CN105168612A - Drug for treating piglet diarrhea, as well as preparation method, detection method and application thereof - Google Patents
Drug for treating piglet diarrhea, as well as preparation method, detection method and application thereof Download PDFInfo
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Abstract
The invention relates to the field of traditional Chinese medicines, particularly to a drug for treating piglet diarrhea, as well as a preparation method, a detection method and the application thereof. The drug is prepared from the following raw materials in parts by weight: 1-2 parts of sabina chinensis (L.) Ant.[Juiperus chinensis.L.] and 1-2 parts of caryopteris incana (Thunb.) Miq. [Nepeta incana Thunb. ex Houtt.]. The drug can be prepared into powders and granules. The preparation method comprises the following steps: soaking dry sabina chinensis (L.) Ant.[Juiperus chinensis.L.] and dried caryopteris incana (Thunb.) Miq. [Nepeta incana Thunb. ex Houtt.] with 11 times of water for 1 hour for the first time, decocting for 1 hour, decocting for 1 hour with 4 times of water for the second time, merging water decoctions, filtering, concentrating filtrate, drying, grinding into fine powders, screening, uniformly mixing, and preparing into powders. The chromatographic conditions in a drug quality detection method are that a HypersilDs chromatographic column is adopted; acetonitrile and a 0.05 mol/L sodium dihydrogen phosphate solution are adopted as moving phases, and the ratio of acetonitrile to the sodium dihydrogen phosphate solution is 30:70; the detection wavelength is 200 nm; the column temperature is 20 DEG C; the flow rate is 1.0 mL.min<-1>; and the sample amount is 10 [mu]L.
Description
Technical field
The present invention relates to the field of Chinese medicines, be specifically related to active medicine of a kind of Herba Caryopteridis Incanae and Folium et cacumen sabinae chinensis and preparation method thereof.
Background technology
Piglet diarrhea long-standing problem the development of pig industry, is one of key factor of restriction pig industry development.This cause of disease is popular wide, and falls ill throughout the year, and in Swine Production process, piglet diarrhea rate of dying of illness is high, and resistance to cross Piglet Development slow, and efficiency of feed utilization is low, causes heavy economic losses to breeding enterprise.At present in treatment piglet diarrhea medicament selection, the most frequently used still uses antibiotic or synthetic antimicrobial preparation, because these medicine life-time service easily cause the generation of Resistant strain, be unfavorable for the control of piglet diarrhea disease, therefore, Chinese herbal medicine additive is selected to get more and more to the research preventing and treating piglet diarrhea.
Summary of the invention
The object of the invention is for the deficiencies in the prior art, a kind of medicine for the treatment of piglet diarrhea is provided.
Another object of the present invention is to provide the preparation method of this medicine.
Present invention also offers the quality determining method of this medicine.
Present invention also offers the pharmaceutical applications of this medicine.
Medicine of the present invention meticulously develops for many years through inventor, is mainly used in treating piglet diarrhea.Medicine of the present invention can heat-clearing and toxic substances removing, promoting blood circulation to remove blood stasis, blood circulation of breast can be promoted again to dredge smooth, is conducive to scorching swollen absorption and dissipates, promote galactopoiesis, have good therapeutic effect to piglet diarrhea.
As follows at the Ji Yuan of medicine Raw medicine of the present invention:
Folium et cacumen sabinae chinensis is Cupressaceae Sabina Mill. plant Chinese juniper
sabinachinensis (L.) Ant. [JuiperuschinensisL.]leaf.
Herba Caryopteridis Incanae is Verenaceae Caryopteris plants Herba Caryopteridis Incanae
caryopterisincana (Thunb.) Miq. [NepetaincanaThunb.exHoutt.]herb.
The object of the invention is by realize with under type:
Medicine is made up of the raw material of following weight portion: Folium et cacumen sabinae chinensis 1 ~ 2 weight portion, Herba Caryopteridis Incanae 1 ~ 2 weight portion.
This medicine is preferably made up of the raw material of following weight portion: Folium et cacumen sabinae chinensis 1 weight portion, Herba Caryopteridis Incanae 2 weight portion.
