CN104922227A - Preparation method of Yuanhe stomach tablet and application - Google Patents
Preparation method of Yuanhe stomach tablet and application Download PDFInfo
- Publication number
- CN104922227A CN104922227A CN201510283362.0A CN201510283362A CN104922227A CN 104922227 A CN104922227 A CN 104922227A CN 201510283362 A CN201510283362 A CN 201510283362A CN 104922227 A CN104922227 A CN 104922227A
- Authority
- CN
- China
- Prior art keywords
- preparation
- radix
- sheet
- unit
- crude drug
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Medicines Containing Plant Substances (AREA)
Abstract
The invention belongs to the field of Chinese medicinal preparations, and particularly provides a preparation method of a Yuanhe stomach tablet. The Yuanhe stomach tablet is prepared from the following bulk pharmaceuticals: 300g of rhubarb, 300g of rough gentian, 300g of costus root, 200g of corydalis tuber, 25g of mint, 200g of liquoric root, 20g of clove and 600g of sodium bicarbonate. The Yuanhe stomach tablet is prepared through hydrochloric acid extraction and supercritical extraction, so that the content is increased greatly, the dosage is reduced, and the tablet hardness is enhanced. The invention further provides application of the Yuanhe stomach tablet to preparation of medicine for restraining proliferation of mice mastocytoma P815 cells.
Description
Technical field
The present invention relates to technical field of traditional Chinese medicine preparation, be specifically related to preparation method and the application of a kind of unit and positive stomach sheet.
Background technology
First and positive stomach sheet is recorded in national drug food Surveillance Authority standard, prescription is Radix Et Rhizoma Rhei 300g, Radix Gentianae 300g, Radix Aucklandiae 300g, Rhizoma Corydalis 200g, Herba Menthae 25g, Radix Glycyrrhizae 200g, Flos Caryophylli 20g, sodium bicarbonate 600g, above eight tastes, get the Radix Aucklandiae, Flos Caryophylli, Herba Menthae extraction by steam distillation volatile oil 6 hours, distillation rear solution and volatile oil for subsequent use, medicinal residues decoct with water 2 hours, and filter, filtrate is for subsequent use; Radix Et Rhizoma Rhei, Radix Gentianae, Rhizoma Corydalis, Radix Glycyrrhizae decoct with water secondary, each 2 hours, and gradation filters, merging filtrate, merges with the aqueous solution after above-mentioned distillation and decocting liquid, is evaporated to the thick paste that relative density is 1.30-1.35 (60 ~ 70 DEG C), dry, pulverize into fine powder, add sodium bicarbonate powder, mixing, a small amount of dissolve with ethanol of above-mentioned volatile oil, evenly sprays into, mixing, tabletting, makes 1000, to obtain final product.Energy normalizing the stomach by guiding QI downward, relieving gastric hyperacidity to alleviate stomachache.For stomachache, distension and fullness in the abdomen, eating accumulation, inappetence, flatulence acid regurgitation, peptic ulcer.
In prior art, unit and positive stomach sheet is not yet had to adopt supercritical technology in extraction preparation, the report of copolyvidone is used in preparation process, and adopt the method for soak by water, technique is coarse, backward, and impurity is many, causes patient's consumption excessive, be inconvenient to take, had a strong impact on this product and applied clinically.
In prior art, the every sheet 0.75g of first and positive stomach sheet, each 1,3 times on the one, the unit adopting the present invention to be prepared into and the every sheet 0.75g of positive stomach sheet, but the medical material amount of same weight produced 1000 originally, present production 800, illustrates that the tablet drug loading of same weight increases, therefore needs 1 at every turn, within 1st, take 3 times, under same dose, there is more active component.And in prior art, use slice, thin piece too loose, be easy to split, the present invention uses copolyvidone as binding agent, can reach disintegration, also has certain hardness, is conducive to transport.
Summary of the invention
Goal of the invention: in order to solve the problem, the object of the present invention is to provide the preparation method of a kind of unit and positive stomach sheet.
Another object of the present invention is to provide unit and positive stomach sheet to suppress the application in mouse hypertrophy cell cancer P815 cell proliferation in preparation.
