CN102867560B - A kind of electromagnetic radiation experimental device and application thereof - Google Patents
A kind of electromagnetic radiation experimental device and application thereof Download PDFInfo
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Abstract
The present invention relates to a kind of electromagnetic radiation experimental device and the damage effect evaluation methodology utilizing this device to carry out and the method purposes in anti-electromagnetic-radiation medicine examination.This device includes electromagnetic radiation dummy source and biochemical cultivation case two parts, power each through external power supply, described biochemical cultivation case has larger volume, electromagnetic radiation dummy source places centre position within it, surrounding at this electromagnetic radiation dummy source transmitting terminal sets the cultivation platform for placing laboratory sample, and biochemical cultivation case is provided with temperature controller, temperature regulation switch and temperature and shows, in control chamber, temperature fluctuation is ± 0.5 DEG C, and temperature homogeneity is ± 1 DEG C.This device electromagnetic radiation source includes two kinds of output frequencies, and each frequency includes 6 kinds of gears, it may be achieved 12 kinds of outputs, can continuous operation more than one month, meet the normal growing conditions of bioorganism.The main uses of the present invention is in that to carry out under ensureing animal normal growing conditions the evaluation of electromagnetic radiation effect and the screening of anti-electromagnetic-radiation medicine.
Description
Technical field
The invention belongs to biological medicine health care correlative technology field, it is specifically related to a kind of electromagnetic radiation effect evaluating apparatus and the damage effect evaluation methodology utilizing this device to carry out, available Caenorhabditis elegans carries out the evaluation of electromagnetic radiation damage effect, and can be used for screening and the research and development of anti-electromagnetic-radiation new drug.
Background technology
Electromagnetic radiation has become a kind of new environomental pollution source.Electromagnetic radiation in people's living environment mostly is low dosage, persistence radiation, and its biological effect mostly is accumulative effect, and the harm of human health be can not be ignored.But the mechanism of electromagnetic radiation biological effect there is no final conclusion, one of its important reason is that shortage biological effect evaluating apparatus sensitive, effective and method.At present, multiple electromagnetic radiation generating means, it is available in laboratory carrying out the biological effect evaluation of system, but these devices often volume such as such as electromagnetic reverberation room, radar signal simulator etc. is relatively big and self heat production during work, not easily it is placed in conventional constant incubator, is also difficult to get rid of the ambient temperature impact on electromagnetic radiation related experiment result.Although the short time, high dose radiation condition under can ignore the environmental factors impact on experimental result to a certain extent, but for the radiation parameter of long-time, low dosage, experimental result is subject to the interference of external environment (temperature, humidity etc.) unavoidably, and existing electromagnetic radiation generating means does not all consider this interference factor.Therefore, in the urgent need to setting up the electromagnetic radiation effect evaluating apparatus that disclosure satisfy that laboratory animal normal habitat demand, it is particularly suited for the evaluation of electromagnetic radiation accumulative effect.
In the research in the past of electromagnetic radiation biological effect, in vivo model and external model are widely used, both complexity and unicity cause that obtained biological effect is difficult to normalization, therefore in the urgent need to setting up and development one galvanomagnetic-effect evaluation system more sensitive, effective, so as sensitive, stable, reliably assess biohazard and the mechanism of electromagnetic radiation, meet the urgent needs of electromagnetic radiation protection medical science.Caenorhabditis elegans is that a kind of operation is easy, biological effect assesses model organism easily, compared with the cell model conventional with electromagnetic radiation biological effect research and higher mammal, there is some superiority, be in particular in that the dependency of temperature, humidity, air and the condition of culture such as aseptic is caused that it is difficult to the electromagnetic pollution evaluation in actual environment by cell culture technology；And the complexity of higher mammal causes that utilizing the mammals such as mice, rat, rabbit to carry out the exposure of short-term electromagnetic environment is hardly visible clear and definite biological effect, and while carrying out long-term electromagnetic environment exposure assessment accumulative effect, the change of environmental factors often leads to be difficult to from experimental result to screen interfering of electromagnetic failure effect and environmental factors effect.Use C. Elegans Automatic Screening to carry out this type of research and then there is many-sided advantage, it it is a kind of idealized model biology that can evaluate electromagnetic radiation biological effect, there is raising simple (normal pressure, normal oxygen, 20 DEG C of conditions), breeding is easily (hermaphroditism, self-propagation), the advantages such as phenotype abundant (having abundant physical signs, behavioral indicator, learning and memory evaluation index etc.), are therefore highly suitable for the biological effect evaluation of electromagnetic radiation from environment and the screening of electromagnetic protection medicine.
