CN102370860A - Externally applied medicine for treating rheumatoid arthritis and preparation method thereof - Google Patents
Externally applied medicine for treating rheumatoid arthritis and preparation method thereof Download PDFInfo
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- CN102370860A CN102370860A CN2011102921996A CN201110292199A CN102370860A CN 102370860 A CN102370860 A CN 102370860A CN 2011102921996 A CN2011102921996 A CN 2011102921996A CN 201110292199 A CN201110292199 A CN 201110292199A CN 102370860 A CN102370860 A CN 102370860A
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Abstract
The invention provides an externally applied medicine for treating rheumatoid arthritis and a preparation method thereof. The preparation method comprises the following steps: respectively soaking giantleaf ardisia rhizome, Kuanbianteng, Paramichelia baillonii, Paris polyphylla, sunflower and Philippine flemingia root in boiled rice vinegar; inoculating Acetobacter sp. GLYSM66 CCTCC NO:M2011238, and fermenting for different fermentation times; and blending the fermentation liquids to obtain the externally applied medicine for treating rheumatoid arthritis. The externally applied medicine for treating rheumatoid arthritis can relieve pain of patients with rheumatoid arthritis, and has the advantages of favorable treatment effect, low cost, simple preparation method and no toxic or side effect.
Description
Technical field
The present invention relates to application compress, specifically is rheumatoid application compress of a kind of treatment and preparation method thereof.
Background technology
Rheumatoid is claimed rheumatoid arthritis again, is main clinical manifestation with pathological changes outside chronic, symmetry, many synovial jointss inflammation and the joint, belongs to the autoimmune inflammatory diseases.Joint red and swollen heat pain and dysfunction are arranged in early days, and late period, stiff deformity in various degree can appear in the joint, and with bone and skeletal muscle atrophy, very easily disabled.At present, rheumatoid treatment has two kinds of doctor trained in Western medicine and the traditional Chinese medical science, and doctor trained in Western medicine mainly is a pain management, naturopathy, and closed treating etc., this all can only relief of symptoms, and side effect is big; The traditional Chinese medical science thinks that this disease is that meridian stasis stagnates institute extremely, treats this disease and should focus on the function of regulating the vital organs of the human body.Because the processing procedure of most of Chinese medicines is through boiling, extracting the method for the effective ingredient in the Chinese crude drug with decocting in water.But this method has following shortcoming: 1, owing to boil time, temperature, pressure and can not unify, the effective ingredient in the Chinese crude drug is difficult to be extracted out fully; 2, it is very high to boil in the process temperature, might destroy the effective ingredient of those heat sensitivitys; 3, be the intermediate of effective ingredient mostly through boiling the Chinese medicine ingredients that lixiviate goes out merely; Must transform through the intravital probiotic bacteria of people, finally become acting effective ingredient, get into blood; Because human body probiotic bacteria situation is different; Thereby produce the situation such as slow or ineffective that takes effect, therapeutic effect is unsatisfactory, and the exploitation low toxicity, the resisting rheumatoid disease new drug is the vital task of rheumatoid disease control efficiently.
Summary of the invention
To the deficiency of prior art, the present invention provide a kind of pain relieving fast, can channel recuperating, good effect, the rheumatoid application compress of treatment that have no side effect again.
Another object of the present invention provides the method for preparing of these application compress.
The technical scheme that realizes the object of the invention is:
The rheumatoid application compress of a kind of treatment; It is to be immersed in respectively in boiling refrigerative rice vinegar by Rhizoma Ardisiae Gigantifoliae, KUANBIANTENG, Radix Polygalae Wattersii, Rhizoma Paridis, Flos Galinsogae Parviflorae, Radix Flemingiae Philippinensis Chinese crude drug; Inoculate acetobacter, when different fermentations, behind the indirect fermentation, its fermentation liquid is blent processed again.
Described acetobacter is acetobacter (Acetobacter sp.) GLYSM66 CCTCC NO:M2011238; Be called for short acetobacter GLYSM66; Be that the inventor screens from common acetobacter; (be called for short: CCTCC), deposit number is CCTCC NO:M2011238 to be deposited in the typical Culture Center of Wuhan University China on July 5th, 2011.This acetobacter has the function of degraded several kinds of Chinese medicinal materials or composite Chinese crude drug.
