CN104887814A - Preparation method and application of Shenqi Wuweizi capsules - Google Patents
Preparation method and application of Shenqi Wuweizi capsules Download PDFInfo
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- CN104887814A CN104887814A CN201510346715.7A CN201510346715A CN104887814A CN 104887814 A CN104887814 A CN 104887814A CN 201510346715 A CN201510346715 A CN 201510346715A CN 104887814 A CN104887814 A CN 104887814A
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Abstract
The invention belongs to the field of Chinese herba preparation, and particularly provides a preparation method of Shenqi Wuweizi capsules. The Shenqi Wuweizi capsules are prepared by the following bulk drugs: 180g of kadsura longepedunculata, 60g of roots of hairy asiabell, 120g of astragalus membranaceus and 30g of semen ziziphi spinosae in a supercritical fluid extraction; the content is greatly improved; and the using level of the bulk drugs is reduced. The invention also provides an application of the Shenqi Wuweizi capsules on preparation of medicines for restraining proliferation of epithelial cells D407 of human retinochrome.
Description
Technical field
The present invention relates to technical field of traditional Chinese medicine preparation, be specifically related to preparation method and the application of a seed ginseng stilbene Fructus Schisandrae Chinensis capsule.
Background technology
Ginseng stilbene Fructus Schisandrae Chinensis capsule is recorded in national drug food Surveillance Authority standard, and prescription is Fructus Schisandrae Sphenantherae 180g, Radix Codonopsis 60g, Radix Astragali 120g, Semen Ziziphi Spinosae (parched) 30g, and method for making is above four tastes, and Radix Codonopsis and Fructus Schisandrae Sphenantherae 60g are ground into fine powder; The Radix Astragali, Semen Ziziphi Spinosae (parched) and remaining Fructus Schisandrae Sphenantherae use 45% ethanol, 70% ethanol and 60% ethanol as solvent respectively, flood and carry out percolation after 24 hours, collect percolate, merge after reclaiming ethanol, be concentrated into appropriate, add above-mentioned fine powder and appropriate adjuvant, mixing, makes granule, encapsulating capsule, obtain 1000, every 0.3g.There is invigorating the spleen and benefiting QI, mind tranquilizing and the heart calming function.For insufficiency of vital energy and blood, the insomnia caused by deficiency of both the heart and spleen, dreaminess, forgetful, weak, cardiopalmus, breathe hard, spontaneous perspiration.
In prior art, not yet there is ginseng stilbene Fructus Schisandrae Chinensis capsule extracting the report adopting supercritical technology in preparation, and adopt the methods such as alcohol extraction, technique is coarse, complicated, backward, and impurity is many, causes patient's consumption excessive, be inconvenient to take, had a strong impact on this product and applied clinically.
In prior art, ginseng stilbene Fructus Schisandrae Chinensis capsule every 0.3g, oral, one time 2 ~ 3,3 times on the one, adopt ginseng stilbene Fructus Schisandrae Chinensis capsule every 0.3g of being prepared into of the present invention, but the medical material amount contained is more than originally, therefore only need 1-2 grain at every turn, within 1st, take 3 times, under the condition with more active component, greatly reduce dose.
Summary of the invention
Goal of the invention: in order to solve the problem, the object of the present invention is to provide the preparation method of a seed ginseng stilbene Fructus Schisandrae Chinensis capsule.
Another object of the present invention is to provide a seed ginseng stilbene Fructus Schisandrae Chinensis capsule to suppress the application in Human RPE Cells in Vitro D407 hyperproliferation agent in preparation.
Technical scheme: the object of the invention is by following scheme realize:
The preparation method of one seed ginseng stilbene Fructus Schisandrae Chinensis capsule, by Fructus Schisandrae Sphenantherae 180g, Radix Codonopsis 60g, Radix Astragali 120g, Semen Ziziphi Spinosae (parched) 30g makes as crude drug, described method is made up of the following step: get Fructus Schisandrae Sphenantherae 180g, Radix Codonopsis 60g, Radix Astragali 120g, Semen Ziziphi Spinosae (parched) 30g medical material joins in CO2 supercritical extraction device, ethanol is as entrainer, the percent by volume that entrainer accounts for total extractant is 4-6%, extracting pressure 15-30MPa, temperature 30-50 DEG C, CO2 flow 1-3m1/g crude drug min, extraction time 150-180min, obtain supercritical extract, pulverize, add starch 20g, 70% ethanol granule, dry, encapsulated, make 800, every heavy 0.3g.
The preparation method of described ginseng stilbene Fructus Schisandrae Chinensis capsule, the percent by volume that described CO2 supercritical extraction entrainer accounts for total extractant is 5%.
