CN102867560A - Electromagnetic radiation experimental device and applications thereof - Google Patents
Electromagnetic radiation experimental device and applications thereof Download PDFInfo
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- CN102867560A CN102867560A CN2012102332981A CN201210233298A CN102867560A CN 102867560 A CN102867560 A CN 102867560A CN 2012102332981 A CN2012102332981 A CN 2012102332981A CN 201210233298 A CN201210233298 A CN 201210233298A CN 102867560 A CN102867560 A CN 102867560A
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Abstract
The invention relates to an electromagnetic radiation experimental device, a damage effect evaluation method by utilizing the device, and applications of the method to screening of medicines preventing electromagnetic radiation. The device comprises an electromagnetic radiation simulation source and a biochemical incubator which are both powered by an external power supply, the biochemical incubator has a larger size, the electromagnetic radiation source is arranged in the middle of the biochemical incubator, a cultivation platform for placing experimental samples are arranged at the periphery of an emission end of the electromagnetic radiation source, the biochemical incubator is provided with a temperature controller, a temperature adjusting switch and a temperature display, the temperature fluctuation in the control incubator is plus or minus 0.5 DEG C, and the temperature uniformity is plus or minus 1 DEG C. The electromagnetic radiation source of the device comprises two types of output frequency, each of which comprises six gears that can realize 12 types of output power capable of continuously working for more than one month, and the normal growth conditions of creatures can be met. The main applications lie in that the evaluation on the electromagnetic radiation as well as screening of anti-electromagnetic radiation medicines can be conducted while ensuring the normal growth condition of animals.
Description
Technical field
The invention belongs to biological medicine health care correlative technology field, the damage effect evaluation method that is specifically related to a kind of electromagnetic radiation effect evaluating apparatus and utilizes this device to carry out, can utilize Caenorhabditis elegans to carry out the evaluation of electromagnetic radiation damage effect, and can be used for screening and the research and development of anti-electromagnetic-radiation new drug.
Background technology
Electromagnetic radiation has become a kind of new environomental pollution source.Electromagnetic radiation in people's living environment mostly is low dosage, continuation radiation, and its biological effect mostly is cumulative effect, and the harm of human health be can not be ignored.But the mechanism of electromagnetic radiation biological effect there is no final conclusion, and one of reason that it is important just is to lack responsive, effective biological effect evaluating apparatus and method.At present, multiple electromagnetic radiation generating means, can be in the laboratory, carrying out the biological effect evaluation of system, but often self heat production during the large and work of volume such as these device such as electromagnetic reverberation room, radar signal simulators etc., be difficult for being placed in the constant incubator commonly used, also be difficult to get rid of environment temperature to electromagnetic radiation related experiment result's impact.Although under the radiation condition of short time, high dose, can ignore to a certain extent environmental factor to the impact of experimental result, but for the radiation parameter of long-time, low dosage, experimental result is subject to the interference of external environment (temperature, humidity etc.) unavoidably, and existing electromagnetic radiation generating means is not all considered this disturbing factor.Therefore, in the urgent need to setting up the electromagnetic radiation effect evaluating apparatus that can satisfy animal used as test normal habitat demand, be particularly useful for the evaluation of electromagnetic radiation cumulative effect.
In the former studies of electromagnetic radiation biological effect, in vivo model and external model have been widely used, both complicacy and unicity cause resulting biological effect to be difficult to normalization, therefore in the urgent need to setting up and developing a kind of more responsive, effective galvanomagnetic effect evaluation system, so that sensitive, as to stablize, assess reliably electromagnetic radiation biohazard and mechanism satisfy the active demand of electromagnetic radiation protection medical science.Caenorhabditis elegans is that easily row, biological effect are assessed easily model organism in a kind of operation, compare with cell model commonly used in the research of electromagnetic radiation biological effect and higher mammal and to have some superiority, be in particular in that cell culture technology causes it to be difficult to electromagnetic pollution evaluation in the actual environment to the dependence of temperature, humidity, air and the condition of culture such as aseptic; And the complicacy of higher mammal causes utilizing the mammals such as mouse, rat, rabbit to carry out the short-term electromagnetic environment exposing and to be difficult to see clear and definite biological effect, and when carrying out long-term electromagnetic environment exposure assessment cumulative effect, the variation of environmental factor often causes being difficult to screen the mutually mutual interference of electromagnetic failure effect and environmental factor effect from experimental result.Use C. Elegans Automatic Screening to carry out this type of research and then have many-sided advantage, it is a kind of idealized model biology that can estimate the electromagnetic radiation biological effect, have and raise simple (normal pressure, normal oxygen, 20 ℃ of conditions get final product), breeding is (hermaphroditic, self-propagation) easily, phenotype is enriched advantages such as (having abundant physical signs, behavioral indicator, learning and memory evaluation index etc.), therefore is highly suitable for the biological effect evaluation of electromagnetic radiation from environment and the screening of electromagnetic protection medicine.
