CN103977391A - Preparation method and applications of bupleurum tenue capsule - Google Patents
Preparation method and applications of bupleurum tenue capsule Download PDFInfo
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- 238000000194 supercritical-fluid extraction Methods 0.000 claims abstract description 23
- 206010025323 Lymphomas Diseases 0.000 claims abstract description 9
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- 238000000605 extraction Methods 0.000 claims description 17
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- 235000006886 Zingiber officinale Nutrition 0.000 abstract 2
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- 244000303040 Glycyrrhiza glabra Species 0.000 abstract 1
- 235000006200 Glycyrrhiza glabra Nutrition 0.000 abstract 1
- 241001522232 Pinellia ternata Species 0.000 abstract 1
- 240000004534 Scutellaria baicalensis Species 0.000 abstract 1
- 235000017089 Scutellaria baicalensis Nutrition 0.000 abstract 1
- 240000000038 Ziziphus mauritiana Species 0.000 abstract 1
- 235000006545 Ziziphus mauritiana Nutrition 0.000 abstract 1
- 235000008529 Ziziphus vulgaris Nutrition 0.000 abstract 1
- 239000004480 active ingredient Substances 0.000 abstract 1
- 239000008186 active pharmaceutical agent Substances 0.000 abstract 1
- 235000011389 fruit/vegetable juice Nutrition 0.000 abstract 1
- LPLVUJXQOOQHMX-QWBHMCJMSA-N glycyrrhizinic acid Chemical compound O([C@@H]1[C@@H](O)[C@H](O)[C@H](O[C@@H]1O[C@@H]1C([C@H]2[C@]([C@@H]3[C@@]([C@@]4(CC[C@@]5(C)CC[C@@](C)(C[C@H]5C4=CC3=O)C(O)=O)C)(C)CC2)(C)CC1)(C)C)C(O)=O)[C@@H]1O[C@H](C(O)=O)[C@@H](O)[C@H](O)[C@H]1O LPLVUJXQOOQHMX-QWBHMCJMSA-N 0.000 abstract 1
- 235000011477 liquorice Nutrition 0.000 abstract 1
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- 238000002474 experimental method Methods 0.000 description 8
- 239000000243 solution Substances 0.000 description 8
- 238000012360 testing method Methods 0.000 description 6
- 210000001519 tissue Anatomy 0.000 description 5
- IAZDPXIOMUYVGZ-UHFFFAOYSA-N Dimethylsulphoxide Chemical compound CS(C)=O IAZDPXIOMUYVGZ-UHFFFAOYSA-N 0.000 description 4
- 241000699670 Mus sp. Species 0.000 description 4
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical group [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 4
- 239000003153 chemical reaction reagent Substances 0.000 description 4
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- 239000006144 Dulbecco’s modified Eagle's medium Substances 0.000 description 3
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 3
- 229910019142 PO4 Inorganic materials 0.000 description 3
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- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 2
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 2
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- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- WEEMDRWIKYCTQM-UHFFFAOYSA-N 2,6-dimethoxybenzenecarbothioamide Chemical compound COC1=CC=CC(OC)=C1C(N)=S WEEMDRWIKYCTQM-UHFFFAOYSA-N 0.000 description 1
- CMSMOCZEIVJLDB-UHFFFAOYSA-N Cyclophosphamide Chemical compound ClCCN(CCCl)P1(=O)NCCCO1 CMSMOCZEIVJLDB-UHFFFAOYSA-N 0.000 description 1
- 238000002965 ELISA Methods 0.000 description 1
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- FCPVYOBCFFNJFS-LQDWTQKMSA-M benzylpenicillin sodium Chemical compound [Na+].N([C@H]1[C@H]2SC([C@@H](N2C1=O)C([O-])=O)(C)C)C(=O)CC1=CC=CC=C1 FCPVYOBCFFNJFS-LQDWTQKMSA-M 0.000 description 1
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- 238000006386 neutralization reaction Methods 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
- YTJSFYQNRXLOIC-UHFFFAOYSA-N octadecylsilane Chemical compound CCCCCCCCCCCCCCCCCC[SiH3] YTJSFYQNRXLOIC-UHFFFAOYSA-N 0.000 description 1
- 210000004279 orbit Anatomy 0.000 description 1
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- Medicines Containing Plant Substances (AREA)
- Medicinal Preparation (AREA)
Abstract
The invention provides a preparation method of a bupleurum tenue capsule. The bupleurum tenue capsule is prepared from the following active pharmaceutical ingredients: 445g of bupleurum tenue, 222g of pinellia ternata stir-baked with ginger juice, 167g of scutellaria baicalensis, 167g of codonopsis pilosula, 167g of liquorice, 167g of fresh ginger and 167g of Chinese dates. The bupleurum tenue capsule is prepared by means of supercritical extraction, so that the content of active ingredients is increased greatly and the dosage is reduced. The invention also provides the applications of the bupleurum tenue capsule in preparing medicines for inhibiting proliferation of human histocyte lymphoma U937 cells and medicines for increasing red blood cells.
