CN104938544A - Method for preparing natural preservative and application thereof - Google Patents
Method for preparing natural preservative and application thereof Download PDFInfo
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Abstract
The invention discloses a method for preparing a natural preservative and application thereof. The natural preservative is composed of, by mass percentage, 40-58% of bamboo leaf extract, 20-30% of tea extract, 20-30% of licorice extract, 0.1-1% of lemon essential oil and 0.1-1% of cajeput essential oil; and the total of the components is 100%. The method comprises mixing the bamboo leaf extract, the tea extract and the licorice extract, adding the lemon essential oil and the cajeput essential oil, and fully evenly mixing the mixture so that the essential oil is fully absorbed and the natural preservative is obtained. The natural preservative can be applied to foods, medicines, chemical engineering, hygienic products, articles for daily use and cosmetics and is concretely added according to 0.05-3% of the product mass. The preservative improves antibacterial effect, expands an antibacterial spectrum and further has effects of virus resistance, cold prevention and throat heat clearing; and the preservative not only serves as a food additive, but also has a certain pharmacological health care effect.
Description
Technical field
The invention belongs to preservative technology field, relate to a kind of natural antiseptic agent preparation method and application thereof.
Background technology
Food preservative main at present has chemical preservative and natural antiseptic agent.Chemical preservative low price, output are large, so be still main food preservative at present.But chemical preservative has safety problem in various degree, the preservative of partial chemical synthesis can accumulate in vivo and cause poisoning, or causes food origin disease, carcinogenic.More and more pursue the trend of Nantural non-toxic food consumer under, utilize metabolite of animals and plants or microorganism etc. as raw material, the antiseptics for natural food utilizing modern biotechnology means to produce more and more is subject to people's attention, natural antiseptic agent has the incomparable advantage of the synthetic preservatives such as antibiotic property is strong, safety non-toxic, good water solubility, Heat stability is good, sphere of action are wide, therefore develops efficient, safe, stable natural antiseptic agent one of focus becoming food scientific research.
Summary of the invention
Easily cause poisoning for solving existing chemical preservative, and the problems such as existing natural antiseptic agent poor effect, the invention provides a kind of natural antiseptic agent, preparation method and application thereof.
The present invention is realized by following technical proposal: a kind of natural antiseptic agent, be made up of the component of following masses percentage: bamboo extractive 40 ~ 58%, tea extract 20 ~ 30%, licorice 20 ~ 30%, lemon 0.1 ~ 1%, Melaleuca oil 0.1 ~ 1%, the summation of above each component is 100%.
Described bamboo extractive obtains through the following step:
1. by dry for the leaf of bamboo, be crushed to 20 ~ 40 orders, then count 1:10 ~ 50 with distilled water with solid-to-liquid ratio kg/L and carry out immersion 20 ~ 30min, obtain leaf of bamboo soak; Add the biology enzyme of leaf of bamboo soak cumulative volume 0.1 ~ 0.5%, then use citric acid adjust pH to 4 ~ 5, then at 50 ~ 80 DEG C, heat 2 ~ 3h, refilter and obtain filtrate and filter residue;
2. gained filter residue repeats step operation 1. 3 ~ 5 times, and merging filtrate, is concentrated into paste by filtrate, then dries at 60 ~ 80 DEG C, namely obtains bamboo extractive.
Described biology enzyme is cellulase and neutral proteinase, and cellulase and neutral proteinase are that arbitrary proportion adds.
Described tea extract obtains through the following step:
Tea leaf powder is broken to 20 ~ 40 orders, count 1:10 ~ 50 with distilled water with solid-to-liquid ratio kg/L again and carry out immersion 20 ~ 30min, then decoct 2h and filter, repeat to soak and decoct operation 3 ~ 5 times, merging filtrate, by filtrate through being evaporated to paste, then drying at 60 ~ 80 DEG C, namely obtaining tea extract.
Described licorice obtains through the following step:
By Radix Glycyrrhizae drying, be crushed to 20 ~ 40 orders, count 1:10 ~ 50 with distilled water with solid-to-liquid ratio kg/L again and carry out immersion 20 ~ 30min, then decoct 2h and filter, repeat to soak and decoct operation 3 ~ 5 times, merging filtrate, by filtrate through being evaporated to paste, then drying at 60 ~ 80 DEG C, namely obtaining licorice.