Summary of the invention
For the problems referred to above, the objective of the invention is to set up a kind of simple and easy to do electromagnetic radiation experimental provision that can be used for the electromagnetic radiation damage effect is carried out objective evaluation, can under the normal growth state, give electromagnetic radiation for the animal used as test that comprises C. Elegans Automatic Screening, thereby realize electromagnetic radiation damage Effect Evaluation method, and be further used for the examination of anti-electromagnetic-radiation medicine.
For achieving the above object, the present invention takes following technical scheme:
First aspect, the invention provides a kind of electromagnetic radiation experimental provision, comprise electromagnetic radiation dummy source and biochemical cultivation case two parts, all power by external power supply, it is characterized in that: described biochemical cultivation case has larger volume, the electromagnetic radiation dummy source is placed centre position within it, around this electromagnetic radiation dummy source transmitting terminal, establish for the cultivation platform of placing laboratory sample, and biochemical cultivation case is provided with temperature controller, temperature regulation switch and temperature and shows, temperature fluctuation is ± 0.5 ℃ in the control box, and temperature homogeneity is ± 1 ℃.
Described biochemical cultivation case is the constant incubator of 250L, and described electromagnetic radiation dummy source is the mobile phone radiation source, and overall dimensions are 210 * 190 * 80mm, and transmitting terminal is emitting antenna.
Described electromagnetic radiation dummy source is provided with 1750MHz and two kinds of transmission frequencies of 900MHz, and each frequency is respectively arranged with 0.1,0.2,0.2,0.6,0.8,1 six gear.
Described electromagnetic radiation dummy source comprises box body, and frequency generator is housed in the box, and the antenna of two kinds of frequencies of emission vertically is set on the box body, and the box body operation and control interface is established each self-corresponding frequency fork shift and power switch under frequency change-over switch, the two kinds of frequencies.
Described cultivation platform is the circular cylindrical cavity about high 2-5mm, and described laboratory sample is double dish or the culture plate that C. Elegans Automatic Screening is housed, and double dish or culture plate place on the circular cylindrical cavity; Cultivation platform is a plurality of, be distributed in internal antenna around.
Second aspect the invention provides and a kind ofly utilizes described electromagnetic radiation experimental provision to carry out electromagnetic radiation damage Effect Evaluation method, comprises the steps:
1) sets the temperature inside the box by temperature controller and temperature regulation switch, set electromagnetic radiation dummy source emissive power by the frequency fork shift;
2) laboratory sample is put in the double dish by grouping, double dish is put into the cultivation platform position of biochemical cultivation case, cultivate setting under the electromagnetic radiation condition;
3) cultivate to finish after, growth conditions of each group nematode is carried out image acquisition, respectively organize the grow difference of state of nematode, whether evaluation experimental group experiment condition has the electromagnetic radiation damage effect.
In the method, comprise C. Elegans Automatic Screening in the described laboratory sample.
The third aspect the invention provides and a kind ofly utilizes described electromagnetic radiation experimental provision to carry out the method for anti-electromagnetic-radiation medicine examination, may further comprise the steps:
1) medicine that will intend screening is mixed with the NGM culture plate of variable concentrations, bioorganism is grown grouping at this culture plate;
2) each group laboratory sample is placed in the described electromagnetic radiation experimental provision and implements electromagnetic radiation, observe the pattern of growing of respectively organizing bioorganism in the laboratory sample;
3) judge whether electromagnetic radiation has damage effect to bioorganism, and have which kind of damage effect;
4) respectively organize the pattern of growing of bioorganism, judge the variable concentrations medicine for the impact of bioorganism, examination goes out to have medicine and the drug concentration of anti-electromagnetic-radiation.
