CN102910968A - Compound biological fertilizer - Google Patents
Compound biological fertilizer Download PDFInfo
- Publication number
- CN102910968A CN102910968A CN2012101263486A CN201210126348A CN102910968A CN 102910968 A CN102910968 A CN 102910968A CN 2012101263486 A CN2012101263486 A CN 2012101263486A CN 201210126348 A CN201210126348 A CN 201210126348A CN 102910968 A CN102910968 A CN 102910968A
- Authority
- CN
- China
- Prior art keywords
- sporeformer
- ball
- dewaters
- fertilizer
- embedding
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Fertilizers (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention provides a compound biological fertilizer and a preparation method thereof. The compound biological fertilizer is made from a compound fertilizer and a dehydrated spore bacterium ball in mass percentage of (6.7-16.7):1. The compound biological fertilizer can prevent outside influence on beneficial bacteria.
Description
Technical field
The present invention relates to the composite bio-fertilizer technical field, particularly a kind of composite bio-fertilizer and preparation method.
Background technology
Composite bio-fertilizer is bound organically in one to inorganic nutritive element, microbial bacteria, embody the net effect of common fertilizer and microbial fertilizer, to dissolve the soil compaction phenomenon, repair and conditioning soil, improve the chemical fertilizer utilization ratio, reduce river pollution, reduce disease and pest and occur, strengthen the anti-adversity ability of farm crop, improve the effective way of farm crop fruit quality and output.Composite bio-fertilizer is applied farm crop, can improve the effective rate of utilization of chemical fertilizer, and utilization rate of fertilizer can improve 10～30%.
The functional characteristics of composite bio-fertilizer mainly contains the following aspects: the one, and nutrition is comparatively comprehensive.Can provide part nutritive element and the active bacterium of beneficial microorganism for crop; The 2nd, fertilizer efficiency has the effect of slowly-releasing, improves the utilization ratio of fertilizer; The 3rd, chessom, molten phosphorus potassium decomposing, culture fertility.After fertilizer enters soil, microorganism is amount reproduction under the assistance of inorganic nutritive element, moisture, temperature, reduced the living space of harmful microorganism colony, thereby increased the quantity of soil beneficial microorganism bacterium, microbial bacteria produces a large amount of organic acids and can be partly dissolved to discharge for crop and again absorb being deposited on for many years phosphorus potassium element in the soil, and soil will become more and more loose and fertile behind the life-time service; The 4th, the anti-continuous cropping of diseases prevention insect protected.Microbial bacteria is in dormant state in fertilizer, after entering soil sprouting breeding, secrete a large amount of materials such as chitinase, extracellular enzyme and microbiotic, the effective conidial cell wall of cracking harmful fungoid, the growth of line eggs wall and inhibition harmful bacteria, control soil passes the generation of venereal disease insect pest effectively, plays the effect of diseases prevention insect protected and anti-continuous cropping; The 5th, strong plantlets and rootage reduces nitrite content, improves quality, increasing both production and income.After probiotics is bred in soil, produce a large amount of plant hormone and organic acid, stimulate root growth and development, strengthen the rate of photosynthisis of farm crop, plant growth is well established and vigorously developing, but the pol of Effective Raise crop and fruit, the content of nitrate and other objectionable impurities in the reduction crop product, improve quality, promote increasing crop yield; The 6th, accelerate the degraded of soil with organic matter, not only for crop growth provides more organic nutrient substances, improve the resistance of farm crop, can also reduce the living space of some pathogeny bacterium in the soil simultaneously.
Although the beneficial microorganism effect is unquestionable, but because probiotics is difficult to resist the osmotic pressure stress of chemical fertilizer, in the production process of composite bio-fertilizer, very easily the height because of the external world oozes the environment inactivation, does not reach due growth promoting function in application.
Summary of the invention
The invention provides a kind of composite bio-fertilizer and preparation method, can make probiotics avoid ectocine.