This medicine is preferably made up of the raw material of following weight portion: Folium et cacumen sabinae chinensis 2 weight portion, Herba Caryopteridis Incanae 1 weight portion.
This medicine is preferably made up of the raw material of following weight portion: Folium et cacumen sabinae chinensis 1 weight portion, Herba Caryopteridis Incanae 1 weight portion.
Powder, granule can be prepared in described medicine.
Described medicine is adopted and is prepared with the following method: get Folium et cacumen sabinae chinensis, Herba Caryopteridis Incanae, and mixing adds the water soaking 0.5 ~ 2 hour of 3 ~ 15 times amount, decoct 0.5 ~ 2 hour, decoct 2 ~ 4 times, each 0.5 ~ 2 hour, extracting solution merges, and filters, and filtrate concentrates, dry, be ground into fine powder, add adjuvant, mixing, make powder, to obtain final product.
Described medicine is adopted and is prepared with the following method: get dry Folium et cacumen sabinae chinensis, Herba Caryopteridis Incanae, and first time adds 11 times amount water soaking 1 hour, decocts 1 hour, and second time adds 4 times amount soak by water 1 hour, merges decocting liquid, filter, filtrate concentrates, and dry, pulverize into fine powder, sieve, mixing, makes powder, to obtain final product.
Described medicine is adopted and is detected with the following method: adopt high performance liquid chromatography to carry out the assay of vanillic acid:
(1) chromatographic condition: adopt HypersilDs chromatographic column; Mobile phase: ratio is the acetonitrile-0.05mol/L sodium dihydrogen phosphate of 20 ~ 40:60 ~ 80; Determined wavelength: 190 ~ 210nm; Column temperature: 15 ~ 25 DEG C; Flow velocity: 0.5 ~ 1.5mLmin
-1; Sample size: 5 ~ 20 μ L;
(2) reference substance solution preparation: it is appropriate that precision takes the vanillic acid reference substance being dried to constant weight, adds dissolve with methanol and make reference substance solution;
(3) preparation of need testing solution: precision takes medicine of the present invention, adds methanol, reflux, extracting solution reflux solvent is also concentrated into dry, and residue is dissolved in water, and extracts with water saturated n-butyl alcohol jolting, merge n-butanol extracting liquid, with ammonia solution washing, n-butanol extracting liquid recycling design is to dry, and residue adds dissolve with methanol, shake up, filter, get filtrate, obtain need testing solution;
(4) measure: precision measures above-mentioned need testing solution, each 5 ~ 20 μ L of reference substance solution respectively, inject high performance liquid chromatograph, detect.
Described medicine is preferably adopted and is detected with the following method: adopt high performance liquid chromatography to carry out the assay of vanillic acid:
(1) chromatographic condition: adopt HypersilDs chromatographic column; Mobile phase: ratio is the acetonitrile-0.05mol/L sodium dihydrogen phosphate of 30:70; Determined wavelength: 200nm; Column temperature: 20 DEG C; Flow velocity: 1.0mLmin
-1; Sample size: 10 μ L;
(2) reference substance solution preparation: it is appropriate that precision takes 80 DEG C of vanillic acid reference substances being dried to constant weight, adds dissolve with methanol and makes the reference substance solution of every 1mL containing 0.2mg;
(3) preparation of need testing solution: precision takes medicine 10g of the present invention, adds methanol 40mL, reflux 4h, extracting solution reflux solvent is also concentrated into dry, residue add water 10mL dissolve, extract 5 times with water saturated n-butyl alcohol jolting, each 20mL, merges n-butanol extracting liquid, washs 3 times with ammonia solution, each 15mL, n-butanol extracting liquid recycling design is to dry, and residue adds dissolve with methanol and is transferred in 10mL volumetric flask, add methanol to scale, shake up, filter, get filtrate, obtain need testing solution;
(4) measure: precision measures above-mentioned need testing solution, each 10 μ L of reference substance solution respectively, inject high performance liquid chromatograph, detect.