Described lemon obtains through the following step:
The distilled water adding lemon tablet weight 10 ~ 50 times in the lemon tablet dried in the shade carries out immersion 20 ~ 30min, obtain lemon tablet soak, add the cellulase of lemon tablet soak cumulative volume 0.5 ~ 1% again, and at 40 ~ 50 DEG C constant temperature 3 ~ 5h, then steam distillation is carried out, collect distillate by condenser, distillate cools layering at being placed in 3 ~ 4 DEG C, and upper strata is lemon.
Described Melaleuca oil obtains through the following step:
The distilled water adding cajeputtree weight 10 ~ 50 times in the cajeputtree dried in the shade carries out immersion 20 ~ 30min, obtain cajeputtree soak, add the cellulase of cajeputtree soak cumulative volume 0.5 ~ 1% again, and at 40 ~ 50 DEG C constant temperature 3 ~ 5h, then steam distillation is carried out, collect distillate by condenser, distillate cools layering at being placed in 3 ~ 4 DEG C, and upper strata is Melaleuca oil.
Described cellulase is beta-glucosidase, cellobiose and other low molecule cellodextrins can be decomposed into glucose.
Another object of the present invention is to the preparation method that a kind of natural antiseptic agent is provided, through following each step:
(1) get the raw materials ready by the component of following mass percent: bamboo extractive 40 ~ 58%, tea extract 20 ~ 30%, licorice 20 ~ 30%, lemon 0.1 ~ 1%, Melaleuca oil 0.1 ~ 1%, the summation of above each component is 100%;
(2) bamboo extractive, tea extract and licorice in step (1) are mixed, then add lemon and Melaleuca oil, fully mixing absorbs completely to essential oil, namely obtains natural antiseptic agent.
The application of described natural antiseptic agent in food, medicine, chemical industry, amenities, commodity, cosmetics, as food such as fruit juice, jam, fermented beverage, dairy products, cakes; Toothpaste, shampoo, bath foam, hand cleanser.
During the application of this natural antiseptic agent, be add by 0.05 ~ 3% of product quality.Wherein, the addition in food and drink is 1 ~ 2% of food and drink quality; Addition in daily chemical products is 0.05 ~ 1%; Addition in cosmetics is 2 ~ 3%.
The main source of bamboo extractive is the leaf of gramineous bamboo department linoleic plant, and its main antipathogenic composition is flavonoid glycoside and aglycon thereof, and it goes back Wheat Protein, and flavonoid glycoside content is higher, and antibacterial antiseptic power is stronger.The natural additive for foodstuff of prepared by the present invention have antibacterial action has good antibacterial action to leading septicogenic soil thermoduric bacteria, can stop the growth of bacterium, also effective to yeast, mould.Surimi product, meat products, grease article, rice and Noodles etc. can be widely used in.The antibacterial material that Japan extracts from bamboo, has very strong Anti-pathogenic Escherichia coli ability, can be used as the sterilization of romaine lettuce and cooker and the treatment of the infected.Bamboo extractive has good heat endurance, and fungistatic effect obviously strengthens after high temperature treatment, is more suitable for adding to need carrying out in the food of high temperature treatment.
The main component of licorice is licorice polysaccharide, and research shows, the MIC of licorice polysaccharide to Escherichia coli and staphylococcus aureus is 6.25%, is 12.5% to the MIC of salmonella, is 25% to the MIC of bacillus subtilis, black aspergillus and brewer's yeast.
Tea Polyphenols in tealeaves is natural, and the same with leaf of bamboo antioxidant have antibacterial antioxidation, and both share, and its antioxidation is 6 times of BHT, is 10 times of sodium ascorbate, and the color of food and nutrient component can be kept to remain unchanged for a long period of time.
