CN102988193A - Medical beautifying polypeptide capable of promoting cell regeneration and delaying skin aging - Google Patents
Medical beautifying polypeptide capable of promoting cell regeneration and delaying skin aging Download PDFInfo
- Publication number
- CN102988193A CN102988193A CN2011102784908A CN201110278490A CN102988193A CN 102988193 A CN102988193 A CN 102988193A CN 2011102784908 A CN2011102784908 A CN 2011102784908A CN 201110278490 A CN201110278490 A CN 201110278490A CN 102988193 A CN102988193 A CN 102988193A
- Authority
- CN
- China
- Prior art keywords
- skin
- peptide
- beauty treatment
- cys
- aging
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Images
Landscapes
- Cosmetics (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Abstract
The invention provides a medical beautifying polypeptide capable of promoting cell regeneration and delaying skin aging. The medical beautifying polypeptide can be used in skin care products and cosmetics in medical beautifying application, so that skin aging of a user can be delayed. The polypeptide has a sequence which consists of 13 to 20 amino acids and is of a novel sequence design by taking the sequence of a growth factor sequence as a core, and the designed novel polypeptide sequence can play an effect better than that of the growth factor.
Description
Technical field
The present invention relates to a kind ofly can promote the medical cosmetology of cell new life and delay skin aging to win peptide more, wherein this medical cosmetology wins peptide more and comprises victory peptide and pharmaceutically acceptable carrier, this beauty treatment sequence how the victory peptide is comprised of 13～20 aminoacid, this beauty treatment peptides that win have suitable effect for increase skin elasticity, minimizing dermal melanin, reinforcement skin from the master lock outlet capacity more.
Background technology
1. the reason of skin aging
The formation of skin aging is very complicated process, has involved countless physiological phenomenons.The aging origin cause of formation is as many as stars, general mainly can be divided into extrinsic factor (Exposure to Sunlight, pollution, free radical injury, smoking), and intrinsic factor (cell natural death, natural aging, hormone minimizing, immunity reduce), under inside and outside multiple aging action impact, skin will inevitably be dry, lax, produce wrinkle and follow the string, and loses gradually young gloss.
The reason of skin aging has a lot: the reduction of skin oxygen concentration, free radical injury, collagen protein reduce, cell turnover slows down, paracytic appearance, skin fat minimizings, intercellular substance shortage, Growth of Cells are stopped, hormone decline etc., all be the partly cause of skin aging, also have numerous other reasons to participate in the process of skin aging.
By and large, skin aging can be owing to following four factors:
1. the degeneration of collagen protein and elastic fiber (mainly being caused by aging, free radical and uv damage);
2. the minimizing of skin epidermis and the fat deposit impact of gravity (aging and);
3. the countenance (causing the wrinkles such as the stricture of vagina of frowning, crows-feet and decree stricture of vagina) that repeats;
4. laxity of ligament and cause the lax phenomenon of the sagging and skin of muscle.
Collagen protein and elastic fiber are the skeletons of skin texture, after long-term ultraviolet injury, and their gradually disintegrate minimizings, becoming does not have elasticity.Young skin has more moisture content and fat than aging skin, and therefore aging skin seems drier transparent, and fat people seems that wrinkle is less.In addition, skin area can continue to enlarge along with the age, and the fatty tissue of bottom reduces gradually, skin area increases, but do not have enough bone (bone also can along with the age degenerates) and fat to support, add gradually atrophy of facial muscle, so the sagging phenomenon of cutis laxa produces.
The composition of skin also can reduce gradually along with the age, and water conservation composition and structure composition such as the nerve amines of intercellular substance, hyaluronic acid, Polysaccharides, glycerol etc. exhaust that gradually collagen protein and the elastic fiber of skin are also decayed gradually.In addition, because the result of immunity variation, aging skin also is easier to occur anaphylaxis and skin irritation.
More deep discussion molecular biology: weaken year by year because DNA and RNA pass to the message of skin growth, Skin Cell can't recover to copy vigorous situation as at an early age, will begin to occur obviously aging.The ability variation of skin moisturizing is so that the sense of touch of skin has also changed.DNA and RNA message weaken may a lot of reasons, comprises inflammatory response that long-term ultraviolet injury, free radical cause etc.
When skin surface exsiccation or irriate, depression can appear in the surface, and this is so-called little microgroove.They can allow skin seem wrinkle, but little microgroove is not wrinkle, as long as use wetting agent just can improve.If extremely dry skin does not use wetting agent, even dry rubescent phenomenon also can appear, that be because intercellular substance impaired due to.Intercellular substance is comprised of glycerol, cholesterol, nature moisturizing factor, mucopolysaccharide and other water-retaining agent.Water can not recover the health of intercellular substance, and lubricant, oils and fats and nature moisturizing factor then have this function.Yet if let go unchecked or the skin cell serious aging, these little microgrooves will become wrinkle, just can not improve with wetting agent afterwards.
Avoid the over-drying microgroove that causes, should appropriateness and regular exfoliation removes the aging cell of skin surface, allow the cell of plentiful health manifest, the skin behind the exfoliation has the sensation of taking on an entirely new look.Protect skin at clean healthy skin with sun-proof, antioxidation, irritation product, keep the water holding capacity of skin with skin care products again, microgroove can't form naturally.
