CN104285810B - Spring sword special culture media - Google Patents

Spring sword special culture media Download PDF

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Publication number
CN104285810B
CN104285810B CN201410574154.1A CN201410574154A CN104285810B CN 104285810 B CN104285810 B CN 104285810B CN 201410574154 A CN201410574154 A CN 201410574154A CN 104285810 B CN104285810 B CN 104285810B
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parts
acid
culture media
special culture
spring sword
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CN201410574154.1A
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CN104285810A (en
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陈财宝
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Liuzhou Tianzi Horticulture Co Ltd
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Liuzhou Tianzi Horticulture Co Ltd
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  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)

Abstract

The invention discloses spring sword special culture media, prepared by the following material according to parts by weight: White substratum 70-80 part, ferric sulfate 15-20 part, EDETATE SODIUM 8-10 part, sucrose 6-8 part, glucose 6-8 part, lactoalbumin hydrolysate 8-10 part, corn embryosperm 8-12 part, Tomato juice 6-12 part, indolylacetic acid 3-5 part, indolebutyric acid 3-6 part, naphthylacetic acid 2-5 part, Chlorlacetic Acid 3-6 part, ABT root-inducing powder 1-5 part, zeatin 1-4 part, kinetin 1-4 part, isopentene aminopurine 1-3 part, Plant hormones regulators,gibberellins 1-3 part, agar 20-30 part, gac 5-15 part, water 40-50 part.Beneficial effect of the present invention is: spring sword special culture media provided by the invention, nutritious, for spring sword plant characteristic preparation, can effectively improve its surviving rate, good market prospects.

Description

Spring sword special culture media
Technical field
The present invention relates to spring sword special culture media.
Background technology
Spring sword is a mutation of Chunlan.Spring sword is close with Chunlan habit, can resist cold, can survive the winter smoothly at normal temperatures.Be distributed in Sichuan, Guizhou and Yunnan more.The ground of many stone on hillside of being born in that The undergrowth is dense, height above sea level 1000 ~ 2500 meters.It is the orchid kind that of West China of China is unique.But spring sword requires higher to growing environment, cultivate and survive difficulty, be difficult to meeting the market requirement.
Summary of the invention
Object of the present invention is exactly for above-mentioned defect of the prior art, provides spring sword special culture media.
To achieve these goals, technical scheme provided by the invention is: spring sword special culture media, prepared by the following material according to parts by weight: White substratum 70-80 part, ferric sulfate 15-20 part, EDETATE SODIUM 8-10 part, sucrose 6-8 part, glucose 6-8 part, lactoalbumin hydrolysate 8-10 part, corn embryosperm 8-12 part, Tomato juice 6-12 part, indolylacetic acid 3-5 part, indolebutyric acid 3-6 part, naphthylacetic acid 2-5 part, Chlorlacetic Acid 3-6 part, ABT root-inducing powder 1-5 part, zeatin 1-4 part, kinetin 1-4 part, isopentene aminopurine 1-3 part, Plant hormones regulators,gibberellins 1-3 part, agar 20-30 part, gac 5-15 part, water 40-50 part.
Further, above-mentioned spring sword special culture media, is prepared by the following material according to parts by weight: White substratum 75 parts, 18 parts, ferric sulfate, EDETATE SODIUM 9 parts, sucrose 7 parts, glucose 7 parts, lactoalbumin hydrolysate 9 parts, corn embryosperm 10 parts, Tomato juice 9 parts, indolylacetic acid 4 parts, indolebutyric acid 5 parts, naphthylacetic acid 4 parts, Chlorlacetic Acid 5 parts, ABT root-inducing powder 3 parts, zeatin 2 parts, kinetin 2 parts, isopentene aminopurine 2 parts, Plant hormones regulators,gibberellins 2 parts, 25 parts, agar, gac 10 parts, 45 parts, water.
Beneficial effect of the present invention is: spring sword special culture media provided by the invention, nutritious, for spring sword plant characteristic preparation, can effectively improve its surviving rate, good market prospects.
Embodiment
embodiment 1:
Spring sword special culture media, is prepared by the following material according to parts by weight: White substratum 70-80 part, ferric sulfate 15-20 part, EDETATE SODIUM 8-10 part, sucrose 6-8 part, glucose 6-8 part, lactoalbumin hydrolysate 8-10 part, corn embryosperm 8-12 part, Tomato juice 6-12 part, indolylacetic acid 3-5 part, indolebutyric acid 3-6 part, naphthylacetic acid 2-5 part, Chlorlacetic Acid 3-6 part, ABT root-inducing powder 1-5 part, zeatin 1-4 part, kinetin 1-4 part, isopentene aminopurine 1-3 part, Plant hormones regulators,gibberellins 1-3 part, agar 20-30 part, gac 5-15 part, water 40-50 part.
embodiment 2:
Spring sword special culture media, is prepared by the following material according to parts by weight: White substratum 75 parts, 18 parts, ferric sulfate, EDETATE SODIUM 9 parts, sucrose 7 parts, glucose 7 parts, lactoalbumin hydrolysate 9 parts, corn embryosperm 10 parts, Tomato juice 9 parts, indolylacetic acid 4 parts, indolebutyric acid 5 parts, naphthylacetic acid 4 parts, Chlorlacetic Acid 5 parts, ABT root-inducing powder 3 parts, zeatin 2 parts, kinetin 2 parts, isopentene aminopurine 2 parts, Plant hormones regulators,gibberellins 2 parts, 25 parts, agar, gac 10 parts, 45 parts, water.
Last it is noted that the foregoing is only the preferred embodiments of the present invention, be not limited to the present invention, although with reference to previous embodiment to invention has been detailed description, for a person skilled in the art, it still can be modified to the technical scheme described in foregoing embodiments, or carries out equivalent replacement to wherein portion of techniques feature.Within the spirit and principles in the present invention all, any amendment done, equivalent replacement, improvement etc., all should be included within protection scope of the present invention.

