CN105031375B - For treating the medicine of mastitis for milk cows and preparation method thereof and detection method and purposes - Google Patents
For treating the medicine of mastitis for milk cows and preparation method thereof and detection method and purposes Download PDFInfo
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Abstract
The present invention relates to the field of Chinese medicines, be specifically related to a kind of medicine for treating mastitis for milk cows and preparation method thereof and detection method and purposes.This medicine is made up of the raw material of following weight portion: purple Pueraria lobota 1～2 weight portion, Panicum repens 1～2 weight portion;Purslane 0.5～1 weight portion.This medicine is prepared as tablet, pill, powder, hard capsule, soft capsule, granule.Preparation method is: the purple Pueraria lobota that is dried, Panicum repens, purslane, for the first time adds 11 times amount water soaking 1 hour, decocts 1 hour, and second time adds 4 times amount soak by water 1 hour, merges decocting liquid, filters, filtrate concentration, dry, pulverize into fine powder, sieves, and powder is made in mixing.The chromatographic condition of drug quality detection method is: use HypersilDs chromatographic column；Flowing phase: ratio is methyl alcohol 0.15% phosphoric acid solution of 30:70；Detection wavelength: 200nm；Column temperature: 20 DEG C；Flow velocity: 1.0mL min‑1；Sample size: 10 μ L.Medicament composing prescription of the present invention is reasonable, result for the treatment of is notable.
Description
Technical field
The present invention relates to the field of Chinese medicines, be specifically related to a kind of for medicine treating mastitis for milk cows and preparation method thereof with
Purposes.
Background technology
Mastitis for milk cows, also known as mastadenitis of cow (bovine mastitis), is that cow mammary gland is by physics, chemistry, micro-life
Thing stimulates the one causing the symptoms such as breast tissue local microcirculation obstacle, mammary gland alveolus generation pathology, immune dysfunction multiple
Miscellaneous disease.Microorganism is the Etiological of mastitis for milk cows, has now been found that there are about 150 multiple pathogens can cause milk cow's milk
Fang Yan, based on common bacteria is with staphylococcus, streptococcus, EHEC, bacillus pyogenes, account for Isolated from Bovine Mastitis 90% with
On.
According to dairy association of world statistics, whole world mastitis for milk cows incidence is about 50%.External mastitis for milk cows is sent out
Sick rate is 25%～60%, and China is 20%～70%, and the part cattle farm incidence of disease is higher.After milk cow generation mammitis, body starts
Defense system causes the body cells such as leucocyte in milk to increase, and the output of milk declines, and milk Quality Down, severe patient is likely to cause milk
Ox oestruses minimizing.Research report, the sick milk cow of average every hair loses every year and surpasses 100 dollars, and entire United States is every year because of mastitis for milk cows
The loss causing is more than 2,000,000,000 dollars.The economic loss that the whole world causes because of mammitis every year is up to 35,000,000,000 dollars.
China is since the eighties in 20th century starts to study mastitis for milk cows, and mammitis always treated by antibiotic
Choice drug, wherein antibiotic and the chemicals such as penicillin, streptomysin, gentamicin, Cloxacillin Sodium, ceftiofur sodium
Treating bacillary mastitis for milk cows clinically and achieving significant effect, treatment rate is up to 90%.But, make in a large number for a long time
Result in the appearance of drug-fast bacteria, superbacteria with antibiotic, the residual metabolin serious threat of all kinds of chemosynthesis medicines
Human health.Currently, Safety of Food Quality gradually causes the attention of society, and researcher begins one's study novel green without public
Harmful, noresidue, quick-acting medicine comes substitute antibiotics and chemicals treatment mastitis for milk cows.Nuisanceless, noresidue, nothing
The Chinese medicine of off-drug period becomes current study hotspot.
Content of the invention
It is an object of the invention to for the deficiencies in the prior art, provide a kind of reasonable recipe, result for the treatment of good be used for control
Treat the medicine of mastitis for milk cows.
It is a further object of the present invention to provide the preparation method of this medicine.
Present invention also offers the quality determining method of this medicine.
Present invention also offers the pharmaceutical applications of this medicine.