2. slow down the method-somatomedin of skin aging
Somatomedin is a kind of indispensable functional protein in the body, Main Function is after the accepter of cell surface is combined, irritation cell produces a series of cell proliferation and differentiation, normal cell wants hypertrophy that the stimulation of somatomedin is arranged, and different Skin Cells also needs the stimulation of specific somatomedin to accelerate to upgrade and regeneration.The skin growth factor can provide the skin nutrient; delay skin aging, promotion skin repair; it is the optimum activity composition of skin nursing; can control or the regulation of skin ager process; the protection damaged skin; delay skin aging, significant to the 26S Proteasome Structure and Function, the normal physiological activity of keeping skin and the metabolism that keep normal skin.If skin does not have the stimulation of somatomedin, can't be newborn.
Somatomedin is few at people's in-vivo content, but biological activity is high, has multiple important biological effect and physiological function.Multiple somatomedin is relevant with growth, division, differentiation, propagation and the migration of Skin Cell, and they can provide the skin nutrient, delay skin aging or promotion skin repair.Skin aging is the outward manifestation of human senility, is because endogenous and the coefficient result of composite factor such as exogenous.With advancing age, skin aging more and more obvious, main manifestations be xerosis cutis, coarse, wrinkle, tarnish, elasticity reduce, pale, lax, even atrophoderma, crowfoot cracks and senile plaque etc. appear.Solar radiation or other environmental factors also can cause skin aging and impaired, reduce skin activity.Aging appearance and the image that has not only affected human body of skin, but also the physiological function that has seriously hindered human body skin, even also can cause the serious pathologic of skin to change.Somatomedin is the optimum activity composition of skin nursing, can control or the regulation of skin ager process, and the protection damaged skin, delay skin aging, significant to the 26S Proteasome Structure and Function, the normal physiological activity of keeping body and the metabolism that keep normal skin.
With advancing age, somatomedin secretory volume in vivo also reduces gradually, skin aging more and more obvious, and that skin becomes is dry, coarse, wrinkle, tarnish, elasticity minimizing, pale, lax, even atrophoderma, crowfoot cracks and senile plaque etc. occur.In addition, with advancing age, the blood flow of skin also reduces gradually, and nutrient and oxygen are not easy to deliver to epiderm skin and corium, also can accelerate the aging of skin.
From 2000, the somatomedin that people begin some are had specific function added in the cosmetics skin care products, as: EGF, bFGF, aFGF, TGF-β.Wherein EGF (epidermal growth factor) and FGF use two kinds more widely, wish to reach the anti-ageing effect that smoothes away wrinkles, increases elasticity, delaying aging by the high activity of somatomedin.
So the continuous input research of past scientist wishes to see through special processing or processing procedure, expectation is found out and is more suitable for the somatomedin kenel that skin absorbs.Except the cutaneous absorption of molecular size range meeting of somatomedin, the source of somatomedin and concentration also are the important evidence that depends on its quality good and the bad.Somatomedin must see through after the gene recombinaton with antibacterial or yeast manufacturing, the source of strain and the concentration of synthesizing and purifying, the safety in the activity that all can affect somatomedin and the use.
Utilizing biotechnology to extract effective skin care products composition from natural product is the leading role of skin care products in recent years, especially the skin care products that comprises somatomedin, as with epidermal growth factor (Epidermal Growth Factor, EGF) with the skin care products such as fiber mother cell growth factor (Fibroblast Growth Factors, FGF).
Yet somatomedin mainly comes from fermentation, extraction in the materials such as cattle and sheep Placenta Hominis, serum extraction, baby's foreskin or escherichia coli, and purity is generally less than 70%, the more easy allergy of impurity, and the source is not clear, easily has disease to infect and the residual risk of endotoxin.Even so, but units up to ten thousand easily of one bottle of the somatomedin skin care products of on the market import.
In addition, the protein that somatomedin is comprised of aminoacid, general molecular weight is all greater than 6.2kD, but the receptible molecular weight of human body pore is below the 3kD, present external existing experiment confirm just can enter skin less than 3kD and play a role.Therefore, the somatomedin that comprises the major part such as fiber mother cell growth factor all can't enter skin corium because molecular weight is too large and play a role.
3. slow down the method for skin aging-weak point and win peptide
The source of obtaining because of somatomedin is animality, use somatomedin may have the doubts such as skin allergy, inflammation, red and swollen suppuration, so the short peptide that wins in the streets, for example: Wushengtai (500Dalton), Argireline (600Dalton), just can be popular; Although it is shorter to win peptide, and its biological activity can not show a candle to somatomedin, and is shorter because winning peptide, is absorbed by the skin easily, therefore still helpful to skin elasticity.
In the market more well known two kinds win peptides be Wushengtai (Palmitoyl Pentapeptide 3, MatrixylTM) with Argireline (Acetyl Hexapeptide 3,
Wushengtai is the raw material that French SEDERMA company produces, and mainly is to form a fat-soluble victory peptide by five amino acid and Palmic acid, and experiment in vitro shows the function of strengthening the 4th type (Type IV) collagen protein synthesis.Argireline then is the raw material that Spain LIPOTEC company produces, and is called again the Argireline element, its effect of therefore named think of justice be similar to injection Botox (Botulinum Toxin,
The mechanism of utilizing its block nerves Transmitter acetylcholine (acetylcholine) to discharge suppresses the contraction of muscle, reaches the effect of eliminating dynamic lines.
