CN103721136A - Preparation method and application of pharyngitis tablet - Google Patents
Preparation method and application of pharyngitis tablet Download PDFInfo
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- CN103721136A CN103721136A CN201310615918.2A CN201310615918A CN103721136A CN 103721136 A CN103721136 A CN 103721136A CN 201310615918 A CN201310615918 A CN 201310615918A CN 103721136 A CN103721136 A CN 103721136A
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- 238000002360 preparation method Methods 0.000 title claims abstract description 27
- 201000007100 Pharyngitis Diseases 0.000 title abstract description 6
- 239000003814 drug Substances 0.000 claims abstract description 27
- 238000000194 supercritical-fluid extraction Methods 0.000 claims abstract description 16
- 241000628997 Flos Species 0.000 claims abstract description 14
- 230000004663 cell proliferation Effects 0.000 claims abstract description 10
- 206010028980 Neoplasm Diseases 0.000 claims abstract description 9
- 201000011510 cancer Diseases 0.000 claims abstract description 9
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 31
- 229940079593 drug Drugs 0.000 claims description 16
- 239000010231 banlangen Substances 0.000 claims description 12
- 238000000605 extraction Methods 0.000 claims description 12
- 210000000582 semen Anatomy 0.000 claims description 12
- 206010020880 Hypertrophy Diseases 0.000 claims description 8
- 229920002472 Starch Polymers 0.000 claims description 7
- 239000000284 extract Substances 0.000 claims description 7
- 239000008187 granular material Substances 0.000 claims description 7
- 235000019698 starch Nutrition 0.000 claims description 7
- 239000008107 starch Substances 0.000 claims description 7
- 238000000034 method Methods 0.000 claims description 6
- 230000002401 inhibitory effect Effects 0.000 abstract description 3
- 241000432824 Asparagus densiflorus Species 0.000 abstract 1
- 244000012254 Canarium album Species 0.000 abstract 1
- 235000009103 Canarium album Nutrition 0.000 abstract 1
- 241000931705 Cicada Species 0.000 abstract 1
- 241000334160 Isatis Species 0.000 abstract 1
- 241000699670 Mus sp. Species 0.000 abstract 1
- 240000005790 Oroxylum indicum Species 0.000 abstract 1
- 235000012920 Oroxylum indicum Nutrition 0.000 abstract 1
- 241000405414 Rehmannia Species 0.000 abstract 1
- 210000003630 histaminocyte Anatomy 0.000 abstract 1
- 239000001525 mentha piperita l. herb oil Substances 0.000 abstract 1
- 235000019477 peppermint oil Nutrition 0.000 abstract 1
- 239000002994 raw material Substances 0.000 abstract 1
- 229960004756 ethanol Drugs 0.000 description 10
- 238000002474 experimental method Methods 0.000 description 8
- IAZDPXIOMUYVGZ-UHFFFAOYSA-N Dimethylsulphoxide Chemical compound CS(C)=O IAZDPXIOMUYVGZ-UHFFFAOYSA-N 0.000 description 4
- 239000006144 Dulbecco’s modified Eagle's medium Substances 0.000 description 3
- 230000010261 cell growth Effects 0.000 description 3
- 239000012531 culture fluid Substances 0.000 description 3
- 229960000935 dehydrated alcohol Drugs 0.000 description 3
- 239000007788 liquid Substances 0.000 description 3
- 210000003800 pharynx Anatomy 0.000 description 3
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 2
- UIIMBOGNXHQVGW-UHFFFAOYSA-M Sodium bicarbonate Chemical compound [Na+].OC([O-])=O UIIMBOGNXHQVGW-UHFFFAOYSA-M 0.000 description 2
- 239000006285 cell suspension Substances 0.000 description 2
- 239000003153 chemical reaction reagent Substances 0.000 description 2
- 239000000843 powder Substances 0.000 description 2
- 238000003860 storage Methods 0.000 description 2
- 239000006228 supernatant Substances 0.000 description 2
- 230000001629 suppression Effects 0.000 description 2
- 235000019640 taste Nutrition 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- WEEMDRWIKYCTQM-UHFFFAOYSA-N 2,6-dimethoxybenzenecarbothioamide Chemical compound COC1=CC=CC(OC)=C1C(N)=S WEEMDRWIKYCTQM-UHFFFAOYSA-N 0.000 description 1
- 206010011224 Cough Diseases 0.000 description 1
- 238000002965 ELISA Methods 0.000 description 1
- 208000003251 Pruritus Diseases 0.000 description 1
