CN102068684A - Perfusate and preparation method thereof for treating cow recessive mastitis - Google Patents
Perfusate and preparation method thereof for treating cow recessive mastitis Download PDFInfo
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- CN102068684A CN102068684A CN 201110022938 CN201110022938A CN102068684A CN 102068684 A CN102068684 A CN 102068684A CN 201110022938 CN201110022938 CN 201110022938 CN 201110022938 A CN201110022938 A CN 201110022938A CN 102068684 A CN102068684 A CN 102068684A
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Abstract
The invention relates to perfusate for treating cow recessive mastitis, which mainly comprises the raw materials with the following ratios: 4000-5000g of radix astragali, 4000-5000g of herba epimedii, 300-500g of levamisole hydrochloride, 300-400g of antibacterial pepetide, 2000-3000ml of glycerol, 400-500g of gelatine, and 50-100g of sodium benzoate, wherein pH of the perfusate is 5.5-7.5. The perfusate is used for treating cow recessive mastitis with determine curative effect, drug concentration for local application is high, absorption ratio is high, the direction is high, drug application is convenient and safe, thereby the goal of fast treating cow recessive mastitis is achieved, and the perfusate is compound perfusion preparation combining antibiosis, antiphlogosis and improving immunity of the organism.
Description
Technical field
The invention belongs to medical technical field, be specifically related to a kind of infusion liquid that is used for the treatment of the stealthy mastitis of milch cow and preparation method thereof.
Background technology
Stealthy mastitis is one of modal cow disease, the breast inflammation performance of its no clinical symptoms, and promptly sick cow breast and milk all do not have macroscopic variation, are also referred to as subclinical mastitis.Because breast and the no abnormal phenomenon of milk, often ignored by the raiser, but substantial variation has taken place in physicochemical properties such as milk electrical conductivity, somatic cell counting, pH value, must just can detect with special physico-chemical method, and is just diagnosable as passing through pathogen or somatic cell check.Stealthy mastitis sickness rate height is 15～40 times of clinic mastitis, can develop into clinic mastitis when serious, and milk yield reduces more than 10%, can influence milk matter when more serious.
In the U.S., have at least a breast district infected in 30%～70% the cow.The incidence rate of China's mammitis of cow is higher.The survey showed that, clinic mastitis sickness rate average out to 33.41%, and stealthy mastitis average positive rate is 73.91%,, newborn chamber prevalence is 44.74%.Stealthy mastitis be in the mastitis incidence rate at most, cause the most serious a kind of disease of economic loss, seriously restricting the development of dairy.In every year, because of causing medical expense, mastitis increases, milk yield descends, cow is eliminated too early etc., makes dairy farmer and milk cattle cultivating enterprise suffer tremendous loss.It is reported that every cow of U.S. every year, the cost because of mastitis was 182 dollars, the economic loss that every cow of Denmark causes because of mastitis is 225 dollars, and wherein about 60% loss is descended by milk yield and causes.People list antibiotic in treat mammitis of cow choice drug for a long time, adopt Western medicine to treat mastitis, have obtained certain effect.A kind of dry milk cow breast filling agent that is used to prevent and treat mammitis of cow is disclosed as Chinese patent CN200410014281, contain ampicillin or amoxicillin 100-150mg, acetic acid hydrogenation ripple Buddhist nun pine 4-7mg, sodium chloride 400-550mg, vegetable oil 6-6.5ml, polysorbate 0.75-0.82ml in its every 10ml recipe quantity, surplus is a sterile purified water.Chinese patent CN200510011522 discloses a kind of breast perfusate for the treatment of mammitis of cow, and it is the solution of 2-3 with the distilled water secure ph, adds nisin then, it is tired be the 100000-500000 units per ml, and sterilization promptly.But prolonged and repeated use antibiotics western medicine makes pathogen that medicine has been produced Drug resistance easily, thereby has reduced curative effect, and has increased residues of antibiotics in the milk.
Summary of the invention
The object of the invention is to provide a kind of infusion liquid for the treatment of the stealthy mastitis of milch cow, and it is used for the treatment of, and the stealthy mastitis of milch cow is little to the milch cow zest, low-residual and good effect.
The present invention also provides the preparation method of this infusion liquid.
For achieving the above object, the present invention adopts following technical scheme:
A kind of infusion liquid for the treatment of the stealthy mastitis of milch cow, this infusion liquid is mainly made by following proportion raw material: the Radix Astragali 4000～5000g, Herba Epimedii 4000～5000g, levamisole hydrochloride 300～500g, antibacterial peptide 300～400g, glycerol 2000～3000ml, gelatin 400～500g, sodium benzoate 50～100g, this infusion liquid pH 5.5～7.5.