Medicine of the present invention is meticulously to develop for many years through inventor, is mainly used in treating mastitis for milk cows.Medicine of the present invention
Can clearing heat and detoxicating, promoting blood circulation to remove blood stasis, breast blood circulation can be promoted again to dredge smooth, the absorption being conducive to inflammation swollen is dissipated, and promotes milk
Secretion, there is preferable result for the treatment of to mastitis for milk cows.
In medicine of the present invention, the Ji Yuan of bulk drug is as follows:
Purple Pueraria lobota is Vitaceae ampelopsis A.humulifoliavar. HeterophyllaAmpelopsis humulifolia Bungevar.heterophylla(Thunb.) K.Koch. [A.heterophylla(Thunb.) Sieb. et Zucc]Root
Skin.
Panicum repens is grass Panicum repensPanicum repens L.Herb.
Purslane is the herb of Portulacaceae Portulacaceae plant.
It is an object of the invention to be realized by the following manner:
A kind of medicine is made up of the raw material of following weight portion: purple Pueraria lobota 1～2 weight portion, Panicum repens 1～2 weight portion；Horse
Bitterroot 0.5～1 weight portion.
This medicine is preferably made up of the raw material of following weight portion: purple Pueraria lobota 1 weight portion, Panicum repens 2 weight portion purslane
0.5 weight portion.
This medicine is preferably made up of the raw material of following weight portion: purple Pueraria lobota 2 weight portion, Panicum repens 1 weight portion；Purslane
1 weight portion.
This medicine is preferably made up of the raw material of following weight portion: purple Pueraria lobota 1 weight portion, Panicum repens 1 weight portion；Purslane
0.8 weight portion.
Described medicine can be prepared as tablet, pill, powder, hard capsule, soft capsule, granule.
Described medicine is adopted and is prepared with the following method: takes purple Pueraria lobota, Panicum repens, purslane, mixing, adds 3～15 times amount
Water soaking 0.5～2 hour, decocts 0.5～2 hour, decocts 2～4 times, and 0.5～2 hour every time, extract merged, and filtered, filter
Liquid concentrates, and dry, pulverize into fine powder, adds auxiliary material, mixes, makes powder, to obtain final product.
Described medicine is adopted and is prepared with the following method: take dry purple Pueraria lobota, Panicum repens, purslane, adds 11 times amount for the first time
Water soaking 1 hour, decocts 1 hour, and second time adds 4 times amount soak by water 1 hour, merges decocting liquid, filters, and filtrate concentrates, and is dried,
It is ground into fine powder, sieves, mix, make powder, to obtain final product.
Described medicine is adopted and is detected with the following method: use high performance liquid chromatography to carry out the assay of salicin:
(1) chromatographic condition: use HypersilDs chromatographic column；Flowing phase: ratio be 20～40:60～80 methyl alcohol-
0.15% phosphoric acid solution；Detection wavelength: 190～210nm；Column temperature: 15～25 DEG C；Flow velocity: 0.5～1.5mL min-1；Sample size:
5～20 μ L；
(2) prepared by reference substance solution: it is appropriate that precision weighs the salicin comparison product being dried to constant weight, adds methyl alcohol dissolving and makes
Reference substance solution；
(3) preparation of need testing solution: precision weighs medicine of the present invention, adds methyl alcohol, is heated to reflux, extract reflux solvent
And be concentrated to dryness, residue is dissolved in water, and extracts with the shaking of water saturated n-butanol, merges n-butanol extracting liquid, washed by ammonia solution
Washing, n-butanol extracting liquid recycling design is to doing, and residue adds methyl alcohol and dissolves, and shakes up, and filters, takes filtrate, obtain need testing solution；
(4) measure: precision measures above-mentioned need testing solution, each 5～20 μ L of reference substance solution respectively, inject high-efficient liquid phase color
Spectrometer, detects.