No matter be Wushengtai or Argireline, it not existing aminoacid sequence structure in the human body, but by the molecule of the synthetic gained of science and technology, Wushengtai whether can be single-minded to skin corium fibroblast (fibroblast) and effectively produce excitation and Argireline whether can the block nerves conduction, still lack complete its effect of Experiment and clinic presentations test proof at present.According to above-mentioned, the skin care products that has desalination microgroove and increase skin elasticity concurrently is the problem that beauty treatment industry wish solves always.
For instance, on the market for prevention or improve cutis laxa and wrinkle two large classes victory peptides are arranged, a kind of is to reach by physical property to allow immediately consolidation of skin, but this mode can't really solve the lax problem of skin aging, and the time that therefore can keep skin-tightening is quite short.Another kind is the Main Ingredients and Appearance collagen protein that replenishes skin corium, it is to utilize to win peptide compounds to promote the collagen protein hypertrophy, winning peptide compounds is the above aminoacid that form of two aminoacid, is commonly referred to as and wins peptide or polypeptide, and its effect is different from putting in order according to the aminoacid classification that forms.Generally speaking, the aminoacid quantity that wins peptide is few, and molecule is little and enter easily skin, and easily synthetic, cost is also lower, but when the aminoacid quantity of victory peptide very little the time, can't form stereochemical structure and not have protein active, such as palmitoyl tripeptide 3, Wushengtai or Argireline etc. all be; Otherwise when the aminoacid quantity that wins peptide was too many, molecule can't enter skin too greatly again, can't reach beauty functions.
So far at present have following disappearance about the known techniques of slowing down skin aging: 1, the growth factor molecule amount is too large, can't be via smearing the skin effect that enters; 2, somatomedin mainly comes from fermentation, extraction in the materials such as cattle and sheep Placenta Hominis, serum extraction, baby's foreskin or escherichia coli, and purity is generally less than 70%, the more easy allergy of impurity, and the source is not clear, easily has disease to infect and the residual risk of endotoxin; 3, the somatomedin price is very expensive; 4, somatomedin has the possible doubt of carcinogenic and other side effect; 5, the activity of somatomedin is difficult for preserving; 6, win peptide ammino acid quantity very little the time, can't form stereochemical structure and do not have protein active; 7, winning peptide is not existing aminoacid sequence structure in the human body, but by synthetic point of science and technology, whether Wushengtai can block nerves conduct effect and the Argireline of skin corium fibroblast, still lacks complete its effect of Experiment and clinic presentations test proof at present.
Summary of the invention
Because the somatomedin of present known beauty treatment usefulness is the animality source, therefore be easy to cause allergic symptom, can have a negative impact for health, if with palmitoyl tripeptide 3, Wushengtai or Argireline replace, then its beauty treatment effect is not good again, therefore the present invention develops and synthetic a kind of can promote the medical cosmetology of cell new life and delaying human body skin aging to win peptide more, synthesized by 13～20 specific amino acids, its molecular weight is about 2.4KD, be of moderate size, can be via smearing the skin effect that enters, and should beauty treatment win that peptides are active to be preserved easily more, add in the product at normal temperatures through still having activity more than 24 months, can reach the effect of beauty treatment.
Slow down skin aging and mainly contain following mechanism:
1, naturality is aging follows the aging of whole body organ to carry out synchronously, keeps the life of rule, and joyful mood, balanced diet, sufficient sleep, suitable motion are the only effective means of anti-natural cause aging.
2, moisturizing: skin moisturizing ability and day, decrescence the skin hydropenia caused ductility to reduce, and cutin is piled up, microgroove occurs, and smearing moisturizer is the task of top priority.Commercially available moisturizer is various, and effect quality gap is quite large, and on the whole effect can be divided into four: 1) direct airtight epidermis, do not allow water evaporates; 2) have and absorb in the atmosphere moisture content in the ability of epidermis; 3) replenish the nature moisturizing factor that disappears on the skin; 4) provide the composition that prevents that skin moisture scatters and disappears.
3, the skin care products of activation aging skin regeneration: by regeneration skin corium structure, can have concurrently improve that cutis laxa is aging, microgroove, the dark Shen of the colour of skin.
4, check melanin: ultraviolet not only can generate by stimulation melanin, more may cause human body skin to become pitch-dark, thick and do, wears out, and impels easily skin aging ahead of time, the last even skin carcinoma of deriving.Therefore check melanin is the injury of prevention photoaging.
The present invention develops synthetic beauty treatment polypeptide can be increased simultaneously skin elasticity, suppresses dark red pigment and increase skin from the master lock outlet capacity.