Described medicine can be used for the medicine preparing treatment piglet diarrhea, pig blue-ear disease.
experiment one: the experimental study of Drug therapy piglet diarrhea effect of the present invention
1 test material
1.1 test piglets are selected and grouping
The date close piglet 60 selecting the close healthy sow of age parity to produce, be divided into 4 groups, be respectively group 1, group 2, group 3, group 4, often organize 15, immune disinfectant program is undertaken by pig farm conventional method, under the horizontal the same terms of feeding and management, to clinical manifestation diarrhoea before and after wean, loose and watery stool is soft, and defecation frequency increases, the listless pigling that diarrhoea occurs carries out gavaging administration, carries out contrast test respectively to matched group injection mequindox injection and ciprofloxacin injection simultaneously.
1.2 test medicine and grouping
Medicine of the present invention: prescription: Folium et cacumen sabinae chinensis 50g, Herba Caryopteridis Incanae 50g; Preparation method: get dry Folium et cacumen sabinae chinensis 50g, Herba Caryopteridis Incanae 50g and add 1100mL water soaking 1 hour for the first time, decoct 1 hour, second time adds 400mL, decocts 1 hour, merges decocting liquid, filters, and filtrate concentrates, and drying, obtains medicine of the present invention.
Drugs compared A: prescription: Folium et cacumen sabinae chinensis 100g; Preparation method: get dry Folium et cacumen sabinae chinensis 100g, first time adds 1100mL water soaking 1 hour, decocts 1 hour, and second time adds 400mL, decocts 1 hour, merges decocting liquid, filters, and filtrate concentrates, dry, obtains drugs compared A.
Drugs compared B: prescription: Herba Caryopteridis Incanae 100g; Preparation method: get dry Herba Caryopteridis Incanae 100g, first time adds 1100mL water soaking 1 hour, decocts 1 hour, and second time adds 400mL, decocts 1 hour, merges decocting liquid, filters, and filtrate concentrates, dry, obtains drugs compared B.
Group 1 gives medicine of the present invention, and group 2 gives drugs compared A, and group 3 gives drugs compared B, group 4 injection ciprofloxacin injection.
2 test methods
1st group with medicine of the present invention to piglet administered by oral gavage administration 5g, 1d1 time; 2nd group with drugs compared A to piglet administered by oral gavage administration 5g, 1d1 time; 3rd group with drugs compared B to piglet administered by oral gavage administration 5g, 1d1 time; 4th group with ciprofloxacin injection drug administration by injection 1mg/kg.Equal continuous use 5d, observes pigling diarrhoea situation of change every day.
Medication effect observation judges: become normally to observation post administration piglet appetite, feces situation, be decided to be healing; The piglet mental status takes a turn for the better gradually, and pellet morphology improvement is decided to be effectively, and effective percentage calculates according to healing number and remission number sum; Feces is still for water sample and hemafecia are decided to be invalid, and inefficiency is according to death toll and invalid number sum and total ratio calculation.
3 result of the tests
From table 1 result of the test, after piglet medication, in cure rate, medicine of the present invention 1 group is the highest, reaches 86.7%, and all the other are followed successively by the 4th group 73.3%, the 2nd group the 67.7%, 3rd group 60%.
In effective percentage, the highest is 1 group 86.7%, all the other are the 4th group 73.3%, the 2nd group the 67.7%, 3rd group 60%.
In inefficiency, the highest is 3 groups 40%, be secondly the 2nd group 33.3%, the 4th group 26.7%, minimum is 1 group 13.3%, have obvious difference.
This tests explanation, and medicine of the present invention is Be very effective in the pigling diarrhoea for the treatment of wean front and back.
