CN102771874A - Natural food preservative and preparation method and applications thereof - Google Patents
Natural food preservative and preparation method and applications thereof Download PDFInfo
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- CN102771874A CN102771874A CN2012102466346A CN201210246634A CN102771874A CN 102771874 A CN102771874 A CN 102771874A CN 2012102466346 A CN2012102466346 A CN 2012102466346A CN 201210246634 A CN201210246634 A CN 201210246634A CN 102771874 A CN102771874 A CN 102771874A
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Abstract
The invention provides a natural food preservative which comprises the following ingredients in parts by weight: 3-5 parts of common lophatherum herb, 1-3 parts of honeysuckle, 1-3 parts of purple perilla and 1-3 parts of rhizome imperatae. The invention further provides a preparation method and applications of the natural food preservative. The natural food preservative is prepared by mixing the common lophatherum herb, the honeysuckle and other medicinal and edible natural substances, has the characteristics of being good in antibiosis property, safe and non-toxic, wide in range of application and the like. The natural food preservative can be degraded into substances capable of being utilized by human body in digestive tract of the human body, thus greatly avoiding cancerigenic, teratogenic, mutational and the like damages caused by residual of chemical preservatives in the body.
Description
Technical field
The invention belongs to the food antiseptic field, relate to a kind of antiseptics for natural food.
Background technology
" bread is the staff of life, and food is with An Weixian ", food-safety problem has obtained the great attention of consumer and country.A large amount of both at home and abroad at present food preservatives that use are main with the chemical synthesis anticorrisive agent, and chemical preservative is low with its production cost, and advantages such as production technology maturation are in occupation of the leading position in anticorrisive agent market.But in recent years, the expert constantly queries to the security of chemical preservative both at home and abroad.Along with the raising of The development in society and economy and living standards of the people, people's concern food-safety problem is all the more constantly pursued more natural, safe, healthy food.Country is also in the supervision and management of strengthening year by year food security.A kind of nontoxic natural antiseptic agent of market in urgent need.
Summary of the invention
Goal of the invention: the purpose of this invention is to provide a kind of nontoxic antiseptics for natural food and preparation method thereof.
Technical scheme: antiseptics for natural food provided by the invention, process by the component of following parts by weight: lophatherum gracile 3-5 part, honeysuckle 1-3 part, purple perilla 1-3 part and cogongrass rhizome 1-3 part.
A kind of preferred as antiseptics for natural food of the present invention processed by the component of following parts by weight: 4 parts of lophatherum graciles, 2 parts of honeysuckles, 2 parts of purple perillas, 2 parts of cogongrass rhizomes.
The present invention also provides a kind of preparation method of above-mentioned antiseptics for natural food, may further comprise the steps:
(1) lophatherum gracile, honeysuckle, perilla leaf and cogongrass rhizome are mixed, boiling is filtered, and being concentrated into crude drug concentration is 1~1.3g/ml, gets decocting and concentrating liquid;
(2) in decocting and concentrating liquid, add ethanol, make concentration of alcohol reach 80-85%, leave standstill, filter, must filtrate;
(3) filtrating activated carbon decolorizing, ultrafiltration, drying promptly gets.
Wherein, in the step (1), each amount of water be each component gross mass 8-10 doubly, decocting time is 1-3h, the decoction number of times is 1-3 time; Through the control decocting condition, active ingredient that can the high efficiency extraction raw material makes preservative effectiveness better.
In the step (2), time of repose is 12-36h, through the control time of repose, can effectively separate impurity in the decocting and concentrating liquid.
In the step (3), the amount ratio of said active carbon and filtrating is (0.3-0.6) g:100mL; Bleaching temperature is 35-45 ℃, and bleaching time is 20-40min.
The present invention also provides the above-mentioned application of antiseptics for natural food in food antiseptic.This antiseptics for natural food all has significant inhibitory effect to strain growths such as staphylococcus aureus common in the food, Escherichia coli, bacillus subtilis and Candida albicans, can be effectively anticorrosion.