- 241001530126 Scrophularia Species 0.000 description 1
- 229920002334 Spandex Polymers 0.000 description 1
- 102000004142 Trypsin Human genes 0.000 description 1
- 108090000631 Trypsin Proteins 0.000 description 1
- 240000000377 Tussilago farfara Species 0.000 description 1
- 235000004869 Tussilago farfara Nutrition 0.000 description 1
- 239000002250 absorbent Substances 0.000 description 1
- 230000002745 absorbent Effects 0.000 description 1
- 230000000954 anitussive effect Effects 0.000 description 1
- 230000001139 anti-pruritic effect Effects 0.000 description 1
- 239000003908 antipruritic agent Substances 0.000 description 1
- 229940124584 antitussives Drugs 0.000 description 1
- 238000003556 assay Methods 0.000 description 1
- 230000010165 autogamy Effects 0.000 description 1
- FCPVYOBCFFNJFS-LQDWTQKMSA-M benzylpenicillin sodium Chemical compound [Na+].N([C@H]1[C@H]2SC([C@@H](N2C1=O)C([O-])=O)(C)C)C(=O)CC1=CC=CC=C1 FCPVYOBCFFNJFS-LQDWTQKMSA-M 0.000 description 1
- 238000004113 cell culture Methods 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 238000010219 correlation analysis Methods 0.000 description 1
- 239000013078 crystal Substances 0.000 description 1
- 230000029087 digestion Effects 0.000 description 1
- ZZVUWRFHKOJYTH-UHFFFAOYSA-N diphenhydramine Chemical compound C=1C=CC=CC=1C(OCCN(C)C)C1=CC=CC=C1 ZZVUWRFHKOJYTH-UHFFFAOYSA-N 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 239000012738 dissolution medium Substances 0.000 description 1
- 231100000673 dose–response relationship Toxicity 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000005469 granulation Methods 0.000 description 1
- 230000003179 granulation Effects 0.000 description 1
- 239000012535 impurity Substances 0.000 description 1
- 230000007803 itching Effects 0.000 description 1
- 238000004900 laundering Methods 0.000 description 1
- 230000031700 light absorption Effects 0.000 description 1
- 210000004072 lung Anatomy 0.000 description 1
- 230000014759 maintenance of location Effects 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 239000012567 medical material Substances 0.000 description 1
- 239000002609 medium Substances 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 238000006386 neutralization reaction Methods 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
- 230000010355 oscillation Effects 0.000 description 1
- 231100000614 poison Toxicity 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 230000005180 public health Effects 0.000 description 1
- 229910000030 sodium bicarbonate Inorganic materials 0.000 description 1
- 235000017557 sodium bicarbonate Nutrition 0.000 description 1
- 239000004759 spandex Substances 0.000 description 1
- 238000001228 spectrum Methods 0.000 description 1
- 239000007921 spray Substances 0.000 description 1
- 229960002385 streptomycin sulfate Drugs 0.000 description 1
- 238000009495 sugar coating Methods 0.000 description 1
- 239000003440 toxic substance Substances 0.000 description 1
- 239000012588 trypsin Substances 0.000 description 1
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- Medicines Containing Plant Substances (AREA)
Abstract
The invention provides a preparation method of a pharyngitis tablet. The pharyngitis tablet is prepared by the following steps: using the following components as raw materials: 120 g of radix scrophulariae, 90 g of radix stemonae, 90 g of asparagus fern, 90 g of moutan bark, 90 g of radix ophiopogonis, 90 g of flos farfarae, 30 g of oroxylum indicum, 90 g of rehmannia, 150 g of isatis roots, 90 g of Chinese olive, 30 g of periostracum cicada and 0.3 g of peppermint oil, and adopting the supercritical extraction. By adopting the technology, the content of the pharyngitis tablet is improved greatly, and the dose is reduced. The invention further provides application of the pharyngitis tablet in a medicine for inhibiting mice mast cell cancer P815 cell proliferation.
Description
Technical field
The present invention relates to Chinese medicine preparation technical field, be specifically related to a kind of preparation method and application of Yanyan slice.
