CN107365355A - The peptide of anti-wrinkle and the cosmetics for including it - Google Patents
The peptide of anti-wrinkle and the cosmetics for including it Download PDFInfo
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- CN107365355A CN107365355A CN201710665855.XA CN201710665855A CN107365355A CN 107365355 A CN107365355 A CN 107365355A CN 201710665855 A CN201710665855 A CN 201710665855A CN 107365355 A CN107365355 A CN 107365355A
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- China
- Prior art keywords
- peptide
- seq
- skin
- wrinkle
- cosmetics
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Classifications
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K7/00—Peptides having 5 to 20 amino acids in a fully defined sequence; Derivatives thereof
- C07K7/04—Linear peptides containing only normal peptide links
- C07K7/06—Linear peptides containing only normal peptide links having 5 to 11 amino acids
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/64—Proteins; Peptides; Derivatives or degradation products thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/08—Anti-ageing preparations
Abstract
The present invention relates to the active peptide with anti-wrinkle effect and the cosmetics for including it.Specifically, the invention discloses SEQ ID NO:1 and SEQ ID NO：2 peptide is used singly or in combination to the activity of anti-wrinkle and includes the cosmetics of the peptide, wherein the cosmetics are included with weight %：SEQ ID NO:1 and/or SEQ ID NO：2 peptide 0.0005 0.001, retinol 0.5 2.5, dried tremella extract 0.5 2.5, purslane extract 2.0 4.0,1,3 propane diols 2.0 4.0, polyglutamic acid 4.0 6.0, rose hip oil 1.5 3.5, soybean lecithin 2.0 4.0, resveratrol 5.0 15.0, carbomer 0.4 0.8, glucan 2.0 4.0, lactobionic acid 1.0 2.0 and niacinamide 1.0 2.0.The cosmetics of the present invention have anti-wrinkle of skin, anti aging effect and other effects.
Description
Technical field
The present invention relates to the non-natural peptide with anti-wrinkle effect and and they make up and personal care composition in
Application.
Background technology
Skin aging is the skin senescence phenomenon as caused by natural cause or non-natural factor.Skin histology day after people's birth
Reach increasingly, function is gradually active, will start to degenerate when reaching certain age, and this degeneration is often unconsciously slow in people
Slow-motion row.The growth stage of skin histology typically ends at 25 years old or so, and it is " bent angle of skin " to have this phase of person, hereafter grows certainly
Carried out simultaneously with aging, Elastic fibers are gradually thicker, and 40~50 the beginning of the year old phase, the aging of skin is slowly obvious, although always
Change degree can vary with each individual.
The infringement collective effects such as skin senescence is the intrinsic physiological mechanism of human senility and the external world exposes to the weather, environmental pollution
Result.Rational nutrition can partly suppress physiological aging, but be difficult to protect skin free from the influence of the external environment.It is only logical
The presenility excessively of skin could be suppressed by crossing supplement nutrient in good time.
Body nutrient balance is critically important to the skin for maintaining health, perhaps skin can not be made to youthen, but ignore this
Point will but greatly speed up skin aging.Known some nutriments lack, such as vitamin C, vitamin B complex and required fat
Acid, various dermatitis and other skin diseases can be caused.If slight lack (such case generally existing but often ignored), may
Clinical symptoms will not be caused, but have impact on the ability of skin healing and renewal.
Skin senescence is generally embodied in skin elasticity, wrinkle of skin and roughness, moistens the several respects such as intensity.Daily life
In it is simple the use of moisturizer skin care is clearly inadequate for prevention skin senescence.The factors such as environment, sunlight and pressure can make
Obtain free radical and greatly injure skin, promote skin ageing to accelerate.At this time skin also easily goes out a little spots, phenomena such as microgroove, institute
To need to use oxidation resistant skin care item.Years cause body ages, and skin metabolism slows down, the collagen and bullet of skin
Property fibre modification.
French patent of invention FR2945939 discloses a kind of new application of Tyr-Arg dipeptides, and it can recover skin
Elasticity, anti-skin sagging, for preventing and/or handling skin aging, wrinkle, striae of pregnancy etc., improve the density of corium and epidermis,
Recover the volume of corium and epidermis.
