CN102976848B - Dendrobium officinale culture solution - Google Patents
Dendrobium officinale culture solution Download PDFInfo
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- CN102976848B CN102976848B CN201210502454.XA CN201210502454A CN102976848B CN 102976848 B CN102976848 B CN 102976848B CN 201210502454 A CN201210502454 A CN 201210502454A CN 102976848 B CN102976848 B CN 102976848B
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- sulfate
- vitamin
- dendrobium officinale
- herba dendrobii
- nutrient solution
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- 241001076416 Dendrobium tosaense Species 0.000 title abstract description 9
- NLKNQRATVPKPDG-UHFFFAOYSA-M potassium iodide Chemical compound [K+].[I-] NLKNQRATVPKPDG-UHFFFAOYSA-M 0.000 claims abstract description 42
- 229940088594 vitamin Drugs 0.000 claims abstract description 42
- 235000013343 vitamin Nutrition 0.000 claims abstract description 42
- 239000011782 vitamin Substances 0.000 claims abstract description 42
- 229930003231 vitamin Natural products 0.000 claims abstract description 42
- 150000003722 vitamin derivatives Chemical class 0.000 claims abstract description 42
- DHMQDGOQFOQNFH-UHFFFAOYSA-N Glycine Chemical compound NCC(O)=O DHMQDGOQFOQNFH-UHFFFAOYSA-N 0.000 claims abstract description 28
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 claims abstract description 28
- FGIUAXJPYTZDNR-UHFFFAOYSA-N potassium nitrate Chemical compound [K+].[O-][N+]([O-])=O FGIUAXJPYTZDNR-UHFFFAOYSA-N 0.000 claims abstract description 28
- 235000018290 Musa x paradisiaca Nutrition 0.000 claims abstract description 16
- 235000013575 mashed potatoes Nutrition 0.000 claims abstract description 15
- PRPINYUDVPFIRX-UHFFFAOYSA-N 1-naphthaleneacetic acid Chemical compound C1=CC=C2C(CC(=O)O)=CC=CC2=C1 PRPINYUDVPFIRX-UHFFFAOYSA-N 0.000 claims abstract description 14
- PAWQVTBBRAZDMG-UHFFFAOYSA-N 2-(3-bromo-2-fluorophenyl)acetic acid Chemical compound OC(=O)CC1=CC=CC(Br)=C1F PAWQVTBBRAZDMG-UHFFFAOYSA-N 0.000 claims abstract description 14
- 229920001817 Agar Polymers 0.000 claims abstract description 14
- UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 claims abstract description 14
- ZGTMUACCHSMWAC-UHFFFAOYSA-L EDTA disodium salt (anhydrous) Chemical compound [Na+].[Na+].OC(=O)CN(CC([O-])=O)CCN(CC(O)=O)CC([O-])=O ZGTMUACCHSMWAC-UHFFFAOYSA-L 0.000 claims abstract description 14
- 239000004471 Glycine Substances 0.000 claims abstract description 14
- SQUHHTBVTRBESD-UHFFFAOYSA-N Hexa-Ac-myo-Inositol Natural products CC(=O)OC1C(OC(C)=O)C(OC(C)=O)C(OC(C)=O)C(OC(C)=O)C1OC(C)=O SQUHHTBVTRBESD-UHFFFAOYSA-N 0.000 claims abstract description 14
- 229930006000 Sucrose Natural products 0.000 claims abstract description 14
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 claims abstract description 14
- 239000008272 agar Substances 0.000 claims abstract description 14
- KGBXLFKZBHKPEV-UHFFFAOYSA-N boric acid Chemical compound OB(O)O KGBXLFKZBHKPEV-UHFFFAOYSA-N 0.000 claims abstract description 14
- 239000004327 boric acid Substances 0.000 claims abstract description 14
- 239000001110 calcium chloride Substances 0.000 claims abstract description 14
- 229910001628 calcium chloride Inorganic materials 0.000 claims abstract description 14
- GVPFVAHMJGGAJG-UHFFFAOYSA-L cobalt dichloride Chemical compound [Cl-].[Cl-].[Co+2] GVPFVAHMJGGAJG-UHFFFAOYSA-L 0.000 claims abstract description 14
- 229910000365 copper sulfate Inorganic materials 0.000 claims abstract description 14