Background technology
Yanyan slice is recorded in Ministry of Public Health standard WS3-B-0331-90, prescription is Radix Scrophulariae 120g, Radix Stemonae 90g, Radix Asparagi 90g, Cortex Moutan 90g, Radix Ophiopogonis 90g, Flos Farfarae 90g, Semen Oroxyli 30g, Radix Rehmanniae 90g, Radix Isatidis 150g, Fructus Canarii 90g, Periostracum Cicadae 30g, Oleum menthae 0.3g, above 12 tastes, except Oleum menthae, Flos Farfarae is ground into fine powder, sieves; Ten tastes such as all the other Radix Scrophulariaes decoct with water secondary, and 3 hours for the first time, 2 hours for the second time, collecting decoction, standing 24 hours, get supernatant, be condensed into thick paste, mix with Common Coltsfoot Flower, dry, granulation, spray, with Oleum menthae, mixes, and is pressed into 1000, and sugar coating, obtains.Energy nourishing YIN and moistening the lung, heat-clearing and toxic substances removing, clearing throat, antitussive is antipruritic.The dry pharynx causing for chronic pharyngitis, itching throat, the diseases such as irritable cough.
In prior art, not yet there is Yanyan slice at the report that adopts supercritical technology aspect extraction preparation, and the method that adopts decocting to boil and beat powder, technique is coarse, backward, and impurity is many, causes patient's consumption excessive, be inconvenient to take, had a strong impact on this product and applied clinically.
Summary of the invention
Goal of the invention: in order to address the above problem, the object of the present invention is to provide a kind of preparation method of Yanyan slice.
Another object of the present invention is to provide a kind of Yanyan slice to suppress the application in mouse hypertrophy cell cancer P815 cell proliferation medicine in preparation.
Technical scheme: the object of the invention is to realize by following scheme:
A kind of preparation method of Yanyan slice, by Radix Scrophulariae 120g, Radix Stemonae 90g, Radix Asparagi 90g, Cortex Moutan 90g, Radix Ophiopogonis 90g, Flos Farfarae 90g, Semen Oroxyli 30g, Radix Rehmanniae 90g, Radix Isatidis 150g, Fructus Canarii 90g, Periostracum Cicadae 30g, Oleum menthae 0.3g makes as crude drug, described method is comprised of the following step: get Radix Scrophulariae 120g, Radix Stemonae 90g, Radix Asparagi 90g, Cortex Moutan 90g, Radix Ophiopogonis 90g, Flos Farfarae 90g, Semen Oroxyli 30g, Radix Rehmanniae 90g, Radix Isatidis 150g, Fructus Canarii 90g, Periostracum Cicadae 30g, Oleum menthae 0.3g joins in CO2 supercritical extraction device, ethanol is as entrainer, the percent by volume that entrainer accounts for total extractant is 4-6%, extracting pressure 15-30MPa, temperature 30-50 ℃, CO
2flow 1-3m1/g crude drug min, extraction time 150-180min, obtains supercritical extract, adds starch, 70% ethanol granule processed, dry, tabletting, makes 500, every heavy 0.25g.
The preparation method of above-mentioned a kind of Yanyan slice, described CO
2the percent by volume that supercritical extraction entrainer accounts for total extractant is 5%.
The preparation method of above-mentioned a kind of Yanyan slice, described CO
2the extracting pressure 20MPa of supercritical extraction, 40 ℃ of temperature, CO
2flow 2ml/g crude drug min, extraction time 160min.
Above-mentioned a kind of Yanyan slice suppresses the application in mouse hypertrophy cell cancer P815 cell proliferation medicine in preparation, Yanyan slice is by Radix Scrophulariae 120g, Radix Stemonae 90g, Radix Asparagi 90g, Cortex Moutan 90g, Radix Ophiopogonis 90g, Flos Farfarae 90g, Semen Oroxyli 30g, Radix Rehmanniae 90g, Radix Isatidis 150g, Fructus Canarii 90g, Periostracum Cicadae 30g, Oleum menthae 0.3g makes as crude drug, preparation method is comprised of the following step: get Radix Scrophulariae 120g, Radix Stemonae 90g, Radix Asparagi 90g, Cortex Moutan 90g, Radix Ophiopogonis 90g, Flos Farfarae 90g, Semen Oroxyli 30g, Radix Rehmanniae 90g, Radix Isatidis 150g, Fructus Canarii 90g, Periostracum Cicadae 30g, Oleum menthae 0.3g, join CO
2in supercritical extraction device, ethanol is as entrainer, and the percent by volume that entrainer accounts for total extractant is 4-6%, extracting pressure 15-30MPa, temperature 30-50 ℃, CO
2flow 1-3m1/g crude drug min, extraction time 150-180min, obtains supercritical extract, adds starch, 70% ethanol granule processed, dry, tabletting, makes 500, every heavy 0.25g.
