CN103494898A - Preparation method and application of refined tablet for treating coronary disease - Google Patents
Preparation method and application of refined tablet for treating coronary disease Download PDFInfo
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- CN103494898A CN103494898A CN201310467583.4A CN201310467583A CN103494898A CN 103494898 A CN103494898 A CN 103494898A CN 201310467583 A CN201310467583 A CN 201310467583A CN 103494898 A CN103494898 A CN 103494898A
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Abstract
The invention provides a preparation method of a refined tablet for treating a coronary disease. The refined tablet for treating coronary disease is prepared by carrying out supercritical fluid extraction on the following raw herbal materials: 375g of root of red-rooted salvia, 187.5g of radix paeoniae rubra, 187.5g of ligusticum wallichii, 187.5g of carthamus tinctorious and 125g lignum acronychiae, so that the content is greatly increased, and the dose is reduced. The invention also provides application of the refined tablet for treating the coronary disease in the preparation of a medicine for inhibiting the proliferation of a mouse myeloma cell SP2/0.
Description
Technical field
The present invention relates to the Chinese medicine preparation technical field, be specifically related to a kind of preparation method and application of Jingzhi Guanxin tablet.
Background technology
Jingzhi Guanxin tablet is recorded in pharmacopeia, writes out a prescription as Radix Salviae Miltiorrhizae 375g, Radix Paeoniae Rubra 187.5g, Rhizoma Chuanxiong 187.5g, Flos Carthami 187.5g, Lignum Dalbergiae Odoriferae 125g, and the above five tastes, Lignum Dalbergiae Odoriferae extracts volatile oil, and the another device of the aqueous solution after distillation is collected; All the other Radix Paeoniae Rubra etc. four are distinguished the flavor of with 85% alcohol heating reflux secondary, 3 hours for the first time, 2 hours for the second time, filter, merging filtrate, reclaim ethanol, with above-mentioned aqueous solution, merge, being evaporated to relative density is 1.35～1.40(50 ℃), add right amount of auxiliary materials, granulation, drying, add volatile oil of Lignum Dalbergiae Odoriferae, mix, be pressed into 1000, sugar coating, obtain, can blood circulation promoting and blood stasis dispelling.For the coronary heart disease of stagnation of heart-blood, angina pectoris.
In prior art, not yet there is Jingzhi Guanxin tablet adopting the report of supercritical technology aspect the extraction preparation, and the method that adopts decocting to boil, technique is coarse, backward, and impurity is many, causes patient's consumption excessive, is inconvenient to take, and has had a strong impact on this product and has applied clinically.
Summary of the invention
Goal of the invention: in order to address the above problem, the object of the present invention is to provide a kind of preparation method of Jingzhi Guanxin tablet.
Another object of the present invention is to provide a kind of Jingzhi Guanxin tablet to suppress the application in mouse myeloma SP2/0 cell proliferation medicine in preparation.
Technical scheme: the objective of the invention is to realize by following scheme:
A kind of preparation method of Jingzhi Guanxin tablet, by Radix Salviae Miltiorrhizae 375g, Radix Paeoniae Rubra 187.5g, Rhizoma Chuanxiong 187.5g, Flos Carthami 187.5g, Lignum Dalbergiae Odoriferae 125g, as crude drug, made, described method is comprised of the following step: get Radix Salviae Miltiorrhizae 375g, Radix Paeoniae Rubra 187.5g, Rhizoma Chuanxiong 187.5g, Flos Carthami 187.5g, Lignum Dalbergiae Odoriferae 125g, join in CO2 supercritical extraction device, ethanol is as entrainer, and the percent by volume that entrainer accounts for total extractant is 4-6%, extracting pressure 15-30MPa, temperature 30-50 ℃, CO
2flow 1-3m1/g crude drug min, extraction time 150-180min, obtain supercritical extract, adds starch, 70% ethanol granule processed, drying, tabletting, make 500, every heavy 0.5g.
The preparation method of above-mentioned a kind of Jingzhi Guanxin tablet, described CO
2the percent by volume that the supercritical extraction entrainer accounts for total extractant is 5%.
The preparation method of above-mentioned a kind of Jingzhi Guanxin tablet, described CO
2the extracting pressure 20MPa of supercritical extraction, 40 ℃ of temperature, CO
2flow 2ml/g crude drug min, extraction time 160min.
