CN102959078B - 内源启动子在表达异源蛋白中的用途 - Google Patents

内源启动子在表达异源蛋白中的用途 Download PDF

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Publication number
CN102959078B
CN102959078B CN201180029129.XA CN201180029129A CN102959078B CN 102959078 B CN102959078 B CN 102959078B CN 201180029129 A CN201180029129 A CN 201180029129A CN 102959078 B CN102959078 B CN 102959078B
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sequence
cells
heterologous protein
protein
endogenous
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CN102959078A (zh
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G.戴维斯
D.马尔科夫
N.津泽
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Sigma Aldrich Co LLC
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Sigma Aldrich Co LLC
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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
    • C07K14/4701—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals not used
    • C07K14/4702—Regulators; Modulating activity
    • C07K14/4705—Regulators; Modulating activity stimulating, promoting or activating activity
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
    • C12N15/907—Stable introduction of foreign DNA into chromosome using homologous recombination in mammalian cells
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/502—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing non-proliferative effects
    • G01N33/5035—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing non-proliferative effects on sub-cellular localization
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/502—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing non-proliferative effects
    • G01N33/5041—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing non-proliferative effects involving analysis of members of signalling pathways
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/582—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with fluorescent label
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/60—Fusion polypeptide containing spectroscopic/fluorescent detection, e.g. green fluorescent protein [GFP]
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction
    • C07K2319/72—Fusion polypeptide containing domain for protein-protein interaction containing SH2 domain
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2799/00—Uses of viruses
    • C12N2799/02—Uses of viruses as vector
    • C12N2799/021—Uses of viruses as vector for the expression of a heterologous nucleic acid
    • C12N2799/027—Uses of viruses as vector for the expression of a heterologous nucleic acid where the vector is derived from a retrovirus

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Immunology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • General Engineering & Computer Science (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Toxicology (AREA)
  • General Physics & Mathematics (AREA)
  • Food Science & Technology (AREA)
  • Pathology (AREA)
  • Analytical Chemistry (AREA)
  • Plant Pathology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Mycology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Peptides Or Proteins (AREA)
CN201180029129.XA 2010-04-13 2011-04-13 内源启动子在表达异源蛋白中的用途 Active CN102959078B (zh)

Applications Claiming Priority (15)

Application Number Priority Date Filing Date Title
US32370210P 2010-04-13 2010-04-13
US32369810P 2010-04-13 2010-04-13
US32371910P 2010-04-13 2010-04-13
US61/323698 2010-04-13
US61/323702 2010-04-13
US61/323719 2010-04-13
US36701710P 2010-07-23 2010-07-23
US61/367017 2010-07-23
US39066810P 2010-10-07 2010-10-07
US61/390668 2010-10-07
US40885610P 2010-11-01 2010-11-01
US61/408856 2010-11-01
US201161431957P 2011-01-12 2011-01-12
US61/431957 2011-01-12
PCT/US2011/032216 WO2011130345A1 (en) 2010-04-13 2011-04-13 Use of endogenous promoters to express heterologous proteins

Publications (2)

Publication Number Publication Date
CN102959078A CN102959078A (zh) 2013-03-06
CN102959078B true CN102959078B (zh) 2016-01-20

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CN201711044283.XA Pending CN107805278A (zh) 2010-04-13 2011-04-13 产生内源标记的蛋白的方法
CN2011800291603A Pending CN102971421A (zh) 2010-04-13 2011-04-13 产生内源标记的蛋白的方法
CN201180029129.XA Active CN102959078B (zh) 2010-04-13 2011-04-13 内源启动子在表达异源蛋白中的用途

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CN201711044283.XA Pending CN107805278A (zh) 2010-04-13 2011-04-13 产生内源标记的蛋白的方法
CN2011800291603A Pending CN102971421A (zh) 2010-04-13 2011-04-13 产生内源标记的蛋白的方法

Country Status (10)

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US (5) US20130059362A1 (enExample)
EP (2) EP2558574B1 (enExample)
JP (2) JP5841996B2 (enExample)
KR (2) KR20130054955A (enExample)
CN (3) CN107805278A (enExample)
BR (1) BR112012026379A2 (enExample)
CA (2) CA2795636C (enExample)
DK (1) DK2558575T3 (enExample)
ES (1) ES2565216T3 (enExample)
WO (3) WO2011130343A1 (enExample)

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CN106800602B (zh) * 2017-01-22 2020-05-01 中国科学院生物物理研究所 一种可视化标记基因组位点的方法
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CN112105736A (zh) * 2018-05-04 2020-12-18 西格马-奥尔德里奇有限责任公司 生产具有降低宿主细胞蛋白水平的重组蛋白
JP7233545B2 (ja) * 2018-12-30 2023-03-06 エフ． ホフマン－ラ ロシュ アーゲー 標的タンパク質への検出可能なタグのＣＲＩＳＰＲ／Ｃａｓ制御組み込みに基づく細胞の選択方法
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Also Published As

Publication number Publication date
JP5841997B2 (ja) 2016-01-13
CA2795636C (en) 2018-07-31
CA2795636A1 (en) 2011-10-20
EP2558575A4 (en) 2013-10-30
JP5841996B2 (ja) 2016-01-13
EP2558575B1 (en) 2015-12-16
WO2011130345A1 (en) 2011-10-20
KR20130055588A (ko) 2013-05-28
KR20130054955A (ko) 2013-05-27
US20130065310A1 (en) 2013-03-14
EP2558574A1 (en) 2013-02-20
EP2558575A1 (en) 2013-02-20
CN102959078A (zh) 2013-03-06
DK2558575T3 (en) 2016-03-21
JP2013523181A (ja) 2013-06-17
CA2795643A1 (en) 2011-10-20
EP2558574A4 (en) 2013-10-30
JP2013523180A (ja) 2013-06-17
WO2011130343A1 (en) 2011-10-20
CA2795643C (en) 2018-07-17
CN107805278A (zh) 2018-03-16
US20130059362A1 (en) 2013-03-07
CN102971421A (zh) 2013-03-13
ES2565216T3 (es) 2016-04-01
WO2011130346A1 (en) 2011-10-20
US20180134759A1 (en) 2018-05-17
BR112012026379A2 (pt) 2015-09-22
US20130059388A1 (en) 2013-03-07
EP2558574B1 (en) 2015-03-18
US20180105564A1 (en) 2018-04-19

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