The preparation method of described ginseng stilbene Fructus Schisandrae Chinensis capsule, the extracting pressure 20MPa of described CO2 supercritical extraction, temperature 40 DEG C, CO2 flow 2ml/g crude drug min, extraction time 160min.
Described ginseng stilbene Fructus Schisandrae Chinensis capsule suppresses the application in Human RPE Cells in Vitro D407 hyperproliferation agent in preparation, ginseng stilbene Fructus Schisandrae Chinensis capsule is by Fructus Schisandrae Sphenantherae 180g, Radix Codonopsis 60g, Radix Astragali 120g, Semen Ziziphi Spinosae (parched) 30g makes as crude drug, preparation method is: get Fructus Schisandrae Sphenantherae 180g, Radix Codonopsis 60g, Radix Astragali 120g, Semen Ziziphi Spinosae (parched) 30g joins in CO2 supercritical extraction device, ethanol is as entrainer, the percent by volume that entrainer accounts for total extractant is 4-6%, extracting pressure 15-30MPa, temperature 30-50 DEG C, CO2 flow 1-3m1/g crude drug min, extraction time 150-180min, obtain supercritical extract, pulverize, add starch 20g, 70% ethanol granule, dry, encapsulated, make 800, every heavy 0.3g.
Described ginseng stilbene Fructus Schisandrae Chinensis capsule suppresses the application in Human RPE Cells in Vitro D407 hyperproliferation agent in preparation, and described in the preparation method that it is characterized in that joining stilbene Fructus Schisandrae Chinensis capsule, CO2 supercritical extraction entrainer accounts for the percent by volume of total extractant is 5%.
Described ginseng stilbene Fructus Schisandrae Chinensis capsule suppresses the application in Human RPE Cells in Vitro D407 hyperproliferation agent in preparation, the extracting pressure 20MPa of CO2 supercritical extraction described in the preparation method that it is characterized in that joining stilbene Fructus Schisandrae Chinensis capsule, temperature 40 DEG C, CO2 flow 2ml/g crude drug min, extraction time 160min.
Detailed description of the invention
Form by the following examples, foregoing of the present invention is described in further detail again, but this should be interpreted as that the scope of the above-mentioned theme of the present invention is only limitted to following example, all technology realized based on foregoing of the present invention all belong to scope of the present invention.
Embodiment 1: get Fructus Schisandrae Sphenantherae 180g, Radix Codonopsis 60g, Radix Astragali 120g, Semen Ziziphi Spinosae (parched) 30g, join in CO2 supercritical extraction device, ethanol is as entrainer, the percent by volume that entrainer accounts for total extractant is 4%, extracting pressure 30MPa, temperature 30 DEG C, CO2 flow 1m1/g crude drug min, extraction time 180min, obtains supercritical extract, pulverize, add starch 20g, 70% ethanol granule, dry, encapsulated, make 800, every heavy 0.3g.
Embodiment 2: get Fructus Schisandrae Sphenantherae 180g, Radix Codonopsis 60g, Radix Astragali 120g, Semen Ziziphi Spinosae (parched) 30g, join in CO2 supercritical extraction device, ethanol is as entrainer, the percent by volume that entrainer accounts for total extractant is 6%, extracting pressure 15MPa, temperature 50 C, CO2 flow 1m1/g crude drug min, extraction time 180min, obtains supercritical extract, pulverize, add starch 20g, 70% ethanol granule, dry, encapsulated, make 800, every heavy 0.3g.
Embodiment 3: get Fructus Schisandrae Sphenantherae 180g, Radix Codonopsis 60g, Radix Astragali 120g, Semen Ziziphi Spinosae (parched) 30g, join in CO2 supercritical extraction device, ethanol is as entrainer, the percent by volume that entrainer accounts for total extractant is 5%, extracting pressure 20MPa, temperature 40 DEG C, CO2 flow 2m1/g crude drug min, extraction time 160min, obtains supercritical extract, pulverize, add starch 20g, 70% ethanol granule, dry, encapsulated, make 800, every heavy 0.3g.
Embodiment 4: ginseng stilbene Fructus Schisandrae Chinensis capsule suppresses the experimentation data of Human RPE Cells in Vitro D407 propagation
1 experiment material
1.1 experiment cell strains: Human RPE Cells in Vitro D407, Nanjing Medical University's laboratory cell storehouse, DMEM+10%FBS cellar culture.
