CN102084935A - Preparation method of organic selenium enzyme preparation - Google Patents
Preparation method of organic selenium enzyme preparation Download PDFInfo
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- CN102084935A CN102084935A CN2009102311108A CN200910231110A CN102084935A CN 102084935 A CN102084935 A CN 102084935A CN 2009102311108 A CN2009102311108 A CN 2009102311108A CN 200910231110 A CN200910231110 A CN 200910231110A CN 102084935 A CN102084935 A CN 102084935A
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Abstract
The invention provides a preparation method of an organic selenium enzyme preparation, comprising the following steps of: mixing bran, bean pulp, rice hull powder, a sodium selenite solution, and the like, sterilizing at high temperature and high pressure, inoculating a kojitray strain of candida utilis after sterilization, culturing and then taking out a material obtained through culturing, inoculating a kojitray strain of aspergillus usamii on the material obtained through culturing, culturing for the second time, inoculating a kojitray strain of bacillus subtilis on a material obtained through culturing for the second time, culturing for the third time, drying and crushing a material obtained through culturing for the third time to prepare the organic selenium enzyme preparation. In the preparation method of the organic selenium enzyme preparation, continuous three times of conventions are carried out by adopting three types of strains, the conversion ratio of converting original inorganic selenium with extremely low valence into organic selenium with higher valence can reach above 98 percent, and the prepared enzyme preparation integrates organic trace elements, enzyme preparation and probiotic bacteria into a whole, simultaneously has a plurality of biological functions and has the efficacy of reducing anti-nutritional factors.
Description
Technical field
The invention provides a kind of preparation method of organic selenium enzyme preparation.
Background technology
At present, the selenium product of selling on the feed addictive market mostly is inorganic selenium, i.e. sodium selenite, and the Use Limitation valency is lower, and long-term use meeting pollutes water quality and environment.Though some organic selenium mostly is the synthetic product of industry, cost is higher, and price is more expensive.The preparation method that a kind of organic selenium enzyme preparation is arranged now is to be carrier with probios such as fungi, bacteriums, by bio-transformation inorganic selenium is converted into organic selenium, has improved and has tired, the pollution that has caused when having avoided inorganic selenium to use.But this preparation method only once transforms with a kind of bacterium usually, and the conversion ratio that inorganic selenium is converted into organic selenium is low, has only 30%～60%, influences result of use.
Summary of the invention
The present invention wants the technical solution problem to be, a kind of preparation method of organic selenium enzyme preparation is provided, to improve the conversion ratio that inorganic selenium is converted into organic selenium.
The present invention realizes like this, a kind of preparation method of organic selenium enzyme preparation, described method is, press wheat bran 81～85 weight portions, dregs of beans 11～13 weight portions, powdered rice hulls 0.9～1.1 weight portion, ammonium sulfate 0.4～0.6 weight portion, potassium dihydrogen phosphate 0.4～0.6 weight portion, Fructus Hordei Germinatus 2～4 weight portions, mass percent concentration is that the proportioning of 0.1～0.2% sodium selenite solution 90～110 weight portions gets compound with above-mentioned mixing of materials, after stirring, when mixture moisture content is not enough, with sterilized water to the compound supplementing water, making its water content is 55～65% by weight, then, at pressure is 0.18～0.22MPa, temperature is under 115～125 ℃ the condition, carry out 43～46 minutes autoclave sterilization, after the sterilization, when above-mentioned material is cooled to 36～38 ℃, inoculate the koji tray bacterial classification of 2.3～2.7% candida utili (Candida utilis) of its weight, under 27～29 ℃ of temperature, cultivated 31～33 hours after stirring, to cultivate the gained material then takes out, with sterilized water to cultivating gained material supplementing water, making the water content of cultivating gains is 55～65% by weight, again on the cultivation gained material after the supplementing water, inoculate the koji tray bacterial classification of 0.9～1.1% Aspergillus usamii (Aspergillus usanii) of its weight, under 36～37 ℃ of temperature, cultivated once more 47～49 hours after stirring, to cultivate the gained material then once more takes out, when cultivating gained material moisture deficit once more, with sterilized water to cultivating gained material supplementing water once more, making the water content of cultivating gains once more is 55～65% by weight, on the gained of the cultivation once more material after cultivating gained material or supplementing water once more, inoculate the koji tray bacterial classification of 0.4～0.6% bacillus subtilis (Bacillus sutilis) of its weight, under 36～37 ℃ of temperature, cultivated for the third time 23～25 hours after stirring, to cultivate the gained drying materials for the third time, pulverize, cross 80～100 mesh sieves, make organic selenium enzyme preparation.