Japanese invention patent JP2011020951 discloses a kind of preparation for being used to improve cutis laxa, contains pelargonidin
Or eggplant (pelargonidin).
International patent application WO2017022091 disclose it is a kind of improve due to cutis laxa caused by skin cavitation and
The preparation of aging, Rosmarinus officinalis extract and licorice can attract fat stem cell to attract, and thus be caused by the cavitation of corium
Cutis laxa or aging.
Chinese invention patent CN105708732 discloses a kind of composition, comprising in amine, acid amides and polyalcohol extremely
Few one kind is so that as active component, the cosmetic composition has pre- age inhibiting effect.
Chinese invention patent CN105407866 discloses the purposes of the combination comprising collagen and vitamin B3, and the collagen is excellent
Elect the form of marine collagen or its hydrolysis as, the purposes has at least one effect to skin beneficiating, described to have to skin
The effect of benefit is selected from desalination wrinkle, the elastic stage for promoting turgor or skin, improves skin firmness and smoothness, raising
Moisture content of skin and lifting skin gloss.The invention further relates to comprise at least form, the vitamin B3 and one of collagen or its hydrolysis
The combination of the activating agent of kind dried tremella extract and the beauty method of correlation.
Chinese invention patent CN104903346 discloses a kind of synthetic peptide, and the synthetic peptide is by residual with 20-39 amino acid
Base, it has the function that preventing and/or relaxing skin aging.
Chinese invention patent CN104394876 discloses a kind of composition for being used to prevent skin aging, said composition bag
Containing the tea in the fresh tea leaf inactivated from enzyme.
Chinese invention patent CN103957998 discloses a kind of skin anti-aging composition, especially, includes myricetin
Or the skin anti-aging composition of its glucosides and at least one LXR alfa agonists.Skin aging refers to following any one or more of
Outward appearance or performance：Wrinkle or sagging, poor skin barrier such as dry, scalp pruritus or the colour of skin are uneven such as senile plaque expelling.
Chinese invention patent CN103655398 discloses a kind of anti-skin aging face cream, by the original of following parts by weight
Material is made：Rose hip oil 5-10 parts, pearl powder 3-7 parts, deionized water 25-45 parts, Ginseng extract 4-8 parts, vaseline 3-6
Part, radix glycyrrhizae 2-5 parts, xanthans 4-7 parts, glycerine 3-5 parts, sodium hydroxide 2-5 parts, saualane 4-8 parts, hexadecanol 2-6 parts, list is firmly
Glycerol 3-5 parts, stearic acid 4-6 parts, ascorbic acid aliphatic ester 4-8 parts, cluck xanthan 1-3 parts, triethanolamine 2-4 parts.
It can repair deep skin necrosis skin, moisturizing moisturizing, bright color, desalinate wrinkle.
With present growth in the living standard, people especially women also increasingly payes attention to for the external image of oneself,
It is desired at present so being capable of the cosmetics of anti-wrinkle skin.
The content of the invention
To develop the new type of peptides with anti-wrinkle effect, present inventor has performed insistent research and peptide text has been screened
Storehouse.Finally, the inventor has discovered that the new type of peptides with excellent anti-wrinkle effect, finally realizes the purpose of the present invention.
One object of the present invention has the peptide of anti-wrinkle effect to provide.
Another object of the present invention is a kind of composition for anti-wrinkle of offer.
A further object of the present invention is a kind of method for anti-wrinkle of offer.
To realize the purpose of the present invention, the first aspect of the present invention, there is provided peptide, it is selected from following peptide：
(i) peptide, it includes or is made up of following amino acid residue：
Arg-Ala-Ala-Asp-Cys-Gln-Met-Pro-Cys-Leu-Ala(RAADCQMPCLA)(SEQ ID NO:
1)；Or
(ii) peptide, it includes or is made up of following amino acid residue：
Asp-Ala-Cys-Gln-His-Leu-Ala-Pro-Ser-Thr(DACQHLAPST)(SEQ ID NO:2)。
The peptide of the present invention is alternatively referred to as " Peptide.03 peptides ".