Described medicine preferably employs the detection of following method: the content using high performance liquid chromatography to carry out salicin is surveyed
Fixed:
(1) chromatographic condition: use HypersilDs chromatographic column；Flowing phase: ratio is that methyl alcohol-0.15% phosphoric acid of 30:70 is molten
Liquid；Detection wavelength: 200nm；Column temperature: 20 DEG C；Flow velocity: 1.0mL min-1；Sample size: 10 μ L；
(2) prepared by reference substance solution: it is appropriate to the salicin comparison product of constant weight that precision weighs 80 DEG C of dryings, adds methyl alcohol and dissolves
Make the reference substance solution containing 0.2mg for every 1mL；
(3) preparation of need testing solution: precision weighs medicine 10g of the present invention, adds methyl alcohol 40mL, is heated to reflux 4h, extracts
Liquid reflux solvent is simultaneously concentrated to dryness, residue add water 10mL dissolve, with water saturated n-butanol shaking extract 5 times, each 20mL, conjunction
And n-butanol extracting liquid, washing 3 times with ammonia solution, each 15mL, n-butanol extracting liquid recycling design is to doing, and it is molten that residue adds methyl alcohol
Solve and be transferred in 10mL volumetric flask, adding methyl alcohol to scale, shake up, filtering, take filtrate, obtain need testing solution；
(4) measure: precision measures above-mentioned need testing solution, each 10 μ L of reference substance solution respectively, inject high performance liquid chromatography
Instrument, detects.
Described medicine can be used for preparation treatment mastitis for milk cows, the medicine of ox chordapsus or health products.
Experiment one: the experimental study of drug therapy mastitis for milk cows of the present invention
1 experiment material
1.1 test drug
Medicine of the present invention
Prescription: purple Pueraria lobota 50g, Panicum repens 50g, purslane 25g
Preparation method: take dry purple Pueraria lobota 50g, that Panicum repens 50g, purslane 25g add 1100mL water soaking 1 for the first time is little
When, decocting 1 hour, second time adds 400mL, decocts 1 hour, merges decocting liquid, filters, and filtrate concentrates, and is dried, obtains medicine of the present invention
Thing.
Drugs compared A
Prescription: purple Pueraria lobota 100g
Preparation method: take dry purple Pueraria lobota 100g, adds 1100mL water soaking 1 hour for the first time, decocts 1 hour, for the second time
Adding 400mL, decocting 1 hour, merge decocting liquid, filter, filtrate concentrates, and is dried, obtains drugs compared A.
Drugs compared B
Prescription: Panicum repens 100g
Preparation method: take dry Panicum repens 100g, adds 1100mL water soaking 1 hour for the first time, decocts 1 hour, and second
The secondary 400mL that adds, decocts 1 hour, merges decocting liquid, filters, and filtrate concentrates, and is dried, obtains drugs compared B.
1.2 experimental animal
The Cloud Terrace farm, Lianyungang cattle farm 400 lactating cow, the milk cow declining the output of milk carries out California mammitis
Detection method (CMT) detects, and chooses the positive milk cow of more than 90 " ++ ", is randomly divided into three groups, medication therapy groups i.e. of the present invention, right
Ratio medicine A treatment group, drugs compared B treatment group, often organize 30.
1.3 test equipment
Bao Dinglan, Baoding rope, dispensing bottle, 50mL syringe, No. 20 logical breast pins, California mammitis detection method (CMT) inspections
Plate.
2 test methods
2.1 California mammitis detection methods
The CMT diagnosticum of the milk and dilution of drawing Isodose respectively adds inspection panel, fully mixes, observes.
California mammitis detection method criterion is as described in kit specification, and mastitis for milk cows result for the treatment of criterion is shown in Table 1.
Table 1 mastitis for milk cows result for the treatment of criterion
2.2 clinical packets are administered
Medication therapy groups of the present invention: medicine 10g of the present invention, is soaked by boiling water, stirs, carry out with dispensing bottle after cooling
Gavaging, each 100mL, 1 time/d, being used in conjunction 5d is a course for the treatment of.
Drugs compared A treatment group: drugs compared A 10g, is soaked by boiling water, stirs, carry out with dispensing bottle after cooling
Gavaging, each 100mL, 1 time/d, being used in conjunction 5d is a course for the treatment of.
Drugs compared B group: drugs compared B 10g, is soaked by boiling water, stirs, gavage with dispensing bottle after cooling,
Each 100mL, 1 time/d, being used in conjunction 5d is a course for the treatment of.
For test milk cow, carrying out unified feeding and management, unified feed formula, different pharmaceutical treats three courses for the treatment of,
Amount to 15d.After three courses for the treatment of, CMT detection is carried out to three groups of milk cows.