The aminoacid sequence title defining contrast table that the present invention quotes:
| English | Chinese | Abbreviation |
| Glycine | Glycine | Gly(G) |
| Alanine | Alanine | Ala(A) |
| Valine | Valine | Val(V) |
| Leucine | Leucine | Leu(L) |
| Isoleucine | Isoleucine | Ile(I) |
| Serine | Serine | Ser(S) |
| Threonine | Threonine | Thr(T) |
| Cystine | Cysteine | Cys(C) |
| Methionine | Methionine | Met(M) |
| Proline | Proline | Pro(P) |
| Lysine | Lysine | Lys(K) |
| Arginine | Arginine | Arg(R) |
| Phenylalanine | Phenylalanine | Phe(F) |
| Tyrosine | Tyrosine | Tyr(Y) |
| Tryptophan | Tryptophan | Trp(W) |
| Histidine | Histidine | His(H) |
| Aspartate | Aspartic acid | Asp(D) |
| Glutamate | Glutamic acid | Glu(E) |
| Asparagine | N | Asn(N) |
| Glutamine | Glutamine | Gln(Q) |
The amino acid whose classification of content indication of the present invention:
1, fatty amino acids (Aliphatic) comprising: alanine (Alanine, A), isoleucine (Isoleucine, I), leucine (Leucine, L), valine (Valine, V), proline (Proline, P).
2, aromatic amino acid class (Aromatic) comprising: phenylalanine (Phenylalanine, F), tryptophan (Tryptophan, W),, tyrosine (Tyrosine, Y).
3, acidic amino acid class (Acidic) comprising: aspartic acid (Aspartic acid, D), glutamic acid (Glutamic acid, E).
4, basic amino acid class (Basic) comprising: arginine (Arginine, R), histidine (Histidine, H), lysine (Lysine, K).
5, hydroxyl amino acids (Hydroxylic) comprising: serine (Serine, S),, threonine (Threonine, T).
6, sulfur-containing amino acid class (Sulfur containing) comprising: cysteine (Cysteine, C), methionine (Methionine, M).
7, amidoamino acid class (Amidic) comprising: N (Asparagine, N), glutamine (Glutamine, Q).
One of the object of the invention provides a kind of medical cosmetology polypeptide that can promote cell new life and delaying human body skin aging, this sequence is that acquisition is from the aminoacid sequence of human EGF somatomedin, this human growth factor's aminoacid sequence is: SEQ ID No.1 is specially: Asn-Ser-Asp-Ser-Glu-Cys-Pro-Leu-Ser-His-Asp-Gly-Tyr-Cys-Leu-His-Asp-Gly-Val-Cys-Met-Tyr-Ile-Glu-Ala-Leu-Asp-Lys-Tyr-Ala-Cys-Asn-Cys-Val-Val-Gly-Tyr-Ile-Gly-Glu-Arg-Cys-Gln-Tyr-Arg-Asp-Leu-Lys-Trp-Trp-Glu-Leu-Arg.
The aminoacid sequence of the polypeptide that the present invention synthesizes is take SEQ ID No.2 as core sequence, and the basic acid sequence of SEQ IDNo.2 is specially: Ile-Glu-Ala-Leu-Asp-Lys-Tyr-Ala.
Another object of the present invention provides a kind of beauty treatment polypeptide, this peptide sequence is central take SEQ ID No.2 (Ile-Glu-Ala-Leu-Asp-Lys-Tyr-Ala) as core sequence, and at the N of this core sequence end and the additional aminoacid sequence through design of C end: Cys-Pro-R1n1-Cys-R2n2-R3n3-Ile-Glu-Ala-Leu-Asp-Lys-Tyr-A la-Cys-R4n4-Cys-R5n5-R6n6-R7n7, this wins the R1 of peptide sequence, R2, R3, R4, R5, R6 and R7 are substituent group, this substituent group is selected from glycine, fatty aminoacid, aromatic amino acid, acidic amino acid, basic amino acid, hydroxy-amino-acid, in sulfur-containing amino acid and the amidoamino acid one or more, and n1 wherein, n2, n3, n4, n5, n6 and n7 are substituent number, and this number distinctly independently equals 0 or 1.
Proline (Proline) is the most outstanding aminoacid of structure in the middle of all aminoacid, and its side group contains three carbon, after being set out by a carbon, goes back to engage with the nitrogen-atoms on next door, forms five annulus; Rely on such structure, the long-chain that peptide is won is transferred into fixed angle; Therefore should win the central Cys of peptide sequence and Pro on the configuration that beauty treatment polypeptide of the present invention wins, very important power of influence was arranged.Therefore, can allow 13～20 amino acid whose victory peptides form special stereochemical structure, can assist this to win peptide and be combined with growth factor acceptor, the function that performance is better with somatomedin.
Another object of the present invention provides a kind of beauty treatment peptides that win more, it wins peptide sequence and is preferably SEQ ID No.3, and aminoacid sequence is specially: Cys-Pro-Leu-Cys-Met-Tyr-Ile-Glu-Ala-Leu-Asp-Lys-Tyr-Ala-Cys-Asn-Cys-Glu-Lys-Arg.
The invention relates to a kind of medical cosmetology polypeptide that can promote cell new life and delaying human body skin aging, this polypeptide is one section aminoacid sequence similar to the human body skin somatomedin, obtain via synthetic volume production, energy chafe corium fabric blast cell is newborn, adjust the newborn cycle of skin, compose the skin of living, this wins simultaneously, also easier preservation more stable than general somatomedin of peptide, more be difficult for losing activity, also do not have doubt and the risk of life-time service.