The preparation method of this organic selenium enzyme preparation, adopting three kinds of bacterium to carry out continuous three times transforms, inorganic selenium is converted into the conversion ratio height of organic selenium, can reach more than 98%, the extremely low inorganic selenium of originally tiring is converted into the higher organic selenium of tiring, enzyme preparation collection organic trace element, enzyme preparation, the probio of making are one, have both the various biological function, have the effect that reduces ANFs,, can make animal product have original, unique palatability as behind the additive of feed.
The specific embodiment
Further specify the present invention below.
Described koji tray bacterial classification is also referred to as the plate bacterial classification, after to be bacterial classification from test tube change triangular flask over to and enlarge cultivation, changes koji tray over to from triangular flask again and enlarges and cultivate the bacterial classification that obtains.
In the time of will cultivating the gained drying materials for the third time, its water content can be reduced to is 2%～10% by weight.
Through testing, organic selenium enzyme preparation of the present invention's method preparation can suppress the generation of small white mouse tumour significantly, shows its effect to having raising immunity of organisms, promotion to grow for the examination animal.
Embodiment 1
A kind of preparation method of organic selenium enzyme preparation, described method is, press wheat bran 81 weight portions, dregs of beans 13 weight portions, powdered rice hulls 0.9 weight portion, ammonium sulfate 0.6 weight portion, potassium dihydrogen phosphate 0.4 weight portion, Fructus Hordei Germinatus 4 weight portions, mass percent concentration is that the proportioning of 0.1% sodium selenite solution 110 weight portions gets compound with above-mentioned mixing of materials, after stirring, when mixture moisture content is not enough, with sterilized water to the compound supplementing water, making its water content is 65% by weight, then, at pressure is 0.18MPa, temperature is under 125 ℃ the condition, carry out 43 minutes autoclave sterilization, after the sterilization, when above-mentioned material is cooled to 38 ℃, inoculate the koji tray bacterial classification of 2.3% candida utili (Candida utilis) of its weight, under 29 ℃ of temperature, cultivated 31 hours after stirring, to cultivate the gained material then takes out, with sterilized water to cultivating gained material supplementing water, making the water content of cultivating gains is 65% by weight, again on the cultivation gained material after the supplementing water, inoculate the koji tray bacterial classification of 0.9% Aspergillus usamii (Aspergillus usanii) of its weight, under 37 ℃ of temperature, cultivated once more 47 hours after stirring, to cultivate the gained material then once more takes out, when cultivating gained material moisture deficit once more, with sterilized water to cultivating gained material supplementing water once more, making the water content of cultivating gains once more is 65% by weight, on the gained of the cultivation once more material after cultivating gained material or supplementing water once more, inoculate the koji tray bacterial classification of 0.4% bacillus subtilis (Bacillus sutilis) of its weight, under 37 ℃ of temperature, cultivated for the third time 23 hours after stirring, to cultivate the gained drying materials for the third time, pulverize, cross 100 mesh sieves, make organic selenium enzyme preparation.