Wherein, described bioorganism is normal strain or mutating strain series C. Elegans Automatic Screening, described view mode be image acquisition and image ratio; Described impact is to grow promotion and/or the state of laying eggs.
Each organizes the medicine that laboratory sample comprises blank group, irradiation group, the medicine-control group of variant concentration, variant concentration-irradiation group.
The present invention is owing to take above technical scheme, and it has the following advantages: 1, apparatus of the present invention temperature-control range is extensive, can satisfy the normal growth needs of animal, the light easy row of electromagnetic radiation dummy source, and power is adjustable.2, simple and easy to do, the evaluation that is suitable for the electromagnetic radiation biological effect of evaluation method of the present invention and the impact that can get rid of the external environment factors such as environment temperature fully.3, use apparatus and method of the present invention, can and research and develop the directiveness effect for the screening of anti-electromagnetic-radiation new drug.
Description of drawings
Fig. 1 is electromagnetic radiation dummy source schematic appearance;
Fig. 2 is the synoptic diagram of constant incubator and electromagnetic radiation dummy source composite set;
Fig. 3 is that C. Elegans Automatic Screening developmental state under each minute set condition in electromagnetic radiation environment shows.
The growth curve of Fig. 4 Normal group (20 ℃) and 25 ℃ of temperature control group N2 nematodes
Fig. 5 control group and 25 ℃ of temperature conditions are on the lay eggs impact of distributed number and the sum of laying eggs of nematode
The growth curve of N2 nematode under Figure 61 750MHz and the 900MHz electromagnetic radiation condition
Fig. 7 electromagnetic irradiation on nematode lay eggs distributed number and lay eggs the sum impact
The state that grows of nematode behind the anemaran effect 48h of Fig. 8 variable concentrations
Fig. 9 variable concentrations anemaran is to the protection effect of electromagnetic radiation
The state that grows of nematode behind the LBP-X effect 48h of Figure 10 variable concentrations
Figure 11 variable concentrations LBP-X is to the protection effect of electromagnetic radiation
Embodiment
In order to guarantee that C. Elegans Automatic Screening in the electromagnetic radiation process is in normal growth conditions and is not subjected to the impact of environment temperature that electromagnetic radiation brings etc., the present invention adopts constant incubator to carry out the research of electromagnetic radiation biological effect with the mode that electromagnetic radiation source (mobile phone radiation source) combines.
For this reason, apparatus of the present invention are based on following design philosophy: 1, based on thermally-stabilised principle and the second law of thermodynamics of constant incubator, when local temperature in the casing of constant incubator rises, meeting is by the institute of the temperature in incubator balance, and the volume in the balanced speed of local temperature and mobile phone radiation source, the volume of incubator and the factor analysis such as temperature-control performance of incubator.When incubator volume (being designed to 250L) was far longer than the volume in mobile phone radiation source, near the local temperature the mobile phone radiation source can be balanced very soon.2, since in the market the employed radiation frequency range of mobile phone be 900MHz and 1800MHz etc., and the temperature that small size mobile phone radiation source causes rises easily by constant incubator institute balance, thereby designed contain two kinds of microwave frequencies (being respectively 1750MHz and 900MHz), output power is the small handsets radiation source of adjusting shift, its volume is 210 * 190 * 80mm, and the whip antenna height is that 100mm, diameter are 8mm; And each radiation frequency is divided into 6 grades, is respectively 0.1,0.2,0.4,0.6,0.8 and 1 grade, peak power output is in 1 file location, and its power is 2.23W, and minimum output power is in 0.1 file location, and its power is 0.008W.
Based on above-mentioned design philosophy, the present invention transforms accordingly to existing constant incubator, processed a kind of small handsets radiation source that can the analogue mobile phone radiation power, be placed on and carry out electromagnetic radiation in the constant incubator, thereby avoid the heat that produces in the electromagnetic radiation process to disturb electromagnetic radiation to the effect of C. Elegans Automatic Screening, more be conducive to carry out the research of long-time, low dosage electromagnetic radiation damage mechanism and anti-electromagnetic-radiation medicine.