Above-mentioned a kind of Yanyan slice suppresses the application in mouse hypertrophy cell cancer P815 cell proliferation medicine in preparation, and the percent by volume that the entrainer of CO2 supercritical extraction described in the preparation method of Yanyan slice accounts for total extractant is 5%.
Above-mentioned a kind of Yanyan slice suppresses the application in mouse hypertrophy cell cancer P815 cell proliferation medicine, the extracting pressure 20MPa of CO2 supercritical extraction described in the preparation method of Yanyan slice, 40 ℃ of temperature, CO2 flow 2ml/g crude drug min, extraction time 160min in preparation.
In prior art, every 0.25g of Yanyan slice, each 5,3 times on the one, the every 0.25g of Yanyan slice that adopts the present invention to be prepared into, but the medical material amount containing is original 2 times, therefore only need 3 at every turn, within 1st, take 3 times, under the condition with more active component, greatly reduced dose.
The specific embodiment
Form by the following examples, foregoing of the present invention is described in further detail again, but this should be interpreted as to the scope of the above-mentioned theme of the present invention only limits to following example, all technology realizing based on foregoing of the present invention all belong to scope of the present invention.
Embodiment 1
Getting Radix Scrophulariae 120g, Radix Stemonae 90g, Radix Asparagi 90g, Cortex Moutan 90g, Radix Ophiopogonis 90g, Flos Farfarae 90g, Semen Oroxyli 30g, Radix Rehmanniae 90g, Radix Isatidis 150g, Fructus Canarii 90g, Periostracum Cicadae 30g, Oleum menthae 0.3g joins in CO2 supercritical extraction device, ethanol is as entrainer, the percent by volume that entrainer accounts for total extractant is 4%, extracting pressure 15MPa, 30 ℃ of temperature, CO
2flow 1m1/g crude drug min, extraction time 150min, obtains supercritical extract, adds starch, 70% ethanol granule processed, dry, tabletting, makes 500, every heavy 0.25g.
Embodiment 2
Get Radix Scrophulariae 120g, Radix Stemonae 90g, Radix Asparagi 90g, Cortex Moutan 90g, Radix Ophiopogonis 90g, Flos Farfarae 90g, Semen Oroxyli 30g, Radix Rehmanniae 90g, Radix Isatidis 150g, Fructus Canarii 90g, Periostracum Cicadae 30g, Oleum menthae 0.3g and join CO
2in supercritical extraction device, ethanol is as entrainer, and the percent by volume that entrainer accounts for total extractant is 6%, extracting pressure 30MPa, temperature 50 C, CO
2flow 3m1/g crude drug min, extraction time 180min, obtains supercritical extract, adds starch, 70% ethanol granule processed, dry, tabletting, makes 500, every heavy 0.25g.
Embodiment 3
Get Radix Scrophulariae 120g, Radix Stemonae 90g, Radix Asparagi 90g, Cortex Moutan 90g, Radix Ophiopogonis 90g, Flos Farfarae 90g, Semen Oroxyli 30g, Radix Rehmanniae 90g, Radix Isatidis 150g, Fructus Canarii 90g, Periostracum Cicadae 30g, Oleum menthae 0.3g and join CO
2in supercritical extraction device, ethanol is as entrainer, and the percent by volume that entrainer accounts for total extractant is 5%, extracting pressure 20MPa, 40 ℃ of temperature, CO
2flow 2m1/g crude drug min, extraction time 160min, obtains supercritical extract, adds starch, 70% ethanol granule processed, dry, tabletting, makes 500, every heavy 0.25g.
Embodiment 4: Yanyan slice suppresses the experimentation data of P815 cell proliferation
1 experiment material
1.1 experiment cell strains
Mouse hypertrophy cell cancer P815 cell, Nanjing Medical University's laboratory cell bank, DMEM+10%FBS cellar culture.
1.2 Experimental agents
Drugs: Yanyan slice of the present invention: press embodiment 3 method preparations.
Medicinal liquid liquid storage: take 100mg Yanyan slice, be dissolved in 5ml dehydrated alcohol, 0.2 μ m filter filters, 500 μ l doff pipe subpackages ,-20 ℃ of storages, 0.2 μ m filter filters the use of dehydrated alcohol in order to matched group simultaneously.