According to the preferable aspect of the present invention, there is provided the combination of peptide, it includes SEQ ID NO:1 and SEQ ID NO:2
Peptide combination.
According to the second aspect of the invention, there is provided anti-wrinkle composition, it includes the SEQ ID NO of the present invention:1 peptide
And cosmetically acceptable carrier.
According to the preferable aspect of the present invention, there is provided anti-wrinkle composition, it includes the SEQ ID NO of the present invention:1 He
SEQ ID NO：2 peptide combination and cosmetically acceptable carrier.
According to the third aspect of the invention we, there is provided SEQ ID NO of the invention:1 peptide is used for the purposes of anti-wrinkle.
According to a preferred aspect of the present invention, there is provided SEQ ID NO of the invention:1 and SEQ ID NO：2 peptide combination is used
In the purposes of anti-wrinkle.
The present invention's additionally provides a kind of cosmetics for anti-wrinkle, and it is included：
(a) single peptide or peptide of the invention combination；
(b) antioxidant；
(C) NMF；
(d) vegetable oil；
(e) lecithin；
(f) preservative；
(g) essential oil；With
(h) purified water.
After following detailed description of the invention, including exemplary embodiment and embodiment has been read, this hair
Bright these and other aspects will become obvious for those skilled in the art.
Two peptides are claimed in the present invention, that is, there is SEQ ID NO:1 peptide (i) has SEQ ID NO:2
Peptide (ii).When these peptides are used for anti-wrinkle composition, the suppression provides the benefit in terms of anti-wrinkle.
Additionally by experiment, not only confirm that each self energy of the two peptides reduces sebum secretion, and be also noted that they
Cooperative interaction when being used together.
As used herein, it is intended to that there are them in the general of this area unless otherwise specifically defined all terms
Implication.Term " amino acid " is intended to include naturally occurring amino acid and non-naturally occurring amino acid, broadly including institute
There is the small molecule with a carboxyl and at least one primary or secondary amine groups that can form peptide bond.Term " peptide " is intended to include all tools
There is the molecule of at least one peptide bond, therefore including dipeptides, tripeptides, oligopeptides and the polypeptide for being up to about 20 amino acid residues.
Term " peptide " also include with one or more linkers, interval base or terminal groups, be not the structure of amino acid.
The peptide of the present invention provides in cosmetically acceptable carrier.Carrier can be hydrophobic or hydrophilic.It is suitable
When hydrophobic carrier include for example, commonly using waxy nonionic, such as the ester and ether of fatty alcohol and aliphatic acid, carbon in cosmetics
Chain length is C4 to C22, preferably C8 to C18, most preferably C12 to C18.
The example of fatty hydrophobic carrier includes isopropyl myristate, isopropyl palmitate, octyl palmitate, lanolin fatty acid
Isopropyl ester, acetulan, the benzoic ether of C12-C15 alcohol, Octanoic acid, hexadecyl ester, cetin, myristic acid meat
Cardamom ester, Tetradecyl lactate, acetic acid cetyl, two caprylic/capric propylene glycol esters, decyl oleate, acetylated lanolin, enanthic acid
Stearate, malic acid diisopstearate, octyl hydroxystearate, octyl hydroxystearate, isopropyl isostearate etc..
Appropriate hydrophilic support solution can be, such as the glycol for the alkoxylate commonly used in glycol and cosmetics, including
Ethylene glycol, diethylene glycol, triethylene glycol, propane diols, DPG etc..
Anti-wrinkle composition can be configured to nutrient solution, Essence, cosmetics, emulsifiable paste, toner, slurries, spray, rod
Agent and other forms well known by persons skilled in the art.Emulsifiable paste and toner are currently preferred product forms.
In cosmetically acceptable carrier, the concentration range of peptide can be 1 μM to 200 μM, preferably 10ppb to 150 μM,
More preferably 20 μM to 100 μM, most preferably 36 μM to 50 μM.