Below in conjunction with drawings and Examples the present invention is described in detail.
Experimental provision of the present invention comprises biochemical cultivation case and electromagnetic radiation dummy source located therein.
The electromagnetic radiation dummy source is the miniaturized radiation source (also claiming the mobile phone radiation source) of analogue mobile phone radiation frequency, adopt 1750MHz and two kinds of transmission frequencies of 900MHz, be respectively arranged with 6 gears (0.1,0.2,0.4,0.6,0.8,1) under each frequency, can realize altogether the switching of 2 kinds of frequencies, 12 kinds of output powers, its outward appearance operation and control interface as shown in Figure 1.In conjunction with shown in Figure 2, dummy source comprises box body 1, box body is of a size of 210 * 190 * 80mm, frequency generator (not drawing among the figure) is housed in the box, the whip antenna 2,3 of 900MHz and two kinds of frequencies of 1750MHz is set perpendicular to cartridge upper surface respectively on box body 1, box body 1 operation and control interface arranges each self-corresponding frequency fork shift 5,6 under 4, two kinds of frequencies of frequency change-over switch, and power switch 7.Electromagnetic radiation dummy source output power and gear contrast are as shown in table 1.
Table 1: output power and the gear table of comparisons
It is the constant incubator of 250L that biochemical cultivation case is selected volume, comprise an airtight casing 8, in casing 8, bracing frame 15 is set is used for the box body 1 that card is put dummy source, to make box body 1 be in the central area of casing 8 uniform in casing with the electromagnetism wave energy that guarantees its emission as far as possible; Be laid with a plurality of C. Elegans Automatic Screening cultivation platforms 14 at box body 1 upper surface, this cultivation platform 14 is diameter 35mm, circular cylindrical cavity about high 2-5mm, be used for placing C. Elegans Automatic Screening double dish (or culture plate), a plurality of cultivation platforms 14 be distributed in casing 8 internal antennas 2 and antenna 3 around, cultivation platform 14 makes between electromagnetic radiation dummy source and double dish or the culture plate has certain interval to be beneficial to gas communication, avoids electromagnetic radiation dummy source self-temperature to change C. Elegans Automatic Screening in the double dish is exerted an influence; The power lead of electromagnetic radiation dummy source passes the Kong Eryu external power source 9 of casing 8 sides and connects.Casing 8 outside surfaces arrange power switch 10, temperature display 11, temperature controller 12 and temperature regulation switch 13.Temperature controller 12 is available from Hubei Province Hengfeng Medical Instruments Co., Ltd., Huangsih City, model is the SP-01 type, and has the adjusting temperature function, its temperature control main body is loaded in the casing 8, and in setting value, temperature controller 12 is connected with temperature regulation switch 13 the temperature inside the box by temperature controller 12 auto-controls, selecting the temperature control scope by temperature regulation switch 13 is 5 ℃～50 ℃, temperature controller 12 control temperature fluctuations are ± 0.5 ℃, and temperature homogeneity is ± 1 ℃ in the casing, but temperature controller 12 continuous workings.Biochemical cultivation case is also connected with external power source 9.
After Plant arrangement is good, during work, at first start the temperature controller of biochemical cultivation case, in order to keep the temperature inside the box constant, guarantee to test and under set temperature conditions (for example 20 ℃), carry out.And then the mobile phone radiation source is transferred to corresponding working frequency range and output power gear.Again C. Elegans Automatic Screening is put in radiating antenna around and start the mobile phone radiation source, in order to carry out the research of long-time, low dosage electromagnetic radiation and anti-electromagnetic-radiation medicine.
The electromagnetic radiation damage Effect Evaluation method of utilizing said apparatus to carry out, and utilize the method that the examination of anti-electromagnetic-radiation medicine is used, comprise the steps:
1, experiment condition is set;
2, the nutrient culture media of C. Elegans Automatic Screening being put into double dish by the experiment grouping is put on the cultivation platform again, cultivates under different electromagnetic radiation conditions;
3, in nutrient culture media, can add medicine to be evaluated, at times growth conditions of each group nematode be carried out image acquisition, respectively organize the grow difference of state of nematode;
4, estimate medicine for the protective effect of nematode electromagnetic radiation damage;
5, for normal experiment nematode of testing nematode and mutating strain series, can place it in this electromagnetic irradiating device, by the pattern of growing of observation analysis C. Elegans Automatic Screening, judge whether electromagnetic radiation has damage effect for C. Elegans Automatic Screening, and have which kind of damage effect.
