CN104304020B - Cymbidium qiubeiense special culture media - Google Patents
Cymbidium qiubeiense special culture media Download PDFInfo
- Publication number
- CN104304020B CN104304020B CN201410573635.0A CN201410573635A CN104304020B CN 104304020 B CN104304020 B CN 104304020B CN 201410573635 A CN201410573635 A CN 201410573635A CN 104304020 B CN104304020 B CN 104304020B
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- CN
- China
- Prior art keywords
- parts
- qiubeiense
- cymbidium
- acid
- culture media
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- 241000596781 Cymbidium qiubeiense Species 0.000 title claims abstract description 16
- 239000001963 growth medium Substances 0.000 title claims abstract description 13
- PRPINYUDVPFIRX-UHFFFAOYSA-N 1-naphthaleneacetic acid Chemical compound C1=CC=C2C(CC(=O)O)=CC=CC2=C1 PRPINYUDVPFIRX-UHFFFAOYSA-N 0.000 claims abstract description 7
- 229920001817 Agar Polymers 0.000 claims abstract description 7
- 229930191978 Gibberellin Natural products 0.000 claims abstract description 7
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims abstract description 7
- FAIXYKHYOGVFKA-UHFFFAOYSA-N Kinetin Natural products N=1C=NC=2N=CNC=2C=1N(C)C1=CC=CO1 FAIXYKHYOGVFKA-UHFFFAOYSA-N 0.000 claims abstract description 7
- CFHLRGJWBNKBNG-UHFFFAOYSA-N NC1=NC=C2NC=NC2=N1.C=CC(C)C Chemical compound NC1=NC=C2NC=NC2=N1.C=CC(C)C CFHLRGJWBNKBNG-UHFFFAOYSA-N 0.000 claims abstract description 7
- 229930006000 Sucrose Natural products 0.000 claims abstract description 7
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 claims abstract description 7
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 claims abstract description 7
- 235000002017 Zea mays subsp mays Nutrition 0.000 claims abstract description 7
- 239000002253 acid Substances 0.000 claims abstract description 7
- 239000008272 agar Substances 0.000 claims abstract description 7
- 235000005822 corn Nutrition 0.000 claims abstract description 7
- 239000003448 gibberellin Substances 0.000 claims abstract description 7
- IXORZMNAPKEEDV-OBDJNFEBSA-N gibberellin A3 Chemical class C([C@@]1(O)C(=C)C[C@@]2(C1)[C@H]1C(O)=O)C[C@H]2[C@]2(C=C[C@@H]3O)[C@H]1[C@]3(C)C(=O)O2 IXORZMNAPKEEDV-OBDJNFEBSA-N 0.000 claims abstract description 7
- 239000008103 glucose Substances 0.000 claims abstract description 7
- 239000000413 hydrolysate Substances 0.000 claims abstract description 7
- SEOVTRFCIGRIMH-UHFFFAOYSA-N indole-3-acetic acid Chemical compound C1=CC=C2C(CC(=O)O)=CNC2=C1 SEOVTRFCIGRIMH-UHFFFAOYSA-N 0.000 claims abstract description 7
- 230000001939 inductive effect Effects 0.000 claims abstract description 7
- RUTXIHLAWFEWGM-UHFFFAOYSA-H iron(3+) sulfate Chemical compound [Fe+3].[Fe+3].[O-]S([O-])(=O)=O.[O-]S([O-])(=O)=O.[O-]S([O-])(=O)=O RUTXIHLAWFEWGM-UHFFFAOYSA-H 0.000 claims abstract description 7
- 229910000360 iron(III) sulfate Inorganic materials 0.000 claims abstract description 7
- QANMHLXAZMSUEX-UHFFFAOYSA-N kinetin Chemical compound N=1C=NC=2N=CNC=2C=1NCC1=CC=CO1 QANMHLXAZMSUEX-UHFFFAOYSA-N 0.000 claims abstract description 7
- 229960001669 kinetin Drugs 0.000 claims abstract description 7
- 239000000463 material Substances 0.000 claims abstract description 7
- 239000003375 plant hormone Substances 0.000 claims abstract description 7
- 239000000843 powder Substances 0.000 claims abstract description 7
- 239000005720 sucrose Substances 0.000 claims abstract description 7