The victory peptide that the present invention synthesized is comprised of 13～20 specific amino acids, obtain via the synthetic volume production of aminoacid, have the somatomedin structure similar to the Cell binding part, add that molecule is little, equal the somatomedin of scaled down version, can enter skin via smearing through skin penetrance experiment confirm, enter skin corium and fibroblast combination, Promote cell's growth also produces a large amount of collagen protein, adjusts the newborn cycle of skin, composes the skin of living.Microgroove and wrinkle be can desalinate fast, skin elasticity and water conservation degree increased.
Description of drawings
Fig. 1 be the test group beauty treatment peptide-FITC (Fluorescein isothiocyanate) of victory and BSA (bovine serum albumin)-FITC more be applied in nude mice behind after 10 minutes in the state diagram of visible light and fluorescence; Wherein,
Figure A is that matched group BSA-FITC smears rear 10 minutes visible light state diagram;
Figure B is that matched group BSA-FITC smears rear 10 minutes in the fluorescence state diagram;
Figure C is that the test group beauty treatment peptide-FITC of victory smear rear 10 minutes visible light state diagram more;
Figure D is that the test group beauty treatment peptide-FITC of victory smear rear 10 minutes fluorescence state diagram more.
Fig. 2 is that the experimenter uses the beauty treatment skin elasticity variation diagrams that win the blank cream of peptide more.
Fig. 3 is that the experimenter uses the beauty treatment melanin that win the blank cream of peptide to reduce ratio chart more.
Fig. 4 is that the experimenter uses the beauty treatment skin percutaneous moisture loss rate figure that win the blank cream of peptide more.
The specific embodiment
The following example is non-limiting and only as the typical example of the present invention in each side.
Embodiment 1: the skin penetration fluorescent test
Present embodiment with sequence SEQ ID No.3 (Cys-Pro-Leu-Cys-Met-Tyr-Ile-Glu-Ala-Leu-Asp-Lys-Tyr-Ala-Cys-Asn-Cys-Glu-Lys-Arg) as experiment material, take nude mice as model animal, be divided into two groups of test group, respectively beauty treatment of the present invention won peptide-FITC and BSA-FITC (matched group) is applied in each pilot region of nude mice skin of back more; According to every group of each nude mice of EXPERIMENTAL DESIGN, observed 10 minutes to 250 minutes, each substances enters the situation of skin, two nude mices smear number of times by 1 time to maximum 9 times, every minor tick 30 minutes, each is organized in smearing for the last time substances after 10 minutes, sacrifices animal with excess carbon dioxide, the dermatological specimens of each trial zone to be vertically carrying out frozen section, and with the fluorescence distribution of fluorescence microscope FITC (Fluorescein isothiocyanate).
Fluorescence distribution figure as shown in Figure 1, result of the test shows, the test group beauty treatment peptide-FITC pilot regions that win more, can observe green fluorescence at epidermis and skin corium, and therefore the signal of fluorescence can learn that mainly from the skin corium of hair follicle and its below the beauty treatment peptides that win can enter skin corium via hair follicle more; Matched group BSA-FITC pilot region then only fluorescence occurs in cuticle region, and visible matched group BSA-FITC can't penetrate epidermal area.Therefore judge that the test group beauty treatment peptide-FITC of victory can slowly enter skin corium via hair follicle more.
Embodiment 2: skin elasticity is measured
Present embodiment with sequence SEQ ID No.3 (Cys-Pro-Leu-Cys-Met-Tyr-Ile-Glu-Ala-Leu-Asp-Lys-Tyr-Ala-Cys-Asn-Cys-Glu-Lys-Arg) as experiment material, assistant is with suitable prescription adjuvant, prescription is spread upon volunteer's skin, the volunteer selects 30～60 years old left and right sides women and male totally 20 experimenters, naked skin test (measuring again after 20 minutes behind the washed) is all adopted in all tests, the beauty treatment of use interpolation 0.01% wins the blank cream of peptide more, and the testing time was 9 weeks.
Utilize C+K
MPA 580 analyzers detect.Measuring condition is fixed on relative humidity 40～60% and room temperature is to carry out under 21 ℃ ± 1 ℃, measure the mensuration that probe carries out skin elasticity with MPA 580 skin elasticitys, its result analyzes with the R parameter, and with 0～100% unit representation, each position is measured 3 times, averaged.Survey once every 1 week, totally 3 weeks.Therefrom selected 6 experimenters continue to follow the trail of to 9 weeks (surveying once every 3 weeks) again.
The skin elasticity test result as shown in Figure 2, result of the test shows that the experimenter uses the beauty treatment skin elasticitys that win the blank cream of peptide to change more, the experimenter uses the beauty treatment rear skin elasticitys of three weeks of blank cream that win peptide namely to be significantly improved more, as shown in table 1, continue to use to nine weeks, the skin elasticity of eye tail place and forehead has increased by 49% and 36% before using.
Table 1, experimenter use the beauty treatment skin elasticitys that win the blank cream of peptide to change more
Embodiment 3: reduce melanin and measure
Present embodiment with sequence SEQ ID No.3 (Cys-Pro-Leu-Cys-Met-Tyr-Ile-Glu-Ala-Leu-Asp-Lys-Tyr-Ala-Cys-Asn-Cys-Glu-Lys-Arg) as experiment material, assistant is with suitable prescription adjuvant, prescription is spread upon volunteer's skin, the volunteer selects 30～60 years old left and right sides women and male totally 20 experimenters, naked skin test (measuring again after 20 minutes behind the washed) is all adopted in all tests, the beauty treatment of use interpolation 0.01% wins the blank cream of peptide more, and the testing time was 9 weeks.