- ARUVKPQLZAKDPS-UHFFFAOYSA-L copper(II) sulfate Chemical compound [Cu+2].[O-][S+2]([O-])([O-])[O-] ARUVKPQLZAKDPS-UHFFFAOYSA-L 0.000 claims abstract description 14
- 239000011790 ferrous sulphate Substances 0.000 claims abstract description 14
- 235000003891 ferrous sulphate Nutrition 0.000 claims abstract description 14
- 229960000367 inositol Drugs 0.000 claims abstract description 14
- CDAISMWEOUEBRE-GPIVLXJGSA-N inositol Chemical compound O[C@H]1[C@H](O)[C@@H](O)[C@H](O)[C@H](O)[C@@H]1O CDAISMWEOUEBRE-GPIVLXJGSA-N 0.000 claims abstract description 14
- BAUYGSIQEAFULO-UHFFFAOYSA-L iron(2+) sulfate (anhydrous) Chemical compound [Fe+2].[O-]S([O-])(=O)=O BAUYGSIQEAFULO-UHFFFAOYSA-L 0.000 claims abstract description 14
- 229910000359 iron(II) sulfate Inorganic materials 0.000 claims abstract description 14
- 229910052943 magnesium sulfate Inorganic materials 0.000 claims abstract description 14
- 235000019341 magnesium sulphate Nutrition 0.000 claims abstract description 14
- SQQMAOCOWKFBNP-UHFFFAOYSA-L manganese(II) sulfate Chemical compound [Mn+2].[O-]S([O-])(=O)=O SQQMAOCOWKFBNP-UHFFFAOYSA-L 0.000 claims abstract description 14
- 235000007715 potassium iodide Nutrition 0.000 claims abstract description 14
- 239000004323 potassium nitrate Substances 0.000 claims abstract description 14
- 235000010333 potassium nitrate Nutrition 0.000 claims abstract description 14
- CDAISMWEOUEBRE-UHFFFAOYSA-N scyllo-inosotol Natural products OC1C(O)C(O)C(O)C(O)C1O CDAISMWEOUEBRE-UHFFFAOYSA-N 0.000 claims abstract description 14
- TVXXNOYZHKPKGW-UHFFFAOYSA-N sodium molybdate (anhydrous) Chemical compound [Na+].[Na+].[O-][Mo]([O-])(=O)=O TVXXNOYZHKPKGW-UHFFFAOYSA-N 0.000 claims abstract description 14
- NWONKYPBYAMBJT-UHFFFAOYSA-L zinc sulfate Chemical compound [Zn+2].[O-]S([O-])(=O)=O NWONKYPBYAMBJT-UHFFFAOYSA-L 0.000 claims abstract description 14
- 229910000368 zinc sulfate Inorganic materials 0.000 claims abstract description 14
- 229960001763 zinc sulfate Drugs 0.000 claims abstract description 14
- 235000015097 nutrients Nutrition 0.000 claims description 26
- 241000234295 Musa Species 0.000 claims description 15
- 239000003109 Disodium ethylene diamine tetraacetate Substances 0.000 claims description 13
- 229910019142 PO4 Inorganic materials 0.000 claims description 13
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 claims description 13
- 235000019301 disodium ethylene diamine tetraacetate Nutrition 0.000 claims description 13
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 claims description 13
- 239000010452 phosphate Substances 0.000 claims description 13
- 239000011591 potassium Substances 0.000 claims description 13
- 229910052700 potassium Inorganic materials 0.000 claims description 13
- 229960004839 potassium iodide Drugs 0.000 claims description 13
- 239000000843 powder Substances 0.000 claims description 13
- XYFCBTPGUUZFHI-UHFFFAOYSA-N Phosphine Chemical compound P XYFCBTPGUUZFHI-UHFFFAOYSA-N 0.000 claims description 12
- RMOGWMIKYWRTKW-UONOGXRCSA-N (S,S)-paclobutrazol Chemical compound C([C@@H]([C@@H](O)C(C)(C)C)N1N=CN=C1)C1=CC=C(Cl)C=C1 RMOGWMIKYWRTKW-UONOGXRCSA-N 0.000 claims description 6
- IXVMHGVQKLDRKH-VRESXRICSA-N Brassinolide Natural products O=C1OC[C@@H]2[C@@H]3[C@@](C)([C@H]([C@@H]([C@@H](O)[C@H](O)[C@H](C(C)C)C)C)CC3)CC[C@@H]2[C@]2(C)[C@@H]1C[C@H](O)[C@H](O)C2 IXVMHGVQKLDRKH-VRESXRICSA-N 0.000 claims description 6
- NWBJYWHLCVSVIJ-UHFFFAOYSA-N N-benzyladenine Chemical compound N=1C=NC=2NC=NC=2C=1NCC1=CC=CC=C1 NWBJYWHLCVSVIJ-UHFFFAOYSA-N 0.000 claims description 6