Cajeputtree, lemon all have very abundant derived essential oil, triple combination use, not only there is antibacterial antisepsis, can also flu-prevention, to antiviral effect.Melaleuca oil is the most helpful to respiratory tract, can help prevention influenza, throat infection, mucous membrane inflammation.Lemon is a kind of potent Anti-bacterium essential oil, all has resistance to all contagiosity germs, and Anti-bacterium effect is more effective than a lot of chemosterilant, and volatile oil can stimulate leukocyte, strengthens and the ability protecting body resistance to infect.Ancient Egyptian utilizes essential oil to carry out preservative treatment very early, and Knob loch etc. studies the anti-microbial property of plant fragrance essential oil, thinks that solvability in aqueous phase and active ingredient in essential oil enter the ability of thalline through cell relevant.The class terpene of essential oil can reduce biomembranous stability, thus disturbs the enzymatic reaction of energy metabolism.
The advantage that the present invention possesses and effect: whole raw material of the present invention all derives from Chinese herbal medicine, fruits and vegetables and spice, belong to edible grade raw material, several extract carries out compatibility under certain ratio, not only strengthen fungistatic effect, also expand antimicrobial spectrum, to the common Escherichia coli of various food, bacillus, staphylococcus aureus, shigella dysenteriae, aspergillus flavus, black-koji mould has the long-term effect suppressed, also have the adding of Chinese herbal medicine antiviral, preventing cold, the effect of pharynx-clearing throat-benefiting, this preservative is not only used as food additives, also has certain medicine healthy sofa effect.Add in food and can prevent to get angry, adding energy anti-inflammatory anti-acne in cosmetics to, is a kind of mouthfeel Natural Food grade bacteriostatic agent that all good, effect is obvious, with low cost and antioxidant.
Embodiment
Below by embodiment, the present invention will be further described.
Embodiment 1
(1) get the raw materials ready by the component of following mass percent: bamboo extractive 40%, tea extract 30%, licorice 28%, lemon 1%, Melaleuca oil 1%, the summation of above each component is 100%;
Wherein, bamboo extractive obtains through the following step:
1. by dry for the leaf of bamboo, be crushed to 30 orders, then count 1:40 with distilled water with solid-to-liquid ratio kg/L and carry out immersion 25min, obtain leaf of bamboo soak; Add cellulase and the neutral proteinase of leaf of bamboo soak cumulative volume 0.3%, then use citric acid adjust pH to 4, then at 60 DEG C, heat 3h, refilter and obtain filtrate and filter residue;
2. gained filter residue repeats step operation 1. time, and merging filtrate, is concentrated into paste by filtrate, then dries at 80 DEG C, namely obtains bamboo extractive.
Tea extract obtains through the following step:
Tea leaf powder is broken to 30 orders, then counts 1:30 with distilled water with solid-to-liquid ratio kg/L and carry out immersion 30min, then decoct 2h and filter, repeat to soak and decoct operation 3 times, merging filtrate, by filtrate through being evaporated to paste, dry at 70 DEG C again, namely obtain tea extract.
Licorice obtains through the following step:
By dry for Radix Glycyrrhizae, be crushed to 30 orders, then count 1:40 with distilled water with solid-to-liquid ratio kg/L and carry out immersion 20min, then decoction 2h filtering, repeat to soak and decoct operation 3 times, merging filtrate, by filtrate through being evaporated to paste, dry at 70 DEG C again, namely obtain licorice.
Lemon obtains through the following step:
The distilled water adding lemon tablet weight 40 times in the lemon tablet dried in the shade carries out immersion 30min, obtain lemon tablet soak, add the beta-glucosidase of lemon tablet soak cumulative volume 0.5% again, and at 50 DEG C constant temperature 4h, then steam distillation is carried out, collect distillate by condenser, distillate cools layering at being placed in 3 DEG C, and upper strata is lemon.
Melaleuca oil obtains through the following step:
The distilled water adding cajeputtree weight 50 times in the cajeputtree dried in the shade carries out immersion 20min, obtain cajeputtree soak, add the beta-glucosidase of cajeputtree soak cumulative volume 0.8% again, and at 50 DEG C constant temperature 4h, then steam distillation is carried out, collect distillate by condenser, distillate cools layering at being placed in 3 DEG C, and upper strata is Melaleuca oil.
(2) bamboo extractive, tea extract and licorice in step (1) are mixed, then add lemon and Melaleuca oil, fully mixing absorbs completely to essential oil, namely obtains natural antiseptic agent.