Description
Technical field
The present invention relates to Chinese medicine preparation technical field, be specifically related to a kind of preparation method and application of little chaihu capsules.
Background technology
Little chaihu capsules is recorded in ministry standard, prescription is Radix Bupleuri 445g, processed with Rhizoma Zingiberis Recens Rhizoma Pinelliae 222g, Radix Scutellariae 167g, Radix Codonopsis 167g, Radix Glycyrrhizae 167g, Rhizoma Zingiberis Recens 167g, Fructus Jujubae 167g, above seven tastes, Radix Codonopsis 45g and Radix Glycyrrhizae powder are broken into fine powder, get 96g standby, and all the other fine powders and residue Radix Codonopsis and Radix Bupleuri, Radix Scutellariae, Fructus Jujubae decoct with water secondary, each 1.5 hours, merging filtrate, filters, and it is 1.0-1.2 (80 ℃) that filtrate is concentrated into relative density; Rhizoma Pinelliae (processed with Rhizoma Zingiberis Recens), Rhizoma Zingiberis Recens, according to the percolation (appendix IO of Chinese Pharmacopoeia version in 2010) under fluid extract and extractum item, are made solvent with 70% ethanol, flood after 24 hours, with the speed of 1-3ml per minute percolation slowly, collect the about 900ml of percolate, reclaim ethanol, merge with concentrated solution, being condensed into relative density is the thick paste of 1.20-1.25 (80 ℃), adds above-mentioned standby fine powder, mix, dry, porphyrize, granulation, dry, make 1000 capsules, obtain.
In prior art, not yet there is little chaihu capsules aspect extraction preparation, adopting the report of supercritical technology, and the method that adopts decocting to boil, technique is coarse, backward, and impurity is many, causes patient's consumption excessive, is inconvenient to take, and has had a strong impact on this product and has applied clinically.
Summary of the invention
Goal of the invention: in order to address the above problem, the object of the present invention is to provide a kind of preparation method of little chaihu capsules.
Another object of the present invention is to provide a kind of little chaihu capsules to suppress the application in the lymphoma U937 of human tissue cell cell proliferation medicine and rising erythrocyte medicine in preparation.
Technical scheme: the object of the invention is to realize by following scheme:
A kind of preparation method of little chaihu capsules, by Radix Bupleuri 445g, processed with Rhizoma Zingiberis Recens Rhizoma Pinelliae 222g, Radix Scutellariae 167g, Radix Codonopsis 167g, Radix Glycyrrhizae 167g, Rhizoma Zingiberis Recens 167g, Fructus Jujubae 167g, as crude drug, made, described method is comprised of the following step: Radix Bupleuri 445g, processed with Rhizoma Zingiberis Recens Rhizoma Pinelliae 222g, Radix Scutellariae 167g, Radix Codonopsis 167g, Radix Glycyrrhizae 167g, Rhizoma Zingiberis Recens 167g, Fructus Jujubae 167g join CO
2in supercritical extraction device, ethanol is as entrainer, and the percent by volume that entrainer accounts for total extractant is 4-6%, extracting pressure 15-30MPa, temperature 30-50 ℃, CO
2flow 1-3m1/g crude drug min, extraction time 150-180min, obtains supercritical extract, and the thick paste that while being condensed into 80 ℃, relative density is 1.20-1.25, adds starch, mixes, dry, porphyrize, granulation, dry, make 1000 capsules, obtain.
The preparation method of above-mentioned little chaihu capsules, described CO
2the percent by volume that supercritical extraction entrainer accounts for total extractant is 5%.
The preparation method of above-mentioned little chaihu capsules, described CO
2the extracting pressure 20MPa of supercritical extraction, 40 ℃ of temperature, CO
2flow 2ml/g crude drug min, extraction time 160min.