Table 1 different pharmaceutical is to piglet diarrhea therapeutic effect statistical table unit: number (head), rate (%)
4 results and discussion
Result of the test shows, four kinds of medicines of test group all have the effect of obviously treatment piglet diarrhea, and wherein the highest with the cure rate of piglet medication test group 1, effective percentage, reach 86.7%, exceed matched group 3 groups and reach 26.7%, effective percentage exceeds matched group 2 group 19%; Mortality rate aspect, 1 group minimum 6.7%, 2 groups and 3 groups are 20%, and 4 groups is 13.3%; What inefficiency was the highest is 3 groups 40%, be secondly 26.7% of 2 group 33.3% and 4 groups, minimum is 1 group 13.3%, also show that the therapeutic effect of 1 group is also better than other each group.The therapeutic effect of this sufficient proof medicine of the present invention is better than drugs compared A and drugs compared B.Illustrate that the compatibility in medicine of the present invention between two kinds of flavour of a drug is superior, indispensable, the combination between two kinds of flavour of a drug creates obvious synergistic function, creates the technique effect that one-plus-one is greater than two.In test, the therapeutic effect of 4 groups of ciprofloxacin injections is poor, may with raising, and medicine life-time service, producing drug resistance has certain relation.
experiment two: the quality determining method research of medicine of the present invention
1 instrument and reagent
1.1 instrument
High performance liquid chromatograph (Agilent110 high performance liquid chromatograph and work station, G1311Aquat pump, G1314 UV-detector).
1.2 reagent
Vanillic acid (vanillicacid) reference substance (Chinese pharmaceutical biological product calibrating academy); Chinese medicine composition of the present invention; Chinese crude drug (Kang Ji chain pharmacy provides); Methanol (chromatograph alcohol, biochemical work auxiliary reagent factory, Shanghai); Other reagent are analytical pure.
2 methods and result
2.1 prescription
Folium et cacumen sabinae chinensis 500g, Herba Caryopteridis Incanae 500g.
2.2 preparation
Get dry Folium et cacumen sabinae chinensis 500g, Herba Caryopteridis Incanae 500g, first time adds 11000mL water soaking 1 hour, decocts 1 hour, and second time adds 4000mL, decocts 1 hour, merges decocting liquid, filters, and filtrate concentrates, dry, to obtain final product.
The assay of 2.3 vanillic acids (vanillicacid)
2.3.1HPLC chromatographic condition
Adopt HypersilDs(4.0mm × 125mm, 5 μm) chromatographic column; Mobile phase: ratio is the acetonitrile-0.05mol/L sodium dihydrogen phosphate of 30:70; Determined wavelength: 200nm; Column temperature: 20 DEG C; Flow velocity: 1.0mLmin
-1; Sample size: 10 μ L; Under this chromatographic condition, reference substance and sample chromatogram peak are well, noiseless to mensuration without Herba Caryopteridis Incanae negative control.
2.3.2 the preparation of reference substance solution
It is appropriate that precision takes 80 DEG C of vanillic acid reference substances being dried to constant weight, adds methanol and make the solution of every 1mL containing 0.2mg.
2.3.3 the preparation of need testing solution and negative controls
Precision takes medicine 10g of the present invention, adds methanol 40mL, reflux 4h, extracting solution reflux solvent is also concentrated into dry, residue add water 10mL dissolve, extract 5 times with water saturated n-butyl alcohol jolting, each 20mL, merges n-butanol extracting liquid, washs 3 times with ammonia solution, each 15mL, n-butanol extracting liquid recycling design is to dry, and residue adds dissolve with methanol and is transferred in 10mL volumetric flask, add methanol to scale, shake up, filter, get filtrate and get final product; Separately do not contain the negative controls of Herba Caryopteridis Incanae in the preparation of prescription ratio, be made in the same way of negative controls.
2.3.4 the drafting of standard curve
It is appropriate that precision takes 80 DEG C of vanillic acid reference substances being dried to constant weight, makes 10.4,20.8,41.6,83.2,166.4 μ gmL with methanol
-1the solution of series concentration, precision measures each 10 μ L of above-mentioned 5 kinds of strength solution respectively, injects high performance liquid chromatograph and measures.
Carry out linear regression with peak area ratio and concentration, obtaining regression equation is: A=21.2763C-0.1391, r=0.9999.Show that vanillic acid is at 10.4 ~ 166.4 μ gmL
-1in good linear relationship in concentration range.