Anti-wrinkle composition typically comprises above-mentioned carrier solution, and its level is about 0.01% to about 90% weight, excellent
Select about 0.1% to about 50% weight, more preferably from about 0.1% to about 20% weight, most preferably from about 1% to about 10% weight.
As antioxidant, such as butylated hydroxyanisole (BHA), dibutyl hydroxy toluene, sodium hydrogensulfite, anti-bad can be enumerated
Hematic acid and its salt, glutathione, glutathione peroxidase, glutathione-S-transferase, catalase, superoxides discrimination
Change enzyme, thioredoxin, taurine, thiolaurine, hypotaurine, vitamin E etc..Wherein, preferably ascorbic acid, gluathione
Peptide, vitamin E and taurine.These antioxidants can be used and a kind or two or more are applied in combination, preferably vitamin E and
The combination of taurine.In the composition for external application (such as crease-resistant nutrient solution) of the present invention during addition antioxidant, it is added
Ratio is not particularly limited, but relative to the total amount of composition for external application, usually 0.001~6 weight %, preferably 0.01
~3 weight %, more preferably 0.2~2 weight %.
As NMF, such as alanine, serine, leucine, isoleucine, threonine, glycine, dried meat ammonia can be enumerated
The amino acid such as acid, hydroxy-proline, aspartic acid, arginine, theanine and its derivative；Ethylene glycol, 1,3-PD, 1,3-
The polyalcohols such as butanediol, diethylene glycol (DEG), triethylene glycol, tetraethylene glycol, DPG, 2 1,3 propane diols, polyethylene glycol；Ethylene glycol list first
Base ether, ethylene glycol monomethyl ether, ethylene glycol monopropyl ether, diethylene glycol monomethyl ether, TC, diethylene glycol
Single propyl ether, diethylene glycol monobutyl ether, propylene glycol monoethyl, propylene glycol monopropyl ether, dihydroxypropane single-ethyl ether, dipropyl
The glycol ethers such as glycol list propyl ether；The sugar alcohols such as mannitol, D-sorbite, erythrite, xylitol, trehalose；Lecithin, hydrogenation ovum
The phosphatide such as phosphatide；The mucopolysaccharides such as Sodium Hyaluronate, acetyl group Sodium Hyaluronate, hyparinoids from animal organs, sodium chondroitin sulfate；Gelatine
The high-molecular compounds such as original, elastin laminin, keratin, chitin, chitosan, gelatin, polyglutamic acid；Sodium lactate, pyrrole
The compositions such as pyrrolidone carboxylic acid's sodium；Camomile extract, aloe extract, aloe vera extract, hamamelis extract, fan change
Plant extracts such as fragrant extract, thyme extract, tea extraction, purslane extract etc..Wherein, preferably glycine, 1,3
Propane diols, DPG, 2 1,3 propane diols, TC, Sodium Hyaluronate, acetyl group Sodium Hyaluronate, pyrroles
Alkanone carboxylic acid sodium, polyglutamic acid.These NMFs can use a kind or two or more is applied in combination, preferably 1,3-PD
And polyglutamic acid.In the composition for external application (such as crease-resistant nutrient solution) of the present invention during addition NMF, its adding proportion
It is not particularly limited, but relative to the total amount of composition for external application (such as crease-resistant nutrient solution), usually 0.001~40 weight
Measure %, more preferably preferably 0.1~30 weight %, 5~20 weight %.
As vegetable oil, it is selected from rose hip oil, Sweet Almond Oil, rose oil, jojoba oil, wheat-germ oil, carrot
Oil, Avocado Oil, castor oil, avocado oil, coconut oil, palm oil, cupu oil, cocoa butter, palm-kernel oil, soybean oil, rice bran oil, reed
Luxuriant growth oil, mother-in-law Luo Yue purports, mowrah fat, sher butter, peanut oil, corn oil, macadimia nut oil, candlenut oil, husky thorn oil, flax
Oil, passionflower caul-fat, pot marigold, mango kernel oil, preferably cynarrhodion oil, one kind of Sweet Almond Oil, wheat-germ oil, aloe
Or two or more combination.Preferably, the vegetable oil in composition of the invention is wheat-germ oil.In the skin of the present invention
In topical composition (such as crease-resistant nutrient solution) during addition vegetable oil, its adding proportion is not particularly limited, but relative to skin
The total amount of topical composition (such as crease-resistant nutrient solution), usually 0.001~10 weight %, preferably 0.5~8 weight %, enter
One step is preferably 1.0~8.0 weight %.