Above-mentioned little chaihu capsules suppresses the application in the lymphoma U937 of human tissue cell cell proliferation medicine in preparation, little chaihu capsules is by Radix Bupleuri 445g, processed with Rhizoma Zingiberis Recens Rhizoma Pinelliae 222g, Radix Scutellariae 167g, Radix Codonopsis 167g, Radix Glycyrrhizae 167g, Rhizoma Zingiberis Recens 167g, Fructus Jujubae 167g makes as crude drug, preparation method is comprised of the following step: get Radix Bupleuri 445g, processed with Rhizoma Zingiberis Recens Rhizoma Pinelliae 222g, Radix Scutellariae 167g, Radix Codonopsis 167g, Radix Glycyrrhizae 167g, Rhizoma Zingiberis Recens 167g, Fructus Jujubae 167g joins in CO2 supercritical extraction device, ethanol is as entrainer, the percent by volume that entrainer accounts for total extractant is 4-6%, extracting pressure 15-30MPa, temperature 30-50 ℃, CO2 flow 1-3m1/g crude drug min, extraction time 150-180min, obtain supercritical extract, the thick paste that while being condensed into 80 ℃, relative density is 1.20-1.25, add starch, mix, dry, porphyrize, granulation, dry, make 1000 capsules, obtain.
Above-mentioned little chaihu capsules suppresses the application in the lymphoma U937 of human tissue cell cell proliferation medicine, CO described in little chaihu capsules preparation method in preparation
2the percent by volume that supercritical extraction entrainer accounts for total extractant is 5%.
Above-mentioned little chaihu capsules suppresses the application in the lymphoma U937 of human tissue cell cell proliferation medicine, CO described in little chaihu capsules preparation method in preparation
2the extracting pressure 20MPa of supercritical extraction, 40 ℃ of temperature, CO
2flow 2ml/g crude drug min, extraction time 160min.
The application of above-mentioned little chaihu capsules in preparation rising erythrocyte medicine, little chaihu capsules is by Radix Bupleuri 445g, processed with Rhizoma Zingiberis Recens Rhizoma Pinelliae 222g, Radix Scutellariae 167g, Radix Codonopsis 167g, Radix Glycyrrhizae 167g, Rhizoma Zingiberis Recens 167g, Fructus Jujubae 167g makes as crude drug, preparation method is comprised of the following step: get Radix Bupleuri 445g, processed with Rhizoma Zingiberis Recens Rhizoma Pinelliae 222g, Radix Scutellariae 167g, Radix Codonopsis 167g, Radix Glycyrrhizae 167g, Rhizoma Zingiberis Recens 167g, Fructus Jujubae 167g joins in CO2 supercritical extraction device, ethanol is as entrainer, the percent by volume that entrainer accounts for total extractant is 4-6%, extracting pressure 15-30MPa, temperature 30-50 ℃, CO2 flow 1-3m1/g crude drug min, extraction time 150-180min, obtain supercritical extract, the thick paste that while being condensed into 80 ℃, relative density is 1.20-1.25, add starch, mix, dry, porphyrize, granulation, dry, make 1000 capsules, obtain.
The application of above-mentioned little chaihu capsules in preparation rising erythrocyte medicine, CO described in little chaihu capsules preparation method
2the percent by volume that supercritical extraction entrainer accounts for total extractant is 5%.
The application of above-mentioned little chaihu capsules in preparation rising erythrocyte medicine, CO described in little chaihu capsules preparation method
2the extracting pressure 20MPa of supercritical extraction, 40 ℃ of temperature, CO
2flow 2ml/g crude drug min, extraction time 160min.
In prior art, every sheet 0.4g of former little chaihu capsules, each 4,3 times on the one, every the 0.4g of little chaihu capsules that adopts the present invention to be prepared into, but the medical material amount containing is approximately original 2 times, therefore only needs 2 at every turn, within 1st, take 3 times, greatly reduced dose having under the condition of more active component.