2.3.5 stability test
Accurate absorption need testing solution 10 μ L, respectively at 0,1,2,4,8h sample introduction, and calculates vanillic acid content.In result 8h, RSD is 0.45%(n=5).Show that sample solution is stable in 8h.
2.3.6 replica test
By 5 parts, need testing solution preparation method parallel processing sample, measure vanillic acid content in accordance with the law and calculate.It is 0.13mgg that result records vanillic acid average content
-1, RSD is 1.3%.
2.3.7 Precision Experiment
Accurate absorption vanillic acid reference substance solution, repeats sample introduction 5 times, measures peak area in accordance with the law.Result RSD is 0.22%(n=5).Show that precision is better.
2.3.8 response rate experiment
Precision takes 6 parts, the sample of the same lot number of known vanillic acid content, adds appropriate vanillic acid reference substance solution, operate, measure in accordance with the law, calculate the response rate by under sample determination item by high, medium and low concentration is accurate respectively.Result average recovery rate is 100.2%, RSD is 0.44%(n=5).
2.3.9 sample size measures
Measure reference substance solution and need testing solution respectively appropriate, filter with microporous filter membrane, each sample introduction 10 μ L, measures 3 batch samples by above-mentioned chromatographic condition, parallel assay 5 times.By external standard method with the content of calculated by peak area need testing solution vanillic acid.This product should be containing vanillic acid and indicates 96% ~ 104% of content, in every 1g sample containing vanillic acid, must not be less than 0.13mg.3 batch sample content are respectively 100.1%(RSD=1.1%), 101.2%(RSD=1.2%), 99.8%(RSD=1.0%).
detailed description of the invention:
Embodiment 1, a kind of medicine for the treatment of piglet diarrhea, this medicine is made up of the raw material of following weight portion: Folium et cacumen sabinae chinensis 1 weight portion, Herba Caryopteridis Incanae 2 weight portion.
Embodiment 2, a kind of medicine for the treatment of piglet diarrhea, this medicine is made up of the raw material of following weight portion: Folium et cacumen sabinae chinensis 2 weight portion, Herba Caryopteridis Incanae 1 weight portion.
Embodiment 3, a kind of medicine for the treatment of piglet diarrhea, this medicine is made up of the raw material of following weight portion: Folium et cacumen sabinae chinensis 1 weight portion, Herba Caryopteridis Incanae 1 weight portion.
Embodiment 4, a kind of medicine for the treatment of piglet diarrhea, this medicine is made up of the raw material of following weight portion: Folium et cacumen sabinae chinensis 1.5 weight portion, Herba Caryopteridis Incanae 1 weight portion.
Embodiment 5, the medicine in embodiment 1 ~ 4 described in any one, this medicine can be prepared into powder, granule by art methods.
Embodiment 6, the medicine in embodiment 1 ~ 4 described in any one, this medicine is adopted and is prepared with the following method: get Folium et cacumen sabinae chinensis, Herba Caryopteridis Incanae, mixing, adds the water soaking 0.5 ~ 2 hour of 3 ~ 15 times amount, decocts 0.5 ~ 2 hour, decoct 2 ~ 4 times, each 0.5 ~ 2 hour, extracting solution merges, filter, filtrate concentrates, dry, be ground into fine powder, add adjuvant, mixing, make powder, to obtain final product.
Embodiment 7, the medicine in embodiment 1 ~ 4 described in any one, this medicine is adopted and is prepared with the following method: get dry Folium et cacumen sabinae chinensis, Herba Caryopteridis Incanae, first time adds 11 times amount water soaking 1 hour, decocts 1 hour, and second time adds 4 times amount soak by water 1 hour, merge decocting liquid, filter, filtrate concentrates, dry, be ground into fine powder, sieve, mixing, make powder, to obtain final product.