Summary of the invention
For the problems referred to above, it is an object of the invention to set up a kind of simple and easy to do electromagnetic radiation experimental device that can be used for electromagnetic radiation damage effect is carried out objective evaluation, electromagnetic radiation can be given under normal growth state for the laboratory animal including C. Elegans Automatic Screening, thus realizing electromagnetic radiation damage Effect Evaluation method, and it is further used for the examination of anti-electromagnetic-radiation medicine.
For achieving the above object, the present invention takes techniques below scheme:
First aspect, the present invention provides a kind of electromagnetic radiation experimental device, including electromagnetic radiation dummy source and biochemical cultivation case two parts, power each through external power supply, it is characterized in that: described biochemical cultivation case has larger volume, electromagnetic radiation dummy source places centre position within it, surrounding at this electromagnetic radiation dummy source transmitting terminal sets the cultivation platform for placing laboratory sample, and biochemical cultivation case is provided with temperature controller, temperature regulation switch and temperature and shows, in control chamber, temperature fluctuation is ± 0.5 DEG C, and temperature homogeneity is ± 1 DEG C.
Described biochemical cultivation case is the constant incubator of 250L, and described electromagnetic radiation dummy source is cellular radiation source, and overall dimensions are 210 × 190 × 80mm, and transmitting terminal is transmitting antenna.
Described electromagnetic radiation dummy source is provided with two kinds of tranmitting frequencies of 1750MHz and 900MHz, and each frequency is respectively arranged with 0.1,0.2,0.2,0.6,0.8,1 six gears.
Described electromagnetic radiation dummy source includes box body, and box, built with frequency generator, box body is vertically arranged the antenna launching two kinds of frequencies, and box body operation and control interface sets each self-corresponding frequency fork shift and on and off switch under frequency error factor switch, two kinds of frequencies.
Described cultivation platform is the circular cylindrical cavity of high about 2-5mm, and described laboratory sample is the culture dish equipped with C. Elegans Automatic Screening or culture plate, culture dish or culture plate are placed on circular cylindrical cavity；Cultivation platform is multiple, is distributed in the surrounding of internal antenna.
Second aspect, the present invention provides one to utilize described electromagnetic radiation experimental device to carry out electromagnetic radiation damage Effect Evaluation method, comprises the steps:
1) set the temperature inside the box by temperature controller and temperature regulation switch, set electromagnetic radiation dummy source transmitting power by frequency fork shift；
2) laboratory sample is put in culture dish by packet, culture dish is put into the cultivation platform position of biochemical cultivation case, cultivate when setting electromagnetic radiation；
3) after cultivation terminates, the growth conditions of each group of nematicide being carried out image acquisition, respectively organize the difference of nematode growth developmental condition, whether evaluation experimental group experiment condition has electromagnetic radiation damage effect.
In the method, described laboratory sample includes C. Elegans Automatic Screening.
The third aspect, the present invention provides a kind of method utilizing described electromagnetic radiation experimental device to carry out anti-electromagnetic-radiation medicine examination, comprises the following steps:
1) medicine intending screening is configured to the NGM culture plate of variable concentrations, bioorganism is grown on this culture plate, packet；
2) each group of laboratory sample is placed in described electromagnetic radiation experimental device and implements electromagnetic radiation, observe the growth promoter pattern of bioorganism in each group of laboratory sample；
3) judge whether bioorganism is had damage effect by electromagnetic radiation, and which kind of damage effect there is；
4) the growth promoter pattern of bioorganism is respectively organized, it is judged that variable concentrations medicine is for the impact of bioorganism, and examination goes out to have medicine and the drug level of anti-electromagnetic-radiation.
Wherein, described bioorganism is normal strain or mutating strain series C. Elegans Automatic Screening, and described view mode is image acquisition and image compares；Described impact is grow to promote and/or state of laying eggs.
Each group laboratory sample includes the medicine-irradiation group of blank group, irradiation group, the medicine-matched group of variant concentration, variant concentration.