Beneficial effect: antiseptics for natural food provided by the invention is formed by the pure natural substance compatibility of integration of drinking and medicinal herbs such as lophatherum gracile, honeysuckle, has that antibiotic property is good, a characteristic such as safety non-toxic, sphere of action are wide.This natural antiseptic agent can be degraded to the utilizable composition of human body in human body alimentary canal, harm such as carcinogenic, the teratogenesis of greatly having avoided causing because of chemical preservative is residual in vivo, sudden change.
This antiseptics for natural food not only can effectively suppress microbial growth such as bacterium, mould in the food, prolongs effective period of food quality, plays the corrosion-resistant effect, also have simultaneously clearing heat-fire, promoting the circulation of qi wide in, the booster action curative effect of clearing damp and promoting diuresis.
The specific embodiment
According to following embodiment, can understand the present invention better.Yet, those skilled in the art will readily understand that the described concrete material proportion of embodiment, process conditions and result thereof only are used to explain the present invention, and the present invention that should also can not limit in claims to be described in detail.
Embodiment 1:
Antiseptics for natural food comprises following components in weight percentage: 3 parts of lophatherum graciles, 3 parts of honeysuckles, 1 part of purple perilla, 3 parts of cogongrass rhizomes.
Its preparation method:
Get lophatherum gracile 3kg, honeysuckle 3kg, purple perilla 1kg and cogongrass rhizome 3kg four traditional Chinese medicine material, mixing boiled 3 hours with the decocting of 8 times of amounts, filtered, and the dregs of a decoction were decocted 3 hours once more, filtered, and merged decoction liquor, and being concentrated into crude drug concentration is 1g/ml, decocting and concentrating liquid;
Add ethanol in the decocting and concentrating liquid, make its concentration of alcohol reach 80%, left standstill 24 hours, filtering and impurity removing must be filtrated.
Add active carbon in the filtrating, wherein the amount ratio of active carbon and filtrating is 0.5g:100ml,, stir, with feed liquid heat temperature raising to 40 ℃, insulation decolouring 0.5h; Centrifugal; To decolour the centrifugal clear solution that obtains through ultrafiltration membrance filter, and the pressure when wherein filtering is controlled to be 0.05~0.15MPa, and the specified aperture of milipore filter may be selected to be 0.001~0.02m.The employing milipore filter separates, and gets the active liquid component, with active liquid component spray-drying, makes liquid mist change into tiny droplet, exchanges with thermal current, and then is dried to powder, promptly gets.
Embodiment 2:
Antiseptics for natural food comprises following components in weight percentage: 4 parts of lophatherum graciles, 2 parts of honeysuckles, 2 parts of purple perillas, 2 parts of cogongrass rhizomes.
Its preparation method:
Get lophatherum gracile 4kg, honeysuckle 2kg, purple perilla 2kg and cogongrass rhizome 2kg four traditional Chinese medicine material, mixing boiled 2 hours with the decocting of 9 times of amounts, filtered, and the dregs of a decoction were decocted 2 hours once more, filtered, and merged decoction liquor, and being concentrated into crude drug concentration is 1.2g/ml, decocting and concentrating liquid;
Add ethanol in the decocting and concentrating liquid, make its concentration of alcohol reach 85%, left standstill 24 hours, filtering and impurity removing must be filtrated.
Add active carbon in the filtrating, wherein the amount ratio of active carbon and filtrating is 0.3g:100ml, stir, and with feed liquid heat temperature raising to 45 ℃, insulation decolouring 40min; Centrifugal; To decolour the centrifugal clear solution that obtains through ultrafiltration membrance filter, and the pressure when wherein filtering is controlled to be 0.05~0.15MPa, and the specified aperture of milipore filter may be selected to be 0.001~0.02m.The employing milipore filter separates, and gets the active liquid component, with active liquid component spray-drying, makes liquid mist change into tiny droplet, exchanges with thermal current, and then is dried to powder, promptly gets.