Above-mentioned a kind of Jingzhi Guanxin tablet suppresses the application in mouse myeloma SP2/0 cell proliferation medicine in preparation, Jingzhi Guanxin tablet by Plumbum preparatium join 375g, Radix Paeoniae Rubra 187.5g, Rhizoma Chuanxiong 187.5g, Flos Carthami 187.5g, Lignum Dalbergiae Odoriferae 125g makes as crude drug, preparation method is comprised of the following step: get Radix Salviae Miltiorrhizae 375g, Radix Paeoniae Rubra 187.5g, Rhizoma Chuanxiong 187.5g, Flos Carthami 187.5g, Lignum Dalbergiae Odoriferae 125g, join CO
2in the supercritical extraction device, ethanol is as entrainer, and the percent by volume that entrainer accounts for total extractant is 4-6%, extracting pressure 15-30MPa, temperature 30-50 ℃, CO
2flow 1-3m1/g crude drug min, extraction time 150-180min, obtain supercritical extract, adds starch, 70% ethanol granule processed, drying, tabletting, make 500, every heavy 0.5g.
Above-mentioned a kind of Jingzhi Guanxin tablet suppresses the application in mouse myeloma SP2/0 cell proliferation medicine, CO described in Jingzhi Guanxin tablet in preparation
2the percent by volume that the supercritical extraction entrainer accounts for total extractant is 5%.
Above-mentioned a kind of Jingzhi Guanxin tablet suppresses the application in mouse myeloma SP2/0 cell proliferation medicine, CO described in Jingzhi Guanxin tablet in preparation
2the extracting pressure 20MPa of supercritical extraction, 40 ℃ of temperature, CO
2flow 2ml/g crude drug min, extraction time 160min.
In prior art, every 0.5g of Jingzhi Guanxin tablet, each 4,2-3 time on the one, the every 0.5g of Jingzhi Guanxin tablet that adopts the present invention to be prepared into, but the medical material amount contained is original 2 times, therefore only needs 2 at every turn, within 1st, take 2 times, greatly reduced dose having under the condition of more active component.
The specific embodiment
Form by the following examples, foregoing of the present invention is described in further detail again, but this should be interpreted as to the scope of the above-mentioned theme of the present invention only limits to following example, all technology realized based on foregoing of the present invention all belong to scope of the present invention.
Embodiment 1
Get Radix Salviae Miltiorrhizae 375g, Radix Paeoniae Rubra 187.5g, Rhizoma Chuanxiong 187.5g, Flos Carthami 187.5g, Lignum Dalbergiae Odoriferae 125g, join in CO2 supercritical extraction device, ethanol is as entrainer, and the percent by volume that entrainer accounts for total extractant is 4%, extracting pressure 15MPa, 30 ℃ of temperature, CO
2flow 1m1/g crude drug min, extraction time 150min, obtain supercritical extract, adds starch, 70% ethanol granule processed, drying, tabletting, make 500, every heavy 0.5g.
Embodiment 2
Get Radix Salviae Miltiorrhizae 375g, Radix Paeoniae Rubra 187.5g, Rhizoma Chuanxiong 187.5g, Flos Carthami 187.5g, Lignum Dalbergiae Odoriferae 125g joins CO
2in the supercritical extraction device, ethanol is as entrainer, and the percent by volume that entrainer accounts for total extractant is 6%, extracting pressure 30MPa, temperature 50 C, CO
2flow 3m1/g crude drug min, extraction time 180min, obtain supercritical extract, adds starch, 70% ethanol granule processed, drying, tabletting, make 500, every heavy 0.5g.
Embodiment 3
Get Radix Salviae Miltiorrhizae 375g, Radix Paeoniae Rubra 187.5g, Rhizoma Chuanxiong 187.5g, Flos Carthami 187.5g, Lignum Dalbergiae Odoriferae 125g joins CO
2in the supercritical extraction device, ethanol is as entrainer, and the percent by volume that entrainer accounts for total extractant is 5%, extracting pressure 20MPa, 40 ℃ of temperature, CO
2flow 2m1/g crude drug min, extraction time 160min, obtain supercritical extract, adds starch, 70% ethanol granule processed, drying, tabletting, make 500, every heavy 0.5g.
Embodiment 4: Jingzhi Guanxin tablet suppresses the experimentation data of SP2/0 cell proliferation
1 experiment material
1.1 experiment cell strain
Mouse myeloma SP2/0 cell, Nanjing Zheng Liang Pharmaceutical Technology Co., Ltd laboratory cell bank, DMEM+10%FBS cellar culture.
1.2 Experimental agents
Drugs: Jingzhi Guanxin tablet of the present invention: press embodiment 3 method preparations.
The medicinal liquid liquid storage: take the 100mg Jingzhi Guanxin tablet, be dissolved in the 5ml dehydrated alcohol, 0.2 μ m filter filters, 500 μ ldoff pipe packing, and-20 ℃ of storages, 0.2 μ m filter filters the use of dehydrated alcohol in order to matched group simultaneously.