Embodiment 2
A kind of preparation method of organic selenium enzyme preparation, described method is, press wheat bran 85 weight portions, dregs of beans 11 weight portions, powdered rice hulls 1.1 weight portions, ammonium sulfate 0.4 weight portion, potassium dihydrogen phosphate 0.6 weight portion, Fructus Hordei Germinatus 2 weight portions, mass percent concentration is that the proportioning of 0.2% sodium selenite solution 90 weight portions gets compound with above-mentioned mixing of materials, after stirring, when mixture moisture content is not enough, with sterilized water to the compound supplementing water, making its water content is 55% by weight, then, at pressure is 0.22MPa, temperature is under 115 ℃ the condition, carry out 46 minutes autoclave sterilization, after the sterilization, when above-mentioned material is cooled to 36 ℃, inoculate the koji tray bacterial classification of 2.7% candida utili (Candida utilis) of its weight, under 27 ℃ of temperature, cultivated 33 hours after stirring, to cultivate the gained material then takes out, with sterilized water to cultivating gained material supplementing water, making the water content of cultivating gains is 55% by weight, again on the cultivation gained material after the supplementing water, inoculate the koji tray bacterial classification of 1.1% Aspergillus usamii (Aspergillus usanii) of its weight, under 36 ℃ of temperature, cultivated once more 49 hours after stirring, to cultivate the gained material then once more takes out, when cultivating gained material moisture deficit once more, with sterilized water to cultivating gained material supplementing water once more, making the water content of cultivating gains once more is 55% by weight, on the gained of the cultivation once more material after cultivating gained material or supplementing water once more, inoculate the koji tray bacterial classification of 0.6% bacillus subtilis (Bacillus sutilis) of its weight, under 36 ℃ of temperature, cultivated for the third time 25 hours after stirring, to cultivate the gained drying materials for the third time, pulverize, cross 80 mesh sieves, make organic selenium enzyme preparation.
Embodiment 3
A kind of preparation method of organic selenium enzyme preparation, described method is, press wheat bran 83 weight portions, dregs of beans 12 weight portions, powdered rice hulls 1.0 weight portions, ammonium sulfate 0.5 weight portion, potassium dihydrogen phosphate 0.5 weight portion, Fructus Hordei Germinatus 3 weight portions, mass percent concentration is that the proportioning of 0.15% sodium selenite solution 100 weight portions gets compound with above-mentioned mixing of materials, after stirring, when mixture moisture content is not enough, with sterilized water to the compound supplementing water, making its water content is 60% by weight, then, at pressure is 0.20MPa, temperature is under 120 ℃ the condition, carry out 45 minutes autoclave sterilization, after the sterilization, when above-mentioned material is cooled to 37 ℃, inoculate the koji tray bacterial classification of 2.5% candida utili (Candida utilis) of its weight, under 28 ℃ of temperature, cultivated 32 hours after stirring, to cultivate the gained material then takes out, with sterilized water to cultivating gained material supplementing water, making the water content of cultivating gains is 60% by weight, again on the cultivation gained material after the supplementing water, inoculate the koji tray bacterial classification of 1.0% Aspergillus usamii (Aspergillus usanii) of its weight, under 36.5 ℃ of temperature, cultivated once more 48 hours after stirring, to cultivate the gained material then once more takes out, when cultivating gained material moisture deficit once more, with sterilized water to cultivating gained material supplementing water once more, making the water content of cultivating gains once more is 60% by weight, on the gained of the cultivation once more material after cultivating gained material or supplementing water once more, inoculate the koji tray bacterial classification of 0.5% bacillus subtilis (Bacillus sutilis) of its weight, under 36.5 ℃ of temperature, cultivated for the third time 24 hours after stirring, to cultivate the gained drying materials for the third time, pulverize, cross 90 mesh sieves, make organic selenium enzyme preparation.