Embodiment 3:
Antiseptics for natural food comprises following components in weight percentage: 5 parts of lophatherum graciles, 1 part of honeysuckle, 3 parts of purple perillas, 1 part of cogongrass rhizome.
Its preparation method:
Get lophatherum gracile 5kg, honeysuckle 1kg, purple perilla 3kg and cogongrass rhizome 1kg four traditional Chinese medicine material, mixing boiled 1 hour with the decocting of 10 times of amounts, filtered, and the dregs of a decoction were decocted 1 hour once more, filtered, and merged decoction liquor, and being concentrated into crude drug concentration is 1.3g/ml, decocting and concentrating liquid;
Add ethanol in the decocting and concentrating liquid, make its concentration of alcohol reach 85%, left standstill 24 hours, filtering and impurity removing must be filtrated.
Add active carbon in the filtrating, wherein the amount ratio of active carbon and filtrating is 0.6g:100ml, stir, and with feed liquid heat temperature raising to 35 ℃, insulation decolouring 20min; Centrifugal; To decolour the centrifugal clear solution that obtains through ultrafiltration membrance filter, and the pressure when wherein filtering is controlled to be 0.05~0.15MPa, and the specified aperture of milipore filter may be selected to be 0.001~0.02m.The employing milipore filter separates, and gets the active liquid component, with active liquid component spray-drying, makes liquid mist change into tiny droplet, exchanges with thermal current, and then is dried to powder, promptly gets.
Embodiment 4:
Antiseptics for natural food comprises following components in weight percentage: 3 parts of lophatherum graciles, 1 part of honeysuckle, 1 part of purple perilla, 1 part of cogongrass rhizome.
Its preparation method:
Get lophatherum gracile 3kg, honeysuckle 1kg, purple perilla 1kg and cogongrass rhizome 1kg four traditional Chinese medicine material, mixing boiled 2 hours with the decocting of 9 times of amounts; Filter, the dregs of a decoction continue to boil each 2h 2 times with the decocting of 9 times of amounts, merge decoction liquor; Being concentrated into crude drug concentration is 1.2g/ml, gets decocting and concentrating liquid;
Add ethanol in the decocting and concentrating liquid, make its concentration of alcohol reach 80%, left standstill 36 hours, filtering and impurity removing must be filtrated.
Add active carbon in the filtrating, wherein the amount ratio of active carbon and filtrating is 0.5g:100ml,, stir, with feed liquid heat temperature raising to 40 ℃, insulation decolouring 0.5h; Centrifugal; To decolour the centrifugal clear solution that obtains through ultrafiltration membrance filter, and the pressure when wherein filtering is controlled to be 0.05~0.15MPa, and the specified aperture of milipore filter may be selected to be 0.001~0.02m.The employing milipore filter separates, and gets the active liquid component, with active liquid component spray-drying, makes liquid mist change into tiny droplet, exchanges with thermal current, and then is dried to powder, promptly gets.
Embodiment 5:
Antiseptics for natural food comprises following components in weight percentage: 5 parts of lophatherum graciles, 3 parts of honeysuckles, 3 parts of purple perillas, 3 parts of cogongrass rhizomes.
Its preparation method:
Get lophatherum gracile 5kg, honeysuckle 3kg, purple perilla 3kg and cogongrass rhizome 3kg four traditional Chinese medicine material, mixing boiled 3 hours with the decocting of 10 times of amounts, filtered, and being concentrated into crude drug concentration is 1.1g/ml, decocting and concentrating liquid;
Add ethanol in the decocting and concentrating liquid, make its concentration of alcohol reach 85%, left standstill 12 hours, filtering and impurity removing must be filtrated;
Add active carbon in the filtrating, wherein the amount ratio of active carbon and filtrating is 0.4g:100ml,, stir, with feed liquid heat temperature raising to 40 ℃, insulation decolouring 0.5h; Centrifugal; To decolour the centrifugal clear solution that obtains through ultrafiltration membrance filter, and the pressure when wherein filtering is controlled to be 0.05~0.15MPa, and the specified aperture of milipore filter may be selected to be 0.001~0.02m.The employing milipore filter separates, and gets the active liquid component, with active liquid component spray-drying, makes liquid mist change into tiny droplet, exchanges with thermal current, and then is dried to powder, promptly gets.