To achieve these goals, the invention provides following technical scheme:
A kind of composite bio-fertilizer, it is comprised of composite fertilizer and the sporeformer ball that dewaters, and according to mass ratio, described composite fertilizer and the sporeformer ball ratio that dewaters are 6.7～16.7: 1; The wherein said gemma coccus that dewaters is: the sporeformer ball that dewaters that subtilis is made dewaters that sporeformer ball and gel-shaped series bacillus make is according to the mixing of 1: 1.1～1.3 mass ratioes sporeformer ball that dewaters;
Described composite fertilizer contains according to mass percent: nitrogen element 16%, contains potassium element 16% at phosphorus element-containing 16%;
The described mixing sporeformer ball that dewaters is made by bacterial strain gemma and embedding reagent, count by volume, bacterial strain gemma: embedding reagent=1: 8-1: 5, wherein: the bacterial strain gemma is the bacillus liquid that subtilis and gel-shaped series bacillus ferment respectively and generate, described embedding reagent comprises the first embedding reagent: according to mass percent, sodium alginate concentration is 2%～4% the aqueous solution; The second embedding reagent: according to mass percent, CaCl
2Concentration is 2%～4% the aqueous solution.
The present invention also provides a kind of preparation method of composite microbe fertilizer, and it comprises:
1) with subtilis and gel-shaped series bacillus respectively in fermentation of bacillus liquid fermentation be prepared into the first sporeformer liquid and the second sporeformer liquid; These two kinds of genus bacillus are formed the impact of gemma by investigating different carbon sources, nitrogenous source, inorganic salt, pH, temperature, incubation time, inoculum size, drawing its sporiferous optimal medium consists of: 1～3% wheat bran, 1～3% soy peptone, 0.5～2%CaCl
2, 0.5～2%NaCl, optimal culture condition on this basis are pH7.0～7.4, and temperature is 35～45 ℃, and fermentation time is 24～72h, and inoculum size is 1～4%.
2) configuration of embedding reagent by the fixing condition optimizing of embedding, draws sodium alginate concentration in the aqueous solution of the first embedding reagent and is the CaCl in the aqueous solution of the 2～4%, second embedding reagent
2Concentration is 2～4%, CaCl
2Fixedly the time of sodium alginate is 10～24h;
3) with step 1) preparation the first sporeformer liquid and the second sporeformer liquid join respectively step 2) the first embedding reagent in, fully mix, mixed solution is joined in the second embedding reagent, namely obtaining particle diameter is the moisture sporeformer ball of 1～5mm, portion rate meter by volume, bacterial strain gemma: embedding reagent=1: 8～1: 5;
The moisture sporeformer ball of prepared 1～5mm immobilization 10～24h under 4 ℃ of environment removes CaCl with sterile water wash
2, be drying to obtain the sporeformer ball that dewaters of 0.5-2mm in 40 ℃ of baking ovens;
4) the sporeformer ball that dewaters that will make mixes with composite fertilizer, the ratio 6.7～16.7: 1 of composite fertilizer and the sporeformer ball that dewaters, and the sporeformer ball ratio that dewaters that subtilis and gel-shaped series bacillus are made respectively is 1: 1.1～1.3.
By implementing above technical scheme, have following technique effect: composite bio-fertilizer provided by the invention and preparation method,
1, after the bacterial strain of this composite bio-fertilizer passes through fixedly embedding, realized the long-term storage of living bacteria count, its storage-stable phase is more than 90 days.
2. after this composite microbe fertilizer is manured into soil, because sodium alginate is immobilization embedded, delay stripping and the sprouting of bacterial strain, thereby avoid the high osmotic pressure impact at chemical fertilizer initial stage, microorganism sprouts under the assistance of inorganic nutritive element, moisture, temperature and amount reproduction afterwards, form synergy with chemical fertilizer, both strengthened the bio-feritlizer fertilizer efficiency, greatly improved again the chemical fertilizer utilization ratio, improve biological fertilizer efficiency thereby reach, improve the chemical fertilizer quality, reduce fertilizer amount, make common fertilizer possess the multiple effect of slowly-releasing, the feature such as efficient, eco-friendly.