- 239000005985 Paclobutrazol Substances 0.000 claims description 6
- IXVMHGVQKLDRKH-KNBKMWSGSA-N brassinolide Chemical compound C1OC(=O)[C@H]2C[C@H](O)[C@H](O)C[C@]2(C)[C@H]2CC[C@]3(C)[C@@H]([C@H](C)[C@@H](O)[C@H](O)[C@@H](C)C(C)C)CC[C@H]3[C@@H]21 IXVMHGVQKLDRKH-KNBKMWSGSA-N 0.000 claims description 6
- 235000009508 confectionery Nutrition 0.000 claims description 6
- 229910000073 phosphorus hydride Inorganic materials 0.000 claims description 6
- 241000196324 Embryophyta Species 0.000 abstract description 4
- 150000004676 glycans Chemical class 0.000 abstract description 4
- 229920001282 polysaccharide Polymers 0.000 abstract description 4
- 239000005017 polysaccharide Substances 0.000 abstract description 4
- 230000004083 survival effect Effects 0.000 abstract description 3
- 150000001413 amino acids Chemical class 0.000 abstract description 2
- 240000008790 Musa x paradisiaca Species 0.000 abstract 1
- 235000010338 boric acid Nutrition 0.000 abstract 1
- 238000012258 culturing Methods 0.000 abstract 1
- 239000004615 ingredient Substances 0.000 abstract 1
- 229960003390 magnesium sulfate Drugs 0.000 abstract 1
- 229940099596 manganese sulfate Drugs 0.000 abstract 1
- 239000011702 manganese sulphate Substances 0.000 abstract 1
- 235000007079 manganese sulphate Nutrition 0.000 abstract 1
- 229910000402 monopotassium phosphate Inorganic materials 0.000 abstract 1
- 235000019796 monopotassium phosphate Nutrition 0.000 abstract 1
- 235000016709 nutrition Nutrition 0.000 abstract 1
- 230000035764 nutrition Effects 0.000 abstract 1
- GNSKLFRGEWLPPA-UHFFFAOYSA-M potassium dihydrogen phosphate Chemical compound [K+].OP(O)([O-])=O GNSKLFRGEWLPPA-UHFFFAOYSA-M 0.000 abstract 1
- LWIHDJKSTIGBAC-UHFFFAOYSA-K potassium phosphate Substances [K+].[K+].[K+].[O-]P([O-])([O-])=O LWIHDJKSTIGBAC-UHFFFAOYSA-K 0.000 abstract 1
- 239000011684 sodium molybdate Substances 0.000 abstract 1
- 235000015393 sodium molybdate Nutrition 0.000 abstract 1
- 239000012153 distilled water Substances 0.000 description 5
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 5
- 240000004638 Dendrobium nobile Species 0.000 description 3
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 2
- 230000003321 amplification Effects 0.000 description 2
- 239000003814 drug Substances 0.000 description 2
- 238000005286 illumination Methods 0.000 description 2
- 238000003199 nucleic acid amplification method Methods 0.000 description 2
- 239000007787 solid Substances 0.000 description 2
- 235000015961 tonic Nutrition 0.000 description 2
- 230000001256 tonic effect Effects 0.000 description 2
- 244000025254 Cannabis sativa Species 0.000 description 1
- 241000233855 Orchidaceae Species 0.000 description 1
- 244000061456 Solanum tuberosum Species 0.000 description 1
- 235000002595 Solanum tuberosum Nutrition 0.000 description 1
- 240000001519 Verbena officinalis Species 0.000 description 1
- 235000018718 Verbena officinalis Nutrition 0.000 description 1
- 235000001014 amino acid Nutrition 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 210000002615 epidermis Anatomy 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 229910052742 iron Inorganic materials 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 238000000034 method Methods 0.000 description 1
- 230000000050 nutritive effect Effects 0.000 description 1
- 230000003014 reinforcing effect Effects 0.000 description 1
- 239000011435 rock Substances 0.000 description 1
- 230000008653 root damage Effects 0.000 description 1