3 results
The positive milk cow of 3.1 mammitises
400 cows in milk are detected by application California mammitis detection method, the positive milk cow more than " ++ " 150, sun
Property rate reach 37.5%, wherein have red, swollen, hot, 100 of pain shape, account for positive milk cow 66.7%, 100 chosen 90 and does and face
Bed test.
3.2 different pharmaceutical results for the treatment of
After three courses for the treatment of, CMT detection is carried out to the result for the treatment of of three groups of milk cows, and whether recurs in reinspection in the 20th day, knot
Fruit is shown in Table 2.The 65.2%th, the cure rate of medicine group of the present invention, drugs compared A group and drugs compared B group mastitis for milk cows is respectively
38.2%th, 41.1%, efficient is the 97.8%th, the 74.1%th, 85.5% respectively, and recurrence rate is the 1.64%th, the 12.8%th, 11.9% respectively；
2 three groups of different pharmaceutical result for the treatment of comparative test result of table
4 conclusions
Result of the test shows, the cure rate of medicine group of the present invention, drugs compared A group and drugs compared B group, efficient, multiple
Send out rate significant difference (P < 0.05).In terms of cure rate, medicine group of the present invention is not higher than drugs compared A group, drugs compared B component
27 percentage points and 24 percentage points；At efficient aspect, medicine group of the present invention is more other than drugs compared A group, drugs compared B component
High 23.7 percentage points and 12.3 percentage points, this fully proves that the result for the treatment of of medicine of the present invention is better than drugs compared A and right
Ratio medicine B.Illustrating that the compatibility between medicine taste of traditional Chinese medicine of the present invention is superior, the combination between flavour of a drug creates obvious Synergistic
Effect.Additionally, finding in this test that the mammitis of 33.3% suffers from ox is subclinical infection, does not shows obvious clinical inflammatory pathology, build
View plant pays much attention to the harm of recessive mastitis.
Experiment two: the quality determining method research of medicine of the present invention
1 instrument and reagent
1.1 instrument
(G1314 is purple for Agilent110 high performance liquid chromatograph and work station, G1311Aquat pump for high performance liquid chromatograph
External detector).
1.2 reagent
Salicin (salicin) reference substance (pharmaceutical biological product calibrating research institute of China)；Chinese medicine composition of the present invention；In
Medicinal material (offer of Kang Ji chain pharmacy)；Methyl alcohol (chromatogram alcohol, the biochemical work auxiliary reagent factory in Shanghai)；Other reagent is pure for analyzing.
2 methods and result
2.1 prescription
Purple Pueraria lobota 500g, Panicum repens 500g, purslane 250g.
2.2 preparation
Take dry purple Pueraria lobota 500g, Panicum repens 500g, purslane 250g, add 11000mL water soaking 1 hour for the first time, decoct
Boiling 1 hour, second time adds 4000mL, decocts 1 hour, merges decocting liquid, filters, and filtrate concentrates, and is dried, to obtain final product.
The assay of 2.3 salicins
2.3.1 HPLC chromatogram condition
Employing HypersilDs(4.0mm × 125mm, 5 μm) chromatographic column；Flowing phase: ratio is the methyl alcohol-0.15% of 30:70
Phosphoric acid solution；Detection wavelength: 200nm；Column temperature: 20 DEG C；Flow velocity: 1.0mL min-1；Sample size: 10 μ L；At this chromatographic condition
Under, reference substance and sample chromatogram peak are good, noiseless to measuring without Panicum repens negative control.
2.3.2 the preparation of reference substance solution
It is appropriate to the salicin comparison product of constant weight that precision weighs 80 DEG C of dryings, adds methyl alcohol and makes molten containing 0.2mg of every 1mL
Liquid.
2.3.3 the preparation of need testing solution and negative controls
Precision weighs medicine 10g of the present invention, adds methyl alcohol 40mL, is heated to reflux 4h, and extract reflux solvent is simultaneously concentrated to dryness,
Residue add water 10mL dissolve, with water saturated n-butanol shaking extract 5 times, each 20mL, merging n-butanol extracting liquid, try with ammonia
Liquid washs 3 times, each 15mL, and n-butanol extracting liquid recycling design is to doing, and residue adds methyl alcohol and dissolves and be transferred to 10mL volumetric flask
In, add methyl alcohol to scale, shake up, filter, take filtrate and get final product；The negative controls of Panicum repens is not separately contained in the preparation of prescription ratio,
It is made in the same way of negative controls.