As preservative, it is selected from phenmethylol, benzyl carbinol, Phenoxyethanol and lauroyl arginine ethyl ester, preferably bay
Acyl arginine ethyl ester.In the composition for external application (such as crease-resistant nutrient solution) of the present invention during addition preservative, it adds ratio
Example is not particularly limited, but relative to the total amount of composition for external application (such as crease-resistant nutrient solution), usually 0.001~5 weight
Measure %, more preferably preferably 0.1~5 weight %, 0.2~3 weight %.
As lecithin, it is preferably soybean lecithin.In composition for external application (such as the crease-resistant nutrition of the present invention
Liquid) in addition lecithin when, its adding proportion is not particularly limited, but relative to composition for external application (such as crease-resistant nutrition
Liquid) total amount, usually 0.01~5 weight %, preferably 0.1~5 weight %, more preferably 0.2~2 weight %.
Preferably, vitamin E2 .0-4.0, taurine 0.5-2.5, the wheat of toiletry bag % containing weight of the invention meter
Embryo oil 0.5-2.5, purslane extract 2.0-4.0,1,3 propane diols 2.0-4.0, polyglutamic acid 4.0-6.0, avocado oil 1.5-
3.5th, soybean lecithin 2.0-4.0, resveratrol 5.0-12.0, carbomer 0.4-0.8, glucan 2.0-4.0, lactobionic acid 1.0-
2.0 and niacinamide 1.0-2.0.
It is highly preferred that toiletry bag weight containing the 0.0005-0.001 % of present invention SEQ ID NO：1 peptide and/or SEQ
ID NO：2 peptides.It is further preferred that the cosmetics of the present invention also the lauroyl arginine ethyl ester comprising appropriate amount and the water of surplus.
It is further preferred that the cosmetics of the present invention, it is included with weight %：
SEQ ID NO：1 peptide 0.001, retinol 0.5-2.5, dried tremella extract 0.5-2.5, purslane extract 2.0-
4.0th, 1,3 propane diols 2.0-4.0, polyglutamic acid 4.0-6.0, rose hip oil 1.5-3.5, soybean lecithin 2.0-4.0, white black false hellebore
Alcohol 5.0-15.0, carbomer 0.4-0.8, glucan 2.0-4.0, lactobionic acid 1.0-2.0 and niacinamide 1.0-2.0, surplus are
Water.
Further, cosmetics of the invention, it is included with weight %：
SEQ ID NO：2 peptide 0.001, retinol 0.5-2.5, dried tremella extract 0.5-2.5, purslane extract 2.0-
4.0th, 1,3 propane diols 2.0-4.0, polyglutamic acid 4.0-6.0, rose hip oil 1.5-3.5, soybean lecithin 2.0-4.0, white black false hellebore
Alcohol 5.0-15.0, carbomer 0.4-0.8, glucan 2.0-4.0, lactobionic acid 1.0-2.0 and niacinamide 1.0-2.0, surplus are
Water.
Yet further, cosmetics of the invention, it is included with weight %：
SEQ ID NO：1 peptide 0.0005, SEQ ID NO：2 peptide 0.0005, retinol 0.5-2.5, dried tremella extract
0.5-2.5, purslane extract 2.0-4.0,1,3 propane diols 2.0-4.0, polyglutamic acid 4.0-6.0, rose hip oil 1.5-3.5,
Soybean lecithin 2.0-4.0, resveratrol 5.0-15.0, carbomer 0.4-0.8, glucan 2.0-4.0, lactobionic acid 1.0-2.0,
With niacinamide 1.0-2.0, surplus is water.