Embodiment 8, the medicine described in embodiment 6 or 7, this medicine is adopted and is detected with the following method: adopt high performance liquid chromatography to carry out the assay of vanillic acid:
(1) chromatographic condition: adopt HypersilDs chromatographic column; Mobile phase: ratio is the acetonitrile-0.05mol/L sodium dihydrogen phosphate of 20 ~ 40:60 ~ 80; Determined wavelength: 190 ~ 210nm; Column temperature: 15 ~ 25 DEG C; Flow velocity: 0.5 ~ 1.5mLmin
-1; Sample size: 5 ~ 20 μ L;
(2) reference substance solution preparation: it is appropriate that precision takes the vanillic acid reference substance being dried to constant weight, adds dissolve with methanol and make reference substance solution;
(3) preparation of need testing solution: precision takes medicine of the present invention, adds methanol, reflux, extracting solution reflux solvent is also concentrated into dry, and residue is dissolved in water, and extracts with water saturated n-butyl alcohol jolting, merge n-butanol extracting liquid, with ammonia solution washing, n-butanol extracting liquid recycling design is to dry, and residue adds dissolve with methanol, shake up, filter, get filtrate, obtain need testing solution;
(4) measure: precision measures above-mentioned need testing solution, each 5 ~ 20 μ L of reference substance solution respectively, inject high performance liquid chromatograph, detect.
Embodiment 9, the medicine described in embodiment 6 or 7, this medicine is adopted and is detected with the following method: adopt high performance liquid chromatography to carry out the assay of vanillic acid:
(1) chromatographic condition: adopt HypersilDs chromatographic column; Mobile phase: ratio is the acetonitrile-0.05mol/L sodium dihydrogen phosphate of 30:70; Determined wavelength: 200nm; Column temperature: 20 DEG C; Flow velocity: 1.0mLmin
-1; Sample size: 10 μ L;
(2) reference substance solution preparation: it is appropriate that precision takes 80 DEG C of vanillic acid reference substances being dried to constant weight, adds dissolve with methanol and makes the reference substance solution of every 1mL containing 0.2mg;
(3) preparation of need testing solution: precision takes medicine 10g of the present invention, adds methanol 40mL, reflux 4h, extracting solution reflux solvent is also concentrated into dry, residue add water 10mL dissolve, extract 5 times with water saturated n-butyl alcohol jolting, each 20mL, merges n-butanol extracting liquid, washs 3 times with ammonia solution, each 15mL, n-butanol extracting liquid recycling design is to dry, and residue adds dissolve with methanol and is transferred in 10mL volumetric flask, add methanol to scale, shake up, filter, get filtrate, obtain need testing solution;
(4) measure: precision measures above-mentioned need testing solution, each 10 μ L of reference substance solution respectively, inject high performance liquid chromatograph, detect.
embodiment 10:treat a medicine for piglet diarrhea,
Prescription: Folium et cacumen sabinae chinensis 50g, Herba Caryopteridis Incanae 50g
Preparation method: get dry Folium et cacumen sabinae chinensis 50g, Herba Caryopteridis Incanae 50g and add 1100mL water soaking 1 hour for the first time, decoct 1 hour, second time adds 400mL, decocts 1 hour, merges decocting liquid, filters, and filtrate concentrates, and drying, obtains medicine of the present invention.
embodiment 11:a kind of hard capsule for the treatment of piglet diarrhea
Drug prescription: Folium et cacumen sabinae chinensis 500g, Herba Caryopteridis Incanae 500g
Preparation method: get dry Folium et cacumen sabinae chinensis 500g, Herba Caryopteridis Incanae 500g, first time adds 11000mL water soaking 1 hour, decocts 1 hour, and second time adds 4000mL, decoct 1 hour, merge decocting liquid, filter, filtrate concentrates, dry, be ground into fine powder, add adjuvant, mixing, makes powder, makes 1000g.