The method for preparing of application compress of the present invention comprises the steps:
(1) Chinese crude drug Rhizoma Ardisiae Gigantifoliae, KUANBIANTENG, Radix Polygalae Wattersii, Rhizoma Paridis, Flos Galinsogae Parviflorae, Radix Flemingiae Philippinensis pulverize separately are crossed 100 orders, and autoclaving 30 minutes, subsequent use;
(2) rice vinegar is boiled with warm fire, naturally cool to room temperature, subsequent use;
(3) six kinds of Chinese crude drugs step (1) being handled are immersed in respectively in the rice vinegar after step (2) sterilization, and solid-liquid ratio is 1 ︰ (8-10);
(4) with acetobacter GLYSM66 with LB culture medium amplification culture after, be inoculated into respectively in each medical material soak, its inoculum concentration is: medical material soaks the ︰ 1 of the volume ratio of Ye ︰ strain=(20-50);
(5) ferment at normal temperatures, fermentation time is respectively: Rhizoma Ardisiae Gigantifoliae 145-155 days, KUANBIANTENG 115-130 days, Radix Polygalae Wattersii 85-100 days, Rhizoma Paridis 175-185 days, Flos Galinsogae Parviflorae 145-155 days, Radix Flemingiae Philippinensis 115-130 days;
(6) blend: get above-mentioned each medicine fermentation liquid and blend by following weight part ratio: the colored ︰ of osmanthus, tire ︰ broadside rattan ︰ mountain flower ︰ seven leaves that gallops along on horseback is to ︰ (3-5) ︰ (3-5) ︰ (0.4-0.6) ︰ (1-3) ︰ (2-4) of the colored ︰ Radix Flemingiae Philippinensis of sun=(2-4);
(7) seal up for safekeeping again after blending more than 3 months.
The method for using of this medicine has two kinds, can use wherein a kind of, also can two kinds of combinations:
First method is:
(1) take a shower earlier, patient's pore is fully opened after, with medicinal liquid fumigation 30 minutes to 1 hour;
(2) put on the clothes that soaked with medicinal liquid, the parcel health;
(3) toast whole body with infrared ray;
(4) carry out channels and network vessels regulation.
10 days is a course of treatment.
Second method is: external application, suction medicinal liquid with absorbent paper, and apply at the rheumatoid position, the external preservative film encases, and fixes with adhesive tape, and applied 6 hours every day at least, and 7 days is a course of treatment.
The external application of the pharmacological effects of various herbs, according to "Chinese Dictionary" records: Sunflower with Liver eyesight; polyphylla with detoxification, blood carbuncle, carminative dehumidification sore; Flemingia has eradicates rheumatism, pain , carminative blood function; cursory tires have chills, swelling and pain; baillonii with Shujin scattered blood, blood detoxification, wide-brimmed rattan Qufengchushi, Shujin, swelling and pain in the function of these medicines by Acetobacter invention fermentation, decomposition and transformation of the medicines, the active ingredient is fully released, enhanced potency, and acetic acid bacteria and the presence of strong acids, enhances the oxidation resistance of the active ingredient, with more blood circulation , Shujin, carminative dehumidification, gas line anti-inflammatory analgesic function.Clinical use result shows that this medicine treatment rheumatoid effect is remarkable, and medical expense is low, and method for preparing is simple, has no side effect again.
Clinical this medicine of usefulness treatment rheumatoid 15 examples, significant curative effect 13 examples wherein, effective 2 examples, and skin had no side effect.
The specific embodiment
Below through embodiment the present invention is described.
Used acetobacter GLYSM66 obtains through the screening technique that comprises the steps in the present embodiment:
(1) gets Flos Galinsogae Parviflorae, Semen Fagopyri Esculenti root, very heavy group, YIDUOYUN or five kinds of Chinese crude drugs of Rhizoma Paridis, pulverize, cross 100 mesh sieves, autoclaving 30 minutes respectively afterwards, five kinds of Chinese crude drug mixed in equal amounts or deposit subsequent use separately;
(2) use the soaking in rice vinegar medical material, medical material compares public jin ︰ liter with the mass and size of rice vinegar)=1 ︰ (5-10), soaking temperature 20-35 ℃, the soaking fermentation different time;
(3) get the fermentation liquid of different fermentations time; Measure effective ingredient--total flavones and total saponins; Simultaneously through 1000 power microscope microscopies; Total flavones and the total saponin content in the sample of five kinds of Chinese crude drug mixed in equal amounts and the five kinds of single samples of Chinese crude drug relatively, the result finds: biased sample is total flavones and total saponin content height not only, and only has single microorganism in the fermentation liquid; Microbe species in latter's liquid is many.Separation screening is carried out in microorganism to biased sample, and successive transfer culture obtains the acetobacter of the several kinds of Chinese medicinal materials of degrading of the present invention.