Utilize C+K
MPA 580 analyzers detect.Measuring condition is fixed on relative humidity 40～60% and room temperature is to carry out under 21 ℃ ± 1 ℃, with
The dark red pigment probes of MX 18 skins (probe) are tested, and its result is with 0～999 unit representation, and each position is measured 3 times, averaged.Survey once every 1 week, totally 3 weeks.Therefrom selected 6 experimenters continue to follow the trail of to 9 weeks (surveying once every 3 weeks) again.
The melanin measurement result as shown in Figure 3, test result shows that the experimenter uses the beauty treatment rear melanin of three weeks of blank cream that win peptide that significant inhibition is namely arranged more, as shown in table 2, continue to use to nine weeks, the melanin ratio had reduced respectively-15% ,-48% ,-25% and-26% before the melanin at left eye bag, forehead, right cheekbone and left cheekbone place used the blank cream that wins peptides of improving looks more.
Table 2 experimenter uses the beauty treatment melanin that win the blank cream of peptide to reduce ratio more
| Before the use | After three weeks | After six weeks | After nine weeks | |
| The |
0％ | -4％ | -10％ | -15 |
| Forehead | ||||
| 0％ | -12％ | -30％ | -48％ | |
| |
0％ | -10％ | -17％ | -25％ |
| |
0％ | -9％ | -19％ | -26％ |
Embodiment 4: the test of skin percutaneous moisture loss
Present embodiment with sequence SEQ ID No.3 (Cys-Pro-Leu-Cys-Met-Tyr-Ile-Glu-Ala-Leu-Asp-Lys-Tyr-Ala-Cys-Asn-Cys-Glu-Lys-Arg) as experiment material, assistant is with suitable prescription adjuvant, prescription is spread upon volunteer's skin, the volunteer selects 30～60 years old left and right sides women and male totally 20 experimenters, naked skin test (measuring again after 20 minutes behind the washed) is all adopted in all tests, the beauty treatment of use interpolation 0.01% wins the blank cream of peptide more, and the testing time was 9 weeks.
Utilize C+K
MPA 580 analyzer detection of skin percutaneous moisture losses.Measuring condition is fixed on relative humidity 40～60% and room temperature is to carry out under 21 ℃ ± 1 ℃, with
TWEL TM 300 skin percutaneous moisture losses probes (probe) are tested, and its result is with 0～320g/hm
2Unit representation, each position is measured 1 time.Survey once every 1 week, totally 3 weeks.Therefrom selected 6 experimenters continue to follow the trail of to 9 weeks (surveying once every 3 weeks) again.
Skin percutaneous moisture loss rate measurement result as shown in Figure 4, test result shows that the experimenter uses the beauty treatment rear skin percutaneous moisture loss rates of three weeks of blank cream that win peptide that obvious decline is arranged more, representing beauty treatment of the present invention wins peptide more and has moistening effect, as shown in table 3, continue to use to nine weeks, skin percutaneous moisture loss rate had reduced respectively-29% ,-55% ,-45% and-45% before forehead, left eye tail, left cheekbone and left decree stricture of vagina used the blank cream that wins peptides of improving looks more.
Table 3 skin percutaneous moisture loss rate
| Before the use | After three weeks | After six weeks | After nine | |
| Forehead | ||||
| 0％ | -25％ | -31％ | -29％ | |
| The |
0％ | -31％ | -50％ | -55％ |
| |
0％ | -32％ | -38％ | -45％ |
| Left decree stricture of |
0％ | -26％ | -38％ | -45％ |
Claims (8)
1. one kind can promote the medical cosmetology of cell new life and delaying human body skin aging to win peptide more, it is characterized in that comprising victory peptide and the pharmaceutically acceptable carrier with the aminoacid sequence shown in the SEQ ID NO.2.
2. one kind can promote the medical cosmetology of cell new life and delaying human body skin aging to win peptide more, it is characterized in that comprising to win peptide sequence SEQ ID NO.2 one of is derived by core and is won peptide sequence and pharmaceutically acceptable carrier, wherein, described one of derive wins peptide sequence and comprises:
Cys-Pro-R1n1-Cys-R2n2-R3n3-Ile-Glu-Ala-Leu-Asp-Lys-Tyr-Ala-Cys-R4n4-Cys-R5n5-R6n6-R7n7
Wherein R1, R2, R3, R4, R5, R6 and R7 are substituent group, described substituent group is selected from one or more in glycine, fatty aminoacid, aromatic amino acid, acidic amino acid, basic amino acid, hydroxy-amino-acid, sulfur-containing amino acid and the amidoamino acid, and n1, n2, n3, n4, n5, n6 and n7 are substituent number, and this number independently equals respectively 0 or 1.
3. beauty treatment as claimed in claim 2 wins peptide more, it is characterized in that when R1 be Leu, R2 is Met, R3 is Tyr, R4 is Asn, R5 is Glu, R6 is Lys, R7 is Arg, and n1, n2, n3, n4, n5, n6 and n7 be at 1 o'clock, described victory peptide sequence is SEQ No.3.