High performance liquid chromatography is adopted to carry out assay to vanillic acid (vanillicacid):
(1) chromatographic condition: adopt HypersilDs chromatographic column; Mobile phase: ratio is the acetonitrile-0.05mol/L sodium dihydrogen phosphate of 30:70; Determined wavelength: 200nm; Column temperature: 20 DEG C; Flow velocity: 1.0mLmin
-1; Sample size: 10 μ L;
(2) reference substance solution preparation: it is appropriate that precision takes 80 DEG C of vanillic acid reference substances being dried to constant weight, adds methanol and makes the solution of every 1mL containing 0.2mg;
(3) preparation of need testing solution: precision takes medicine 10g of the present invention, adds methanol 40mL, reflux 4h, extracting solution reflux solvent is also concentrated into dry, residue add water 10mL dissolve, extract 5 times with water saturated n-butyl alcohol jolting, each 20mL, merges n-butanol extracting liquid, washs 3 times with ammonia solution, each 15mL, n-butanol extracting liquid recycling design is to dry, and residue adds dissolve with methanol and is transferred in 10mL volumetric flask, add methanol to scale, shake up, filter, get filtrate, obtain need testing solution;
(4) measure: precision measures above-mentioned need testing solution, each 10 μ L of reference substance solution respectively, inject high performance liquid chromatograph, detect, testing result is the content of vanillic acid is 0.1637mg/g.
In addition, be to be understood that, although this description is described according to embodiment, but not each embodiment only comprises an independently technical scheme, this narrating mode of description is only for clarity sake, those skilled in the art should by description integrally, and the technical scheme in each embodiment also through appropriately combined, can form other embodiments that it will be appreciated by those skilled in the art that.
Claims (10)
1. treat a medicine for piglet diarrhea, it is characterized in that, this medicine is made up of the raw material of following weight portion: Folium et cacumen sabinae chinensis 1 ~ 2 weight portion, Herba Caryopteridis Incanae 1 ~ 2 weight portion.
2. medicine as claimed in claim 1, it is characterized in that, this medicine is made up of the raw material of following weight portion: Folium et cacumen sabinae chinensis 1 weight portion, Herba Caryopteridis Incanae 2 weight portion.
3. medicine as claimed in claim 1, it is characterized in that, this medicine is made up of the raw material of following weight portion: Folium et cacumen sabinae chinensis 2 weight portion, Herba Caryopteridis Incanae 1 weight portion.
4. as claim 1 medicine, it is characterized in that, this medicine is made up of the raw material of following weight portion: Folium et cacumen sabinae chinensis 1 weight portion, Herba Caryopteridis Incanae 1 weight portion.
5. as the medicine in Claims 1 to 4 as described in any one, it is characterized in that, this medicine is prepared into powder, granule.
6. medicine as claimed in claim 5, is characterized in that, this medicine is adopted and prepared with the following method: get Folium et cacumen sabinae chinensis, Herba Caryopteridis Incanae, mixing, adds the water soaking 0.5 ~ 2 hour of 3 ~ 15 times amount, decocts 0.5 ~ 2 hour, decoct 2 ~ 4 times, each 0.5 ~ 2 hour, extracting solution merges, filter, filtrate concentrates, dry, be ground into fine powder, add adjuvant, mixing, make powder, to obtain final product.
7. medicine as claimed in claim 6, is characterized in that, this medicine is adopted and prepared with the following method: get dry Folium et cacumen sabinae chinensis, Herba Caryopteridis Incanae, first time adds 11 times amount water soaking 1 hour, decocts 1 hour, and second time adds 4 times amount soak by water 1 hour, merge decocting liquid, filter, filtrate concentrates, dry, be ground into fine powder, sieve, mixing, make powder, to obtain final product.
8. as the medicine in Claims 1 to 4 as described in any one, it is characterized in that, this medicine is adopted and is detected with the following method: adopt high performance liquid chromatography to carry out the assay of vanillic acid:
(1) chromatographic condition: adopt HypersilDs chromatographic column; Mobile phase: ratio is the acetonitrile-0.05mol/L sodium dihydrogen phosphate of 20 ~ 40:60 ~ 80; Determined wavelength: 190 ~ 210nm; Column temperature: 15 ~ 25 DEG C; Flow velocity: 0.5 ~ 1.5mLmin
-1; Sample size: 5 ~ 20 μ L;
(2) reference substance solution preparation: it is appropriate that precision takes the vanillic acid reference substance being dried to constant weight, adds dissolve with methanol and make reference substance solution;
(3) preparation of need testing solution: precision takes medicine of the present invention, adds methanol, reflux, extracting solution reflux solvent is also concentrated into dry, and residue is dissolved in water, and extracts with water saturated n-butyl alcohol jolting, merge n-butanol extracting liquid, with ammonia solution washing, n-butanol extracting liquid recycling design is to dry, and residue adds dissolve with methanol, shake up, filter, get filtrate, obtain need testing solution;
(4) measure: precision measures above-mentioned need testing solution, each 5 ~ 20 μ L of reference substance solution respectively, inject high performance liquid chromatograph, detect.