3. the subtilis of this composite microbe fertilizer has the effect of organophosphorus in the degraded soil, organic phosphorus degrading in the soil can be become the inorganic phosphorus that utilizes for growth and development of plants, the gel-shaped series bacillus has good ability of dissolving potassium, can the potassium of soil mineral is free out, required for growth and development of plants.
Embodiment
Technical scheme for a better understanding of the present invention, the below describes embodiment provided by the invention in detail.
The embodiment of the invention provides a kind of composite bio-fertilizer, formed by composite fertilizer and the sporeformer ball that dewaters, according to mass ratio, described composite fertilizer and the sporeformer ball ratio that dewaters are 6.7～16.7: 1, and the wherein said gemma coccus that dewaters is: the sporeformer ball that dewaters that subtilis is made dewaters that sporeformer ball and gel-shaped series bacillus make is according to the mixing of 1: 1.1～1.3 mass ratioes sporeformer ball that dewaters.
Described composite fertilizer contains according to mass percent: nitrogen element 16%, contains potassium element 16% at phosphorus element-containing 16%.
The described sporeformer ball that dewaters is made by bacterial strain gemma and embedding reagent, count by volume, bacterial strain gemma: embedding reagent=1: 8-1: 5, wherein: the bacterial strain gemma is the bacillus liquid that subtilis and gel-shaped series bacillus ferment respectively and generate, described embedding reagent comprises the first embedding reagent: according to mass percent, sodium alginate concentration is 2～4% the aqueous solution; The second embedding reagent: according to mass percent, CaCl
2Concentration is 2～4% the aqueous solution.
Described fermentation of bacillus liquid by the optimization of substratum and culture condition, reaches more than 95% the product gemma rate of two kinds of bacterial strains subtilis and gel-shaped series bacillus difference fermentation culture.
The present invention also provides a kind of preparation method of composite microbe fertilizer,
Embodiment 1:
1) with subtilis and gel-shaped series bacillus respectively in fermentation of bacillus liquid fermentation be prepared into the first sporeformer liquid and the second sporeformer liquid; Its sporiferous substratum consists of: 3% wheat bran, 3% soy peptone, 1%CaCl
2, 1%NaCl, culture condition on this basis are pH7.4, and temperature is 40 ℃, and fermentation time is 48h, and inoculum size is that 2% product gemma rate reaches more than 95%.
2) configuration of embedding reagent, the sodium alginate concentration in the aqueous solution of the first embedding reagent are the CaCl in the aqueous solution of 3%, the second embedding reagent
2Concentration is 4%, CaCl
2Fixedly the time of sodium alginate is 18h;
3) with step 1) preparation the first sporeformer liquid and the second sporeformer liquid join respectively step 2) the first embedding reagent in, fully mix, mixed solution is joined in the second embedding reagent, namely obtaining particle diameter is the moisture sporeformer ball of 3mm, portion rate meter by volume, the bacterial strain gemma: embedding reagent=1: 7, namely the ratio of this bacterial strain gemma and the first embedding reagent is 1: 7, and the ratio of this bacterial strain gemma and the second embedding reagent is 1: 7;
The moisture sporeformer ball of prepared 3mm immobilization 18h under 4 ℃ of environment removes CaCl with sterile water wash
2, be drying to obtain the sporeformer ball that dewaters of 0.8mm in 40 ℃ of baking ovens;
4) the sporeformer ball that dewaters that will make mixes with composite fertilizer, the ratio of composite fertilizer and the sporeformer ball that dewaters 10: 1.The sporeformer ball ratio that dewaters that subtilis and gel-shaped series bacillus are made respectively is 1: 1.2.