- 210000002784 stomach Anatomy 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 229960000716 tonics Drugs 0.000 description 1
Abstract
The invention discloses a Dendrobium officinale culture solution and belongs to the technical field of agricultural planting. The culture solution comprises the following ingredients: potassium nitrate, ammonium nitrate, magnesium sulfate, manganese sulfate, zinc sulfate, monopotassium phosphate, copper sulfate, potassium iodide, cobalt dichloride, boric acid, sodium molybdate, vitamin VB1, vitamin VB6, vitamin VB5, glycine, ferrous sulfate, disodium ethylenediamine tetraacetic acid, calcium chloride, naphthylacetic acid, mashed banana, mashed potato, white sugar, inositol, and powdered agar. The dendrobium officinale culture solution is reasonable in compatibility, can provide comprehensive nutrition for a Dendrobium officinale stem, fast promote the stem to grow out of a root, increase the polysaccharide content and the amino acid content in the Dendrobium officinale, shorten the time for culturing the Dendrobium officinale stem to a complete plant to about 25 days, and significantly improve the transplanting survival rate of the Dendrobium officinale.
Description
Technical field
The invention belongs to agricultural planting technique field, be specifically related to a kind of Herba Dendrobii nutrient solution.
Background technology
Herba Dendrobii, herbaceous plant grows nonparasitically upon another plant for the orchid family is perennial.Be born in height above sea level and reach on the rock that the mountain region half of 1600 meters is dark and damp, the environment of happiness warm and moist weather and half cloudy half light, cold-resistant.The low temperature of general equal ability-5 DEG C.The stem of noble dendrobium can be divided into tens of kinds, yellow grass, Jin Chai, horsewhip etc., the superfine product that Herba Dendrobii is the stem of noble dendrobium, and because epidermis is, iron is green gains the name for it.Herba Dendrobii has unique pharmaceutical use, is used as medicine with its stem, and Chinese medicine name: the stem of noble dendrobium, belongs to the yin tonics in tonic, " Chinese Pharmacopoeia ": reinforcing stomach reg fluid is nourishing Yin and clearing heat.
At present, in the breeding system of dendrobium officinale test-tube plantlet, what root media adopted is solid medium, and the application of solid medium, has not only increased production cost, and in the time getting seedling, easily causes root injury, has reduced transplanting survival rate.
Summary of the invention
The problem existing for prior art, the object of the invention is to design provides a kind of technical scheme of Herba Dendrobii nutrient solution.
Described a kind of Herba Dendrobii nutrient solution, is characterized in that being made up of following component:
Saltpetre 47～57g/L, ammonium nitrate 40～50g/L, magnesium sulfate 10.4～20.4g/L, manganous sulfate 3.2～7.2 g/L, zinc sulfate 0.5～1.5g/L, potassium primary phosphate 1.5～2.5g/L, copper sulfate 0.7～1.7g/L, potassiumiodide 0.9～1.9 g/L, cobalt chloride 0.15～0.55g/L, boric acid 5.8～9.8g/L, Sodium orthomolybdate 0.37～0.77g/L, vitamin V B1 0.6～1.0g/L, vitamin V B6 0.25～0.65g/L, vitamin V B5 0.33～0.83g/L, glycine 0.61～1.01g/L, ferrous sulfate 0.58～0.98 g/L, disodium ethylene diamine tetraacetate 1.3～1.7g/L, calcium chloride 1.3～3.3g/L, naphthylacetic acid 0.13～0.53g/L, banana puree 10～20g/L, mashed potato 19～29g/L, white sugar 6～16g/L, inositol 46～56g/L, agar powder 1.0～2.0g/L.