- UEUXEKPTXMALOB-UHFFFAOYSA-J tetrasodium;2-[2-[bis(carboxylatomethyl)amino]ethyl-(carboxylatomethyl)amino]acetate Chemical compound [Na+].[Na+].[Na+].[Na+].[O-]C(=O)CN(CC([O-])=O)CCN(CC([O-])=O)CC([O-])=O UEUXEKPTXMALOB-UHFFFAOYSA-J 0.000 claims abstract description 7
- 235000015193 tomato juice Nutrition 0.000 claims abstract description 7
- UZKQTCBAMSWPJD-UQCOIBPSSA-N trans-Zeatin Natural products OCC(/C)=C\CNC1=NC=NC2=C1N=CN2 UZKQTCBAMSWPJD-UQCOIBPSSA-N 0.000 claims abstract description 7
- UZKQTCBAMSWPJD-FARCUNLSSA-N trans-zeatin Chemical compound OCC(/C)=C/CNC1=NC=NC2=C1N=CN2 UZKQTCBAMSWPJD-FARCUNLSSA-N 0.000 claims abstract description 7
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 7
- 229940023877 zeatin Drugs 0.000 claims abstract description 7
- 241000209149 Zea Species 0.000 claims 2
- 240000008042 Zea mays Species 0.000 abstract description 5
- 230000009286 beneficial effect Effects 0.000 abstract description 2
- 235000008935 nutritious Nutrition 0.000 abstract description 2
- 102000002322 Egg Proteins Human genes 0.000 description 1
- 108010000912 Egg Proteins Proteins 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 240000007594 Oryza sativa Species 0.000 description 1
- 235000007164 Oryza sativa Nutrition 0.000 description 1
- VQKFNUFAXTZWDK-UHFFFAOYSA-N alpha-methylfuran Natural products CC1=CC=CO1 VQKFNUFAXTZWDK-UHFFFAOYSA-N 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 239000003205 fragrance Substances 0.000 description 1
- 238000000034 method Methods 0.000 description 1
- 210000004681 ovum Anatomy 0.000 description 1
- 235000009566 rice Nutrition 0.000 description 1
Abstract
The invention discloses Cymbidium qiubeiense special culture media, prepared by the following material according to parts by weight: White substratum 70-80 part, ferric sulfate 15-20 part, EDETATE SODIUM 8-10 part, sucrose 6-8 part, glucose 6-8 part, lactoalbumin hydrolysate 8-10 part, corn embryosperm 8-12 part, Tomato juice 6-12 part, Sucus Cocois 3-12 part, indolylacetic acid 3-5 part, naphthylacetic acid 2-5 part, Chlorlacetic Acid 3-6 part, ABT root-inducing powder 1-5 part, zeatin 1-4 part, kinetin 1-4 part, isopentene aminopurine 1-3 part, Plant hormones regulators,gibberellins 1-3 part, agar 20-30 part, gac 5-15 part, water 40-50 part.Beneficial effect of the present invention is: Cymbidium qiubeiense special culture media provided by the invention, nutritious, for the preparation of Cymbidium qiubeiense plant characteristic, can effectively improve its surviving rate, good market prospects.
Description
Technical field
The present invention relates to Cymbidium qiubeiense special culture media.
Background technology
Cymbidium qiubeiense, the raw plant in ground; Pseudobulb is less, contains within the sheath of livid purple brown; Sheath is avette to ovum shape lanceolar, leaf 2-3 piece, band shape, and dark green and be with dirty purple, scape sends in pseudobulb base portion sheath, and uprightly, long 25-30 centimetres, purple, dredge raw 56 flowers, flower has fragrance, the florescence 10-12 month.Produce southwestern Guizho and southeastern Yunnan.Be born in sylvan life, height above sea level 700-1800 rice.It requires higher to growing environment, cultivates and survives difficulty, be difficult to meeting the market requirement.
Summary of the invention
Object of the present invention is exactly for above-mentioned defect of the prior art, provides Cymbidium qiubeiense special culture media.