As it is used herein, "comprising" and " comprising ", " containing " or " being characterised by " it is synonymous, and in being included in
Or open, and it is not excluded for the other element do not stated or method and step.Term "comprising" any table herein
State, particularly in the method for the description present invention, purposes or during product, it is thus understood that including substantially by the component or element or
Those products, method and the purposes that step is formed and is made up of the component or element or step.The sheet of description exemplified here
Invention suitably can be in the situation in the absence of any one or more of element not specifically disclosed herein, one or more limitations
Under put into practice.
The specification for being related to the anti-wrinkle product included in the anti-wrinkle product of the present invention can contain following content：
Effect and effect (such as anti-wrinkle), application process (such as be uniformly applied to skin, such as face and neck) and it may produce
Raw side effect etc..
The term and statement used herein is used as descriptively rather than restrictive term, and in such a term and statement
Use in it is not expected exclude shown in and the feature or part thereof any equivalent, it is appreciated that various modifications are being asked
It is possible in the scope of the present invention of protection.It is therefore understood that although the present invention by preferred embodiment and optionally
Feature specifically discloses, but those skilled in the art can use the modification and transformation of concept disclosed herein, and such modification
It is considered as with change in the scope of the present invention such as defined by accessory claim.
To be illustrated more clearly that the present invention, it is described in detail in conjunction with following examples, but these embodiments are only
To the exemplary description of the present invention, the limitation to the application should not be construed as.
Embodiment
The following examples are to be used for the explanation present invention in a non limiting manner.
Embodiment 1：Influence to I-type collagen synthesis
By human skin fibroblasts on 48 orifice plates with 1 × 105Cell/ml density plant division, cultivate one day so that thin
Born of the same parents' fusion reaches about 60%, continues culture one day on without FBS culture mediums.Prepare the DMEM culture mediums without FBS, be divided into A, B, C,
Tetra- groups of D, A groups plus deionized water, B groups plus SEQ ID NO:1 peptide make it that its concentration is 10ppm, C groups plus SEQ ID NO:2
Peptide make it that its concentration is 10ppm, and D groups add SEQ ID NO respectively:1 and SEQ ID NO:2 peptide make it that their concentration is respectively
5ppm.The human skin fibroblasts of culture are taken, are washed with phosphate buffer, are moved on in above-mentioned four groups of culture mediums, in CO2Training
Support and cultivated 48 hours in case.To evaluate the expression of precollagen, the nutrient solution that above-mentioned cultural method obtains I procollagen types will be passed through
C- peptide reagents box (Procollagen TypeI C-Peptide (PIP) EIA Kit, Cat#.MK101, Takara, Japan) enters
Quantitative analysis is gone.In 96 orifice plates of the coating that kit provides, the anti-collagen of primary antibody will be used as by the use of supernatant
After antibody is handled 90 minutes at 37 DEG C, washed 3 times with TPBS (0.1%Tween 20in PBS), and secondary antibody will be used as
Anti- mouse IgG (Whole mouse, alkaline phosphatase conjugated) react 90 minutes after, washed with TPBS
3 times.Alkaline phosphatase matrix solution (1mg/mL p-nitrophenyls phosphoric acid-diethanolamine buffer) is reacted 30 points at room temperature
Zhong Hou, the absorbance under 405nm wavelength is determined with ELIASA.
Table 1
| Group | Sample | PIP(ng/ml) |
| A | Control group | 100 |
| B | SEQ ID NO:1 peptide 10ppm | 120 |
| C | SEQ ID NO:2 peptide 10ppm | 116 |
| D | SEQ ID NO:1 peptide 5ppm+SEQ ID NO:2 peptide 5ppm | 141 |
It can be seen that according to data above, compared with blank control, of the invention two kinds of peptides can dramatically increase type i collagen egg
White synthesis, and two kinds of peptides obtain the effect for being considerably better than single peptide in the case of total protein concentration identical, illustrate this two
Kind peptide has synergy in terms of I-type collagen synthesis is promoted.