The preparation method of above-mentioned infusion liquid, it may further comprise the steps: 1. in the Radix Astragali and Herba Epimedii, adds the water of 8～10 times of both weight sums, decocts 2 times, 2h, 1h for the second time for the first time, collecting decoction, filtration, it is 1.20～1.25g/cm that filtrate is concentrated into density
3(can measure the density of filtrate with gravimeter) adds dehydrated alcohol to concentration of alcohol then and accounts for 70% of liquor capacity total amount, leaves standstill filtration, gets solution a behind the ethanol in the recovery filtrate; 2. add levamisole hydrochloride, antibacterial peptide in 2000～3000ml water for injection, stirring and dissolving gets solution b; 3. add glycerol in 2000～3000ml water for injection, add gelatin when being heated to 60～80 ℃, stirring and dissolving gets solution c; Again solution a, b are mixed the back and add among the solution c and stirring, add sodium benzoate, add the injection water and be settled to 10000ml, then with sodium citrate regulate pH value be 5.5～7.5(herein the consumption of sodium citrate be generally 200～300g), sterile filling is promptly.
Compare with prior art, beneficial effect of the present invention: the extractive technique of utilization modern Chinese medicine and advanced pharmaceutical technology, with the Chinese medicine and western medicine perfect adaptation, and incorporate current scientific and technological new results antibacterial peptide make can perfusion, the high-tech product of nonreactive, noresidue, it is used for the treatment of the stealthy mastitis determined curative effect of milch cow, local application's drug level height, absorption rate height, with strong points, medication is convenient, safety, thereby reach the purpose of the recessive mammitis of cow of quick treatment, be a kind ofly collect antibiotic, antiinflammatory, human body immunity improving power is the compound filling preparation of one.
The specific embodiment
The present invention is described further by the following examples, but protection scope of the present invention is not limited thereto.
Embodiment 1-5: a kind of infusion liquid for the treatment of the stealthy mastitis of milch cow, the proportioning raw materials that this infusion liquid mainly contains sees table 1 for details.
The preparation method of described infusion liquid is described in detail in detail with embodiment 1:
1. in the 4000g Radix Astragali and 5000g Herba Epimedii, add the water of 9 times of both weight sums, decocts 2 times, 2h, 1h for the second time for the first time, collecting decoction, filtration, it is 1.25g/cm that filtrate is concentrated into density
3, add dehydrated alcohol to concentration of alcohol then and account for 70% of liquor capacity total amount, leave standstill filtration, get solution a behind the ethanol in the recovery filtrate;
2. add 300g levamisole hydrochloride, 375g antibacterial peptide in 2500ml water for injection, stirring and dissolving gets solution b;
3. add 2500ml glycerol in 2500ml water for injection, add the 400g gelatin when being heated to 70 ℃, stirring and dissolving gets solution c; Stirring among the adding solution c after solution a, b being mixed again, adding the 100g sodium benzoate, adding the injection water and be settled to 10000ml, adding sodium citrate adjusting pH is 6.5, and sterile filling promptly.
The test of pesticide effectiveness:
(1) experiment material
Choose 48 of the milch cows (about 500kg) that body condition is close, make a definite diagnosis the stealthy mastitis of trouble lactation period after testing from certain large-scale cattle farm, be divided into two groups at random, be test group (product that perfusion embodiment 1 makes) and antibiotic matched group (perfusion penicillin sodium, specification: 200,000 units/prop up) 24 every group.
(2) test method and dosage: every group of twice of breast perfusion every day (every newborn chamber 20ml), 7 days is a course of treatment, continuous use 7 days, result of the test sees Table 2.
(3) detection method and criterion:
Use subclinical mastitis quick diagnosis agent (BMT) method to carry out: BMT stock solution (milch cow institute Beijing, Beijing milch cow New Tech S. R. L. provides, latent mammitis diagnosis dish and the BMT medicament is supporting buys) is diluted (now with the current) with distilled water by the volume ratio of 1:4; The milk of getting left front, left back, right front, right back 4 the breast districts of milch cow respectively detects.Earlier clean the breast district with warm water before milking, dry with soft clean wet paper towel then, squeeze earlier and removes first three milk, emulsion is extruded in the diagnosis dish, 45 ℃ of dish inclinations discard unnecessary milk, make that each newborn ware stays about 2ml milk in the diagnosis dish.Each milk sample adds the BMT stock solution 2ml after the dilution, and its horizontal sample concentric circular is shaken, on have a down dip shake 50 seconds after, judge according to somatic cell number in the milk, the results are shown in Table 2.
