |
| Do two groups of breast phases | 194 | Filling agent (every 10g filling agent contains vitamin D3 35mg, Ceftiofur Hydrochloride 125mg) by the control mammitis of cow that contains vitamin D3 of the embodiment of the invention 3 preparation |
| Do three groups of breast phases | 194 | Filling agent (every 10g filling agent contains vitamin D3 20mg, Ceftiofur Hydrochloride 150mg) by the control mammitis of cow that contains vitamin D3 of the embodiment of the invention 4 preparation |
By group above-mentioned filling agent is injected respectively in every breast of doing ill milch cow of breast phase, one of above-mentioned filling agent (10g/ props up) is injected in each newborn chamber, and be administered once every day, administration rear enclosed nipple, successive administration 3 days.
Respectively carry out a SCC and detect and BMT(mammitis of cow diagnostic reagent test is forward and backward doing breast phase ill milch cow) detection.The used newborn sample acquisition time of detection before the test is ill milch cow milking for the last time before the dried breast phase; After the used newborn sample acquisition time of detection after the test is ill milch cow calving.When gathering newborn sample, first cleaned, sterilize in each breast district, under the aseptic condition, discard first three milk, then gather newborn sample.
The SCC detection method is identical with the detection method of above-mentioned lactation period of ill milch cow.
The BMT detection method is: subclinical mastitis quick diagnosis liquid (stock solution) BMT by the commercial channel obtains, use the 1:4 distilled water diluting.Newborn sample is placed the diagnosis dish, and diagnosis dish inclination miter angle discards unnecessary newborn sample, and each newborn ware stays 2ml breast sample in the diagnosis dish.Add the BMT diagnosticum after 2ml dilutes in each sample, do horizontal sample concentric circular and shake, upper and lower inclination is judged by following standard after shaking 50s.
3, curative effect determinate standard:
Degree by gel reaction after the BMT diagnosticum mixes with newborn sample is divided into following five grades:
1. negative (-): somatic number is 0-20 ten thousand/ml in the milk, and reacting substance is even, flows without unusual, and it is yellow that color is more.
2. doubtful (±): ten thousand/ml of somatic cell numerical digit 20-50 in the milk, inclination diagnosis dish reactive flow, the batt layer of having tried to get to the heart of a matter occurs, and color mostly is yellow or green is slightly arranged, and is judged to be suspicious.
3. positive (+): somatic number is ten thousand/ml of 50-150 in the milk.Shake visible material inhomogeneous when ten thousand/ml of 50-80, mixture obviously has cotton-shapedly during pitch, and gelling thing reaction can be judged to be+1; As ten thousand of 80-150/when ml shakes, reactant thick middle wadding does not shake and separates, can be judged to be+2.Color is yellow green, and this breast district is latent mammitis.
4. weak positive (++): somatic number is ten thousand/ml of 150-500 in the milk, and reactant is the thickness hanging end when shaking, and color mostly is yellow green.
5. strong positive (+++): somatic number is in the milk〉5,000,000/ml, be block jelly when shaking reactant, the edge is translucent, color pale green or dark green.
Recovery from illness: after the milch cow calving, BMT detects negative (-).
Effectively: after the milch cow calving, BMT detects, and alleviates to some extent by the intensity grade of gel reaction.
Invalid: after the milch cow calving, BMT detects, and the intensity grade of pressing gel reaction is unchanged.
4, Comparison of therapeutic:
The detection statistics result who does breast phase three groups of ill milch cows contrasts situation such as following table:
5, conclusion
Above-mentioned result of the test shows, tested filling agent all has scavenging action to the pathogen in ill breast district, reduces the somatic number in the dairy cow milk.But the filling agent of the control mammitis of cow that contains vitamin D3 of making by the embodiment of the invention 3,4 can obviously improve the therapeutic effect of dry cow mastitis case.The filling agent that contains the control mammitis of cow of vitamin D3 of the present invention can effectively be treated and be done recessive mammitis of cow of breast phase, and new infection during the breast phase is done in prevention, significantly reduces the sickness rate of next lactational mammitis of cow.
Claims (8)
1. the filling agent that contains the control mammitis of cow of vitamin D3, it is characterized in that: every 10g filling agent is made by the raw material of following weight ratio: vitamin D3 20-50mg, Ceftiofur Hydrochloride 50-250mg, thickening agent 0.65-2g, antioxidant 50-100mg, surplus soybean oil or liquid paraffin.
2. the filling agent that contains the control mammitis of cow of vitamin D3 according to claim 1, it is characterized in that: described thickening agent is Sargassum polysaccharides or glyceryl monostearate.
3. the filling agent that contains the control mammitis of cow of vitamin D3 according to claim 1, it is characterized in that: described antioxidant is any in dibenzylatiooluene, BHA, the propyl gallate.
4. the filling agent that contains the control mammitis of cow of vitamin D3 according to claim 1, it is characterized in that: every 10g filling agent contains Ceftiofur Hydrochloride 100-150mg.
5. the filling agent that contains the control mammitis of cow of vitamin D3 according to claim 1, it is characterized in that: every 10g filling agent is made by the raw material of following weight ratio: vitamin D3 35mg, Ceftiofur Hydrochloride 125mg, thickening agent Sargassum polysaccharides 1.32g, antioxidant dibenzylatiooluene 75mg, surplus is soybean oil.