1.2 Experimental agents: drugs: the present invention joins stilbene Fructus Schisandrae Chinensis capsule: prepare by embodiment 3 method.Medicinal liquid liquid storage: take 100mg and join stilbene Fructus Schisandrae Chinensis capsule 's content, be suspended in 5ml dehydrated alcohol, 0.2 μm of frit, 500 μ l doff pipe subpackages ,-20 DEG C of storage, 0.2 μm of frit dehydrated alcohol prepares against the use of matched group simultaneously.
1.3 experiment reagents: DMEM (GIBCO company Cat.No.12100-061Lot.No.758137); Hyclone (Tian Hang bio tech ltd, Zhejiang Lot.No.100419); NaHCO3 (Shanghai hundred million chemical reagent company limited Cat.No.11810-033Lot.No.1088387 of a specified duration); Trypsin (AMRESCO company lot number: 2010/04); EDTA (AMRESCO company lot number: 2009/10); Penicillin G Sodium Salt (AMRESCO company lot number: 2010242); Streptomycin Sulfate (AMRESCO company lot number: 2010382); Dehydrated alcohol (Nanjing Chemistry Reagent Co., Ltd.'s lot number: 080310182); MTT (Biosharp lot number: 0793); PBS (laboratory autogamy);
1.4 experiment equipments: Lycra inverted microscope (German Leica model: DM1L); Visible-ultraviolet light microwell plate detector (MD company of U.S. model: SPECTRA MAX 190); CO2 incubator (FORMA model: 3111); Super-clean bench (safe and sound Inc. of Su Jing group moulding number: SW-CJ-ZFD); Pure water instrument (Spring company of U.S. model: S/N 020579); Accurate pipettor (French Gilson Inc model: P2); Electronic balance (German Sai Duolisi company limited model: BT323S); Full-automatic high-pressure autoclave (Japanese SANYO company model: MLS-3020); Table electrothermal air dry oven (the accurate experimental facilities company in Shanghai model: DHG9123A); Refrigerator (Siemens Company's model: KG18V21TI); Liquid nitrogen container (CBS model: 2001); Low speed centrifuge (Anting Scientific Instrument Factory, Shanghai's model: KA-1000); 0.2 μm of filter (MILLIPORE model: SLGP033RB); 10cm culture dish (NEST company), 96 well culture plates (NEST company); Cell counting count board; Centrifuge tube, pipet, Tips are some.
2 experimental techniques: 1) D407 cell DMEM+10%FBS in 37 DEG C, 5%CO2 carries out cellar culture (10cm culture dish), when Growth of Cells is to logarithmic (log) phase, collecting cell, discards culture fluid, PBS fine laundering 3 times, add 3ml 0.25% trypsin-0.04%EDTA, after 37 DEG C of digestion 2min, add 5ml complete medium neutralization reaction wherein, proceeded in centrifuge tube after piping and druming cell, the centrifugal 5min of 1000rpm, adjustment concentration of cell suspension 3 × 104/ml.2) enter in 96 well culture plates by cell kind, every hole adds cell suspension 180 μ l, and culture plate puts into cell culture incubator (37 DEG C, 5%CO2) cellar culture.3) according to cell growth status, generally grow to 50%-70%, add ginseng stilbene Fructus Schisandrae Chinensis capsule solution, continue to cultivate 24h.4) add 20 μ l MTT solution (5mg/ml, i.e. 0.5%MTT) after 24h, continue to cultivate 4h.5) after 4h, buckle method removes supernatant, pats dry gently with absorbent paper, and every hole adds 200 μ l dimethyl sulfoxide, puts low-speed oscillation 10min on shaking table, crystal is fully dissolved.The light absorption value in each hole is measured at enzyme-linked immunosorbent assay instrument 490nm place.6) background (do not add cell, only add culture fluid) is set, control wells (the medicine dissolution medium of cell, same concentrations, culture fluid, MTT, dimethyl sulfoxide) simultaneously, often organizes the multiple hole of setting 6.7) result represents with the suppression ratio of medicine to cell: cell proliferation suppression ratio (%)=(control wells OD value-dosing holes OD value)/control wells OD value × 100%.Experiment repetition 3 times.
3 experimental results: statistical result showed after mtt assay experiment, compare with matched group, when dosage reaches 5mg/ml, to D407 cell inhibitory effect variant (P<0.05), dosage this difference when 10mg/ml has significance (P<0.01), has pole significant difference (P<0.001) when dosage reaches 15-20 mg/ml.
Table 1 joins stilbene Fructus Schisandrae Chinensis capsule to Human RPE Cells in Vitro D407 Proliferation Ability influence research (X ± SD)
Note: compare with matched group, * P<0.01; * P<0.001
4 experiment conclusion: ginseng stilbene Fructus Schisandrae Chinensis capsule can suppress Human RPE Cells in Vitro D407 to breed, and reduce the Growth of Cells number of Human RPE Cells in Vitro D407, this effect is dose dependent.