Technical scheme: the object of the invention is by following scheme realize:
The preparation method of a kind of unit and positive stomach sheet, by Radix Et Rhizoma Rhei 300g, Radix Gentianae 300g, Radix Aucklandiae 300g, Rhizoma Corydalis 200g, Herba Menthae 25g, Radix Glycyrrhizae 200g, Flos Caryophylli 20g, sodium bicarbonate 600g makes as crude drug, described method is made up of the following step: get Radix Et Rhizoma Rhei 300g, Radix Gentianae 300g, Radix Aucklandiae 300g, Rhizoma Corydalis 200g, Herba Menthae 25g, Radix Glycyrrhizae 200g, Flos Caryophylli 20g, join in CO2 supercritical extraction device, ethanol is as entrainer, the percent by volume that entrainer accounts for total extractant is 4-6%, extracting pressure 15-30MPa, temperature 30-50 DEG C, CO2 flow 1-3m1/g crude drug min, extraction time 150-180min, obtain supercritical extract, add sodium bicarbonate 600g, copolyvidone 20g, 70% ethanol granule, dry, tabletting, make 800, the heavy 0.75g of every sheet.
The preparation method of described unit and positive stomach sheet, the percent by volume that described CO2 supercritical extraction entrainer accounts for total extractant is 5%.
The preparation method of described unit and positive stomach sheet, the extracting pressure 20MPa of described CO2 supercritical extraction, temperature 40 DEG C, CO2 flow 2ml/g crude drug min, extraction time 160min.
Described unit and positive stomach sheet suppress the application in mouse hypertrophy cell cancer P815 cell proliferation in preparation, described method is made up of the following step: get Radix Et Rhizoma Rhei 300g, Radix Gentianae 300g, Radix Aucklandiae 300g, Rhizoma Corydalis 200g, Herba Menthae 25g, Radix Glycyrrhizae 200g, Flos Caryophylli 20g, join in CO2 supercritical extraction device, ethanol is as entrainer, the percent by volume that entrainer accounts for total extractant is 4-6%, extracting pressure 15-30MPa, temperature 30-50 DEG C, CO2 flow 1-3m1/g crude drug min, extraction time 150-180min, obtain supercritical extract, add sodium bicarbonate 600g, copolyvidone 20g, 70% ethanol granule, dry, tabletting, make 800, the heavy 0.75g of every sheet.
Described unit and positive stomach sheet suppress the application in mouse hypertrophy cell cancer P815 cell proliferation in preparation, and it is 5% that CO2 supercritical extraction entrainer described in the preparation method of first and positive stomach sheet accounts for the percent by volume of total extractant.
Described unit and positive stomach sheet suppress the application in mouse hypertrophy cell cancer P815 cell proliferation in preparation, the extracting pressure 20MPa of CO2 supercritical extraction described in the preparation method of first and positive stomach sheet, temperature 40 DEG C, CO2 flow 2ml/g crude drug min, extraction time 160min.
Detailed description of the invention
Form by the following examples, foregoing of the present invention is described in further detail again, but this should be interpreted as that the scope of the above-mentioned theme of the present invention is only limitted to following example, all technology realized based on foregoing of the present invention all belong to scope of the present invention.
Embodiment 1: get Radix Et Rhizoma Rhei 300g, Radix Gentianae 300g, Radix Aucklandiae 300g, Rhizoma Corydalis 200g, Herba Menthae 25g, Radix Glycyrrhizae 200g, Flos Caryophylli 20g, join in CO2 supercritical extraction device, ethanol is as entrainer, the percent by volume that entrainer accounts for total extractant is 4%, extracting pressure 30MPa, temperature 30 DEG C, CO2 flow 3m1/g crude drug min, extraction time 150min, obtains supercritical extract, add sodium bicarbonate 600g, copolyvidone 20g, 70% ethanol granule, dry, tabletting, make 800, the heavy 0.75g of every sheet.
Embodiment 2: get Radix Et Rhizoma Rhei 300g, Radix Gentianae 300g, Radix Aucklandiae 300g, Rhizoma Corydalis 200g, Herba Menthae 25g, Radix Glycyrrhizae 200g, Flos Caryophylli 20g, join in CO2 supercritical extraction device, ethanol is as entrainer, the percent by volume that entrainer accounts for total extractant is 6%, extracting pressure 15MPa, temperature 50 C, CO2 flow 1m1/g crude drug min, extraction time 180min, obtains supercritical extract, add sodium bicarbonate 600g, copolyvidone 20g, 70% ethanol granule, dry, tabletting, make 800, the heavy 0.75g of every sheet.
Embodiment 3: get Radix Et Rhizoma Rhei 300g, Radix Gentianae 300g, Radix Aucklandiae 300g, Rhizoma Corydalis 200g, Herba Menthae 25g, Radix Glycyrrhizae 200g, Flos Caryophylli 20g, join in CO2 supercritical extraction device, ethanol is as entrainer, the percent by volume that entrainer accounts for total extractant is 5%, extracting pressure 20MPa, temperature 40 DEG C, CO2 flow 2m1/g crude drug min, extraction time 160min, obtains supercritical extract, add sodium bicarbonate 600g, copolyvidone 20g, 70% ethanol granule, dry, tabletting, make 800, the heavy 0.75g of every sheet.