Below estimate with the electromagnetic radiation biological effect of concrete C. Elegans Automatic Screening, and application example and the comparative example of protection medicine screening is described further.
Application examples 1: the electromagnetic radiation biological effect of C. Elegans Automatic Screening is estimated
1, material is prepared
1) device: electromagnetic radiation experimental provision of the present invention;
2) animal used as test: daf-16 (mu86) mutating strain series C. Elegans Automatic Screening, synchronization process, the L1 phase is inoculated and is placed on normal raising the in the experimental provision;
3) experiment grouping: blank group, contrast administration group, irradiation group, irradiation administration group; Medicine is plant polyose.
2, experimental technique
1) preparation contains common NGM nutrient culture media and the NGM nutrient culture media that contains medicine;
2) nutrient culture media is poured in the 3.5cm double dish, drying at room temperature is coated with bacterium (Escherichia coli OP50) two days later, and being coated with the bacterium amount is 140 μ l/ wares, uses after the ambient temperature overnight;
3) about 100 of the L1 phase nematode of synchronization being cultivated is divided into irradiation group, control group, medicine control group and medicine-irradiation group, different double dish are put in grouping, control group and medicine control group 20 ℃ of Continuous Cultivation to 48h, irradiation group and medicine-irradiation group at power be under the electromagnetic radiation condition of 2.23W 20 ℃ of Continuous Cultivation to 48h;
4) in 48h growth conditions of each group nematode is carried out image acquisition, respectively organize under the varying environment condition, the grow difference of state of nematode, and estimate medicine for the protective effect of nematode electromagnetic radiation damage.
3, interpretation of result:
As shown in Figure 3, the speed of growth of irradiation group (right side is two pictures up and down) nematode is fast than control group (left side is two pictures up and down), show as irradiation group nematode volume significantly greater than control group, the prompting electromagnetic radiation has damage C. Elegans Automatic Screening, can change the growth cycle of C. Elegans Automatic Screening; And medicine-irradiation group (bottom right picture) compares with irradiation group (upper right picture), and the nematode volume obviously diminishes, and shows that this medicine can effectively improve electromagnetic radiation to the damage effect of nematode.
This shows: device of the present invention can be estimated the biological effect of electromagnetic radiation effectively, reliably, and is used for the screening of anti-electromagnetic-radiation medicine.
Comparative example:
Whether can the biological effect of electromagnetic radiation be affected to some extent in order to analyze the comparative heat effect, the existing electromagnetic irradiating device (WB-I type) of use is studied by changing temperature (artificial adjustment change is also kept this temperature constant).The speed of growth and the environment temperature of nematode are closely related, and normal raising temperature is 16 ℃ of-25 ℃ of scopes.The N2 nematode is under 16 ℃, 20 ℃, the 25 ℃ different growth temperatures, and there is gap in the required time of each growth phase, such as the rate of development under 25 ℃ of conditions faster than 16 ℃ and 20 ℃ of temperature conditions.For the difference between differential growth environmental temperature effect and the galvanomagnetic effect, irradiation group that will be identical with application examples 1 and the C. Elegans Automatic Screening of medicine-irradiation group are put into the existing electromagnetic radiation generating means of WB-I type and are studied, and draw the different temperatures environment for the impact of C. Elegans Automatic Screening growth curve.21h (L2 phase) begins to carry out image acquisition after the nematode inoculation, and every interval 3h carries out once, and continuous collecting is to 70h (adult stage and begin ovulation).Each time point nematode health area (body size) is carried out graphical analysis, and (unit is pixel, pixel) draws growth curve according to the bulk area of average every nematode.The result as shown in Figure 4, as seen from image acquisition time point 21h, the nematode bulk area of 25 ℃ of ambient growth is obviously greater than 20 ℃ of groups, and gap between the two increases gradually.Behind about 72h, this moment, 20 ℃ of groups progressed into the peak period of ovulation, and bulk area increases sharply, and two groups of bulk area gaps are the trend of dwindling.114h after inoculating, this moment, 20 ℃ of groups nematode was in the middle and later periods of the onset of ovulation, and 25 ℃ of nematodes have approached the latter stage of ovulating, visible two suite lines cross substantially.This shows that nematode is in the speed of growth under the normal raising condition faster than 20 ℃ of growing under 25 ℃ of conditions.If the existing electromagnetic radiation generating means of the clear and definite reality of this result does not carry out strict temperature controlled words, will have a strong impact on the growth of C. Elegans Automatic Screening, thereby make C. Elegans Automatic Screening be subject to severe jamming because of the mutagenic result of electromagnetic radiation.Electromagnetic irradiating device of the present invention then can the strictly controlled environment temperature, obtains the result of stable electromagnetic radiation biological effect.