Embodiment 6
Below the antiseptics for natural food fungistatic effect experiment of embodiment 1:
The prescription dilution:
Get the antiseptics for natural food 5g of embodiment 1, add 10ml distilled water, prepare to such an extent that the prescription diluent concentration is 0.5g/ml.
Culture medium:
Get nutrient broth medium 5g (it consists of: peptone 2.8g, powdered beef 0.8g, sodium chloride 1.4g, pH value 7.2 ± 0.2), add 200ml distilled water, mixing, sterilization (sterilising conditions is: 102.9kPa, and 121 ℃, 20min).
Get improvement Martin culture medium 2.85g (it consists of: peptone 0.5g, dipotassium hydrogen phosphate 0.1g, magnesium sulfate 0.05g, dusty yeast 0.2g, glucose 2g), add distilled water 100ml, mixing, sterilization (sterilising conditions is: 102.9kPa, and 121 ℃, 20min).
Bacteria Culture:
Get staphylococcus aureus (ATCC25923), Escherichia coli (ATCC441490) and bacillus subtilis (CMCC63501) bacterial strain, be inoculated in respectively in the nutrient broth medium, cultivate 18h for 37 ℃, get each strain culture.Get each strain culture, do the 1:1000 dilution with nutrient broth and be used for experiment, must dilute bacterial strain liquid.
Fungal culture:
Extracting waste candida albicans (CMCC98001) bacterial strain a little, be inoculated in the improvement Martin culture medium, cultivate 24h for 35 ℃, the Candida albicans culture.Get the Candida albicans culture that 24h cultivates, do the 1:1000 dilution with improvement Martin culture medium and be used for experiment, must dilute bacterium liquid.
Doubling dilution is measured antibacterial activity:
Staphylococcus aureus group A group: get 9 in sterilization test tube, the 1st pipe adds nutrient broth medium 1ml, and second pipe adds 1.6ml, and all the other every pipes add 1ml.The 1st pipe does not add the prescription dilution as contrast; Get prescription dilution 0.4ml and add in the 2nd pipe, get 1ml to the 3 pipes behind the mixing, dilution successively; Discard 1ml in the 8th pipe.Every pipe adds dilution bacterium liquid 0.1ml, cultivates 24h for 37 ℃.
Escherichia coli group B group, method is organized with A.
Bacillus subtilis group C group, method is organized with A.
Candida albicans group D group: get 9 in sterilization test tube, the 1st pipe adds improvement Martin culture medium 1ml, and the 2nd pipe adds 1.6m, all the other every pipe 1ml.The 1st pipe does not add the prescription dilution as contrast; Get prescription dilution 0.4ml and add in the 2nd pipe, get 1ml to the 3 pipes behind the mixing, dilution successively; Discard 1ml in the 8th pipe.Every pipe adds dilution bacterium liquid 0.1ml, cultivates 24h for 35 ℃.
Observe the conk situation.Muddy like test tube, promptly represent bacterial growth, supply examination prescription dilution not have bacteriostasis, represent then that as test tube is limpid conk is suppressed, the soup that it can suppress the greatest dilution of conk is the minimal inhibitory concentration of this prescription.The result sees table 1.
The antibacterial effect of the antiseptics for natural food of table 1 embodiment 1
Experimental result shows that this antiseptics for natural food all has significant inhibitory effect to strain growths such as staphylococcus aureus common in the food, Escherichia coli, bacillus subtilis and Candida albicans, can be effectively anticorrosion.This antiseptics for natural food concentration promptly produced the obvious suppression effect to staphylococcus aureus and Escherichia coli at 0.625% o'clock, at 1.25% o'clock bacillus subtilis, Candida albicans was produced the obvious suppression effect.