Embodiment 4: first and positive stomach sheet suppresses the experimentation data of mouse hypertrophy cell cancer P815 cell proliferation
1 experiment material
1.1 experiment cell strains: mouse hypertrophy cell cancer P815 cell, Jilin Province just with Pharmaceutical Group Plc laboratory cell storehouse, DMEM+10%FBS cellar culture.
1.2 Experimental agents: drugs: the present invention unit and positive stomach sheet: prepare by embodiment 3 method.Medicinal liquid liquid storage: take 100mg unit and positive stomach sheet, be dissolved in 5ml dehydrated alcohol, 0.2 μm of frit, 500 μ l doff pipe subpackages ,-20 DEG C of storage, while 0.2 μm of frit dehydrated alcohol prepare against the use of matched group.
1.3 experiment reagents: DMEM (GIBCO company Cat.No.12100-061 Lot.No.758137); Hyclone (Tian Hang bio tech ltd, Zhejiang Lot.No.100419); NaHCO3 (Shanghai hundred million chemical reagent company limited Cat.No.11810-033 Lot.No.1088387 of a specified duration); Trypsin (AMRESCO company lot number: 2010/04); EDTA (AMRESCO company lot number: 2009/10); Penicillin G Sodium Salt (AMRESCO company lot number: 2010242); Streptomycin Sulfate (AMRESCO company lot number: 2010382); Dehydrated alcohol (Nanjing Chemistry Reagent Co., Ltd.'s lot number: 080310182); MTT (Biosharp lot number: 0793); PBS (laboratory autogamy);
1.4 experiment equipments: Lycra inverted microscope (German Leica model: DM1L); Visible-ultraviolet light microwell plate detector (MD company of U.S. model: SPECTRA MAX 190); CO2 incubator (FORMA model: 3111); Super-clean bench (safe and sound Inc. of Su Jing group moulding number: SW-CJ-ZFD); Pure water instrument (Spring company of U.S. model: S/N 020579); Accurate pipettor (French Gilson Inc model: P2); Electronic balance (German Sai Duolisi company limited model: BT323S); Full-automatic high-pressure autoclave (Japanese SANYO company model: MLS-3020); Table electrothermal air dry oven (the accurate experimental facilities company in Shanghai model: DHG9123A); Refrigerator (Siemens Company's model: KG18V21TI); Liquid nitrogen container (CBS model: 2001); Low speed centrifuge (Anting Scientific Instrument Factory, Shanghai's model: KA-1000); 0.2 μm of filter (MILLIPORE model: SLGP033RB); 10cm culture dish (NEST company), 96 well culture plates (NEST company); Cell counting count board; Centrifuge tube, pipet, Tips are some.
2 experimental techniques: 1) U937 cell DMEM+10%FBS in 37 DEG C, 5%CO2 carries out cellar culture (10cm culture dish), when Growth of Cells is to logarithmic (log) phase, collecting cell, discards culture fluid, PBS fine laundering 3 times, add 3ml 0.25% trypsin-0.04%EDTA, after 37 DEG C of digestion 2min, add 5ml complete medium neutralization reaction wherein, proceeded in centrifuge tube after piping and druming cell, the centrifugal 5min of 1000rpm, adjustment concentration of cell suspension 3 × 104/ml.2) enter in 96 well culture plates by cell kind, every hole adds cell suspension 180 μ l, and culture plate puts into cell culture incubator (37 DEG C, 5%CO2) cellar culture.3) according to cell growth status, generally grow to 50%-70%, add unit and positive stomach sheet suspension, continue to cultivate 24h.4) add 20 μ l MTT solution (5mg/ml, i.e. 0.5%MTT) after 24h, continue to cultivate 4h.5) after 4h, buckle method removes supernatant, pats dry gently with absorbent paper, and every hole adds 200 μ l dimethyl sulfoxide, puts low-speed oscillation 10min on shaking table, crystal is fully dissolved.The light absorption value in each hole is measured at enzyme-linked immunosorbent assay instrument 490nm place.6) background (do not add cell, only add culture fluid) is set, control wells (the medicine dissolution medium of cell, same concentrations, culture fluid, MTT, dimethyl sulfoxide) simultaneously, often organizes the multiple hole of setting 6.7) result represents with the suppression ratio of medicine to cell: cell proliferation suppression ratio (%)=(control wells OD value-dosing holes OD value)/control wells OD value × 100%.Experiment repetition 3 times.
