CN102784205B - Externally-applied medicine for treating rheumatoid diseases and preparation method thereof - Google Patents
Externally-applied medicine for treating rheumatoid diseases and preparation method thereof Download PDFInfo
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Abstract
The invention provides an externally-applied medicine for treating rheumatoid diseases and a preparation method thereof. The preparation method comprises the following steps: respectively soaking giantleaf ardisia rhizome, tinospora sinensis merr, paramichelia baillonii, paris polyphylla, smallflower galinsoga flower and philippine flemingia root traditional chinese medicinal materials into boiled rice vinegar; inoculating the materials to bacillus aceticus (Acetobactersp.) GLYSM66 CCTCC NO: M2011238 and fermentating for different fermentation time; and blending fermentation liquids so as to obtain the externally-applied medicine. The externally-applied medicine has the advantages of not only relieving pains of patients of rheumatoid arthritis, and but also being good in treatment effect, low in cost, simple in preparation method and free from toxic and side effects.
Description
Technical field
The present invention relates to application compress, specifically a kind of rheumatoid application compress and preparation method thereof for the treatment of.
Background technology
Rheumatoid claims again rheumatoid arthritis, and the pathological changes outside chronic, symmetry, many synovial jointss inflammation and joint of take is main clinical manifestation, belongs to autoimmune inflammatory diseases.Have in early days joint red and swollen heat pain and dysfunction, can there is stiff deformity in various degree in joint late period, and with the atrophy of bone and bones flesh, very easily disable.At present, rheumatoid treatment has two kinds of doctor trained in Western medicine and the traditional Chinese medical science, and doctor trained in Western medicine is mainly pain management, naturopathy, and closed treating etc., this all can only relief of symptoms, and side effect is large; The traditional Chinese medical science think this disease be meridian stasis stagnant extremely, treat this disease and should focus on the function that regulates the vital organs of the human body.Because the processing procedure of most of Chinese medicines is by boiling, the effective ingredient in Chinese crude drug is extracted by the method for decocting in water.But this method has following shortcoming: 1, owing to boiling time, temperature, pressure, can not unify, the effective ingredient in Chinese crude drug is difficult to be extracted completely; 2, boil in process temperature very high, likely destroyed the effective ingredient of those heat sensitivitys; 3, by boiling merely the Chinese medicine ingredients that lixiviate goes out, be the intermediate of effective ingredient mostly, must transform by the probiotic bacteria in human body, finally become the effective ingredient working, enter blood, because human body probiotic bacteria situation is different, thereby produce the situation such as slow or ineffective that takes effect, therapeutic effect is unsatisfactory, exploitation low toxicity, resisting rheumatoid disease new drug is the vital task of rheumatoid disease control efficiently.
Summary of the invention
For the deficiencies in the prior art, the invention provides a kind of pain relieving fast, can channel recuperating, good effect, the rheumatoid application compress for the treatment of that have no side effect again.
Another object of the present invention is to provide the preparation method of these application compress.
The technical scheme that realizes the object of the invention is:
A kind ofly treat rheumatoid application compress, it is to be immersed in respectively in boiling cooling rice vinegar by Rhizoma Ardisiae Gigantifoliae, Herba Lycopodii, Radix Polygalae Wattersii, Rhizoma Paridis, Flos Galinsogae Parviflorae, Radix Flemingiae Philippinensis Chinese crude drug, inoculate acetobacter, when different fermentations after indirect fermentation, then its fermentation liquid is blent and made.
Described acetobacter is acetobacter (Acetobacter sp.) GLYSM66 CCTCC NO:M2011238, be called for short acetobacter GLYSM66, that the inventor screens from common acetobacter, on July 5th, 2011, being deposited in Wuhan University's Chinese Typical Representative culture collection center (is called for short: CCTCC), deposit number is CCTCC NO:M2011238.This acetobacter has the function of degraded several kinds of Chinese medicinal materials or composite Chinese crude drug.