Due to the fact that and take above technical scheme, it has the advantage that 1, apparatus of the present invention temperature-control range is extensive, can meet the normal growth needs of animal, and electromagnetic radiation dummy source is light easy, power adjustable.2, evaluation methodology of the present invention is simple and easy to do, be suitable to the impact evaluating and can getting rid of completely the outside environmental elements such as ambient temperature of electromagnetic radiation biological effect.3, application apparatus and method of the present invention, can for the screening of anti-electromagnetic-radiation new drug with research and develop guiding acting on.
Accompanying drawing explanation
Fig. 1 is electromagnetic radiation dummy source schematic appearance；
Fig. 2 is the schematic diagram of constant incubator and electromagnetic radiation dummy source combination unit；
Fig. 3 is that C. Elegans Automatic Screening developmental state when each packet in electromagnetic radiation environment shows.
The growth curve of Fig. 4 Normal group (20 DEG C) and 25 DEG C of temperature matched group N2 nematicides
Nematicide is laid eggs the impact of distributed number and sum of laying eggs by Fig. 5 matched group and 25 DEG C of temperature conditions
The growth curve of N2 nematicide when Figure 61 750MHz and 900MHz electromagnetic radiation
Fig. 7 electromagnetic irradiation nematicide is laid eggs distributed number and lay eggs sum impact
The growth promoter state of nematicide after the anemaran effect 48h of Fig. 8 variable concentrations
Fig. 9 variable concentrations anemaran protected effect to electromagnetic radiation
The growth promoter state of nematicide after the lycium barbarum polysaccharide effect 48h of Figure 10 variable concentrations
Figure 11 variable concentrations lycium barbarum polysaccharide protected effect to electromagnetic radiation
Detailed description of the invention
In order to the impact of ambient temperature etc. that the C. Elegans Automatic Screening ensured in electromagnetic radiation process is in normal growth conditions and is not brought by electromagnetic radiation, the present invention adopts the mode that constant incubator combines with electromagnetic radiation source (cellular radiation source) to carry out electromagnetic radiation biological effect research.
For this, apparatus of the present invention are based on following design philosophy: 1, based on the thermally-stabilised principle of constant incubator and the second law of thermodynamics, when in the casing of constant incubator, local temperature rises, the temperature that can be cultured in case is balanced, and the factor such as the temperature-control performance of the balanced speed of local temperature and the volume in cellular radiation source, the volume of incubator and incubator is relevant.When incubator volume (being designed as 250L) is far longer than the volume in cellular radiation source, the local temperature near cellular radiation source can quickly be balanced.2, owing to the radiating bands that mobile phone uses in the market is 900MHz and 1800MHz etc., and the temperature that small size cellular radiation source causes rises and is easily balanced by constant incubator, thus devise containing the small handsets radiation source that two kinds of microwave frequencies (respectively 1750MHz and 900MHz), output are adjusting shift, its volume is 210 × 190 × 80mm, and whip antenna height is 100mm, diameter is 8mm；And each radiation frequency is divided into 6 grades, and respectively 0.1,0.2,0.4,0.6,0.8 and 1 grade, peak power output is in 1 file location, and its power is 2.23W, and minimum output power is in 0.1 file location, and its power is 0.008W.
Based on above-mentioned design philosophy, existing constant incubator is transformed by the present invention accordingly, machined a kind of can the small handsets radiation source of analogue mobile phone radiant power, it is placed in constant incubator and carries out electromagnetic radiation, thus avoiding the hot dry of generation in electromagnetic radiation process to disturb the electromagnetic radiation effect to C. Elegans Automatic Screening, it is more beneficial for carrying out the research of long-time, low dosage electromagnetic radiation damage mechanism and anti-electromagnetic-radiation medicine.
Below in conjunction with drawings and Examples, the present invention is described in detail.
Experimental provision of the present invention includes biochemical cultivation case and the electromagnetic radiation dummy source being located therein.
Electromagnetic radiation dummy source is the miniaturized radiation source (also referred to as cellular radiation source) of analogue mobile phone radiation frequency, adopt two kinds of tranmitting frequencies of 1750MHz and 900MHz, 6 gears (0.1,0.2,0.4,0.6,0.8,1) it are respectively arranged with under each frequency, can realizing the switching of 2 kinds of frequencies, 12 kinds of outputs altogether, its outward appearance operation and control interface is as shown in Figure 1.Shown in Fig. 2, dummy source includes box body 1, box body is of a size of 210 × 190 × 80mm, box is not built with frequency generator (drawing in figure), box body 1 is respectively perpendicular to cartridge upper surface the whip antenna 2,3 of two kinds of frequencies of 900MHz and 1750MHz is set, box body 1 operation and control interface arranges frequency error factor switch 4, each self-corresponding frequency fork shift 5,6 under two kinds of frequencies, and on and off switch 7.Electromagnetic radiation dummy source output and gear comparison are as shown in table 1.