2.3.4 the drafting of calibration curve
It is appropriate to the salicin comparison product of constant weight that precision weighs 80 DEG C of dryings, makes 10.4 with methyl alcohol, and 20.8,41.6,
83.2,166.4 μ gmL-1The solution of series concentration, precision measures each 10 μ L of above-mentioned 5 kinds of strength solution respectively, injects efficient liquid phase
Chromatograph is measured.
Carrying out linear regression with peak area ratio and concentration, obtaining regression equation is: A=21.2763C-0.1391, r=
0.9999.Show salicin at 10.4～166.4 μ gmL-1In good linear relationship in concentration range.
2.3.5 stability test
Accurate absorption need testing solution 10 μ L, respectively at 0,1,2,4,8h sample introduction, and calculate salicin content.In result 8h
RSD is 0.45%(n=5).Show that sample solution is stable in 8h.
2.3.6 replica test
By 5 parts of need testing solution preparation method parallel processing sample, measure salicin content in accordance with the law and calculate.Result records
Salicin average content is 0.12mg g-1, RSD is 1.3%.
2.3.7 Precision Experiment
Accurate extract water benzasalicin reference substance solution, repeats sample introduction 5 times, measures peak area in accordance with the law.Result RSD is 0.23%(n=
5).Show that precision is preferable.
2.3.8 rate of recovery experiment
Precision weighs 6 parts of the sample of the same lot number of known water benzasalicin content, adds by high, medium and low concentration precision respectively
Appropriate salicin comparison product solution, by operation under sample determination item, measures in accordance with the law, calculates the rate of recovery.Result average recovery rate
Being 100.3%, RSD is 0.45%(n=5).
2.3.9 sample size measures
Measuring reference substance solution respectively and need testing solution being appropriate, filter with miillpore filter, each sample introduction 10 μ L, by above-mentioned look
Spectral condition measures 3 batch samples, parallel determination 5 times.By external standard method with the content of calculated by peak area need testing solution salicin.This product
Aqueous benzasalicin should be and indicates the 95%～105% of content, in terms of the aqueous benzasalicin of every 1g sample, must not be less than 0.12mg.3 batch samples contain
Amount is respectively 100.8%(RSD=1.2%), 101.7%(RSD=1.3%), 99.2%(RSD=1.1%).
Detailed description of the invention:
Embodiment 1, a kind of medicine for treating mastitis for milk cows, this medicine is made up of the raw material of following weight portion
: purple Pueraria lobota 1 weight portion, Panicum repens 2 weight portion；Purslane 0.5 weight portion.
Embodiment 2, a kind of medicine for treating mastitis for milk cows, this medicine is made up of the raw material of following weight portion
: purple Pueraria lobota 2 weight portion, Panicum repens 1 weight portion；Purslane 1 weight portion.
Embodiment 3, a kind of medicine for treating mastitis for milk cows, this medicine is made up of the raw material of following weight portion
: purple Pueraria lobota 1 weight portion, Panicum repens 1 weight portion；Purslane 0.8 weight portion.
Embodiment 4, a kind of medicine for treating mastitis for milk cows, this medicine is made up of the raw material of following weight portion
: purple Pueraria lobota 1.5 weight portion, Panicum repens 1 weight portion;Purslane 0.7 weight portion.
Medicine described in any one in embodiment 1～4, this medicine preparation method routinely can be prepared as tablet, pill,
Powder, hard capsule, soft capsule, granule.
Embodiment 5, the medicine described in any one in embodiment 1～4, this medicine adopts and prepares with the following method: take purple Pueraria lobota,
Panicum repens, purslane, mixing, the water soaking of addition 3～15 times amount 0.5～2 hour, decoct 0.5～2 hour, decoct 2～4 times,
0.5～2 hour every time, extract merged, and filtered, and filtrate concentrates, and dry, pulverize into fine powder, added auxiliary material, mixes, makes scattered
Agent, to obtain final product.