Embodiment 2
(1) get the raw materials ready by the component of following mass percent: bamboo extractive 58%, tea extract 20%, licorice 20%, lemon 1%, Melaleuca oil 1%, the summation of above each component is 100%;
Wherein, bamboo extractive obtains through the following step:
1. by dry for the leaf of bamboo, be crushed to 20 orders, then count 1:50 with distilled water with solid-to-liquid ratio kg/L and carry out immersion 20min, obtain leaf of bamboo soak; Add cellulase and the neutral proteinase of leaf of bamboo soak cumulative volume 0.5%, then use citric acid adjust pH to 5, then at 50 DEG C, heat 2h, refilter and obtain filtrate and filter residue;
2. gained filter residue repeats step operation 1. 3 times, and merging filtrate, is concentrated into paste by filtrate, then dries at 70 DEG C, namely obtains bamboo extractive.
Tea extract obtains through the following step:
Tea leaf powder is broken to 40 orders, then counts 1:10 with distilled water with solid-to-liquid ratio kg/L and carry out immersion 20min, then decoct 2h and filter, repeat to soak and decoct operation 4 times, merging filtrate, by filtrate through being evaporated to paste, dry at 60 DEG C again, namely obtain tea extract.
Licorice obtains through the following step:
By dry for Radix Glycyrrhizae, be crushed to 40 orders, then count 1:10 with distilled water with solid-to-liquid ratio kg/L and carry out immersion 25min, then decoction 2h filtering, repeat to soak and decoct operation 4 times, merging filtrate, by filtrate through being evaporated to paste, dry at 60 DEG C again, namely obtain licorice.
Lemon obtains through the following step:
The distilled water adding lemon tablet weight 50 times in the lemon tablet dried in the shade carries out immersion 25min, obtain lemon tablet soak, add the beta-glucosidase of lemon tablet soak cumulative volume 0.8% again, and at 45 DEG C constant temperature 5h, then steam distillation is carried out, collect distillate by condenser, distillate cools layering at being placed in 4 DEG C, and upper strata is lemon.
Melaleuca oil obtains through the following step:
The distilled water adding cajeputtree weight 40 times in the cajeputtree dried in the shade carries out immersion 25min, obtain cajeputtree soak, add the beta-glucosidase of cajeputtree soak cumulative volume 0.5% again, and at 45 DEG C constant temperature 5h, then steam distillation is carried out, collect distillate by condenser, distillate cools layering at being placed in 4 DEG C, and upper strata is Melaleuca oil.
(2) bamboo extractive, tea extract and licorice in step (1) are mixed, then add lemon and Melaleuca oil, fully mixing absorbs completely to essential oil, namely obtains natural antiseptic agent.
Embodiment 3
(1) get the raw materials ready by the component of following mass percent: bamboo extractive 44%, tea extract 25%, licorice 30%, lemon 0.5%, Melaleuca oil 0.5%, the summation of above each component is 100%;
Wherein, bamboo extractive obtains through the following step:
1. by dry for the leaf of bamboo, be crushed to 40 orders, then count 1:10 with distilled water with solid-to-liquid ratio kg/L and carry out immersion 30min, obtain leaf of bamboo soak; Add cellulase and the neutral proteinase of leaf of bamboo soak cumulative volume 0.1%, then use citric acid adjust pH to 4, then at 80 DEG C, heat 3h, refilter and obtain filtrate and filter residue;
2. gained filter residue repeats step operation 1. 5 times, and merging filtrate, is concentrated into paste by filtrate, then dries at 60 DEG C, namely obtains bamboo extractive.
Tea extract obtains through the following step:
Tea leaf powder is broken to 20 orders, then counts 1:50 with distilled water with solid-to-liquid ratio kg/L and carry out immersion 25min, then decoct 2h and filter, repeat to soak and decoct operation 5 times, merging filtrate, by filtrate through being evaporated to paste, dry at 80 DEG C again, namely obtain tea extract.
Licorice obtains through the following step:
By dry for Radix Glycyrrhizae, be crushed to 20 orders, then count 1:50 with distilled water with solid-to-liquid ratio kg/L and carry out immersion 30min, then decoction 2h filtering, repeat to soak and decoct operation 5 times, merging filtrate, by filtrate through being evaporated to paste, dry at 80 DEG C again, namely obtain licorice.