Table 1: output and gear synopsis
Biochemical cultivation case selects volume to be the constant incubator of 250L, including a containment housing 8, in casing 8, bracing frame 15 is set and puts the box body 1 of dummy source for card, make box body 1 be in as far as possible the central area of casing 8 with ensure the electromagnetism wave energy of its transmitting in casing uniform；It is laid with multiple C. Elegans Automatic Screening cultivation platform 14 at box body 1 upper surface, this cultivation platform 14 is diameter 35mm, the circular cylindrical cavity of high about 2-5mm, for placing C. Elegans Automatic Screening culture dish (or culture plate), multiple cultivation platforms 14 are distributed in the surrounding of casing 8 internal antenna 2 and antenna 3, cultivation platform 14 makes have certain interval to be beneficial to gas circulation between electromagnetic radiation dummy source and culture dish or culture plate, it is to avoid C. Elegans Automatic Screening in culture dish is produced impact by the change of electromagnetic radiation dummy source self-temperature；The power line of electromagnetic radiation dummy source is connected through the Kong Eryu external power source 9 of casing 8 side.Casing 8 outer surface arranges on and off switch 10, temperature display 11, temperature controller 12 and temperature regulation switch 13.Temperature controller 12 is purchased from Hengfeng Medical Instruments Co., Ltd., Huangsih City of Hubei Province, model is SP-01 type, and there is adjustment temperature function, its temperature control main body is loaded in casing 8, and the temperature inside the box is by temperature controller 12 auto-control in setting value, and temperature controller 12 is connected with temperature regulation switch 13, temperature control is selected to range for 5 DEG C～50 DEG C by temperature regulation switch 13, temperature controller 12 controls temperature fluctuation and is ± 0.5 DEG C, and in casing, temperature homogeneity is ± 1 DEG C, and temperature controller 12 can continuous operation.Biochemical cultivation case is also connected with external power source 9.
After being arranged by device, during work, first start the temperature controller of biochemical cultivation case, in order to temperature constant in holding tank, it is ensured that experiment carries out under set temperature conditions (such as 20 DEG C).Then cellular radiation source is transferred to corresponding working frequency range and output gear again.C. Elegans Automatic Screening it is put in the surrounding of active antenna again and starts cellular radiation source, in order to carrying out the research of long-time, low dosage electromagnetic radiation and anti-electromagnetic-radiation medicine.
Utilize the electromagnetic radiation damage Effect Evaluation method that said apparatus carries out, and utilize the method that the examination of anti-electromagnetic-radiation medicine is applied, comprise the steps:
1, experiment condition sets；
2, C. Elegans Automatic Screening is empirically grouped in the culture medium putting into culture dish it is put into again on cultivation platform, cultivate when different electromagnetic radiation；
3, medicine to be evaluated can be added in the medium, at times the growth conditions of each group of nematicide be carried out image acquisition, respectively organize the difference of nematode growth developmental condition；
4, the medicine protective effect for nematicide electromagnetic radiation damage is evaluated；
5, for the experiment nematicide of normal experiment nematicide and mutating strain series, can place it in this electromagnetic irradiating device, by the growth promoter pattern of observation analysis C. Elegans Automatic Screening, it is judged that electromagnetic radiation is for whether C. Elegans Automatic Screening has damage effect, and has which kind of damage effect.
Evaluate with the electromagnetic radiation biological effect of concrete C. Elegans Automatic Screening below, and the application example and comparative example to protection medicine screening is described further.
Application examples 1: the electromagnetic radiation biological effect of C. Elegans Automatic Screening is evaluated
1, material prepares
1) device: electromagnetic radiation experimental device of the present invention；
2) laboratory animal: daf-16 (mu86) mutating strain series C. Elegans Automatic Screening, synchronization process, the L1 phase is inoculated to be placed in experimental provision and normally raises；
3) experiment packet: blank group, comparison administration group, irradiation group, irradiation administration group；Medicine is vegetable polysaccharides.