6. the filling agent that contains the control mammitis of cow of vitamin D3 according to claim 1, it is characterized in that: every 10g filling agent is made by the raw material of following weight ratio: vitamin D3 50mg, Ceftiofur Hydrochloride 150mg, thickening agent glyceryl monostearate 0.65g, antioxidant BHA 100mg, surplus is liquid paraffin.
7. the preparation method of the filling agent of the described control mammitis of cow that contains vitamin D3 according to claim 1-6, it is characterized in that: preparation method is as follows:
(1) get vitamin D3 20-50mg, Ceftiofur Hydrochloride 50-250mg, thickening agent 0.65-2g, antioxidant 50-100mg, soybean oil or liquid paraffin are supplied 10g, and be for subsequent use;
(2) vitamin D3, Ceftiofur Hydrochloride are mixed after, disperse with soybean oil or the liquid paraffin of 1/3 dosage, ultrasonic agitation 43-47min extremely is uniformly dispersed fully, obtains the effective ingredient mixed liquor;
Soybean oil or the liquid paraffin that (3) will remain 2/3 dosage are heated to 65-80 ℃, then successively to wherein adding thickening agent, antioxidant, are stirred to fully dissolving, are cooled to 30 ℃, obtain the adjuvant mixed liquor;
(4) the effective ingredient mixed liquor is poured in the adjuvant mixed liquor, stirred, then stir 30-50min with high-shear emulsion machine, obtain the filling agent that 10g contains the control mammitis of cow of vitamin D3 after placement is cooled to room temperature.
8. the preparation method of the filling agent of the control mammitis of cow that contains vitamin D3 according to claim 1, it is characterized in that: preparation method is as follows:
(1) get vitamin D3 35mg, Ceftiofur Hydrochloride 125mg, thickening agent Sargassum polysaccharides 1.32g, antioxidant dibenzylatiooluene 75mg, soybean oil 8.45g, for subsequent use;
(2) vitamin D3, Ceftiofur Hydrochloride are mixed after, disperse with the soybean oil of 2.82g, ultrasonic agitation 45min extremely is uniformly dispersed fully, obtains the effective ingredient mixed liquor;
The soybean oil that (3) will remain 5.63g is heated to 72.5 ℃, and then successively to wherein adding thickening agent Sargassum polysaccharides 1.32g, antioxidant dibenzylatiooluene 75mg is stirred to fully dissolving, is cooled to 30 ℃, obtains the adjuvant mixed liquor;
(4) the effective ingredient mixed liquor is poured in the adjuvant mixed liquor, stirred, then stir 40min with high-shear emulsion machine, obtain the filling agent that 10g contains the control mammitis of cow of vitamin D3 after placement is cooled to room temperature.
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| Application Number | Priority Date | Filing Date | Title |
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| CN2012105738499A CN103006672A (en) | 2012-12-26 | 2012-12-26 | Vitamin-D3-contained filling agent for preventing dairy cow mastitis and preparation method of filling agent |
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| CN2012105738499A CN103006672A (en) | 2012-12-26 | 2012-12-26 | Vitamin-D3-contained filling agent for preventing dairy cow mastitis and preparation method of filling agent |
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|---|---|---|---|
| CN2012105738499A Pending CN103006672A (en) | 2012-12-26 | 2012-12-26 | Vitamin-D3-contained filling agent for preventing dairy cow mastitis and preparation method of filling agent |
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| Country | Link |
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| CN (1) | CN103006672A (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104042619A (en) * | 2014-01-24 | 2014-09-17 | 临沂大学 | Ceftiofur hydrochloride cream and preparation method thereof |
| CN104546704A (en) * | 2013-12-10 | 2015-04-29 | 中国农业科学院饲料研究所 | Ceftiofur hydrochloride breast injection for dairy cow in dry period and preparation method thereof |
| CN105943541A (en) * | 2016-06-27 | 2016-09-21 | 齐鲁动物保健品有限公司 | Long-acting breast injection agent for treating cow mastitis in dry period and preparation method of long-acting breast injection agent |
-
2012
- 2012-12-26 CN CN2012105738499A patent/CN103006672A/en active Pending
Non-Patent Citations (1)
| Title |
|---|
| 李振: "日粮微量组分对奶牛乳房炎的调控作用", 《反刍动物营养》 * |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104546704A (en) * | 2013-12-10 | 2015-04-29 | 中国农业科学院饲料研究所 | Ceftiofur hydrochloride breast injection for dairy cow in dry period and preparation method thereof |
| CN104546704B (en) * | 2013-12-10 | 2017-04-05 | 中国农业科学院饲料研究所 | A kind of milk cow dry breast phase Ceftiofur Hydrochloride breast injection and preparation method thereof |
| CN104042619A (en) * | 2014-01-24 | 2014-09-17 | 临沂大学 | Ceftiofur hydrochloride cream and preparation method thereof |
| CN105943541A (en) * | 2016-06-27 | 2016-09-21 | 齐鲁动物保健品有限公司 | Long-acting breast injection agent for treating cow mastitis in dry period and preparation method of long-acting breast injection agent |
| CN105943541B (en) * | 2016-06-27 | 2019-02-12 | 齐鲁动物保健品有限公司 | For treating the long-acting breast injection and preparation method thereof of milk cow dry breast phase mammitis |
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Application publication date: 20130403 |