Further analyze the laying quantitative analysis result of visible 25 ℃ of temperature control group nematodes as shown in Figure 5, as seen use merely 25 ℃ of feeding environments and do not carry out under the electromagnetic radiation irradiation condition, the egg-laying season distributed number of laying eggs of nematode is different from normal group (20 ℃), and the quantity of namely laying eggs between 3 days egg-laying season is successively decreased successively.And the statistical result showed of the sum of laying eggs, the sum of laying eggs of 25 ℃ of temperature groups nematodes obviously is less than 20 ℃ of normal group, further specify because temperature is very significant on the impact of nematode, the temperature and existing electromagnetic irradiating device (such as the WB-I type) is difficult to control environment, usually work at ambient temperature, when being used for the research of electromagnetic radiation biological effect, obviously can't obtain stable, the reliable result of Special electromagnetic radiation experiments device of the present invention.
The above results shows that fully existing electromagnetic radiation generating means irradiation long-time, low dosage can affect the growth of C. Elegans Automatic Screening because of the environment temperature rising, thereby C. Elegans Automatic Screening is interfered because of the mutagenic result of electromagnetic radiation.
Application examples 2:
Irradiation group that will be identical with application examples 1 and the C. Elegans Automatic Screening of medicine-irradiation group are put into electromagnetic radiation generating means of the present invention, under uniform temp and power, implement irradiation, Continuous irradiation 70 hours is observed the impact that 1750MHz and 900MHz electromagnetic radiation are grown on nematode.21h (L2 phase) begins to carry out image acquisition after the nematode inoculation, and every interval 3h carries out once, and continuous collecting is to 70h (adult stage and begin ovulation).Each time point nematode health area (body size) is carried out graphical analysis, and (unit is pixel, pixel) draws growth curve according to the bulk area of average every nematode.1750MHz electromagnetic radiation and 900MHz electromagnetic radiation affect the result as shown in Figure 6 for the nematode growth curve, as seen 21h rises after the nematode inoculation, the bulk area of irradiation group nematode is greater than the normal group nematode, and along with the prolongation gap between the two of irradiation time increases gradually.After the normal group nematode enters the onset of ovulation (about 60h), this moment, three groups of nematodes all were in the onset of ovulation, and the bulk area gap of nematode is the trend of dwindling between irradiation group and the control group.Simultaneously, the growth curve of 1750MHz and two kinds of radiation groups of 900MHz nematode is consistent substantially, without significant difference.This shows, two kinds of electromagnetic radiation of the 1750MHz of L-band and 900MHz have all been played the effect that promotes to growing of C. Elegans Automatic Screening, normal development for C. Elegans Automatic Screening has produced significant impact, illustrates that simultaneously electromagnetic radiation generating means of the present invention can be used in temperature biological effect research controlled, low-power, long-time electromagnetic radiation.