Claims (2)

1. spring sword special culture media, it is characterized in that, prepared by the following material according to parts by weight: White substratum 70-80 part, ferric sulfate 15-20 part, EDETATE SODIUM 8-10 part, sucrose 6-8 part, glucose 6-8 part, lactoalbumin hydrolysate 8-10 part, corn embryosperm 8-12 part, Tomato juice 6-12 part, indolylacetic acid 3-5 part, indolebutyric acid 3-6 part, naphthylacetic acid 2-5 part, Chlorlacetic Acid 3-6 part, ABT root-inducing powder 1-5 part, zeatin 1-4 part, kinetin 1-4 part, isopentene aminopurine 1-3 part, Plant hormones regulators,gibberellins 1-3 part, agar 20-30 part, gac 5-15 part, water 40-50 part.
2. spring sword special culture media according to claim 1, it is characterized in that, prepared by the following material according to parts by weight: White substratum 75 parts, 18 parts, ferric sulfate, EDETATE SODIUM 9 parts, sucrose 7 parts, glucose 7 parts, lactoalbumin hydrolysate 9 parts, corn embryosperm 10 parts, Tomato juice 9 parts, indolylacetic acid 4 parts, indolebutyric acid 5 parts, naphthylacetic acid 4 parts, Chlorlacetic Acid 5 parts, ABT root-inducing powder 3 parts, zeatin 2 parts, kinetin 2 parts, isopentene aminopurine 2 parts, Plant hormones regulators,gibberellins 2 parts, 25 parts, agar, gac 10 parts, 45 parts, water.
CN201410574154.1A 2014-10-24 2014-10-24 Spring sword special culture media Active CN104285810B (en)

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CN104285810B true CN104285810B (en) 2016-01-20

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Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1404724A (en) * 2002-11-07 2003-03-26 中国科学院武汉植物研究所 Cymbidium quick-acting breeding method
CN102976848A (en) * 2012-11-30 2013-03-20 富阳市文曲生态农业开发有限公司 Dendrobium officinale culture solution
RU2012145631A (en) * 2012-10-25 2014-04-27 Федеральное государственное бюджетное образовательное учреждение высшего профессионального образования "Мордовский государственный университет им. Н.П. Огарёва" METHOD FOR REPRODUCING CYBBIDIUM IN VITRO
CN103755461A (en) * 2013-12-23 2014-04-30 佛山市顺德区今日景艺生物科技有限公司 Culture medium for culturing orchid tissue and culture method thereof

Family Cites Families (1)

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JPH05336955A (en) * 1992-06-11 1993-12-21 Nakano Vinegar Co Ltd Medium for tissue culture of plant and method for growth using the same medium

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1404724A (en) * 2002-11-07 2003-03-26 中国科学院武汉植物研究所 Cymbidium quick-acting breeding method
RU2012145631A (en) * 2012-10-25 2014-04-27 Федеральное государственное бюджетное образовательное учреждение высшего профессионального образования "Мордовский государственный университет им. Н.П. Огарёва" METHOD FOR REPRODUCING CYBBIDIUM IN VITRO
CN102976848A (en) * 2012-11-30 2013-03-20 富阳市文曲生态农业开发有限公司 Dendrobium officinale culture solution
CN103755461A (en) * 2013-12-23 2014-04-30 佛山市顺德区今日景艺生物科技有限公司 Culture medium for culturing orchid tissue and culture method thereof

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
Orchids: Advances in Tissue Culture, Genetics,Phytochemistry and Transgenic Biotechnology;Jaime A.等;《Floriculture and Ornamental Biotechnology》;20131231;第7卷(第1期);第1-14页 *
国兰无菌快繁体系的研究进展;殷静等;《安徽农业科学》;20101231;第38卷(第32期);第18071 -18073页 *
国兰组织培养研究进展;于永畅等;《山东林业科技》;20121231(第4期);第100-103页 *
春兰、豆瓣兰、莲瓣兰和春剑4种国兰种子萌发特性研究初报;庞惠仙等;《西部林业科学》;20100930;第39卷(第3期);第56-60页 *

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