4. beauty treatment wins peptide more as described in arbitrary such as claim 1-3, it is characterized in that described reducing human skin aging refers to slow down moisture loss, reduces melanin or promotes skin elasticity.
5. beauty treatment wins peptide more as described in arbitrary such as claim 1-3, it is characterized in that described carrier is one or more in the base, interfacial agent, suspending agent, gellant, adjuvant, antiseptic, antioxidant, stabilizing agent, coloring agent, spice of excipient, diluent, thickening agent, filler, bonding agent, disintegrating agent, lubricant, oils and fats or non-oils and fats.
6. beauty treatment wins peptide more as described in arbitrary such as claim 1-3, it is characterized in that it is to exist with one or more forms in emulsion, cream, quintessence oil, skin care products, cosmetics, pharmaceuticals, pharmaceutical medicine, diet product, special used food, oral powder, oral solutions and the oral lozenge that described beauty treatment wins peptide more.
7. one kind can promote that cell is newborn and delay the aging method of human skin, it is characterized in that the arbitrary described beauty treatment of the claim 1-3 of effective dose is won peptide more is transmitted in human body.
8. method as claimed in claim 7 is characterized in that described transmission method is to transmit through the mode of oral, percutaneous absorption, injection or suction.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201110278490.8A CN102988193B (en) | 2011-09-19 | 2011-09-19 | Medical beautifying polypeptide capable of promoting cell regeneration and delaying skin aging |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201110278490.8A CN102988193B (en) | 2011-09-19 | 2011-09-19 | Medical beautifying polypeptide capable of promoting cell regeneration and delaying skin aging |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN102988193A true CN102988193A (en) | 2013-03-27 |
| CN102988193B CN102988193B (en) | 2014-07-30 |
Family
ID=47917628
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201110278490.8A Expired - Fee Related CN102988193B (en) | 2011-09-19 | 2011-09-19 | Medical beautifying polypeptide capable of promoting cell regeneration and delaying skin aging |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN102988193B (en) |
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105982825A (en) * | 2015-02-13 | 2016-10-05 | 普生股份有限公司 | Use of composition in production of cosmetics with moisturizing function |
| CN107098951A (en) * | 2016-02-23 | 2017-08-29 | 肌活丽学创研所股份有限公司 | Reduce victory peptide, the method and composition of melanin content |
| CN107325155A (en) * | 2017-06-29 | 2017-11-07 | 常州市千康生物科技有限公司 | A kind of anti-oxidant ultrashort peptide Mapin WH2, its preparation method and application |
| CN107365355A (en) * | 2017-08-07 | 2017-11-21 | 温州千瑞生物科技有限公司 | The peptide of anti-wrinkle and the cosmetics for including it |
| TWI627185B (en) * | 2017-06-19 | 2018-06-21 | 劉玉春 | Anti-aging peptide, its composition and method of using the same |
| CN108714111A (en) * | 2018-05-05 | 2018-10-30 | 杨永鹏 | A kind of cell-penetrating peptide-acetyl group Argireline nano-emulsion and preparation method thereof |
| CN111632127A (en) * | 2019-02-14 | 2020-09-08 | 三凡生技研发股份有限公司 | Short-chain peptide composition and application thereof in skin protection |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1210145A (en) * | 1997-08-29 | 1999-03-10 | 中国医学科学院基础医学研究所 | Engineering bacteria for epidermal growth factor and preparation of epidermal growth factor by using this bacteria |
| CN1865286A (en) * | 2005-05-17 | 2006-11-22 | 北京上地新世纪生物医药研究所有限公司 | Double function epidermal growth factor and its preparation method and uses |
| CN101077889A (en) * | 2006-05-25 | 2007-11-28 | 生立生物科技股份有限公司 | Polypeptide similar to growth factor and maintenance product |
-
2011
- 2011-09-19 CN CN201110278490.8A patent/CN102988193B/en not_active Expired - Fee Related
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1210145A (en) * | 1997-08-29 | 1999-03-10 | 中国医学科学院基础医学研究所 | Engineering bacteria for epidermal growth factor and preparation of epidermal growth factor by using this bacteria |
| CN1865286A (en) * | 2005-05-17 | 2006-11-22 | 北京上地新世纪生物医药研究所有限公司 | Double function epidermal growth factor and its preparation method and uses |
| CN101077889A (en) * | 2006-05-25 | 2007-11-28 | 生立生物科技股份有限公司 | Polypeptide similar to growth factor and maintenance product |
Cited By (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105982825A (en) * | 2015-02-13 | 2016-10-05 | 普生股份有限公司 | Use of composition in production of cosmetics with moisturizing function |
| CN105982825B (en) * | 2015-02-13 | 2018-12-14 | 沛进生命科学公司 | A kind of purposes of composition in the cosmetics that preparation has moisture-keeping functions |
| CN107098951A (en) * | 2016-02-23 | 2017-08-29 | 肌活丽学创研所股份有限公司 | Reduce victory peptide, the method and composition of melanin content |
| CN107098951B (en) * | 2016-02-23 | 2020-08-04 | 肌活丽学创研所股份有限公司 | Peptides, methods and compositions for reducing melanin content |