1.3 experiment reagent
The Cat.No.12100-061Lot.No.758137 of DMEM(GIBCO company); Hyclone (Hangzhoupro, sky, Zhejiang bio tech ltd Lot.No.100419); NaHCO3(Shanghai hundred million chemical reagent company limited Cat.No.11810-033Lot.No.1088387 of a specified duration); Trypsin(AMRESCO company lot number: 2010/04); EDTA(AMRESCO company lot number: 2009/10); Penicillin G Sodium Salt(AMRESCO company lot number: 2010242); Streptomycin Sulfate(AMRESCO company lot number: 2010382); Dehydrated alcohol (Nanjing Chemistry Reagent Co., Ltd.'s lot number: 080310182); MTT (Biosharp lot number: 0793); The autogamy of PBS(laboratory);
1.4 experiment equipment
Lycra inverted microscope (German Leica model: DM1L); Visible-ultraviolet light microwell plate detector (U.S. MD company model: SPECTRA MAX190); CO2 incubator (FORMA model: 3111); (safe and sound company of Su Jing group manufactures model to super-clean bench: SW-CJ-ZFD); Pure water instrument (U.S. Spring company model: S/N020579); Accurate pipettor (French Gilson Inc model: P2); Electronic balance (German Sai Duolisi company limited model: BT323S); Full-automatic high-pressure autoclave (Japanese SANYO company model: MLS-3020); Table electrothermal air dry oven (Shanghai accurate experimental facilities company model: DHG9123A); Refrigerator (Siemens Company's model: KG18V21TI); Liquid nitrogen container (CBS model: 2001); Low speed centrifuge (Anting Scientific Instrument Factory, Shanghai's model: KA-1000); 0.2 μ m filter (MILLIPORE model: SLGP033RB); 10cm culture dish (NEST company), 96 well culture plates (NEST company); Cell counting count board; Centrifuge tube, pipet, Tips are some.
2 experimental techniques
1) the SP2/0 cell carries out cellar culture (10cm culture dish) with DMEM+10%FBS in 37 ℃, 5%CO2, when Growth of Cells during to logarithmic (log) phase, collecting cell, discard culture fluid, PBS fine laundering 3 times, add 3ml0.25% trypsin-0.04%EDTA, after 37 ℃ of digestion 2min, add wherein 5ml complete medium neutralization reaction, after the piping and druming cell, it is proceeded in centrifuge tube, the centrifugal 5min of 1000rpm, adjust 3 * 104/ml of concentration of cell suspension.
2) the cell kind is entered in 96 well culture plates, every hole adds cell suspension 180 μ l, culture plate put into cell culture incubator (37 ℃, 5%CO2) cellar culture.
3) according to the Growth of Cells situation, generally grow to 50%-70%, add Jingzhi Guanxin tablet solution, continue to cultivate 24h.
4) add 20 μ l MTT solution (5mg/ml, i.e. 0.5%MTT) after 24h, continue to cultivate 4h.
5) after 4h, the buckle method is removed supernatant, with absorbent paper, pats dry gently, and every hole adds 200 μ l dimethyl sulfoxide, puts low-speed oscillation 10min on shaking table, and crystal is fully dissolved.Measure the light absorption value in each hole at enzyme-linked immunosorbent assay instrument 490nm place.
6) background (do not add cell, only add culture fluid) is set simultaneously, control wells (the medicine dissolution medium of cell, same concentrations, culture fluid, MTT, dimethyl sulfoxide), set 6 multiple holes for every group.
7) with medicine, the suppression ratio to cell means result:
Cell increment suppression ratio (%)=(control wells OD value-dosing holes OD value)/control wells OD value * 100%.Experiment repeats 3 times.
3 statistical dispositions
Adopt correlation analysis and Student t check in Microsoft Excel2003 software, data mean with mean ± S.D..
4 experimental results
Statistical result showed after the mtt assay experiment, with matched group, compare, when dosage reaches 5mg/ml, to SP2/0 cell inhibitory effect variant (P<0.05), dosage this difference when 10mg/ml has significance (P<0.01), and utmost point significant difference (P<0.001) is arranged when dosage reaches 15-20mg/ml.
Table 1 Jingzhi Guanxin tablet affects and grinds the SP2/0 cell inhibitory effect
Annotate: compare * P<0.01 with matched group; * P<0.001
5 experiment conclusion
Jingzhi Guanxin tablet can suppress the SP2/0 cell proliferation, reduces the Growth of Cells number of SP2/0 cell, and this effect is dose dependent.
