CN102757293B - Organic selenium-enzyme and preparation method thereof - Google Patents
Organic selenium-enzyme and preparation method thereof Download PDFInfo
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- CN102757293B CN102757293B CN2012102767930A CN201210276793A CN102757293B CN 102757293 B CN102757293 B CN 102757293B CN 2012102767930 A CN2012102767930 A CN 2012102767930A CN 201210276793 A CN201210276793 A CN 201210276793A CN 102757293 B CN102757293 B CN 102757293B
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Abstract
The invention discloses an organic selenium-enzyme. The content of protein is 25-40%, the content of activated carbon is 8-9.55, humus is smaller than or equal to 50%, Se is larger than or equal to 5%, and H2O is smaller than or equal to 10%. The invention further discloses a preparation method of the organic selenium-enzyme. The method includes mixing, by weight, 60-70 parts of dregs of rapeseeds, 30-40 parts of soybean meal and corn starch with equal proportion, and 1-3 parts of sucrose with water at a temperature of 110-120 DEG C for 120 minutes; cooling the mixture until the temperature is 40-50 DEG C, mixing 0.5-1.5 parts of by weight of yeast powder, placing the mixture into a thermostat at a temperature of 45 DEG C for 120 hours; mixing 0.5-1.5 parts of by weight of glucoamylase and 3-6 parts of by weight of K3SeP3O10.xH2O into the mixture, placing the mixture into a culture pot, placing the culture pot in a sterile workshop for culture at a room temperature of 25-35 DEG C; andplacing the mixture in a pressure kettle for vacuum degassing and dehumidification after 30 days to obtain the organic selenium-enzyme. The organic selenium-enzyme can activate microorganisms in soil, physical and mechanical states of the soil are obviously improved through metabolic turnover of the microorganisms, and the organic selenium-enzyme can be used as a micronutrient fertilizer for improving the soil.
Description
Technical field
The invention provides a kind of organoselenium enzyme and preparation method thereof.
Background technology
Since 1974, China's agroforestry are produced a large amount of chemical fertilizer that use, and have caused the increasingly deterioration of soil organisms and edatope, have produced many adverse consequencess: 1. soil compaction, soil erosion and desertification, 2. the pollution of heavy metal, the 3. decline of farm crop interior quality; The underproduction of unit surface, 4. the pollution of environment is to coercing of human health etc.How to get along amiably and peacefully with nature in the development of modern agricultural production science and technology, the sustainable development of protection Limited Land resource, promotion multi-form agriculture is the problem that we will ponder deeply and expect to solve.
At present, the selenium fertilizer of selling on the market mostly is inorganic selenium, it is Sodium Selenite, the Use Limitation valency is low, and life-time service can be to water quality and environment, the preparation method of existing a kind of organic selenium enzyme preparation, use the probiotic bacteriums such as fungi to be carrier, method by bio-transformation is converted into the low conversion rate of organoselenium to inorganic selenium, only has 30%～60%, affects result of use.Application number is the preparation method that 200910231110.8 file has been announced a kind of organic selenium enzyme preparation, adopts bacterial classification to cultivate for three times, and inorganic selenium is converted into organoselenium, but low conversion rate, and residual inorganic selenium is harmful to for soil microorganisms.
The present invention relates to a kind of yeast powder, saccharifying enzyme used as starter, be that 200610136924X " the phosphoric acid selenium potassium mixture " announced is as catalyzer with the patent No., with the albumen selenium enzyme that powder ﹑ cake-shaped rape seed dregs precursor logical everfermentation ﹑ enzymolysis metabolism in shallow lake forms, it is used as little fertilizer of improveing soil.Utilize it to activate microorganism in soil and harmless to soil microorganisms, by the microbial metabolism conversion, make the soil physical and chemical states obtain obviously improvement.
Summary of the invention
Purpose of the present invention aims to provide a kind of organoselenium enzyme, and it can well be applied to soil improvement.
The objective of the invention is to realize by following manner: a kind of organoselenium enzyme, each component concentration is: protein is 25～40%, activated carbon is 8～9.5%, humus≤50%, Se 〉=5%, H
2O≤10%, described albumen selenium enzyme is brown ceramic powder, microscopically has glomerocryst to exist, and a kind of special ferment flavor is arranged; Solvend 〉=50%.
Another object of the present invention is to provide the preparation method of above-mentioned organoselenium enzyme, may further comprise the steps: with 60～70 weight part cake-shaped rape seed dregs, 30～40 weight parts by the analysis for soybean powder of equal proportion proportioning and W-Gum, 1～3 weight part sucrose, blunge evenly by 110～120 ℃, processed in 120 minutes; Be cooled to 40～50 ℃, admix 0.5～1.5 weight part yeast powder, enter 45 ℃ of thermostat containers, fermentation in 120 hours; In whipping process, 0.5～1.5 weight part saccharifying enzyme and 3～6 weight part phosphoric acid selenium potassium solutions are admixed wherein equably; The dress Culture basin is put sterile workshop training and is grown, and room temperature keeps 25～35 ℃, enters the autoclave pressure vacuum outgas after 30 days, dehumidifying forms.
The starter that the present invention selects is yeast powder, saccharifying enzyme, and catalyzer is phosphoric acid selenium potassium, and fermentation substrate is cake-shaped rape seed dregs, analysis for soybean powder, W-Gum, sucrose.
The invention has the beneficial effects as follows: organoselenium enzyme of the present invention can significantly be urged to reach the purpose of improving soil fertility with the soil microorganisms breeding, is of value to crop growth.