Embodiment 6, the medicine described in any one in embodiment 1～4, this medicine is adopted and is prepared with the following method: take drying
Purple Pueraria lobota, Panicum repens, purslane, for the first time add 11 times amount water soaking 1 hour, decoct 1 hour, add 4 times amount soak by water 1 for the second time
Hour, merging decocting liquid, filter, filtrate concentrates, and dry, pulverize into fine powder, sieves, and mixes, makes powder, to obtain final product.
Embodiment 7, the medicine described in any one in embodiment 5 or 6, this medicine uses high performance liquid chromatography to carry out water
The assay of benzasalicin:
(1) chromatographic condition: use HypersilDs chromatographic column；Flowing phase: ratio be 20～40:60～80 methyl alcohol-
0.15% phosphoric acid solution；Detection wavelength: 190～210nm；Column temperature: 15～25 DEG C；Flow velocity: 0.5～1.5mL min-1；Sample size:
5～20 μ L；
(2) prepared by reference substance solution: it is appropriate that precision weighs the salicin comparison product being dried to constant weight, adds methyl alcohol dissolving and makes
Reference substance solution；
(3) preparation of need testing solution: precision weighs medicine of the present invention, adds methyl alcohol, is heated to reflux, extract reflux solvent
And be concentrated to dryness, residue is dissolved in water, and extracts with the shaking of water saturated n-butanol, merges n-butanol extracting liquid, washed by ammonia solution
Washing, n-butanol extracting liquid recycling design is to doing, and residue adds methyl alcohol and dissolves, and shakes up, and filters, takes filtrate, obtain need testing solution；
(4) measure: precision measures above-mentioned need testing solution, each 5～20 μ L of reference substance solution respectively, inject high-efficient liquid phase color
Spectrometer, detects.
Embodiment 8, the medicine described in any one in embodiment 5 or 6, use high performance liquid chromatography to carry out salicin
Assay:
(1) chromatographic condition: use HypersilDs chromatographic column；Flowing phase: ratio is that methyl alcohol-0.15% phosphoric acid of 30:70 is molten
Liquid；Detection wavelength: 200nm；Column temperature: 20 DEG C；Flow velocity: 1.0mL min-1；Sample size: 10 μ L；
(2) prepared by reference substance solution: it is appropriate to the salicin comparison product of constant weight that precision weighs 80 DEG C of dryings, adds methyl alcohol and dissolves
Make the reference substance solution containing 0.2mg for every 1mL；
(3) preparation of need testing solution: precision weighs medicine 10g of the present invention, adds methyl alcohol 40mL, is heated to reflux 4h, extracts
Liquid reflux solvent is simultaneously concentrated to dryness, residue add water 10mL dissolve, with water saturated n-butanol shaking extract 5 times, each 20mL, conjunction
And n-butanol extracting liquid, washing 3 times with ammonia solution, each 15mL, n-butanol extracting liquid recycling design is to doing, and it is molten that residue adds methyl alcohol
Solve and be transferred in 10mL volumetric flask, adding methyl alcohol to scale, shake up, filtering, take filtrate, obtain need testing solution；
(4) measure: precision measures above-mentioned need testing solution, each 10 μ L of reference substance solution respectively, inject high performance liquid chromatography
Instrument, detects.
Embodiment 9: pharmaceutical hard capsule agent of the present invention
Drug prescription: purple Pueraria lobota 500g, Panicum repens 500g, purslane 250g;
Preparation method: take dry purple Pueraria lobota 500g, Panicum repens 500g, purslane 250g;
Adding 11000mL water soaking 1 hour for the first time, decocting 1 hour, second time adds 4000mL, decocts 1 hour, merges water
Decocting liquid, filters, and filtrate concentrates, and dry, pulverize into fine powder, adds auxiliary material, mixes, makes powder, make 1000g.