Described a kind of Herba Dendrobii nutrient solution, is characterized in that being made up of following component:
Saltpetre 49～55g/L, ammonium nitrate 42～48g/L, magnesium sulfate 12.4～18.4g/L, manganous sulfate 4.2～6.2 g/L, zinc sulfate 0.7～1.3g/L, potassium primary phosphate 1.7～2.3g/L, copper sulfate 0.9～1.5g/L, potassiumiodide 1.1～1.7 g/L, cobalt chloride 0.25～0.45g/L, boric acid 6.8～8.8g/L, Sodium orthomolybdate 0.47～0.67g/L, vitamin V B1 0.7～0.9g/L, vitamin V B6 0.3～0.6g/L, vitamin V B5 0.4～0.8g/L, glycine 0.7～1.0g/L, ferrous sulfate 0.6～0.9 g/L, disodium ethylene diamine tetraacetate 1.4～1.6g/L, calcium chloride 1.5～3.0g/L, naphthylacetic acid 0.2～0.5g/L, banana puree 12～18g/L, mashed potato 20～28g/L, white sugar 8～14g/L, inositol 48～54g/L, agar powder 1.2～1.8g/L.
Described a kind of Herba Dendrobii nutrient solution, is characterized in that being made up of following component:
Saltpetre 50～54g/L, ammonium nitrate 44～46g/L, magnesium sulfate 13～17g/L, manganous sulfate 5～6g/L, zinc sulfate 0.8～1.2g/L, potassium primary phosphate 1.8～2.2g/L, copper sulfate 1.0～1.4g/L, potassiumiodide 1.2～1.5g/L, cobalt chloride 0.3～0.4g/L, boric acid 7～8g/L, Sodium orthomolybdate 0.5～0.6g/L, vitamin V B1 0.75～0.85g/L, vitamin V B6 0.35～0.55g/L, vitamin V B5 0.5～0.7g/L, glycine 0.75～0.95g/L, ferrous sulfate 0.65～0.85g/L, disodium ethylene diamine tetraacetate 1.45～1.55g/L, calcium chloride 1.8～2.5g/L, naphthylacetic acid 0.25～0.45g/L, banana puree 14～16g/L, mashed potato 22～26g/L, white sugar 10～12g/L, inositol 50～52g/L, agar powder 1.4～1.6g/L.
Described a kind of Herba Dendrobii nutrient solution, is characterized in that being made up of following component:
Saltpetre 51～53g/L, ammonium nitrate 44.5～45.5g/L, magnesium sulfate 14～16g/L, manganous sulfate 5.2～5.5g/L, zinc sulfate 1.0～1.2g/L, potassium primary phosphate 1.9～2.1g/L, copper sulfate 1.1～1.3g/L, potassiumiodide 1.3～1.4g/L, cobalt chloride 0.32～0.38g/L, boric acid 7.2～7.8g/L, Sodium orthomolybdate 0.52～0.58g/L, vitamin V B1 0.78～0.82g/L, vitamin V B6 0.45～0.52g/L, vitamin V B5 0.55～0.65g/L, glycine 0.8～0.86g/L, ferrous sulfate 0.7～0.8g/L, disodium ethylene diamine tetraacetate 1.48～1.52g/L, calcium chloride 2.0～2.2g/L, naphthylacetic acid 0.3～0.4g/L, banana puree 14.5～15.5g/L, mashed potato 24～25g/L, white sugar 11～12g/L, inositol 51～52g/L, agar powder 1.45～1.55g/L.
Described a kind of Herba Dendrobii nutrient solution, is characterized in that also containing 6-benzylaminopurine 0.1～0.6g/L, brassinolide 0.2～0.8g/L, paclobutrazol 0.35～0.65g/L and increases sweet phosphine 0.1～0.6g/L.
Described a kind of Herba Dendrobii nutrient solution, is characterized in that also containing 6-benzylaminopurine 0.2～0.4g/L, brassinolide 0.3～0.6g/L, paclobutrazol 0.4～0.5g/L and increases sweet phosphine 0.3～0.5g/L.