Claims (6)
1. the preparation method of a seed ginseng stilbene Fructus Schisandrae Chinensis capsule, be made up as crude drug of Fructus Schisandrae Sphenantherae 180g, Radix Codonopsis 60g, Radix Astragali 120g, Semen Ziziphi Spinosae (parched) 30g, it is characterized in that described method is made up of the following step: get Fructus Schisandrae Sphenantherae 180g, Radix Codonopsis 60g, Radix Astragali 120g, Semen Ziziphi Spinosae (parched) 30g medical material join CO
2in supercritical extraction device, ethanol is as entrainer, and the percent by volume that entrainer accounts for total extractant is 4-6%, extracting pressure 15-30MPa, temperature 30-50 DEG C, CO
2flow 1-3m1/g crude drug min, extraction time 150-180min, obtains supercritical extract, pulverizes, adds starch 20g, 70% ethanol granule, dry, encapsulated, makes 800, every heavy 0.3g.
2. join the preparation method of stilbene Fructus Schisandrae Chinensis capsule according to claim 1, it is characterized in that described CO
2the percent by volume that supercritical extraction entrainer accounts for total extractant is 5%.
3. join the preparation method of stilbene Fructus Schisandrae Chinensis capsule according to claim 1, it is characterized in that described CO
2the extracting pressure 20MPa of supercritical extraction, temperature 40 DEG C, CO
2flow 2ml/g crude drug min, extraction time 160min.
4. join the application of stilbene Fructus Schisandrae Chinensis capsule in preparation suppression Human RPE Cells in Vitro D407 hyperproliferation agent according to claim 1, it is characterized in that ginseng stilbene Fructus Schisandrae Chinensis capsule is made up as crude drug of Fructus Schisandrae Sphenantherae 180g, Radix Codonopsis 60g, Radix Astragali 120g, Semen Ziziphi Spinosae (parched) 30g, preparation method is: get Fructus Schisandrae Sphenantherae 180g, Radix Codonopsis 60g, Radix Astragali 120g, Semen Ziziphi Spinosae (parched) 30g join CO
2in supercritical extraction device, ethanol is as entrainer, and the percent by volume that entrainer accounts for total extractant is 4-6%, extracting pressure 15-30MPa, temperature 30-50 DEG C, CO
2flow 1-3m1/g crude drug min, extraction time 150-180min, obtains supercritical extract, adds starch 20g, 70% ethanol granule, dry, encapsulated, makes 800, every heavy 0.3g.
5. join stilbene Fructus Schisandrae Chinensis capsule suppresses in Human RPE Cells in Vitro D407 hyperproliferation agent application in preparation according to claim 4, CO described in the preparation method that it is characterized in that joining stilbene Fructus Schisandrae Chinensis capsule
2the percent by volume that supercritical extraction entrainer accounts for total extractant is 5%.
6. join stilbene Fructus Schisandrae Chinensis capsule suppresses in Human RPE Cells in Vitro D407 hyperproliferation agent application in preparation according to claim 4, CO described in the preparation method that it is characterized in that joining stilbene Fructus Schisandrae Chinensis capsule
2the extracting pressure 20MPa of supercritical extraction, temperature 40 DEG C, CO
2flow 2ml/g crude drug min, extraction time 160min.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106237055A (en) * | 2016-08-30 | 2016-12-21 | 山东百维药业有限公司 | A kind of its preparation method of the Chinese medicine composition for treating acne |
| CN106310072A (en) * | 2016-08-30 | 2017-01-11 | 山东百维药业有限公司 | Shenqiwuweizi capsules and preparation method thereof |
-
2015
- 2015-06-20 CN CN201510346715.7A patent/CN104887814A/en active Pending
Non-Patent Citations (2)
| Title |
|---|
| 国家药典委员会: "《中华人民共和国药典》", 31 December 2010 * |
| 祝洪艳等: "参芪五味子颗粒协同戊巴比妥钠对小鼠睡眠及免疫器官指数的影响", 《上海中医药杂志》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106237055A (en) * | 2016-08-30 | 2016-12-21 | 山东百维药业有限公司 | A kind of its preparation method of the Chinese medicine composition for treating acne |
| CN106310072A (en) * | 2016-08-30 | 2017-01-11 | 山东百维药业有限公司 | Shenqiwuweizi capsules and preparation method thereof |
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Application publication date: 20150909 |