Embodiment 2：The preparation of crease-resistant nutrient solution
| Composition | Content (weight %) |
| Peptide (the SEQ ID NO of the present invention：1) | 0.001 |
| Retinol | 1.5 |
| Dried tremella extract | 1.5 |
| Purslane extract | 3.0 |
| 1,3 propane diols | 3.0 |
| Polyglutamic acid | 5.0 |
| Rose hip oil | 2.5 |
| Soybean lecithin | 3.0 |
| Resveratrol | 10.0 |
| Carbomer | 0.6 |
| Glucan | 3.0 |
| Lactobionic acid | 1.0 |
| Niacinamide | 1.0 |
| Purified water | Residual volume |
| Amount to | 100 |
Carbomer is dissolved in purified water and adds 1,3 propane diols, stirs and adjusts after being stirring evenly and then adding into other components
Crease-resistant nutrient solution is made.
Embodiment 3：The preparation of crease-resistant nutrient solution
| Composition | Content (weight %) |
| Peptide (the SEQ ID NO of the present invention：2) | 0.001 |
| Retinol | 0.5 |
| Dried tremella extract | 0.5 |
| Purslane extract | 2.0 |
| 1,3 propane diols | 2.0 |
| Polyglutamic acid | 3.0 |
| Rose hip oil | 1.5 |
| Soybean lecithin | 2.0 |
| Resveratrol | 5.0 |
| Carbomer | 0.4 |
| Glucan | 2.0 |
| Lactobionic acid | 2.0 |
| Niacinamide | 2.0 |
| Purified water | Residual volume |
| Amount to | 100 |
Carbomer is dissolved in purified water and adds 1,3 propane diols, stirs and adjusts after being stirring evenly and then adding into other components
Crease-resistant nutrient solution is made.
Embodiment 4：The preparation of crease-resistant nutrient solution
Carbomer is dissolved in purified water and adds 1,3 propane diols, stirs and adjusts after being stirring evenly and then adding into other components
Crease-resistant nutrient solution is made.
Embodiment 5
Skin-tightening efficacy test：
Volunteer:30 people (30 to 50 years old ages)
Packet：30 people are randomly divided into 3 groups, every group of 10 people, everyone gives two kinds of test samples, and one of which is commercially available control
Product, another kind are respectively the crease-resistant nutrient solution prepared in embodiment 2 to 4；
Using:Crease-resistant nutrient solution more than appropriate is taken uniformly to be applied to left face, the right face after cleaning face sooner or later daily
Smear the reference substance without active component.
Cycle:28 days
Determining instrument:German CK MPA580 elasticity testers, U.S.'s CANFIELD companies VISIA skin analysis testers
Volunteer recorded everyone reality using the 28th day with MPA580 elasticity testers, VISIA skin analysis testers
Test data.Experimental data such as table 2 below：
Table 2
As can be seen from the above table, the crease-resistant nutrient solution of embodiment 2,3,4, has obvious anti-wrinkle effect, it was demonstrated that this hair
Bright peptide is crease-resistant to skin certain actual efficacy.But after using the crease-resistant nutrient solution four weeks of embodiment 4, skin elasticity and wrinkle
The improvement situation of line depth is superior to embodiment 2 and 3, shows the anti-wrinkle effect of obvious Synergistic.
By the description of above-mentioned preferred embodiment, the present invention is described it should be appreciated that skill
Art personnel can make a change and change to these embodiments, without departing from of the invention as expressed by following claims
Spirit or scope.
Sequence table
<110>Thousand auspicious bio tech ltd of Wenzhou
<120>The peptide of anti-wrinkle and the cosmetics for including it
<130> 2
<160> 2
<170> PatentIn version 3.5
<210> 1
<211> 11
<212> PRT
<213>Artificial sequence
<220>
<223> sq1
<400> 1
Arg Ala Ala Asp Cys Gln Met Pro Cys Leu Ala
1 5 10
<210> 2
<211> 10
<212> PRT
<213>Artificial sequence
<220>
<223> sq2
<400> 2
Asp Ala Cys Gln His Leu Ala Pro Ser Thr
1 5 10
Claims (10)
1. peptide, it is referred to as " Peptide.03 peptides ", and it has SEQ ID NO：1 amino acid sequence.