The used LB culture medium of the amplification culture of acetobacter described in the present embodiment is by sodium chloride 10g, and peptone 10g, yeast extract 5g add water and be settled to 1L and process.
Embodiment 1
A kind of method for preparing of treating rheumatoid application compress comprises the steps:
(1) Chinese crude drug Rhizoma Ardisiae Gigantifoliae, KUANBIANTENG, Radix Polygalae Wattersii, Rhizoma Paridis, Flos Galinsogae Parviflorae, Radix Flemingiae Philippinensis pulverize separately are crossed 100 orders, and autoclaving 30 minutes, subsequent use;
(2) rice vinegar is boiled with warm fire, naturally cool to room temperature, subsequent use;
(3) six kinds of Chinese crude drugs step (1) being handled are immersed in respectively in the rice vinegar after step (2) sterilization, and solid-liquid ratio is 1 ︰ (8-10);
(4) with acetobacter GLYSM66 with LB culture medium amplification culture after, be inoculated into respectively in each medical material soak, its inoculum concentration is: medical material soaks the ︰ 1 of the volume ratio of Ye ︰ strain=(20-50);
(5) ferment at normal temperatures, fermentation time is respectively: Rhizoma Ardisiae Gigantifoliae 150 days, KUANBIANTENG 120 days, Radix Polygalae Wattersii 90 days, Rhizoma Paridis 180 days, Flos Galinsogae Parviflorae 150 days, Radix Flemingiae Philippinensis 120 days;
(6) blend: the weight part ratio (kilogram) that each medicine fermentation liquid is blent is: the Tai ︰ broadside Teng ︰ Shan Gui Hua ︰ Qi leaf Yi Zhi Hua ︰ that gallops along on horseback is Xiang Yang Hua ︰ Radix Flemingiae Philippinensis=3 ︰, 4 ︰, 4 ︰, 0.5 ︰, 2 ︰ 3;
(7) seal up for safekeeping again after blending and promptly processed the compound Chinese medicine preparation in 90 days.
Embodiment 2
Method step according to embodiment 1 carries out; Different is: the weight part ratio (kilogram) that said each the medicine fermentation liquid of step (6) is blent is: the Tai ︰ broadside Teng ︰ Shan Gui Hua ︰ Qi leaf Yi Zhi Hua ︰ that gallops along on horseback sealed up for safekeeping after blending 110 days Xiang Yang Hua ︰ Radix Flemingiae Philippinensis=2.5 ︰, 3.5 ︰ .3.5 ︰, 0.4 ︰, 1.5 ︰ 2.5 again.
Embodiment 3
Use this compound Chinese medicine preparation for treating hand rheumatoid effect instance:
1, patient: Lee, man, 62 years old; Guilin people from city suffered from rheumatoid more than 10 year, symptom before the treatment: the both hands elbow joint is crooked; Can not stretch normal amplitude, the appearance pain points that whole body can be indefinite, the people is thin like bavin; After with this medicine treatment conditioning, the pain spot and the pain degree of whole body reduce gradually, recovery from illness after 60 days.
2, patient: Lee, the woman, 33 years old, Guilin city people from Pingyue County was engaged in agriculture; Before the treatment, both hands are from all enlargements of joints of hand to articulations digitorum manus, ankle joint to the instep enlargement of both feet, difficult round the clock the dormancy; Stating according to me is that to live is no better than to die, skinny and wizened, the collapse of giving sb. a hard time, and the also frequent indefinite pain spot of whole body is through treatment; After the drug of topical application 6 hours, pain reduces gradually, and continuous use is nursed one's health recovery from illness in 45 days.