Claims (6)
1. the preparation method of a Jingzhi Guanxin tablet, by Plumbum preparatium ginseng 375g, Radix Paeoniae Rubra 187.5g, Rhizoma Chuanxiong 187.5g, Flos Carthami 187.5g, Lignum Dalbergiae Odoriferae 125g, as crude drug, made, it is characterized in that described method is comprised of the following step: get Radix Salviae Miltiorrhizae 375g, Radix Paeoniae Rubra 187.5g, Rhizoma Chuanxiong 187.5g, Flos Carthami 187.5g, Lignum Dalbergiae Odoriferae 125g, join CO
2in the supercritical extraction device, ethanol is as entrainer, and the percent by volume that entrainer accounts for total extractant is 4-6%, extracting pressure 15-30MPa, temperature 30-50 ℃, CO
2flow 1-3m1/g crude drug min, extraction time 150-180min, obtain supercritical extract, adds starch, 70% ethanol granule processed, drying, tabletting, make 500, every heavy 0.5g.
2. a kind of preparation method of Jingzhi Guanxin tablet according to claim 1, is characterized in that described CO
2the percent by volume that the supercritical extraction entrainer accounts for total extractant is 5%.
3. a kind of preparation method of Jingzhi Guanxin tablet according to claim 1, is characterized in that described CO
2the extracting pressure 20MPa of supercritical extraction, 40 ℃ of temperature, CO
2flow 2ml/g crude drug min, extraction time 160min.
4. a kind of Jingzhi Guanxin tablet suppresses the application in mouse myeloma SP2/0 cell proliferation medicine in preparation according to claim 1, it is characterized in that Jingzhi Guanxin tablet joins 375g by Plumbum preparatium, Radix Paeoniae Rubra 187.5g, Rhizoma Chuanxiong 187.5g, Flos Carthami 187.5g, Lignum Dalbergiae Odoriferae 125g makes as crude drug, preparation method is comprised of the following step: get Radix Salviae Miltiorrhizae 375g, Radix Paeoniae Rubra 187.5g, Rhizoma Chuanxiong 187.5g, Flos Carthami 187.5g, Lignum Dalbergiae Odoriferae 125g, join in CO2 supercritical extraction device, ethanol is as entrainer, the percent by volume that entrainer accounts for total extractant is 4-6%, extracting pressure 15-30MPa, temperature 30-50 ℃, CO2 flow 1-3m1/g crude drug min, extraction time 150-180min, obtain supercritical extract, add starch, 70% ethanol granule processed, dry, tabletting, make 500, every heavy 0.5g.
5. a kind of Jingzhi Guanxin tablet suppresses the application in mouse myeloma SP2/0 cell proliferation medicine in preparation according to claim 4, it is characterized in that CO described in Jingzhi Guanxin tablet
2the percent by volume that the supercritical extraction entrainer accounts for total extractant is 5%.
6. a kind of Jingzhi Guanxin tablet suppresses the application in mouse myeloma SP2/0 cell proliferation medicine in preparation according to claim 4, it is characterized in that CO described in Jingzhi Guanxin tablet
2the extracting pressure 20MPa of supercritical extraction, 40 ℃ of temperature, CO
2flow 2ml/g crude drug min, extraction time 160min.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103800545A (en) * | 2014-03-14 | 2014-05-21 | 滕邵玲 | Preparation and application of Shuangyang pharyngitis tablets |
| CN104116797A (en) * | 2014-08-19 | 2014-10-29 | 黑龙江江恒医药科技有限公司 | Refined Guanxin tablet and preparation method thereof |
| CN105738499A (en) * | 2016-01-31 | 2016-07-06 | 张春辉 | Method for constructing refined coronary tablet specific chromatogram and measuring six components |
-
2013
- 2013-10-09 CN CN201310467583.4A patent/CN103494898A/en active Pending
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| 古爱虎,汪兴洪: "丹参酮ⅡA 对小鼠骨髓瘤细胞SP2 /0体外增殖与凋亡的影响", 《癌症进展杂志》 * |
| 国家药典委员会: "《中华人民共和国药典2005版一部》", 31 January 2005, 北京：化学工业出版社 * |
| 王承学,刘锐: "超临界CO2萃取丹参酮IIA提取工艺", 《长春工业大学学报( 自然科学版)》 * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103800545A (en) * | 2014-03-14 | 2014-05-21 | 滕邵玲 | Preparation and application of Shuangyang pharyngitis tablets |
| CN104116797A (en) * | 2014-08-19 | 2014-10-29 | 黑龙江江恒医药科技有限公司 | Refined Guanxin tablet and preparation method thereof |
| CN104116797B (en) * | 2014-08-19 | 2016-09-28 | 黑龙江江恒医药科技有限公司 | A kind of Jingzhi Guanxin tablet and preparation method thereof |
| CN105738499A (en) * | 2016-01-31 | 2016-07-06 | 张春辉 | Method for constructing refined coronary tablet specific chromatogram and measuring six components |
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Application publication date: 20140108 |