The preparation method of application compress of the present invention, comprises the steps:
(1) Chinese crude drug Rhizoma Ardisiae Gigantifoliae, Herba Lycopodii, Radix Polygalae Wattersii, Rhizoma Paridis, Flos Galinsogae Parviflorae, Radix Flemingiae Philippinensis were pulverized respectively to 100 orders, and autoclaving 30 minutes, standby;
(2) rice vinegar is boiled with warm fire, naturally cool to room temperature, standby;
(3) six kinds of Chinese crude drugs step (1) being processed are immersed in respectively in the rice vinegar after step (2) sterilization, and solid-liquid ratio is 1 ︰ (8-10);
(4) by acetobacter GLYSM66 with after LB culture medium amplification culture, be inoculated into respectively in each medical material soak, its inoculum concentration is: medical material soaks the ︰ 1 of the volume ratio of Ye ︰ strain=(20-50);
(5) ferment at normal temperatures, fermentation time is respectively: Rhizoma Ardisiae Gigantifoliae 145-155 days, Herba Lycopodii 115-130 days, Radix Polygalae Wattersii 85-100 days, Rhizoma Paridis 175-185 days, Flos Galinsogae Parviflorae 145-155 days, Radix Flemingiae Philippinensis 115-130 days;
(6) blend: get above-mentioned each medicine fermentation liquid and blend by following weight part ratio: the colored ︰ of osmanthus, the wide muscle rattan ︰ mountain of tire ︰ flower ︰ seven leaf that gallops along on horseback is to ︰ (3-5) ︰ (3-5) ︰ (0.4-0.6) ︰ (1-3) ︰ (2-4) of the colored ︰ Radix Flemingiae Philippinensis of sun=(2-4);
(7) seal up for safekeeping again after blending 3 months above.
The using method of this medicine has two kinds, can be with wherein a kind of, and also can two kinds of combinations:
First method is:
(1) first take a shower, after patient's pore is fully opened, with medicinal liquid fumigation 30 minutes to 1 hour;
(2) put on the clothes that medication immersion was steeped, parcel health;
(3) with infrared ray, toast whole body;
(4) carry out channels and network vessels regulation.
It within 10 days, is a course for the treatment of.
Second method is: external application, with absorbent paper, suction medicinal liquid, and apply at rheumatoid position, external preservative film encases, and with adhesive tape, fixes, and at least apply 6 hours every day, within 7 days, is a course for the treatment of.
In these application compress, the pharmacological action of each medical material is according to the record of the books such as < < Chinese medicine voluminous dictionary > > and < < whole nation Chinese herbal medicine compilation > >: Flos Galinsogae Parviflorae is the flower of feverfew Herba galinsogae parviflorae, has liver heat removing and eyesight improving, Rhizoma Paridis, another name: Typhonium divaricatum (L.) Decne, ferrum lamp lotus, have heat-clearing and toxic substances removing, an eliminating carbuncle of invigorating blood circulation, expelling wind and removing dampness sore, Radix Flemingiae Philippinensis is the root of the climing property of leguminous plant Radix Flemingiae Philippinensis, has the function of wind-damp dispelling, pain relieving, pathogenic wind expelling and blood circulation promoting, Rhizoma Ardisiae Gigantifoliae is Myrsinacea plant, has and dispels the wind, reducing swelling and alleviating pain, Radix Polygalae Wattersii is the become mildewed root of Radix Polygalae of milk wort, leaf or bark, there is medicine powder for relaxing muscle blood, promoting blood circulation and detoxication, Herba Lycopodii is Tinospora sinensis, another name: relaxing muscles and tendons rattan, Caulis Tinosporae, there is expelling wind and removing dampness, relaxing muscles and tendons and activating QI and blood in the collateral, the function of reducing swelling and alleviating pain, these Chinese crude drugs are after acetobacter fermentation of the present invention, make Chinese crude drug decomposition and inversion, effective ingredient is fully discharged, strengthened the property of medicine, existence and the strong acid environment of while acetobacter, strengthened the non-oxidizability of effective ingredient, there is stronger blood circulation promoting and blood stasis dispelling, relaxing muscles and tendons and activating QI and blood in the collateral, the dehumidifying of Eradicates wind, the function of circulation of qi promoting anti-inflammatory analgetic.Result in clinical application shows, this medicine treatment rheumatoid effect is remarkable, and medical expense is low, and preparation method is simple, has no side effect again.
Clinical this medicine of use treatment rheumatoid 15 examples, significant curative effect 13 examples wherein, effective 2 examples, and skin is had no side effect.
The specific embodiment
The present invention is described by the following examples.