| TWI627185B (en) * | 2017-06-19 | 2018-06-21 | 劉玉春 | Anti-aging peptide, its composition and method of using the same |
| CN107325155A (en) * | 2017-06-29 | 2017-11-07 | 常州市千康生物科技有限公司 | A kind of anti-oxidant ultrashort peptide Mapin WH2, its preparation method and application |
| CN107365355A (en) * | 2017-08-07 | 2017-11-21 | 温州千瑞生物科技有限公司 | The peptide of anti-wrinkle and the cosmetics for including it |
| CN108714111A (en) * | 2018-05-05 | 2018-10-30 | 杨永鹏 | A kind of cell-penetrating peptide-acetyl group Argireline nano-emulsion and preparation method thereof |
| CN108714111B (en) * | 2018-05-05 | 2022-05-24 | 杨永鹏 | Cell-penetrating peptide-acetyl hexapeptide nanoemulsion and preparation method thereof |
| CN111632127A (en) * | 2019-02-14 | 2020-09-08 | 三凡生技研发股份有限公司 | Short-chain peptide composition and application thereof in skin protection |
| CN111632127B (en) * | 2019-02-14 | 2022-07-05 | 三凡生技研发股份有限公司 | Short-chain peptide composition and application thereof in skin protection |
Also Published As
| Publication number | Publication date |
|---|---|
| CN102988193B (en) | 2014-07-30 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102988193B (en) | Medical beautifying polypeptide capable of promoting cell regeneration and delaying skin aging | |
| CN104968360B (en) | For treatment and/or nursing skin, the compound and their make-up and beauty or pharmaceutical composition of hair and/or mucous membrane | |
| KR102265730B1 (en) | cosmetic composition for skin wrinkle improvement and whitening facial skin | |
| CN108495646A (en) | For treating and/or the compound of nursing skin, hair, nail and/or mucous membrane | |
| CN110997694A (en) | Compounds useful for the treatment and/or care of the skin, hair, nails and/or mucous membranes | |
| CN109350595A (en) | A kind of cosmetics and its preparation method and application containing bird's nest active extract | |
| EP3746041A1 (en) | Use of cyclic peptides in cosmetic | |
| CN110072875A (en) | For treat and/or nursing skin, hair, nail and/or mucous membrane compound | |
| JPWO2004016236A1 (en) | Cosmetics | |
| CN109152727B (en) | Peptides and sugar hydrolysates of cocoa beans, cosmetic compositions containing them and cosmetic use of the cosmetic compositions | |
| ES2769268T3 (en) | Synergistic extract of Palmaria palmata | |
| CN108379215A (en) | A kind of cosmetic composition for having effects that the freeze-drying compound powder of skin whitening and including the freeze-drying compound powder | |
| CN107496310A (en) | A kind of facial treatment essence liquid and preparation method thereof | |
| CN107365355B (en) | Anti-wrinkle peptide and cosmetic comprising same | |
| CN111888279B (en) | Method for promoting collagen production and corresponding medicine or cosmetic | |
| KR20150120426A (en) | Cosmetic composition containing a brown alga extract, a yeast extract and ascorbic acid | |
| JP2005306831A (en) | Skin care preparation for external use | |
| JP2023526854A (en) | Skin care products containing a protein matrix | |
| CN114344188A (en) | Hair loss prevention and hair growth promoting composition and preparation method thereof | |
| CN111961119B (en) | Application of polypeptide in preparation of medicine or cosmetic for promoting collagen secretion | |
| CN104323966A (en) | Golden hydrotherapy composition and using method thereof | |
| CN107496194A (en) | A kind of surfactant and preparation method thereof | |
| KR102382002B1 (en) | A multifunctional cosmetic composition for elasticity, anti-wrinkle, inhibiting tyrosinase comprising peptide complex and natural ingredients | |
| CN109730943A (en) | A kind of anti-wrinkle composition | |
| KR102281606B1 (en) | A multifunctional cosmetic composition for elasticity, anti-wrinkle, inhibiting tyrosinase comprising peptide complex |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| ASS | Succession or assignment of patent right |
Owner name: KANGLIWEI BIOMEDICAL TECHNOLOGY CO., LTD. Free format text: FORMER OWNER: DINGXIANG BIOLOGICAL PHARMACEUTICAL TECHNOLOGY CO., LTD. Effective date: 20130608 |
|
| C41 | Transfer of patent application or patent right or utility model | ||
| TA01 | Transfer of patent application right |
Effective date of registration: 20130608 Address after: Taiwan, China Applicant after: DINGXIANG SHENGYI TECHNOLOGY CO., LTD. Address before: Taiwan, China Applicant before: Dingxiang Shengyi Technology Co., Ltd. |
|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| ASS | Succession or assignment of patent right |
Owner name: DINGXIANG BIOLOGICAL AND MEDICAL TECHNOLOGY CO., L Free format text: FORMER OWNER: KANGLIWEI BIOMEDICAL TECHNOLOGY CO., LTD. Effective date: 20150428 |
|
| C41 | Transfer of patent application or patent right or utility model | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20150428 Address after: Taiwan, China Patentee after: Ding Xiang Sheng medical Polytron Technologies Inc Address before: Taiwan, China Patentee before: DINGXIANG SHENGYI TECHNOLOGY CO., LTD. |
|
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20140730 Termination date: 20150919 |
|
| EXPY | Termination of patent right or utility model |