High performance liquid chromatography is used to carry out assay to salicin (salicin):
(1) chromatographic condition: use HypersilDs chromatographic column；Flowing phase: ratio is that methyl alcohol-0.15% phosphoric acid of 30:70 is molten
Liquid；Detection wavelength: 200nm；Column temperature: 20 DEG C；Flow velocity: 1.0mL min-1；Sample size: 10 μ L；
(2) prepared by reference substance solution: it is appropriate to the salicin comparison product of constant weight that precision weighs 80 DEG C of dryings, adds methyl alcohol and makes
The solution containing 0.2mg for every 1mL；
(3) preparation of need testing solution: precision weighs medicine 10g of the present invention, adds methyl alcohol 40mL, is heated to reflux 4h, extracts
Liquid reflux solvent is simultaneously concentrated to dryness, residue add water 10mL dissolve, with water saturated n-butanol shaking extract 5 times, each 20mL, conjunction
And n-butanol extracting liquid, washing 3 times with ammonia solution, each 15mL, n-butanol extracting liquid recycling design is to doing, and it is molten that residue adds methyl alcohol
Solve and be transferred in 10mL volumetric flask, adding methyl alcohol to scale, shake up, filtering, take filtrate, obtain need testing solution；
(4) measure: precision measures above-mentioned need testing solution, each 10 μ L of reference substance solution respectively, inject high performance liquid chromatography
Instrument, detects, testing result be the content of salicin be 0.1712mg/g.
Although moreover, it will be appreciated that this specification is been described by according to embodiment, but not each embodiment only wraps
Containing an independent technical scheme, this narrating mode of specification is only that for clarity sake those skilled in the art should
Using specification as an entirety, the technical scheme in each embodiment also can form those skilled in the art through appropriately combined
May be appreciated other embodiments.
Claims (2)
1. the medicine being used for treating mastitis for milk cows, it is characterised in that this medicine is made up of the raw material of following weight portion
: purple Pueraria lobota 50g, Panicum repens 50g, purslane 25g；
This medicine is adopted and is prepared with the following method: take dry purple Pueraria lobota, Panicum repens, purslane, adds 11 times amount water soakings 1 for the first time little
When, decocting 1 hour, second time adds 4 times amount soak by water 1 hour, merges decocting liquid, filters, and filtrate concentrates, and dry, pulverize into thin
Powder, sieves, and mixes, makes powder, to obtain final product；
This medicine is adopted and is detected with the following method: use high performance liquid chromatography to carry out the assay of salicin:
(1) chromatographic condition: use HypersilDs chromatographic column；Flowing phase: ratio is methyl alcohol-0.15% phosphoric acid solution of 30:70；
Detection wavelength: 200nm；Column temperature: 20 DEG C；Flow velocity: 1.0mL min-1；Sample size: 10 μ L；
(2) prepared by reference substance solution: it is appropriate to the salicin comparison product of constant weight that precision weighs 80 DEG C of dryings, adds methyl alcohol dissolving and makes
The reference substance solution containing 0.2mg for every 1mL；
(3) preparation of need testing solution: precision weighs medicine 10g of the present invention, adds methyl alcohol 40mL, is heated to reflux 4h, and extract returns
Stream solvent is simultaneously concentrated to dryness, and the residue 10mL that adds water dissolves, and extracts 5 times with the shaking of water saturated n-butanol, each 20mL, and merging is just
Butanol extract, is washed 3 times by ammonia solution, each 15mL, and n-butanol extracting liquid recycling design is to doing, and residue adds methyl alcohol and dissolves simultaneously
It is transferred in 10mL volumetric flask, adds methyl alcohol to scale, shake up, filter, take filtrate, obtain need testing solution；
(4) measure: precision measures above-mentioned need testing solution, each 10 μ L of reference substance solution respectively, inject high performance liquid chromatograph, enter
Row detection.
2. application in the medicine or health products of preparation treatment ox chordapsus for the medicine as claimed in claim 1.
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| CN104189251A (en) * | 2014-08-19 | 2014-12-10 | 大连圣弘医药有限公司 | Traditional Chinese medicine composition for treating hyperplasia of mammary glands and benign breast tumors and preparation method of traditional Chinese medicine composition |
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| CN104189251A (en) * | 2014-08-19 | 2014-12-10 | 大连圣弘医药有限公司 | Traditional Chinese medicine composition for treating hyperplasia of mammary glands and benign breast tumors and preparation method of traditional Chinese medicine composition |
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| 小叶蛇葡萄根化学成分的研究;江贞仪;《第二军医大学学报》;19890630;第10卷(第6期);535 * |
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| 饲用药用植物的开发利用;翁长江;《中国草食动物》;19920331(第3期);29,36 * |
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