1.3 experiment reagent
The Cat.No.12100-061Lot.No.758137 of DMEM(GIBCO company); Hyclone (Lot.No.100419 of Tian Hang bio tech ltd, Zhejiang); NaHCO3(Shanghai hundred million Cat.No.11810-033Lot.No.1088387 of chemical reagent company limited of a specified duration); Trypsin(AMRESCO company lot number: 2010/04); EDTA(AMRESCO company lot number: 2009/10); Penicillin G Sodium Salt(AMRESCO company lot number: 2010242); Streptomycin Sulfate(AMRESCO company lot number: 2010382); Dehydrated alcohol (Nanjing Chemistry Reagent Co., Ltd.'s lot number: 080310182); MTT (Biosharp lot number: 0793); PBS(laboratory autogamy);
1.4 experiment equipment
Lycra inverted microscope (German Leica model: DM1L); Visible-ultraviolet light microwell plate detector (U.S. MD company model: SPECTRA MAX190); CO2 incubator (FORMA model: 3111); Super-clean bench (safe and sound company of Su Jing group manufactures model: SW-CJ-ZFD); Pure water instrument (U.S. Spring company model: S/N020579); Accurate pipettor (French Gilson Inc model: P2); Electronic balance (German Sai Duolisi company limited model: BT323S); Full-automatic high-pressure autoclave (Japanese SANYO company model: MLS-3020); Table electrothermal air dry oven (Shanghai accurate experimental facilities company model: DHG9123A); Refrigerator (Siemens Company's model: KG18V21TI); Liquid nitrogen container (CBS model: 2001); Low speed centrifuge (Anting Scientific Instrument Factory, Shanghai's model: KA-1000); 0.2 μ m filter (MILLIPORE model: SLGP033RB); 10cm culture dish (NEST company), 96 well culture plates (NEST company); Cell counting count board; Centrifuge tube, pipet, Tips are some.
2 experimental techniques
1) P815 cell carries out cellar culture (10cm culture dish) with DMEM+10%FBS in 37 ℃, 5%CO2, when Growth of Cells is during to logarithmic (log) phase, collecting cell, discards culture fluid, PBS fine laundering 3 times, add 3ml0.25% trypsin-0.04%EDTA, after 37 ℃ of digestion 2min, add wherein 5ml complete medium neutralization reaction, after piping and druming cell, proceeded in centrifuge tube, the centrifugal 5min of 1000rpm, adjusts 3 × 104/ml of concentration of cell suspension.
2) cell kind is entered in 96 well culture plates, every hole adds cell suspension 180 μ l, and culture plate is put into cell culture incubator (37 ℃, 5%CO2) cellar culture.
3) according to Growth of Cells situation, generally grow to 50%-70%, add Yanyan slice solution, continue to cultivate 24h.
4) after 24h, add 20 μ l MTT solution (5mg/ml, i.e. 0.5%MTT), continue to cultivate 4h.
5) after 4h, buckle method is removed supernatant, pats dry gently with absorbent paper, and every hole adds 200 μ l dimethyl sulfoxide, puts low-speed oscillation 10min on shaking table, and crystal is fully dissolved.At enzyme-linked immunosorbent assay instrument 490nm place, measure the light absorption value in each hole.
6) background (do not add cell, only add culture fluid) is set simultaneously, control wells (the medicine dissolution medium of cell, same concentrations, culture fluid, MTT, dimethyl sulfoxide), sets 6 multiple holes for every group.
7) result represents the suppression ratio of cell with medicine:
Cell increment suppression ratio (%)=(control wells OD value-dosing holes OD value)/control wells OD value × 100%.Experiment repeats 3 times.
3 statistical dispositions
Adopt correlation analysis and Student t check in Microsoft Excel2003 software, data represent with mean ± S.D..
4 experimental results
Statistical result showed after mtt assay experiment, with matched group comparison, when dosage reaches 5mg/ml, to P815 cell inhibitory effect variant (P<0.05), dosage this difference when 10mg/ml has significance (P<0.01), has utmost point significant difference (P<0.001) when dosage reaches 15-20mg/ml.
Table 1 Yanyan slice is on P815 cell inhibitory effect impact research
Note: with matched group comparison, * P<0.01; * P<0.001
5 experiment conclusion
Yanyan slice can suppress P815 cell proliferation, reduces the Growth of Cells number of P815 cell, and this effect is dose dependent.