Claims (1)
1. the preparation method of an organic selenium enzyme preparation, it is characterized in that, described method is, press wheat bran 81～85 weight portions, dregs of beans 11～13 weight portions, powdered rice hulls 0.9～1.1 weight portion, ammonium sulfate 0.4～0.6 weight portion, potassium dihydrogen phosphate 0.4～0.6 weight portion, Fructus Hordei Germinatus 2～4 weight portions, mass percent concentration is that the proportioning of 0.1～0.2% sodium selenite solution 90～110 weight portions gets compound with above-mentioned mixing of materials, after stirring, when mixture moisture content is not enough, with sterilized water to the compound supplementing water, making its water content is 55～65% by weight, then, at pressure is 0.18～0.22MPa, temperature is under 115～125 ℃ the condition, carry out 43～46 minutes autoclave sterilization, after the sterilization, when above-mentioned material is cooled to 36～38 ℃, inoculate the koji tray bacterial classification of 2.3～2.7% candida utili (Candida utilis) of its weight, under 27～29 ℃ of temperature, cultivated 31～33 hours after stirring, to cultivate the gained material then takes out, with sterilized water to cultivating gained material supplementing water, making the water content of cultivating gains is 55～65% by weight, again on the cultivation gained material after the supplementing water, inoculate the koji tray bacterial classification of 0.9～1.1% Aspergillus usamii (Aspergillus usanii) of its weight, under 36～37 ℃ of temperature, cultivated once more 47～49 hours after stirring, to cultivate the gained material then once more takes out, when cultivating gained material moisture deficit once more, with sterilized water to cultivating gained material supplementing water once more, making the water content of cultivating gains once more is 55～65% by weight, on the gained of the cultivation once more material after cultivating gained material or supplementing water once more, inoculate the koji tray bacterial classification of 0.4～0.6% bacillus subtilis (Bacillus sutilis) of its weight, under 36～37 ℃ of temperature, cultivated for the third time 23～25 hours after stirring, to cultivate the gained drying materials for the third time, pulverize, cross 80～100 mesh sieves, make organic selenium enzyme preparation.
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| CN2009102311108A CN102084935B (en) | 2009-12-04 | 2009-12-04 | Preparation method of organic selenium enzyme preparation |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102757293A (en) * | 2012-08-06 | 2012-10-31 | 长沙福山农业科技有限公司 | Organic selenium-enzyme and preparation method thereof |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1483340A (en) * | 2002-09-17 | 2004-03-24 | 祝光伟 | Protein single fodder containing microelement chelate and preparation process thereof |
| CN1582707A (en) * | 2003-08-18 | 2005-02-23 | 汤珍山 | Selenium containing microopganic additive for forage and its preparation |
| CN1709133A (en) * | 2005-07-12 | 2005-12-21 | 南京农业大学 | Selenium-rich probiotics foder additive production method and its product |
| CN101491289A (en) * | 2008-12-25 | 2009-07-29 | 南京财经大学 | Rape seed protein feedstuff and preparation method thereof |
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- 2009-12-04 CN CN2009102311108A patent/CN102084935B/en not_active Expired - Fee Related
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1483340A (en) * | 2002-09-17 | 2004-03-24 | 祝光伟 | Protein single fodder containing microelement chelate and preparation process thereof |
| CN1582707A (en) * | 2003-08-18 | 2005-02-23 | 汤珍山 | Selenium containing microopganic additive for forage and its preparation |
| CN1709133A (en) * | 2005-07-12 | 2005-12-21 | 南京农业大学 | Selenium-rich probiotics foder additive production method and its product |
| CN101491289A (en) * | 2008-12-25 | 2009-07-29 | 南京财经大学 | Rape seed protein feedstuff and preparation method thereof |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102757293A (en) * | 2012-08-06 | 2012-10-31 | 长沙福山农业科技有限公司 | Organic selenium-enzyme and preparation method thereof |
| CN102757293B (en) * | 2012-08-06 | 2013-10-23 | 长沙福山农业科技有限公司 | Organic selenium-enzyme and preparation method thereof |
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