Lemon obtains through the following step:
The distilled water adding lemon tablet weight 10 times in the lemon tablet dried in the shade carries out immersion 20min, obtain lemon tablet soak, add the beta-glucosidase of lemon tablet soak cumulative volume 1% again, and at 40 DEG C constant temperature 3h, then steam distillation is carried out, collect distillate by condenser, distillate cools layering at being placed in 4 DEG C, and upper strata is lemon.
Melaleuca oil obtains through the following step:
The distilled water adding cajeputtree weight 10 times in the cajeputtree dried in the shade carries out immersion 30min, obtain cajeputtree soak, add the beta-glucosidase of cajeputtree soak cumulative volume 1% again, and at 40 DEG C constant temperature 3h, then steam distillation is carried out, collect distillate by condenser, distillate cools layering at being placed in 4 DEG C, and upper strata is Melaleuca oil.
(2) bamboo extractive, tea extract and licorice in step (1) are mixed, then add lemon and Melaleuca oil, fully mixing absorbs completely to essential oil, namely obtains natural antiseptic agent.
Embodiment 4
(1) get the raw materials ready by the component of following mass percent: bamboo extractive 49.8%, tea extract 25%, licorice 25%, lemon 0.1%, Melaleuca oil 0.1%, the summation of above each component is 100%;
Wherein, bamboo extractive obtains through the following step:
1. by dry for the leaf of bamboo, be crushed to 20 orders, then count 1:50 with distilled water with solid-to-liquid ratio kg/L and carry out immersion 20min, obtain leaf of bamboo soak; Add cellulase and the neutral proteinase of leaf of bamboo soak cumulative volume 0.5%, then use citric acid adjust pH to 5, then at 50 DEG C, heat 2h, refilter and obtain filtrate and filter residue;
2. gained filter residue repeats step operation 1. 3 times, and merging filtrate, is concentrated into paste by filtrate, then dries at 70 DEG C, namely obtains bamboo extractive.
Tea extract obtains through the following step:
Tea leaf powder is broken to 40 orders, then counts 1:10 with distilled water with solid-to-liquid ratio kg/L and carry out immersion 20min, then decoct 2h and filter, repeat to soak and decoct operation 4 times, merging filtrate, by filtrate through being evaporated to paste, dry at 60 DEG C again, namely obtain tea extract.
Licorice obtains through the following step:
By dry for Radix Glycyrrhizae, be crushed to 40 orders, then count 1:10 with distilled water with solid-to-liquid ratio kg/L and carry out immersion 25min, then decoction 2h filtering, repeat to soak and decoct operation 4 times, merging filtrate, by filtrate through being evaporated to paste, dry at 60 DEG C again, namely obtain licorice.
Lemon obtains through the following step:
The distilled water adding lemon tablet weight 50 times in the lemon tablet dried in the shade carries out immersion 25min, obtain lemon tablet soak, add the beta-glucosidase of lemon tablet soak cumulative volume 0.8% again, and at 45 DEG C constant temperature 5h, then steam distillation is carried out, collect distillate by condenser, distillate cools layering at being placed in 4 DEG C, and upper strata is lemon.
Melaleuca oil obtains through the following step:
The distilled water adding cajeputtree weight 40 times in the cajeputtree dried in the shade carries out immersion 25min, obtain cajeputtree soak, add the beta-glucosidase of cajeputtree soak cumulative volume 0.5% again, and at 45 DEG C constant temperature 5h, then steam distillation is carried out, collect distillate by condenser, distillate cools layering at being placed in 4 DEG C, and upper strata is Melaleuca oil.
(2) bamboo extractive, tea extract and licorice in step (1) are mixed, then add lemon and Melaleuca oil, fully mixing absorbs completely to essential oil, namely obtains natural antiseptic agent.
Below the effect of gained natural antiseptic agent of the present invention is researched and analysed:
1, the bacteriostatic test of natural antiseptic agent provided by the invention
Adopt Odontothrips loti to measure the bacteriostatic activity of preservative of the present invention, concrete process of the test is as follows:
1.1 test method
Comparative example is set:
Comparative example 1: natural antiseptic agent only uses bamboo extractive for raw material;
Comparative example 2: natural antiseptic agent only uses tea extract for raw material;
Comparative example 3: natural antiseptic agent only uses licorice for raw material;
Comparative example 4: natural antiseptic agent only uses lemon for raw material;
Comparative example 5: natural antiseptic agent only uses Melaleuca oil for raw material;
Comparative example 6: preservative only uses commercial Sodium Benzoate for raw material; .