The comparison of saikoside a content in little chaihu capsules prepared by test one, distinct methods
1, instrument and the little chaihu capsules of reagent the present invention: press embodiment 3 methods preparations, get Radix Bupleuri 445g, processed with Rhizoma Zingiberis Recens Rhizoma Pinelliae 222g, Radix Scutellariae 167g, Radix Codonopsis 167g, Radix Glycyrrhizae 167g, Rhizoma Zingiberis Recens 167g, Fructus Jujubae 167g and join CO
2in supercritical extraction device, ethanol is as entrainer, and the percent by volume that entrainer accounts for total extractant is 5%, extracting pressure 20MPa, 40 ℃ of temperature, CO
2flow 2m1/g crude drug min, extraction time 160min, obtains supercritical extract, and the thick paste that while being condensed into 80 ℃, relative density is 1.22, adds starch, mixes, dry, porphyrize, granulation, dry, make 1000 capsules, obtain.Agilent1200 high performance liquid chromatograph; METTLER AE240 electronic analytical balance; Saikoside a standard substance (Nat'l Pharmaceutical & Biological Products Control Institute).
2, method
Chromatographic condition and system suitability: with octadecylsilane chemically bonded silica, be filler; Methanol-water (66:34) is mobile phase; Detection wavelength is 204nm.Number of theoretical plate is pressed saikoside a peak and is calculated, and should be not less than 3000.
The preparation of reference substance solution: precision takes at 60 ℃ of drying under reduced pressure saikoside a reference substance of 4 hours appropriate, adds methanol and makes every 1ml containing the solution of 18 μ g, obtains.
The preparation of need testing solution: get the little chaihu capsules of the present invention and former little chaihu capsules, porphyrize, mixes, and gets 1g, accurately weighed, and precision adds 70% ethanol 20ml, close plug, supersound process 10 minutes, centrifugal, get supernatant, obtain.
Algoscopy is accurate reference substance solution and each 20 μ l of need testing solution of drawing respectively, and injection liquid chromatography, measures, and obtains.
3, result
Result shows, in the little chaihu capsules of the present invention, the content of saikoside a is 0.36mg/ grain; And the content of saikoside a is 0.17mg/ grain in former little chaihu capsules, in the situation that dose reduces, saikoside a content improves a lot.
Above-mentioned research shows, the little chaihu capsules that adopts the present invention to prepare, and active constituent content is higher than the standby little chaihu capsules of ministry standard legal system, and dose can reduce.
The specific embodiment
Form by the following examples, foregoing of the present invention is described in further detail again, but this should be interpreted as to the scope of the above-mentioned theme of the present invention only limits to following example, all technology realizing based on foregoing of the present invention all belong to scope of the present invention.
Embodiment 1
Getting Radix Bupleuri 445g, processed with Rhizoma Zingiberis Recens Rhizoma Pinelliae 222g, Radix Scutellariae 167g, Radix Codonopsis 167g, Radix Glycyrrhizae 167g, Rhizoma Zingiberis Recens 167g, Fructus Jujubae 167g joins in CO2 supercritical extraction device, ethanol is as entrainer, and the percent by volume that entrainer accounts for total extractant is 4%, extracting pressure 15MPa, 30 ℃ of temperature, CO
2flow 1m1/g crude drug min, extraction time 150min, obtains supercritical extract, and the thick paste that while being condensed into 80 ℃, relative density is 1.20, adds starch, mixes, dry, porphyrize, granulation, dry, make 1000 capsules, obtain.
Embodiment 2
Get Radix Bupleuri 445g, processed with Rhizoma Zingiberis Recens Rhizoma Pinelliae 222g, Radix Scutellariae 167g, Radix Codonopsis 167g, Radix Glycyrrhizae 167g, Rhizoma Zingiberis Recens 167g, Fructus Jujubae 167g and join CO
2in supercritical extraction device, ethanol is as entrainer, and the percent by volume that entrainer accounts for total extractant is 6%, extracting pressure 30MPa, temperature 50 C, CO
2flow 3m1/g crude drug min, extraction time 180min, obtains supercritical extract, and the thick paste that while being condensed into 80 ℃, relative density is 1.25, adds starch, mixes, dry, porphyrize, granulation, dry, make 1000 capsules, obtain.
Embodiment 3
Get Radix Bupleuri 445g, processed with Rhizoma Zingiberis Recens Rhizoma Pinelliae 222g, Radix Scutellariae 167g, Radix Codonopsis 167g, Radix Glycyrrhizae 167g, Rhizoma Zingiberis Recens 167g, Fructus Jujubae 167g and join CO
2in supercritical extraction device, ethanol is as entrainer, and the percent by volume that entrainer accounts for total extractant is 5%, extracting pressure 20MPa, 40 ℃ of temperature, CO
2flow 2m1/g crude drug min, extraction time 160min, obtains supercritical extract, and the thick paste that while being condensed into 80 ℃, relative density is 1.22, adds starch, mixes, dry, porphyrize, granulation, dry, make 1000 capsules, obtain.