Simultaneously, laying eggs is the important indicator that nematode grows, and the egg-laying season generally continues 3 days.1750MHz and 900MHz electromagnetic irradiation affect the result as shown in Figure 7 to what nematode laid eggs: the normal nematode quantity of laying eggs in 3 days egg-laying season is the trend of " middle many two are few ", and namely the 2nd day the quantity of laying eggs is maximum, takes second place in the 1st day, and the 3rd day minimum.And after using electromagnetic irradiating device of the present invention to make C. Elegans Automatic Screening accept electromagnetic irradiation, the distribution of the quantity of laying eggs is different from normal group, presents the trend of successively decreasing, and the quantity of laying eggs was maximum in namely the 1st day, and quantity was successively decreased in the 2nd day the 3rd day.After the quantity of will laying eggs is cumulative, as seen respectively organize sum that nematode lays eggs and control group without significant difference (P＞0.05), although the low-power of further specifying, long-time electromagnetic radiation do not affect for the quantity of laying eggs of C. Elegans Automatic Screening, can significantly change the pattern of laying eggs of C. Elegans Automatic Screening.
Application examples 3: the examination of anti-electromagnetic-radiation medicine
1. anemaran is to the electromagnetic protection effect of nematode
Use electromagnetic radiation experimental provision of the present invention, the examination anemaran is for the electromagnetic failure protective effect of nematode.Irradiation group and control group nematode grow at the anemaran NGM of concentration 0g/L, 5g/L, 10g/L, 15g/L culture plate respectively, (48h) carries out image acquisition to nematode in the L4 phase, estimate the state that grows of respectively organizing nematode, result such as Fig. 8, shown in Figure 9.As seen blank group nematode 48h is in the L4 phase, and irradiation group nematode has grown to the adult stage, and as seen ovulation on the double dish, the growth of irradiation group nematode significantly are accelerated in blank group.Increase along with anemaran concentration among the NGM, 5g/L-irradiation group, 10g/L-irradiation group nematode have no ovulation in 48h, but its bulk area is still respectively significantly greater than 5g/L-control group, 10g/L-control group, when the concentration of anemaran further increases to 15g/L, as seen 15g/L-control group nematode is not occurring on the basis of ovulation, and the blank group of its bulk area and 15g/L-nematode is without significant difference.In addition, the grow state of irradiation-administration group nematode under each administration concentration is without significant difference.This shows that the 15g/L anemaran has certain electromagnetic protection effect for C. Elegans Automatic Screening, and have no obvious toxic and side effect.Utilize this electromagnetic radiation experimental provision to test, can know that anemaran has protective effect for the electromagnetic failure of nematode, and determine that better drug concentration is 15g/L.
2. LBP-X is to the electromagnetic protection effect of nematode
Irradiation group and control group nematode grow at the LBP-X NGM of concentration 0g/L, 5g/L, 10g/L, 15g/L culture plate respectively, (48h) carries out image acquisition to nematode in the L4 phase, estimate the state that grows of respectively organizing nematode, result such as Figure 10, shown in Figure 11, as seen control group nematode 48h is in the L4 phase, and irradiation group nematode has grown to the adult stage, as seen ovulation on the double dish, and the growth of irradiation group nematode significantly is accelerated in control group.Along with the increase of LBP-X concentration among the NGM, 5g/L-irradiation group nematode has no ovulation in 48h, but its bulk area is still significantly greater than 5g/L-contrast and control group.When the concentration of anemaran increased to 10g/L, 10g/L-irradiation group nematode was not occurring on the basis of ovulation, and its bulk area and 10g/L-control group, control group nematode are without significant difference.When the concentration of LBP-X increased to 15g/L, the bulk area of 15g/L-irradiation group and 15g/L-control group nematode was significantly less than control group, and the growth of prompting nematode has been subject to inhibition, and the LBP-X of this dosage has toxic and side effect.This shows that the 10g/L LBP-X has the electromagnetic radiation protection effect to C. Elegans Automatic Screening, and have no obvious toxic and side effect.Utilize this electromagnetic radiation experimental provision to test, can know that LBP-X has protective effect for the electromagnetic failure of nematode, and determine that better drug concentration is 10g/L.
This shows; medicine grouping to be screened is joined in the nutrient culture media of C. Elegans Automatic Screening; use this device that C. Elegans Automatic Screening is shone; observation is under pharmaceutically-active condition; whether the situation of laying eggs according to C. Elegans Automatic Screening improves with growing; can draw the conclusion whether this medicine has the protective effect of anti-electromagnetic-radiation damage, and can determine optimum medicine concentration, illustrate that electromagnetic radiation generating means of the present invention can be used for the examination of anti-electromagnetic-radiation medicine.