2, experimental technique
1) preparation is containing common NGM culture medium and the NGM culture medium containing medicine；
2) culture medium being poured in 3.5cm culture dish, drying at room temperature is coated with bacterium (escherichia coli OP50) two days later, and being coated with bacterium amount is 140 μ l/ wares, uses after ambient temperature overnight；
3) the L1 phase nematicide about 100 of synchronizing culture is divided into irradiation group, matched group, drug control group and medicine-irradiation group, packet is put in different culture dish, matched group and drug control group 20 DEG C be cultured to continuously 48h, irradiation group and medicine-irradiation group when the electromagnetic radiation that power is 2.23W 20 DEG C be cultured to 48h continuously；
4) in 48h, the growth conditions of each group of nematicide is carried out image acquisition, respectively organize the difference of nematicide growth promoter state under difficult environmental conditions, and evaluate the medicine protective effect for nematicide electromagnetic radiation damage.
3, interpretation of result:
As shown in Figure 3, the speed of growth relatively matched group (upper and lower two pictures in left side) of irradiation group (upper and lower two pictures in right side) nematicide is fast, showing as irradiation group nematicide volume and be noticeably greater than matched group, C. Elegans Automatic Screening is had damage by prompting electromagnetic radiation, can change the growth phase of C. Elegans Automatic Screening；And medicine-irradiation group (bottom right picture) compares with irradiation group (upper right picture), nematicide volume substantially diminishes, it was shown that this medicine can be effectively improved the electromagnetic radiation damage effect to nematicide.
As can be seen here: device of the present invention can evaluate the biological effect of electromagnetic radiation effectively, reliably, and is used for the screening of anti-electromagnetic-radiation medicine.
Comparative example:
In order to whether com-parison and analysis heat effect can affect the biological effect of electromagnetic radiation to some extent, existing electromagnetic irradiating device (WB-I type) is used to study by changing temperature (manually adjust change and maintain this temperature constant).The speed of growth of nematicide is closely related with ambient temperature, and normal raising temperature is 16 DEG C of-25 DEG C of scopes.N2 nematicide is under 16 DEG C, 20 DEG C, 25 DEG C different growth temperatures, and the time needed for each growth stage exists gap, if the rate of development under 25 DEG C of conditions is faster than 16 DEG C with 20 DEG C of temperature conditions.For the difference between differential growth environmental temperature effect and galvanomagnetic-effect, the C. Elegans Automatic Screening of the irradiation group identical with application examples 1 and medicine-irradiation group is put in the existing electromagnetic radiation generating means of WB-I type and study, draw the impact for C. Elegans Automatic Screening growth curve of the different temperatures environment.After nematicide inoculation, 21h (L2 phase) proceeds by image acquisition, carries out once at interval of 3h, and continuous collecting is to 70h (adult stage and start ovulation).Each time point nematode body area (bodysize) is carried out graphical analysis, draws growth curve according to the bulk area (unit is pixel, pixel) of on average every nematicide.Result is as shown in Figure 4, it is seen that from image acquisition time point 21h, the nematicide bulk area of 25 DEG C of ambient growth is significantly greater than 20 DEG C of groups, and gap between the two is gradually increased.After about 72h, now 20 DEG C of groups progress into the peak period of ovulation, and bulk area increases sharply, and two groups of bulk area gaps are in the trend reduced.114h after inoculation, now 20 DEG C of group nematicides are in the middle and late stage of the onset of ovulation, and 25 DEG C of nematicides are already close to ovulation latter stage, it is seen that two suite lines cross substantially.As can be seen here, nematicide growth promoter under 25 DEG C of conditions is faster than the speed of growth under 20 DEG C of normal rearing conditions.If the existing electromagnetic radiation generating means of this result clearly reality does not carry out strict temperature controlled words, it will have a strong impact on the growth of C. Elegans Automatic Screening, so that the result that C. Elegans Automatic Screening produces to change because of electromagnetic radiation is heavily disturbed.The electromagnetic irradiating device of the present invention then can strictly controlled environment temperature, it is thus achieved that the result of stable electromagnetic radiation biological effect.