Test two: little chaihu capsules suppresses the experimentation data of U937 cell proliferation
1 experiment material
1.1 experiment cell strains
The lymphoma U937 of human tissue cell cell, Nanjing Medical University's laboratory cell bank, DMEM+10%FBS cellar culture.
1.2 Experimental agents
Drugs: the little chaihu capsules of the present invention: press embodiment 3 method preparations.
Medicinal liquid liquid storage: take the little chaihu capsules of 100mg, be dissolved in 5ml dehydrated alcohol, 0.2 μ m filter filters, 500 μ ldoff pipe subpackages ,-20 ℃ of storages, 0.2 μ m filter filters dehydrated alcohol in order to the use of matched group simultaneously.The control sample of preparing Radix Bupleuri by same method.
1.3 experiment reagent
DMEM (Cat.No.12100-061Lot.No.758137 of GIBCO company); Hyclone (Lot.No.100419 of Tian Hang bio tech ltd, Zhejiang); NaHCO3 (Cat.No.11810-033Lot.No.1088387 of Shanghai Jiu Yi chemical reagent company limited); Trypsin (AMRESCO company lot number: 2010/04); EDTA (AMRESCO company lot number: 2009/10); Penicillin G Sodium Salt (AMRESCO company lot number: 2010242); Streptomycin Sulfate (AMRESCO company lot number: 2010382); Dehydrated alcohol (Nanjing Chemistry Reagent Co., Ltd.'s lot number: 080310182); MTT (Biosharp lot number: 0793); PBS (laboratory autogamy);
1.4 experiment equipment
Lycra inverted microscope (German Leica model: DM1L); Visible-ultraviolet light microwell plate detector (U.S. MD company model: SPECTRA MAX190); CO2 incubator (FORMA model: 3111); (safe and sound company of Su Jing group manufactures model to super-clean bench: SW-CJ-ZFD); Pure water instrument (U.S. Spring company model: S/N020579); Accurate pipettor (French Gilson Inc model: P2); Electronic balance (German Sai Duolisi company limited model: BT323S); Full-automatic high-pressure autoclave (Japanese SANYO company model: MLS-3020); Table electrothermal air dry oven (Shanghai accurate experimental facilities company model: DHG9123A); Refrigerator (Siemens Company's model: KG18V21TI); Liquid nitrogen container (CBS model: 2001); Low speed centrifuge (Anting Scientific Instrument Factory, Shanghai's model: KA-1000); 0.2 μ m filter (MILLIPORE model: SLGP033RB); 10cm culture dish (NEST company), 96 well culture plates (NEST company); Cell counting count board; Centrifuge tube, pipet, Tips are some.
2 experimental techniques
1) U937 cell carries out cellar culture (10cm culture dish) with DMEM+10%FBS in 37 ℃, 5%CO2, when Growth of Cells is during to logarithmic (log) phase, collecting cell, discards culture fluid, PBS fine laundering 3 times, add 3ml0.25% trypsin-0.04%EDTA, after 37 ℃ of digestion 2min, add wherein 5ml complete medium neutralization reaction, after piping and druming cell, proceeded in centrifuge tube, the centrifugal 5min of 1000rpm, adjusts 3 * 104/ml of concentration of cell suspension.
2) cell kind is entered in 96 well culture plates, every hole adds cell suspension 180 μ l, culture plate put into cell culture incubator (37 ℃, 5%CO2) cellar culture.
3) according to Growth of Cells situation, generally grow to 50%-70%, add the control sample of little chaihu capsules solution or Radix Bupleuri, continue to cultivate 24h.
4) after 24h, add 20 μ l MTT solution (5mg/ml, i.e. 0.5%MTT), continue to cultivate 4h.
5) after 4h, buckle method is removed supernatant, with absorbent paper, pats dry gently, and every hole adds 200 μ l dimethyl sulfoxide, puts low-speed oscillation 10min on shaking table, and crystal is fully dissolved.At enzyme-linked immunosorbent assay instrument 490nm place, measure the light absorption value in each hole.
6) background (do not add cell, only add culture fluid) is set simultaneously, control wells (the medicine dissolution medium of cell, same concentrations, culture fluid, MTT, dimethyl sulfoxide), sets 6 multiple holes for every group.