With sterile water, each comparative example is diluted to the various antiseptic solutions of mass fraction 4.000%, 2.000%, 1.000%, 0.500%, 0.250% and 0.125%; Gained natural antiseptic agent sterile water of the present invention is diluted to the natural anticorrosion agent solution of mass fraction 4.000%, 2.000%, 1.000%, 0.500%, 0.250% and 0.125%.
With staphylococcus aureus, Candida albicans, Pseudomonas aeruginosa, Escherichia coli, shigella dysenteriae, aspergillus flavus, aspergillus niger for strain subject, with sterile water, above-mentioned bacterial cell and fungal spore are diluted to respectively the cell suspension of OD660>0.5.Preparation LB medium and PDA medium, 121 DEG C of sterilizing 20min, are down flat plate (plate diameter is 90mm), to cooled and solidified, for subsequent use.Getting the above-mentioned bacterial cell suspension of 100 μ L is coated on the LB culture medium flat plate for preparing respectively equably, and fungal spore suspension is coated on the PDA culture medium flat plate for preparing respectively equably, an Oxford cup is placed in the flat board central authorities coating bacterium, add in the cup of Oxford by the antiseptic solution of above-mentioned variable concentrations and their composition 200 μ L respectively, 3 repetitions are all established in each process.To bacterial cell suspension be scribbled and the LB of adding preservative agent is dull and stereotyped as 37 DEG C of constant temperature culture, after 24h, measure inhibition zone size; And scribble fungal spore suspension and the PDA of adding preservative agent is dull and stereotyped as 28 DEG C of constant temperature culture, measure inhibition zone size after 48h.
1.2 evaluation method
Bacteriostatic diameter >=10mm is that effectively bacteriostatic diameter < 10mm is invalid.
1.3 result of the test
The routine inhibition zone size to strain subject of different tests is in table 1.
Table 1 comparative example and the present invention are to the antibacterial circle diameter (mm) for examination bacterium
Table 1 shows, comparative example 1 ~ 5 pair of staphylococcus aureus, Candida albicans, Pseudomonas aeruginosa, Escherichia coli, shigella dysenteriae, aspergillus flavus, aspergillus niger have inhibitory action in various degree, when each plant extracts is used alone, fungistatic effect is little compared with comparative example 6, but natural antiseptic agent fungistatic effect of the present invention is better than Sodium Benzoate, illustrate that the composition of natural plant extracts mixing composition can be used as preservative.Extract because natural antiseptic agent of the present invention belongs to pure natural plant, green, environmental protection, safety, can be used as food grade preservative.
According to evaluation method, bacteriostatic diameter >=10mm is effective, there is good inhibitory action to staphylococcus aureus, Candida albicans, Pseudomonas aeruginosa, shigella dysenteriae when so above-mentioned food-grade corrosion-resistant agent concentration is 0.250%, when concentration is 0.500%, have good inhibitory action to Escherichia coli, aspergillus flavus and aspergillus niger.
Claims (10)
1. a natural antiseptic agent, it is characterized in that being made up of the component of following masses percentage: bamboo extractive 40 ~ 58%, tea extract 20 ~ 30%, licorice 20 ~ 30%, lemon 0.1 ~ 1%, Melaleuca oil 0.1 ~ 1%, the summation of above each component is 100%.
2. natural antiseptic agent according to claim 1, is characterized in that: described bamboo extractive obtains through the following step:
1. by dry for the leaf of bamboo, be crushed to 20 ~ 40 orders, then count 1:10 ~ 50 with distilled water with solid-to-liquid ratio kg/L and carry out immersion 20 ~ 30min, obtain leaf of bamboo soak; Add the biology enzyme of leaf of bamboo soak cumulative volume 0.1 ~ 0.5%, then use citric acid adjust pH to 4 ~ 5, then at 50 ~ 80 DEG C, heat 2 ~ 3h, refilter and obtain filtrate and filter residue;
2. gained filter residue repeats step operation 1. 3 ~ 5 times, and merging filtrate, is concentrated into paste by filtrate, then dries at 60 ~ 80 DEG C, namely obtains bamboo extractive.