Claims (10)
1. electromagnetic radiation experimental provision, comprise electromagnetic radiation dummy source and biochemical cultivation case two parts, all power by external power supply, it is characterized in that: described biochemical cultivation case has larger volume, the electromagnetic radiation dummy source is placed centre position within it, around this electromagnetic radiation dummy source transmitting terminal, establish for the cultivation platform of placing laboratory sample, and biochemical cultivation case is provided with temperature controller, temperature regulation switch and temperature and shows, temperature fluctuation is ± 0.5 ℃ in the control box, and temperature homogeneity is ± 1 ℃.
2. electromagnetic radiation experimental provision according to claim 1, it is characterized in that: described biochemical cultivation case is the constant incubator of 250L, and described electromagnetic radiation dummy source is the mobile phone radiation source, and overall dimensions are 210 * 190 * 80mm, and transmitting terminal is emitting antenna.
3. electromagnetic radiation experimental provision according to claim 2, it is characterized in that: described electromagnetic radiation dummy source is provided with 1750MHz and two kinds of transmission frequencies of 900MHz, and each frequency is respectively arranged with 0.1,0.2,0.2,0.6,0.8,1 six gear.
4. electromagnetic radiation experimental provision according to claim 3, it is characterized in that: described electromagnetic radiation dummy source comprises box body, frequency generator is housed in the box, the antenna of two kinds of frequencies of emission vertically is set on the box body, and the box body operation and control interface is established each self-corresponding frequency fork shift and power switch under frequency change-over switch, the two kinds of frequencies.
5. according to claim 1 to 4 arbitrary described electromagnetic radiation experimental provisions, it is characterized in that: described cultivation platform is the circular cylindrical cavity about high 2-5mm, described laboratory sample is double dish or the culture plate that C. Elegans Automatic Screening is housed, and double dish or culture plate place on the circular cylindrical cavity; Cultivation platform is a plurality of, be distributed in internal antenna around.
6. one kind is utilized the described electromagnetic radiation experimental provision of one of claim 1～5 to carry out electromagnetic radiation damage Effect Evaluation method, comprises the steps:
1) sets the temperature inside the box by temperature controller and temperature regulation switch, set electromagnetic radiation dummy source emissive power by the frequency fork shift;
2) laboratory sample is put in the double dish by grouping, double dish is put into the cultivation platform position of biochemical cultivation case, cultivate setting under the electromagnetic radiation condition;
3) cultivate to finish after, growth conditions of each group nematode is carried out image acquisition, respectively organize the grow difference of state of nematode, whether evaluation experimental group experiment condition has the electromagnetic radiation damage effect.
7. electromagnetic radiation damage Effect Evaluation method according to claim 6 is characterized in that: comprise C. Elegans Automatic Screening in the described laboratory sample.
8. method of utilizing the described electromagnetic radiation experimental provision of one of claim 1～5 to carry out the examination of anti-electromagnetic-radiation medicine may further comprise the steps:
1) medicine that will intend screening is mixed with the NGM culture plate of variable concentrations, bioorganism is grown grouping at this culture plate;
2) each group laboratory sample is placed in the described electromagnetic radiation experimental provision and implements electromagnetic radiation, observe the pattern of growing of respectively organizing bioorganism in the laboratory sample;
3) judge whether electromagnetic radiation has damage effect to bioorganism, and have which kind of damage effect;
4) respectively organize the pattern of growing of bioorganism, judge the variable concentrations medicine for the impact of bioorganism, examination goes out to have medicine and the drug concentration of anti-electromagnetic-radiation.
9. method according to claim 8, it is characterized in that: described bioorganism is normal strain or mutating strain series C. Elegans Automatic Screening, described view mode be image acquisition and image ratio; Described impact is to grow promotion and/or the state of laying eggs.
10. it is characterized in that according to claim 8 or 9 described methods: each organizes the medicine that laboratory sample comprises blank group, irradiation group, the medicine-control group of variant concentration, variant concentration-irradiation group.
Priority Applications (1)
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| CN110596154A (en) * | 2018-06-13 | 2019-12-20 | 绍兴邦财纺织科技有限公司 | System and method for detecting electromagnetic radiation shielding capability of textile |
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