Embodiment
Embodiment 1.
Machine selenium enzyme of the present invention adopts raw material to be: 70 kilograms of the thin cake-shaped rape seed dregs of 100 orders, 15 kilograms of analysis for soybean powder, 15 kilograms of W-Gums, 2 kilograms of sucrose, 1.5 kilograms of yeast powders, 1.5 kilograms in saccharifying enzyme, 50 liters of clean waters, 5 kilograms in phosphoric acid selenium potassium.
Cake-shaped rape seed dregs, analysis for soybean powder, W-Gum, sucrose are blunged evenly by 120 ℃, 120 minutes pyroprocessing materials, be cooled to 50 ℃, admix yeast powder and enter 45 ℃ of thermostat containers, after fermentation in 120 hours, in stirring, saccharifying enzyme and phosphoric acid selenium potassium solution are admixed wherein equably, the dress Culture basin, put sterile workshop training and grow, room temperature keeps 25 ℃, enters the autoclave pressure vacuum outgas after 30 days, dehumidifying forms.
Organoselenium enzyme of the present invention is checked through Hunan Province Institute of Analysis, test basis is GB/T 8572-2010 " mensuration that contains nitrogen content in the composite fertilizer ", GB/T 23349-2009 " arsenic in the fertilizer, plumbous chromium, cadmium, the mensuration of mercury ecological index ", GB18877(5.10)-2009 " mensuration of total humic acid in the organic-inorganic complex fertilizer ", Interventions Requested are humus, water-insoluble, moisture content, activated carbon, selenium, protein, assay are that selenium is 6.44%, and water-insoluble is 63.49%, moisture content 9.82%, humic acids is 23.41%, and activated carbon is 8.91%, and protein is 29.63%.
Embodiment 2.
Machine selenium enzyme of the present invention adopts raw material to be: 60 kilograms of the thin cake-shaped rape seed dregs of 100 orders, 20 kilograms of analysis for soybean powder, 20 kilograms of W-Gums, 2 kilograms of sucrose, 1 kilogram of yeast powder, 1 kilogram in saccharifying enzyme, 50 liters of clean waters, 6 kilograms in phosphoric acid selenium potassium.
Cake-shaped rape seed dregs, analysis for soybean powder, W-Gum, sucrose are blunged evenly by 120 ℃, the pyroprocessing material was cooled to 50 ℃ in 120 minutes, admix yeast powder and enter 45 ℃ of thermostat containers, after fermentation in 120 hours, in stirring saccharifying enzyme and phosphoric acid selenium potassium solution are admixed wherein equably, the dress Culture basin is put the sterile workshop training and is grown, room temperature keeps 35 ℃, enters the autoclave pressure vacuum outgas after 30 days, dehumidifying forms.
Embodiment 3.
Organoselenium enzyme of the present invention adopts raw material to be: 70 kilograms of the thin cake-shaped rape seed dregs of 100 orders, 15 kilograms of analysis for soybean powder, 15 kilograms of W-Gums, 2 kilograms of sucrose, 0.8 kilogram of yeast powder, 0.7 kilogram in saccharifying enzyme, 50 liters of clean waters, 5 kilograms in phosphoric acid selenium potassium.
Cake-shaped rape seed dregs, analysis for soybean powder, W-Gum, sucrose are blunged evenly by 120 ℃, the pyroprocessing material was cooled to 50 ℃ in 120 minutes, admix yeast powder and enter 45 ℃ of thermostat containers, after fermentation in 120 hours, in stirring saccharifying enzyme and phosphoric acid selenium potassium solution are admixed wherein equably, the dress Culture basin is put the sterile workshop training and is grown, room temperature keeps 30 ℃, enters the autoclave pressure vacuum outgas after 30 days, dehumidifying forms.
The above; only for the better embodiment of the present invention, but protection scope of the present invention is not limited to this, anyly is familiar with those skilled in the art in the technical scope that the present invention discloses; the variation that can expect easily or replacement all should be encompassed within protection scope of the present invention.Therefore, protection scope of the present invention should be as the criterion with the protection domain of claims.
Claims (2)
1. the preparation method of an organoselenium enzyme is characterized in that, with the analysis for soybean powder of the equal proportion proportioning of 60～70 weight part cake-shaped rape seed dregs, 30～40 weight parts and W-Gum, 1～3 weight part sucrose, blunges evenly by 110～120 ℃, processes in 120 minutes; Be cooled to 40～50 ℃, admix 0.5～1.5 weight part yeast powder, 0.5～1.5 weight part saccharifying enzyme and 3～6 weight part phosphoric acid selenium potassium, enter 45 ℃ of thermostat containers, fermentation in 120 hours; The dress Culture basin is put sterile workshop training and is grown, and room temperature keeps 25～35 ℃, enters the autoclave pressure vacuum outgas after 30 days, dehumidifying forms.
2. the preparation method of a kind of organoselenium enzyme according to claim 1, it is characterized in that the weight part of material is preferably: 1～2 weight part sucrose, 0.8～1 weight part yeast powder, 0.5～0.8 weight part saccharifying enzyme, 4～5 weight part phosphoric acid selenium potassium, 70 weight part cake-shaped rape seed dregs, 15 weight part soya beans, 15 weight part W-Gums.
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| CN105432319A (en) * | 2015-01-13 | 2016-03-30 | 罗大全 | Biological selenium-enriched fungi breeding method |
| CN110317100A (en) * | 2019-07-22 | 2019-10-11 | 唐山津国农业技术开发有限公司 | The production technology of organic selenium Liquid Fertilizer |
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