To achieve these goals, technical scheme provided by the invention is: Cymbidium qiubeiense special culture media, prepared by the following material according to parts by weight: White substratum 70-80 part, ferric sulfate 15-20 part, EDETATE SODIUM 8-10 part, sucrose 6-8 part, glucose 6-8 part, lactoalbumin hydrolysate 8-10 part, corn embryosperm 8-12 part, Tomato juice 6-12 part, Sucus Cocois 3-12 part, indolylacetic acid 3-5 part, naphthylacetic acid 2-5 part, Chlorlacetic Acid 3-6 part, ABT root-inducing powder 1-5 part, zeatin 1-4 part, kinetin 1-4 part, isopentene aminopurine 1-3 part, Plant hormones regulators,gibberellins 1-3 part, agar 20-30 part, gac 5-15 part, water 40-50 part.
Further, above-mentioned Cymbidium qiubeiense special culture media, is prepared by the following material according to parts by weight: White substratum 75 parts, 18 parts, ferric sulfate, EDETATE SODIUM 9 parts, sucrose 7 parts, glucose 7 parts, lactoalbumin hydrolysate 9 parts, corn embryosperm 10 parts, Tomato juice 9 parts, Sucus Cocois 8 parts, indolylacetic acid 4 parts, naphthylacetic acid 4 parts, Chlorlacetic Acid 5 parts, ABT root-inducing powder 3 parts, zeatin 2 parts, kinetin 2 parts, isopentene aminopurine 2 parts, Plant hormones regulators,gibberellins 2 parts, 25 parts, agar, gac 10 parts, 45 parts, water.
Beneficial effect of the present invention is: Cymbidium qiubeiense special culture media provided by the invention, nutritious, for the preparation of Cymbidium qiubeiense plant characteristic, can effectively improve its surviving rate, good market prospects.
Embodiment
embodiment 1:
Cymbidium qiubeiense special culture media, prepared by the following material according to parts by weight: White substratum 70-80 part, ferric sulfate 15-20 part, EDETATE SODIUM 8-10 part, sucrose 6-8 part, glucose 6-8 part, lactoalbumin hydrolysate 8-10 part, corn embryosperm 8-12 part, Tomato juice 6-12 part, Sucus Cocois 3-12 part, indolylacetic acid 3-5 part, naphthylacetic acid 2-5 part, Chlorlacetic Acid 3-6 part, ABT root-inducing powder 1-5 part, zeatin 1-4 part, kinetin 1-4 part, isopentene aminopurine 1-3 part, Plant hormones regulators,gibberellins 1-3 part, agar 20-30 part, gac 5-15 part, water 40-50 part.
embodiment 2:
Cymbidium qiubeiense special culture media, is prepared by the following material according to parts by weight: White substratum 75 parts, 18 parts, ferric sulfate, EDETATE SODIUM 9 parts, sucrose 7 parts, glucose 7 parts, lactoalbumin hydrolysate 9 parts, corn embryosperm 10 parts, Tomato juice 9 parts, Sucus Cocois 8 parts, indolylacetic acid 4 parts, naphthylacetic acid 4 parts, Chlorlacetic Acid 5 parts, ABT root-inducing powder 3 parts, zeatin 2 parts, kinetin 2 parts, isopentene aminopurine 2 parts, Plant hormones regulators,gibberellins 2 parts, 25 parts, agar, gac 10 parts, 45 parts, water.
Last it is noted that the foregoing is only the preferred embodiments of the present invention, be not limited to the present invention, although with reference to previous embodiment to invention has been detailed description, for a person skilled in the art, it still can be modified to the technical scheme described in foregoing embodiments, or carries out equivalent replacement to wherein portion of techniques feature.Within the spirit and principles in the present invention all, any amendment done, equivalent replacement, improvement etc., all should be included within protection scope of the present invention.