Claims (3)
1. treat rheumatoid application compress for one kind; It is characterized in that: it is to be immersed in respectively in the rice vinegar of boiling by Rhizoma Ardisiae Gigantifoliae, KUANBIANTENG, Radix Polygalae Wattersii, Rhizoma Paridis, Flos Galinsogae Parviflorae, Radix Flemingiae Philippinensis Chinese crude drug; Inoculate acetobacter; When different fermentations, behind the indirect fermentation, its fermentation liquid is blent processed again, wherein:
Described acetobacter is acetobacter (Acetobacter sp.) GLYSM66 CCTCC NO:M2011238;
The solid-liquid ratio of said each medical material and rice vinegar is 1 ︰ (8-10);
The weight part ratio that said each fermentation liquid is blent is: the Tai ︰ broadside Teng ︰ Shan Gui Hua ︰ Qi leaf Yi Zhi Hua ︰ that gallops along on horseback is Xiang ︰ (3-5) ︰ (3-5) ︰ (0.4-0.6) ︰ (the 1-3) ︰ (2-4) of Yang Hua ︰ Radix Flemingiae Philippinensis=(2-4).
2. the method for preparing of the rheumatoid application compress of treatment is characterized in that: comprise the steps:
(1) Chinese crude drug Rhizoma Ardisiae Gigantifoliae, KUANBIANTENG, Radix Polygalae Wattersii, Rhizoma Paridis, Flos Galinsogae Parviflorae, Radix Flemingiae Philippinensis pulverize separately are crossed 100 orders, and autoclaving 30 minutes, subsequent use;
(2) rice vinegar is boiled with warm fire, naturally cool to room temperature, subsequent use;
(3) five kinds of Chinese crude drugs step (1) being handled well are immersed in respectively in the rice vinegar after step (2) sterilization, and solid-liquid ratio is 1 ︰ (8-10);
(4) with acetobacter GLYSM66 with LB culture medium amplification culture after, be inoculated into respectively in each medical material soak, its inoculum concentration is: medical material soaks the ︰ 1 of the volume ratio of Ye ︰ strain=(20-50);
(5) ferment at normal temperatures, fermentation time is respectively: Rhizoma Ardisiae Gigantifoliae 145-155 days, KUANBIANTENG 115-130 days, Radix Polygalae Wattersii 85-100 days, Rhizoma Paridis 175-185 days, Flos Galinsogae Parviflorae 145-155 days, Radix Flemingiae Philippinensis 115-130 days;
(6) blend: get above-mentioned each medicine fermentation liquid and blend, its weight part ratio is: the Tai ︰ broadside Teng ︰ Shan Gui Hua ︰ Qi leaf Yi Zhi Hua ︰ that gallops along on horseback is Xiang ︰ (3-5) ︰ (3-5) ︰ (0.4-0.6) ︰ (the 1-3) ︰ (2-4) of Yang Hua ︰ Radix Flemingiae Philippinensis=(2-4);
(7) seal up for safekeeping again after blending more than 3 months.
3. method for preparing according to claim 2 is characterized in that: the weight part ratio that said each the medicine fermentation liquid of step (6) is blent is: the Tai ︰ broadside Teng ︰ Shan Gui Hua ︰ Qi leaf Yi Zhi Hua ︰ that gallops along on horseback is Xiang Yang Hua ︰ Radix Flemingiae Philippinensis=3 ︰, 4 ︰, 4 ︰, 0.5 ︰, 2 ︰ 3.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011102921996A CN102370860A (en) | 2011-10-02 | 2011-10-02 | Externally applied medicine for treating rheumatoid arthritis and preparation method thereof |
| CN201210287884.4A CN102784205B (en) | 2011-10-02 | 2012-08-14 | Externally-applied medicine for treating rheumatoid diseases and preparation method thereof |
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| CN2011102921996A CN102370860A (en) | 2011-10-02 | 2011-10-02 | Externally applied medicine for treating rheumatoid arthritis and preparation method thereof |
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| CN201210287884.4A Active CN102784205B (en) | 2011-10-02 | 2012-08-14 | Externally-applied medicine for treating rheumatoid diseases and preparation method thereof |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| TWI556841B (en) * | 2012-06-05 | 2016-11-11 | Lai-Cheng Wang | A method of adsorbing a root extract to a fiber |
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| CN100540020C (en) * | 2005-09-11 | 2009-09-16 | 王浩贵 | A kind of Flos Carthami medicine for external use |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| TWI556841B (en) * | 2012-06-05 | 2016-11-11 | Lai-Cheng Wang | A method of adsorbing a root extract to a fiber |
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| CN102784205B (en) | 2014-03-19 |
| CN102784205A (en) | 2012-11-21 |
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Application publication date: 20120314 |