Analyze the laying quantitative analysis result of visible 25 DEG C of temperature matched group nematicides further as shown in Figure 5,25 DEG C of feeding environments of visible simple use are without when carrying out electromagnetic radiation irradiation, the distributed number of laying eggs of egg-laying season nematicide is different from normal group (20 DEG C), and namely the period laying amount of laying eggs of 3 days is successively decreased successively.And the statistical result showed of sum of laying eggs, the sum of laying eggs of 25 DEG C of temperature group nematicides is considerably less than 20 DEG C of normal group, further illustrate owing to the impact of nematicide is very significant by temperature, and existing electromagnetic irradiating device (such as WB-I type) is difficult to control to ambient temperature, generally work at ambient temperature, when for electromagnetic radiation biological effect research, it is clear that stable, the reliable result of the Special electromagnetic radiation experiments device of the present invention cannot be obtained.
The above results fully shows that existing electromagnetic radiation generating means is long-time, the irradiation of low dosage can affect the growth of C. Elegans Automatic Screening because of ambient temperature rising, so that the result that C. Elegans Automatic Screening produces to change because of electromagnetic radiation is interfered.
Application examples 2:
The C. Elegans Automatic Screening of the irradiation group identical with application examples 1 and medicine-irradiation group is put in electromagnetic radiation generating means of the present invention, implement to irradiate at identical temperature and power, Continuous irradiation 70 hours, observes the impact that nematode growth is grown by 1750MHz and 900MHz electromagnetic radiation.After nematicide inoculation, 21h (L2 phase) proceeds by image acquisition, carries out once at interval of 3h, and continuous collecting is to 70h (adult stage and start ovulation).Each time point nematode body area (bodysize) is carried out graphical analysis, draws growth curve according to the bulk area (unit is pixel, pixel) of on average every nematicide.1750MHz electromagnetic radiation and 900MHz electromagnetic radiation affect result as shown in Figure 6 for nematode growth curve, after the inoculation of visible nematicide, 21h rises, the bulk area of irradiation group nematicide is more than normal group nematicide, and along with the prolongation gap between the two of irradiation time is gradually increased.Normal group nematicide enters (about 60h) after the onset of ovulation, and now three groups of nematicides are in the onset of ovulation, and between irradiation group and matched group, the bulk area gap of nematicide is in reducing trend.Meanwhile, the growth curve of two kinds of radiation group nematicides of 1750MHz and 900MHz keeps consistent substantially, without significant difference.As can be seen here, the growth promoter of C. Elegans Automatic Screening is all served the effect of promotion by two kinds of electromagnetic radiation of 1750MHz and the 900MHz of L-band, normal development for C. Elegans Automatic Screening creates significant impact, illustrate simultaneously the electromagnetic radiation generating means of the present invention can be used in temperature-controllable, low-power, long-time electromagnetic radiation biological effect research.
Meanwhile, lay eggs be nematode growth grow important indicator, the egg-laying season generally continue 3 days.What nematicide was laid eggs by 1750MHz and 900MHz electromagnetic irradiation affects result as shown in Figure 7: normal nematicide lays eggs the quantity trend in " middle many two are few " in the egg-laying season of 3 days, and namely the quantity of laying eggs of the 2nd day is maximum, within the 1st day, takes second place, and the 3rd day minimum.And after the electromagnetic irradiating device using the present invention makes C. Elegans Automatic Screening accept electromagnetic irradiation, the distribution of quantity of laying eggs is different from normal group, presents the trend successively decreased, quantity of namely laying eggs for the 1st day is maximum, and within the 2nd day the 3rd day, quantity is successively decreased.After quantity of laying eggs is cumulative, the sum that visible each group of nematicide lays eggs and matched group are without significant difference (P > 0.05), although the low-power of further illustrating, long-time electromagnetic radiation do not affect for the quantity of laying eggs of C. Elegans Automatic Screening, but can significantly change the pattern of laying eggs of C. Elegans Automatic Screening.