Above-mentioned a kind of Herba Dendrobii nutrient solution, compatibility is reasonable, can provide comprehensive nutritive substance for Herba Dendrobii stem section, can urge fast the raw stem of root, can improve polysaccharide content in Herba Dendrobii, aminoacids content, shorten Herba Dendrobii stem section to about the incubation time to 25 of whole plant day, significantly improve Herba Dendrobii and transplant survival rate.
Embodiment
Further illustrate the present invention below in conjunction with specific embodiment.In the present invention, banana puree and mashed potato are made up of fresh banana and potato respectively.
Formula one
A kind of Herba Dendrobii nutrient solution, it contains following component: saltpetre 52g/L, ammonium nitrate 45g/L, magnesium sulfate 15.4g/L, manganous sulfate 5.2 g/L, zinc sulfate 1g/L, potassium primary phosphate 2g/L, copper sulfate 1.2g/L, potassiumiodide 1.4g/L, cobalt chloride 0.35g/L, boric acid 7.8g/L, Sodium orthomolybdate 0.57g/L, vitamin V B1 0.8g/L, vitamin V B6 0.45g/L, vitamin V B5 0.53g/L, glycine 0.81g/L, ferrous sulfate 0.789g/L, disodium ethylene diamine tetraacetate 1.588g/L, calcium chloride 2.3g/L, naphthylacetic acid 3.3g/L, banana puree 15g/L, mashed potato 24g/L, white sugar 11g/L, inositol 51g/L, agar powder 1.5g/L, then be settled to 1L with distilled water.
Formula two
A kind of Herba Dendrobii nutrient solution, it contains following component: saltpetre 47g/L, ammonium nitrate 40g/L, magnesium sulfate 10.4g/L, manganous sulfate 3.2g/L, zinc sulfate 0.5g/L, potassium primary phosphate 1.5g/L, copper sulfate 0.7g/L, potassiumiodide 0.9 g/L, cobalt chloride 0.15g/L, boric acid 5.8g/L, Sodium orthomolybdate 0.37g/L, vitamin V B1 0.6g/L, vitamin V B6 0.25g/L, vitamin V B5 0.33g/L, glycine 0.61g/L, ferrous sulfate 0.58g/L, disodium ethylene diamine tetraacetate 1.3g/L, calcium chloride 1.3g/L, naphthylacetic acid 0.13g/L, banana puree 10g/L, mashed potato 19g/L, white sugar 6g/L, inositol 46g/L, agar powder 1.0g/L, then be settled to 1L with distilled water.
Formula three
A kind of Herba Dendrobii nutrient solution, it contains following component: saltpetre 57g/L, ammonium nitrate 50g/L, magnesium sulfate 20.4g/L, manganous sulfate 7.2 g/L, zinc sulfate 1.5g/L, potassium primary phosphate 2.5g/L, copper sulfate 1.7g/L, potassiumiodide 1.9 g/L, cobalt chloride 0.55g/L, boric acid 9.8g/L, Sodium orthomolybdate 0.77g/L, vitamin V B1 1.0g/L, vitamin V B6 0.65g/L, vitamin V B5 0.83g/L, glycine 1.01g/L, ferrous sulfate 0.98 g/L, disodium ethylene diamine tetraacetate 1.7g/L, calcium chloride 3.3g/L, naphthylacetic acid 0.53g/L, banana puree 20g/L, mashed potato 29g/L, white sugar 16g/L, inositol 56g/L, agar powder 2.0g/L, then be settled to 1L with distilled water.
Formula four
A kind of Herba Dendrobii nutrient solution, it contains following component: saltpetre 49g/L, ammonium nitrate 42g/L, magnesium sulfate 12.4g/L, manganous sulfate 4.2g/L, zinc sulfate 0.7g/L, potassium primary phosphate 1.7g/L, copper sulfate 0.9g/L, potassiumiodide 1.1g/L, cobalt chloride 0.25g/L, boric acid 6.8g/L, Sodium orthomolybdate 0.47g/L, vitamin V B1 0.7g/L, vitamin V B6 0.3g/L, vitamin V B5 0.4g/L, glycine 0.7g/L, ferrous sulfate 0.6 g/L, disodium ethylene diamine tetraacetate 1.4g/L, calcium chloride 1.5g/L, naphthylacetic acid 0.2g/L, banana puree 12g/L, mashed potato 20g/L, white sugar 8g/L, inositol 48g/L, agar powder 1.2g/L, 6-benzylaminopurine 0.1g/L, brassinolide 0.2g/L, paclobutrazol 0.35g/L and the sweet phosphine 0.1g/L of increasing, then be settled to 1L with distilled water.