7) result represents the suppression ratio of cell with medicine:
Cell increment suppression ratio (%)=(control wells OD value-dosing holes OD value)/control wells OD value * 100%.Experiment repeats 3 times.
3 statistical dispositions
Adopt correlation analysis and Student t check in Microsoft Excel2003 software, data represent with mean ± S.D..
4 experimental results
Statistical result showed after mtt assay experiment, with matched group comparison, when dosage reaches 5mg/ml, to U937 cell inhibitory effect variant (P<0.05), dosage this difference when 10mg/ml has significance (P<0.01), has utmost point significant difference (P<0.001) when dosage reaches 15-20mg/ml.
The little chaihu capsules of table 1 is on U937 cell inhibitory effect impact research
Note: with matched group comparison, * P<0.01; * P<0.001, with the comparison of Radix Bupleuri group,
#p<0.01;
##p<0.001
5 experiment conclusion
Little chaihu capsules can suppress U937 cell proliferation, reduces the Growth of Cells number of U937 cell, and this effect is dose dependent, and compares and also have significant difference with single Radix Bupleuri group.
Test three: the little chaihu capsules of the present invention is on the erythrocytic impact that raises
1, animal: healthy ICR mice, male and female half and half, heavy 18-22g, is provided production licence number SCXK (Soviet Union) 2002-0031 by Nanjing Medical University's animal center.
2, medicine and reagent: press the little chaihu capsules of above-described embodiment 3 preparation the present invention.Cyclophosphamide for injection, the accurate word H14023686 of traditional Chinese medicines, production unit: Shanxi Powerdone Pharmaceutical Co., Ltd., lot number is: 20131210.
3, process of the test: get 40 of ICR mices, be divided into 4 groups (n=10), be i.e. normal saline group, modeling group, 1.2mg/kg group and 0.6mg/kg group, oral administration, every day 1 time.0th, except normal saline group, other respectively organized mice lumbar injection cycli phosphate amine 80mg/kg respectively, then continued administration 3 days on 5th, 10.1h after last administration, gets blood from eye socket venous plexus, surveys erythrocyte, gets femur bone marrow counting number of nucleated cells.
With the comparison of normal saline group: * p<0.05**p<0.01
Result shows, with the comparison of normal saline group, cycli phosphate amine can make mouse bone marrow cells damage, causes peripheral blood cells to decline, and little chaihu capsules group and model group comparison, all can obviously resist the red decline of cycli phosphate amine induced mice.
Claims (9)
1. the preparation method of a little chaihu capsules, by Radix Bupleuri 445g, processed with Rhizoma Zingiberis Recens Rhizoma Pinelliae 222g, Radix Scutellariae 167g, Radix Codonopsis 167g, Radix Glycyrrhizae 167g, Rhizoma Zingiberis Recens 167g, Fructus Jujubae 167g, as crude drug, made, it is characterized in that described method is comprised of the following step: Radix Bupleuri 445g, processed with Rhizoma Zingiberis Recens Rhizoma Pinelliae 222g, Radix Scutellariae 167g, Radix Codonopsis 167g, Radix Glycyrrhizae 167g, Rhizoma Zingiberis Recens 167g, Fructus Jujubae 167g join CO
2in supercritical extraction device, ethanol is as entrainer, and the percent by volume that entrainer accounts for total extractant is 4-6%, extracting pressure 15-30MPa, temperature 30-50 ℃, CO
2flow 1-3m1/g crude drug min, extraction time 150-180min, obtains supercritical extract, and the thick paste that while being condensed into 80 ℃, relative density is 1.20-1.25, adds starch, mixes, dry, porphyrize, granulation, dry, make 1000 capsules, obtain.
2. a kind of preparation method of little chaihu capsules according to claim 1, is characterized in that described CO
2the percent by volume that supercritical extraction entrainer accounts for total extractant is 5%.
3. a kind of preparation method of little chaihu capsules according to claim 1, is characterized in that described CO
2the extracting pressure 20MPa of supercritical extraction, 40 ℃ of temperature, CO
2flow 2ml/g crude drug min, extraction time 160min.