Above-mentioned bacterial strains obtains by screening, wherein subtilis (Bacillus subtilis) is in China Committee for Culture Collection of Microorganisms's common micro-organisms center preservation, the preservation address is No. 3, Yard 1, BeiChen xi Road, Chaoyang District, Beijing City, deposit number is CGMCC NO.6041, preservation date: on April 23rd, 2012, gel-shaped series bacillus (Paenibacillus mucilaginosus) is in China Committee for Culture Collection of Microorganisms's common micro-organisms center preservation, the preservation address is No. 3, Yard 1, BeiChen xi Road, Chaoyang District, Beijing City, deposit number is CGMCC NO.6030, preservation date: on April 23rd, 2012.
Embodiment 2:
1) with subtilis and gel-shaped series bacillus respectively in fermentation of bacillus liquid fermentation be prepared into the first sporeformer liquid and the second sporeformer liquid; Its sporiferous substratum consists of: 2% wheat bran, 2% soy peptone, 0.5%CaCl
2, 0.5%NaCl, culture condition on this basis are pH7.2, and temperature is 35 ℃, and fermentation time is 72h, and inoculum size is 4%, produces the gemma rate and reaches more than 95%.
2) configuration of embedding reagent, the sodium alginate concentration in the aqueous solution of the first embedding reagent are the CaCl in the aqueous solution of 2%, the second embedding reagent
2Concentration is 3%, CaCl
2Fixedly the time of sodium alginate is 12h;
3) with step 1) preparation the first sporeformer liquid and the second sporeformer liquid join respectively step 2) the first embedding reagent in, fully mix, then join in the second embedding reagent, namely obtaining particle diameter is the moisture sporeformer ball of 3mm, portion rate meter by volume, the bacterial strain gemma: embedding reagent=1: 8, namely the ratio of this bacterial strain gemma and the first embedding reagent is 1: 8, and the ratio of this bacterial strain gemma and the second embedding reagent is 1: 8;
The moisture sporeformer ball of prepared 3mm immobilization 12h under 4 ℃ of environment removes CaCl with sterile water wash
2, be drying to obtain the sporeformer ball that dewaters of 0.8mm in 40 ℃ of baking ovens;
4) the sporeformer ball that dewaters that will make mixes with composite fertilizer, the ratio of composite fertilizer and the sporeformer ball that dewaters 12: 1.The sporeformer ball ratio that dewaters that subtilis and gel-shaped series bacillus are made respectively is 1: 1.1.
Embodiment 3
This embodiment is the Application Example of composite biological fertilizer preparation method for material.Take soil as experiment material, adopt potted plant method to detect the dissolution time of bacterial strain gemma in soil of sporeformer ball in the composite bio-fertilizer.
Concrete grammar:
Test adopts 20cm * 25cm plastic tub to carry out, and every basin fills native 10kg.
During the composite bio-fertilizer of placing 30 days was manured into soil, sowing amount was 15g, and the bacterial strain gemma total count that applies is 〉=2 * 10
8Individual, each processes 3 repetitions.
Bacterial strain sporeformer liquid mixed being sprayed onto on the Common compound fertilizer, placed 30 days, in being manured into soil in contrast.
Apply the soil sample of rear detection different time, the number that method detects bacterial strain gemma in the soil of sending out by plate count sees table 1 for details.
The bacterial strain gemma is by the embedding of embedding medium, so that bacterial strain obtains protection, and reaches the effect that well slowly discharges; do not use the bacterial strain of embedding medium embedding; because the impact of Common compound fertilizer high osmotic pressure so that the survival of bacterial strain is affected, causes the number of bacterial strain extremely low.
What composite biological fertilizer of the present invention adopted is to have functional microorganism strains, can be applicable to all soil types and farm crop, can improve biological fertilizer efficiency, improve the chemical fertilizer quality, reduce fertilizer amount, make common fertilizer possess the multiple effect of the features such as slowly-releasing, long-acting, environmental friendliness.