In the present embodiment, acetobacter GLYSM66 used is that screening technique by comprising the steps obtains:
(1) get Flos Galinsogae Parviflorae, Semen Fagopyri Esculenti root, very heavy group, YIDUOYUN or five kinds of Chinese crude drugs of Rhizoma Paridis, pulverize, cross 100 mesh sieves, autoclaving 30 minutes respectively afterwards, five kinds of Chinese crude drug mixed in equal amounts or deposit separately standby;
(2) use soaking in rice vinegar medical material, the mass volume ratio (Gong Jin ︰ liter of medical material and rice vinegar)=1 ︰ (5-10), soaking temperature 20-35 ℃, the time that soaking fermentation is different;
(3) get the fermentation liquid of different fermentations time, measure effective ingredient--total flavones and total saponins, simultaneously through 1000 power microscope microscopies, total flavones and total saponin content in the sample that relatively sample of five kinds of Chinese crude drug mixed in equal amounts and five kinds of Chinese crude drugs are single, found that: biased sample not only total flavones and total saponin content high, and in fermentation liquid, only have single microorganism; Microbe species in the latter's liquid is many.The microorganism of biased sample is carried out to separation screening, and successive transfer culture, obtains the acetobacter of the several kinds of Chinese medicinal materials of degrading of the present invention.
The amplification culture of acetobacter described in the present embodiment LB culture medium used is by sodium chloride 10g, peptone 10g, and yeast extract 5g adds water and is settled to 1L and makes.
Embodiment 1
A preparation method for the treatment of rheumatoid application compress, comprises the steps:
(1) Chinese crude drug Rhizoma Ardisiae Gigantifoliae, Herba Lycopodii, Radix Polygalae Wattersii, Rhizoma Paridis, Flos Galinsogae Parviflorae, Radix Flemingiae Philippinensis were pulverized respectively to 100 orders, and autoclaving 30 minutes, standby;
(2) rice vinegar is boiled with warm fire, naturally cool to room temperature, standby;
(3) five kinds of Chinese crude drugs step (1) being processed are immersed in respectively in the rice vinegar after step (2) sterilization, and solid-liquid ratio is 1 ︰ (8-10);
(4) by acetobacter GLYSM66 with after LB culture medium amplification culture, be inoculated into respectively in each medical material soak, its inoculum concentration is: medical material soaks the ︰ 1 of the volume ratio of Ye ︰ strain=(20-50);
(5) ferment at normal temperatures, fermentation time is respectively: Rhizoma Ardisiae Gigantifoliae 150 days, Herba Lycopodii 120 days, Radix Polygalae Wattersii 90 days, Rhizoma Paridis 180 days, Flos Galinsogae Parviflorae 150 days, Radix Flemingiae Philippinensis 120 days;
(6) blend: the weight part ratio that each medicine fermentation liquid is blent (kilogram) being: the Tai ︰ Kuan Jin Teng ︰ Shan Gui Hua ︰ Qi leaf Yi Zhi Hua ︰ that gallops along on horseback is Xiang Yang Hua ︰ Radix Flemingiae Philippinensis=3 ︰ 4 ︰ 4 ︰ 0.5 ︰ 2 ︰ 3;
(7) seal up for safekeeping again after blending and within 90 days, make Compound Chinese Herbs for Fatigue-Resistance of Animal.
Embodiment 2
According to the method step of embodiment 1, carry out, different: the weight part ratio that described each medicine fermentation liquid of step (6) is blent (kilogram) being: the Tai ︰ Kuan Jin Teng ︰ Shan Gui Hua ︰ Qi leaf Yi Zhi Hua ︰ that gallops along on horseback, Xiang Yang Hua ︰ Radix Flemingiae Philippinensis=2.5 ︰ 3.5 ︰ .3.5 ︰ 0.4 ︰ 1.5 ︰ 2.5, seals up for safekeeping after blending 110 days again.
Embodiment 3
Apply this Compound Chinese Herbs for Fatigue-Resistance of Animal treatment hand rheumatoid effect example:
1, patient: Lee, man, 62 years old, people from hazard prevention, suffer from rheumatoid more than 10 year, symptom before treatment: both hands elbow joint bending, can not stretch normal amplitude, the appearance pain point that whole body can be indefinite, people is thin as bavin, after nursing one's health with the treatment of this medicine, pain spot and the pain degree of whole body reduce gradually, recovery from illness afterwards in 60 days.