Criterion of cure: milk is negative, and the somatic cell number is less than 200,000 ml in the milk
-1
The improvement standard: somatic number falls now in the milk, transfers the weak positive to by strong positive, the positive;
Invalid standard: the somatic cell number does not descend in the milk.
Effective percentage is cure rate and improvement rate sum.
This test data adopts SPSS13.0 software to analyze, and analyzes with the remarkable method of minimum difference (LSD) between per two groups.As can be seen from Table 3, the matched group cure rate is 50%, and improvement rate is 62.5%; But after being to use infusion liquid of the present invention that milch cow is carried out breast perfusion, its cure rate reaches 70.8%, and improvement rate is 87.5%; And do not find untoward reaction.This shows that the therapeutic effect of test group is significantly higher than matched group (P＜0.05), infusion liquid of the present invention can be used for the treatment of the stealthy mastitis of milch cow.
Claims (2)
1. infusion liquid for the treatment of the stealthy mastitis of milch cow, it is characterized in that, this infusion liquid is mainly made by following proportion raw material: the Radix Astragali 4000～5000g, Herba Epimedii 4000～5000g, levamisole hydrochloride 300～500g, antibacterial peptide 300～400g, glycerol 2000～3000ml, gelatin 400～500g, sodium benzoate 50～100g, this infusion liquid pH 5.5～7.5.
2. the preparation method of the described infusion liquid of claim 1 is characterized in that, may further comprise the steps:
1. in the Radix Astragali and Herba Epimedii, add the water of 8～10 times of both weight sums, decocts 2 times, 2h, 1h for the second time for the first time, collecting decoction, filtration, it is 1.20～1.25g/cm that filtrate is concentrated into density
3, add dehydrated alcohol to concentration of alcohol then and account for 70% of liquor capacity total amount, leave standstill filtration, get solution a behind the ethanol in the recovery filtrate;
2. add levamisole hydrochloride, antibacterial peptide in 2000～3000ml water for injection, stirring and dissolving gets solution b;
3. add glycerol in 2000～3000ml water for injection, add gelatin when being heated to 60～80 ℃, stirring and dissolving gets solution c; Solution a, b are mixed among the back adding solution c stirring again, add sodium benzoate, add the injection water and be settled to 10000ml, regulating pH value with sodium citrate then is 5.5～7.5, and sterile filling promptly.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104938822A (en) * | 2015-06-06 | 2015-09-30 | 安徽极速时代动物营养有限公司 | Feed additive for reducing cow body cells and treating recessive mastitis and preparation method of feed additive |
| CN105597082A (en) * | 2016-01-29 | 2016-05-25 | 河南金大众生物工程有限公司 | Dairy cow breast injectant containing antimicrobial peptide and preparing method thereof |
| CN107519156A (en) * | 2017-09-19 | 2017-12-29 | 成都医学院 | The new application of citral and its derivative |
| CN108096284A (en) * | 2017-11-20 | 2018-06-01 | 山东德正乳业股份有限公司 | A kind of Biological compound preparation for preventing mastitis for milk cows and preparation method thereof |
-
2011
- 2011-01-21 CN CN 201110022938 patent/CN102068684A/en active Pending
Non-Patent Citations (1)
| Title |
|---|
| 《分析科学学报》 20071031 余剑等 离子对色谱法测定万乳康注射液中淫羊藿苷和盐酸左旋咪唑含量 第586－588页 1、2 第23卷, 第5期 * |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104938822A (en) * | 2015-06-06 | 2015-09-30 | 安徽极速时代动物营养有限公司 | Feed additive for reducing cow body cells and treating recessive mastitis and preparation method of feed additive |
| CN105597082A (en) * | 2016-01-29 | 2016-05-25 | 河南金大众生物工程有限公司 | Dairy cow breast injectant containing antimicrobial peptide and preparing method thereof |
| CN105597082B (en) * | 2016-01-29 | 2019-05-31 | 河南金大众生物工程有限公司 | A kind of cow breast injectant and preparation method thereof containing antibacterial peptide |
| CN107519156A (en) * | 2017-09-19 | 2017-12-29 | 成都医学院 | The new application of citral and its derivative |
| CN108096284A (en) * | 2017-11-20 | 2018-06-01 | 山东德正乳业股份有限公司 | A kind of Biological compound preparation for preventing mastitis for milk cows and preparation method thereof |
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Application publication date: 20110525 |