Claims (2)
1. Cymbidium qiubeiense special culture media, it is characterized in that, prepared by the following material according to parts by weight: White substratum 70-80 part, ferric sulfate 15-20 part, EDETATE SODIUM 8-10 part, sucrose 6-8 part, glucose 6-8 part, lactoalbumin hydrolysate 8-10 part, corn embryosperm 8-12 part, Tomato juice 6-12 part, Sucus Cocois 3-12 part, indolylacetic acid 3-5 part, naphthylacetic acid 2-5 part, Chlorlacetic Acid 3-6 part, ABT root-inducing powder 1-5 part, zeatin 1-4 part, kinetin 1-4 part, isopentene aminopurine 1-3 part, Plant hormones regulators,gibberellins 1-3 part, agar 20-30 part, gac 5-15 part, water 40-50 part.
2. Cymbidium qiubeiense special culture media according to claim 1, it is characterized in that, prepared by the following material according to parts by weight: White substratum 75 parts, 18 parts, ferric sulfate, EDETATE SODIUM 9 parts, sucrose 7 parts, glucose 7 parts, lactoalbumin hydrolysate 9 parts, corn embryosperm 10 parts, Tomato juice 9 parts, Sucus Cocois 8 parts, indolylacetic acid 4 parts, naphthylacetic acid 4 parts, Chlorlacetic Acid 5 parts, ABT root-inducing powder 3 parts, zeatin 2 parts, kinetin 2 parts, isopentene aminopurine 2 parts, Plant hormones regulators,gibberellins 2 parts, 25 parts, agar, gac 10 parts, 45 parts, water.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410573635.0A CN104304020B (en) | 2014-10-24 | 2014-10-24 | Cymbidium qiubeiense special culture media |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410573635.0A CN104304020B (en) | 2014-10-24 | 2014-10-24 | Cymbidium qiubeiense special culture media |
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| Publication Number | Publication Date |
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| CN104304020A CN104304020A (en) | 2015-01-28 |
| CN104304020B true CN104304020B (en) | 2016-01-20 |
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| CN201410573635.0A Expired - Fee Related CN104304020B (en) | 2014-10-24 | 2014-10-24 | Cymbidium qiubeiense special culture media |
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Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN105359970A (en) * | 2015-10-15 | 2016-03-02 | 南宁旺弘生物科技有限公司 | Cymbidium orchid culture medium and preparation method thereof |
| CN106106008A (en) * | 2016-08-30 | 2016-11-16 | 四川聚峰谷农业科技开发有限公司 | A kind of tree Fructus Vitis viniferae fast seedling-cultivating method |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| RU2012145631A (en) * | 2012-10-25 | 2014-04-27 | Федеральное государственное бюджетное образовательное учреждение высшего профессионального образования "Мордовский государственный университет им. Н.П. Огарёва" | METHOD FOR REPRODUCING CYBBIDIUM IN VITRO |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH05336955A (en) * | 1992-06-11 | 1993-12-21 | Nakano Vinegar Co Ltd | Medium for tissue culture of plant and method for growth using the same medium |
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Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| RU2012145631A (en) * | 2012-10-25 | 2014-04-27 | Федеральное государственное бюджетное образовательное учреждение высшего профессионального образования "Мордовский государственный университет им. Н.П. Огарёва" | METHOD FOR REPRODUCING CYBBIDIUM IN VITRO |
Non-Patent Citations (2)
| Title |
|---|
| 国兰无菌快繁体系的研究进展;殷静等;《安徽农业科学》;20101231;第38卷(第32期);第18071-18073页 * |
| 国兰组织培养研究进展;于永畅等;《山东林业科技》;20121231(第4期);第100-103页 * |
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| CN104304020A (en) | 2015-01-28 |
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Effective date of registration: 20171018 Address after: Calculation of business R & D center eleven layer research room 530029 Nanning city the Guangxi Zhuang Autonomous Region Qingxiu District Bauhinia Road No. 1 Nanning international internet dedicated channel and cloud Patentee after: GUANGXI YIMU AGRICULTURAL TECHNOLOGY Co.,Ltd. Address before: 545002, the Guangxi Zhuang Autonomous Region, Liuzhou North Long Pond flower demonstration Office Patentee before: LIUZHOU TIANZI HORTICULTURE Co.,Ltd. |
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| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20160120 |