Application examples 3: the examination of anti-electromagnetic-radiation medicine
1. the anemaran electromagnetic protection effect to nematicide
The electromagnetic radiation experimental device of the application present invention, examination anemaran is for the electromagnetic failure protective effect of nematicide.Irradiation group and matched group nematicide respectively concentration 0g/L, 5g/L, 10g/L, 15g/L anemaran NGM culture plate on growth, in the L4 phase (48h), nematicide is carried out image acquisition, evaluating the growth promoter state of each group of nematicide, result is as shown in Figure 8, Figure 9.Visible blank group nematicide 48h is in the L4 phase, and irradiation group nematicide has grown to the adult stage, and culture dish is ovulated as seen, and the growth of irradiation group nematicide substantially speeds up in blank group.Along with the increase of anemaran concentration in NGM, 5g/L-irradiation group, 10g/L-irradiation group nematicide have no ovulation in 48h, but its bulk area is still noticeably greater than 5g/L-matched group, 10g/L-matched group respectively, when the concentration of anemaran is further increased to 15g/L, visible 15g/L-matched group nematicide is not occurring that, on the basis of ovulation, its bulk area and 15g/L-blank group nematicide are without significant difference.Additionally, the growth promoter state that irradiation-administration group nematicide is under each administration concentration is without significant difference.As can be seen here, 15g/L anemaran has certain electromagnetic protection effect for C. Elegans Automatic Screening, and has no obvious toxic and side effects.This electromagnetic radiation experimental device is utilized to test, it is possible to know that anemaran has protective effect for the electromagnetic failure of nematicide, and determine that preferably drug level is 15g/L.
2. the lycium barbarum polysaccharide electromagnetic protection effect to nematicide
Irradiation group and matched group nematicide respectively concentration 0g/L, 5g/L, 10g/L, 15g/L lycium barbarum polysaccharide NGM culture plate on growth, in the L4 phase (48h), nematicide is carried out image acquisition, evaluate the growth promoter state of each group of nematicide, result is as shown in Figure 10, Figure 11, visible matched group nematicide 48h is in the L4 phase, and irradiation group nematicide has grown to the adult stage, culture dish being ovulated as seen, the growth of irradiation group nematicide substantially speeds up in matched group.Along with the increase of lycium barbarum polysaccharide concentration in NGM, 5g/L-irradiation group nematicide has no ovulation in 48h, but its bulk area is still noticeably greater than 5g/L-comparison and matched group.When the concentration of anemaran increases to 10g/L, 10g/L-irradiation group nematicide is not occurring that, on the basis of ovulation, its bulk area and 10g/L-matched group, matched group nematicide are without significant difference.When the concentration of lycium barbarum polysaccharide increases to 15g/L, the bulk area of 15g/L-irradiation group and 15g/L-matched group nematicide is significantly less than matched group, and the growth of prompting nematicide is inhibited, and the lycium barbarum polysaccharide of this dosage has toxic and side effects.As can be seen here, C. Elegans Automatic Screening is had electromagnetic radiation protection effect by 10g/L lycium barbarum polysaccharide, and has no obvious toxic and side effects.This electromagnetic radiation experimental device is utilized to test, it is possible to know that lycium barbarum polysaccharide has protective effect for the electromagnetic failure of nematicide, and determine that preferably drug level is 10g/L.
As can be seen here; medicine to be screened packet is joined in the culture medium of C. Elegans Automatic Screening; use this device that C. Elegans Automatic Screening is irradiated; observe under pharmaceutically-active condition; whether situation of laying eggs and growth promoter according to C. Elegans Automatic Screening improve; can show whether this medicine has the conclusion of the protective effect of anti-electromagnetic-radiation damage, it is possible to determine optimum medicine concentration, illustrate that the electromagnetic radiation generating means of the present invention can be used for the examination of anti-electromagnetic-radiation medicine.
Claims (6)
1. an electromagnetic radiation experimental device, including electromagnetic radiation dummy source and biochemical cultivation case two parts, power each through external power supply, it is characterized in that: electromagnetic radiation dummy source is placed in biochemical cultivation case centre position, surrounding at this electromagnetic radiation dummy source transmitting terminal sets the cultivation platform for placing laboratory sample, and biochemical cultivation case is provided with temperature controller, temperature regulation switch and temperature and shows, in control chamber, temperature fluctuation is ± 0.5 DEG C, and temperature homogeneity is ± 1 DEG C；Described biochemical cultivation case is the constant incubator of 250L, and described electromagnetic radiation dummy source is cellular radiation source, and overall dimensions are 210 × 190 × 80mm, and transmitting terminal is transmitting antenna.
2. electromagnetic radiation experimental device according to claim 1, it is characterised in that: described electromagnetic radiation dummy source is provided with two kinds of tranmitting frequencies of 1750MHz and 900MHz, and each frequency is respectively arranged with 0.1,0.2,0.4,0.6,0.8,1 six gears.