3 experimental results: statistical result showed after mtt assay experiment, compare with matched group, when dosage reaches 5mg/ml, to U937 cell inhibitory effect variant (P<0.05), dosage this difference when 10mg/ml has significance (P<0.01), has pole significant difference (P<0.001) when dosage reaches 15-20mg/ml.
Table 1 yuan and positive stomach sheet are to mouse hypertrophy cell cancer P815 cell inhibitory effect influence research (X ± SD)
Note: compare with matched group, * P<0.01; * P<0.001
4 experiment conclusion: first and positive stomach sheet can suppress mouse hypertrophy cell cancer P815 cell proliferation, reduce the Growth of Cells number of mouse hypertrophy cell cancer P815 cell, this effect is dose dependent.
Claims (6)
1. the preparation method of a unit and positive stomach sheet, be made up as crude drug of Radix Et Rhizoma Rhei 300g, Radix Gentianae 300g, Radix Aucklandiae 300g, Rhizoma Corydalis 200g, Herba Menthae 25g, Radix Glycyrrhizae 200g, Flos Caryophylli 20g, sodium bicarbonate 600g, it is characterized in that described method is made up of the following step: get Radix Et Rhizoma Rhei 300g, Radix Gentianae 300g, Radix Aucklandiae 300g, Rhizoma Corydalis 200g, Herba Menthae 25g, Radix Glycyrrhizae 200g, Flos Caryophylli 20g, join CO
2in supercritical extraction device, ethanol is as entrainer, and the percent by volume that entrainer accounts for total extractant is 4-6%, extracting pressure 15-30MPa, temperature 30-50 DEG C, CO
2flow 1-3m1/g crude drug min, extraction time 150-180min, obtains supercritical extract, adds sodium bicarbonate 600g, copolyvidone 20g, 70% ethanol granule, and dry, tabletting, makes 800, the heavy 0.75g of every sheet.
2. unit and the preparation method of positive stomach sheet according to claim 1, is characterized in that described CO
2the percent by volume that supercritical extraction entrainer accounts for total extractant is 5%.
3. unit and the preparation method of positive stomach sheet according to claim 1, is characterized in that described CO
2the extracting pressure 20MPa of supercritical extraction, temperature 40 DEG C, CO
2flow 2ml/g crude drug min, extraction time 160min.
4. unit and positive stomach sheet suppress the application in mouse hypertrophy cell cancer P815 cell proliferation in preparation according to claim 1, it is characterized in that described method is made up of the following step: get Radix Et Rhizoma Rhei 300g, Radix Gentianae 300g, Radix Aucklandiae 300g, Rhizoma Corydalis 200g, Herba Menthae 25g, Radix Glycyrrhizae 200g, Flos Caryophylli 20g, join in CO2 supercritical extraction device, ethanol is as entrainer, the percent by volume that entrainer accounts for total extractant is 4-6%, extracting pressure 15-30MPa, temperature 30-50 DEG C, CO2 flow 1-3m1/g crude drug min, extraction time 150-180min, obtain supercritical extract, add sodium bicarbonate 600g, copolyvidone 20g, 70% ethanol granule, dry, tabletting, make 800, the heavy 0.75g of every sheet.
5. unit and the application of positive stomach sheet in preparation suppression mouse hypertrophy cell cancer P815 cell proliferation according to claim 4, CO described in the preparation method that it is characterized in that YIXUANNING
2the percent by volume that supercritical extraction entrainer accounts for total extractant is 5%.