2, patient: Lee, female, 33 years old, people from Pingyue County, hazard prevention, is engaged in agriculture, before treatment, both hands are from joints of hand to all enlargements of articulations digitorum manus, and the ankle arthrosis of both feet is to instep enlargement, and difficulty is slept round the clock, according to me, stating is that to live is no better than to die, skinny and wizened, the collapse of giving sb. a hard time, and whole body is frequent indefinite pain spot also, through treatment, the drug of topical application is after 6 hours, and pain reduces gradually, continuous use, nurses one's health recovery from illness in 45 days.
Claims (3)
1. treat rheumatoid application compress for one kind, it is characterized in that: it is to be immersed in respectively in the rice vinegar through boiling by Rhizoma Ardisiae Gigantifoliae, Herba Lycopodii, Radix Polygalae Wattersii, Rhizoma Paridis, Flos Galinsogae Parviflorae, Radix Flemingiae Philippinensis Chinese crude drug, inoculate acetobacter, when different fermentations after indirect fermentation, again its fermentation liquid is blent and made, wherein:
Described acetobacter is acetobacter (Acetobacter sp.) GLYSM66 CCTCC NO:M2011238;
The solid-liquid ratio of described each medical material and rice vinegar is 1 ︰ (8-10);
Described fermentation time is respectively: Rhizoma Ardisiae Gigantifoliae 145-155 days, Herba Lycopodii 115-130 days, Radix Polygalae Wattersii 85-100 days, Rhizoma Paridis 175-185 days, Flos Galinsogae Parviflorae 145-155 days, Radix Flemingiae Philippinensis 115-130 days;
The weight part ratio that described each fermentation liquid is blent is: the Tai ︰ Kuan Jin Teng ︰ Shan Gui Hua ︰ Qi leaf Yi Zhi Hua ︰ that gallops along on horseback is Xiang ︰ (3-5) ︰ (3-5) ︰ (0.4-0.6) ︰ (the 1-3) ︰ (2-4) of Yang Hua ︰ Radix Flemingiae Philippinensis=(2-4).
2. a preparation method for the treatment of rheumatoid application compress, is characterized in that: comprise the steps:
(1) Chinese crude drug Rhizoma Ardisiae Gigantifoliae, Herba Lycopodii, Radix Polygalae Wattersii, Rhizoma Paridis, Flos Galinsogae Parviflorae, Radix Flemingiae Philippinensis were pulverized respectively to 100 orders, and autoclaving 30 minutes, standby;
(2) rice vinegar is boiled with warm fire, naturally cool to room temperature, standby;
(3) five kinds of Chinese crude drugs step (1) being handled well are immersed in respectively in the rice vinegar after step (2) sterilization, and solid-liquid ratio is 1 ︰ (8-10);
(4) by acetobacter GLYSM66 with after LB culture medium amplification culture, be inoculated into respectively in each medical material soak, its inoculum concentration is: medical material soaks the ︰ 1 of the volume ratio of Ye ︰ strain=(20-50);
(5) ferment at normal temperatures, fermentation time is respectively: Rhizoma Ardisiae Gigantifoliae 145-155 days, Herba Lycopodii 115-130 days, Radix Polygalae Wattersii 85-100 days, Rhizoma Paridis 175-185 days, Flos Galinsogae Parviflorae 145-155 days, Radix Flemingiae Philippinensis 115-130 days;
(6) blend: get above-mentioned each medicine fermentation liquid and blend, its weight part ratio is: the Tai ︰ Kuan Jin Teng ︰ Shan Gui Hua ︰ Qi leaf Yi Zhi Hua ︰ that gallops along on horseback is Xiang ︰ (3-5) ︰ (3-5) ︰ (0.4-0.6) ︰ (the 1-3) ︰ (2-4) of Yang Hua ︰ Radix Flemingiae Philippinensis=(2-4);
(7) seal up for safekeeping again after blending 3 months above.
3. preparation method according to claim 2, is characterized in that: the weight part ratio that described each medicine fermentation liquid of step (6) is blent is: the Tai ︰ Kuan Jin Teng ︰ Shan Gui Hua ︰ Qi leaf Yi Zhi Hua ︰ that gallops along on horseback is Xiang Yang Hua ︰ Radix Flemingiae Philippinensis=3 ︰ 4 ︰ 4 ︰ 0.5 ︰ 2 ︰ 3.
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| CN201210287884.4A CN102784205B (en) | 2011-10-02 | 2012-08-14 | Externally-applied medicine for treating rheumatoid diseases and preparation method thereof |
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