2. peptide according to claim 1, it is by SEQ ID NO：1 amino acid sequence composition.
3. the combination of peptide, it includes SEQ ID NO：1 peptide and SEQ ID NO：2 peptide.
4. for the composition of anti-wrinkle, it includes the peptide of claim 1 or 2, or the combination of the peptide comprising claim 3.
5. purposes of the peptide of claim 1 or 2 in the composition for anti-wrinkle is prepared.
6. purposes according to claim 5, wherein the composition is the form of cosmetics.
7. the cosmetics with anti-wrinkle effect, it is included：SEQ ID NO：1 and SEQ ID NO：One or both of 2 peptide, regard
Flavol, dried tremella extract, purslane extract, 1,3 propane diols, polyglutamic acid, rose hip oil, soybean lecithin, resveratrol,
Carbomer, glucan, lactobionic acid and niacinamide.
8. cosmetics according to claim 7, it is included with weight %：
SEQ ID NO：1 peptide 0.001, retinol 0.5-2.5, dried tremella extract 0.5-2.5, purslane extract 2.0-4.0,
1,3 propane diols 2.0-4.0, polyglutamic acid 4.0-6.0, rose hip oil 1.5-3.5, soybean lecithin 2.0-4.0, resveratrol
5.0-15.0, carbomer 0.4-0.8 and glucan 2.0-4.0, lactobionic acid 1.0-2.0 and niacinamide 1.0-2.0, surplus are
Water.
9. cosmetics according to claim 7, it is included with weight %：
SEQ ID NO：2 peptide 0.001, retinol 0.5-2.5, dried tremella extract 0.5-2.5, purslane extract 2.0-4.0,
1,3 propane diols 2.0-4.0, polyglutamic acid 4.0-6.0, rose hip oil 1.5-3.5, soybean lecithin 2.0-4.0, resveratrol
5.0-15.0, carbomer 0.4-0.8 and glucan 2.0-4.0, lactobionic acid 1.0-2.0 and niacinamide 1.0-2.0, surplus are
Water.
10. cosmetics according to claim 7, it is included with weight %：
SEQ ID NO：1 peptide 0.0005, SEQ ID NO：2 peptide 0.0005, retinol 0.5-2.5, dried tremella extract 0.5-
2.5th, purslane extract 2.0-4.0,1,3 propane diols 2.0-4.0, polyglutamic acid 4.0-6.0, rose hip oil 1.5-3.5, soybean
Lecithin 2.0-4.0, resveratrol 5.0-15.0, carbomer 0.4-0.8 and glucan 2.0-4.0, lactobionic acid 1.0-2.0 and
Niacinamide 1.0-2.0, surplus are water.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710665855.XA CN107365355B (en) | 2017-08-07 | 2017-08-07 | Anti-wrinkle peptide and cosmetic comprising same |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710665855.XA CN107365355B (en) | 2017-08-07 | 2017-08-07 | Anti-wrinkle peptide and cosmetic comprising same |
Publications (2)
| Publication Number | Publication Date |
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| CN107365355A true CN107365355A (en) | 2017-11-21 |
| CN107365355B CN107365355B (en) | 2020-06-26 |
Family
ID=60309871
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710665855.XA Active CN107365355B (en) | 2017-08-07 | 2017-08-07 | Anti-wrinkle peptide and cosmetic comprising same |
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| Country | Link |
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| CN (1) | CN107365355B (en) |
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| JP2019210216A (en) * | 2018-05-31 | 2019-12-12 | 株式会社コーセー | Skin external preparation or cosmetics |
| CN113057932A (en) * | 2021-03-19 | 2021-07-02 | 李雪云 | Collagen skin care product for improving skin wrinkles |
| CN114560912A (en) * | 2022-03-28 | 2022-05-31 | 碧悦星泽(北京)生物医药科技有限公司 | Polypeptide with anti-wrinkle function and compound and application thereof |
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|---|---|
| CN107365355B (en) | 2020-06-26 |
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