3. electromagnetic radiation experimental device according to claim 2, it is characterized in that: described electromagnetic radiation dummy source includes box body, box is built with frequency generator, being vertically arranged the antenna launching two kinds of frequencies on box body, box body operation and control interface sets each self-corresponding frequency fork shift and on and off switch under frequency error factor switch, two kinds of frequencies.
4. electromagnetic radiation experimental device according to claim 3, it is characterised in that: described cultivation platform is the circular cylindrical cavity of high 2-5mm, and described laboratory sample is the culture dish equipped with C. Elegans Automatic Screening or culture plate, culture dish or culture plate are placed on circular cylindrical cavity；Cultivation platform is multiple, is distributed in the surrounding of antenna.
5. utilize the electromagnetic radiation experimental device described in claim 3 or 4 to carry out an electromagnetic radiation damage Effect Evaluation method, comprise the steps:
1) set the temperature inside the box by temperature controller and temperature regulation switch, set electromagnetic radiation dummy source transmitting power by frequency fork shift；
2) laboratory sample is put in culture dish by packet, culture dish is put into the cultivation platform position of biochemical cultivation case, cultivate when setting electromagnetic radiation；
3) after cultivation terminates, the growth conditions of each group of nematicide being carried out image acquisition, respectively organize the difference of nematode growth developmental condition, whether evaluation experimental group experiment condition has electromagnetic radiation damage effect；
Described laboratory sample is C. Elegans Automatic Screening.
6. the method that the electromagnetic radiation experimental device utilizing one of claim 1～4 described carries out anti-electromagnetic-radiation medicine examination, comprises the following steps:
1) medicine intending screening is configured to the NGM culture plate of variable concentrations, bioorganism is grown on this culture plate, packet；
2) each group of laboratory sample is placed in described electromagnetic radiation experimental device and implements electromagnetic radiation, observe the growth promoter pattern of bioorganism in each group of laboratory sample；
3) by the difference of the growth promoter pattern of bioorganism in respectively group laboratory sample, it is judged that whether bioorganism is had damage effect by electromagnetic radiation, and has which kind of damage effect；
4) the growth promoter pattern of bioorganism is respectively organized, it is judged that variable concentrations medicine is for the impact of bioorganism, and examination goes out to have medicine and the drug level of anti-electromagnetic-radiation；
Described bioorganism is normal strain or mutating strain series C. Elegans Automatic Screening, and described view mode is image acquisition and image compares；Described impact is grow to promote and/or state of laying eggs；
Each group laboratory sample includes the medicine-irradiation group of blank group, irradiation group, the medicine-matched group of variant concentration, variant concentration.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201210233298.1A CN102867560B (en) | 2011-07-05 | 2012-07-05 | A kind of electromagnetic radiation experimental device and application thereof |
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| Application Number | Priority Date | Filing Date | Title |
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| CN103489494B (en) * | 2013-09-29 | 2015-09-23 | 汇佳生物仪器（上海）有限公司 | With the x-ray irradiation protective housing of radiation proof illumination and camera monitoring device |
| CN107167671A (en) * | 2017-06-13 | 2017-09-15 | 华东师范大学 | Animal electromagnetic radiation test device based on monitoring mobile phone |
| CN108519220B (en) * | 2018-02-28 | 2020-03-24 | 西安空间无线电技术研究所 | Antenna rib high accuracy thermal stability testing arrangement |
| CN110596154A (en) * | 2018-06-13 | 2019-12-20 | 绍兴邦财纺织科技有限公司 | System and method for detecting electromagnetic radiation shielding capability of textile |
| GB2579224A (en) * | 2018-11-26 | 2020-06-17 | Zyzzle Ltd | Apparatus for producing an improved animal population |
| CN110837018A (en) * | 2019-11-26 | 2020-02-25 | 北京智慧云测信息技术有限公司 | Anti-electromagnetic interference detection system of intelligent door lock |
| CN113340921B (en) * | 2021-02-01 | 2022-11-01 | 中国人民解放军军事科学院军事医学研究院 | Quantitative evaluation method and evaluation system for electromagnetic radiation damage and electromagnetic radiation biological effect |
| CN114002513A (en) * | 2021-11-05 | 2022-02-01 | 西北师范大学 | High-low electromagnetic field interactive radiation simulation device and use method thereof |
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