9. medicine as claimed in claim 8, is characterized in that, this medicine is adopted and detected with the following method: adopt high performance liquid chromatography to carry out the assay of vanillic acid:
(1) chromatographic condition: adopt HypersilDs chromatographic column; Mobile phase: ratio is the acetonitrile-0.05mol/L sodium dihydrogen phosphate of 30:70; Determined wavelength: 200nm; Column temperature: 20 DEG C; Flow velocity: 1.0mLmin
-1; Sample size: 10 μ L;
(2) reference substance solution preparation: it is appropriate that precision takes 80 DEG C of vanillic acid reference substances being dried to constant weight, adds dissolve with methanol and makes the reference substance solution of every 1mL containing 0.2mg;
(3) preparation of need testing solution: precision takes medicine 10g of the present invention, adds methanol 40mL, reflux 4h, extracting solution reflux solvent is also concentrated into dry, residue add water 10mL dissolve, extract 5 times with water saturated n-butyl alcohol jolting, each 20mL, merges n-butanol extracting liquid, washs 3 times with ammonia solution, each 15mL, n-butanol extracting liquid recycling design is to dry, and residue adds dissolve with methanol and is transferred in 10mL volumetric flask, add methanol to scale, shake up, filter, get filtrate, obtain need testing solution;
(4) measure: precision measures above-mentioned need testing solution, each 10 μ L of reference substance solution respectively, inject high performance liquid chromatograph, detect.
10. as the application of the medicine in Claims 1 to 4 as described in any one in the medicine of preparation treatment pig blue-ear disease.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110574839A (en) * | 2019-09-02 | 2019-12-17 | 湖南农业大学 | Weaned piglet feed |
| CN113956369A (en) * | 2021-10-21 | 2022-01-21 | 河南省医药科学研究院 | Preparation method and application of Caryopteris clandonensis polysaccharide |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103690841A (en) * | 2013-12-30 | 2014-04-02 | 山东滨州博莱威生物技术有限公司 | Traditional Chinese medicine for treating diarrhea of weaned piglet in summer and preparation method thereof |
| CN104622932A (en) * | 2015-02-06 | 2015-05-20 | 郭敏 | Traditional Chinese medicine for treating myocardial ischemia and preparation method, detection method and application thereof |
| CN104758471A (en) * | 2015-03-06 | 2015-07-08 | 钟云仙 | Chinese medicinal composition for treatment of piglet diarrhea |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN103690841A (en) * | 2013-12-30 | 2014-04-02 | 山东滨州博莱威生物技术有限公司 | Traditional Chinese medicine for treating diarrhea of weaned piglet in summer and preparation method thereof |
| CN104622932A (en) * | 2015-02-06 | 2015-05-20 | 郭敏 | Traditional Chinese medicine for treating myocardial ischemia and preparation method, detection method and application thereof |
| CN104758471A (en) * | 2015-03-06 | 2015-07-08 | 钟云仙 | Chinese medicinal composition for treatment of piglet diarrhea |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110574839A (en) * | 2019-09-02 | 2019-12-17 | 湖南农业大学 | Weaned piglet feed |
| CN113956369A (en) * | 2021-10-21 | 2022-01-21 | 河南省医药科学研究院 | Preparation method and application of Caryopteris clandonensis polysaccharide |
| CN113956369B (en) * | 2021-10-21 | 2022-09-27 | 河南省医药科学研究院 | Preparation method and application of Caryopteris clandonensis polysaccharide |
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