Above a kind of composite bio-fertilizer and the preparation method that the embodiment of the invention is provided is described in detail, for one of ordinary skill in the art, thought according to the embodiment of the invention, all will change in specific embodiments and applications, in sum, this description should not be construed as limitation of the present invention.
Claims (2)
1. a composite bio-fertilizer is characterized in that, is comprised of composite fertilizer and the sporeformer ball that dewaters, and according to mass ratio, described composite fertilizer and the sporeformer ball ratio that dewaters are 6.7～16.7: 1; The wherein said gemma coccus that dewaters is: the sporeformer ball that dewaters that subtilis is made dewaters that sporeformer ball and gel-shaped series bacillus make is according to the mixing of 1: 1.1～1.3 mass ratioes sporeformer ball that dewaters;
Described composite fertilizer contains according to mass percent: nitrogen element 16%, contains potassium element 16% at phosphorus element-containing 16%;
The described mixing sporeformer ball that dewaters is made by bacterial strain gemma and embedding reagent, count by volume, bacterial strain gemma: embedding reagent=1: 8-1: 5, wherein: the bacterial strain gemma is the bacillus liquid that subtilis and gel-shaped series bacillus ferment respectively and generate, described embedding reagent comprises the first embedding reagent: according to mass percent, sodium alginate concentration is 2%～4% the aqueous solution; The second embedding reagent: according to mass percent, CaCl
2Concentration is 2～4% the aqueous solution.
2. the preparation method of a composite microbe fertilizer is characterized in that, comprising:
1) with subtilis and gel-shaped series bacillus respectively in fermentation of bacillus liquid fermentation be prepared into the first sporeformer liquid and the second sporeformer liquid;
2) configuration of embedding reagent: by the fixing condition optimizing of embedding, draw sodium alginate concentration in the aqueous solution of the first embedding reagent and be the CaCl in the aqueous solution of the 2～4%, second embedding reagent
2Concentration is 2～4%, CaCl
2Fixedly the time of sodium alginate is 10～24h;
3) with step 1) preparation the first sporeformer liquid and the second sporeformer liquid join respectively step 2) the first embedding reagent in, fully mix, mixed solution is joined in the second embedding reagent, namely obtaining particle diameter is the moisture sporeformer ball of 1～5mm the first sporeformer liquid and the second sporeformer liquid, portion rate meter by volume, bacterial strain gemma: embedding reagent=1: 8～1: 5;
The moisture sporeformer ball of prepared 1～5mm immobilization 10～24h under 4 ℃ of environment removes CaCl with sterile water wash
2, be drying to obtain the sporeformer ball that dewaters of 0.5-2mm in 40 ℃ of baking ovens;
4) the sporeformer ball that dewaters that will make mixes with composite fertilizer, the ratio 6.7～16.7: 1 of composite fertilizer and the sporeformer ball that dewaters, and the sporeformer ball ratio that dewaters that subtilis and gel-shaped series bacillus are made respectively is 1: 1.1～1.3.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2012101263486A CN102910968A (en) | 2012-04-27 | 2012-04-27 | Compound biological fertilizer |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2012101263486A CN102910968A (en) | 2012-04-27 | 2012-04-27 | Compound biological fertilizer |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN102910968A true CN102910968A (en) | 2013-02-06 |
Family
ID=47609575
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2012101263486A Pending CN102910968A (en) | 2012-04-27 | 2012-04-27 | Compound biological fertilizer |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN102910968A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112939681A (en) * | 2021-02-02 | 2021-06-11 | 河南农业大学 | Gel microsphere based on anaerobic fermentation tail liquid and preparation method and application thereof |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1657508A (en) * | 2005-01-28 | 2005-08-24 | 杨昌树 | Rare earth biological multielement composite mixed fertilizer |