Claims (6)
1. the preparation method of a Yanyan slice, by Radix Scrophulariae 120g, Radix Stemonae 90g, Radix Asparagi 90g, Cortex Moutan 90g, Radix Ophiopogonis, 90g, Flos Farfarae 90g, Semen Oroxyli 30g, Radix Rehmanniae 90g, Radix Isatidis 150g, Fructus Canarii 90g, Periostracum Cicadae 30g, Oleum menthae 0.3g make as crude drug, it is characterized in that described method is comprised of the following step: get Radix Scrophulariae 120g, Radix Stemonae 90g, Radix Asparagi 90g, Cortex Moutan 90g, Radix Ophiopogonis 90g, Flos Farfarae 90g, Semen Oroxyli 30g, Radix Rehmanniae 90g, Radix Isatidis 150g, Fructus Canarii 90g, Periostracum Cicadae 30g, Oleum menthae 0.3g, join CO
2in supercritical extraction device, ethanol is as entrainer, and the percent by volume that entrainer accounts for total extractant is 4-6%, extracting pressure 15-30MPa, temperature 30-50 ℃, CO
2flow 1-3m1/g crude drug min, extraction time 150-180min, obtains supercritical extract, adds starch, 70% ethanol granule processed, dry, tabletting, makes 500, every heavy 0.25g.
2. a kind of preparation method of Yanyan slice according to claim 1, is characterized in that described CO
2the percent by volume that supercritical extraction entrainer accounts for total extractant is 5%.
3. a kind of preparation method of Yanyan slice according to claim 1, is characterized in that described CO
2the extracting pressure 20MPa of supercritical extraction, 40 ℃ of temperature, CO
2flow 2ml/g crude drug min, extraction time 160min.
4. a kind of Yanyan slice suppresses the application in mouse hypertrophy cell cancer P815 cell proliferation medicine in preparation according to claim 1, it is characterized in that Yanyan slice is by Radix Scrophulariae 120g, Radix Stemonae 90g, Radix Asparagi 90g, Cortex Moutan 90g, Radix Ophiopogonis 90g, Flos Farfarae 90g, Semen Oroxyli 30g, Radix Rehmanniae 90g, Radix Isatidis 150g, Fructus Canarii 90g, Periostracum Cicadae 30g, Oleum menthae 0.3g makes as crude drug, preparation method is comprised of the following step: get Radix Scrophulariae 120g, Radix Stemonae 90g, Radix Asparagi 90g, Cortex Moutan 90g, Radix Ophiopogonis 90g, Flos Farfarae 90g, Semen Oroxyli 30g, Radix Rehmanniae 90g, Radix Isatidis 150g, Fructus Canarii 90g, Periostracum Cicadae 30g, Oleum menthae 0.3g, join CO
2in supercritical extraction device, ethanol is as entrainer, and the percent by volume that entrainer accounts for total extractant is 4-6%, extracting pressure 15-30MPa, temperature 30-50 ℃, CO
2flow 1-3m1/g crude drug min, extraction time 150-180min, obtains supercritical extract, adds starch, 70% ethanol granule processed, dry, tabletting, makes 500, every heavy 0.25g.
5. a kind of Yanyan slice suppresses the application in mouse hypertrophy cell cancer P815 cell proliferation medicine in preparation according to claim 4, it is characterized in that CO described in the preparation method of Yanyan slice
2the percent by volume that supercritical extraction entrainer accounts for total extractant is 5%.
6. a kind of Yanyan slice suppresses the application in mouse hypertrophy cell cancer P815 cell proliferation medicine in preparation according to claim 4, it is characterized in that CO described in the preparation method of Yanyan slice
2the extracting pressure 20MPa of supercritical extraction, 40 ℃ of temperature, CO
2flow 2ml/g crude drug min, extraction time 160min.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104922227A (en) * | 2015-05-28 | 2015-09-23 | 吉林省正和药业集团股份有限公司 | Preparation method of Yuanhe stomach tablet and application |
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2013
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Non-Patent Citations (2)
| Title |
|---|
| 中华人民共和国卫生部药典委员会: "《中华人民共和国卫生部药品标准中药成方制剂第二册》", 31 December 1990, article "咽炎片", pages: 161 * |
| 廖传华等: "《超临界CO2流体萃取技术——工艺开发及其应用》", 31 July 2004, article "复方中药超临界CO2流体提取及药学研究", pages: 245 - 2-6 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104922227A (en) * | 2015-05-28 | 2015-09-23 | 吉林省正和药业集团股份有限公司 | Preparation method of Yuanhe stomach tablet and application |
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