CN1579195A - High-active protein and technology for producing said protein by multiple bacterial-spawn liquid-solid combined fermentation - Google Patents

Abstract

The invention is a highly active protein, taking multiple agricultural and sideline products as raw materials, inoculating multiple bacterial strains and adopting liquid-solid combined fermenting process to make it. Its raw materials includes: bran, aginomoto residue, beer residue, vermicelli protein flour, and corn protein flour; and the fermenting bacterial strains: lipolytic Candida, rice aspergillus, deproteinizing Candida, black mold and Geotrichum candidum. The multi-bacterial strain liquid- solid combined fermenting process to produce highly active protein has a flow: (1) inclining bacterial strains; (2) shaking bottle to culture; (3) culturing in fermentation cylinder; (4) weighing, mixing and adding water of raw materials; (5) curing raw materials; (6) inoculating liquid bacterial strains; (7) solid-fermenting; (8) drying and crushing; (9) measuring and packaging. The highly active protein has high amino acid content, especially the number of two most important ones-lysine and methionine and that of yeast active cells are large, therefore the proportion of it replacing import fish protein concentrate can be increased from <40% to 60-80%.

Description

Highly active protein and many strain liquids connect firmly the fermenting and producing highly active protein technology of closing

Affiliated technical field

The present invention relates to feed addictive, refer to a kind of highly active protein and production technology thereof of alternative imported fish meal especially.

Background technology

Imported fish meal is a kind of animal protein, because of biologically active, be easy to be absorbed, thereby its biological value is higher than other vegetable protein by livestock and poultry, aquatic livestock, be a kind of important feed addictive, in livestock and poultry, aquaculture, be used as the important source of protein.But since the in short supply and important function on aquaculture of fish meal resource its hold at high price, the work of therefore developing the substitute of fish meal comes into one's own always.Usual way be with vegetable protein by microbial fermentation manufacture order cell protein, as with powdered rice hulls, residue of monosodium glutamate, schlempe, starch wastewater or starch wastewater, or oil is a raw material.But the product of these method gained is compared with imported fish meal, be that crude protein or amino acid balance aspect all have suitable gap, especially two kinds of most important amino acid lysines and methionine content are more inadequate, and be also not high enough aspect enzymatic activity and yeast living cells.

Summary of the invention

The object of the invention is: utilize the amino acid whose complementarity that exists between the multiple agricultural byproducts raw material, by screening to microorganism fungus kind, adopt many strain liquids to connect firmly combined fermentation technology, utilize the collaborative short relation of giving birth between each bacterial classification, make the starch in the agricultural byproducts raw material, fat, protein is farthest assimilated, improve the utilization rate of vegetable protein, produce a kind of highly active protein, make wherein essential amino acid content near imported fish meal, the key technical indexes and biological effectiveness reach or near imported fish meal, part substitutes imported fish meal in the aquaculture to be implemented in, thereby solves imported fish meal resource scarcity and the high problem of cost.

A kind of highly active protein of the present invention, adopting multiple agricultural byproducts is raw material, by inoculating many bacterial classifications, and adopts liquid-solid combined ferment technology to make;

Its composition of raw materials is: wheat bran 5-15%, and residue of monosodium glutamate 20-38%, beer slag 10-20%, vermicelli protein powder 20-35%, all the other are corn protein powder;

Wherein fermented bacterium adopts: Candida lipolytica 10-30%, and aspergillus oryzae 10-30%, proteolysis Candida 10-25%, aspergillus niger 10-25%, all the other are geotrichum candidum.

The flow process that many strain liquids of the present invention connect firmly combined fermentation technology production highly active protein is:

(1) slant strains; (2) shaking bottle cultivates; (3) fermentation tank culture; (4) water is weighed, mixes, added to raw material; (5) raw material slaking; (6) inoculation liquid spawn; (7) solid fermentation; (8) oven dry, pulverizing; (9) metering, packing.

Highly active protein of the present invention has following characteristics: (1) amino acid content height, these two kinds most important amino acid content height of lysine and methionine particularly, bring up to 3.2-3.6% as lysine content from existing＜2.0%, methionine content is brought up to 1.66-1.80% from existing＜1.0%; (2) the yeast viable count is from existing＜10 ⁸Bring up to＞10 ⁹(individual/gram).Therefore the ratio of its alternative imported fish meal can be brought up to 60-80% from present＜40%.