| CN101239846A (en) * | 2008-03-07 | 2008-08-13 | 山东省农业科学院土壤肥料研究所 | Embedded immobilization microbial fertilizer and its preparing method |
| CN101759501A (en) * | 2010-01-05 | 2010-06-30 | 爱沃(北京)农业技术有限公司 | Complex microorganisms flora grain type ascharite fertilizer and preparation method thereof |
-
2012
- 2012-04-27 CN CN2012101263486A patent/CN102910968A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1657508A (en) * | 2005-01-28 | 2005-08-24 | 杨昌树 | Rare earth biological multielement composite mixed fertilizer |
| CN101239846A (en) * | 2008-03-07 | 2008-08-13 | 山东省农业科学院土壤肥料研究所 | Embedded immobilization microbial fertilizer and its preparing method |
| CN101759501A (en) * | 2010-01-05 | 2010-06-30 | 爱沃(北京)农业技术有限公司 | Complex microorganisms flora grain type ascharite fertilizer and preparation method thereof |
Non-Patent Citations (2)
| Title |
|---|
| 谢宝恩等: "新型复合生物肥料的研制与应用", 《河南科学》 * |
| 马莉等: "固定枯草芽孢杆菌发酵生产类细菌素的研究", 《食品与发酵工业》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112939681A (en) * | 2021-02-02 | 2021-06-11 | 河南农业大学 | Gel microsphere based on anaerobic fermentation tail liquid and preparation method and application thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN104609995B (en) | Plant growth promoting bio-organic fertilizer for saline-alkali land | |
| CN103539535B (en) | Active biological matrix product specially used for culture of cucumber seedlings | |
| CN103636928B (en) | Method for preparing functional alga fertilizer through co-fermentation of multiple strains | |
| CN104447095B (en) | A kind of biological activity organic medical fertilizer and its preparation method and application | |
| CN104789226A (en) | Charcoal-based microbial soil conditioner and preparation method thereof | |
| CN109365495A (en) | The method of charcoal carrier surface activating agent coupling antimicrobial plant remedying oil-polluted soils | |
| CN105130685A (en) | Preparation method for organic fertilizer containing Nu-polyglutamic acid | |
| CN102249753A (en) | Method for producing multifunctional bioorganic fertilizer and application of multifunctional bioorganic fertilizer | |
| CN104291886A (en) | Composite organic fertilizer prepared from vegetable wastes and preparation method of composite organic fertilizer | |
| CN104774772B (en) | It is a kind of with the straw decomposition microbial inoculum of getting fat effect and its application | |
| CN107473786A (en) | A kind of method that rice straw returning to the field improves soil | |
| CN103725631A (en) | Straw-decomposing inoculant | |
| CN106318884A (en) | Straw organic decomposition agent and preparation method thereof | |
| CN107857664A (en) | A kind of organic farm soils improvement microbial enzyme composite bacterial fertilizer and preparation method thereof | |
| CN107244990A (en) | A kind of method for preparing alkaline land improving bio-organic fertilizer special material using extra large terrestrial resource | |
| CN107285925A (en) | A kind of method of utilization microorganism formulation stalk stack retting returning to the field | |
| CN1974490A (en) | Process of fermenting waste from farm and sideline product processing to produce fulvic acid bacterial manure | |
| CN110846261A (en) | Straw returning fast decay promoting microbial inoculum | |
| CN103159570A (en) | Solid microbial fertilizer and preparation method thereof | |
| CN107298633A (en) | A kind of stalk stack retting biological agent | |
| CN105001007A (en) | Method for manufacturing organic fertilizer with orange peels and municipal sludge as main raw materials and organic fertilizer | |
| CN102249754A (en) | Paenibacillus polymyxa microbial fertilizer produced through sweet potato starch waste water recovery | |
| CN102173899B (en) | Amino acid-containing liquid fertilizer and preparation method thereof | |
| CN109553478A (en) | A kind of biological organic fertilizer and preparation method thereof using the rape stalk exploitation dedicated biological and ecological methods to prevent plant disease, pests, and erosion function of rape | |
| CN103172415B (en) | Paenibacillus polymyxa microbial fertilizer produced through resource production of surplus sludge |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C12 | Rejection of a patent application after its publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20130206 |