Formula five
A kind of Herba Dendrobii nutrient solution, it contains following component: saltpetre 50g/L, ammonium nitrate 44g/L, magnesium sulfate 13g/L, manganous sulfate 5g/L, zinc sulfate 0.8g/L, potassium primary phosphate 1.8g/L, copper sulfate 1.0g/L, potassiumiodide 1.2g/L, cobalt chloride 0.3g/L, boric acid 7g/L, Sodium orthomolybdate 0.5g/L, vitamin V B1 0.75g/L, vitamin V B6 0.35g/L, vitamin V B5 0.5g/L, glycine 0.75g/L, ferrous sulfate 0.65g/L, disodium ethylene diamine tetraacetate 1.45g/L, calcium chloride 1.8g/L, naphthylacetic acid 0.25g/L, banana puree 14g/L, mashed potato 22g/L, white sugar 10g/L, inositol 50g/L, agar powder 1.4g/L, 6-benzylaminopurine 0.2g/L, brassinolide 0.3g/L, paclobutrazol 0.4g/L and the sweet phosphine 0.3g/L of increasing, then be settled to 1L with distilled water.
Experimental example 1
Under aseptic condition, get the Herba Dendrobii stem section that has axillalry bud of the long length 2～2.5cm that has 3～5 stem segments as explant, the nutrient solution and the common MS nutrient solution (formula six) that explant are placed in respectively to formula one, two, three, four, five are cultivated, culture condition: 25 DEG C of temperature, illumination condition 2000LX, light application time 12h/d, cultivate and compare after 25 days.
Table 1 polysaccharide content and upgrowth situation comparison sheet
Experimental result shows, utilizes nutrient solution of the present invention to cultivate Herba Dendrobii stem section, can significantly improve Dendrobium officinale polysaccharide content.
Experimental example 2
Under aseptic condition, get the Herba Dendrobii stem section that has axillalry bud of the long length 2～2.5cm that has 3～5 stem segments as explant, the nutrient solution and the common MS nutrient solution (formula six) that explant are placed in respectively to formula one, two, three, four, five carry out the amplification of stem section propagation, culture condition: 25 DEG C of temperature, illumination condition 2000LX, light application time 12h/d.
Table 2 stem section propagation amplification comparison sheet
Experimental result shows, utilizes nutrient solution of the present invention to cultivate Herba Dendrobii stem section, can significantly shorten culture cycle.
Claims (5)
1. a Herba Dendrobii nutrient solution, is characterized in that containing following component:
Saltpetre 47～57g/L, ammonium nitrate 40～50g/L, magnesium sulfate 10.4～20.4g/L, manganous sulfate 3.2～7.2 g/L, zinc sulfate 0.5～1.5g/L, potassium primary phosphate 1.5～2.5g/L, copper sulfate 0.7～1.7g/L, potassiumiodide 0.9～1.9 g/L, cobalt chloride 0.15～0.55g/L, boric acid 5.8～9.8g/L, Sodium orthomolybdate 0.37～0.77g/L, vitamin V B1 0.6～1.0g/L, vitamin V B6 0.25～0.65g/L, vitamin V B5 0.33～0.83g/L, glycine 0.61～1.01g/L, ferrous sulfate 0.58～0.98 g/L, disodium ethylene diamine tetraacetate 1.3～1.7g/L, calcium chloride 1.3～3.3g/L, naphthylacetic acid 0.13～0.53g/L, banana puree 10～20g/L, mashed potato 19～29g/L, white sugar 6～16g/L, inositol 46～56g/L, agar powder 1.0～2.0g/L, 6-benzylaminopurine 0.1～0.6g/L, brassinolide 0.2～0.8g/L, paclobutrazol 0.35～0.65g/L and the sweet phosphine 0.1～0.6g/L of increasing.