6. unit and the application of positive stomach sheet in preparation suppression mouse hypertrophy cell cancer P815 cell proliferation according to claim 4, CO described in the preparation method that it is characterized in that YIXUANNING
2the extracting pressure 20MPa of supercritical extraction, temperature 40 DEG C, CO
2flow 2ml/g crude drug min, extraction time 160min.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510283362.0A CN104922227A (en) | 2015-05-28 | 2015-05-28 | Preparation method of Yuanhe stomach tablet and application |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510283362.0A CN104922227A (en) | 2015-05-28 | 2015-05-28 | Preparation method of Yuanhe stomach tablet and application |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN104922227A true CN104922227A (en) | 2015-09-23 |
Family
ID=54109880
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510283362.0A Pending CN104922227A (en) | 2015-05-28 | 2015-05-28 | Preparation method of Yuanhe stomach tablet and application |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN104922227A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106038724A (en) * | 2016-07-11 | 2016-10-26 | 南京多宝生物科技有限公司 | Yuanhe Zheng Wei tablets and preparation method thereof |
| CN107714783A (en) * | 2017-11-07 | 2018-02-23 | 全椒先奇医药科技有限公司 | A kind of first and positive stomach piece |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101104018A (en) * | 2007-02-13 | 2008-01-16 | 通化正和药业有限公司 | Medicinal composition for treating stomach disease and preparation process thereof |
| CN102988667A (en) * | 2012-10-15 | 2013-03-27 | 李正梅 | Preparation method and application of tablet for regulating menstruation and activating blood |
| CN103494885A (en) * | 2013-10-11 | 2014-01-08 | 南京正亮医药科技有限公司 | Preparation method and application of orifice-freeing rhinitis tablets |
| CN103536725A (en) * | 2013-10-31 | 2014-01-29 | 刘美福 | Preparation method and application of priceless health restoring powder |
| CN103721136A (en) * | 2013-11-28 | 2014-04-16 | 淄博齐鼎立专利信息咨询有限公司 | Preparation method and application of pharyngitis tablet |
-
2015
- 2015-05-28 CN CN201510283362.0A patent/CN104922227A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101104018A (en) * | 2007-02-13 | 2008-01-16 | 通化正和药业有限公司 | Medicinal composition for treating stomach disease and preparation process thereof |
| CN102988667A (en) * | 2012-10-15 | 2013-03-27 | 李正梅 | Preparation method and application of tablet for regulating menstruation and activating blood |
| CN103494885A (en) * | 2013-10-11 | 2014-01-08 | 南京正亮医药科技有限公司 | Preparation method and application of orifice-freeing rhinitis tablets |
| CN103536725A (en) * | 2013-10-31 | 2014-01-29 | 刘美福 | Preparation method and application of priceless health restoring powder |
| CN103721136A (en) * | 2013-11-28 | 2014-04-16 | 淄博齐鼎立专利信息咨询有限公司 | Preparation method and application of pharyngitis tablet |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106038724A (en) * | 2016-07-11 | 2016-10-26 | 南京多宝生物科技有限公司 | Yuanhe Zheng Wei tablets and preparation method thereof |
| CN107714783A (en) * | 2017-11-07 | 2018-02-23 | 全椒先奇医药科技有限公司 | A kind of first and positive stomach piece |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103494878A (en) | Preparation method and application of compound danshen tablets | |
| CN103495117B (en) | Preparation method and application of thrombus removing and collaterals dredging tablets | |
| CN103494885B (en) | A kind of preparation method of TONGXIAO BIYAN PIAN and application | |
| CN104922227A (en) | Preparation method of Yuanhe stomach tablet and application | |
| CN104940426A (en) | Preparation method and application of osteoproliferation removal tablets | |
| CN104983691A (en) | Preparation method and application of compound indigowoad root granules | |
| CN103505517B (en) | A kind of preparation method of gastralgia tablet and application | |
| CN103479749B (en) | Preparation method and application of Gegenqinlian tablet | |
| CN103520446B (en) | A kind of preparation method of the Zijin ball that bonesets and application | |
| CN103494915B (en) | Preparation method and application of breast lump dissipating tablet | |
| CN103494930B (en) | A kind of preparation method of FUFANG YUXINGCAO PIAN and application | |
| CN103536691B (en) | A kind of preparation method of Schisandrae Pill and application | |
| CN104887814A (en) | Preparation method and application of Shenqi Wuweizi capsules | |
| CN103721136B (en) | A kind of preparation method of Yanyan slice and application | |
| CN103536654B (en) | A kind of preparation method of Chuan Xiong Tea ball and application | |
| CN103520296B (en) | A kind of preparation method for the treatment of coronary heart disease granule and application | |
| CN103494857B (en) | A kind of preparation method of SHUXIONG PIAN and application | |
| CN103494933B (en) | Preparation method and application of liver protection tablet | |
| CN104922447A (en) | Health improvement capsule preparation method and application | |
| CN103721071B (en) | A kind of preparation method of YULU Baofei bolus and application | |
| CN103494869B (en) | A kind of preparation method of SHILINTONG PIAN and application | |
| CN104840797A (en) | Preparation method and application of Yixuanning capsule | |
| CN104906260A (en) | Preparation method and application of wind-expelling and itching-relieving granule | |
| CN103520294A (en) | Preparation method for Sanhuang tablet | |
| CN103623212B (en) | A kind of preparation method and application consolidating ball |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20150923 |