4. a kind of little chaihu capsules suppresses the application in the lymphoma U937 of human tissue cell cell proliferation medicine in preparation according to claim 1, it is characterized in that little chaihu capsules is by Radix Bupleuri 445g, processed with Rhizoma Zingiberis Recens Rhizoma Pinelliae 222g, Radix Scutellariae 167g, Radix Codonopsis 167g, Radix Glycyrrhizae 167g, Rhizoma Zingiberis Recens 167g, Fructus Jujubae 167g makes as crude drug, preparation method is comprised of the following step: get Radix Bupleuri 445g, processed with Rhizoma Zingiberis Recens Rhizoma Pinelliae 222g, Radix Scutellariae 167g, Radix Codonopsis 167g, Radix Glycyrrhizae 167g, Rhizoma Zingiberis Recens 167g, Fructus Jujubae 167g joins in CO2 supercritical extraction device, ethanol is as entrainer, the percent by volume that entrainer accounts for total extractant is 4-6%, extracting pressure 15-30MPa, temperature 30-50 ℃, CO2 flow 1-3m1/g crude drug min, extraction time 150-180min, obtain supercritical extract, the thick paste that while being condensed into 80 ℃, relative density is 1.20-1.25, add starch, mix, dry, porphyrize, granulation, dry, make 1000 capsules, obtain.
5. a kind of little chaihu capsules suppresses the application in the lymphoma U937 of human tissue cell cell proliferation medicine in preparation according to claim 4, it is characterized in that CO described in little chaihu capsules preparation method
2the percent by volume that supercritical extraction entrainer accounts for total extractant is 5%.
6. a kind of little chaihu capsules suppresses the application in the lymphoma U937 of human tissue cell cell proliferation medicine in preparation according to claim 4, it is characterized in that CO described in little chaihu capsules preparation method
2the extracting pressure 20MPa of supercritical extraction, 40 ℃ of temperature, CO
2flow 2ml/g crude drug min, extraction time 160min.
7. the application of little chaihu capsules according to claim 1 in preparation rising erythrocyte medicine, it is characterized in that little chaihu capsules is by Radix Bupleuri 445g, processed with Rhizoma Zingiberis Recens Rhizoma Pinelliae 222g, Radix Scutellariae 167g, Radix Codonopsis 167g, Radix Glycyrrhizae 167g, Rhizoma Zingiberis Recens 167g, Fructus Jujubae 167g makes as crude drug, preparation method is comprised of the following step: get Radix Bupleuri 445g, processed with Rhizoma Zingiberis Recens Rhizoma Pinelliae 222g, Radix Scutellariae 167g, Radix Codonopsis 167g, Radix Glycyrrhizae 167g, Rhizoma Zingiberis Recens 167g, Fructus Jujubae 167g joins in CO2 supercritical extraction device, ethanol is as entrainer, the percent by volume that entrainer accounts for total extractant is 4-6%, extracting pressure 15-30MPa, temperature 30-50 ℃, CO2 flow 1-3m1/g crude drug min, extraction time 150-180min, obtain supercritical extract, the thick paste that while being condensed into 80 ℃, relative density is 1.20-1.25, add starch, mix, dry, porphyrize, granulation, dry, make 1000 capsules, obtain.
8. the application of a kind of little chaihu capsules in preparation rising erythrocyte medicine according to claim 7, is characterized in that CO described in little chaihu capsules preparation method
2the percent by volume that supercritical extraction entrainer accounts for total extractant is 5%.
9. the application of a kind of little chaihu capsules in preparation rising erythrocyte medicine according to claim 7, is characterized in that CO described in little chaihu capsules preparation method
2the extracting pressure 20MPa of supercritical extraction, 40 ℃ of temperature, CO
2flow 2ml/g crude drug min, extraction time 160min.
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| CN106880829A (en) * | 2017-04-17 | 2017-06-23 | 张克杰 | Chinese medicine composition for treating lymphatic system dysfunction lesion |
| CN110960663A (en) * | 2019-12-21 | 2020-04-07 | 江西济民可信金水宝制药有限公司 | Preparation method of Xiaochaihu granules |
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Address after: No. 5 Jinghong Road, Changhong Community, Ala Street Office, Kunming Economic Development Zone, China (Yunnan) Pilot Free Trade Zone, Kunming City, Yunnan Province, 650217 Patentee after: YUNNAN YUNLONG PHARMACEUTICAL Co.,Ltd. Address before: No.5 Jinghong Road, Kunming Economic and Technological Development Zone, Yunnan Province Patentee before: YUNNAN YUNLONG PHARMACEUTICAL Co.,Ltd. |