2. a kind of Herba Dendrobii nutrient solution as claimed in claim 1, is characterized in that containing following component:
Saltpetre 49～55g/L, ammonium nitrate 42～48g/L, magnesium sulfate 12.4～18.4g/L, manganous sulfate 4.2～6.2 g/L, zinc sulfate 0.7～1.3g/L, potassium primary phosphate 1.7～2.3g/L, copper sulfate 0.9～1.5g/L, potassiumiodide 1.1～1.7 g/L, cobalt chloride 0.25～0.45g/L, boric acid 6.8～8.8g/L, Sodium orthomolybdate 0.47～0.67g/L, vitamin V B1 0.7～0.9g/L, vitamin V B6 0.3～0.6g/L, vitamin V B5 0.4～0.8g/L, glycine 0.7～1.0g/L, ferrous sulfate 0.6～0.9 g/L, disodium ethylene diamine tetraacetate 1.4～1.6g/L, calcium chloride 1.5～3.0g/L, naphthylacetic acid 0.2～0.5g/L, banana puree 12～18g/L, mashed potato 20～28g/L, white sugar 8～14g/L, inositol 48～54g/L, agar powder 1.2～1.8g/L.
3. a kind of Herba Dendrobii nutrient solution as claimed in claim 1, is characterized in that containing following component:
Saltpetre 50～54g/L, ammonium nitrate 44～46g/L, magnesium sulfate 13～17g/L, manganous sulfate 5～6g/L, zinc sulfate 0.8～1.2g/L, potassium primary phosphate 1.8～2.2g/L, copper sulfate 1.0～1.4g/L, potassiumiodide 1.2～1.5g/L, cobalt chloride 0.3～0.4g/L, boric acid 7～8g/L, Sodium orthomolybdate 0.5～0.6g/L, vitamin V B1 0.75～0.85g/L, vitamin V B6 0.35～0.55g/L, vitamin V B5 0.5～0.7g/L, glycine 0.75～0.95g/L, ferrous sulfate 0.65～0.85g/L, disodium ethylene diamine tetraacetate 1.45～1.55g/L, calcium chloride 1.8～2.5g/L, naphthylacetic acid 0.25～0.45g/L, banana puree 14～16g/L, mashed potato 22～26g/L, white sugar 10～12g/L, inositol 50～52g/L, agar powder 1.4～1.6g/L.
4. a kind of Herba Dendrobii nutrient solution as claimed in claim 1, is characterized in that containing following component:
Saltpetre 51～53g/L, ammonium nitrate 44.5～45.5g/L, magnesium sulfate 14～16g/L, manganous sulfate 5.2～5.5g/L, zinc sulfate 1.0～1.2g/L, potassium primary phosphate 1.9～2.1g/L, copper sulfate 1.1～1.3g/L, potassiumiodide 1.3～1.4g/L, cobalt chloride 0.32～0.38g/L, boric acid 7.2～7.8g/L, Sodium orthomolybdate 0.52～0.58g/L, vitamin V B1 0.78～0.82g/L, vitamin V B6 0.45～0.52g/L, vitamin V B5 0.55～0.65g/L, glycine 0.8～0.86g/L, ferrous sulfate 0.7～0.8g/L, disodium ethylene diamine tetraacetate 1.48～1.52g/L, calcium chloride 2.0～2.2g/L, naphthylacetic acid 0.3～0.4g/L, banana puree 14.5～15.5g/L, mashed potato 24～25g/L, white sugar 11～12g/L, inositol 51～52g/L, agar powder 1.45～1.55g/L.
5. a kind of Herba Dendrobii nutrient solution as claimed in claim 1, is characterized in that containing 6-benzylaminopurine 0.2～0.4g/L, brassinolide 0.3～0.6g/L, paclobutrazol 0.4～0.5g/L and increases sweet phosphine 0.3～0.5g/L.
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Denomination of invention: A culture medium for Dendrobium officinale Granted publication date: 20140604 Pledgee: Nanxun Zhejiang rural commercial bank Limited by Share Ltd. Fuyang branch Pledgor: Hangzhou Fuyang Malaysia Tools Co.,Ltd. Registration number: Y2024980007159 |