Owing to adopt multiple agricultural byproducts raw material and many strain liquids to connect firmly the highly active protein that combined fermentation technology is produced, because of utilizing the collaborative short relation of giving birth between each bacterial classification, improved the bioavailability of raw material, starch, fat, protein in the agricultural byproducts raw material are farthest assimilated, improved the utilization rate of vegetable protein, reach in the product biological essential amino acid content near imported fish meal, therefore imported fish meal be can substitute as feed addictive, thereby imported fish meal resource scarcity problem and the high problem of cost solved.

Description of drawings

Fig. 1 is the technological process of production figure of highly active protein of the present invention.

The specific embodiment

Highly active protein of the present invention makes full use of the amino acid whose complementarity that exists between the various agricultural byproducts raw materials on prescription, the design science prescription, make product on amino acid is formed near imported fish meal, some vitamin ingredients is better than imported fish meal.

Composition of raw materials is exemplified below: by weight (%)

Material name	Embodiment 1	Embodiment 2	Embodiment 3	Embodiment 4
					Wheat bran	??6.5	??8.3	??12.2	??14.0
Residue of monosodium glutamate	??36.3	??30.2	??25.8	??22.0
					The beer slag	??10.8	??13.0	??17.5	??19.3
The vermicelli protein powder	??33.3	??26.4	??24.1	??20.5
					Corn protein powder	All the other	All the other	All the other	All the other

After pressing the formula, fermenting of embodiment, amino acid content in the finished product such as following table.This product animal experiment shows, with 50-80% substituting import one fish meal feed pig and fryer, its feeding effect is similar.

Existing combined process flow chart is described further the flow process of explained hereafter highly active protein of the present invention.

1. slant strains: the formulated culture medium by following culture medium, be made into some inclined-planes, bacterial classification is seeded to each inclined-plane in proportion respectively, in biochemical incubator, cultivated 24-72 hour.The slant medium prescription is a potato 20%, glucose 2%, and agar 1.8% adds water to 100%.

Used bacterial classification is: Candida lipolytica 10-30%, and aspergillus oryzae 10-30%, proteolysis Candida 10-25%, aspergillus niger 10-25%, all the other are geotrichum candidum.

2. shaking bottle cultivates: the fluid nutrient medium branch is packed into 10 shake in the bottle, inoculate 5 bacterial strains then respectively, will shake bottle and place constant temperature oscillator to cultivate 24-72 hour.

The Liquid Culture based formulas of using when bacterial classification is made is: potato 20%, and glucose 2%, potassium dihydrogen phosphate 0.5%, magnesium sulfate 0.25% adds water to 100%.

3. fermentation tank culture: be the output that enlarges bacterial classification, adopt the 30L fermentation tank to produce liquid spawn, its technology is: preparation 20L fluid nutrient medium, pour in the jar, machine, inoculate and shake the first class inoculum that the bottle cultivation is produced, temperature conditions is 30 ℃, cultivates 24h.

4. water is weighed, mixes, added to raw material: by each embodiment in the following formula table, preparation raw material mixes, by adding water at 1: 1.

5. raw material slaking: with above-mentioned confected materials high-temperature sterilization 30 minutes.

6. inoculation liquid spawn: 5 bacterial strains of 1 described ratio inoculation set by step after the raw material cooling.

7. solid fermentation: begin fermentation, treat that white hypha is covered with whole raw material surface after, stir once.Fermentation in about 12 hours is finished, and promptly obtains primary product.

8. oven dry, pulverizing: primary product are dried, pulverize.

9. metering, packing.

Claims (4)

1. highly active protein, adopting multiple agricultural byproducts is raw material, by inoculating many bacterial classifications, and adopts liquid-solid combined ferment technology to make;

Its raw material adopts: wheat bran, residue of monosodium glutamate, beer slag, vermicelli protein powder and corn protein powder;

Fermented bacterium adopts Candida lipolytica, aspergillus oryzae, proteolysis Candida, aspergillus niger and geotrichum candidum.

2. highly active protein according to claim 1 is characterized in that described composition of raw materials is: wheat bran 5-15%, and residue of monosodium glutamate 20-38%, beer slag 10-20%, vermicelli protein powder 20-35%, all the other are corn protein powder.

3. highly active protein according to claim 1 is characterized in that the ratio of described fermented bacterium is: Candida lipolytica 10-30%, and aspergillus oryzae 10-30%, proteolysis Candida 10-25%, aspergillus niger 10-25%, all the other are geotrichum candidum.

4. strain liquid connects firmly the technology of closing the fermenting and producing highly active protein more than one kind, and its flow process is:

(1) slant strains; (2) shaking bottle cultivates; (3) fermentation tank culture; (4) water is weighed, mixes, added to raw material; (5) raw material slaking; (6) inoculation liquid spawn; (7) solid fermentation; (8) oven dry, pulverizing; (9) metering, packing.

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