Claims (7)
1. antiseptics for natural food, it is characterized in that: the component by following parts by weight is processed: lophatherum gracile 3-5 part, honeysuckle 1-3 part, purple perilla 1-3 part and cogongrass rhizome 1-3 part.
2. a kind of antiseptics for natural food according to claim 1 is characterized in that: the component by following parts by weight is processed: 4 parts of lophatherum graciles, 2 parts of honeysuckles, 2 parts of purple perillas, 2 parts of cogongrass rhizomes.
3. the preparation method of each described antiseptics for natural food of claim 1 to 2 is characterized in that: may further comprise the steps:
(1) lophatherum gracile, honeysuckle, perilla leaf and cogongrass rhizome are mixed, boiling is filtered, and being concentrated into crude drug concentration is 1~1.3g/ml, gets decoction liquor;
(2) in decocting and concentrating liquid, add ethanol, make concentration of alcohol reach 80-85%, leave standstill, filter, must filtrate;
(3) filtrating is used activated carbon decolorizing, ultrafiltration, and drying promptly gets.
4. the preparation method of a kind of antiseptics for natural food according to claim 3 is characterized in that: in the step (1), amount of water be each component gross mass 8-10 doubly, decocting time is 1-3h, decocting number of times is 1-3 time.
5. the preparation method of a kind of antiseptics for natural food according to claim 3, it is characterized in that: in the step (2), time of repose is 12-36h.
6. the preparation method of a kind of antiseptics for natural food according to claim 3, it is characterized in that: in the step (3), the amount ratio of said active carbon and filtrating is (0.3-0.6) g:100mL; Bleaching temperature is 35-45 ℃, and bleaching time is 20-40min.
7. the application of the described antiseptics for natural food of claim 1 in food antiseptic.
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CN103689133A (en) * | 2013-12-13 | 2014-04-02 | 广西科技大学 | Green and antibacterial supercritical CO2 boxthorn seed oil extraction additive |
CN104938544A (en) * | 2015-06-24 | 2015-09-30 | 陈琳仁 | Method for preparing natural preservative and application thereof |
CN105076246A (en) * | 2015-08-28 | 2015-11-25 | 无限极(中国)有限公司 | Antibacterial traditional Chinese medicine composition, preparing method of antibacterial traditional Chinese medicine composition and application of antibacterial traditional Chinese medicine composition |
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CN103689133A (en) * | 2013-12-13 | 2014-04-02 | 广西科技大学 | Green and antibacterial supercritical CO2 boxthorn seed oil extraction additive |
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CN105076246A (en) * | 2015-08-28 | 2015-11-25 | 无限极(中国)有限公司 | Antibacterial traditional Chinese medicine composition, preparing method of antibacterial traditional Chinese medicine composition and application of antibacterial traditional Chinese medicine composition |
CN107319085A (en) * | 2017-07-11 | 2017-11-07 | 广东百盛食品有限公司 | A kind of semisolid candy and its processing technology |
CN107518245A (en) * | 2017-09-08 | 2017-12-29 | 郭迎庆 | A kind of preparation method of food preservative |
CN107788324A (en) * | 2017-09-22 | 2018-03-13 | 安徽丹研食品有限公司 | A kind of highly efficiency compositional food preservative and preparation method thereof |
CN109805241A (en) * | 2017-11-20 | 2019-05-28 | 柳州市皇之品贸易有限公司 | One kind preservative containing Chinese herbal medicine food and preparation method thereof |
CN108013129A (en) * | 2017-12-16 | 2018-05-11 | 柳州市晟铂贸易有限公司 | A kind of rice natural antisepsis fresh-keeping agent and preparation method thereof |
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Application publication date: 20121114 |