3. natural antiseptic agent according to claim 2, is characterized in that: described biology enzyme is cellulase and neutral proteinase, and cellulase and neutral proteinase are that arbitrary proportion adds.
4. natural antiseptic agent according to claim 1, is characterized in that: described tea extract obtains through the following step:
Tea leaf powder is broken to 20 ~ 40 orders, count 1:10 ~ 50 with distilled water with solid-to-liquid ratio kg/L again and carry out immersion 20 ~ 30min, then decoct 2h and filter, repeat to soak and decoct operation 3 ~ 5 times, merging filtrate, by filtrate through being evaporated to paste, then drying at 60 ~ 80 DEG C, namely obtaining tea extract.
5. natural antiseptic agent according to claim 1, is characterized in that: described licorice obtains through the following step:
By Radix Glycyrrhizae drying, be crushed to 20 ~ 40 orders, count 1:10 ~ 50 with distilled water with solid-to-liquid ratio kg/L again and carry out immersion 20 ~ 30min, then decoct 2h and filter, repeat to soak and decoct operation 3 ~ 5 times, merging filtrate, by filtrate through being evaporated to paste, then drying at 60 ~ 80 DEG C, namely obtaining licorice.
6. natural antiseptic agent according to claim 1, is characterized in that: described lemon obtains through the following step:
The distilled water adding lemon tablet weight 10 ~ 50 times in the lemon tablet dried in the shade carries out immersion 20 ~ 30min, obtain lemon tablet soak, add the cellulase of lemon tablet soak cumulative volume 0.5 ~ 1% again, and at 40 ~ 50 DEG C constant temperature 3 ~ 5h, then steam distillation is carried out, collect distillate by condenser, distillate cools layering at being placed in 3 ~ 4 DEG C, and upper strata is lemon.
7. natural antiseptic agent according to claim 1, is characterized in that: described Melaleuca oil obtains through the following step:
The distilled water adding cajeputtree weight 10 ~ 50 times in the cajeputtree dried in the shade carries out immersion 20 ~ 30min, obtain cajeputtree soak, add the cellulase of cajeputtree soak cumulative volume 0.5 ~ 1% again, and at 40 ~ 50 DEG C constant temperature 3 ~ 5h, then steam distillation is carried out, collect distillate by condenser, distillate cools layering at being placed in 3 ~ 4 DEG C, and upper strata is Melaleuca oil.
8. the natural antiseptic agent according to claim 6 or 7, is characterized in that: described cellulase is beta-glucosidase.
9. a preparation method for natural antiseptic agent, is characterized in that through following each step:
(1) get the raw materials ready by the component of following mass percent: bamboo extractive 40 ~ 58%, tea extract 20 ~ 30%, licorice 20 ~ 30%, lemon 0.1 ~ 1%, Melaleuca oil 0.1 ~ 1%, the summation of above each component is 100%;
(2) bamboo extractive, tea extract and licorice in step (1) are mixed, then add lemon and Melaleuca oil, fully mixing absorbs completely to essential oil, namely obtains natural antiseptic agent.
10. claim 1 ~ 7 natural antiseptic agent application in food, medicine, chemical industry, amenities, commodity, cosmetics described in any one, specifically adds by 0.05 ~ 3% of product quality.
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| CN110789850A (en) * | 2019-10-12 | 2020-02-14 | 宝应县永佳米业有限公司 | Germ rice fresh-keeping packaging storage method |
| CN111742980A (en) * | 2020-07-23 | 2020-10-09 | 武汉轻工大学 | Biofilm inhibitor and preparation method and application thereof |
| CN112021490A (en) * | 2020-09-18 | 2020-12-04 | 徐州工程学院 | Food preservative and preparation method and application thereof |
| CN114617217A (en) * | 2022-03-29 | 2022-06-14 | 陇东学院 | Thyme essential oil food preservative and preparation method thereof |
| CN117482020A (en) * | 2023-11-30 | 2024-02-02 | 广州茱颜化妆品有限公司 | Composition for repairing skin barrier and preparation method and application thereof |
| CN117482020B (en) * | 2023-11-30 | 2024-04-05 | 广州茱颜化妆品有限公司 | Composition for repairing skin barrier and preparation method and application thereof |
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